AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58643

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58643.t1 MTR_2g437700 95.309 405 12 2 1 405 12 409 0.0 759
MS.gene58643.t1 MTR_7g075453 32.759 348 214 11 16 361 23 352 1.29e-36 138
MS.gene58643.t1 MTR_2g018340 41.558 154 84 3 182 332 37 187 1.40e-31 119
MS.gene58643.t1 MTR_3g460780 28.840 319 213 10 16 333 31 336 8.70e-26 108
MS.gene58643.t1 MTR_5g098420 27.692 325 215 11 12 333 31 338 6.54e-22 97.4
MS.gene58643.t1 MTR_4g059840 31.718 227 143 6 183 406 72 289 2.52e-17 82.0
MS.gene58643.t1 MTR_7g101080 31.677 161 101 4 179 335 35 190 3.71e-17 81.3
MS.gene58643.t1 MTR_2g018380 36.066 122 72 3 230 345 53 174 1.55e-16 78.2
MS.gene58643.t1 MTR_5g098060 34.066 182 107 5 182 360 46 217 1.56e-16 79.3
MS.gene58643.t1 MTR_8g087460 27.948 229 150 6 183 405 92 311 7.41e-15 75.5
MS.gene58643.t1 MTR_8g087470 28.384 229 149 6 183 405 92 311 8.53e-15 75.1
MS.gene58643.t1 MTR_4g059790 30.939 181 115 5 183 360 72 245 2.08e-14 73.6
MS.gene58643.t1 MTR_2g093980 29.221 154 102 3 184 334 69 218 3.22e-14 73.2
MS.gene58643.t1 MTR_2g017950 28.718 195 124 5 179 364 68 256 4.22e-14 72.8
MS.gene58643.t1 MTR_4g053380 30.899 178 114 5 183 355 76 249 5.14e-14 72.4
MS.gene58643.t1 MTR_2g090765 29.730 185 121 5 153 334 8 186 7.38e-13 68.6
MS.gene58643.t1 MTR_2g017970 29.775 178 116 6 184 358 68 239 1.45e-12 68.2
MS.gene58643.t1 MTR_8g087450 31.544 149 95 4 184 329 77 221 1.85e-12 67.8
MS.gene58643.t1 MTR_2g017960 29.609 179 117 6 184 359 68 240 2.24e-12 67.4
MS.gene58643.t1 MTR_4g059720 27.778 162 108 4 174 333 35 189 3.21e-12 66.6
MS.gene58643.t1 MTR_2g093970 29.139 151 100 4 184 331 69 215 3.77e-12 67.0
MS.gene58643.t1 MTR_2g093990 28.947 152 102 3 184 333 41 188 7.20e-12 65.5
MS.gene58643.t1 MTR_1g009950 28.161 174 114 5 14 184 23 188 2.21e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58643.t1 AT3G46550 55.202 346 137 5 15 346 29 370 1.02e-119 354
MS.gene58643.t1 AT2G45470 33.333 324 202 10 14 336 25 335 1.95e-35 135
MS.gene58643.t1 AT3G60900 32.853 347 217 11 16 360 27 359 1.25e-30 122
MS.gene58643.t1 AT5G55730 29.538 325 213 11 10 333 20 329 8.62e-26 108
MS.gene58643.t1 AT5G55730 29.538 325 213 11 10 333 20 329 8.62e-26 108
MS.gene58643.t1 AT4G12730 28.704 324 214 12 12 333 24 332 6.03e-24 103
MS.gene58643.t1 AT5G44130 29.798 198 132 5 183 378 35 227 2.22e-18 84.3
MS.gene58643.t1 AT5G60490 31.579 152 99 3 184 333 39 187 2.59e-17 81.3
MS.gene58643.t1 AT1G03870 28.962 183 121 4 183 361 38 215 1.11e-15 76.6
MS.gene58643.t1 AT2G20520 31.646 158 97 5 183 334 37 189 4.32e-14 72.0
MS.gene58643.t1 AT5G03170 33.333 171 107 5 179 346 31 197 7.32e-14 71.2
MS.gene58643.t1 AT2G04780 30.380 158 99 4 183 336 45 195 1.84e-13 70.5
MS.gene58643.t1 AT2G04780 30.380 158 99 4 183 336 45 195 1.84e-13 70.5
MS.gene58643.t1 AT3G12660 31.210 157 103 3 10 166 19 170 6.67e-13 68.6

Find 150 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGATGTTGCAAGTGTTTA+TGG 0.107039 2.3:-53976697 MS.gene58643:CDS
TTGTAATGGTGTTGGTGTTC+GGG 0.251448 2.3:+53977469 None:intergenic
TCCTCCTCCAATTCCTTTCC+TGG 0.255019 2.3:-53977596 MS.gene58643:CDS
TTGTTGCCTAGAGAGATATT+TGG 0.295264 2.3:-53976862 MS.gene58643:CDS
GATTAACACTGGCATTGTTC+AGG 0.317584 2.3:-53976947 MS.gene58643:CDS
CTTTCTTCACCGCCGGGGTT+TGG 0.326429 2.3:-53976733 MS.gene58643:CDS
GAGGTGAATGTTGATAATTC+AGG 0.334884 2.3:+53977768 None:intergenic
GCTTCAATGCTCTCCGCTTC+CGG 0.361932 2.3:-53977246 MS.gene58643:CDS
GTGAGGGTCGGTGGTGAGAT+AGG 0.366350 2.3:+53977668 None:intergenic
GATGTCAGCGAGGGCGGAGG+GGG 0.371105 2.3:+53977632 None:intergenic
ATGGTGTTGGGGATAGGATT+AGG 0.379163 2.3:+53977829 None:intergenic
GTGAGCATCGATGAGGGTTT+TGG 0.379755 2.3:+53977284 None:intergenic
TGTTGCCTAGAGAGATATTT+GGG 0.387732 2.3:-53976861 MS.gene58643:CDS
ATTGTAATGGTGTTGGTGTT+CGG 0.388000 2.3:+53977468 None:intergenic
TGGTGTTGGTGTTCGGGGAG+TGG 0.394617 2.3:+53977475 None:intergenic
TGGTGGCCCGGCCGGTGGTT+TGG 0.397807 2.3:+53977523 None:intergenic
GGCGATTTCGCAGACGCAAT+TGG 0.405814 2.3:+53976832 None:intergenic
GGTGTTGGTGTTCGGGGAGT+GGG 0.411168 2.3:+53977476 None:intergenic
TGAAAGAGAAAGATGGTGTT+GGG 0.411336 2.3:+53977817 None:intergenic
CTTCGTGCTTATTATCCTCT+CGG 0.417892 2.3:-53977075 MS.gene58643:CDS
GGTCGGACCAGGAAAGGAAT+TGG 0.420438 2.3:+53977589 None:intergenic
GGCCGGGCCACCAACAACTT+TGG 0.425079 2.3:-53977513 MS.gene58643:CDS
GTCGGTGGTGAGATAGGTGT+TGG 0.428158 2.3:+53977674 None:intergenic
AAGAGTAAGGTGCTGGGGAA+TGG 0.428506 2.3:+53977445 None:intergenic
GAGGATGTCAGCGAGGGCGG+AGG 0.433140 2.3:+53977629 None:intergenic
GGGGAGAGGTTATGGAGGTG+AGG 0.434679 2.3:+53977651 None:intergenic
AAAGGAATTGGAGGAGGATG+TGG 0.434922 2.3:+53977601 None:intergenic
TGAAAGAACGGTGGCGTTTG+AGG 0.443446 2.3:+53977422 None:intergenic
GAAGCCGGTGAAGGTGGTTC+CGG 0.446950 2.3:-53977207 MS.gene58643:CDS
GTCACATCTTTCTTCACCGC+CGG 0.448378 2.3:-53976740 MS.gene58643:CDS
AGAAAGATGGTGTTGGGGAT+AGG 0.453694 2.3:+53977823 None:intergenic
GAGAGTGGTGACGAGTTTAC+CGG 0.456170 2.3:+53977548 None:intergenic
GAGGTGTTGGAGGTCGGACC+AGG 0.458257 2.3:+53977578 None:intergenic
TCGGCGAAAGCATCGTCTAC+GGG 0.459267 2.3:+53977168 None:intergenic
GGATGTCAGCGAGGGCGGAG+GGG 0.461161 2.3:+53977631 None:intergenic
CGGAGGGAGGGAGGTGTTGG+AGG 0.464496 2.3:+53977568 None:intergenic
CCCTTACGGTTTCGATCTCA+TGG 0.465462 2.3:-53977343 MS.gene58643:CDS
GATGCTGCTTCTTCCCCGGC+AGG 0.468798 2.3:-53976778 MS.gene58643:CDS
ATTAACACTGGCATTGTTCA+GGG 0.469145 2.3:-53976946 MS.gene58643:CDS
GGTGAAGATAGTGAGATTGT+AGG 0.469199 2.3:+53977383 None:intergenic
GACACTTGCGACGGAAGCTA+TGG 0.469462 2.3:-53977019 MS.gene58643:CDS
CTCTCTTCCAAACCACCGGC+CGG 0.475597 2.3:-53977530 MS.gene58643:CDS
TTCAGGGAAGGTTGAAAGGA+GGG 0.476637 2.3:+53977785 None:intergenic
AGGTGAATGTTGATAATTCA+GGG 0.477819 2.3:+53977769 None:intergenic
AGCCAAAGTTGTTGGTGGCC+CGG 0.478504 2.3:+53977511 None:intergenic
ATTGGAGGAGGATGTGGTAG+CGG 0.481178 2.3:+53977607 None:intergenic
TCACATCTTTCTTCACCGCC+GGG 0.484923 2.3:-53976739 MS.gene58643:CDS
GGGAGAGATCAGCGGTGAGA+GGG 0.486859 2.3:+53977727 None:intergenic
TCGGTGGTGAGATAGGTGTT+GGG 0.492560 2.3:+53977675 None:intergenic
TTTGAGGAAGAGTAAGGTGC+TGG 0.494680 2.3:+53977438 None:intergenic
AAAGTTGTTGGTGGCCCGGC+CGG 0.495578 2.3:+53977515 None:intergenic
TACCGGAGGGAGGGAGGTGT+TGG 0.497410 2.3:+53977565 None:intergenic
CGGGGAGTGGGTGATGTTGA+CGG 0.497837 2.3:+53977488 None:intergenic
TTCATTCACGACGCCGGAAG+CGG 0.499715 2.3:+53977233 None:intergenic
GTTGTTGGTGGCCCGGCCGG+TGG 0.501570 2.3:+53977518 None:intergenic
GGCTGTACATCAAAACCTGC+CGG 0.510148 2.3:+53976763 None:intergenic
AGGATGTCAGCGAGGGCGGA+GGG 0.513684 2.3:+53977630 None:intergenic
GTCGCAAGTGTCGGTTGCAA+CGG 0.515529 2.3:+53977030 None:intergenic
AGGTTGAAAGGAGGGAGGTG+AGG 0.516160 2.3:+53977793 None:intergenic
ATAATTCAGGGAAGGTTGAA+AGG 0.519692 2.3:+53977781 None:intergenic
ATCGGCGAAAGCATCGTCTA+CGG 0.521274 2.3:+53977167 None:intergenic
GGGGATAAACAAGGTGATAC+CGG 0.523824 2.3:+53977188 None:intergenic
CTCCAACACCTCCCTCCCTC+CGG 0.523989 2.3:-53977567 MS.gene58643:CDS
TTGAAAGAGAAAGATGGTGT+TGG 0.524791 2.3:+53977816 None:intergenic
GCGGAGGATGTCAGCGAGGG+CGG 0.525244 2.3:+53977626 None:intergenic
GCGACGGAAGCTATGGGTGC+TGG 0.525814 2.3:-53977012 MS.gene58643:CDS
GTTATGGAGGTGAGGGTCGG+TGG 0.527291 2.3:+53977659 None:intergenic
GAAGAAGCAGCATCCTCAAA+AGG 0.527700 2.3:+53976787 None:intergenic
GATACCGGAACCACCTTCAC+CGG 0.527970 2.3:+53977203 None:intergenic
GTGAAGATAGTGAGATTGTA+GGG 0.528446 2.3:+53977384 None:intergenic
GGGAGGGAGGTGTTGGAGGT+CGG 0.529487 2.3:+53977572 None:intergenic
GGAGGAGGAGCACTACTCTC+CGG 0.529688 2.3:+53976808 None:intergenic
ATTCAGGGAAGGTTGAAAGG+AGG 0.530201 2.3:+53977784 None:intergenic
TGGGAGAGATCAGCGGTGAG+AGG 0.533298 2.3:+53977726 None:intergenic
TCTCTTCCAAACCACCGGCC+GGG 0.536880 2.3:-53977529 MS.gene58643:CDS
AATGAATTCGAAGCCGGTGA+AGG 0.538116 2.3:-53977216 MS.gene58643:CDS
GGAGAGAGAGTGAATTGCGG+TGG 0.541485 2.3:+53977706 None:intergenic
GGAATGGATTGTAATGGTGT+TGG 0.543280 2.3:+53977461 None:intergenic
GCTGTACATCAAAACCTGCC+GGG 0.545192 2.3:+53976764 None:intergenic
GTTGACGGAGCCAAAGTTGT+TGG 0.548182 2.3:+53977503 None:intergenic
TGAGGATGCTGCTTCTTCCC+CGG 0.549925 2.3:-53976782 MS.gene58643:CDS
GGGAGAGGTTATGGAGGTGA+GGG 0.552715 2.3:+53977652 None:intergenic
TGCTGGGGAATGGATTGTAA+TGG 0.553637 2.3:+53977455 None:intergenic
TGTAATGGTGTTGGTGTTCG+GGG 0.554234 2.3:+53977470 None:intergenic
TTCTTCCCAAATATCTCTCT+AGG 0.555627 2.3:+53976856 None:intergenic
CGGTTTCGATCTCATGGCAT+CGG 0.555806 2.3:-53977337 MS.gene58643:CDS
TTCGAATTCATTCACGACGC+CGG 0.555834 2.3:+53977227 None:intergenic
GTGATGTTGAGAAGAATGCT+TGG 0.555902 2.3:+53977306 None:intergenic
GCCATGAGATCGAAACCGTA+AGG 0.558245 2.3:+53977342 None:intergenic
GTGGCGTTTGAGGAAGAGTA+AGG 0.559294 2.3:+53977432 None:intergenic
GAGGTTATGGAGGTGAGGGT+CGG 0.559779 2.3:+53977656 None:intergenic
GTGAGGTTGAAAGAGAAAGA+TGG 0.560128 2.3:+53977810 None:intergenic
ACCAGGAAAGGAATTGGAGG+AGG 0.566516 2.3:+53977595 None:intergenic
CGAAACGGAAGGAGGTAGAT+CGG 0.567781 2.3:+53977149 None:intergenic
TGCGTCTGCGAAATCGCCAC+CGG 0.570291 2.3:-53976827 MS.gene58643:CDS
TTGAGGAAGAGTAAGGTGCT+GGG 0.571039 2.3:+53977439 None:intergenic
GAGAGATCAGCGGTGAGAGG+GGG 0.573409 2.3:+53977729 None:intergenic
GGAGAGATCAGCGGTGAGAG+GGG 0.577869 2.3:+53977728 None:intergenic
GAATTCGAAGCCGGTGAAGG+TGG 0.580678 2.3:-53977213 MS.gene58643:CDS
GACGGAGCCAAAGTTGTTGG+TGG 0.580994 2.3:+53977506 None:intergenic
GTTGGAGGTCGGACCAGGAA+AGG 0.581865 2.3:+53977583 None:intergenic
GGCTCTGTTGCGATTAACAC+TGG 0.583502 2.3:-53976958 MS.gene58643:CDS
GAGAGAGAGTGAATTGCGGT+GGG 0.591977 2.3:+53977707 None:intergenic
GGAGGAGGATGTGGTAGCGG+AGG 0.593191 2.3:+53977610 None:intergenic
ACACTTGCGACGGAAGCTAT+GGG 0.597246 2.3:-53977018 MS.gene58643:CDS
GGGAAGTGATTGAAGCGAAA+CGG 0.597351 2.3:+53977134 None:intergenic
ACCACTCTCTTCCAAACCAC+CGG 0.598222 2.3:-53977534 MS.gene58643:CDS
GCAGCATCCTCAAAAGGAGG+AGG 0.601975 2.3:+53976793 None:intergenic
AGGGATTAAAAGAGAATCAA+CGG 0.602507 2.3:+53977362 None:intergenic
GAAATTGTGAGCATCGATGA+GGG 0.603493 2.3:+53977278 None:intergenic
GAATGTTGATAATTCAGGGA+AGG 0.603611 2.3:+53977773 None:intergenic
ATAGCTTCCGTCGCAAGTGT+CGG 0.604769 2.3:+53977021 None:intergenic
GGTGAGATAGGTGTTGGGGA+CGG 0.604958 2.3:+53977680 None:intergenic
TGACGAGTTTACCGGAGGGA+GGG 0.605757 2.3:+53977556 None:intergenic
TGAAATTGTGAGCATCGATG+AGG 0.610224 2.3:+53977277 None:intergenic
CGAGTTTACCGGAGGGAGGG+AGG 0.611461 2.3:+53977559 None:intergenic
AGTGATTGAAGCGAAACGGA+AGG 0.612745 2.3:+53977138 None:intergenic
GGCGGTGAAGAAAGATGTGA+CGG 0.614628 2.3:+53976742 None:intergenic
CGGTTGCAACGGATTCGCAG+TGG 0.617668 2.3:+53977041 None:intergenic
AGGGAAGGTTGAAAGGAGGG+AGG 0.618290 2.3:+53977788 None:intergenic
CGGTGGTGAGATAGGTGTTG+GGG 0.618735 2.3:+53977676 None:intergenic
GTCGTGAATGAATTCGAAGC+CGG 0.623460 2.3:-53977222 MS.gene58643:CDS
GCCGGTGGTTTGGAAGAGAG+TGG 0.626248 2.3:+53977533 None:intergenic
CGGCGAAAGCATCGTCTACG+GGG 0.626762 2.3:+53977169 None:intergenic
CGGACCAGGAAAGGAATTGG+AGG 0.630762 2.3:+53977592 None:intergenic
TTAACACTGGCATTGTTCAG+GGG 0.631498 2.3:-53976945 MS.gene58643:CDS
GTGACGAGTTTACCGGAGGG+AGG 0.632618 2.3:+53977555 None:intergenic
CCATGAGATCGAAACCGTAA+GGG 0.633243 2.3:+53977343 None:intergenic
CACATCTTTCTTCACCGCCG+GGG 0.635762 2.3:-53976738 MS.gene58643:CDS
GAAAGAGAAAGATGGTGTTG+GGG 0.638474 2.3:+53977818 None:intergenic
AGTGGTGACGAGTTTACCGG+AGG 0.642661 2.3:+53977551 None:intergenic
GGGTGTGGAAGAAAGTAGGG+AGG 0.644752 2.3:+53977749 None:intergenic
ATCGTCTACGGGGATAAACA+AGG 0.645330 2.3:+53977179 None:intergenic
CTGTACATCAAAACCTGCCG+GGG 0.646834 2.3:+53976765 None:intergenic
GTAGCGGAGGATGTCAGCGA+GGG 0.649494 2.3:+53977623 None:intergenic
GTGGATTGAAGCGAACCGAG+AGG 0.652533 2.3:+53977060 None:intergenic
TGAGGAAGAGTAAGGTGCTG+GGG 0.655459 2.3:+53977440 None:intergenic
GGAGGAGCACTACTCTCCGG+TGG 0.660835 2.3:+53976811 None:intergenic
GTGGTGACGAGTTTACCGGA+GGG 0.663417 2.3:+53977552 None:intergenic
GATAGGTGTTGGGGACGGCG+AGG 0.663521 2.3:+53977685 None:intergenic
GATTGAAGCGAAACGGAAGG+AGG 0.667934 2.3:+53977141 None:intergenic
GAAGCAGCATCCTCAAAAGG+AGG 0.671226 2.3:+53976790 None:intergenic
GTTGCAACCGACACTTGCGA+CGG 0.682389 2.3:-53977028 MS.gene58643:CDS
CTAACATCTTCACCAAACCC+CGG 0.698012 2.3:+53976721 None:intergenic
AATAGGATTCTGATCAAACA+CGG 0.698113 2.3:+53976909 None:intergenic
CGAGGAGAGAGAGTGAATTG+CGG 0.703819 2.3:+53977703 None:intergenic
ATTGCGGTGGGAGAGATCAG+CGG 0.710115 2.3:+53977719 None:intergenic
GGTAGCGGAGGATGTCAGCG+AGG 0.710162 2.3:+53977622 None:intergenic
TTTGAGCTGTGAAAGAACGG+TGG 0.719224 2.3:+53977413 None:intergenic
ACATCTTCACCAAACCCCGG+CGG 0.772064 2.3:+53976724 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAATATTTCAACTTTTAA+TGG - chr2.3:53977503-53977522 MS.gene58643:CDS 15.0%
AATAGGATTCTGATCAAACA+CGG + chr2.3:53977576-53977595 None:intergenic 30.0%
AGGGATTAAAAGAGAATCAA+CGG + chr2.3:53977123-53977142 None:intergenic 30.0%
GAAACACCAAAAATAGCAAT+AGG + chr2.3:53977593-53977612 None:intergenic 30.0%
! GATTCTCTTTTAATCCCTTA+CGG - chr2.3:53977125-53977144 MS.gene58643:CDS 30.0%
!! AGGTGAATGTTGATAATTCA+GGG + chr2.3:53976716-53976735 None:intergenic 30.0%
!!! AAGAACAACTGCTTTTTGAT+CGG + chr2.3:53977375-53977394 None:intergenic 30.0%
!!! CAGAATCCTATTGCTATTTT+TGG - chr2.3:53977584-53977603 MS.gene58643:CDS 30.0%
ATAATTCAGGGAAGGTTGAA+AGG + chr2.3:53976704-53976723 None:intergenic 35.0%
ATTAACACTGGCATTGTTCA+GGG - chr2.3:53977536-53977555 MS.gene58643:CDS 35.0%
GTGAAGATAGTGAGATTGTA+GGG + chr2.3:53977101-53977120 None:intergenic 35.0%
TCAGATGTTGCAAGTGTTTA+TGG - chr2.3:53977785-53977804 MS.gene58643:CDS 35.0%
TGAAAGAGAAAGATGGTGTT+GGG + chr2.3:53976668-53976687 None:intergenic 35.0%
TGTTGCCTAGAGAGATATTT+GGG - chr2.3:53977621-53977640 MS.gene58643:CDS 35.0%
TTCTTCCCAAATATCTCTCT+AGG + chr2.3:53977629-53977648 None:intergenic 35.0%
TTGAAAGAGAAAGATGGTGT+TGG + chr2.3:53976669-53976688 None:intergenic 35.0%
TTGTTGCCTAGAGAGATATT+TGG - chr2.3:53977620-53977639 MS.gene58643:CDS 35.0%
! CATTTTGAGCTGTGAAAGAA+CGG + chr2.3:53977075-53977094 None:intergenic 35.0%
!! ATTGTAATGGTGTTGGTGTT+CGG + chr2.3:53977017-53977036 None:intergenic 35.0%
!! GAATGTTGATAATTCAGGGA+AGG + chr2.3:53976712-53976731 None:intergenic 35.0%
!! GAGGTGAATGTTGATAATTC+AGG + chr2.3:53976717-53976736 None:intergenic 35.0%
!!! TGCTATTTTTGGTGTTTCGA+AGG - chr2.3:53977595-53977614 MS.gene58643:CDS 35.0%
CTTCGTGCTTATTATCCTCT+CGG - chr2.3:53977407-53977426 MS.gene58643:CDS 40.0%
GATTAACACTGGCATTGTTC+AGG - chr2.3:53977535-53977554 MS.gene58643:CDS 40.0%
GGTGAAGATAGTGAGATTGT+AGG + chr2.3:53977102-53977121 None:intergenic 40.0%
GTGAGGTTGAAAGAGAAAGA+TGG + chr2.3:53976675-53976694 None:intergenic 40.0%
GTGATGTTGAGAAGAATGCT+TGG + chr2.3:53977179-53977198 None:intergenic 40.0%
TTAACACTGGCATTGTTCAG+GGG - chr2.3:53977537-53977556 MS.gene58643:CDS 40.0%
! GAAAGAGAAAGATGGTGTTG+GGG + chr2.3:53976667-53976686 None:intergenic 40.0%
!! GAAATTGTGAGCATCGATGA+GGG + chr2.3:53977207-53977226 None:intergenic 40.0%
!! GGAATGGATTGTAATGGTGT+TGG + chr2.3:53977024-53977043 None:intergenic 40.0%
!! TGAAATTGTGAGCATCGATG+AGG + chr2.3:53977208-53977227 None:intergenic 40.0%
!! TTGTAATGGTGTTGGTGTTC+GGG + chr2.3:53977016-53977035 None:intergenic 40.0%
!!! AACAACTGCTTTTTGATCGG+CGG + chr2.3:53977372-53977391 None:intergenic 40.0%
AAAGGAATTGGAGGAGGATG+TGG + chr2.3:53976884-53976903 None:intergenic 45.0%
AATGAATTCGAAGCCGGTGA+AGG - chr2.3:53977266-53977285 MS.gene58643:CDS 45.0%
AGTGATTGAAGCGAAACGGA+AGG + chr2.3:53977347-53977366 None:intergenic 45.0%
ATCGTCTACGGGGATAAACA+AGG + chr2.3:53977306-53977325 None:intergenic 45.0%
ATTCAGGGAAGGTTGAAAGG+AGG + chr2.3:53976701-53976720 None:intergenic 45.0%
CCATGAGATCGAAACCGTAA+GGG + chr2.3:53977142-53977161 None:intergenic 45.0%
CTAACATCTTCACCAAACCC+CGG + chr2.3:53977764-53977783 None:intergenic 45.0%
GAAGAAGCAGCATCCTCAAA+AGG + chr2.3:53977698-53977717 None:intergenic 45.0%
GGGAAGTGATTGAAGCGAAA+CGG + chr2.3:53977351-53977370 None:intergenic 45.0%
GGGGATAAACAAGGTGATAC+CGG + chr2.3:53977297-53977316 None:intergenic 45.0%
GTCGTGAATGAATTCGAAGC+CGG - chr2.3:53977260-53977279 MS.gene58643:CDS 45.0%
TTCAGGGAAGGTTGAAAGGA+GGG + chr2.3:53976700-53976719 None:intergenic 45.0%
TTCGAATTCATTCACGACGC+CGG + chr2.3:53977258-53977277 None:intergenic 45.0%
TTTGAGCTGTGAAAGAACGG+TGG + chr2.3:53977072-53977091 None:intergenic 45.0%
TTTGAGGAAGAGTAAGGTGC+TGG + chr2.3:53977047-53977066 None:intergenic 45.0%
! AGAAAGATGGTGTTGGGGAT+AGG + chr2.3:53976662-53976681 None:intergenic 45.0%
! ATGGTGTTGGGGATAGGATT+AGG + chr2.3:53976656-53976675 None:intergenic 45.0%
! TTGAGGAAGAGTAAGGTGCT+GGG + chr2.3:53977046-53977065 None:intergenic 45.0%
!! TGCTGGGGAATGGATTGTAA+TGG + chr2.3:53977030-53977049 None:intergenic 45.0%
!! TGTAATGGTGTTGGTGTTCG+GGG + chr2.3:53977015-53977034 None:intergenic 45.0%
!!! ACAACTGCTTTTTGATCGGC+GGG + chr2.3:53977371-53977390 None:intergenic 45.0%
ACACTTGCGACGGAAGCTAT+GGG - chr2.3:53977464-53977483 MS.gene58643:CDS 50.0%
ACCACTCTCTTCCAAACCAC+CGG - chr2.3:53976948-53976967 MS.gene58643:CDS 50.0%
ACCAGGAAAGGAATTGGAGG+AGG + chr2.3:53976890-53976909 None:intergenic 50.0%
ATAGCTTCCGTCGCAAGTGT+CGG + chr2.3:53977464-53977483 None:intergenic 50.0%
ATTGGAGGAGGATGTGGTAG+CGG + chr2.3:53976878-53976897 None:intergenic 50.0%
CCCTTACGGTTTCGATCTCA+TGG - chr2.3:53977139-53977158 MS.gene58643:CDS 50.0%
CGAAACGGAAGGAGGTAGAT+CGG + chr2.3:53977336-53977355 None:intergenic 50.0%
CGAGGAGAGAGAGTGAATTG+CGG + chr2.3:53976782-53976801 None:intergenic 50.0%
CGGTTTCGATCTCATGGCAT+CGG - chr2.3:53977145-53977164 MS.gene58643:CDS 50.0%
CTGTACATCAAAACCTGCCG+GGG + chr2.3:53977720-53977739 None:intergenic 50.0%
GAAGCAGCATCCTCAAAAGG+AGG + chr2.3:53977695-53977714 None:intergenic 50.0%
GAGAGAGAGTGAATTGCGGT+GGG + chr2.3:53976778-53976797 None:intergenic 50.0%
GATTGAAGCGAAACGGAAGG+AGG + chr2.3:53977344-53977363 None:intergenic 50.0%
GCCATGAGATCGAAACCGTA+AGG + chr2.3:53977143-53977162 None:intergenic 50.0%
GCTGTACATCAAAACCTGCC+GGG + chr2.3:53977721-53977740 None:intergenic 50.0%
GGCGGTGAAGAAAGATGTGA+CGG + chr2.3:53977743-53977762 None:intergenic 50.0%
GGCTCTGTTGCGATTAACAC+TGG - chr2.3:53977524-53977543 MS.gene58643:CDS 50.0%
GGCTGTACATCAAAACCTGC+CGG + chr2.3:53977722-53977741 None:intergenic 50.0%
GTCACATCTTTCTTCACCGC+CGG - chr2.3:53977742-53977761 MS.gene58643:CDS 50.0%
GTGGCGTTTGAGGAAGAGTA+AGG + chr2.3:53977053-53977072 None:intergenic 50.0%
GTTGACGGAGCCAAAGTTGT+TGG + chr2.3:53976982-53977001 None:intergenic 50.0%
TCACATCTTTCTTCACCGCC+GGG - chr2.3:53977743-53977762 MS.gene58643:CDS 50.0%
TCCTCCTCCAATTCCTTTCC+TGG - chr2.3:53976886-53976905 MS.gene58643:CDS 50.0%
TCGGTGGTGAGATAGGTGTT+GGG + chr2.3:53976810-53976829 None:intergenic 50.0%
TGAAAGAACGGTGGCGTTTG+AGG + chr2.3:53977063-53977082 None:intergenic 50.0%
! AGGGGGTGTGGAAGAAAGTA+GGG + chr2.3:53976739-53976758 None:intergenic 50.0%
! GAGAGTGGTGACGAGTTTAC+CGG + chr2.3:53976937-53976956 None:intergenic 50.0%
! TAGTGCTCCTCCTCCTTTTG+AGG - chr2.3:53977682-53977701 MS.gene58643:CDS 50.0%
!! AAGAGTAAGGTGCTGGGGAA+TGG + chr2.3:53977040-53977059 None:intergenic 50.0%
!! ATCGGCGAAAGCATCGTCTA+CGG + chr2.3:53977318-53977337 None:intergenic 50.0%
!! TGAGGAAGAGTAAGGTGCTG+GGG + chr2.3:53977045-53977064 None:intergenic 50.0%
!!! GTGAGCATCGATGAGGGTTT+TGG + chr2.3:53977201-53977220 None:intergenic 50.0%
ACATCTTCACCAAACCCCGG+CGG + chr2.3:53977761-53977780 None:intergenic 55.0%
AGGGAAGGTTGAAAGGAGGG+AGG + chr2.3:53976697-53976716 None:intergenic 55.0%
AGGTTGAAAGGAGGGAGGTG+AGG + chr2.3:53976692-53976711 None:intergenic 55.0%
ATTGCGGTGGGAGAGATCAG+CGG + chr2.3:53976766-53976785 None:intergenic 55.0%
CACATCTTTCTTCACCGCCG+GGG - chr2.3:53977744-53977763 MS.gene58643:CDS 55.0%
CGGACCAGGAAAGGAATTGG+AGG + chr2.3:53976893-53976912 None:intergenic 55.0%
GAATTCGAAGCCGGTGAAGG+TGG - chr2.3:53977269-53977288 MS.gene58643:CDS 55.0%
GACACTTGCGACGGAAGCTA+TGG - chr2.3:53977463-53977482 MS.gene58643:CDS 55.0%
GACGGAGCCAAAGTTGTTGG+TGG + chr2.3:53976979-53976998 None:intergenic 55.0%
GAGGTTATGGAGGTGAGGGT+CGG + chr2.3:53976829-53976848 None:intergenic 55.0%
GATACCGGAACCACCTTCAC+CGG + chr2.3:53977282-53977301 None:intergenic 55.0%
GCAGCATCCTCAAAAGGAGG+AGG + chr2.3:53977692-53977711 None:intergenic 55.0%
GCTTCAATGCTCTCCGCTTC+CGG - chr2.3:53977236-53977255 MS.gene58643:CDS 55.0%
GGAGAGAGAGTGAATTGCGG+TGG + chr2.3:53976779-53976798 None:intergenic 55.0%
GGCGATTTCGCAGACGCAAT+TGG + chr2.3:53977653-53977672 None:intergenic 55.0%
GGGAGAGGTTATGGAGGTGA+GGG + chr2.3:53976833-53976852 None:intergenic 55.0%
GGGTGTGGAAGAAAGTAGGG+AGG + chr2.3:53976736-53976755 None:intergenic 55.0%
GGTCGGACCAGGAAAGGAAT+TGG + chr2.3:53976896-53976915 None:intergenic 55.0%
GTCGGTGGTGAGATAGGTGT+TGG + chr2.3:53976811-53976830 None:intergenic 55.0%
GTTGCAACCGACACTTGCGA+CGG - chr2.3:53977454-53977473 MS.gene58643:CDS 55.0%
TGACGAGTTTACCGGAGGGA+GGG + chr2.3:53976929-53976948 None:intergenic 55.0%
TTCATTCACGACGCCGGAAG+CGG + chr2.3:53977252-53977271 None:intergenic 55.0%
! AGTGGTGACGAGTTTACCGG+AGG + chr2.3:53976934-53976953 None:intergenic 55.0%
! CGGTGGTGAGATAGGTGTTG+GGG + chr2.3:53976809-53976828 None:intergenic 55.0%
! GAGGGGGTGTGGAAGAAAGT+AGG + chr2.3:53976740-53976759 None:intergenic 55.0%
! GTGGATTGAAGCGAACCGAG+AGG + chr2.3:53977425-53977444 None:intergenic 55.0%
! GTGGTGACGAGTTTACCGGA+GGG + chr2.3:53976933-53976952 None:intergenic 55.0%
! TGAGGATGCTGCTTCTTCCC+CGG - chr2.3:53977700-53977719 MS.gene58643:CDS 55.0%
!! AGCCAAAGTTGTTGGTGGCC+CGG + chr2.3:53976974-53976993 None:intergenic 55.0%
!! GGTGAGATAGGTGTTGGGGA+CGG + chr2.3:53976805-53976824 None:intergenic 55.0%
!! GTCGCAAGTGTCGGTTGCAA+CGG + chr2.3:53977455-53977474 None:intergenic 55.0%
!! TCGGCGAAAGCATCGTCTAC+GGG + chr2.3:53977317-53977336 None:intergenic 55.0%
CGGGGAGTGGGTGATGTTGA+CGG + chr2.3:53976997-53977016 None:intergenic 60.0%
CTCTCTTCCAAACCACCGGC+CGG - chr2.3:53976952-53976971 MS.gene58643:CDS 60.0%
CTTTCTTCACCGCCGGGGTT+TGG - chr2.3:53977749-53977768 MS.gene58643:CDS 60.0%
GAAGCCGGTGAAGGTGGTTC+CGG - chr2.3:53977275-53977294 MS.gene58643:CDS 60.0%
GAGAGATCAGCGGTGAGAGG+GGG + chr2.3:53976756-53976775 None:intergenic 60.0%
GGAGAGATCAGCGGTGAGAG+GGG + chr2.3:53976757-53976776 None:intergenic 60.0%
GGGAGAGATCAGCGGTGAGA+GGG + chr2.3:53976758-53976777 None:intergenic 60.0%
GGGGAGAGGTTATGGAGGTG+AGG + chr2.3:53976834-53976853 None:intergenic 60.0%
GTGACGAGTTTACCGGAGGG+AGG + chr2.3:53976930-53976949 None:intergenic 60.0%
GTGAGGGTCGGTGGTGAGAT+AGG + chr2.3:53976817-53976836 None:intergenic 60.0%
GTTATGGAGGTGAGGGTCGG+TGG + chr2.3:53976826-53976845 None:intergenic 60.0%
GTTGGAGGTCGGACCAGGAA+AGG + chr2.3:53976902-53976921 None:intergenic 60.0%
TCTCTTCCAAACCACCGGCC+GGG - chr2.3:53976953-53976972 MS.gene58643:CDS 60.0%
TGCGTCTGCGAAATCGCCAC+CGG - chr2.3:53977655-53977674 MS.gene58643:CDS 60.0%
TGGGAGAGATCAGCGGTGAG+AGG + chr2.3:53976759-53976778 None:intergenic 60.0%
! CGGTTGCAACGGATTCGCAG+TGG + chr2.3:53977444-53977463 None:intergenic 60.0%
! GCCGGTGGTTTGGAAGAGAG+TGG + chr2.3:53976952-53976971 None:intergenic 60.0%
! GTAGCGGAGGATGTCAGCGA+GGG + chr2.3:53976862-53976881 None:intergenic 60.0%
!! AAAGTTGTTGGTGGCCCGGC+CGG + chr2.3:53976970-53976989 None:intergenic 60.0%
!! CGGCGAAAGCATCGTCTACG+GGG + chr2.3:53977316-53977335 None:intergenic 60.0%
!! GGAGGAGGAGCACTACTCTC+CGG + chr2.3:53977677-53977696 None:intergenic 60.0%
!! GGTGTTGGTGTTCGGGGAGT+GGG + chr2.3:53977009-53977028 None:intergenic 60.0%
!! TGGTGTTGGTGTTCGGGGAG+TGG + chr2.3:53977010-53977029 None:intergenic 60.0%
AGGATGTCAGCGAGGGCGGA+GGG + chr2.3:53976855-53976874 None:intergenic 65.0%
ATCAGCGGTGAGAGGGGGTG+TGG + chr2.3:53976751-53976770 None:intergenic 65.0%
CGAGTTTACCGGAGGGAGGG+AGG + chr2.3:53976926-53976945 None:intergenic 65.0%
CGGAGGGGGAGAGGTTATGG+AGG + chr2.3:53976839-53976858 None:intergenic 65.0%
CTCCAACACCTCCCTCCCTC+CGG - chr2.3:53976915-53976934 MS.gene58643:CDS 65.0%
GCGACGGAAGCTATGGGTGC+TGG - chr2.3:53977470-53977489 MS.gene58643:CDS 65.0%
GGCCGGGCCACCAACAACTT+TGG - chr2.3:53976969-53976988 MS.gene58643:CDS 65.0%
! GATGCTGCTTCTTCCCCGGC+AGG - chr2.3:53977704-53977723 MS.gene58643:CDS 65.0%
! TACCGGAGGGAGGGAGGTGT+TGG + chr2.3:53976920-53976939 None:intergenic 65.0%
!! GAGGTGTTGGAGGTCGGACC+AGG + chr2.3:53976907-53976926 None:intergenic 65.0%
!! GATAGGTGTTGGGGACGGCG+AGG + chr2.3:53976800-53976819 None:intergenic 65.0%
!! GGAGGAGCACTACTCTCCGG+TGG + chr2.3:53977674-53977693 None:intergenic 65.0%
!! GGAGGAGGATGTGGTAGCGG+AGG + chr2.3:53976875-53976894 None:intergenic 65.0%
!! GGGAGGGAGGTGTTGGAGGT+CGG + chr2.3:53976913-53976932 None:intergenic 65.0%
!! GGTAGCGGAGGATGTCAGCG+AGG + chr2.3:53976863-53976882 None:intergenic 65.0%
GAGGATGTCAGCGAGGGCGG+AGG + chr2.3:53976856-53976875 None:intergenic 70.0%
GATGTCAGCGAGGGCGGAGG+GGG + chr2.3:53976853-53976872 None:intergenic 70.0%
GCGGAGGATGTCAGCGAGGG+CGG + chr2.3:53976859-53976878 None:intergenic 70.0%
GGATGTCAGCGAGGGCGGAG+GGG + chr2.3:53976854-53976873 None:intergenic 70.0%
GGGCGGAGGGGGAGAGGTTA+TGG + chr2.3:53976842-53976861 None:intergenic 70.0%
!! CGGAGGGAGGGAGGTGTTGG+AGG + chr2.3:53976917-53976936 None:intergenic 70.0%
TGGTGGCCCGGCCGGTGGTT+TGG + chr2.3:53976962-53976981 None:intergenic 75.0%
!! GTTGTTGGTGGCCCGGCCGG+TGG + chr2.3:53976967-53976986 None:intergenic 75.0%
! CAGCGAGGGCGGAGGGGGAG+AGG + chr2.3:53976848-53976867 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 53976642 53977862 53976642 ID=MS.gene58643
chr2.3 mRNA 53976642 53977862 53976642 ID=MS.gene58643.t1;Parent=MS.gene58643
chr2.3 exon 53976642 53977862 53976642 ID=MS.gene58643.t1.exon1;Parent=MS.gene58643.t1
chr2.3 CDS 53976642 53977862 53976642 ID=cds.MS.gene58643.t1;Parent=MS.gene58643.t1
Gene Sequence

>MS.gene58643

ATGCTATATTTCCTAATCCTATCCCCAACACCATCTTTCTCTTTCAACCTCACCTCCCTCCTTTCAACCTTCCCTGAATTATCAACATTCACCTCCCTACTTTCTTCCACACCCCCTCTCACCGCTGATCTCTCCCACCGCAATTCACTCTCTCTCCTCGCCGTCCCCAACACCTATCTCACCACCGACCCTCACCTCCATAACCTCTCCCCCTCCGCCCTCGCTGACATCCTCCGCTACCACATCCTCCTCCAATTCCTTTCCTGGTCCGACCTCCAACACCTCCCTCCCTCCGGTAAACTCGTCACCACTCTCTTCCAAACCACCGGCCGGGCCACCAACAACTTTGGCTCCGTCAACATCACCCACTCCCCGAACACCAACACCATTACAATCCATTCCCCAGCACCTTACTCTTCCTCAAACGCCACCGTTCTTTCACAGCTCAAAATGTTACCCTACAATCTCACTATCTTCACCGTTGATTCTCTTTTAATCCCTTACGGTTTCGATCTCATGGCATCGGAAACTCGTCCAAGCATTCTTCTCAACATCACCAAAACCCTCATCGATGCTCACAATTTCAACGTCGCTGCTTCAATGCTCTCCGCTTCCGGCGTCGTGAATGAATTCGAAGCCGGTGAAGGTGGTTCCGGTATCACCTTGTTTATCCCCGTAGACGATGCTTTCGCCGATCTACCTCCTTCCGTTTCGCTTCAATCACTTCCCGCCGATCAAAAAGCAGTTGTTCTTAAAGCGCATGTTCTTCGTGCTTATTATCCTCTCGGTTCGCTTCAATCCACTGCGAATCCGTTGCAACCGACACTTGCGACGGAAGCTATGGGTGCTGGAAGTTTCACGTTGAATATTTCAACTTTTAATGGCTCTGTTGCGATTAACACTGGCATTGTTCAGGGGATAATTACTCAGACCGTGTTTGATCAGAATCCTATTGCTATTTTTGGTGTTTCGAAGGTTTTGTTGCCTAGAGAGATATTTGGGAAGAATCCAATTGCGTCTGCGAAATCGCCACCGGAGAGTAGTGCTCCTCCTCCTTTTGAGGATGCTGCTTCTTCCCCGGCAGGTTTTGATGTACAGCCGTCACATCTTTCTTCACCGCCGGGGTTTGGTGAAGATGTTAGCTCAGATGTTGCAAGTGTTTATGGTTCTGAGTTATTTGTTTTTCTACTTTGTTGTATAAATTTGTATTTAATAGTATAA
Protein sequence

>MS.gene58643.t1

MLYFLILSPTPSFSFNLTSLLSTFPELSTFTSLLSSTPPLTADLSHRNSLSLLAVPNTYLTTDPHLHNLSPSALADILRYHILLQFLSWSDLQHLPPSGKLVTTLFQTTGRATNNFGSVNITHSPNTNTITIHSPAPYSSSNATVLSQLKMLPYNLTIFTVDSLLIPYGFDLMASETRPSILLNITKTLIDAHNFNVAASMLSASGVVNEFEAGEGGSGITLFIPVDDAFADLPPSVSLQSLPADQKAVVLKAHVLRAYYPLGSLQSTANPLQPTLATEAMGAGSFTLNISTFNGSVAINTGIVQGIITQTVFDQNPIAIFGVSKVLLPREIFGKNPIASAKSPPESSAPPPFEDAASSPAGFDVQPSHLSSPPGFGEDVSSDVASVYGSELFVFLLCCINLYLIV