AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58169

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58169.t1 MTR_8g091980 99.679 312 1 0 11 322 31 342 0.0 633
MS.gene58169.t1 MTR_8g091980 99.597 248 1 0 11 258 31 278 0.0 505
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58169.t1 AT5G64813 76.803 319 59 7 11 322 32 342 1.02e-166 467
MS.gene58169.t1 AT5G64813 76.803 319 59 7 11 322 32 342 1.02e-166 467
MS.gene58169.t1 AT5G64813 76.803 319 59 7 11 322 32 342 1.02e-166 467
MS.gene58169.t1 AT5G09910 69.841 315 79 5 11 322 32 333 7.61e-146 414
MS.gene58169.t1 AT5G09910 69.841 315 79 5 11 322 32 333 7.61e-146 414
MS.gene58169.t1 AT5G09910 64.444 315 79 6 11 322 32 316 1.04e-126 365
MS.gene58169.t1 AT5G09910 64.444 315 79 6 11 322 32 316 1.04e-126 365
MS.gene58169.t1 AT5G09910 64.444 315 79 6 11 322 32 316 1.04e-126 365

Find 77 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGTTCACCTTATCGTTAA+AGG 0.183622 4.2:+10188059 MS.gene58169:CDS
CCTGTTCCATATATTGTTAT+TGG 0.188210 4.2:+10188582 MS.gene58169:CDS
CATTTCCAGCACACTACTTA+TGG 0.225792 4.2:+10188214 MS.gene58169:intron
TTGTTACCAATAACAATATA+TGG 0.272612 4.2:-10188588 None:intergenic
CACACTACTTATGGAAATTC+TGG 0.286680 4.2:+10188223 MS.gene58169:CDS
GTTGAACTGTGGGATGTATC+AGG 0.360390 4.2:+10188292 MS.gene58169:CDS
CCTTTGGGATCTGGCGGCCC+TGG 0.368152 4.2:+10188552 MS.gene58169:CDS
TTGAGGCGCTTACTGCAATA+AGG 0.372575 4.2:-10188752 None:intergenic
GCAGCTGAGATTGCTGCAAC+TGG 0.375965 4.2:+10188516 MS.gene58169:CDS
TTCTCATTCTTGCAGGTGTT+GGG 0.377533 4.2:+10188027 MS.gene58169:intron
GAACCAGACAAAGAAAATCT+AGG 0.383439 4.2:-10190686 None:intergenic
ACGCCAATGGGTCGAGAAGC+AGG 0.392805 4.2:+10188671 MS.gene58169:CDS
GATGATCAGTCTTATAATAC+AGG 0.395791 4.2:+10190466 MS.gene58169:CDS
CTACTGTACAACCAATTGTC+TGG 0.410194 4.2:-10188103 None:intergenic
GCTCGTCCTTCCCAGACAAT+TGG 0.412167 4.2:+10188092 MS.gene58169:CDS
TAGCTTTCAGAGACCGAAAC+TGG 0.435235 4.2:-10190656 None:intergenic
CTCTCTCAGAATCACCTTTG+AGG 0.453797 4.2:-10188261 None:intergenic
CCAATAACAATATATGGAAC+AGG 0.458650 4.2:-10188582 None:intergenic
AAAAGAAGCAAGGTATGATA+AGG 0.458953 4.2:+10190203 MS.gene58169:CDS
ATTCGCTGAGAAGATCTTTG+AGG 0.468161 4.2:-10190400 None:intergenic
GTCTGGGAAGGACGAGCAAT+GGG 0.472190 4.2:-10188086 None:intergenic
AGCTGATATTGTTGCAAAAG+AGG 0.478431 4.2:+10188611 MS.gene58169:CDS
CGAAGACTAGTTTGCAGAAG+TGG 0.478441 4.2:+10188493 MS.gene58169:CDS
CTATCCTCAACAACCAGTTT+CGG 0.481026 4.2:+10190643 MS.gene58169:CDS
AGATTGCGTTGAGCCGGAAG+TGG 0.491900 4.2:-10190602 None:intergenic
GGAGTTAGATTGCGTTGAGC+CGG 0.493866 4.2:-10190608 None:intergenic
AACCCTGCTTCTCGACCCAT+TGG 0.497798 4.2:-10188674 None:intergenic
TTGTTGATGTTGCACGCCAA+TGG 0.504775 4.2:+10188658 MS.gene58169:CDS
AGTCAGTGGAAGCTCCTCGC+TGG 0.509155 4.2:-10188706 None:intergenic
ATGAAATACCTGCACCTACA+TGG 0.516650 4.2:+10190365 MS.gene58169:CDS
TGGGAGAACCTTTAACGATA+AGG 0.517212 4.2:-10188067 None:intergenic
AACGATAAGGTGAACTAAAG+AGG 0.519763 4.2:-10188054 None:intergenic
TTGGGATCTGGCGGCCCTGG+TGG 0.522309 4.2:+10188555 MS.gene58169:CDS
TGTTGATGTTGCACGCCAAT+GGG 0.527951 4.2:+10188659 MS.gene58169:CDS
TGAGGATAGAGTGTGGGTGG+TGG 0.530789 4.2:-10190629 None:intergenic
GAGATTTCTTTGTTGAACTG+TGG 0.535232 4.2:+10188281 MS.gene58169:CDS
TGAGAAGATCTTTGAGGTGA+AGG 0.537945 4.2:-10190394 None:intergenic
AGATCTTTGAGGTGAAGGAA+TGG 0.539829 4.2:-10190389 None:intergenic
TACTGTACAACCAATTGTCT+GGG 0.540418 4.2:-10188102 None:intergenic
AGTGGAAGCTCCTCGCTGGA+TGG 0.542183 4.2:-10188702 None:intergenic
AACCAGACAAAGAAAATCTA+GGG 0.551575 4.2:-10190685 None:intergenic
GGAAGCATGTTATACTTGTA+CGG 0.551656 4.2:-10190578 None:intergenic
AGATTTCTTTGTTGAACTGT+GGG 0.551856 4.2:+10188282 MS.gene58169:CDS
GGAATGGACCATGTAGGTGC+AGG 0.556023 4.2:-10190373 None:intergenic
CAGCTGAGATTGCTGCAACT+GGG 0.556342 4.2:+10188517 MS.gene58169:CDS
TGTCTGGGAAGGACGAGCAA+TGG 0.556939 4.2:-10188087 None:intergenic
TGTTGAGGATAGAGTGTGGG+TGG 0.563767 4.2:-10190632 None:intergenic
TCTGTGAATAAAAGAGAGAT+CGG 0.566338 4.2:-10188336 None:intergenic
GATTGCGTTGAGCCGGAAGT+GGG 0.566553 4.2:-10190601 None:intergenic
GCTGATATTGTTGCAAAAGA+GGG 0.567194 4.2:+10188612 MS.gene58169:CDS
GCAATAAGGCCTCCACCACC+AGG 0.569478 4.2:-10188738 None:intergenic
AGCTCTTCAAGTAGCCTCAA+AGG 0.575296 4.2:+10188247 MS.gene58169:CDS
CGCCAATGGGTCGAGAAGCA+GGG 0.583957 4.2:+10188672 MS.gene58169:CDS
GAGGGTGCGAGAGGAAGCAG+CGG 0.585539 4.2:+10188630 MS.gene58169:CDS
AGAAGCAAGGTATGATAAGG+AGG 0.588578 4.2:+10190206 MS.gene58169:CDS
TTGCGTTGAGCCGGAAGTGG+GGG 0.590536 4.2:-10190599 None:intergenic
TATGGAACAGGAAGACCACC+AGG 0.593874 4.2:-10188570 None:intergenic
CCAGGGCCGCCAGATCCCAA+AGG 0.609580 4.2:-10188552 None:intergenic
GATGTGCATCAGTCAACTGT+AGG 0.611599 4.2:+10187745 MS.gene58169:CDS
GTACAACCAATTGTCTGGGA+AGG 0.616763 4.2:-10188098 None:intergenic
CCAGGAAAACTCTCAGTCAG+TGG 0.617823 4.2:-10188720 None:intergenic
TGGTTGTACAGTAGATGTGA+AGG 0.618127 4.2:+10188112 MS.gene58169:CDS
GAAGACTAGTTTGCAGAAGT+GGG 0.629867 4.2:+10188494 MS.gene58169:CDS
TACAGGCAGCAAAAGAAGCA+AGG 0.630672 4.2:+10190193 MS.gene58169:intron
GAGACCGAAACTGGTTGTTG+AGG 0.633597 4.2:-10190647 None:intergenic
GGTTGTTGAGGATAGAGTGT+GGG 0.640336 4.2:-10190635 None:intergenic
GTTGCAAAAGAGGGTGCGAG+AGG 0.651488 4.2:+10188621 MS.gene58169:CDS
ATTGCGTTGAGCCGGAAGTG+GGG 0.655325 4.2:-10190600 None:intergenic
GTTGACTGATGCACATCGAA+CGG 0.659138 4.2:-10187739 None:intergenic
AGGGTGCGAGAGGAAGCAGC+GGG 0.661507 4.2:+10188631 MS.gene58169:CDS
TCAGCTCCTTTGGGATCTGG+CGG 0.672711 4.2:+10188546 MS.gene58169:CDS
ATGATCAGTCTTATAATACA+GGG 0.679216 4.2:+10190467 MS.gene58169:CDS
TGGTTGTTGAGGATAGAGTG+TGG 0.696987 4.2:-10190636 None:intergenic
GGGTTTGCTTCCATCCAGCG+AGG 0.699492 4.2:+10188692 MS.gene58169:CDS
GGTGAAGGAATGGACCATGT+AGG 0.709587 4.2:-10190379 None:intergenic
TAGATGAAATATCTTCTCAG+CGG 0.726315 4.2:+10190422 MS.gene58169:CDS
ATGGAACAGGAAGACCACCA+GGG 0.755247 4.2:-10188569 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTATTTTCAAAATCAAA+AGG + chr4.2:10189881-10189900 MS.gene58169:intron 10.0%
!! ATAAAATTCTATAGAGAATA+TGG + chr4.2:10189361-10189380 MS.gene58169:intron 15.0%
!! TATTAATAAGGGAAAATATT+TGG + chr4.2:10189551-10189570 MS.gene58169:intron 15.0%
!!! AGTAGTTTTAATTTTTTTGT+CGG + chr4.2:10190165-10190184 MS.gene58169:intron 15.0%
!!! TAGAAGTTGATTTTTTTTAT+CGG + chr4.2:10189857-10189876 MS.gene58169:intron 15.0%
!!! TCTAATCTAGTTTTTTTTAA+GGG - chr4.2:10189841-10189860 None:intergenic 15.0%
!!! TTCTAATCTAGTTTTTTTTA+AGG - chr4.2:10189842-10189861 None:intergenic 15.0%
!!! TTTTATAAAACTGAAACAAT+AGG - chr4.2:10188383-10188402 None:intergenic 15.0%
!! TTAGAAATTAGCATTAACAT+CGG - chr4.2:10188858-10188877 None:intergenic 20.0%
!! TTGTTACCAATAACAATATA+TGG - chr4.2:10188591-10188610 None:intergenic 20.0%
!!! ACAAATTGGTAGTATTAATA+AGG + chr4.2:10189539-10189558 MS.gene58169:intron 20.0%
!!! CAAATTGGTAGTATTAATAA+GGG + chr4.2:10189540-10189559 MS.gene58169:intron 20.0%
!!! CAACAACAATAAATTTTGTA+AGG - chr4.2:10190538-10190557 None:intergenic 20.0%
!!! CTATTTCATCTGATATTTTA+TGG - chr4.2:10189158-10189177 None:intergenic 20.0%
!!! TTATTTTCTTCACTAATGTT+TGG - chr4.2:10187907-10187926 None:intergenic 20.0%
! AACAATACATGTATCTTAAG+GGG + chr4.2:10189580-10189599 MS.gene58169:intron 25.0%
! AATAGAGAGAATCATAAAGT+AGG - chr4.2:10187966-10187985 None:intergenic 25.0%
! AATTCATTATCCATAATGAG+TGG - chr4.2:10189974-10189993 None:intergenic 25.0%
! AGAACAATACATGTATCTTA+AGG + chr4.2:10189578-10189597 MS.gene58169:intron 25.0%
! ATAGAGAGAATCATAAAGTA+GGG - chr4.2:10187965-10187984 None:intergenic 25.0%
! ATTAACATCGGAATTATTCA+TGG - chr4.2:10188846-10188865 None:intergenic 25.0%
! CAAATGTGTCTATAATCAAA+GGG - chr4.2:10189498-10189517 None:intergenic 25.0%
! GAACAATACATGTATCTTAA+GGG + chr4.2:10189579-10189598 MS.gene58169:intron 25.0%
! GATGTTAATGCTAATTTCTA+AGG + chr4.2:10188857-10188876 MS.gene58169:intron 25.0%
! GTATACATGTTTAGATTTGA+AGG + chr4.2:10188404-10188423 MS.gene58169:intron 25.0%
! TCAAATGTGTCTATAATCAA+AGG - chr4.2:10189499-10189518 None:intergenic 25.0%
! TCAAGTATTAACAAGTATAG+TGG + chr4.2:10188884-10188903 MS.gene58169:intron 25.0%
! TTAATACTACCAATTTGTCT+TGG - chr4.2:10189537-10189556 None:intergenic 25.0%
! TTCTATAGAGAATATGGAAT+AGG + chr4.2:10189367-10189386 MS.gene58169:intron 25.0%
! TTGGGATGTAAAAAAAGAAT+CGG + chr4.2:10187872-10187891 MS.gene58169:intron 25.0%
! TTTCAAAATCAAAAGGCTAA+TGG + chr4.2:10189888-10189907 MS.gene58169:intron 25.0%
! TTTCATGTCAAAAAAGCTTT+GGG + chr4.2:10187854-10187873 MS.gene58169:intron 25.0%
!! ATGATCAGTCTTATAATACA+GGG + chr4.2:10190467-10190486 MS.gene58169:CDS 25.0%
!! ATTAAGATGTCTCTGAAAAA+TGG - chr4.2:10187990-10188009 None:intergenic 25.0%
!! CTCTTTTATTCACAGATTAA+TGG + chr4.2:10188343-10188362 MS.gene58169:CDS 25.0%
!! TTTTCATGTCAAAAAAGCTT+TGG + chr4.2:10187853-10187872 MS.gene58169:intron 25.0%
!!! ATCACAGGACTTTTAAAAAT+TGG - chr4.2:10189715-10189734 None:intergenic 25.0%
!!! ATGGAATAGCTTTGTTTTAA+TGG - chr4.2:10189071-10189090 None:intergenic 25.0%
!!! GAAATAGAGCTATTTTAGAA+GGG + chr4.2:10189171-10189190 MS.gene58169:intron 25.0%
!!! TGAAATAGAGCTATTTTAGA+AGG + chr4.2:10189170-10189189 MS.gene58169:intron 25.0%
!!! TGGAATAGCTTTGTTTTAAT+GGG - chr4.2:10189070-10189089 None:intergenic 25.0%
AAAAGAAGCAAGGTATGATA+AGG + chr4.2:10190203-10190222 MS.gene58169:CDS 30.0%
AAACATTGAAGAAACACAGA+TGG - chr4.2:10189744-10189763 None:intergenic 30.0%
AACCAGACAAAGAAAATCTA+GGG - chr4.2:10190688-10190707 None:intergenic 30.0%
ACAAATAGTTAAATGCCAAG+TGG - chr4.2:10189133-10189152 None:intergenic 30.0%
AGAGTCAGATTAGTAAAAAC+AGG - chr4.2:10189101-10189120 None:intergenic 30.0%
AGATTTCTTTGTTGAACTGT+GGG + chr4.2:10188282-10188301 MS.gene58169:CDS 30.0%
AGGTAAGCATTTCACATTAT+AGG + chr4.2:10188132-10188151 MS.gene58169:intron 30.0%
AGTAAAGCAGATCATTTCTA+TGG - chr4.2:10189226-10189245 None:intergenic 30.0%
AGTGACACAAAATTTAGTTG+AGG - chr4.2:10188772-10188791 None:intergenic 30.0%
ATGTTTAGATTTGAAGGAAG+TGG + chr4.2:10188410-10188429 MS.gene58169:intron 30.0%
ATTGTTTGAACTTCATTTGC+AGG + chr4.2:10188435-10188454 MS.gene58169:intron 30.0%
CCAATAACAATATATGGAAC+AGG - chr4.2:10188585-10188604 None:intergenic 30.0%
CCTGTTCCATATATTGTTAT+TGG + chr4.2:10188582-10188601 MS.gene58169:CDS 30.0%
CTACTATAAGAAGAATGAAC+TGG - chr4.2:10190150-10190169 None:intergenic 30.0%
GGTAAGCATTTCACATTATA+GGG + chr4.2:10188133-10188152 MS.gene58169:intron 30.0%
GTAAAGCAGATCATTTCTAT+GGG - chr4.2:10189225-10189244 None:intergenic 30.0%
TAAACGATGAATAGTGTGTT+TGG + chr4.2:10187768-10187787 MS.gene58169:intron 30.0%
TAGAGAGAATCATAAAGTAG+GGG - chr4.2:10187964-10187983 None:intergenic 30.0%
TAGATGAAATATCTTCTCAG+CGG + chr4.2:10190422-10190441 MS.gene58169:CDS 30.0%
TCTGTGAATAAAAGAGAGAT+CGG - chr4.2:10188339-10188358 None:intergenic 30.0%
TCTTCTCCTAATCAACTAAA+AGG - chr4.2:10190335-10190354 None:intergenic 30.0%
TGGGATGTAAAAAAAGAATC+GGG + chr4.2:10187873-10187892 MS.gene58169:intron 30.0%
TTAGTTCACCTTATCGTTAA+AGG + chr4.2:10188059-10188078 MS.gene58169:CDS 30.0%
TTCTTTATAGCCACTCATTA+TGG + chr4.2:10189961-10189980 MS.gene58169:intron 30.0%
! ATCATCTAAAGCATAGAAAG+TGG - chr4.2:10188812-10188831 None:intergenic 30.0%
! CTTAAGGGGTCTTATAAATA+GGG + chr4.2:10189594-10189613 MS.gene58169:intron 30.0%
! GATGATCAGTCTTATAATAC+AGG + chr4.2:10190466-10190485 MS.gene58169:CDS 30.0%
! TCTTAAGGGGTCTTATAAAT+AGG + chr4.2:10189593-10189612 MS.gene58169:intron 30.0%
! TGATATTTTCTCATTCTTGC+AGG + chr4.2:10188020-10188039 MS.gene58169:intron 30.0%
!! TTTGTTCTGTACTTTAAAGC+TGG + chr4.2:10188928-10188947 MS.gene58169:intron 30.0%
AACATCGGAATTATTCATGG+AGG - chr4.2:10188843-10188862 None:intergenic 35.0%
AACGATAAGGTGAACTAAAG+AGG - chr4.2:10188057-10188076 None:intergenic 35.0%
AAGCTACTATTTGTAGCTTG+TGG - chr4.2:10187828-10187847 None:intergenic 35.0%
AATTTCCATAAGTAGTGTGC+TGG - chr4.2:10188222-10188241 None:intergenic 35.0%
ACACTGTTTATTCGATCACA+TGG - chr4.2:10189691-10189710 None:intergenic 35.0%
ACCATTTCAGATACAGTGAT+AGG - chr4.2:10189003-10189022 None:intergenic 35.0%
AGAGAGAATCATAAAGTAGG+GGG - chr4.2:10187963-10187982 None:intergenic 35.0%
AGCTGATATTGTTGCAAAAG+AGG + chr4.2:10188611-10188630 MS.gene58169:CDS 35.0%
CACACTACTTATGGAAATTC+TGG + chr4.2:10188223-10188242 MS.gene58169:CDS 35.0%
CATCCATCAACTAAACTCTA+AGG - chr4.2:10189027-10189046 None:intergenic 35.0%
CCATTTCAGATACAGTGATA+GGG - chr4.2:10189002-10189021 None:intergenic 35.0%
GAACCAGACAAAGAAAATCT+AGG - chr4.2:10190689-10190708 None:intergenic 35.0%
GAGATTTCTTTGTTGAACTG+TGG + chr4.2:10188281-10188300 MS.gene58169:CDS 35.0%
GCTGATATTGTTGCAAAAGA+GGG + chr4.2:10188612-10188631 MS.gene58169:CDS 35.0%
GTACCTTAGAGTTTAGTTGA+TGG + chr4.2:10189021-10189040 MS.gene58169:intron 35.0%
GTCAGATTAGTAAAAACAGG+AGG - chr4.2:10189098-10189117 None:intergenic 35.0%
TACTGTACAACCAATTGTCT+GGG - chr4.2:10188105-10188124 None:intergenic 35.0%
TCCTAATCAACTAAAAGGAG+AGG - chr4.2:10190330-10190349 None:intergenic 35.0%
! CTTTTCAGTCCAAGACAAAT+TGG + chr4.2:10189525-10189544 MS.gene58169:intron 35.0%
! GAATAGTGTGTTTGGAATTG+CGG + chr4.2:10187776-10187795 MS.gene58169:intron 35.0%
! GGAAGCATGTTATACTTGTA+CGG - chr4.2:10190581-10190600 None:intergenic 35.0%
! TACCCTAGATTTTCTTTGTC+TGG + chr4.2:10190683-10190702 MS.gene58169:CDS 35.0%
! TACTATTTGTAGCTTGTGGT+TGG - chr4.2:10187824-10187843 None:intergenic 35.0%
! TGTGATTAAGTTTTTCCGCA+TGG + chr4.2:10190230-10190249 MS.gene58169:CDS 35.0%
!! ACCTCTCCTTTTAGTTGATT+AGG + chr4.2:10190326-10190345 MS.gene58169:intron 35.0%
!! AGTTTAGTTGATGGATGAAG+AGG + chr4.2:10189030-10189049 MS.gene58169:intron 35.0%
!!! TTTTTTTGTCGGATGACTAC+AGG + chr4.2:10190176-10190195 MS.gene58169:intron 35.0%
AGAAGCAAGGTATGATAAGG+AGG + chr4.2:10190206-10190225 MS.gene58169:CDS 40.0%
AGTAAAAACAGGAGGCAACA+TGG - chr4.2:10189090-10189109 None:intergenic 40.0%
ATGAAATACCTGCACCTACA+TGG + chr4.2:10190365-10190384 MS.gene58169:CDS 40.0%
ATGGAATAGGTTACTCTGTC+AGG + chr4.2:10189380-10189399 MS.gene58169:intron 40.0%
ATTCGCTGAGAAGATCTTTG+AGG - chr4.2:10190403-10190422 None:intergenic 40.0%
CAAAGACATTACTGACCATG+CGG - chr4.2:10190248-10190267 None:intergenic 40.0%
CACAGATGGTTGAAAATCAC+AGG - chr4.2:10189730-10189749 None:intergenic 40.0%
CATTTCCAGCACACTACTTA+TGG + chr4.2:10188214-10188233 MS.gene58169:intron 40.0%
CCCTATCACTGTATCTGAAA+TGG + chr4.2:10188999-10189018 MS.gene58169:intron 40.0%
CTACTGTACAACCAATTGTC+TGG - chr4.2:10188106-10188125 None:intergenic 40.0%
CTATCCTCAACAACCAGTTT+CGG + chr4.2:10190643-10190662 MS.gene58169:CDS 40.0%
GACTCTCTACTATATCCACT+TGG + chr4.2:10189115-10189134 MS.gene58169:intron 40.0%
GAGAGAATCATAAAGTAGGG+GGG - chr4.2:10187962-10187981 None:intergenic 40.0%
GTAGTGTGCTGGAAATGAAA+CGG - chr4.2:10188211-10188230 None:intergenic 40.0%
TCTGCTTTACTTGACTGATG+AGG + chr4.2:10189235-10189254 MS.gene58169:intron 40.0%
TGGGAGAACCTTTAACGATA+AGG - chr4.2:10188070-10188089 None:intergenic 40.0%
TGGTTGTACAGTAGATGTGA+AGG + chr4.2:10188112-10188131 MS.gene58169:CDS 40.0%
TTCTCATTCTTGCAGGTGTT+GGG + chr4.2:10188027-10188046 MS.gene58169:intron 40.0%
TTTCTCATTCTTGCAGGTGT+TGG + chr4.2:10188026-10188045 MS.gene58169:intron 40.0%
! AGGGGTCTTATAAATAGGGA+CGG + chr4.2:10189598-10189617 MS.gene58169:intron 40.0%
!! AACATGAGAGAAGTGCCTAA+GGG - chr4.2:10189420-10189439 None:intergenic 40.0%
!! AGATCTTTGAGGTGAAGGAA+TGG - chr4.2:10190392-10190411 None:intergenic 40.0%
!! GAAGACTAGTTTGCAGAAGT+GGG + chr4.2:10188494-10188513 MS.gene58169:CDS 40.0%
!! TAACATGAGAGAAGTGCCTA+AGG - chr4.2:10189421-10189440 None:intergenic 40.0%
!! TGAGAAGATCTTTGAGGTGA+AGG - chr4.2:10190397-10190416 None:intergenic 40.0%
!! TTCTGTACTTTAAAGCTGGC+TGG + chr4.2:10188932-10188951 MS.gene58169:intron 40.0%
AGAATCATAAAGTAGGGGGG+AGG - chr4.2:10187959-10187978 None:intergenic 45.0%
AGCTCTTCAAGTAGCCTCAA+AGG + chr4.2:10188247-10188266 MS.gene58169:CDS 45.0%
CTCTCTCAGAATCACCTTTG+AGG - chr4.2:10188264-10188283 None:intergenic 45.0%
GAATCATAAAGTAGGGGGGA+GGG - chr4.2:10187958-10187977 None:intergenic 45.0%
GATGTGCATCAGTCAACTGT+AGG + chr4.2:10187745-10187764 MS.gene58169:CDS 45.0%
GGTTGTTGAGGATAGAGTGT+GGG - chr4.2:10190638-10190657 None:intergenic 45.0%
GTACAACCAATTGTCTGGGA+AGG - chr4.2:10188101-10188120 None:intergenic 45.0%
GTCCTATGTAGAAGACCCTT+AGG + chr4.2:10189402-10189421 MS.gene58169:intron 45.0%
GTTGAACTGTGGGATGTATC+AGG + chr4.2:10188292-10188311 MS.gene58169:CDS 45.0%
GTTGACTGATGCACATCGAA+CGG - chr4.2:10187742-10187761 None:intergenic 45.0%
TACAGGCAGCAAAAGAAGCA+AGG + chr4.2:10190193-10190212 MS.gene58169:intron 45.0%
TAGCTTTCAGAGACCGAAAC+TGG - chr4.2:10190659-10190678 None:intergenic 45.0%
TGCCTAAGGGTCTTCTACAT+AGG - chr4.2:10189407-10189426 None:intergenic 45.0%
TGGTTGTTGAGGATAGAGTG+TGG - chr4.2:10190639-10190658 None:intergenic 45.0%
TGTTGATGTTGCACGCCAAT+GGG + chr4.2:10188659-10188678 MS.gene58169:CDS 45.0%
TTGTTGATGTTGCACGCCAA+TGG + chr4.2:10188658-10188677 MS.gene58169:CDS 45.0%
! GGGACATTTTCAGCTCCTTT+GGG + chr4.2:10188537-10188556 MS.gene58169:CDS 45.0%
! TGGGACATTTTCAGCTCCTT+TGG + chr4.2:10188536-10188555 MS.gene58169:CDS 45.0%
! TTGAGGCGCTTACTGCAATA+AGG - chr4.2:10188755-10188774 None:intergenic 45.0%
!! CGAAGACTAGTTTGCAGAAG+TGG + chr4.2:10188493-10188512 MS.gene58169:CDS 45.0%
!!! TTTTCAGCTCCTTTGGGATC+TGG + chr4.2:10188543-10188562 MS.gene58169:CDS 45.0%
!! TAAATAAATAAATAAATAAG+AGG - chr4.2:10190096-10190115 None:intergenic 5.0%
!! TAAATTTAAACAAAAAATAA+AGG - chr4.2:10190281-10190300 None:intergenic 5.0%
!!! TATTTTTTGTTTAAATTTAT+GGG + chr4.2:10190282-10190301 MS.gene58169:intron 5.0%
!!! TTATTTTTTGTTTAAATTTA+TGG + chr4.2:10190281-10190300 MS.gene58169:intron 5.0%
ATGGAACAGGAAGACCACCA+GGG - chr4.2:10188572-10188591 None:intergenic 50.0%
CAGCTGAGATTGCTGCAACT+GGG + chr4.2:10188517-10188536 MS.gene58169:CDS 50.0%
CCAGGAAAACTCTCAGTCAG+TGG - chr4.2:10188723-10188742 None:intergenic 50.0%
GAGACCGAAACTGGTTGTTG+AGG - chr4.2:10190650-10190669 None:intergenic 50.0%
GGAGTTAGATTGCGTTGAGC+CGG - chr4.2:10190611-10190630 None:intergenic 50.0%
GGTGAAGGAATGGACCATGT+AGG - chr4.2:10190382-10190401 None:intergenic 50.0%
GTGTGCTGGAAATGAAACGG+AGG - chr4.2:10188208-10188227 None:intergenic 50.0%
TAACATGCTTCCCCCACTTC+CGG + chr4.2:10190589-10190608 MS.gene58169:CDS 50.0%
TATGGAACAGGAAGACCACC+AGG - chr4.2:10188573-10188592 None:intergenic 50.0%
TGTTGAGGATAGAGTGTGGG+TGG - chr4.2:10190635-10190654 None:intergenic 50.0%
! ACTGAGAGTTTTCCTGGTGG+TGG + chr4.2:10188726-10188745 MS.gene58169:CDS 50.0%
! CTGACTGAGAGTTTTCCTGG+TGG + chr4.2:10188723-10188742 MS.gene58169:CDS 50.0%
!! CCACTGACTGAGAGTTTTCC+TGG + chr4.2:10188720-10188739 MS.gene58169:CDS 50.0%
AACCCTGCTTCTCGACCCAT+TGG - chr4.2:10188677-10188696 None:intergenic 55.0%
AGATTGCGTTGAGCCGGAAG+TGG - chr4.2:10190605-10190624 None:intergenic 55.0%
AGGGCAAGCAGTCACATGCT+CGG - chr4.2:10187939-10187958 None:intergenic 55.0%
ATTGCGTTGAGCCGGAAGTG+GGG - chr4.2:10190603-10190622 None:intergenic 55.0%
GATTGCGTTGAGCCGGAAGT+GGG - chr4.2:10190604-10190623 None:intergenic 55.0%
GCAGCTGAGATTGCTGCAAC+TGG + chr4.2:10188516-10188535 MS.gene58169:CDS 55.0%
GCTCGTCCTTCCCAGACAAT+TGG + chr4.2:10188092-10188111 MS.gene58169:CDS 55.0%
GGAATGGACCATGTAGGTGC+AGG - chr4.2:10190376-10190395 None:intergenic 55.0%
GTCTGGGAAGGACGAGCAAT+GGG - chr4.2:10188089-10188108 None:intergenic 55.0%
TGAGGATAGAGTGTGGGTGG+TGG - chr4.2:10190632-10190651 None:intergenic 55.0%
TGTCTGGGAAGGACGAGCAA+TGG - chr4.2:10188090-10188109 None:intergenic 55.0%
! GTTGCAAAAGAGGGTGCGAG+AGG + chr4.2:10188621-10188640 MS.gene58169:CDS 55.0%
!! TCAGCTCCTTTGGGATCTGG+CGG + chr4.2:10188546-10188565 MS.gene58169:CDS 55.0%
!!! GAGAGTTTTCCTGGTGGTGG+AGG + chr4.2:10188729-10188748 MS.gene58169:CDS 55.0%
ACGCCAATGGGTCGAGAAGC+AGG + chr4.2:10188671-10188690 MS.gene58169:CDS 60.0%
AGTCAGTGGAAGCTCCTCGC+TGG - chr4.2:10188709-10188728 None:intergenic 60.0%
AGTGGAAGCTCCTCGCTGGA+TGG - chr4.2:10188705-10188724 None:intergenic 60.0%
CGCCAATGGGTCGAGAAGCA+GGG + chr4.2:10188672-10188691 MS.gene58169:CDS 60.0%
GCAATAAGGCCTCCACCACC+AGG - chr4.2:10188741-10188760 None:intergenic 60.0%
TTGCGTTGAGCCGGAAGTGG+GGG - chr4.2:10190602-10190621 None:intergenic 60.0%
! GGGTTTGCTTCCATCCAGCG+AGG + chr4.2:10188692-10188711 MS.gene58169:CDS 60.0%
AGGGTGCGAGAGGAAGCAGC+GGG + chr4.2:10188631-10188650 MS.gene58169:CDS 65.0%
! GAGGGTGCGAGAGGAAGCAG+CGG + chr4.2:10188630-10188649 MS.gene58169:CDS 65.0%
CCAGGGCCGCCAGATCCCAA+AGG - chr4.2:10188555-10188574 None:intergenic 70.0%
TTGGGATCTGGCGGCCCTGG+TGG + chr4.2:10188555-10188574 MS.gene58169:CDS 70.0%
!! CCTTTGGGATCTGGCGGCCC+TGG + chr4.2:10188552-10188571 MS.gene58169:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 10187736 10190760 10187736 ID=MS.gene58169
chr4.2 mRNA 10187736 10190760 10187736 ID=MS.gene58169.t1;Parent=MS.gene58169
chr4.2 exon 10187736 10187766 10187736 ID=MS.gene58169.t1.exon1;Parent=MS.gene58169.t1
chr4.2 CDS 10187736 10187766 10187736 ID=cds.MS.gene58169.t1;Parent=MS.gene58169.t1
chr4.2 exon 10188042 10188133 10188042 ID=MS.gene58169.t1.exon2;Parent=MS.gene58169.t1
chr4.2 CDS 10188042 10188133 10188042 ID=cds.MS.gene58169.t1;Parent=MS.gene58169.t1
chr4.2 exon 10188223 10188364 10188223 ID=MS.gene58169.t1.exon3;Parent=MS.gene58169.t1
chr4.2 CDS 10188223 10188364 10188223 ID=cds.MS.gene58169.t1;Parent=MS.gene58169.t1
chr4.2 exon 10188457 10188761 10188457 ID=MS.gene58169.t1.exon4;Parent=MS.gene58169.t1
chr4.2 CDS 10188457 10188761 10188457 ID=cds.MS.gene58169.t1;Parent=MS.gene58169.t1
chr4.2 exon 10190198 10190251 10190198 ID=MS.gene58169.t1.exon5;Parent=MS.gene58169.t1
chr4.2 CDS 10190198 10190251 10190198 ID=cds.MS.gene58169.t1;Parent=MS.gene58169.t1
chr4.2 exon 10190340 10190488 10190340 ID=MS.gene58169.t1.exon6;Parent=MS.gene58169.t1
chr4.2 CDS 10190340 10190488 10190340 ID=cds.MS.gene58169.t1;Parent=MS.gene58169.t1
chr4.2 exon 10190565 10190760 10190565 ID=MS.gene58169.t1.exon7;Parent=MS.gene58169.t1
chr4.2 CDS 10190565 10190760 10190565 ID=cds.MS.gene58169.t1;Parent=MS.gene58169.t1
Gene Sequence

>MS.gene58169

ATGCCGTTCGATGTGCATCAGTCAACTGTAGGTAAACGATGAATAGTGTGTTTGGAATTGCGGTCAAATTGCTTCAGACGCGCATCCAACCACAAGCTACAAATAGTAGCTTCACTATTTTCATGTCAAAAAAGCTTTGGGATGTAAAAAAAGAATCGGGACGTGAATCCAAACATTAGTGAAGAAAATAATTTTATTTTCCGAGCATGTGACTGCTTGCCCTCCCCCCTACTTTATGATTCTCTCTATTACCATTTTTCAGAGACATCTTAATTGCAGATATATGATATTTTCTCATTCTTGCAGGTGTTGGGAAGTCCTCTTTAGTTCACCTTATCGTTAAAGGTTCTCCCATTGCTCGTCCTTCCCAGACAATTGGTTGTACAGTAGATGTGAAGGTAAGCATTTCACATTATAGGGTTACACAAGAGAATACATTGTTTCTGTAATTCAAAGTTTTACTGAACATCCTCCGTTTCATTTCCAGCACACTACTTATGGAAATTCTGGCAGCTCTTCAAGTAGCCTCAAAGGTGATTCTGAGAGAGATTTCTTTGTTGAACTGTGGGATGTATCAGGACATGAACGTTATAAAGATTGCCGATCTCTCTTTTATTCACAGATTAATGGTGAGCAACAAAGTTCCTATTGTTTCAGTTTTATAAAATGTATACATGTTTAGATTTGAAGGAAGTGGTTATTGTTTGAACTTCATTTGCAGGTGTAATTTTTGTTCACGATCTTTCACAGAGAAGAACGAAGACTAGTTTGCAGAAGTGGGCAGCTGAGATTGCTGCAACTGGGACATTTTCAGCTCCTTTGGGATCTGGCGGCCCTGGTGGTCTTCCTGTTCCATATATTGTTATTGGTAACAAAGCTGATATTGTTGCAAAAGAGGGTGCGAGAGGAAGCAGCGGGAATCTTGTTGATGTTGCACGCCAATGGGTCGAGAAGCAGGGTTTGCTTCCATCCAGCGAGGAGCTTCCACTGACTGAGAGTTTTCCTGGTGGTGGAGGCCTTATTGCAGTAAGCGCCTCAACTAAATTTTGTGTCACTTTTACAAGTTTGAGATGCCACTTTCTATGCTTTAGATGATATATGCTACCTCCATGAATAATTCCGATGTTAATGCTAATTTCTAAGGAGTTTCAAGTATTAACAAGTATAGTGGCAGTTCAGAATTTTAGTTGTGTTTGTTCTGTACTTTAAAGCTGGCTGGAATTTTACAAATACATATTAAACGAGTTTTACAATTTTTTTTTACCCTATCACTGTATCTGAAATGGTACCTTAGAGTTTAGTTGATGGATGAAGAGGAATTTTATGCAATGCCCATTAAAACAAAGCTATTCCATGTTGCCTCCTGTTTTTACTAATCTGACTCTCTACTATATCCACTTGGCATTTAACTATTTGTATCCATAAAATATCAGATGAAATAGAGCTATTTTAGAAGGGATATAATGAAATCTTTTGATCAGTTGTTCCCATAGAAATGATCTGCTTTACTTGACTGATGAGGACAACGAATAGTACATTAACAAAAAAAAATCTAAATAGAAATAAGAAAAAAAAAAATCTAAATAGAAGTAAATATTGATAGTTTCTCTCTATACTCTATTGAGATAAAATTCTATAGAGAATATGGAATAGGTTACTCTGTCAGGTCCTATGTAGAAGACCCTTAGGCACTTCTCTCATGTTAAGAAAATTTGTTGTGTAATGAATGTATATGCTGTGTGTGTCTATTACTAAATTACCCTTTGATTATAGACACATTTGATTTTGACTTTTCAGTCCAAGACAAATTGGTAGTATTAATAAGGGAAAATATTTGGAAAAAGAACAATACATGTATCTTAAGGGGTCTTATAAATAGGGACGGATGAAGTATAGAATAAAATAAAATAGAAAAAAGACAATGATGATAAATAGTGTTCTCATTCAAAATACCATGTGATCGAATAAACAGTGTTCCAATTTTTAAAAGTCCTGTGATTTTCAACCATCTGTGTTTCTTCAATGTTTGAATATTATGAAATACTTGCATTGAAACTTCACACTTCACTTTGTTGCTAACTATCTAGATTAGAAGTAGAAGTCCCTTAAAAAAAACTAGATTAGAAGTTGATTTTTTTTATCGGTAATTTATTTTCAAAATCAAAAGGCTAATGGTTAAAATTGTATTTCTAACAGTAGTTTCTTGAAGCATGTCAAATCATATTTTCTTTATAGCCACTCATTATGGATAATGAATTTAGCTCAATCACTGCTACATTGCACACTGTGATGTATTCAGAACAGTTGTGCTTATGAAGTAACAAATCATATCTGATAGAATTTGATGCATAAATCTGCCTCTTATTTATTTATTTATTTATTTATTTATTTATTCATTTAATAGTTGCTATCCAGTTCATTCTTCTTATAGTAGTTTTAATTTTTTTGTCGGATGACTACAGGCAGCAAAAGAAGCAAGGTATGATAAGGAGGCTGTGATTAAGTTTTTCCGCATGGTCAGTAATGTCTTTGATAACTTTATCCTTTATTTTTTGTTTAAATTTATGGGTTGTCATCTAAATTTTCTTGTACCTCTCCTTTTAGTTGATTAGGAGAAGATATTTCTCAGATGAAATACCTGCACCTACATGGTCCATTCCTTCACCTCAAAGATCTTCTCAGCGAATAGATGAAATATCTTCTCAGCGGATAGATGAAAGTTTTATTGATGATGATCAGTCTTATAATACAGGGTATTTTCTGCTTCTTGTCTAAATCATTTTCATGTTTTGTGCAACTCCTTACAAAATTTATTGTTGTTGATTGCAGTCGAAGCAGTGATCCGTACAAGTATAACATGCTTCCCCCACTTCCGGCTCAACGCAATCTAACTCCACCACCCACACTCTATCCTCAACAACCAGTTTCGGTCTCTGAAAGCTACAGTTACCCTAGATTTTCTTTGTCTGGTTCGTCAGAAATGAGTGCTTCATCGAGAACAAAGCGCTCAGATATTAATGTTTGA
Protein sequence

>MS.gene58169.t1

MPFDVHQSTVGVGKSSLVHLIVKGSPIARPSQTIGCTVDVKHTTYGNSGSSSSSLKGDSERDFFVELWDVSGHERYKDCRSLFYSQINGVIFVHDLSQRRTKTSLQKWAAEIAATGTFSAPLGSGGPGGLPVPYIVIGNKADIVAKEGARGSSGNLVDVARQWVEKQGLLPSSEELPLTESFPGGGGLIAAAKEARYDKEAVIKFFRMLIRRRYFSDEIPAPTWSIPSPQRSSQRIDEISSQRIDESFIDDDQSYNTGRSSDPYKYNMLPPLPAQRNLTPPPTLYPQQPVSVSESYSYPRFSLSGSSEMSASSRTKRSDINV