AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57443

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57443.t1 MTR_2g010270 89.674 184 13 2 1 184 98 275 8.40e-113 328
MS.gene57443.t1 MTR_7g024060 83.420 193 15 3 1 193 117 292 3.57e-106 311
MS.gene57443.t1 MTR_6g087820 83.420 193 15 3 1 193 117 292 3.57e-106 311
MS.gene57443.t1 MTR_5g081190 83.420 193 15 3 1 193 117 292 3.57e-106 311
MS.gene57443.t1 MTR_5g091480 83.420 193 15 3 1 193 117 292 3.57e-106 311
MS.gene57443.t1 MTR_5g056290 83.420 193 15 3 1 193 117 292 3.57e-106 311
MS.gene57443.t1 MTR_3g031190 83.420 193 15 3 1 193 117 292 3.57e-106 311
MS.gene57443.t1 MTR_2g005490 83.420 193 15 3 1 193 117 292 3.57e-106 311
MS.gene57443.t1 MTR_2g010570 83.420 193 15 3 1 193 117 292 3.57e-106 311
MS.gene57443.t1 MTR_5g030110 83.420 193 15 3 1 193 117 292 3.77e-106 311
MS.gene57443.t1 MTR_2g007410 83.420 193 15 3 1 193 117 292 3.77e-106 311
MS.gene57443.t1 MTR_8g446640 85.870 184 11 2 1 184 98 266 1.55e-105 309
MS.gene57443.t1 MTR_7g451880 83.696 184 15 2 1 184 118 286 9.92e-104 305
MS.gene57443.t1 MTR_7g074180 83.696 184 15 2 1 184 117 285 3.29e-103 304
MS.gene57443.t1 MTR_4g058660 83.152 184 16 2 1 184 117 285 1.33e-102 302
MS.gene57443.t1 MTR_1g011490 80.435 184 12 2 1 184 117 276 1.05e-95 285
MS.gene57443.t1 MTR_1g023370 74.093 193 14 4 1 193 117 273 9.41e-89 266
MS.gene57443.t1 MTR_5g012180 74.586 181 14 3 4 184 75 223 1.38e-85 257
MS.gene57443.t1 MTR_1g105785 45.357 280 132 3 1 280 117 375 8.99e-85 265
MS.gene57443.t1 MTR_5g045850 73.913 184 15 3 1 184 102 252 1.75e-83 253
MS.gene57443.t1 MTR_3g015660 82.857 140 9 2 1 140 117 241 3.65e-76 232
MS.gene57443.t1 MTR_8g005940 67.935 184 11 2 1 184 117 252 1.39e-73 227
MS.gene57443.t1 MTR_3g036220 66.851 181 14 2 4 184 75 209 4.71e-73 224
MS.gene57443.t1 MTR_7g110100 41.516 277 141 3 4 280 120 375 2.97e-71 228
MS.gene57443.t1 MTR_0105s0080 81.618 136 10 2 1 136 73 193 3.64e-71 219
MS.gene57443.t1 MTR_0048s0080 44.444 243 114 4 36 278 6 227 8.91e-65 206
MS.gene57443.t1 MTR_1g082860 77.273 132 6 2 1 132 117 224 3.28e-63 200
MS.gene57443.t1 MTR_2g019040 69.343 137 31 2 48 184 4 129 3.22e-59 189
MS.gene57443.t1 MTR_7g051400 37.143 280 133 5 1 280 117 353 2.67e-57 192
MS.gene57443.t1 MTR_3g449160 79.808 104 9 1 4 107 22 113 1.93e-53 169
MS.gene57443.t1 MTR_5g071020 56.354 181 12 3 4 184 75 188 3.11e-53 173
MS.gene57443.t1 MTR_2g435900 79.808 104 9 1 4 107 22 113 2.24e-52 167
MS.gene57443.t1 MTR_3g452170 37.759 241 129 3 4 244 120 339 8.80e-52 176
MS.gene57443.t1 MTR_1g052715 46.980 149 76 1 36 184 100 245 3.26e-45 155
MS.gene57443.t1 MTR_4g060433 58.678 121 44 1 156 276 1 115 1.44e-41 142
MS.gene57443.t1 MTR_8g104590 61.682 107 32 2 173 279 7 104 2.02e-40 139
MS.gene57443.t1 MTR_5g019960 49.275 138 54 3 1 138 124 245 6.47e-37 131
MS.gene57443.t1 MTR_6g024030 55.140 107 45 1 35 141 59 162 2.01e-35 127
MS.gene57443.t1 MTR_1g492690 39.459 185 82 4 90 274 8 162 2.14e-35 130
MS.gene57443.t1 MTR_4g032565 46.667 120 58 1 163 282 8 121 1.58e-32 121
MS.gene57443.t1 MTR_7g045450 40.936 171 71 2 2 172 118 258 2.43e-32 120
MS.gene57443.t1 MTR_6g073570 40.936 171 71 2 2 172 118 258 2.43e-32 120
MS.gene57443.t1 MTR_4g032585 45.833 120 59 1 163 282 8 121 8.72e-32 119
MS.gene57443.t1 MTR_3g007260 51.852 108 39 2 4 111 80 174 2.84e-31 115
MS.gene57443.t1 MTR_3g068135 49.533 107 39 1 142 248 39 130 9.78e-27 103
MS.gene57443.t1 MTR_2g450060 26.740 273 171 9 5 270 121 371 5.81e-24 101
MS.gene57443.t1 MTR_5g004860 26.523 279 178 7 4 276 116 373 1.03e-22 97.8
MS.gene57443.t1 MTR_3g055660 26.429 280 176 7 4 276 145 401 1.59e-22 97.4
MS.gene57443.t1 MTR_4g014890 26.429 280 176 7 4 276 145 401 1.65e-22 97.1
MS.gene57443.t1 MTR_5g041170 26.829 287 175 8 2 276 17 280 4.62e-22 95.5
MS.gene57443.t1 MTR_6g059860 25.714 280 178 7 4 276 139 395 9.11e-22 95.1
MS.gene57443.t1 MTR_1g110330 67.123 73 5 1 112 184 116 169 1.74e-21 90.1
MS.gene57443.t1 MTR_8g062030 26.230 244 166 7 36 277 141 372 5.27e-20 90.1
MS.gene57443.t1 MTR_7g450880 24.638 276 177 8 3 270 19 271 3.22e-19 85.9
MS.gene57443.t1 MTR_3g056270 26.721 247 160 9 36 277 300 530 3.86e-19 87.4
MS.gene57443.t1 MTR_4g036240 26.316 247 161 9 36 277 61 291 7.46e-19 86.3
MS.gene57443.t1 MTR_2g048560 24.590 244 171 6 36 277 141 373 7.63e-19 86.7
MS.gene57443.t1 MTR_4g036240 26.316 247 161 9 36 277 141 371 1.05e-18 86.3
MS.gene57443.t1 MTR_5g057125 34.091 176 93 4 100 275 1 153 2.05e-18 83.6
MS.gene57443.t1 MTR_3g046650 37.143 105 60 1 142 246 78 176 4.96e-18 81.6
MS.gene57443.t1 MTR_6g055250 29.536 237 139 7 2 232 75 289 1.78e-17 82.0
MS.gene57443.t1 MTR_0003s0290 24.812 266 177 8 3 264 83 329 9.93e-17 80.1
MS.gene57443.t1 MTR_1g043690 27.542 236 138 7 4 232 123 332 1.15e-16 80.1
MS.gene57443.t1 MTR_5g078250 24.359 234 162 8 47 278 152 372 3.11e-16 79.0
MS.gene57443.t1 MTR_6g464080 21.818 220 158 3 55 270 156 365 4.86e-15 75.1
MS.gene57443.t1 MTR_6g445520 24.696 247 171 7 37 277 1 238 1.82e-14 73.2
MS.gene57443.t1 MTR_5g053590 26.786 280 182 9 4 276 117 380 2.51e-14 73.2
MS.gene57443.t1 MTR_7g039300 48.438 64 33 0 109 172 184 247 2.24e-13 68.6
MS.gene57443.t1 MTR_4g085760 24.074 270 175 8 1 262 120 367 6.65e-13 68.9
MS.gene57443.t1 MTR_7g096440 27.273 110 68 1 2 111 71 168 1.83e-11 63.5
MS.gene57443.t1 MTR_1g075850 22.936 218 153 8 36 251 135 339 7.33e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57443.t1 AT1G52950 26.408 284 178 8 9 282 123 385 1.20e-20 92.0

Find 61 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTTGCATTGCTCTCTTT+GGG 0.202399 2.2:-50606222 MS.gene57443:CDS
ACCACTTGCATTGCTCTCTT+TGG 0.221388 2.2:-50606223 MS.gene57443:CDS
GGAGTTACTAATGCCTTTAA+TGG 0.255066 2.2:-50606001 MS.gene57443:CDS
CGCATGTATTTAGGTATATC+AGG 0.257069 2.2:-50606113 MS.gene57443:intron
TGCAAGACACGCATGTATTT+AGG 0.278275 2.2:-50606122 MS.gene57443:CDS
GCACATTACGCCCAATAAAC+TGG 0.284869 2.2:+50605047 None:intergenic
CGCCAGGTGACCCAGTTTAT+TGG 0.287098 2.2:-50605058 MS.gene57443:CDS
TATCAGCTTAGTCCCATTAA+AGG 0.310808 2.2:+50605988 None:intergenic
TAATTGAAAGAGGTGATATA+AGG 0.328150 2.2:-50606369 MS.gene57443:CDS
GGGACTAAGCTGATACTTAA+TGG 0.330761 2.2:-50605980 MS.gene57443:CDS
TACGTAATTTCAGATGTTAT+TGG 0.335367 2.2:-50606397 MS.gene57443:intron
GTCATGTACGCAGCGACTTC+AGG 0.337912 2.2:+50605950 None:intergenic
ACTATATGTGAGATCGAAAC+TGG 0.342217 2.2:-50605328 MS.gene57443:CDS
GGTTATTGATCTCACTTTAG+AGG 0.347534 2.2:-50606317 MS.gene57443:CDS
TGAAATCTGGCTAACACTTC+GGG 0.348721 2.2:+50605555 None:intergenic
GCCAGGTGACCCAGTTTATT+GGG 0.358263 2.2:-50605057 MS.gene57443:CDS
GAGTTACTAATGCCTTTAAT+GGG 0.405739 2.2:-50606000 MS.gene57443:CDS
CGGAGCTGTTCGTTGAAATC+TGG 0.418086 2.2:+50605542 None:intergenic
GAAGAGAGAATTTCATTGAA+TGG 0.432380 2.2:+50606549 None:intergenic
ATGCACCCAGTGTGATTCAT+TGG 0.435885 2.2:-50605284 MS.gene57443:CDS
TCATGCTTTCAATTGTCATT+CGG 0.442768 2.2:+50605488 None:intergenic
TTGAAATCTGGCTAACACTT+CGG 0.453680 2.2:+50605554 None:intergenic
AACTGTCGAGGAAGTGTCTA+CGG 0.454569 2.2:-50606526 MS.gene57443:CDS
CACTTGCATTGCTCTCTTTG+GGG 0.462664 2.2:-50606221 MS.gene57443:CDS
TTTACATATCTGTCAAGGTT+CGG 0.482132 2.2:-50605359 MS.gene57443:CDS
AATTGAAAGAGGTGATATAA+GGG 0.489125 2.2:-50606368 MS.gene57443:CDS
AAAAGGATGGAAGGAAAAGC+AGG 0.493666 2.2:-50606339 MS.gene57443:CDS
GCCCAATAAACTGGGTCACC+TGG 0.499906 2.2:+50605056 None:intergenic
GAAGTCGCTGCGTACATGAC+AGG 0.504902 2.2:-50605947 MS.gene57443:CDS
CACATTACGCCCAATAAACT+GGG 0.508234 2.2:+50605048 None:intergenic
AACTGCAATATCAATATAGT+AGG 0.513997 2.2:+50606146 None:intergenic
AGATCGAAACTGGGGTTGAT+TGG 0.522728 2.2:-50605318 MS.gene57443:CDS
TGATATAAGGGAAACTGAAA+AGG 0.524272 2.2:-50606356 MS.gene57443:CDS
AGTTTCGATCTCACATATAG+TGG 0.527956 2.2:+50605330 None:intergenic
ATAAGGGAAACTGAAAAGGA+TGG 0.532230 2.2:-50606352 MS.gene57443:CDS
ATTGGTCATGTAATTGAAAG+AGG 0.535259 2.2:-50606379 MS.gene57443:CDS
CAAGCTGCATTTCATCGTGA+TGG 0.555366 2.2:-50605116 MS.gene57443:CDS
CGCAAGTGTAAAACTTGCAA+GGG 0.557114 2.2:-50605232 MS.gene57443:CDS
TCGCAAGTGTAAAACTTGCA+AGG 0.564821 2.2:-50605233 MS.gene57443:CDS
CTATATGTGAGATCGAAACT+GGG 0.565040 2.2:-50605327 MS.gene57443:CDS
TCATTGGCCACCGTTGACAA+TGG 0.565967 2.2:-50605268 MS.gene57443:CDS
ACTGTCGAGGAAGTGTCTAC+GGG 0.577481 2.2:-50606525 MS.gene57443:CDS
GGGAAACTGAAAAGGATGGA+AGG 0.581802 2.2:-50606348 MS.gene57443:CDS
AAATATTTACATATCTGTCA+AGG 0.584108 2.2:-50605364 MS.gene57443:CDS
CGGTGGCCAATGAATCACAC+TGG 0.587163 2.2:+50605278 None:intergenic
TAGTAGGTGTGTGGTTGTCA+TGG 0.596523 2.2:+50606162 None:intergenic
AATTCTCTCTTCAACTGTCG+AGG 0.602150 2.2:-50606538 MS.gene57443:CDS
CTTGCAAGGGTGCAGTCCCA+AGG 0.605354 2.2:-50605219 MS.gene57443:intron
AAGTCGCTGCGTACATGACA+GGG 0.606137 2.2:-50605946 MS.gene57443:intron
TTCATCGTGATGGACGACAC+AGG 0.609546 2.2:-50605106 MS.gene57443:CDS
CTTGATCAATGAACGTGATG+AGG 0.616661 2.2:-50605020 None:intergenic
CCCAAAGAGAGCAATGCAAG+TGG 0.621311 2.2:+50606222 None:intergenic
TCATCGTGATGGACGACACA+GGG 0.635192 2.2:-50605105 MS.gene57443:CDS
ACGGAGCACTCCATTGTCAA+CGG 0.655947 2.2:+50605258 None:intergenic
GGTGGCCAATGAATCACACT+GGG 0.672724 2.2:+50605279 None:intergenic
CAAGTCATCTGATAAAGACG+CGG 0.674931 2.2:+50605522 None:intergenic
AAAGGATGGAAGGAAAAGCA+GGG 0.682942 2.2:-50606338 MS.gene57443:CDS
GAGCACTCCATTGTCAACGG+TGG 0.720804 2.2:+50605261 None:intergenic
TATATGTGAGATCGAAACTG+GGG 0.729438 2.2:-50605326 MS.gene57443:CDS
ATATCAATATAGTAGGTGTG+TGG 0.756687 2.2:+50606153 None:intergenic
AAGCATGATTGAGTCAACTG+AGG 0.787148 2.2:-50605473 MS.gene57443:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAACATATAAAAATGTAAA+TGG + chr2.2:50605674-50605693 None:intergenic 10.0%
!!! TTTTATATGTTATAGAATAA+AGG - chr2.2:50605681-50605700 MS.gene57443:intron 10.0%
!! ATTGTCCAATTATTATTAAT+AGG - chr2.2:50605904-50605923 MS.gene57443:intron 15.0%
!! TGTAACCTATTAATAATAAT+TGG + chr2.2:50605912-50605931 None:intergenic 15.0%
!!! CATTTTTCATTCTAATAAAA+GGG + chr2.2:50606001-50606020 None:intergenic 15.0%
!!! GAAATTTTTAGATGTATTTT+TGG - chr2.2:50606157-50606176 MS.gene57443:CDS 15.0%
!!! TGTTGAATTTTTGAAAAATT+AGG - chr2.2:50605560-50605579 MS.gene57443:CDS 15.0%
!! AAATATTTACATATCTGTCA+AGG - chr2.2:50606227-50606246 MS.gene57443:CDS 20.0%
!! GATATAGTTGATTATTTGAT+AGG - chr2.2:50606450-50606469 MS.gene57443:intron 20.0%
!! TATTACCAAAATATCAATTC+AGG + chr2.2:50605332-50605351 None:intergenic 20.0%
!!! ATTTTTGTTTTTATCATGTG+TGG - chr2.2:50606418-50606437 MS.gene57443:intron 20.0%
!!! GCATTTTTCATTCTAATAAA+AGG + chr2.2:50606002-50606021 None:intergenic 20.0%
!!! GTTCAATATTTTTATGCAAA+AGG - chr2.2:50606199-50606218 MS.gene57443:CDS 20.0%
! AACTGCAATATCAATATAGT+AGG + chr2.2:50605448-50605467 None:intergenic 25.0%
! AATGTGAAATTATGTTCACT+TGG - chr2.2:50605537-50605556 MS.gene57443:CDS 25.0%
! AATTGAAAGAGGTGATATAA+GGG - chr2.2:50605223-50605242 MS.gene57443:CDS 25.0%
! ATCAAAAAGAACAAAAGTAG+TGG + chr2.2:50606513-50606532 None:intergenic 25.0%
! CAAATGTATGAAATTCCTAA+AGG + chr2.2:50605153-50605172 None:intergenic 25.0%
! TAATTGAAAGAGGTGATATA+AGG - chr2.2:50605222-50605241 MS.gene57443:CDS 25.0%
! TACGTAATTTCAGATGTTAT+TGG - chr2.2:50605194-50605213 MS.gene57443:intron 25.0%
!! ATGTTCCTGAATTGATATTT+TGG - chr2.2:50605324-50605343 MS.gene57443:CDS 25.0%
!! ATTGTTAGTGGATATCATTA+GGG + chr2.2:50605780-50605799 None:intergenic 25.0%
!! TATTGTTAGTGGATATCATT+AGG + chr2.2:50605781-50605800 None:intergenic 25.0%
!! TTTGAAAAATTAGGTGCTAT+GGG - chr2.2:50605569-50605588 MS.gene57443:CDS 25.0%
!!! AGAATAAAGGCAAGTTTTTT+TGG - chr2.2:50605694-50605713 MS.gene57443:intron 25.0%
!!! TTTTGAAAAATTAGGTGCTA+TGG - chr2.2:50605568-50605587 MS.gene57443:CDS 25.0%
ATATCAATATAGTAGGTGTG+TGG + chr2.2:50605441-50605460 None:intergenic 30.0%
ATTCCTAAAGGAATGCTTAT+AGG + chr2.2:50605141-50605160 None:intergenic 30.0%
ATTGGTCATGTAATTGAAAG+AGG - chr2.2:50605212-50605231 MS.gene57443:intron 30.0%
CATCAGAAAACTATTGTTAG+TGG + chr2.2:50605792-50605811 None:intergenic 30.0%
GAAGAGAGAATTTCATTGAA+TGG + chr2.2:50605045-50605064 None:intergenic 30.0%
GAGTTACTAATGCCTTTAAT+GGG - chr2.2:50605591-50605610 MS.gene57443:intron 30.0%
TCATGCTTTCAATTGTCATT+CGG + chr2.2:50606106-50606125 None:intergenic 30.0%
TGATATAAGGGAAACTGAAA+AGG - chr2.2:50605235-50605254 MS.gene57443:CDS 30.0%
TTTACATATCTGTCAAGGTT+CGG - chr2.2:50606232-50606251 MS.gene57443:intron 30.0%
! CACTAACAATAGTTTTCTGA+TGG - chr2.2:50605790-50605809 MS.gene57443:intron 30.0%
! GGATAAACTTTGTTTACCTT+GGG + chr2.2:50606391-50606410 None:intergenic 30.0%
! GTCATAATTAAGTTGTCTAG+AGG - chr2.2:50605716-50605735 MS.gene57443:intron 30.0%
!!! GACAAAATTGTGACCTTTTT+TGG - chr2.2:50605403-50605422 MS.gene57443:intron 30.0%
AATAAGCTACCCATAAACGA+TGG + chr2.2:50605747-50605766 None:intergenic 35.0%
ACTATATGTGAGATCGAAAC+TGG - chr2.2:50606263-50606282 MS.gene57443:intron 35.0%
AGAAGATGTGCACAAAGAAA+AGG - chr2.2:50605846-50605865 MS.gene57443:intron 35.0%
AGTTTCGATCTCACATATAG+TGG + chr2.2:50606264-50606283 None:intergenic 35.0%
ATAAGCTACCCATAAACGAT+GGG + chr2.2:50605746-50605765 None:intergenic 35.0%
ATAAGGGAAACTGAAAAGGA+TGG - chr2.2:50605239-50605258 MS.gene57443:CDS 35.0%
CGCATGTATTTAGGTATATC+AGG - chr2.2:50605478-50605497 MS.gene57443:CDS 35.0%
CTACCTATAAGCATTCCTTT+AGG - chr2.2:50605135-50605154 MS.gene57443:intron 35.0%
CTATATGTGAGATCGAAACT+GGG - chr2.2:50606264-50606283 MS.gene57443:intron 35.0%
GAAGATGTGCACAAAGAAAA+GGG - chr2.2:50605847-50605866 MS.gene57443:intron 35.0%
GGAGTTACTAATGCCTTTAA+TGG - chr2.2:50605590-50605609 MS.gene57443:intron 35.0%
GGTTATTGATCTCACTTTAG+AGG - chr2.2:50605274-50605293 MS.gene57443:CDS 35.0%
TATATGTGAGATCGAAACTG+GGG - chr2.2:50606265-50606284 MS.gene57443:intron 35.0%
TATCAGCTTAGTCCCATTAA+AGG + chr2.2:50605606-50605625 None:intergenic 35.0%
TCGTTTATGGGTAGCTTATT+TGG - chr2.2:50605747-50605766 MS.gene57443:intron 35.0%
TTGAAATCTGGCTAACACTT+CGG + chr2.2:50606040-50606059 None:intergenic 35.0%
! AAGTTTTACACTTGCGACAA+CGG + chr2.2:50606355-50606374 None:intergenic 35.0%
! CGGATAAACTTTGTTTACCT+TGG + chr2.2:50606392-50606411 None:intergenic 35.0%
! TTTTGTTCTTTTTGATCGCC+AGG - chr2.2:50606517-50606536 MS.gene57443:CDS 35.0%
AAAAGGATGGAAGGAAAAGC+AGG - chr2.2:50605252-50605271 MS.gene57443:CDS 40.0%
AAAGGATGGAAGGAAAAGCA+GGG - chr2.2:50605253-50605272 MS.gene57443:CDS 40.0%
AAGCATGATTGAGTCAACTG+AGG - chr2.2:50606118-50606137 MS.gene57443:CDS 40.0%
AATTCTCTCTTCAACTGTCG+AGG - chr2.2:50605053-50605072 MS.gene57443:CDS 40.0%
AGACTCAAGTAGCAAGGTAA+GGG + chr2.2:50605953-50605972 None:intergenic 40.0%
CAAGTCATCTGATAAAGACG+CGG + chr2.2:50606072-50606091 None:intergenic 40.0%
CACATTACGCCCAATAAACT+GGG + chr2.2:50606546-50606565 None:intergenic 40.0%
CGCAAGTGTAAAACTTGCAA+GGG - chr2.2:50606359-50606378 MS.gene57443:CDS 40.0%
CGCTACTCTTCTATTGACAT+TGG - chr2.2:50605973-50605992 MS.gene57443:CDS 40.0%
GGGACTAAGCTGATACTTAA+TGG - chr2.2:50605611-50605630 MS.gene57443:intron 40.0%
GTTGTCATGGTTGCCAAAAA+AGG + chr2.2:50605419-50605438 None:intergenic 40.0%
TCGCAAGTGTAAAACTTGCA+AGG - chr2.2:50606358-50606377 MS.gene57443:CDS 40.0%
TGAAATCTGGCTAACACTTC+GGG + chr2.2:50606039-50606058 None:intergenic 40.0%
TGCAAGACACGCATGTATTT+AGG - chr2.2:50605469-50605488 MS.gene57443:intron 40.0%
ACCACTTGCATTGCTCTCTT+TGG - chr2.2:50605368-50605387 MS.gene57443:CDS 45.0%
ATGCACCCAGTGTGATTCAT+TGG - chr2.2:50606307-50606326 MS.gene57443:intron 45.0%
CAAGCTGCATTTCATCGTGA+TGG - chr2.2:50606475-50606494 MS.gene57443:intron 45.0%
CACTTGCATTGCTCTCTTTG+GGG - chr2.2:50605370-50605389 MS.gene57443:CDS 45.0%
CATGATTGAGTCAACTGAGG+TGG - chr2.2:50606121-50606140 MS.gene57443:CDS 45.0%
CCACTTGCATTGCTCTCTTT+GGG - chr2.2:50605369-50605388 MS.gene57443:CDS 45.0%
GAGACTCAAGTAGCAAGGTA+AGG + chr2.2:50605954-50605973 None:intergenic 45.0%
GCACATTACGCCCAATAAAC+TGG + chr2.2:50606547-50606566 None:intergenic 45.0%
GGGAAACTGAAAAGGATGGA+AGG - chr2.2:50605243-50605262 MS.gene57443:CDS 45.0%
TAGTAGGTGTGTGGTTGTCA+TGG + chr2.2:50605432-50605451 None:intergenic 45.0%
! AGATCGAAACTGGGGTTGAT+TGG - chr2.2:50606273-50606292 MS.gene57443:intron 45.0%
!! AACTGTCGAGGAAGTGTCTA+CGG - chr2.2:50605065-50605084 MS.gene57443:CDS 45.0%
!! ATAAAAACAAAAATTATATT+CGG + chr2.2:50606412-50606431 None:intergenic 5.0%
AAGTCGCTGCGTACATGACA+GGG - chr2.2:50605645-50605664 MS.gene57443:intron 50.0%
AGAGGTCCTCCCATCGTTTA+TGG - chr2.2:50605734-50605753 MS.gene57443:intron 50.0%
AGCTACCCATAAACGATGGG+AGG + chr2.2:50605743-50605762 None:intergenic 50.0%
CCCAAAGAGAGCAATGCAAG+TGG + chr2.2:50605372-50605391 None:intergenic 50.0%
CGGAGCTGTTCGTTGAAATC+TGG + chr2.2:50606052-50606071 None:intergenic 50.0%
GAGGTCCTCCCATCGTTTAT+GGG - chr2.2:50605735-50605754 MS.gene57443:intron 50.0%
GCCAGGTGACCCAGTTTATT+GGG - chr2.2:50606534-50606553 MS.gene57443:CDS 50.0%
GGTGGCCAATGAATCACACT+GGG + chr2.2:50606315-50606334 None:intergenic 50.0%
GTAGCGAGACTCAAGTAGCA+AGG + chr2.2:50605959-50605978 None:intergenic 50.0%
TCATTGGCCACCGTTGACAA+TGG - chr2.2:50606323-50606342 MS.gene57443:CDS 50.0%
! ACGGAGCACTCCATTGTCAA+CGG + chr2.2:50606336-50606355 None:intergenic 50.0%
!! ACTGTCGAGGAAGTGTCTAC+GGG - chr2.2:50605066-50605085 MS.gene57443:CDS 50.0%
!! TCATCGTGATGGACGACACA+GGG - chr2.2:50606486-50606505 MS.gene57443:intron 50.0%
!! TTCATCGTGATGGACGACAC+AGG - chr2.2:50606485-50606504 MS.gene57443:intron 50.0%
CGCCAGGTGACCCAGTTTAT+TGG - chr2.2:50606533-50606552 MS.gene57443:CDS 55.0%
CGGTGGCCAATGAATCACAC+TGG + chr2.2:50606316-50606335 None:intergenic 55.0%
GAAGTCGCTGCGTACATGAC+AGG - chr2.2:50605644-50605663 MS.gene57443:intron 55.0%
GAGCACTCCATTGTCAACGG+TGG + chr2.2:50606333-50606352 None:intergenic 55.0%
GCCCAATAAACTGGGTCACC+TGG + chr2.2:50606538-50606557 None:intergenic 55.0%
GTCATGTACGCAGCGACTTC+AGG + chr2.2:50605644-50605663 None:intergenic 55.0%
! CTTGCAAGGGTGCAGTCCCA+AGG - chr2.2:50606372-50606391 MS.gene57443:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 50605021 50606592 50605021 ID=MS.gene57443
chr2.2 mRNA 50605021 50606592 50605021 ID=MS.gene57443.t1;Parent=MS.gene57443
chr2.2 exon 50606490 50606592 50606490 ID=MS.gene57443.t1.exon1;Parent=MS.gene57443.t1
chr2.2 CDS 50606490 50606592 50606490 ID=cds.MS.gene57443.t1;Parent=MS.gene57443.t1
chr2.2 exon 50606310 50606406 50606310 ID=MS.gene57443.t1.exon2;Parent=MS.gene57443.t1
chr2.2 CDS 50606310 50606406 50606310 ID=cds.MS.gene57443.t1;Parent=MS.gene57443.t1
chr2.2 exon 50606114 50606250 50606114 ID=MS.gene57443.t1.exon3;Parent=MS.gene57443.t1
chr2.2 CDS 50606114 50606250 50606114 ID=cds.MS.gene57443.t1;Parent=MS.gene57443.t1
chr2.2 exon 50605947 50606031 50605947 ID=MS.gene57443.t1.exon4;Parent=MS.gene57443.t1
chr2.2 CDS 50605947 50606031 50605947 ID=cds.MS.gene57443.t1;Parent=MS.gene57443.t1
chr2.2 exon 50605474 50605603 50605474 ID=MS.gene57443.t1.exon5;Parent=MS.gene57443.t1
chr2.2 CDS 50605474 50605603 50605474 ID=cds.MS.gene57443.t1;Parent=MS.gene57443.t1
chr2.2 exon 50605220 50605392 50605220 ID=MS.gene57443.t1.exon6;Parent=MS.gene57443.t1
chr2.2 CDS 50605220 50605392 50605220 ID=cds.MS.gene57443.t1;Parent=MS.gene57443.t1
chr2.2 exon 50605021 50605141 50605021 ID=MS.gene57443.t1.exon7;Parent=MS.gene57443.t1
chr2.2 CDS 50605021 50605141 50605021 ID=cds.MS.gene57443.t1;Parent=MS.gene57443.t1
Gene Sequence

>MS.gene57443

ATGAACGTTTTTGAGTTCAAGCCATTCAATGAAATTCTCTCTTCAACTGTCGAGGAAGTGTCTACGGGTAAGACTTCAAAGACTTCAATTCTGAATGTGCGTTGTACATTATTACTACCTATAAGCATTCCTTTAGGAATTTCATACATTTGTTATATGTTTTTGCTTTTGTATACGTAATTTCAGATGTTATTGGTCATGTAATTGAAAGAGGTGATATAAGGGAAACTGAAAAGGATGGAAGGAAAAGCAGGGTTATTGATCTCACTTTAGAGGATCTTGAGTAAGCTTTGTCTGTTTCTTATGTTCCTGAATTGATATTTTGGTAATATTTTATTACAGAAACAACCACTTGCATTGCTCTCTTTGGGGTGAACATGCTGACAAAATTGTGACCTTTTTTGGCAACCATGACAACCACACACCTACTATATTGATATTGCAGTTCTGCAAGACACGCATGTATTTAGGTATATCAGGTTTTAATTTTGTGCTTTCTGCTGATATCAAGAATGAAATGTGAAATTATGTTCACTTGGTGTTGAATTTTTGAAAAATTAGGTGCTATGGGAGTTACTAATGCCTTTAATGGGACTAAGCTGATACTTAATGGCGATTTACCTGAAGTCGCTGCGTACATGACAGGGTAACCATTTACATTTTTATATGTTATAGAATAAAGGCAAGTTTTTTTGGTCATAATTAAGTTGTCTAGAGGTCCTCCCATCGTTTATGGGTAGCTTATTTGGCATACGACCCTAATGATATCCACTAACAATAGTTTTCTGATGGCATGTTAGAATTTGTAATAAGTAAGAAAGTTTGAGAAGATGTGCACAAAGAAAAGGGCAGCACAATATTAATAGTAACACTGTCAAATATTATTGTCCAATTATTATTAATAGGTTACATGAGTTATATGTTGTCGTCCCTTACCTTGCTACTTGAGTCTCGCTACTCTTCTATTGACATTGGTGCCCTTTTATTAGAATGAAAAATGCATCAATACAGTTAACCCGAAGTGTTAGCCAGATTTCAACGAACAGCTCCGCGTCTTTATCAGATGACTTGCTCAACATCAACCGAATGACAATTGAAAGCATGATTGAGTCAACTGAGGTGGCTATCTGTTTAATGAAATTTTTAGATGTATTTTTGGATTCATCTTTGTTATTTTTGTTCAATATTTTTATGCAAAAGGCTACAAAATATTTACATATCTGTCAAGGTTCGGTGCTTGCCACTATATGTGAGATCGAAACTGGGGTTGATTGGTACTTTCGATCATGCACCCAGTGTGATTCATTGGCCACCGTTGACAATGGAGTGCTCCGTTGTCGCAAGTGTAAAACTTGCAAGGGTGCAGTCCCAAGGTAAACAAAGTTTATCCGAATATAATTTTTGTTTTTATCATGTGTGGAATTGTATTGATATAGTTGATTATTTGATAGGTTCAAGCTGCATTTCATCGTGATGGACGACACAGGGTCCACTACTTTTGTTCTTTTTGATCGCCAGGTGACCCAGTTTATTGGGCGTAATGTGCAAGACTTGATCAATGAACGTGATGAG
Protein sequence

>MS.gene57443.t1

MNVFEFKPFNEILSSTVEEVSTGKTSKTSILNVRYVIGHVIERGDIRETEKDGRKSRVIDLTLEDLENNHLHCSLWGEHADKIVTFFGNHDNHTPTILILQFCKTRMYLGISGAMGVTNAFNGTKLILNGDLPEVAAYMTGMKNASIQLTRSVSQISTNSSASLSDDLLNINRMTIESMIESTEATKYLHICQGSVLATICEIETGVDWYFRSCTQCDSLATVDNGVLRCRKCKTCKGAVPRFKLHFIVMDDTGSTTFVLFDRQVTQFIGRNVQDLINERDE