Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57754.t1 | XP_013458136.1 | 98.9 | 94 | 1 | 0 | 1 | 94 | 1 | 94 | 3.60E-47 | 197.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57754.t1 | O80786 | 64.9 | 94 | 29 | 1 | 1 | 94 | 1 | 90 | 1.9e-25 | 116.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57754.t1 | A0A072UR54 | 98.9 | 94 | 1 | 0 | 1 | 94 | 1 | 94 | 2.6e-47 | 197.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049397 | MS.gene57754 | 0.800195 | 1.62E-48 | -1.69E-46 |
| MS.gene049622 | MS.gene57754 | 0.803841 | 2.88E-49 | -1.69E-46 |
| MS.gene049989 | MS.gene57754 | 0.821204 | 4.57E-53 | -1.69E-46 |
| MS.gene050297 | MS.gene57754 | 0.818805 | 1.62E-52 | -1.69E-46 |
| MS.gene052367 | MS.gene57754 | 0.800322 | 1.52E-48 | -1.69E-46 |
| MS.gene060565 | MS.gene57754 | 0.810858 | 9.35E-51 | -1.69E-46 |
| MS.gene061260 | MS.gene57754 | 0.801316 | 9.54E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57754.t1 | MTR_4g116870 | 98.936 | 94 | 1 | 0 | 1 | 94 | 1 | 94 | 1.30e-64 | 207 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57754.t1 | AT2G07690 | 64.894 | 94 | 29 | 1 | 1 | 94 | 1 | 90 | 4.79e-34 | 123 |
| MS.gene57754.t1 | AT2G07690 | 64.894 | 94 | 29 | 1 | 1 | 94 | 1 | 90 | 5.22e-34 | 123 |
Find 0 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATATCAACGAGAAGATATTT+GGG | + | 32402:771-790 | MS.gene57754:intergenic | 25.0% |
| !! | GGATGAATTCTTTGAATTTT+TGG | + | 32402:692-711 | MS.gene57754:intergenic | 25.0% |
| !!! | TGAATTCTTTGAATTTTTGG+AGG | + | 32402:689-708 | MS.gene57754:intergenic | 25.0% |
| AATTACCAGAGGAAGAATAT+CGG | + | 32402:850-869 | MS.gene57753:intergenic | 30.0% | |
| AGTTTAGAAGAAATTACCAG+AGG | + | 32402:861-880 | MS.gene57754:intergenic | 30.0% | |
| CAAATATCTTCTCGTTGATA+TGG | - | 32402:770-789 | MS.gene57754:CDS | 30.0% | |
| CATATCAACGAGAAGATATT+TGG | + | 32402:772-791 | MS.gene57754:intergenic | 30.0% | |
| !! | TTCTTTGAATTTTTGGAGGA+TGG | + | 32402:685-704 | MS.gene57754:intergenic | 30.0% |
| GTAAGGAAAGACATTGTTAC+CGG | + | 32402:727-746 | MS.gene57754:intergenic | 35.0% | |
| ! | GATATTTGGGATTGTGAAGA+AGG | + | 32402:758-777 | MS.gene57754:intergenic | 35.0% |
| !! | TGTTACCGGTTTCAAAGTTA+CGG | + | 32402:713-732 | MS.gene57754:intergenic | 35.0% |
| !! | TTCATCCGTAACTTTGAAAC+CGG | - | 32402:705-724 | MS.gene57754:CDS | 35.0% |
| GGAGTTTAGAAGGAAGATCA+GGG | + | 32402:815-834 | MS.gene57754:intergenic | 40.0% | |
| TGAAGAAGGCTTTCTCTGTA+AGG | + | 32402:744-763 | MS.gene57754:intergenic | 40.0% | |
| !! | TGAATTTTTGGAGGATGGTG+TGG | + | 32402:680-699 | MS.gene57754:intergenic | 40.0% |
| CGGAGTTTAGAAGGAAGATC+AGG | + | 32402:816-835 | MS.gene57754:intergenic | 45.0% | |
| !! | TTTTTGGAGGATGGTGTGGT+TGG | + | 32402:676-695 | MS.gene57754:intergenic | 45.0% |
| ACCAGAGGAAGAATATCGGC+AGG | + | 32402:846-865 | MS.gene57754:intergenic | 50.0% | |
| GAATATCGGCAGGAGCAGAT+CGG | + | 32402:836-855 | MS.gene57754:intergenic | 50.0% | |
| GGAGCAGATCGGAGTTTAGA+AGG | + | 32402:825-844 | MS.gene57754:intergenic | 50.0% | |
| TCCTGCCGATATTCTTCCTC+TGG | - | 32402:842-861 | MS.gene57754:CDS | 50.0% | |
| ACAGCGACCAAGCACACTCA+TGG | - | 32402:613-632 | MS.gene57754:CDS | 55.0% | |
| CAAGCACACTCATGGGACGA+CGG | - | 32402:621-640 | MS.gene57754:CDS | 55.0% | |
| CAGCGACCAAGCACACTCAT+GGG | - | 32402:614-633 | MS.gene57754:CDS | 55.0% | |
| GAGGATGGTGTGGTTGGAGA+TGG | + | 32402:670-689 | MS.gene57754:intergenic | 55.0% | |
| GATGGTGTGGTTGGAGATGG+TGG | + | 32402:667-686 | MS.gene57754:intergenic | 55.0% | |
| ! | GTGGTTGGAGATGGTGGCAT+CGG | + | 32402:661-680 | MS.gene57754:intergenic | 55.0% |
| !! | ATGTCAGGTTGGGACGAAGG+AGG | - | 32402:582-601 | MS.gene57754:CDS | 55.0% |
| CGTCGTCCCATGAGTGTGCT+TGG | + | 32402:623-642 | MS.gene57754:intergenic | 60.0% | |
| !! | TGGCATCGGTTTCGCCGCCA+CGG | + | 32402:647-666 | MS.gene57754:intergenic | 65.0% |
| CACTCATGGGACGACGGCCG+TGG | - | 32402:627-646 | MS.gene57754:CDS | 70.0% | |
| TCATGGGACGACGGCCGTGG+CGG | - | 32402:630-649 | MS.gene57754:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32402 | gene | 582 | 884 | 582 | ID=MS.gene57754 |
| 32402 | mRNA | 582 | 884 | 582 | ID=MS.gene57754.t1;Parent=MS.gene57754 |
| 32402 | exon | 582 | 884 | 582 | ID=MS.gene57754.t1.exon1;Parent=MS.gene57754.t1 |
| 32402 | CDS | 582 | 884 | 582 | ID=cds.MS.gene57754.t1;Parent=MS.gene57754.t1 |
| Gene Sequence |
| Protein sequence |
