Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57757.t1 | XP_013448012.1 | 95.5 | 176 | 8 | 0 | 1 | 176 | 20 | 195 | 1.20E-93 | 352.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57757.t1 | Q9LI89 | 48.3 | 176 | 79 | 2 | 12 | 175 | 34 | 209 | 1.8e-37 | 157.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57757.t1 | A0A072TX07 | 95.5 | 176 | 8 | 0 | 1 | 176 | 20 | 195 | 8.5e-94 | 352.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049281 | MS.gene57757 | 0.836269 | 1.03E-56 | -1.69E-46 |
| MS.gene051973 | MS.gene57757 | 0.810616 | 1.06E-50 | -1.69E-46 |
| MS.gene05470 | MS.gene57757 | -0.807423 | 5.09E-50 | -1.69E-46 |
| MS.gene054959 | MS.gene57757 | 0.82937 | 5.35E-55 | -1.69E-46 |
| MS.gene055502 | MS.gene57757 | -0.803769 | 2.98E-49 | -1.69E-46 |
| MS.gene056748 | MS.gene57757 | 0.826793 | 2.23E-54 | -1.69E-46 |
| MS.gene056988 | MS.gene57757 | 0.825567 | 4.37E-54 | -1.69E-46 |
| MS.gene057261 | MS.gene57757 | 0.8166 | 5.09E-52 | -1.69E-46 |
| MS.gene058544 | MS.gene57757 | -0.820294 | 7.40E-53 | -1.69E-46 |
| MS.gene06077 | MS.gene57757 | -0.804711 | 1.89E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTTGACGGACCTGTTATT+TGG | 0.224264 | 7.4:+72162706 | MS.gene57757:CDS |
| TCAGATTACAGCTTTGAATT+TGG | 0.229608 | 7.4:+72162633 | MS.gene57757:CDS |
| GGGTCAGAGTTATTTGTTTC+GGG | 0.243484 | 7.4:+72162678 | MS.gene57757:CDS |
| AGTTTCCAATGTTGCGATCT+TGG | 0.284283 | 7.4:-72162438 | None:intergenic |
| CTATTTGCTTCAGCTAGTTC+TGG | 0.294486 | 7.4:+72162789 | MS.gene57757:CDS |
| GCGGCGAGATCTACAAAATA+AGG | 0.302206 | 7.4:+72162499 | MS.gene57757:CDS |
| CAGAACTAGCTGAAGCAAAT+AGG | 0.366562 | 7.4:-72162788 | None:intergenic |
| AGGGTCAGAGTTATTTGTTT+CGG | 0.390691 | 7.4:+72162677 | MS.gene57757:CDS |
| CTGTAATCTGATTGAATCCT+TGG | 0.399975 | 7.4:-72162621 | None:intergenic |
| GAATCATATCGCCAAATAAC+AGG | 0.403217 | 7.4:-72162717 | None:intergenic |
| TGTTATTTGGCGATATGATT+CGG | 0.418939 | 7.4:+72162719 | MS.gene57757:CDS |
| CGAAAGCAATTGGTGGGGTA+TGG | 0.427240 | 7.4:+72162569 | MS.gene57757:CDS |
| TACAAAATAAGGAAAGAGTT+AGG | 0.428564 | 7.4:+72162510 | MS.gene57757:CDS |
| TAGCTAGCGGCGAAAGCAAT+TGG | 0.476177 | 7.4:+72162559 | MS.gene57757:CDS |
| AGCTTCCTGCTTGATTTGAA+AGG | 0.476416 | 7.4:-72162597 | None:intergenic |
| CGAGCTAGAGACATCGATCT+TGG | 0.476661 | 7.4:+72162407 | MS.gene57757:CDS |
| AATCAAGCAGGAAGCTTCCA+AGG | 0.494763 | 7.4:+72162604 | MS.gene57757:CDS |
| GATTTCCAAGATCGCAACAT+TGG | 0.509774 | 7.4:+72162433 | MS.gene57757:CDS |
| GAAAGAAGAATCTTTACCTC+AGG | 0.525784 | 7.4:+72162356 | MS.gene57757:CDS |
| GTTTCGGGTAAAGAATTTGA+CGG | 0.527787 | 7.4:+72162693 | MS.gene57757:CDS |
| GATACTAATGAATGGTTCAA+AGG | 0.541527 | 7.4:+72162741 | MS.gene57757:CDS |
| ATGATTCGGATACTAATGAA+TGG | 0.555866 | 7.4:+72162733 | MS.gene57757:CDS |
| TGAAGAACCGTTGCCATCAG+AGG | 0.562265 | 7.4:-72162331 | None:intergenic |
| GTCCGTTCATGATTAATCCT+AGG | 0.572154 | 7.4:+72162763 | MS.gene57757:CDS |
| GCAATTGGTGGGGTATGGAA+TGG | 0.578887 | 7.4:+72162574 | MS.gene57757:CDS |
| TAGCGGCGAAAGCAATTGGT+GGG | 0.579866 | 7.4:+72162563 | MS.gene57757:CDS |
| TTACCACCCTCTGATGGCAA+CGG | 0.580464 | 7.4:+72162324 | MS.gene57757:CDS |
| GAAGAACCGTTGCCATCAGA+GGG | 0.591725 | 7.4:-72162330 | None:intergenic |
| GACTTGTTACCACCCTCTGA+TGG | 0.609485 | 7.4:+72162318 | MS.gene57757:CDS |
| CACCTAGGATTAATCATGAA+CGG | 0.612535 | 7.4:-72162765 | None:intergenic |
| AATGGCCTTTCAAATCAAGC+AGG | 0.615937 | 7.4:+72162592 | MS.gene57757:CDS |
| CTAGCGGCGAAAGCAATTGG+TGG | 0.628757 | 7.4:+72162562 | MS.gene57757:CDS |
| GCTGAAGCAAATAGGCACCT+AGG | 0.638194 | 7.4:-72162780 | None:intergenic |
| AGCGGCGAAAGCAATTGGTG+GGG | 0.663533 | 7.4:+72162564 | MS.gene57757:CDS |
| AGATTATAATCTGCATCCTG+AGG | 0.665618 | 7.4:-72162372 | None:intergenic |
| GAACCGTTGCCATCAGAGGG+TGG | 0.673262 | 7.4:-72162327 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TACAAAATAAGGAAAGAGTT+AGG | + | chr7.4:72162510-72162529 | MS.gene57757:CDS | 25.0% |
| !! | ATAAAGAATCATTTTCAGCA+GGG | + | chr7.4:72162658-72162677 | MS.gene57757:CDS | 25.0% |
| GATACTAATGAATGGTTCAA+AGG | + | chr7.4:72162741-72162760 | MS.gene57757:CDS | 30.0% | |
| ! | ATGATTCGGATACTAATGAA+TGG | + | chr7.4:72162733-72162752 | MS.gene57757:CDS | 30.0% |
| ! | GATAAAGAATCATTTTCAGC+AGG | + | chr7.4:72162657-72162676 | MS.gene57757:CDS | 30.0% |
| ! | TGTTATTTGGCGATATGATT+CGG | + | chr7.4:72162719-72162738 | MS.gene57757:CDS | 30.0% |
| ! | TTTTTAACCCTGATGAAAAG+CGG | + | chr7.4:72162480-72162499 | MS.gene57757:CDS | 30.0% |
| !! | TCAGATTACAGCTTTGAATT+TGG | + | chr7.4:72162633-72162652 | MS.gene57757:CDS | 30.0% |
| AGATTATAATCTGCATCCTG+AGG | - | chr7.4:72162375-72162394 | None:intergenic | 35.0% | |
| AGGGTCAGAGTTATTTGTTT+CGG | + | chr7.4:72162677-72162696 | MS.gene57757:CDS | 35.0% | |
| CACCTAGGATTAATCATGAA+CGG | - | chr7.4:72162768-72162787 | None:intergenic | 35.0% | |
| CTAGCTAACATAAAAACGGA+CGG | - | chr7.4:72162546-72162565 | None:intergenic | 35.0% | |
| CTGTAATCTGATTGAATCCT+TGG | - | chr7.4:72162624-72162643 | None:intergenic | 35.0% | |
| GAAAGAAGAATCTTTACCTC+AGG | + | chr7.4:72162356-72162375 | MS.gene57757:CDS | 35.0% | |
| GAATCATATCGCCAAATAAC+AGG | - | chr7.4:72162720-72162739 | None:intergenic | 35.0% | |
| ! | AATTTGACGGACCTGTTATT+TGG | + | chr7.4:72162706-72162725 | MS.gene57757:CDS | 35.0% |
| ! | AGAATTCTCATCTTTTGTGC+TGG | - | chr7.4:72162297-72162316 | None:intergenic | 35.0% |
| ! | GTTTCGGGTAAAGAATTTGA+CGG | + | chr7.4:72162693-72162712 | MS.gene57757:CDS | 35.0% |
| ! | TCTTTTGTGCTGGAAAATTG+TGG | - | chr7.4:72162287-72162306 | None:intergenic | 35.0% |
| !! | TCCGTTTTTATGTTAGCTAG+CGG | + | chr7.4:72162546-72162565 | MS.gene57757:CDS | 35.0% |
| AATGGCCTTTCAAATCAAGC+AGG | + | chr7.4:72162592-72162611 | MS.gene57757:CDS | 40.0% | |
| AGTTTCCAATGTTGCGATCT+TGG | - | chr7.4:72162441-72162460 | None:intergenic | 40.0% | |
| CAGAACTAGCTGAAGCAAAT+AGG | - | chr7.4:72162791-72162810 | None:intergenic | 40.0% | |
| GATTTCCAAGATCGCAACAT+TGG | + | chr7.4:72162433-72162452 | MS.gene57757:CDS | 40.0% | |
| GCCGCTAGCTAACATAAAAA+CGG | - | chr7.4:72162550-72162569 | None:intergenic | 40.0% | |
| GGGTCAGAGTTATTTGTTTC+GGG | + | chr7.4:72162678-72162697 | MS.gene57757:CDS | 40.0% | |
| GTCCGTTCATGATTAATCCT+AGG | + | chr7.4:72162763-72162782 | MS.gene57757:CDS | 40.0% | |
| ! | AGCTTCCTGCTTGATTTGAA+AGG | - | chr7.4:72162600-72162619 | None:intergenic | 40.0% |
| AATCAAGCAGGAAGCTTCCA+AGG | + | chr7.4:72162604-72162623 | MS.gene57757:CDS | 45.0% | |
| GCGGCGAGATCTACAAAATA+AGG | + | chr7.4:72162499-72162518 | MS.gene57757:CDS | 45.0% | |
| CGAAAGCAATTGGTGGGGTA+TGG | + | chr7.4:72162569-72162588 | MS.gene57757:CDS | 50.0% | |
| CGAGCTAGAGACATCGATCT+TGG | + | chr7.4:72162407-72162426 | MS.gene57757:CDS | 50.0% | |
| GAAGAACCGTTGCCATCAGA+GGG | - | chr7.4:72162333-72162352 | None:intergenic | 50.0% | |
| GCAATTGGTGGGGTATGGAA+TGG | + | chr7.4:72162574-72162593 | MS.gene57757:CDS | 50.0% | |
| GCTGAAGCAAATAGGCACCT+AGG | - | chr7.4:72162783-72162802 | None:intergenic | 50.0% | |
| TAGCGGCGAAAGCAATTGGT+GGG | + | chr7.4:72162563-72162582 | MS.gene57757:CDS | 50.0% | |
| TAGCTAGCGGCGAAAGCAAT+TGG | + | chr7.4:72162559-72162578 | MS.gene57757:CDS | 50.0% | |
| TGAAGAACCGTTGCCATCAG+AGG | - | chr7.4:72162334-72162353 | None:intergenic | 50.0% | |
| TTACCACCCTCTGATGGCAA+CGG | + | chr7.4:72162324-72162343 | MS.gene57757:CDS | 50.0% | |
| !! | GACTTGTTACCACCCTCTGA+TGG | + | chr7.4:72162318-72162337 | MS.gene57757:CDS | 50.0% |
| !!! | AGATCTCGCCGCTTTTCATC+AGG | - | chr7.4:72162491-72162510 | None:intergenic | 50.0% |
| !!! | GATCTCGCCGCTTTTCATCA+GGG | - | chr7.4:72162490-72162509 | None:intergenic | 50.0% |
| AGCGGCGAAAGCAATTGGTG+GGG | + | chr7.4:72162564-72162583 | MS.gene57757:CDS | 55.0% | |
| CTAGCGGCGAAAGCAATTGG+TGG | + | chr7.4:72162562-72162581 | MS.gene57757:CDS | 55.0% | |
| GAACCGTTGCCATCAGAGGG+TGG | - | chr7.4:72162330-72162349 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 72162281 | 72162810 | 72162281 | ID=MS.gene57757 |
| chr7.4 | mRNA | 72162281 | 72162810 | 72162281 | ID=MS.gene57757.t1;Parent=MS.gene57757 |
| chr7.4 | exon | 72162281 | 72162810 | 72162281 | ID=MS.gene57757.t1.exon1;Parent=MS.gene57757.t1 |
| chr7.4 | CDS | 72162281 | 72162810 | 72162281 | ID=cds.MS.gene57757.t1;Parent=MS.gene57757.t1 |
| Gene Sequence |
| Protein sequence |
