AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058544


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058544.t1 MTR_7g080793 92.661 109 5 2 1 108 1 107 7.80e-71 206
MS.gene058544.t1 MTR_7g080803 69.091 110 30 3 1 108 1 108 1.33e-46 145
MS.gene058544.t1 MTR_5g008460 68.182 110 31 3 1 108 1 108 2.66e-46 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058544.t1 AT4G10040 68.182 110 31 3 1 108 1 108 5.45e-46 144
MS.gene058544.t1 AT1G22840 66.364 110 33 3 1 108 1 108 3.81e-44 139
MS.gene058544.t1 AT1G22840 63.636 88 29 3 1 86 1 87 2.17e-31 107

Find 0 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATAATCATAATAAATTAA+TGG - 50482:14673-14692 None:intergenic 10.0%
!! TAAAGAATATTTATATCAAA+TGG + 50482:14560-14579 MS.gene058544:intron 10.0%
!! TATTAAATGTATTTCATATT+AGG + 50482:14210-14229 MS.gene058544:intron 10.0%
!! ACTAGTATCATAATATTCTA+AGG + 50482:14324-14343 MS.gene058544:intron 20.0%
!! CTTTATAAAGAAAATACATG+TGG + 50482:13971-13990 MS.gene058544:intron 20.0%
!! GTAAATATGAGAGTTTATTT+AGG - 50482:13888-13907 None:intergenic 20.0%
!! TTATCTCATCAAAAATTAAG+AGG + 50482:14033-14052 MS.gene058544:intron 20.0%
!! TTTATTATGATTATACTGAG+AGG + 50482:14677-14696 MS.gene058544:intron 20.0%
!!! GAGTTAAAAAATTTGTTTGT+TGG + 50482:14140-14159 MS.gene058544:intron 20.0%
! TTCATAGTAGAAAACAACAA+AGG - 50482:14638-14657 None:intergenic 25.0%
!! TATTAAACCTTGTTTGTGAA+CGG - 50482:13849-13868 None:intergenic 25.0%
AAGCCATAAGTATATCTTAC+AGG - 50482:14109-14128 None:intergenic 30.0%
ACAAGAACATGTCTATGAAT+TGG + 50482:14451-14470 MS.gene058544:CDS 30.0%
AGAAAATACATGTGGTTAAG+CGG + 50482:13979-13998 MS.gene058544:intron 30.0%
CAAGAACATGTCTATGAATT+GGG + 50482:14452-14471 MS.gene058544:CDS 30.0%
GCAACACAATATGAATATTC+AGG - 50482:14429-14448 None:intergenic 30.0%
TAATCGACATAGACTTTCAA+GGG - 50482:14706-14725 None:intergenic 30.0%
TCCTGCTTGAATATATTTGT+GGG + 50482:13946-13965 MS.gene058544:intron 30.0%
TTAATCGACATAGACTTTCA+AGG - 50482:14707-14726 None:intergenic 30.0%
!!! TTTTATTTGTTGTCAACCTC+AGG + 50482:14360-14379 MS.gene058544:intron 30.0%
ATCAATGTGTTGTACCTTCT+TGG - 50482:14511-14530 None:intergenic 35.0%
CAATAAGATTATCACGTTCC+TGG - 50482:14821-14840 None:intergenic 35.0%
CCAAACAAACCATTCAGATT+GGG - 50482:14387-14406 None:intergenic 35.0%
CCTGCTTGAATATATTTGTG+GGG + 50482:13947-13966 MS.gene058544:intron 35.0%
CTCCTGCTTGAATATATTTG+TGG + 50482:13945-13964 MS.gene058544:intron 35.0%
GTTTGTTTGGAAGTCAATCT+GGG + 50482:14397-14416 MS.gene058544:CDS 35.0%
TCAATGTGTTGTACCTTCTT+GGG - 50482:14510-14529 None:intergenic 35.0%
TCCAAACAAACCATTCAGAT+TGG - 50482:14388-14407 None:intergenic 35.0%
! AGAAGGTACAACACATTGAT+TGG + 50482:14511-14530 MS.gene058544:intron 35.0%
AAATACATGTGGTTAAGCGG+AGG + 50482:13982-14001 MS.gene058544:intron 40.0%
CAATGTGTTGTACCTTCTTG+GGG - 50482:14509-14528 None:intergenic 40.0%
CCCCACAAATATATTCAAGC+AGG - 50482:13950-13969 None:intergenic 40.0%
GAAATACCATCTTCGTATCG+AGG - 50482:14776-14795 None:intergenic 40.0%
GAACATGTCTATGAATTGGG+AGG + 50482:14455-14474 MS.gene058544:CDS 40.0%
GAATTGGGAGGAAAAGATCA+TGG + 50482:14467-14486 MS.gene058544:CDS 40.0%
GGGCCTGTAAGATATACTTA+TGG + 50482:14103-14122 MS.gene058544:intron 40.0%
! ATATATTCAAGCAGGAGCAC+AGG - 50482:13942-13961 None:intergenic 40.0%
! GGTTTGTTTGGAAGTCAATC+TGG + 50482:14396-14415 MS.gene058544:CDS 40.0%
!! CCCAATCTGAATGGTTTGTT+TGG + 50482:14384-14403 MS.gene058544:CDS 40.0%
AATCCGCACTCCAAAAACAC+GGG + 50482:14082-14101 MS.gene058544:intron 45.0%
ATAGGAGCCGTTCACAAACA+AGG + 50482:13839-13858 MS.gene058544:CDS 45.0%
ATCACTCCTGAACTTCTCAC+CGG - 50482:13798-13817 None:intergenic 45.0%
CAATCCGCACTCCAAAAACA+CGG + 50482:14081-14100 MS.gene058544:intron 45.0%
TTACCTGCTTAACCCCAAGA+AGG + 50482:14494-14513 MS.gene058544:CDS 45.0%
TTTGTGAACGGCTCCTATGT+TGG - 50482:13837-13856 None:intergenic 45.0%
! TTTTCTTCTCGTGCGTTGCA+CGG + 50482:14293-14312 MS.gene058544:intron 45.0%
ACCATTCAGATTGGGTCCTG+AGG - 50482:14379-14398 None:intergenic 50.0%
ACCTCAGGACCCAATCTGAA+TGG + 50482:14375-14394 MS.gene058544:intron 50.0%
ATCCGCACTCCAAAAACACG+GGG + 50482:14083-14102 MS.gene058544:intron 50.0%
ATTATCACGTTCCTGGCCCT+GGG - 50482:14814-14833 None:intergenic 50.0%
GCACATCCTCGATACGAAGA+TGG + 50482:14767-14786 MS.gene058544:intron 50.0%
GCTCAATGCCACACCAACAT+AGG + 50482:13821-13840 MS.gene058544:CDS 50.0%
GTACCTTCTTGGGGTTAAGC+AGG - 50482:14500-14519 None:intergenic 50.0%
TTTCTTCTCGTGCGTTGCAC+GGG + 50482:14294-14313 MS.gene058544:intron 50.0%
! TCTTACAGGCCCCGTGTTTT+TGG - 50482:14095-14114 None:intergenic 50.0%
CATGTGGTTAAGCGGAGGTC+AGG + 50482:13987-14006 MS.gene058544:intron 55.0%
CCTGCTCTTAAGAAGCCCCA+GGG + 50482:14795-14814 MS.gene058544:CDS 55.0%
GAACGGCTCCTATGTTGGTG+TGG - 50482:13832-13851 None:intergenic 55.0%
GATTATCACGTTCCTGGCCC+TGG - 50482:14815-14834 None:intergenic 55.0%
TCCTGCTCTTAAGAAGCCCC+AGG + 50482:14794-14813 MS.gene058544:CDS 55.0%
TGAACTTCTCACCGGCAGCA+CGG - 50482:13790-13809 None:intergenic 55.0%
TTATCACGTTCCTGGCCCTG+GGG - 50482:14813-14832 None:intergenic 55.0%
! GCACGGTTTCTGGGAAGTGT+CGG - 50482:13773-13792 None:intergenic 55.0%
CCCTGGGGCTTCTTAAGAGC+AGG - 50482:14798-14817 None:intergenic 60.0%
CTTCCCAGAAACCGTGCTGC+CGG + 50482:13776-13795 MS.gene058544:CDS 60.0%
GTGCTGCCGGTGAGAAGTTC+AGG + 50482:13789-13808 MS.gene058544:CDS 60.0%
GTGGTTAAGCGGAGGTCAGG+AGG + 50482:13990-14009 MS.gene058544:intron 60.0%
TCACCGGCAGCACGGTTTCT+GGG - 50482:13782-13801 None:intergenic 60.0%
TCTTAAGAAGCCCCAGGGCC+AGG + 50482:14800-14819 MS.gene058544:CDS 60.0%
!!! GGCCCCGTGTTTTTGGAGTG+CGG - 50482:14088-14107 None:intergenic 60.0%
CTCACCGGCAGCACGGTTTC+TGG - 50482:13783-13802 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
50482 gene 13755 14854 13755 ID=MS.gene058544
50482 mRNA 13755 14854 13755 ID=MS.gene058544.t1;Parent=MS.gene058544
50482 exon 13755 13860 13755 ID=MS.gene058544.t1.exon1;Parent=MS.gene058544.t1
50482 CDS 13755 13860 13755 ID=cds.MS.gene058544.t1;Parent=MS.gene058544.t1
50482 exon 14382 14515 14382 ID=MS.gene058544.t1.exon2;Parent=MS.gene058544.t1
50482 CDS 14382 14515 14382 ID=cds.MS.gene058544.t1;Parent=MS.gene058544.t1
50482 exon 14768 14854 14768 ID=MS.gene058544.t1.exon3;Parent=MS.gene058544.t1
50482 CDS 14768 14854 14768 ID=cds.MS.gene058544.t1;Parent=MS.gene058544.t1
Gene Sequence

>MS.gene058544

ATGGAGTTGTTCGAACCGACACTTCCCAGAAACCGTGCTGCCGGTGAGAAGTTCAGGAGTGATTGCGCTCAATGCCACACCAACATAGGAGCCGTTCACAAACAAGGTTTAATATTAGTATTCTTTTCTGCCTAAATAAACTCTCATATTTACGTATCATGTGCTACTTAATTAGTTGCAGTTTCCTGTGCTCCTGCTTGAATATATTTGTGGGGACTTTATAAAGAAAATACATGTGGTTAAGCGGAGGTCAGGAGGATATATCTTTAAGTGAACAATTATCTCATCAAAAATTAAGAGGATTTAGAAACAATTCATACACAACTCAATCCGCACTCCAAAAACACGGGGCCTGTAAGATATACTTATGGCTTGAAAATAAAATGAGTTAAAAAATTTGTTTGTTGGATTAACAAATAATAGTGAATTTTGATAAATACATATATGTTAATCAATATTAAATGTATTTCATATTAGGTATAACAAACTATAAGTATATAAAAATATGTATATTTATAGTTAATTTTCTCATAATTAATTTTCTTCTCGTGCGTTGCACGGGTGTAAATACTAGTATCATAATATTCTAAGGATAAAAATGTTTGTTTTATTTGTTGTCAACCTCAGGACCCAATCTGAATGGTTTGTTTGGAAGTCAATCTGGGACAACTCCTGAATATTCATATTGTGTTGCTAACAAGAACATGTCTATGAATTGGGAGGAAAAGATCATGGATGATTACCTGCTTAACCCCAAGAAGGTACAACACATTGATTGGCATTTCAATGTTTCTTTTTAGATCAATAAAGAATATTTATATCAAATGGTTATTAATAATCGCTTGATGCTTCTTTGTATATAATAATATTATCATTCTAACCTTTGTTGTTTTCTACTATGAATAAGCTCTTGTTCCATTAATTTATTATGATTATACTGAGAGGTAACCCTTGAAAGTCTATGTCGATTAAATGATCACTGATTTGTGTATTATTTTTTCATTCTTATGTAGCACATCCTCGATACGAAGATGGTATTTCCTGCTCTTAAGAAGCCCCAGGGCCAGGAACGTGATAATCTTATTGCATATCTGAAATAG

Protein sequence

>MS.gene058544.t1

MELFEPTLPRNRAAGEKFRSDCAQCHTNIGAVHKQGPNLNGLFGSQSGTTPEYSYCVANKNMSMNWEEKIMDDYLLNPKKHILDTKMVFPALKKPQGQERDNLIAYLK