AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049622


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049622.t1 MTR_3g063110 98.667 150 2 0 1 150 1 150 1.01e-103 293
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049622.t1 AT3G46940 87.943 141 17 0 10 150 26 166 5.46e-87 251
MS.gene049622.t1 AT3G46940 87.943 141 17 0 10 150 67 207 6.22e-87 253

Find 47 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCGGAAGTTTCTGAGGTTT+TGG 0.225618 3.2:+59167932 MS.gene049622:CDS
GTTAGAGGTGAAGGTGGTTT+TGG 0.293840 3.2:+59167969 MS.gene049622:CDS
GATTTGGATTCTACTGTTAG+AGG 0.343621 3.2:+59167954 MS.gene049622:CDS
TTTCTTCACTCGGAGAAAAC+TGG 0.351836 3.2:-59167503 None:intergenic
TCAACAATGTCTAACTTAAA+CGG 0.360985 3.2:+59167469 None:intergenic
ACACGAGCATAGGTTCCTTC+AGG 0.369361 3.2:-59167658 None:intergenic
AACCTTTGAATCGGCGGCGC+TGG 0.374420 3.2:-59167587 None:intergenic
CTGATTACAGAGGACCTGTT+GGG 0.415838 3.2:+59167823 MS.gene049622:CDS
CATTCGATTGACGTTGGTGC+TGG 0.432456 3.2:+59167789 MS.gene049622:CDS
TCCAAAACCTCAGAAACTTC+CGG 0.440429 3.2:-59167933 None:intergenic
CGCGGCTCCCCTCTCTCTGC+CGG 0.443450 3.2:+59167553 MS.gene049622:CDS
ATGAGAGCTTTGCCGCGAGC+AGG 0.461473 3.2:-59167610 None:intergenic
CGCCGAGATCGGGGTTGACA+TGG 0.473074 3.2:+59167763 MS.gene049622:CDS
GGCAGAGAGAGGGGAGCCGC+GGG 0.476034 3.2:-59167551 None:intergenic
TTCCATGTCAACCCCGATCT+CGG 0.481839 3.2:-59167765 None:intergenic
GGAAGGTATGACGGCTTTGT+CGG 0.486471 3.2:-59167530 None:intergenic
TTAAACAGTATAACCCCAAC+AGG 0.497604 3.2:-59167837 None:intergenic
GATTCTACTGTTAGAGGTGA+AGG 0.505946 3.2:+59167960 MS.gene049622:CDS
TGTCGGAGAGTTTCTTCACT+CGG 0.507441 3.2:-59167513 None:intergenic
CTCCAGCGCCGCCGATTCAA+AGG 0.509909 3.2:+59167585 MS.gene049622:CDS
CTCTCCATCGCCGTCCCTGA+AGG 0.513836 3.2:+59167643 MS.gene049622:CDS
GGCGCTGGAGAGATCGTATC+CGG 0.519854 3.2:-59167572 None:intergenic
GAGAGAGGGGAGCCGCGGGA+AGG 0.523039 3.2:-59167547 None:intergenic
CACGAGCATAGGTTCCTTCA+GGG 0.535140 3.2:-59167657 None:intergenic
TATTGAGAAGATTATTACTC+CGG 0.540579 3.2:+59167914 MS.gene049622:CDS
GCGAGCAGGAACCTTTGAAT+CGG 0.540643 3.2:-59167596 None:intergenic
GATCGTATCCGGCAGAGAGA+GGG 0.549237 3.2:-59167561 None:intergenic
CGGCAGAGAGAGGGGAGCCG+CGG 0.551999 3.2:-59167552 None:intergenic
ACGGCGATGGAGAGATCTGT+GGG 0.554559 3.2:-59167634 None:intergenic
GGAGCCGCGGGAAGGTATGA+CGG 0.557254 3.2:-59167539 None:intergenic
ATCGTATCCGGCAGAGAGAG+GGG 0.558090 3.2:-59167560 None:intergenic
GGTTCCTTCAGGGACGGCGA+TGG 0.559917 3.2:-59167647 None:intergenic
TATTACTCCGGAAGTTTCTG+AGG 0.562326 3.2:+59167926 MS.gene049622:CDS
TCTACTGTTAGAGGTGAAGG+TGG 0.578300 3.2:+59167963 MS.gene049622:CDS
GACGGCGATGGAGAGATCTG+TGG 0.578622 3.2:-59167635 None:intergenic
AGCAGGAACCTTTGAATCGG+CGG 0.581168 3.2:-59167593 None:intergenic
AGATCGTATCCGGCAGAGAG+AGG 0.583505 3.2:-59167562 None:intergenic
GTAATTACCAACACGAGCAT+AGG 0.588762 3.2:-59167668 None:intergenic
GAAGGAACCTATGCTCGTGT+TGG 0.606547 3.2:+59167661 MS.gene049622:CDS
GCTGATTACAGAGGACCTGT+TGG 0.614644 3.2:+59167822 MS.gene049622:CDS
TGGAAGCATTCGATTGACGT+TGG 0.614719 3.2:+59167783 MS.gene049622:CDS
AGCATAGGTTCCTTCAGGGA+CGG 0.629807 3.2:-59167653 None:intergenic
GATTCAAAGGTTCCTGCTCG+CGG 0.632457 3.2:+59167598 MS.gene049622:CDS
TGATTACAGAGGACCTGTTG+GGG 0.654205 3.2:+59167824 MS.gene049622:CDS
AAAGCCGTCATACCTTCCCG+CGG 0.718692 3.2:+59167535 MS.gene049622:CDS
GTGATTGATGCTGATTACAG+AGG 0.740502 3.2:+59167813 MS.gene049622:CDS
CGGCGATGGAGAGATCTGTG+GGG 0.756952 3.2:-59167633 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAGGGTTTTGAAATTT+AGG + chr3.2:59167710-59167729 MS.gene049622:intron 20.0%
!!! TTATAGGGTTTTGAAATTTA+GGG + chr3.2:59167711-59167730 MS.gene049622:intron 20.0%
! AAAACCCTATAATACAAATC+AGG - chr3.2:59167703-59167722 None:intergenic 25.0%
! AAACCCTATAATACAAATCA+GGG - chr3.2:59167702-59167721 None:intergenic 25.0%
! TATTGAGAAGATTATTACTC+CGG + chr3.2:59167914-59167933 MS.gene049622:CDS 25.0%
AACCCTATAATACAAATCAG+GGG - chr3.2:59167701-59167720 None:intergenic 30.0%
!!! GTTGATTTTGAAGTGAAAGT+TGG + chr3.2:59167870-59167889 MS.gene049622:CDS 30.0%
ACCCTATAATACAAATCAGG+GGG - chr3.2:59167700-59167719 None:intergenic 35.0%
GATTTGGATTCTACTGTTAG+AGG + chr3.2:59167954-59167973 MS.gene049622:CDS 35.0%
TTAAACAGTATAACCCCAAC+AGG - chr3.2:59167840-59167859 None:intergenic 35.0%
!!! AGTTTCTGAGGTTTTGGATT+TGG + chr3.2:59167938-59167957 MS.gene049622:CDS 35.0%
CCCTATAATACAAATCAGGG+GGG - chr3.2:59167699-59167718 None:intergenic 40.0%
GATTCTACTGTTAGAGGTGA+AGG + chr3.2:59167960-59167979 MS.gene049622:CDS 40.0%
GTAATTACCAACACGAGCAT+AGG - chr3.2:59167671-59167690 None:intergenic 40.0%
TATTACTCCGGAAGTTTCTG+AGG + chr3.2:59167926-59167945 MS.gene049622:CDS 40.0%
TCCAAAACCTCAGAAACTTC+CGG - chr3.2:59167936-59167955 None:intergenic 40.0%
TTTCTTCACTCGGAGAAAAC+TGG - chr3.2:59167506-59167525 None:intergenic 40.0%
! CCCCCCTGATTTGTATTATA+GGG + chr3.2:59167696-59167715 MS.gene049622:intron 40.0%
! GTGATTGATGCTGATTACAG+AGG + chr3.2:59167813-59167832 MS.gene049622:CDS 40.0%
! TCCCCCCTGATTTGTATTAT+AGG + chr3.2:59167695-59167714 MS.gene049622:intron 40.0%
!!! AAGGTGGTTTTGGATCTACT+GGG + chr3.2:59167979-59167998 MS.gene049622:CDS 40.0%
CTGATTACAGAGGACCTGTT+GGG + chr3.2:59167823-59167842 MS.gene049622:CDS 45.0%
TCTACTGTTAGAGGTGAAGG+TGG + chr3.2:59167963-59167982 MS.gene049622:CDS 45.0%
TGATTACAGAGGACCTGTTG+GGG + chr3.2:59167824-59167843 MS.gene049622:CDS 45.0%
TGTCGGAGAGTTTCTTCACT+CGG - chr3.2:59167516-59167535 None:intergenic 45.0%
! GTTAGAGGTGAAGGTGGTTT+TGG + chr3.2:59167969-59167988 MS.gene049622:CDS 45.0%
!! TGGAAGCATTCGATTGACGT+TGG + chr3.2:59167783-59167802 MS.gene049622:CDS 45.0%
!!! AGGTGGTTTTGGATCTACTG+GGG + chr3.2:59167980-59167999 MS.gene049622:CDS 45.0%
!!! GAAGGTGGTTTTGGATCTAC+TGG + chr3.2:59167978-59167997 MS.gene049622:CDS 45.0%
!!! TCCGGAAGTTTCTGAGGTTT+TGG + chr3.2:59167932-59167951 MS.gene049622:CDS 45.0%
ACACGAGCATAGGTTCCTTC+AGG - chr3.2:59167661-59167680 None:intergenic 50.0%
AGCAGGAACCTTTGAATCGG+CGG - chr3.2:59167596-59167615 None:intergenic 50.0%
AGCATAGGTTCCTTCAGGGA+CGG - chr3.2:59167656-59167675 None:intergenic 50.0%
CACGAGCATAGGTTCCTTCA+GGG - chr3.2:59167660-59167679 None:intergenic 50.0%
CATTCGATTGACGTTGGTGC+TGG + chr3.2:59167789-59167808 MS.gene049622:CDS 50.0%
GAAGGAACCTATGCTCGTGT+TGG + chr3.2:59167661-59167680 MS.gene049622:CDS 50.0%
GATTCAAAGGTTCCTGCTCG+CGG + chr3.2:59167598-59167617 MS.gene049622:CDS 50.0%
GCGAGCAGGAACCTTTGAAT+CGG - chr3.2:59167599-59167618 None:intergenic 50.0%
GCTGATTACAGAGGACCTGT+TGG + chr3.2:59167822-59167841 MS.gene049622:CDS 50.0%
TTCCATGTCAACCCCGATCT+CGG - chr3.2:59167768-59167787 None:intergenic 50.0%
! GGAAGGTATGACGGCTTTGT+CGG - chr3.2:59167533-59167552 None:intergenic 50.0%
!! ATTTTTGCAGCGCCGAGATC+GGG + chr3.2:59167753-59167772 MS.gene049622:intron 50.0%
!! GATTTTTGCAGCGCCGAGAT+CGG + chr3.2:59167752-59167771 MS.gene049622:intron 50.0%
AAAGCCGTCATACCTTCCCG+CGG + chr3.2:59167535-59167554 MS.gene049622:CDS 55.0%
ACGGCGATGGAGAGATCTGT+GGG - chr3.2:59167637-59167656 None:intergenic 55.0%
AGATCGTATCCGGCAGAGAG+AGG - chr3.2:59167565-59167584 None:intergenic 55.0%
ATCGTATCCGGCAGAGAGAG+GGG - chr3.2:59167563-59167582 None:intergenic 55.0%
GATCGTATCCGGCAGAGAGA+GGG - chr3.2:59167564-59167583 None:intergenic 55.0%
!! TTTTTGCAGCGCCGAGATCG+GGG + chr3.2:59167754-59167773 MS.gene049622:intron 55.0%
AACCTTTGAATCGGCGGCGC+TGG - chr3.2:59167590-59167609 None:intergenic 60.0%
ATGAGAGCTTTGCCGCGAGC+AGG - chr3.2:59167613-59167632 None:intergenic 60.0%
CGGCGATGGAGAGATCTGTG+GGG - chr3.2:59167636-59167655 None:intergenic 60.0%
GACGGCGATGGAGAGATCTG+TGG - chr3.2:59167638-59167657 None:intergenic 60.0%
! GGCGCTGGAGAGATCGTATC+CGG - chr3.2:59167575-59167594 None:intergenic 60.0%
CGCCGAGATCGGGGTTGACA+TGG + chr3.2:59167763-59167782 MS.gene049622:CDS 65.0%
CTCTCCATCGCCGTCCCTGA+AGG + chr3.2:59167643-59167662 MS.gene049622:CDS 65.0%
GGTTCCTTCAGGGACGGCGA+TGG - chr3.2:59167650-59167669 None:intergenic 65.0%
! GGAGCCGCGGGAAGGTATGA+CGG - chr3.2:59167542-59167561 None:intergenic 65.0%
!! CTCCAGCGCCGCCGATTCAA+AGG + chr3.2:59167585-59167604 MS.gene049622:CDS 65.0%
CGCGGCTCCCCTCTCTCTGC+CGG + chr3.2:59167553-59167572 MS.gene049622:CDS 75.0%
CGGCAGAGAGAGGGGAGCCG+CGG - chr3.2:59167555-59167574 None:intergenic 75.0%
GAGAGAGGGGAGCCGCGGGA+AGG - chr3.2:59167550-59167569 None:intergenic 75.0%
GGCAGAGAGAGGGGAGCCGC+GGG - chr3.2:59167554-59167573 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 59167475 59168007 59167475 ID=MS.gene049622
chr3.2 mRNA 59167475 59168007 59167475 ID=MS.gene049622.t1;Parent=MS.gene049622
chr3.2 exon 59167475 59167682 59167475 ID=MS.gene049622.t1.exon1;Parent=MS.gene049622.t1
chr3.2 CDS 59167475 59167682 59167475 ID=cds.MS.gene049622.t1;Parent=MS.gene049622.t1
chr3.2 exon 59167763 59168007 59167763 ID=MS.gene049622.t1.exon2;Parent=MS.gene049622.t1
chr3.2 CDS 59167763 59168007 59167763 ID=cds.MS.gene049622.t1;Parent=MS.gene049622.t1
Gene Sequence

>MS.gene049622

ATGTCTAACTTAAACGGCGTCGTTTCATCCAGTTTTCTCCGAGTGAAGAAACTCTCCGACAAAGCCGTCATACCTTCCCGCGGCTCCCCTCTCTCTGCCGGATACGATCTCTCCAGCGCCGCCGATTCAAAGGTTCCTGCTCGCGGCAAAGCTCTCATCCCCACAGATCTCTCCATCGCCGTCCCTGAAGGAACCTATGCTCGTGTTGGTAATTACTATTTCCCCCCTGATTTGTATTATAGGGTTTTGAAATTTAGGGTTTTTGATTGAATTGAATGATTTTTGCAGCGCCGAGATCGGGGTTGACATGGAAGCATTCGATTGACGTTGGTGCTGGAGTGATTGATGCTGATTACAGAGGACCTGTTGGGGTTATACTGTTTAATCATTCTGATGTTGATTTTGAAGTGAAAGTTGGTGATAGAGTTGCTCAGTTGATTATTGAGAAGATTATTACTCCGGAAGTTTCTGAGGTTTTGGATTTGGATTCTACTGTTAGAGGTGAAGGTGGTTTTGGATCTACTGGGGTTTGA

Protein sequence

>MS.gene049622.t1

MSNLNGVVSSSFLRVKKLSDKAVIPSRGSPLSAGYDLSSAADSKVPARGKALIPTDLSIAVPEGTYARVAPRSGLTWKHSIDVGAGVIDADYRGPVGVILFNHSDVDFEVKVGDRVAQLIIEKIITPEVSEVLDLDSTVRGEGGFGSTGV