Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57515.t1 | XP_013444920.1 | 92.9 | 183 | 13 | 0 | 1 | 183 | 1 | 183 | 3.90E-92 | 347.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57515.t1 | A0A072TZI9 | 92.9 | 183 | 13 | 0 | 1 | 183 | 1 | 183 | 2.8e-92 | 347.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054222 | MS.gene57515 | 0.805255 | 1.46E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene57515 | 0.841567 | 4.39E-58 | -1.69E-46 |
| MS.gene054893 | MS.gene57515 | 0.845563 | 3.76E-59 | -1.69E-46 |
| MS.gene05952 | MS.gene57515 | 0.814854 | 1.25E-51 | -1.69E-46 |
| MS.gene060259 | MS.gene57515 | 0.808015 | 3.81E-50 | -1.69E-46 |
| MS.gene060412 | MS.gene57515 | 0.824398 | 8.25E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57515.t1 | MTR_8g432490 | 92.896 | 183 | 13 | 0 | 1 | 183 | 1 | 183 | 3.14e-125 | 350 |
| MS.gene57515.t1 | MTR_8g432460 | 88.525 | 183 | 21 | 0 | 1 | 183 | 1 | 183 | 2.90e-119 | 335 |
| MS.gene57515.t1 | MTR_8g432440 | 84.239 | 184 | 28 | 1 | 1 | 183 | 1 | 184 | 4.63e-112 | 317 |
| MS.gene57515.t1 | MTR_8g432420 | 84.239 | 184 | 28 | 1 | 1 | 183 | 1 | 184 | 4.63e-112 | 317 |
| MS.gene57515.t1 | MTR_3g064780 | 86.111 | 180 | 25 | 0 | 1 | 180 | 1 | 180 | 4.52e-111 | 317 |
| MS.gene57515.t1 | MTR_8g432470 | 82.609 | 184 | 31 | 1 | 1 | 183 | 1 | 184 | 2.89e-108 | 307 |
| MS.gene57515.t1 | MTR_8g432450 | 83.051 | 177 | 28 | 2 | 1 | 175 | 1 | 177 | 2.62e-102 | 292 |
| MS.gene57515.t1 | MTR_8g432480 | 51.136 | 176 | 64 | 5 | 5 | 174 | 7 | 166 | 7.90e-46 | 149 |
| MS.gene57515.t1 | MTR_7g076000 | 35.000 | 180 | 85 | 8 | 4 | 174 | 1 | 157 | 1.39e-22 | 89.4 |
| MS.gene57515.t1 | MTR_8g432400 | 35.870 | 184 | 101 | 6 | 4 | 179 | 1 | 175 | 2.12e-21 | 86.7 |
| MS.gene57515.t1 | MTR_8g432370 | 33.880 | 183 | 105 | 5 | 4 | 179 | 1 | 174 | 4.14e-20 | 83.2 |
| MS.gene57515.t1 | MTR_8g432350 | 33.702 | 181 | 104 | 5 | 4 | 177 | 1 | 172 | 2.01e-19 | 81.3 |
| MS.gene57515.t1 | MTR_8g432390 | 34.783 | 184 | 103 | 6 | 4 | 179 | 1 | 175 | 2.17e-19 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAATGGAGTTATTCATTT+TGG | 0.202103 | 8.3:-61877073 | MS.gene57515:CDS |
| AACTCTAATGGTGGTGTTAT+TGG | 0.227722 | 8.3:-61876938 | MS.gene57515:CDS |
| AACACCACCATTAGAGTTCT+TGG | 0.255915 | 8.3:+61876943 | None:intergenic |
| CCTGGGTAATTCCACCTAAA+AGG | 0.262534 | 8.3:+61876911 | None:intergenic |
| AGTTATGGTGATTGGTTAAC+TGG | 0.270173 | 8.3:-61876980 | MS.gene57515:CDS |
| AAAACTCGAGTTTCCTTTCA+TGG | 0.292723 | 8.3:-61877285 | MS.gene57515:CDS |
| GATTGGGAAGGGAGACAAAA+TGG | 0.327474 | 8.3:-61877088 | MS.gene57515:CDS |
| TTCAAAAGTGTTCGTCCTAT+TGG | 0.350867 | 8.3:-61877193 | MS.gene57515:CDS |
| TGAGTTATAAATTGAGGGAT+TGG | 0.355220 | 8.3:-61877105 | MS.gene57515:CDS |
| ATCGGTTACGGTGGCGGAAA+AGG | 0.359368 | 8.3:-61877023 | MS.gene57515:CDS |
| GCGATTCTTGCCACGCCAAT+AGG | 0.376994 | 8.3:+61877178 | None:intergenic |
| ATCATTATCTTTCACCGTCT+TGG | 0.400762 | 8.3:+61877318 | None:intergenic |
| ACAAATCTTTAAACAATGCC+TGG | 0.408723 | 8.3:+61876893 | None:intergenic |
| GCCGTTTCAGATATTCTAAA+CGG | 0.413963 | 8.3:-61877157 | MS.gene57515:CDS |
| ATGGAAAGAGTTATGGTGAT+TGG | 0.425777 | 8.3:-61876988 | MS.gene57515:CDS |
| TATTCTAAACGGGGCTGAAT+CGG | 0.426061 | 8.3:-61877146 | MS.gene57515:CDS |
| CCGTTTCAGATATTCTAAAC+GGG | 0.426321 | 8.3:-61877156 | MS.gene57515:CDS |
| GAAAACATCGGTTACGGTGG+CGG | 0.473639 | 8.3:-61877029 | MS.gene57515:CDS |
| GAGTTATAAATTGAGGGATT+GGG | 0.478191 | 8.3:-61877104 | MS.gene57515:CDS |
| AAAATTTCTCATTCCATGAA+AGG | 0.495676 | 8.3:+61877272 | None:intergenic |
| CTCACGCACATTAGAAATAA+CGG | 0.501464 | 8.3:-61877419 | MS.gene57515:CDS |
| GTGAAAGATAATGATGATTG+TGG | 0.518541 | 8.3:-61877310 | MS.gene57515:CDS |
| TTAGAAATAACGGTTATATC+CGG | 0.522816 | 8.3:-61877409 | MS.gene57515:CDS |
| ATGGCGGATGGAAAGAGTTA+TGG | 0.523652 | 8.3:-61876995 | MS.gene57515:CDS |
| GTTGGTACCAAGAACTCTAA+TGG | 0.534197 | 8.3:-61876950 | MS.gene57515:CDS |
| GATGCATACGTTGTTGTTCG+AGG | 0.534726 | 8.3:-61877364 | MS.gene57515:CDS |
| CCCGTTTAGAATATCTGAAA+CGG | 0.537134 | 8.3:+61877156 | None:intergenic |
| ATAAATTGAGGGATTGGGAA+GGG | 0.539517 | 8.3:-61877099 | MS.gene57515:CDS |
| CAAATCTTTAAACAATGCCT+GGG | 0.558166 | 8.3:+61876894 | None:intergenic |
| TGAAAGATAATGATGATTGT+GGG | 0.561848 | 8.3:-61877309 | MS.gene57515:CDS |
| TGAACATGCCATTGCATGCA+AGG | 0.566598 | 8.3:-61877246 | MS.gene57515:CDS |
| GCCAGAGAAAACATCGGTTA+CGG | 0.572228 | 8.3:-61877035 | MS.gene57515:CDS |
| TATAAATTGAGGGATTGGGA+AGG | 0.575657 | 8.3:-61877100 | MS.gene57515:CDS |
| TGGTTAACTGGAATTGATGT+TGG | 0.596345 | 8.3:-61876968 | MS.gene57515:CDS |
| GTGATTGACCTTGCATGCAA+TGG | 0.600206 | 8.3:+61877238 | None:intergenic |
| GAAAAGGAAACAATGGCGGA+TGG | 0.603995 | 8.3:-61877007 | MS.gene57515:CDS |
| TGAAAATATTCATGTAACTG+AGG | 0.625588 | 8.3:-61877386 | MS.gene57515:CDS |
| CGTTTCAGATATTCTAAACG+GGG | 0.637516 | 8.3:-61877155 | MS.gene57515:CDS |
| AAGTGTTCGTCCTATTGGCG+TGG | 0.646267 | 8.3:-61877188 | MS.gene57515:CDS |
| GGTGGCGGAAAAGGAAACAA+TGG | 0.668224 | 8.3:-61877014 | MS.gene57515:CDS |
| AGAGAAAACATCGGTTACGG+TGG | 0.680613 | 8.3:-61877032 | MS.gene57515:CDS |
| GGCGGAAAAGGAAACAATGG+CGG | 0.701876 | 8.3:-61877011 | MS.gene57515:CDS |
| TACTTGTTACACGACCAAGA+CGG | 0.705041 | 8.3:-61877332 | MS.gene57515:CDS |
| GGTTGTGCCAGAGAAAACAT+CGG | 0.723045 | 8.3:-61877041 | MS.gene57515:CDS |
| GGTACCAAGAACTCTAATGG+TGG | 0.759836 | 8.3:-61876947 | MS.gene57515:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTGAGTTATAAATTGA+GGG | - | chr8.3:61877221-61877240 | MS.gene57515:CDS | 15.0% |
| !!! | GAATTTTGAGTTATAAATTG+AGG | - | chr8.3:61877220-61877239 | MS.gene57515:CDS | 20.0% |
| ! | AAAATTTCTCATTCCATGAA+AGG | + | chr8.3:61877062-61877081 | None:intergenic | 25.0% |
| ! | TGAAAATATTCATGTAACTG+AGG | - | chr8.3:61876945-61876964 | MS.gene57515:CDS | 25.0% |
| ! | TGAAAGATAATGATGATTGT+GGG | - | chr8.3:61877022-61877041 | MS.gene57515:CDS | 25.0% |
| ! | TTAGAAATAACGGTTATATC+CGG | - | chr8.3:61876922-61876941 | MS.gene57515:CDS | 25.0% |
| !! | AGTTACATGAATATTTTCAC+CGG | + | chr8.3:61876944-61876963 | None:intergenic | 25.0% |
| !! | CAAAATGGAGTTATTCATTT+TGG | - | chr8.3:61877258-61877277 | MS.gene57515:CDS | 25.0% |
| GAGTTATAAATTGAGGGATT+GGG | - | chr8.3:61877227-61877246 | MS.gene57515:CDS | 30.0% | |
| GTGAAAGATAATGATGATTG+TGG | - | chr8.3:61877021-61877040 | MS.gene57515:CDS | 30.0% | |
| TGAGTTATAAATTGAGGGAT+TGG | - | chr8.3:61877226-61877245 | MS.gene57515:CDS | 30.0% | |
| ! | TTATTGGTATTCCTTTTAGG+TGG | - | chr8.3:61877409-61877428 | MS.gene57515:CDS | 30.0% |
| !! | AGTTATTCATTTTGGTGTGA+GGG | - | chr8.3:61877266-61877285 | MS.gene57515:CDS | 30.0% |
| !! | GTGTTATTGGTATTCCTTTT+AGG | - | chr8.3:61877406-61877425 | MS.gene57515:CDS | 30.0% |
| AAAACTCGAGTTTCCTTTCA+TGG | - | chr8.3:61877046-61877065 | MS.gene57515:CDS | 35.0% | |
| ATAAATTGAGGGATTGGGAA+GGG | - | chr8.3:61877232-61877251 | MS.gene57515:CDS | 35.0% | |
| ATCATTATCTTTCACCGTCT+TGG | + | chr8.3:61877016-61877035 | None:intergenic | 35.0% | |
| ATGGAAAGAGTTATGGTGAT+TGG | - | chr8.3:61877343-61877362 | MS.gene57515:CDS | 35.0% | |
| CCGTTTCAGATATTCTAAAC+GGG | - | chr8.3:61877175-61877194 | MS.gene57515:CDS | 35.0% | |
| CGTTTCAGATATTCTAAACG+GGG | - | chr8.3:61877176-61877195 | MS.gene57515:CDS | 35.0% | |
| CTCACGCACATTAGAAATAA+CGG | - | chr8.3:61876912-61876931 | MS.gene57515:CDS | 35.0% | |
| GCCGTTTCAGATATTCTAAA+CGG | - | chr8.3:61877174-61877193 | MS.gene57515:CDS | 35.0% | |
| TATAAATTGAGGGATTGGGA+AGG | - | chr8.3:61877231-61877250 | MS.gene57515:CDS | 35.0% | |
| TGGTTAACTGGAATTGATGT+TGG | - | chr8.3:61877363-61877382 | MS.gene57515:CDS | 35.0% | |
| ! | AGTTATGGTGATTGGTTAAC+TGG | - | chr8.3:61877351-61877370 | MS.gene57515:CDS | 35.0% |
| ! | CCCGTTTAGAATATCTGAAA+CGG | + | chr8.3:61877178-61877197 | None:intergenic | 35.0% |
| !! | AACTCTAATGGTGGTGTTAT+TGG | - | chr8.3:61877393-61877412 | MS.gene57515:CDS | 35.0% |
| !! | GAGTTATTCATTTTGGTGTG+AGG | - | chr8.3:61877265-61877284 | MS.gene57515:CDS | 35.0% |
| !! | TTCAAAAGTGTTCGTCCTAT+TGG | - | chr8.3:61877138-61877157 | MS.gene57515:CDS | 35.0% |
| AACACCACCATTAGAGTTCT+TGG | + | chr8.3:61877391-61877410 | None:intergenic | 40.0% | |
| GTTGGTACCAAGAACTCTAA+TGG | - | chr8.3:61877381-61877400 | MS.gene57515:CDS | 40.0% | |
| TATTCTAAACGGGGCTGAAT+CGG | - | chr8.3:61877185-61877204 | MS.gene57515:CDS | 40.0% | |
| ! | TACTTGTTACACGACCAAGA+CGG | - | chr8.3:61876999-61877018 | MS.gene57515:CDS | 40.0% |
| !! | TATTCATTTTGGTGTGAGGG+TGG | - | chr8.3:61877269-61877288 | MS.gene57515:CDS | 40.0% |
| !! | TGCAAGGTCAATCACTTTTG+AGG | - | chr8.3:61877101-61877120 | MS.gene57515:CDS | 40.0% |
| CCTGGGTAATTCCACCTAAA+AGG | + | chr8.3:61877423-61877442 | None:intergenic | 45.0% | |
| GATTGGGAAGGGAGACAAAA+TGG | - | chr8.3:61877243-61877262 | MS.gene57515:CDS | 45.0% | |
| GGTACCAAGAACTCTAATGG+TGG | - | chr8.3:61877384-61877403 | MS.gene57515:CDS | 45.0% | |
| GGTTGTGCCAGAGAAAACAT+CGG | - | chr8.3:61877290-61877309 | MS.gene57515:CDS | 45.0% | |
| GTGATTGACCTTGCATGCAA+TGG | + | chr8.3:61877096-61877115 | None:intergenic | 45.0% | |
| TGAACATGCCATTGCATGCA+AGG | - | chr8.3:61877085-61877104 | MS.gene57515:CDS | 45.0% | |
| ! | ACCGTAACCGATGTTTTCTC+TGG | + | chr8.3:61877300-61877319 | None:intergenic | 45.0% |
| ! | AGAGAAAACATCGGTTACGG+TGG | - | chr8.3:61877299-61877318 | MS.gene57515:CDS | 45.0% |
| ! | GAAAAGGAAACAATGGCGGA+TGG | - | chr8.3:61877324-61877343 | MS.gene57515:CDS | 45.0% |
| ! | GATGCATACGTTGTTGTTCG+AGG | - | chr8.3:61876967-61876986 | MS.gene57515:CDS | 45.0% |
| ! | GCCAGAGAAAACATCGGTTA+CGG | - | chr8.3:61877296-61877315 | MS.gene57515:CDS | 45.0% |
| !! | ATGGCGGATGGAAAGAGTTA+TGG | - | chr8.3:61877336-61877355 | MS.gene57515:CDS | 45.0% |
| !! | CCTTTTAGGTGGAATTACCC+AGG | - | chr8.3:61877420-61877439 | MS.gene57515:CDS | 45.0% |
| AAGTGTTCGTCCTATTGGCG+TGG | - | chr8.3:61877143-61877162 | MS.gene57515:CDS | 50.0% | |
| GGCGGAAAAGGAAACAATGG+CGG | - | chr8.3:61877320-61877339 | MS.gene57515:CDS | 50.0% | |
| ! | GAAAACATCGGTTACGGTGG+CGG | - | chr8.3:61877302-61877321 | MS.gene57515:CDS | 50.0% |
| !! | GGTGGCGGAAAAGGAAACAA+TGG | - | chr8.3:61877317-61877336 | MS.gene57515:CDS | 50.0% |
| GCGATTCTTGCCACGCCAAT+AGG | + | chr8.3:61877156-61877175 | None:intergenic | 55.0% | |
| !! | ATCGGTTACGGTGGCGGAAA+AGG | - | chr8.3:61877308-61877327 | MS.gene57515:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 61876901 | 61877452 | 61876901 | ID=MS.gene57515 |
| chr8.3 | mRNA | 61876901 | 61877452 | 61876901 | ID=MS.gene57515.t1;Parent=MS.gene57515 |
| chr8.3 | exon | 61876901 | 61877452 | 61876901 | ID=MS.gene57515.t1.exon1;Parent=MS.gene57515.t1 |
| chr8.3 | CDS | 61876901 | 61877452 | 61876901 | ID=cds.MS.gene57515.t1;Parent=MS.gene57515.t1 |
| Gene Sequence |
| Protein sequence |
