Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57517.t1 | XP_013444917.1 | 88.5 | 183 | 19 | 1 | 1 | 181 | 1 | 183 | 2.00E-88 | 335.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57517.t1 | A0A072TNG9 | 88.5 | 183 | 19 | 1 | 1 | 181 | 1 | 183 | 1.4e-88 | 335.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051226 | MS.gene57517 | 0.86021 | 2.42E-63 | -1.69E-46 |
| MS.gene054892 | MS.gene57517 | 0.813102 | 3.03E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene57517 | 0.818423 | 1.98E-52 | -1.69E-46 |
| MS.gene057093 | MS.gene57517 | 0.833365 | 5.56E-56 | -1.69E-46 |
| MS.gene057167 | MS.gene57517 | 0.832884 | 7.32E-56 | -1.69E-46 |
| MS.gene057622 | MS.gene57517 | 0.840023 | 1.12E-57 | -1.69E-46 |
| MS.gene06023 | MS.gene57517 | 0.816633 | 5.00E-52 | -1.69E-46 |
| MS.gene060964 | MS.gene57517 | 0.805545 | 1.27E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57517.t1 | MTR_8g432460 | 88.525 | 183 | 19 | 1 | 1 | 181 | 1 | 183 | 9.63e-120 | 336 |
| MS.gene57517.t1 | MTR_8g432470 | 88.587 | 184 | 18 | 2 | 1 | 181 | 1 | 184 | 2.16e-116 | 328 |
| MS.gene57517.t1 | MTR_8g432490 | 86.339 | 183 | 23 | 1 | 1 | 181 | 1 | 183 | 1.12e-115 | 326 |
| MS.gene57517.t1 | MTR_8g432440 | 86.957 | 184 | 21 | 2 | 1 | 181 | 1 | 184 | 5.55e-115 | 324 |
| MS.gene57517.t1 | MTR_8g432420 | 86.957 | 184 | 21 | 2 | 1 | 181 | 1 | 184 | 5.55e-115 | 324 |
| MS.gene57517.t1 | MTR_8g432450 | 89.595 | 173 | 16 | 2 | 1 | 171 | 1 | 173 | 7.89e-109 | 308 |
| MS.gene57517.t1 | MTR_3g064780 | 82.222 | 180 | 30 | 1 | 1 | 178 | 1 | 180 | 1.82e-108 | 310 |
| MS.gene57517.t1 | MTR_8g432480 | 50.847 | 177 | 63 | 7 | 5 | 173 | 7 | 167 | 9.98e-46 | 149 |
| MS.gene57517.t1 | MTR_7g076000 | 37.017 | 181 | 80 | 9 | 5 | 174 | 2 | 159 | 2.29e-24 | 94.0 |
| MS.gene57517.t1 | MTR_8g432400 | 40.152 | 132 | 69 | 3 | 5 | 130 | 2 | 129 | 4.45e-21 | 85.5 |
| MS.gene57517.t1 | MTR_8g432370 | 38.168 | 131 | 72 | 2 | 5 | 130 | 2 | 128 | 1.99e-20 | 84.0 |
| MS.gene57517.t1 | MTR_8g432350 | 38.636 | 132 | 71 | 3 | 5 | 130 | 2 | 129 | 7.58e-20 | 82.4 |
| MS.gene57517.t1 | MTR_8g432390 | 39.394 | 132 | 70 | 3 | 5 | 130 | 2 | 129 | 9.17e-20 | 82.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 50 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTATGGTGGTCGTTTAAC+TGG | 0.233341 | 8.3:-61882914 | MS.gene57517:CDS |
| ACAAATCTTTAAATAATGTT+AGG | 0.260927 | 8.3:+61882833 | None:intergenic |
| TTCAAAAGTGTTCGTCCTAT+TGG | 0.279263 | 8.3:-61883127 | MS.gene57517:CDS |
| AAAATTCGAGTTTCCTTTCA+TGG | 0.287575 | 8.3:-61883219 | MS.gene57517:CDS |
| TCAAAAGTGTTCGTCCTATT+GGG | 0.298427 | 8.3:-61883126 | MS.gene57517:CDS |
| GTGGCGGATGGAAAGAATTA+TGG | 0.368827 | 8.3:-61882929 | MS.gene57517:CDS |
| GATTGGGAAGGGAAGCAAAA+TGG | 0.374296 | 8.3:-61883022 | MS.gene57517:CDS |
| TGAGCTATAAATTGAGGGAT+TGG | 0.377003 | 8.3:-61883039 | MS.gene57517:CDS |
| GCAATTCTTGCCACCCCAAT+AGG | 0.383363 | 8.3:+61883112 | None:intergenic |
| GTTAGGGTAATTCCACCAAA+TGG | 0.387713 | 8.3:+61882850 | None:intergenic |
| GGTGGCAAGAATTGCCGTTT+CGG | 0.415973 | 8.3:-61883104 | MS.gene57517:CDS |
| ATGGTGTTGGTATTCCCATT+TGG | 0.447342 | 8.3:-61882865 | MS.gene57517:CDS |
| CAAATCTTTAAATAATGTTA+GGG | 0.457538 | 8.3:+61882834 | None:intergenic |
| GAGCTATAAATTGAGGGATT+GGG | 0.461893 | 8.3:-61883038 | MS.gene57517:CDS |
| TAGTGTGTGGGTGGTTGTGC+CGG | 0.479175 | 8.3:-61882987 | MS.gene57517:CDS |
| TTAGAAATCACGGTTATATC+CGG | 0.498692 | 8.3:-61883343 | MS.gene57517:CDS |
| TGGTGGTCGTTTAACTGGAA+TGG | 0.500801 | 8.3:-61882909 | MS.gene57517:CDS |
| CACCGTTTGCTTCTCCGCCA+CGG | 0.511604 | 8.3:+61882949 | None:intergenic |
| GGCGGAGAAGCAAACGGTGG+CGG | 0.515203 | 8.3:-61882945 | MS.gene57517:CDS |
| TTAGGGTAATTCCACCAAAT+GGG | 0.516321 | 8.3:+61882851 | None:intergenic |
| TTAATTCTCTACTATGGGTG+TGG | 0.519159 | 8.3:-61883377 | None:intergenic |
| CCCGTTTAAAATATCCGAAA+CGG | 0.521654 | 8.3:+61883090 | None:intergenic |
| TGCAAGGTCAATCACGTTTG+AGG | 0.523501 | 8.3:-61883164 | MS.gene57517:CDS |
| GTGAAAGATAATGATGATTG+TGG | 0.524560 | 8.3:-61883244 | MS.gene57517:CDS |
| GAGAAGCAAACGGTGGCGGA+TGG | 0.538736 | 8.3:-61882941 | MS.gene57517:CDS |
| GTGTTGGTATTCCCATTTGG+TGG | 0.543771 | 8.3:-61882862 | MS.gene57517:CDS |
| AAAACTTCTCATTCCATGAA+AGG | 0.547930 | 8.3:+61883206 | None:intergenic |
| CGTTTAACTGGAATGGATGC+TGG | 0.557942 | 8.3:-61882902 | MS.gene57517:CDS |
| TGAAAGATAATGATGATTGT+GGG | 0.561848 | 8.3:-61883243 | MS.gene57517:CDS |
| CAAAAGTGTTCGTCCTATTG+GGG | 0.562181 | 8.3:-61883125 | MS.gene57517:CDS |
| AATGGGAATACCAACACCAT+TGG | 0.564682 | 8.3:+61882868 | None:intergenic |
| TGAACATGCCATTGCATGCA+AGG | 0.566598 | 8.3:-61883180 | MS.gene57517:CDS |
| GATGCATACGTTGTTGTTCG+AGG | 0.567581 | 8.3:-61883298 | MS.gene57517:CDS |
| GGTTGTGCCGGAGCAAAGAT+CGG | 0.574606 | 8.3:-61882975 | MS.gene57517:CDS |
| TACCGTGGCGGAGAAGCAAA+CGG | 0.575684 | 8.3:-61882951 | MS.gene57517:CDS |
| ACGGTAACCGATCTTTGCTC+CGG | 0.585792 | 8.3:+61882968 | None:intergenic |
| ATAAATTGAGGGATTGGGAA+GGG | 0.595139 | 8.3:-61883033 | MS.gene57517:CDS |
| TATAAATTGAGGGATTGGGA+AGG | 0.599766 | 8.3:-61883034 | MS.gene57517:CDS |
| CTCACGTACATTAGAAATCA+CGG | 0.602536 | 8.3:-61883353 | MS.gene57517:CDS |
| CGGCGAAAATGTTCATGTAA+CGG | 0.607120 | 8.3:-61883323 | MS.gene57517:CDS |
| GTGATTGACCTTGCATGCAA+TGG | 0.618595 | 8.3:+61883172 | None:intergenic |
| GTCAAGAACTCCAATGGTGT+TGG | 0.622339 | 8.3:-61882878 | MS.gene57517:CDS |
| AAGTGTTCGTCCTATTGGGG+TGG | 0.646267 | 8.3:-61883122 | MS.gene57517:CDS |
| CGTGATTTCTAATGTACGTG+AGG | 0.647152 | 8.3:+61883354 | None:intergenic |
| GCAAAGATCGGTTACCGTGG+CGG | 0.653482 | 8.3:-61882963 | MS.gene57517:CDS |
| GCGGATGGAAAGAATTATGG+TGG | 0.668878 | 8.3:-61882926 | MS.gene57517:CDS |
| CGAAAATGTTCATGTAACGG+AGG | 0.688601 | 8.3:-61883320 | MS.gene57517:CDS |
| GGAGCAAAGATCGGTTACCG+TGG | 0.694711 | 8.3:-61882966 | MS.gene57517:CDS |
| GCTGGTGTCAAGAACTCCAA+TGG | 0.697227 | 8.3:-61882884 | MS.gene57517:CDS |
| CGTGGCGGAGAAGCAAACGG+TGG | 0.751871 | 8.3:-61882948 | MS.gene57517:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTTGAGCTATAAATTG+AGG | - | chr8.3:61883160-61883179 | MS.gene57517:CDS | 20.0% |
| !!! | AATTTTGAGCTATAAATTGA+GGG | - | chr8.3:61883161-61883180 | MS.gene57517:CDS | 20.0% |
| ! | TAATTGTTACACCACAAAAA+CGG | - | chr8.3:61882939-61882958 | MS.gene57517:CDS | 25.0% |
| ! | TGAAAGATAATGATGATTGT+GGG | - | chr8.3:61882962-61882981 | MS.gene57517:CDS | 25.0% |
| !!! | AGTTATTCATTTTAGTGTGT+GGG | - | chr8.3:61883206-61883225 | MS.gene57517:CDS | 25.0% |
| AAAACTTCTCATTCCATGAA+AGG | + | chr8.3:61883002-61883021 | None:intergenic | 30.0% | |
| GTGAAAGATAATGATGATTG+TGG | - | chr8.3:61882961-61882980 | MS.gene57517:CDS | 30.0% | |
| TTAGAAATCACGGTTATATC+CGG | - | chr8.3:61882862-61882881 | MS.gene57517:CDS | 30.0% | |
| ! | AAAATTCGAGTTTCCTTTCA+TGG | - | chr8.3:61882986-61883005 | MS.gene57517:CDS | 30.0% |
| !! | ATTATCTTTCACCGTTTTTG+TGG | + | chr8.3:61882953-61882972 | None:intergenic | 30.0% |
| !! | GAGTTATTCATTTTAGTGTG+TGG | - | chr8.3:61883205-61883224 | MS.gene57517:CDS | 30.0% |
| !! | TATTTTAAACGGGGATGAAT+CGG | - | chr8.3:61883125-61883144 | MS.gene57517:CDS | 30.0% |
| AATTATGGTGGTCGTTTAAC+TGG | - | chr8.3:61883291-61883310 | MS.gene57517:CDS | 35.0% | |
| ATAAATTGAGGGATTGGGAA+GGG | - | chr8.3:61883172-61883191 | MS.gene57517:CDS | 35.0% | |
| CTCACGTACATTAGAAATCA+CGG | - | chr8.3:61882852-61882871 | MS.gene57517:CDS | 35.0% | |
| GAGCTATAAATTGAGGGATT+GGG | - | chr8.3:61883167-61883186 | MS.gene57517:CDS | 35.0% | |
| TATAAATTGAGGGATTGGGA+AGG | - | chr8.3:61883171-61883190 | MS.gene57517:CDS | 35.0% | |
| TGAGCTATAAATTGAGGGAT+TGG | - | chr8.3:61883166-61883185 | MS.gene57517:CDS | 35.0% | |
| TTAGGGTAATTCCACCAAAT+GGG | + | chr8.3:61883357-61883376 | None:intergenic | 35.0% | |
| ! | CCCGTTTAAAATATCCGAAA+CGG | + | chr8.3:61883118-61883137 | None:intergenic | 35.0% |
| !! | CCGTTTCGGATATTTTAAAC+GGG | - | chr8.3:61883115-61883134 | MS.gene57517:CDS | 35.0% |
| !! | CGTTTCGGATATTTTAAACG+GGG | - | chr8.3:61883116-61883135 | MS.gene57517:CDS | 35.0% |
| !! | GCCGTTTCGGATATTTTAAA+CGG | - | chr8.3:61883114-61883133 | MS.gene57517:CDS | 35.0% |
| !! | TATTCATTTTAGTGTGTGGG+TGG | - | chr8.3:61883209-61883228 | MS.gene57517:CDS | 35.0% |
| !! | TCAAAAGTGTTCGTCCTATT+GGG | - | chr8.3:61883079-61883098 | MS.gene57517:CDS | 35.0% |
| !! | TTCAAAAGTGTTCGTCCTAT+TGG | - | chr8.3:61883078-61883097 | MS.gene57517:CDS | 35.0% |
| AATGGGAATACCAACACCAT+TGG | + | chr8.3:61883340-61883359 | None:intergenic | 40.0% | |
| CGAAAATGTTCATGTAACGG+AGG | - | chr8.3:61882885-61882904 | MS.gene57517:CDS | 40.0% | |
| CGGCGAAAATGTTCATGTAA+CGG | - | chr8.3:61882882-61882901 | MS.gene57517:CDS | 40.0% | |
| CGTGATTTCTAATGTACGTG+AGG | + | chr8.3:61882854-61882873 | None:intergenic | 40.0% | |
| GTTAGGGTAATTCCACCAAA+TGG | + | chr8.3:61883358-61883377 | None:intergenic | 40.0% | |
| ! | CGTTACATGAACATTTTCGC+CGG | + | chr8.3:61882884-61882903 | None:intergenic | 40.0% |
| !! | ATGGTGTTGGTATTCCCATT+TGG | - | chr8.3:61883340-61883359 | MS.gene57517:CDS | 40.0% |
| !! | CAAAAGTGTTCGTCCTATTG+GGG | - | chr8.3:61883080-61883099 | MS.gene57517:CDS | 40.0% |
| CGTTTAACTGGAATGGATGC+TGG | - | chr8.3:61883303-61883322 | MS.gene57517:CDS | 45.0% | |
| GATTGGGAAGGGAAGCAAAA+TGG | - | chr8.3:61883183-61883202 | MS.gene57517:CDS | 45.0% | |
| GCGGATGGAAAGAATTATGG+TGG | - | chr8.3:61883279-61883298 | MS.gene57517:CDS | 45.0% | |
| GTCAAGAACTCCAATGGTGT+TGG | - | chr8.3:61883327-61883346 | MS.gene57517:CDS | 45.0% | |
| GTGATTGACCTTGCATGCAA+TGG | + | chr8.3:61883036-61883055 | None:intergenic | 45.0% | |
| TGAACATGCCATTGCATGCA+AGG | - | chr8.3:61883025-61883044 | MS.gene57517:CDS | 45.0% | |
| TGCAAGGTCAATCACGTTTG+AGG | - | chr8.3:61883041-61883060 | MS.gene57517:CDS | 45.0% | |
| TGGTGGTCGTTTAACTGGAA+TGG | - | chr8.3:61883296-61883315 | MS.gene57517:CDS | 45.0% | |
| ! | GATGCATACGTTGTTGTTCG+AGG | - | chr8.3:61882907-61882926 | MS.gene57517:CDS | 45.0% |
| ! | GTGTTGGTATTCCCATTTGG+TGG | - | chr8.3:61883343-61883362 | MS.gene57517:CDS | 45.0% |
| !! | GTGGCGGATGGAAAGAATTA+TGG | - | chr8.3:61883276-61883295 | MS.gene57517:CDS | 45.0% |
| AAGTGTTCGTCCTATTGGGG+TGG | - | chr8.3:61883083-61883102 | MS.gene57517:CDS | 50.0% | |
| ACGGTAACCGATCTTTGCTC+CGG | + | chr8.3:61883240-61883259 | None:intergenic | 50.0% | |
| GCAATTCTTGCCACCCCAAT+AGG | + | chr8.3:61883096-61883115 | None:intergenic | 50.0% | |
| ! | GCTGGTGTCAAGAACTCCAA+TGG | - | chr8.3:61883321-61883340 | MS.gene57517:CDS | 50.0% |
| ! | GGTGGCAAGAATTGCCGTTT+CGG | - | chr8.3:61883101-61883120 | MS.gene57517:CDS | 50.0% |
| GCAAAGATCGGTTACCGTGG+CGG | - | chr8.3:61883242-61883261 | MS.gene57517:CDS | 55.0% | |
| GGAGCAAAGATCGGTTACCG+TGG | - | chr8.3:61883239-61883258 | MS.gene57517:CDS | 55.0% | |
| GGTTGTGCCGGAGCAAAGAT+CGG | - | chr8.3:61883230-61883249 | MS.gene57517:CDS | 55.0% | |
| TAGTGTGTGGGTGGTTGTGC+CGG | - | chr8.3:61883218-61883237 | MS.gene57517:CDS | 55.0% | |
| ! | TACCGTGGCGGAGAAGCAAA+CGG | - | chr8.3:61883254-61883273 | MS.gene57517:CDS | 55.0% |
| CACCGTTTGCTTCTCCGCCA+CGG | + | chr8.3:61883259-61883278 | None:intergenic | 60.0% | |
| !! | GAGAAGCAAACGGTGGCGGA+TGG | - | chr8.3:61883264-61883283 | MS.gene57517:CDS | 60.0% |
| GGCGGAGAAGCAAACGGTGG+CGG | - | chr8.3:61883260-61883279 | MS.gene57517:CDS | 65.0% | |
| ! | CGTGGCGGAGAAGCAAACGG+TGG | - | chr8.3:61883257-61883276 | MS.gene57517:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 61882841 | 61883386 | 61882841 | ID=MS.gene57517 |
| chr8.3 | mRNA | 61882841 | 61883386 | 61882841 | ID=MS.gene57517.t1;Parent=MS.gene57517 |
| chr8.3 | exon | 61882841 | 61883386 | 61882841 | ID=MS.gene57517.t1.exon1;Parent=MS.gene57517.t1 |
| chr8.3 | CDS | 61882841 | 61883386 | 61882841 | ID=cds.MS.gene57517.t1;Parent=MS.gene57517.t1 |
| Gene Sequence |
| Protein sequence |
