Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57521.t1 | XP_013444911.1 | 92.2 | 179 | 13 | 1 | 1 | 178 | 1 | 179 | 3.70E-87 | 330.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57521.t1 | Q58FX0 | 36.9 | 203 | 94 | 7 | 3 | 176 | 8 | 205 | 4.2e-23 | 109.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57521.t1 | A0A072TP32 | 92.2 | 179 | 13 | 1 | 1 | 178 | 1 | 179 | 2.7e-87 | 330.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene57521 | 0.821257 | 4.45E-53 | -1.69E-46 |
| MS.gene054892 | MS.gene57521 | 0.808406 | 3.15E-50 | -1.69E-46 |
| MS.gene054893 | MS.gene57521 | 0.802673 | 5.02E-49 | -1.69E-46 |
| MS.gene061047 | MS.gene57521 | 0.810387 | 1.18E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57521.t1 | MTR_8g432390 | 88.268 | 179 | 20 | 1 | 1 | 178 | 1 | 179 | 1.25e-111 | 315 |
| MS.gene57521.t1 | MTR_8g432350 | 86.592 | 179 | 23 | 1 | 1 | 178 | 1 | 179 | 4.38e-110 | 311 |
| MS.gene57521.t1 | MTR_8g432400 | 87.709 | 179 | 21 | 1 | 1 | 178 | 1 | 179 | 7.74e-109 | 308 |
| MS.gene57521.t1 | MTR_8g432370 | 86.592 | 179 | 22 | 2 | 1 | 178 | 1 | 178 | 3.25e-108 | 306 |
| MS.gene57521.t1 | MTR_7g076000 | 52.486 | 181 | 67 | 5 | 1 | 177 | 1 | 166 | 3.11e-54 | 169 |
| MS.gene57521.t1 | MTR_8g432440 | 36.957 | 184 | 97 | 6 | 2 | 174 | 5 | 180 | 5.29e-27 | 100 |
| MS.gene57521.t1 | MTR_8g432420 | 36.957 | 184 | 97 | 6 | 2 | 174 | 5 | 180 | 5.29e-27 | 100 |
| MS.gene57521.t1 | MTR_8g432470 | 36.957 | 184 | 97 | 6 | 2 | 174 | 5 | 180 | 7.25e-26 | 98.2 |
| MS.gene57521.t1 | MTR_8g432450 | 35.393 | 178 | 98 | 4 | 2 | 168 | 5 | 176 | 1.90e-24 | 94.4 |
| MS.gene57521.t1 | MTR_8g432460 | 34.973 | 183 | 101 | 6 | 2 | 174 | 5 | 179 | 4.53e-22 | 88.2 |
| MS.gene57521.t1 | MTR_8g432490 | 34.973 | 183 | 101 | 6 | 2 | 174 | 5 | 179 | 5.89e-21 | 85.1 |
| MS.gene57521.t1 | MTR_3g064780 | 34.973 | 183 | 101 | 7 | 2 | 174 | 5 | 179 | 1.51e-20 | 85.5 |
| MS.gene57521.t1 | MTR_8g432480 | 32.530 | 166 | 98 | 4 | 2 | 156 | 7 | 169 | 3.58e-19 | 80.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57521.t1 | AT2G45760 | 36.585 | 205 | 96 | 8 | 1 | 176 | 6 | 205 | 2.48e-32 | 115 |
| MS.gene57521.t1 | AT3G61190 | 38.462 | 182 | 103 | 5 | 1 | 178 | 16 | 192 | 1.39e-30 | 110 |
| MS.gene57521.t1 | AT3G61190 | 38.462 | 182 | 103 | 5 | 1 | 178 | 16 | 192 | 1.39e-30 | 110 |
Find 47 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACAACAAAGGCATAAA+AGG | 0.174415 | 8.3:-61917793 | MS.gene57521:CDS |
| CACCCTTTCAAATGTGTCTT+TGG | 0.267837 | 8.3:+61917320 | None:intergenic |
| TTATGCCTTTGTTGTTCTCT+TGG | 0.294333 | 8.3:+61917797 | None:intergenic |
| ATAATAGAGTTAGGAGAAAT+GGG | 0.321912 | 8.3:-61917501 | MS.gene57521:CDS |
| GATAATAGAGTTAGGAGAAA+TGG | 0.337385 | 8.3:-61917502 | MS.gene57521:CDS |
| CAACAGGGATTCCTGCTGTT+TGG | 0.341709 | 8.3:-61917351 | MS.gene57521:CDS |
| GGTGTTCCGGTGGCCGGTAA+TGG | 0.375336 | 8.3:-61917391 | MS.gene57521:CDS |
| TCCTGCAGCAGAGTTGCAAC+AGG | 0.395408 | 8.3:-61917367 | MS.gene57521:CDS |
| GTCATCGATGAGTGGAGTTC+CGG | 0.397704 | 8.3:-61917419 | MS.gene57521:CDS |
| AATTAGAACAAGAACTTTGT+TGG | 0.403293 | 8.3:+61917446 | None:intergenic |
| ATTAGAACAAGAACTTTGTT+GGG | 0.411878 | 8.3:+61917447 | None:intergenic |
| ATGAGTGGAGTTCCGGTAAC+TGG | 0.413867 | 8.3:-61917412 | MS.gene57521:CDS |
| TACATAACCTCCAAGAAAAT+CGG | 0.422382 | 8.3:+61917564 | None:intergenic |
| CCCTGTTGCAACTCTGCTGC+AGG | 0.438745 | 8.3:+61917366 | None:intergenic |
| TCTGCTGCAGGAGCCATTAC+CGG | 0.444058 | 8.3:+61917378 | None:intergenic |
| AGTTCCGGTAACTGGTGTTC+CGG | 0.450510 | 8.3:-61917404 | MS.gene57521:CDS |
| TGTAGGGTAACTTCCGCCTT+CGG | 0.459071 | 8.3:+61917705 | None:intergenic |
| GGTTGTGGGATAATAGAGTT+AGG | 0.466052 | 8.3:-61917510 | MS.gene57521:CDS |
| TGGTAGTTTAACCAAACAGC+AGG | 0.487555 | 8.3:+61917340 | None:intergenic |
| GCCTTCGGAATCAAGTTTCG+TGG | 0.489360 | 8.3:+61917720 | None:intergenic |
| GTTATGAACCTTGCATGTAA+TGG | 0.492338 | 8.3:+61917655 | None:intergenic |
| AGAACAACAAAGGCATAAAA+GGG | 0.500399 | 8.3:-61917792 | MS.gene57521:CDS |
| GCCACCGGAACACCAGTTAC+CGG | 0.504701 | 8.3:+61917400 | None:intergenic |
| CCAACTTCTCGTTCCATGTA+GGG | 0.504708 | 8.3:+61917689 | None:intergenic |
| TAGTAGTAATAGTGTTGGTA+TGG | 0.504773 | 8.3:-61917602 | MS.gene57521:CDS |
| GTAACTGGTGTTCCGGTGGC+CGG | 0.509562 | 8.3:-61917397 | MS.gene57521:CDS |
| TGCAAGGTTCATAACAATAG+AGG | 0.521084 | 8.3:-61917647 | MS.gene57521:CDS |
| GGTAGTAGTAGTAATAGTGT+TGG | 0.528187 | 8.3:-61917607 | MS.gene57521:CDS |
| TGTTGGTATGGCTAGAATAC+CGG | 0.531504 | 8.3:-61917590 | MS.gene57521:CDS |
| CAGGAGCCATTACCGGCCAC+CGG | 0.541843 | 8.3:+61917385 | None:intergenic |
| AAGGCGGAAGTTACCCTACA+TGG | 0.551053 | 8.3:-61917702 | MS.gene57521:CDS |
| CCCTACATGGAACGAGAAGT+TGG | 0.557629 | 8.3:-61917689 | MS.gene57521:CDS |
| TCCGGTAACTGGTGTTCCGG+TGG | 0.577500 | 8.3:-61917401 | MS.gene57521:CDS |
| GGTTGTAAATATTTCAGTGA+AGG | 0.581610 | 8.3:-61917479 | MS.gene57521:CDS |
| CCTCCAAGAAAATCGGAAGC+CGG | 0.596810 | 8.3:+61917571 | None:intergenic |
| ACCAACTTCTCGTTCCATGT+AGG | 0.602619 | 8.3:+61917688 | None:intergenic |
| TTTGTCACGGTTCAATGCGA+CGG | 0.618568 | 8.3:-61917763 | MS.gene57521:CDS |
| GAGGTGAAGTACAAAACAAG+AGG | 0.620322 | 8.3:-61917628 | MS.gene57521:CDS |
| ACGAAACTTGATTCCGAAGG+CGG | 0.624950 | 8.3:-61917718 | MS.gene57521:CDS |
| AAAAGGGAACACATTTGTCA+CGG | 0.633602 | 8.3:-61917776 | MS.gene57521:CDS |
| TAATAGAGTTAGGAGAAATG+GGG | 0.635926 | 8.3:-61917500 | MS.gene57521:CDS |
| TCGACGTGCCATTACATGCA+AGG | 0.639724 | 8.3:-61917663 | MS.gene57521:CDS |
| AATTCGATGTCATCGATGAG+TGG | 0.641107 | 8.3:-61917427 | MS.gene57521:CDS |
| ACCACGAAACTTGATTCCGA+AGG | 0.642396 | 8.3:-61917721 | MS.gene57521:CDS |
| AATCTCCAAGAGAACAACAA+AGG | 0.671218 | 8.3:-61917802 | MS.gene57521:CDS |
| CCTGCAGCAGAGTTGCAACA+GGG | 0.674047 | 8.3:-61917366 | MS.gene57521:CDS |
| TCAATGCGACGGAAGCAACG+AGG | 0.728809 | 8.3:-61917752 | MS.gene57521:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGCAATTTTTAAGTTAT+AGG | - | chr8.3:61917637-61917656 | MS.gene57521:CDS | 15.0% |
| !!! | AATTTTTAAGTTATAGGTTG+TGG | - | chr8.3:61917643-61917662 | MS.gene57521:CDS | 20.0% |
| !!! | ATTTTTAAGTTATAGGTTGT+GGG | - | chr8.3:61917644-61917663 | MS.gene57521:CDS | 20.0% |
| ! | AATTAGAACAAGAACTTTGT+TGG | + | chr8.3:61917725-61917744 | None:intergenic | 25.0% |
| ! | ATAATAGAGTTAGGAGAAAT+GGG | - | chr8.3:61917667-61917686 | MS.gene57521:CDS | 25.0% |
| ! | ATTAGAACAAGAACTTTGTT+GGG | + | chr8.3:61917724-61917743 | None:intergenic | 25.0% |
| !! | AGAGATAACTATTTTATCAG+CGG | - | chr8.3:61917341-61917360 | MS.gene57521:CDS | 25.0% |
| GATAATAGAGTTAGGAGAAA+TGG | - | chr8.3:61917666-61917685 | MS.gene57521:CDS | 30.0% | |
| GGTTGTAAATATTTCAGTGA+AGG | - | chr8.3:61917689-61917708 | MS.gene57521:CDS | 30.0% | |
| TAATAGAGTTAGGAGAAATG+GGG | - | chr8.3:61917668-61917687 | MS.gene57521:CDS | 30.0% | |
| TACATAACCTCCAAGAAAAT+CGG | + | chr8.3:61917607-61917626 | None:intergenic | 30.0% | |
| ! | AGAACAACAAAGGCATAAAA+GGG | - | chr8.3:61917376-61917395 | MS.gene57521:CDS | 30.0% |
| !! | TAGTAGTAATAGTGTTGGTA+TGG | - | chr8.3:61917566-61917585 | MS.gene57521:CDS | 30.0% |
| AAAAGGGAACACATTTGTCA+CGG | - | chr8.3:61917392-61917411 | MS.gene57521:CDS | 35.0% | |
| AATCTCCAAGAGAACAACAA+AGG | - | chr8.3:61917366-61917385 | MS.gene57521:CDS | 35.0% | |
| GGTAGTAGTAGTAATAGTGT+TGG | - | chr8.3:61917561-61917580 | MS.gene57521:CDS | 35.0% | |
| GTTATGAACCTTGCATGTAA+TGG | + | chr8.3:61917516-61917535 | None:intergenic | 35.0% | |
| TGCAAGGTTCATAACAATAG+AGG | - | chr8.3:61917521-61917540 | MS.gene57521:CDS | 35.0% | |
| ! | GAGAACAACAAAGGCATAAA+AGG | - | chr8.3:61917375-61917394 | MS.gene57521:CDS | 35.0% |
| !! | TTATGCCTTTGTTGTTCTCT+TGG | + | chr8.3:61917374-61917393 | None:intergenic | 35.0% |
| AATTCGATGTCATCGATGAG+TGG | - | chr8.3:61917741-61917760 | MS.gene57521:CDS | 40.0% | |
| GAGGTGAAGTACAAAACAAG+AGG | - | chr8.3:61917540-61917559 | MS.gene57521:CDS | 40.0% | |
| GGTTGTGGGATAATAGAGTT+AGG | - | chr8.3:61917658-61917677 | MS.gene57521:CDS | 40.0% | |
| TGGTAGTTTAACCAAACAGC+AGG | + | chr8.3:61917831-61917850 | None:intergenic | 40.0% | |
| TGTTGGTATGGCTAGAATAC+CGG | - | chr8.3:61917578-61917597 | MS.gene57521:CDS | 40.0% | |
| ACCAACTTCTCGTTCCATGT+AGG | + | chr8.3:61917483-61917502 | None:intergenic | 45.0% | |
| ACCACGAAACTTGATTCCGA+AGG | - | chr8.3:61917447-61917466 | MS.gene57521:CDS | 45.0% | |
| ACGAAACTTGATTCCGAAGG+CGG | - | chr8.3:61917450-61917469 | MS.gene57521:CDS | 45.0% | |
| CCAACTTCTCGTTCCATGTA+GGG | + | chr8.3:61917482-61917501 | None:intergenic | 45.0% | |
| TTTGTCACGGTTCAATGCGA+CGG | - | chr8.3:61917405-61917424 | MS.gene57521:CDS | 45.0% | |
| !!! | ATACCGGCTTCCGATTTTCT+TGG | - | chr8.3:61917594-61917613 | MS.gene57521:CDS | 45.0% |
| AAGGCGGAAGTTACCCTACA+TGG | - | chr8.3:61917466-61917485 | MS.gene57521:CDS | 50.0% | |
| AGTTCCGGTAACTGGTGTTC+CGG | - | chr8.3:61917764-61917783 | MS.gene57521:CDS | 50.0% | |
| ATGAGTGGAGTTCCGGTAAC+TGG | - | chr8.3:61917756-61917775 | MS.gene57521:CDS | 50.0% | |
| CAACAGGGATTCCTGCTGTT+TGG | - | chr8.3:61917817-61917836 | MS.gene57521:CDS | 50.0% | |
| CCCTACATGGAACGAGAAGT+TGG | - | chr8.3:61917479-61917498 | MS.gene57521:CDS | 50.0% | |
| CCTCCAAGAAAATCGGAAGC+CGG | + | chr8.3:61917600-61917619 | None:intergenic | 50.0% | |
| GCCTTCGGAATCAAGTTTCG+TGG | + | chr8.3:61917451-61917470 | None:intergenic | 50.0% | |
| GTCATCGATGAGTGGAGTTC+CGG | - | chr8.3:61917749-61917768 | MS.gene57521:CDS | 50.0% | |
| TGTAGGGTAACTTCCGCCTT+CGG | + | chr8.3:61917466-61917485 | None:intergenic | 50.0% | |
| ! | TCGACGTGCCATTACATGCA+AGG | - | chr8.3:61917505-61917524 | MS.gene57521:CDS | 50.0% |
| CCTGCAGCAGAGTTGCAACA+GGG | - | chr8.3:61917802-61917821 | MS.gene57521:CDS | 55.0% | |
| TCAATGCGACGGAAGCAACG+AGG | - | chr8.3:61917416-61917435 | MS.gene57521:CDS | 55.0% | |
| TCCTGCAGCAGAGTTGCAAC+AGG | - | chr8.3:61917801-61917820 | MS.gene57521:CDS | 55.0% | |
| TCTGCTGCAGGAGCCATTAC+CGG | + | chr8.3:61917793-61917812 | None:intergenic | 55.0% | |
| ! | CCGGCTTCCGATTTTCTTGG+AGG | - | chr8.3:61917597-61917616 | MS.gene57521:CDS | 55.0% |
| CCCTGTTGCAACTCTGCTGC+AGG | + | chr8.3:61917805-61917824 | None:intergenic | 60.0% | |
| GCCACCGGAACACCAGTTAC+CGG | + | chr8.3:61917771-61917790 | None:intergenic | 60.0% | |
| GTAACTGGTGTTCCGGTGGC+CGG | - | chr8.3:61917771-61917790 | MS.gene57521:CDS | 60.0% | |
| TCCGGTAACTGGTGTTCCGG+TGG | - | chr8.3:61917767-61917786 | MS.gene57521:CDS | 60.0% | |
| CAGGAGCCATTACCGGCCAC+CGG | + | chr8.3:61917786-61917805 | None:intergenic | 65.0% | |
| GGTGTTCCGGTGGCCGGTAA+TGG | - | chr8.3:61917777-61917796 | MS.gene57521:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 61917327 | 61917863 | 61917327 | ID=MS.gene57521 |
| chr8.3 | mRNA | 61917327 | 61917863 | 61917327 | ID=MS.gene57521.t1;Parent=MS.gene57521 |
| chr8.3 | exon | 61917327 | 61917863 | 61917327 | ID=MS.gene57521.t1.exon1;Parent=MS.gene57521.t1 |
| chr8.3 | CDS | 61917327 | 61917863 | 61917327 | ID=cds.MS.gene57521.t1;Parent=MS.gene57521.t1 |
| Gene Sequence |
| Protein sequence |
