Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57497.t1 | PNY02171.1 | 49.4 | 267 | 97 | 5 | 6 | 237 | 51 | 314 | 1.80E-50 | 209.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57497.t1 | Q9FHE1 | 34.7 | 219 | 111 | 7 | 15 | 206 | 246 | 459 | 4.4e-21 | 103.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57497.t1 | A0A2K3NGK5 | 49.4 | 267 | 97 | 5 | 6 | 237 | 51 | 314 | 1.3e-50 | 209.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene57497 | MS.gene90825 | PPI |
| MS.gene57497 | MS.gene97346 | PPI |
| MS.gene57497 | MS.gene029093 | PPI |
| MS.gene57497 | MS.gene066439 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57497.t1 | MTR_7g088160 | 34.909 | 275 | 133 | 4 | 13 | 244 | 76 | 347 | 4.88e-50 | 169 |
| MS.gene57497.t1 | MTR_4g063670 | 43.868 | 212 | 91 | 4 | 4 | 193 | 9 | 214 | 8.49e-49 | 164 |
| MS.gene57497.t1 | MTR_0867s0010 | 45.327 | 214 | 90 | 5 | 1 | 193 | 7 | 214 | 2.09e-44 | 152 |
| MS.gene57497.t1 | MTR_7g107340 | 38.806 | 268 | 113 | 5 | 2 | 250 | 68 | 303 | 9.77e-42 | 145 |
| MS.gene57497.t1 | MTR_4g083510 | 42.308 | 208 | 90 | 6 | 8 | 193 | 16 | 215 | 2.79e-38 | 136 |
| MS.gene57497.t1 | MTR_7g114170 | 36.400 | 250 | 117 | 3 | 1 | 250 | 52 | 259 | 4.10e-33 | 122 |
| MS.gene57497.t1 | MTR_1g051185 | 45.509 | 167 | 66 | 4 | 62 | 209 | 45 | 205 | 1.89e-31 | 117 |
| MS.gene57497.t1 | MTR_3g069460 | 39.157 | 166 | 80 | 3 | 1 | 145 | 7 | 172 | 2.12e-30 | 112 |
| MS.gene57497.t1 | MTR_6g081150 | 35.185 | 216 | 87 | 5 | 3 | 218 | 8 | 170 | 3.73e-28 | 106 |
| MS.gene57497.t1 | MTR_8g015740 | 48.684 | 76 | 37 | 1 | 1 | 74 | 55 | 130 | 4.35e-13 | 65.1 |
| MS.gene57497.t1 | MTR_4g026120 | 25.714 | 210 | 113 | 5 | 12 | 196 | 53 | 244 | 1.54e-11 | 63.9 |
| MS.gene57497.t1 | MTR_4g036435 | 25.714 | 210 | 113 | 5 | 12 | 196 | 53 | 244 | 1.54e-11 | 63.9 |
| MS.gene57497.t1 | MTR_3g067785 | 25.714 | 210 | 113 | 5 | 12 | 196 | 53 | 244 | 1.68e-11 | 63.5 |
| MS.gene57497.t1 | MTR_5g066280 | 25.714 | 210 | 113 | 5 | 12 | 196 | 53 | 244 | 1.85e-11 | 63.5 |
| MS.gene57497.t1 | MTR_4g013220 | 25.714 | 210 | 113 | 5 | 12 | 196 | 53 | 244 | 2.64e-11 | 63.2 |
| MS.gene57497.t1 | MTR_8g015750 | 38.835 | 103 | 41 | 4 | 91 | 193 | 1 | 81 | 3.67e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57497.t1 | AT5G41220 | 33.906 | 233 | 122 | 8 | 1 | 206 | 174 | 401 | 1.55e-26 | 107 |
| MS.gene57497.t1 | AT5G41220 | 33.906 | 233 | 122 | 8 | 1 | 206 | 182 | 409 | 2.28e-26 | 107 |
| MS.gene57497.t1 | AT5G41220 | 33.906 | 233 | 122 | 8 | 1 | 206 | 232 | 459 | 2.31e-26 | 107 |
| MS.gene57497.t1 | AT5G41240 | 36.139 | 202 | 97 | 8 | 31 | 204 | 261 | 458 | 7.40e-24 | 100 |
| MS.gene57497.t1 | AT3G47680 | 33.083 | 133 | 69 | 2 | 32 | 145 | 38 | 169 | 2.63e-16 | 77.0 |
Find 49 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGCAATTCTCTACTCAAAT+TGG | 0.240436 | 8.2:+65220890 | MS.gene57497:CDS |
| AATTGCATCTCCCTTCCATT+TGG | 0.301545 | 8.2:-65221278 | None:intergenic |
| ATTCTCACTCTCCCTTGCTT+TGG | 0.319862 | 8.2:-65221563 | None:intergenic |
| ATTGCATCTCCCTTCCATTT+GGG | 0.325205 | 8.2:-65221277 | None:intergenic |
| ATTCTCTACTCAAATTGGTT+TGG | 0.327637 | 8.2:+65220895 | MS.gene57497:CDS |
| GAATTGCGACATGTCGTTAC+CGG | 0.373955 | 8.2:-65220876 | None:intergenic |
| GAACATTCAATAGGTGTTCC+TGG | 0.375216 | 8.2:-65221361 | None:intergenic |
| TCTAGCGGTGGTGTGGATTC+CGG | 0.384319 | 8.2:+65220857 | MS.gene57497:CDS |
| ATTTCGAAGGACTCAATTGT+TGG | 0.421062 | 8.2:+65221025 | MS.gene57497:CDS |
| CCCATTGGACGGACCACTAC+TGG | 0.421593 | 8.2:-65221403 | None:intergenic |
| GAAAATATTAAGCTCGATGA+AGG | 0.423359 | 8.2:+65220917 | MS.gene57497:CDS |
| GGCCGTATTCAGAAGTTTGT+TGG | 0.432458 | 8.2:+65221115 | MS.gene57497:CDS |
| AATTCCTTCATGTCCATGCT+TGG | 0.434472 | 8.2:+65221236 | MS.gene57497:CDS |
| ATAGAAATGTTATCATGTTA+AGG | 0.443110 | 8.2:+65221515 | MS.gene57497:CDS |
| TAATGTTGATTTATCTGTCA+TGG | 0.461525 | 8.2:+65221477 | MS.gene57497:CDS |
| ACACACTTCTATTTCAATCA+TGG | 0.464422 | 8.2:+65220996 | MS.gene57497:CDS |
| TACAACATCTATCGTTTGAA+TGG | 0.465935 | 8.2:+65221094 | MS.gene57497:CDS |
| GAACAAAGGTTTCTAGTAGT+GGG | 0.465990 | 8.2:+65221317 | MS.gene57497:CDS |
| CCAGTAGTGGTCCGTCCAAT+GGG | 0.470497 | 8.2:+65221403 | MS.gene57497:CDS |
| TCATAGTCTGAACATTCAAT+AGG | 0.474267 | 8.2:-65221370 | None:intergenic |
| GCTTGGATGTTATTGAAAGA+TGG | 0.488515 | 8.2:+65221253 | MS.gene57497:CDS |
| ACCAGTAGTGGTCCGTCCAA+TGG | 0.498911 | 8.2:+65221402 | MS.gene57497:CDS |
| GCCCTTAATCAAAACAAAAG+TGG | 0.500880 | 8.2:+65221151 | MS.gene57497:CDS |
| AGCCAACAAACTTCTGAATA+CGG | 0.508865 | 8.2:-65221117 | None:intergenic |
| AGAACAAAGGTTTCTAGTAG+TGG | 0.521331 | 8.2:+65221316 | MS.gene57497:CDS |
| ACGCTCTGAGAATGATGTCT+TGG | 0.531457 | 8.2:+65221174 | MS.gene57497:CDS |
| TATTGAAAGATGGACCCAAA+TGG | 0.531956 | 8.2:+65221263 | MS.gene57497:CDS |
| ACATCCAAGCATGGACATGA+AGG | 0.537433 | 8.2:-65221240 | None:intergenic |
| AGAGTGAGAATGAGACAAAG+TGG | 0.548397 | 8.2:+65221575 | MS.gene57497:CDS |
| AGAAAGAGAGTCCAAAGCAA+GGG | 0.551858 | 8.2:+65221552 | MS.gene57497:CDS |
| GAAAGATGGACCCAAATGGA+AGG | 0.554240 | 8.2:+65221267 | MS.gene57497:CDS |
| TACACAACATCTTCAAATCC+AGG | 0.559189 | 8.2:+65221343 | MS.gene57497:CDS |
| AATGGGTTCTAGCGGTGGTG+TGG | 0.575768 | 8.2:+65220850 | None:intergenic |
| TGAGAATGATGTCTTGGCTG+AGG | 0.576801 | 8.2:+65221180 | MS.gene57497:CDS |
| GAGAAAGAGAGTCCAAAGCA+AGG | 0.579565 | 8.2:+65221551 | MS.gene57497:CDS |
| GGACGGACCACTACTGGTGA+TGG | 0.588149 | 8.2:-65221397 | None:intergenic |
| ATGTCTTGGCTGAGGCACAT+AGG | 0.590198 | 8.2:+65221188 | MS.gene57497:CDS |
| ATCATGGCTCAACATTTCGA+AGG | 0.593264 | 8.2:+65221012 | MS.gene57497:CDS |
| CCTCCAATGGGTTCTAGCGG+TGG | 0.596036 | 8.2:+65220845 | None:intergenic |
| ACAAAGGTTTCTAGTAGTGG+GGG | 0.603594 | 8.2:+65221319 | MS.gene57497:CDS |
| AAATGTTATCATGTTAAGGA+TGG | 0.603681 | 8.2:+65221519 | MS.gene57497:CDS |
| ACACCACCGCTAGAACCCAT+TGG | 0.607694 | 8.2:-65220848 | None:intergenic |
| AAAGATGGACCCAAATGGAA+GGG | 0.610908 | 8.2:+65221268 | MS.gene57497:CDS |
| AACAAAGGTTTCTAGTAGTG+GGG | 0.611547 | 8.2:+65221318 | MS.gene57497:CDS |
| GGAGTCGACCAACAATCAGA+TGG | 0.620171 | 8.2:+65221046 | MS.gene57497:CDS |
| TGAACAGCCATCACCAGTAG+TGG | 0.622487 | 8.2:+65221390 | MS.gene57497:CDS |
| CTTTCAATAACATCCAAGCA+TGG | 0.629504 | 8.2:-65221249 | None:intergenic |
| AGGCCTCCAATGGGTTCTAG+CGG | 0.662393 | 8.2:+65220842 | None:intergenic |
| CAAAGGTTTCTAGTAGTGGG+GGG | 0.710785 | 8.2:+65221320 | MS.gene57497:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTTTTATAATGAACTTT+GGG | + | chr8.2:65221964-65221983 | MS.gene57497:intron | 10.0% |
| !!! | TTAATTTTTATAATGAACTT+TGG | + | chr8.2:65221963-65221982 | MS.gene57497:intron | 10.0% |
| !! | ATAGAAATGTTATCATGTTA+AGG | + | chr8.2:65221515-65221534 | MS.gene57497:CDS | 20.0% |
| !! | TAATATAGTGAAGCAATTAT+GGG | - | chr8.2:65222018-65222037 | None:intergenic | 20.0% |
| !! | TTAATATAGTGAAGCAATTA+TGG | - | chr8.2:65222019-65222038 | None:intergenic | 20.0% |
| !!! | AAACTTTTCAAAAAGAACAA+AGG | + | chr8.2:65221303-65221322 | MS.gene57497:CDS | 20.0% |
| ! | AAATGTTATCATGTTAAGGA+TGG | + | chr8.2:65221519-65221538 | MS.gene57497:CDS | 25.0% |
| ! | GAATTACTTACTTACTTACA+GGG | - | chr8.2:65222061-65222080 | None:intergenic | 25.0% |
| ! | TGAATTACTTACTTACTTAC+AGG | - | chr8.2:65222062-65222081 | None:intergenic | 25.0% |
| !! | ACTTACAGGGATAATATTTT+GGG | - | chr8.2:65222048-65222067 | None:intergenic | 25.0% |
| !! | TACTTACAGGGATAATATTT+TGG | - | chr8.2:65222049-65222068 | None:intergenic | 25.0% |
| !!! | ACGAGTTTTAGTTTTTTTCT+TGG | - | chr8.2:65220954-65220973 | None:intergenic | 25.0% |
| !!! | TAATGTTGATTTATCTGTCA+TGG | + | chr8.2:65221477-65221496 | MS.gene57497:CDS | 25.0% |
| !!! | TCCACTTTTGTTTTGATTAA+GGG | - | chr8.2:65221155-65221174 | None:intergenic | 25.0% |
| ACACACTTCTATTTCAATCA+TGG | + | chr8.2:65220996-65221015 | MS.gene57497:CDS | 30.0% | |
| ATTCTCTACTCAAATTGGTT+TGG | + | chr8.2:65220895-65220914 | MS.gene57497:CDS | 30.0% | |
| GAAAATATTAAGCTCGATGA+AGG | + | chr8.2:65220917-65220936 | MS.gene57497:CDS | 30.0% | |
| TACAACATCTATCGTTTGAA+TGG | + | chr8.2:65221094-65221113 | MS.gene57497:CDS | 30.0% | |
| TCATAGTCTGAACATTCAAT+AGG | - | chr8.2:65221373-65221392 | None:intergenic | 30.0% | |
| ! | ATCATGAATTTTGTTGCAGT+CGG | + | chr8.2:65221650-65221669 | MS.gene57497:intron | 30.0% |
| !!! | ACTTTTGTTTTGATTAAGGG+CGG | - | chr8.2:65221152-65221171 | None:intergenic | 30.0% |
| !!! | GAGTTGCTTTTAGTAATTCT+AGG | + | chr8.2:65222101-65222120 | MS.gene57497:intron | 30.0% |
| !!! | GTCCACTTTTGTTTTGATTA+AGG | - | chr8.2:65221156-65221175 | None:intergenic | 30.0% |
| AGCCAACAAACTTCTGAATA+CGG | - | chr8.2:65221120-65221139 | None:intergenic | 35.0% | |
| ATTTCGAAGGACTCAATTGT+TGG | + | chr8.2:65221025-65221044 | MS.gene57497:CDS | 35.0% | |
| CTTTCAATAACATCCAAGCA+TGG | - | chr8.2:65221252-65221271 | None:intergenic | 35.0% | |
| GCCCTTAATCAAAACAAAAG+TGG | + | chr8.2:65221151-65221170 | MS.gene57497:CDS | 35.0% | |
| GCTTGGATGTTATTGAAAGA+TGG | + | chr8.2:65221253-65221272 | MS.gene57497:CDS | 35.0% | |
| TACACAACATCTTCAAATCC+AGG | + | chr8.2:65221343-65221362 | MS.gene57497:CDS | 35.0% | |
| TAGTAGTTGTATCCTTGATG+AGG | - | chr8.2:65221609-65221628 | None:intergenic | 35.0% | |
| TATTGAAAGATGGACCCAAA+TGG | + | chr8.2:65221263-65221282 | MS.gene57497:CDS | 35.0% | |
| TCCAAAAACCATCTGATTGT+TGG | - | chr8.2:65221057-65221076 | None:intergenic | 35.0% | |
| TCGCAATTCTCTACTCAAAT+TGG | + | chr8.2:65220890-65220909 | MS.gene57497:CDS | 35.0% | |
| TGCTTATTGTTTCACGTTGA+AGG | + | chr8.2:65221816-65221835 | MS.gene57497:intron | 35.0% | |
| ! | AATTTTGTTGCAGTCGGATT+AGG | + | chr8.2:65221656-65221675 | MS.gene57497:intron | 35.0% |
| ! | ACCAACAATCAGATGGTTTT+TGG | + | chr8.2:65221053-65221072 | MS.gene57497:CDS | 35.0% |
| !! | AACAAAGGTTTCTAGTAGTG+GGG | + | chr8.2:65221318-65221337 | MS.gene57497:CDS | 35.0% |
| !! | AGAACAAAGGTTTCTAGTAG+TGG | + | chr8.2:65221316-65221335 | MS.gene57497:CDS | 35.0% |
| !! | GAACAAAGGTTTCTAGTAGT+GGG | + | chr8.2:65221317-65221336 | MS.gene57497:CDS | 35.0% |
| AAAGATGGACCCAAATGGAA+GGG | + | chr8.2:65221268-65221287 | MS.gene57497:CDS | 40.0% | |
| AATTCCTTCATGTCCATGCT+TGG | + | chr8.2:65221236-65221255 | MS.gene57497:CDS | 40.0% | |
| AATTGCATCTCCCTTCCATT+TGG | - | chr8.2:65221281-65221300 | None:intergenic | 40.0% | |
| AGAAAGAGAGTCCAAAGCAA+GGG | + | chr8.2:65221552-65221571 | MS.gene57497:CDS | 40.0% | |
| AGAGTGAGAATGAGACAAAG+TGG | + | chr8.2:65221575-65221594 | MS.gene57497:CDS | 40.0% | |
| ATCATGGCTCAACATTTCGA+AGG | + | chr8.2:65221012-65221031 | MS.gene57497:CDS | 40.0% | |
| ATTGCATCTCCCTTCCATTT+GGG | - | chr8.2:65221280-65221299 | None:intergenic | 40.0% | |
| GAACATTCAATAGGTGTTCC+TGG | - | chr8.2:65221364-65221383 | None:intergenic | 40.0% | |
| GTGGTATGAAATCCTCATCA+AGG | + | chr8.2:65221594-65221613 | MS.gene57497:intron | 40.0% | |
| TGGGACAAAAAGCAGCAAAA+AGG | + | chr8.2:65221422-65221441 | MS.gene57497:CDS | 40.0% | |
| !! | ACAAAGGTTTCTAGTAGTGG+GGG | + | chr8.2:65221319-65221338 | MS.gene57497:CDS | 40.0% |
| !!! | TTTGCTGCTTTTTGTCCCAT+TGG | - | chr8.2:65221421-65221440 | None:intergenic | 40.0% |
| ACATCCAAGCATGGACATGA+AGG | - | chr8.2:65221243-65221262 | None:intergenic | 45.0% | |
| ATTCTCACTCTCCCTTGCTT+TGG | - | chr8.2:65221566-65221585 | None:intergenic | 45.0% | |
| GAAAGATGGACCCAAATGGA+AGG | + | chr8.2:65221267-65221286 | MS.gene57497:CDS | 45.0% | |
| GAATTGCGACATGTCGTTAC+CGG | - | chr8.2:65220879-65220898 | None:intergenic | 45.0% | |
| GAGAAAGAGAGTCCAAAGCA+AGG | + | chr8.2:65221551-65221570 | MS.gene57497:CDS | 45.0% | |
| GCAGCAAAAAGGAAGAGCAA+AGG | + | chr8.2:65221433-65221452 | MS.gene57497:CDS | 45.0% | |
| GGCCGTATTCAGAAGTTTGT+TGG | + | chr8.2:65221115-65221134 | MS.gene57497:CDS | 45.0% | |
| TGAGAATGATGTCTTGGCTG+AGG | + | chr8.2:65221180-65221199 | MS.gene57497:CDS | 45.0% | |
| ! | AGTCGGATTAGGCAGAAGTT+AGG | + | chr8.2:65221667-65221686 | MS.gene57497:intron | 45.0% |
| !! | ACGCTCTGAGAATGATGTCT+TGG | + | chr8.2:65221174-65221193 | MS.gene57497:CDS | 45.0% |
| !! | CAAAGGTTTCTAGTAGTGGG+GGG | + | chr8.2:65221320-65221339 | MS.gene57497:CDS | 45.0% |
| !!! | CTGCTTTTTGTCCCATTGGA+CGG | - | chr8.2:65221417-65221436 | None:intergenic | 45.0% |
| ATGTCTTGGCTGAGGCACAT+AGG | + | chr8.2:65221188-65221207 | MS.gene57497:CDS | 50.0% | |
| GGAGTCGACCAACAATCAGA+TGG | + | chr8.2:65221046-65221065 | MS.gene57497:CDS | 50.0% | |
| TGAACAGCCATCACCAGTAG+TGG | + | chr8.2:65221390-65221409 | MS.gene57497:CDS | 50.0% | |
| ACCAGTAGTGGTCCGTCCAA+TGG | + | chr8.2:65221402-65221421 | MS.gene57497:CDS | 55.0% | |
| CCAGTAGTGGTCCGTCCAAT+GGG | + | chr8.2:65221403-65221422 | MS.gene57497:CDS | 55.0% | |
| TCTAGCGGTGGTGTGGATTC+CGG | + | chr8.2:65220857-65220876 | MS.gene57497:CDS | 55.0% | |
| CCCATTGGACGGACCACTAC+TGG | - | chr8.2:65221406-65221425 | None:intergenic | 60.0% | |
| GGACGGACCACTACTGGTGA+TGG | - | chr8.2:65221400-65221419 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 65220851 | 65222193 | 65220851 | ID=MS.gene57497 |
| chr8.2 | mRNA | 65220851 | 65222193 | 65220851 | ID=MS.gene57497.t1;Parent=MS.gene57497 |
| chr8.2 | exon | 65220851 | 65221596 | 65220851 | ID=MS.gene57497.t1.exon1;Parent=MS.gene57497.t1 |
| chr8.2 | CDS | 65220851 | 65221596 | 65220851 | ID=cds.MS.gene57497.t1;Parent=MS.gene57497.t1 |
| chr8.2 | exon | 65222172 | 65222193 | 65222172 | ID=MS.gene57497.t1.exon2;Parent=MS.gene57497.t1 |
| chr8.2 | CDS | 65222172 | 65222193 | 65222172 | ID=cds.MS.gene57497.t1;Parent=MS.gene57497.t1 |
| Gene Sequence |
| Protein sequence |
