AlfalfaGEDB | MS.gene58518

M. sativa cultivar XinJiangDaYe / MS.gene58518

KEGG: K03671
GO: GO:0003674 GO:0003824 GO:0006662 GO:0008150 GO:0008152 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0018904 GO:0019725 GO:0042592 GO:0044281 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008 GO:0071704
PFAM: PF00085.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58518.t1	XP_003613544.1	96	126	5	0	1	126	1	126	5.90E-63	250

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58518.t1	Q38879	47.7	111	58	0	15	125	22	132	1.6e-29	130.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58518.t1	D8L5E0	96.0	126	5	0	1	126	1	126	4.3e-63	250.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050971	MS.gene58518	0.810308	1.23E-50	-1.69E-46
MS.gene05316	MS.gene58518	0.811128	8.17E-51	-1.69E-46
MS.gene057609	MS.gene58518	0.932261	1.11E-94	-1.69E-46
MS.gene057610	MS.gene58518	0.942162	1.17E-101	-1.69E-46
MS.gene058651	MS.gene58518	0.804244	2.37E-49	-1.69E-46
MS.gene058949	MS.gene58518	0.805184	1.51E-49	-1.69E-46
MS.gene059876	MS.gene58518	0.81558	8.59E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58518.t1	MTR_5g037890	96.032	126	5	0	1	126	1	126	1.60e-90	258
MS.gene58518.t1	MTR_5g037950	67.460	126	40	1	1	125	1	126	1.15e-52	162
MS.gene58518.t1	MTR_5g037930	65.625	128	42	1	1	126	1	128	2.01e-51	159
MS.gene58518.t1	MTR_7g009070	54.717	106	48	0	20	125	28	133	1.15e-42	137
MS.gene58518.t1	MTR_3g112410	46.847	111	59	0	14	124	2	112	4.91e-36	120
MS.gene58518.t1	MTR_1g023140	40.708	113	67	0	14	126	2	114	3.98e-33	112
MS.gene58518.t1	MTR_5g021180	42.342	111	64	0	14	124	4	114	6.22e-33	112
MS.gene58518.t1	MTR_8g107460	41.346	104	61	0	18	121	254	357	3.93e-24	95.1
MS.gene58518.t1	MTR_7g073680	34.286	105	69	0	17	121	23	127	3.76e-22	85.5
MS.gene58518.t1	MTR_8g005320	32.381	105	71	0	17	121	25	129	4.10e-22	85.1
MS.gene58518.t1	MTR_2g010750	37.143	105	66	0	18	122	9	113	9.89e-22	84.0
MS.gene58518.t1	MTR_8g006685	32.381	105	71	0	17	121	94	198	7.01e-21	84.0
MS.gene58518.t1	MTR_8g006560	32.381	105	71	0	17	121	94	198	7.01e-21	84.0
MS.gene58518.t1	MTR_4g081380	35.514	107	65	2	18	122	10	114	8.11e-20	79.0
MS.gene58518.t1	MTR_2g082590	30.476	105	73	0	18	122	10	114	2.07e-18	75.1
MS.gene58518.t1	MTR_2g082590	31.858	113	68	2	10	122	24	127	3.94e-18	75.1
MS.gene58518.t1	MTR_8g059015	35.227	88	55	2	38	123	87	174	2.15e-14	66.2
MS.gene58518.t1	MTR_1g098660	32.222	90	59	2	39	126	77	166	2.26e-14	65.9
MS.gene58518.t1	MTR_3g089065	32.323	99	62	3	27	123	76	171	4.62e-14	65.5
MS.gene58518.t1	MTR_7g080250	36.364	88	55	1	40	126	94	181	5.94e-14	65.1
MS.gene58518.t1	MTR_4g094690	32.927	82	53	1	44	123	85	166	6.92e-14	64.7
MS.gene58518.t1	MTR_2g079420	35.227	88	55	2	38	123	84	171	1.57e-13	63.9
MS.gene58518.t1	MTR_1g114290	31.646	79	50	2	38	115	84	159	2.54e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58518.t1	AT5G39950	47.748	111	58	0	15	125	22	132	2.19e-41	134
MS.gene58518.t1	AT1G69880	46.729	107	57	0	17	123	36	142	9.17e-35	117
MS.gene58518.t1	AT1G59730	39.844	128	74	1	1	125	1	128	4.21e-34	115
MS.gene58518.t1	AT3G51030	40.909	110	65	0	14	123	2	111	4.22e-32	110
MS.gene58518.t1	AT1G19730	44.737	114	62	1	14	126	2	115	1.91e-31	108
MS.gene58518.t1	AT1G45145	42.056	107	62	0	18	124	5	111	4.02e-30	105
MS.gene58518.t1	AT5G42980	41.818	110	64	0	17	126	4	113	1.06e-29	103
MS.gene58518.t1	AT3G17880	39.806	103	62	0	19	121	264	366	4.99e-22	89.7
MS.gene58518.t1	AT3G17880	39.048	105	64	0	20	124	272	376	4.75e-21	87.0
MS.gene58518.t1	AT3G56420	35.870	92	59	0	28	119	50	141	3.69e-20	80.9
MS.gene58518.t1	AT3G56420	35.870	92	59	0	28	119	50	141	3.69e-20	80.9
MS.gene58518.t1	AT3G56420	35.870	92	59	0	28	119	50	141	3.69e-20	80.9
MS.gene58518.t1	AT3G08710	31.532	111	74	1	11	121	18	126	2.35e-19	78.2
MS.gene58518.t1	AT3G08710	31.532	111	74	1	11	121	18	126	2.35e-19	78.2
MS.gene58518.t1	AT3G08710	31.532	111	74	1	11	121	18	126	2.35e-19	78.2
MS.gene58518.t1	AT1G11530	32.381	105	71	0	18	122	2	106	9.52e-18	73.6
MS.gene58518.t1	AT2G40790	33.696	92	61	0	28	119	50	141	4.07e-16	70.1
MS.gene58518.t1	AT2G40790	33.696	92	61	0	28	119	50	141	4.07e-16	70.1
MS.gene58518.t1	AT1G43560	28.906	128	85	4	1	122	33	160	1.78e-15	68.9
MS.gene58518.t1	AT5G16400	37.500	88	54	1	40	126	97	184	2.62e-15	68.9
MS.gene58518.t1	AT4G03520	38.554	83	49	2	43	123	101	183	4.52e-15	68.2
MS.gene58518.t1	AT3G02730	39.080	87	52	1	41	126	88	174	4.99e-15	68.2
MS.gene58518.t1	AT1G03680	38.272	81	48	2	45	123	97	177	1.14e-14	67.0
MS.gene58518.t1	AT1G31020	31.667	120	76	2	6	123	41	156	1.32e-14	66.6
MS.gene58518.t1	AT1G76760	28.421	95	61	3	33	120	70	164	1.27e-13	64.3
MS.gene58518.t1	AT2G35010	37.500	80	46	2	47	123	113	191	3.77e-13	63.2
MS.gene58518.t1	AT2G35010	37.500	80	46	2	47	123	113	191	3.77e-13	63.2
MS.gene58518.t1	AT1G50320	35.443	79	47	2	38	115	85	160	1.55e-11	58.9
MS.gene58518.t1	AT1G52990	31.868	91	60	2	34	123	222	311	1.99e-11	60.1

Find 21 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTCCATTCCACTGCTAAA+TGG	0.238150	5.3:-26038137	MS.gene58518:CDS
GCTTGATGTTGATGAGTTAT+TGG	0.264813	5.3:-26037885	MS.gene58518:intron
TTATTGATTTCACAGCTAAA+TGG	0.322109	5.3:-26037982	MS.gene58518:CDS
TTTCAAAGAAACTAATAAAC+TGG	0.393712	5.3:-26038097	MS.gene58518:intron
TTCTTAATGAGTAGAAAAGT+TGG	0.438267	5.3:+26037634	None:intergenic
AGGAAAAGTTGTTGATAAAG+TGG	0.478902	5.3:-26037611	MS.gene58518:CDS
TAAAGTGGTGGGAGTAAGAA+AGG	0.479129	5.3:-26037596	MS.gene58518:CDS
GGGAGTTTCTCAAACATTCC+AGG	0.482800	5.3:-26037668	MS.gene58518:CDS
GTGTGGACCTTGCAAAGTTA+TGG	0.487071	5.3:-26037960	MS.gene58518:CDS
TAGCAGTGGAATGGAATGTA+AGG	0.498154	5.3:+26038142	None:intergenic
ATACACAAAATTATACAAGT+TGG	0.527315	5.3:+26038194	None:intergenic
CATTCCATTTAGCAGTGGAA+TGG	0.530623	5.3:+26038133	None:intergenic
TGGAATGGAATGTAAGGACA+TGG	0.568326	5.3:+26038148	None:intergenic
AAAAGTTGTTGATAAAGTGG+TGG	0.577415	5.3:-26037608	MS.gene58518:CDS
AAGTTGGCAATGAATGCACC+TGG	0.579245	5.3:+26037650	None:intergenic
GATTTCACAGCTAAATGGTG+TGG	0.590907	5.3:-26037977	MS.gene58518:CDS
AAAGTTGTTGATAAAGTGGT+GGG	0.597105	5.3:-26037607	MS.gene58518:CDS
ACTGGATCCATAACTTTGCA+AGG	0.602265	5.3:+26037953	None:intergenic
ATGAGCATTCCATTTAGCAG+TGG	0.609708	5.3:+26038128	None:intergenic
TAAGAAAGGAGCAACTACAA+AGG	0.660815	5.3:-26037582	MS.gene58518:CDS
GGAATGGAATGTAAGGACAT+GGG	0.736550	5.3:+26038149	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATAATAGCATTTATAAT+AGG	+	chr5.3:26038009-26038028	None:intergenic	10.0%
!!	ATACACAAAATTATACAAGT+TGG	+	chr5.3:26037568-26037587	None:intergenic	20.0%
!!	TTTCAAAGAAACTAATAAAC+TGG	-	chr5.3:26037662-26037681	MS.gene58518:CDS	20.0%
!!!	TCTTATGTAGTATTTTTCTT+TGG	-	chr5.3:26037745-26037764	MS.gene58518:intron	20.0%
!!!	TGTTTTTTCCACAATATAAA+TGG	-	chr5.3:26038063-26038082	MS.gene58518:intron	20.0%
!	TAACAAACTAGTTAAAGTGA+AGG	+	chr5.3:26037915-26037934	None:intergenic	25.0%
!	TTCTTAATGAGTAGAAAAGT+TGG	+	chr5.3:26038128-26038147	None:intergenic	25.0%
!!	ACTTTTCTACTCATTAAGAA+AGG	-	chr5.3:26038128-26038147	MS.gene58518:CDS	25.0%
!!	GTATTTTTCTTTGGTATAGA+TGG	-	chr5.3:26037754-26037773	MS.gene58518:intron	25.0%
!!!	TTATTGATTTCACAGCTAAA+TGG	-	chr5.3:26037777-26037796	MS.gene58518:intron	25.0%
	AATTAAACCTGGCATAAAGT+TGG	+	chr5.3:26037949-26037968	None:intergenic	30.0%
!	AAAAGTTGTTGATAAAGTGG+TGG	-	chr5.3:26038151-26038170	MS.gene58518:CDS	30.0%
!	AAAGTTGTTGATAAAGTGGT+GGG	-	chr5.3:26038152-26038171	MS.gene58518:CDS	30.0%
!	AGGAAAAGTTGTTGATAAAG+TGG	-	chr5.3:26038148-26038167	MS.gene58518:CDS	30.0%
!	GCAGCAAAATCTTTTATAAC+TGG	+	chr5.3:26037827-26037846	None:intergenic	30.0%
	ACCAACTGAGTAATTAAACC+TGG	+	chr5.3:26037960-26037979	None:intergenic	35.0%
	CCACAATATAAATGGCAACA+GGG	-	chr5.3:26038071-26038090	MS.gene58518:intron	35.0%
	TAAGAAAGGAGCAACTACAA+AGG	-	chr5.3:26038177-26038196	MS.gene58518:CDS	35.0%
	TCCACAATATAAATGGCAAC+AGG	-	chr5.3:26038070-26038089	MS.gene58518:intron	35.0%
	TGTAAAACCAACTTTATGCC+AGG	-	chr5.3:26037939-26037958	MS.gene58518:CDS	35.0%
!	GCTTGATGTTGATGAGTTAT+TGG	-	chr5.3:26037874-26037893	MS.gene58518:intron	35.0%
	ACTGGATCCATAACTTTGCA+AGG	+	chr5.3:26037809-26037828	None:intergenic	40.0%
	ATGAGCATTCCATTTAGCAG+TGG	+	chr5.3:26037634-26037653	None:intergenic	40.0%
	CATTCCATTCCACTGCTAAA+TGG	-	chr5.3:26037622-26037641	MS.gene58518:CDS	40.0%
	CATTCCATTTAGCAGTGGAA+TGG	+	chr5.3:26037629-26037648	None:intergenic	40.0%
	GATTTCACAGCTAAATGGTG+TGG	-	chr5.3:26037782-26037801	MS.gene58518:intron	40.0%
	GCCAGGTTTAATTACTCAGT+TGG	-	chr5.3:26037956-26037975	MS.gene58518:CDS	40.0%
	GGAATGGAATGTAAGGACAT+GGG	+	chr5.3:26037613-26037632	None:intergenic	40.0%
	TAGCAGTGGAATGGAATGTA+AGG	+	chr5.3:26037620-26037639	None:intergenic	40.0%
	TGGAATGGAATGTAAGGACA+TGG	+	chr5.3:26037614-26037633	None:intergenic	40.0%
!	CCCTGTTGCCATTTATATTG+TGG	+	chr5.3:26038074-26038093	None:intergenic	40.0%
!	TAAAGTGGTGGGAGTAAGAA+AGG	-	chr5.3:26038163-26038182	MS.gene58518:CDS	40.0%
	AAGTTGGCAATGAATGCACC+TGG	+	chr5.3:26038112-26038131	None:intergenic	45.0%
	GGGAGTTTCTCAAACATTCC+AGG	-	chr5.3:26038091-26038110	MS.gene58518:intron	45.0%
	GTGTGGACCTTGCAAAGTTA+TGG	-	chr5.3:26037799-26037818	MS.gene58518:intron	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	26037558	26038223	26037558	ID=MS.gene58518
chr5.3	mRNA	26037558	26038223	26037558	ID=MS.gene58518.t1;Parent=MS.gene58518
chr5.3	exon	26038098	26038223	26038098	ID=MS.gene58518.t1.exon1;Parent=MS.gene58518.t1
chr5.3	CDS	26038098	26038223	26038098	ID=cds.MS.gene58518.t1;Parent=MS.gene58518.t1
chr5.3	exon	26037886	26038008	26037886	ID=MS.gene58518.t1.exon2;Parent=MS.gene58518.t1
chr5.3	CDS	26037886	26038008	26037886	ID=cds.MS.gene58518.t1;Parent=MS.gene58518.t1
chr5.3	exon	26037558	26037689	26037558	ID=MS.gene58518.t1.exon3;Parent=MS.gene58518.t1
chr5.3	CDS	26037558	26037689	26037558	ID=cds.MS.gene58518.t1;Parent=MS.gene58518.t1

Gene Sequence

>MS.gene58518

ATGAGTTCCAACTTGTATAATTTTGTGTATGTTGATAGATCAACAGAATCATCCCATGTCCTTACATTCCATTCCACTGCTAAATGGAATGCTCATTTTGAAGCTTTCAAAGAAACTAATAAACTGGTATGTTTGCGTAGCTAAAATCTTAACTAGCTATAGAATCTTAGTATATAAAATTTAGTTTTCTTATGTAGTATTTTTCTTTGGTATAGATGGTTATTGATTTCACAGCTAAATGGTGTGGACCTTGCAAAGTTATGGATCCAGTTATAAAAGATTTTGCTGCAAAATACACTGATGTTGAGTTCATTAAGCTTGATGTTGATGAGTTATTGGTATGTTACAGATTATCCTTCACTTTAACTAGTTTGTTATTTATGTAAAACCAACTTTATGCCAGGTTTAATTACTCAGTTGGTTTCTAAACTATTTGAATTTTTTTGAACCTATTATAAATGCTATTATTTTTTAGAAACATGTTTTCAACACTTGAGAACTGATTTGTTTTTTCCACAATATAAATGGCAACAGGGAGTTTCTCAAACATTCCAGGTGCATTCATTGCCAACTTTTCTACTCATTAAGAAAGGAAAAGTTGTTGATAAAGTGGTGGGAGTAAGAAAGGAGCAACTACAAAGGATGATTGAAAAGCAAAGAAACTAA

Protein sequence

>MS.gene58518.t1

MSSNLYNFVYVDRSTESSHVLTFHSTAKWNAHFEAFKETNKLMVIDFTAKWCGPCKVMDPVIKDFAAKYTDVEFIKLDVDELLGVSQTFQVHSLPTFLLIKKGKVVDKVVGVRKEQLQRMIEKQRN