Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58865.t1 | KEH39385.1 | 62.5 | 80 | 19 | 1 | 19 | 87 | 375 | 454 | 1.90E-13 | 85.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58865.t1 | Q9LHB1 | 50.0 | 58 | 29 | 0 | 22 | 79 | 380 | 437 | 1.9e-06 | 53.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58865.t1 | A0A072VMP6 | 62.5 | 80 | 19 | 1 | 19 | 87 | 375 | 454 | 1.3e-13 | 85.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene58865 | 0.805721 | 1.16E-49 | -1.69E-46 |
| MS.gene05288 | MS.gene58865 | 0.92507 | 3.02E-90 | -1.69E-46 |
| MS.gene05289 | MS.gene58865 | 0.912414 | 2.00E-83 | -1.69E-46 |
| MS.gene053584 | MS.gene58865 | 0.802588 | 5.22E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene58865 | 0.812519 | 4.07E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58865.t1 | MTR_4g126200 | 66.000 | 50 | 17 | 0 | 38 | 87 | 3 | 52 | 4.59e-13 | 62.8 |
| MS.gene58865.t1 | MTR_2g095400 | 68.750 | 64 | 20 | 0 | 19 | 82 | 375 | 438 | 3.30e-12 | 60.8 |
| MS.gene58865.t1 | MTR_5g079940 | 50.000 | 62 | 31 | 0 | 19 | 80 | 859 | 920 | 3.40e-12 | 60.8 |
| MS.gene58865.t1 | MTR_2g097010 | 67.188 | 64 | 21 | 0 | 19 | 82 | 401 | 464 | 4.10e-11 | 57.8 |
| MS.gene58865.t1 | MTR_3g067815 | 50.820 | 61 | 30 | 0 | 22 | 82 | 382 | 442 | 6.99e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58865.t1 | AT4G01180 | 49.180 | 61 | 31 | 0 | 16 | 76 | 277 | 337 | 7.10e-11 | 57.4 |
Find 14 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGATGCAAGACATGCTT+TGG | 0.365400 | 6.2:+78883227 | MS.gene58865:CDS |
| TGCAAGACATGCTTTGGAAT+TGG | 0.380086 | 6.2:+78883233 | MS.gene58865:CDS |
| AAAGAAGAAATGGCGGAAGC+TGG | 0.447708 | 6.2:+78882965 | None:intergenic |
| ATTGCATCACTTATCTCGTC+TGG | 0.458810 | 6.2:-78883010 | None:intergenic |
| TTGGATGTTGCAGAAAGAAA+AGG | 0.471032 | 6.2:+78883167 | MS.gene58865:intron |
| GTGTGTTTGTAGTGGAGACT+TGG | 0.486501 | 6.2:+78883392 | MS.gene58865:intron |
| AAGAAGAAATGGCGGAAGCT+GGG | 0.487627 | 6.2:+78882966 | None:intergenic |
| TGCAATATCAGATCAACAAA+AGG | 0.494458 | 6.2:+78883027 | MS.gene58865:CDS |
| GCGTTTGGCTGAAGACCATA+AGG | 0.535802 | 6.2:+78883066 | MS.gene58865:CDS |
| ATATCAGATCAACAAAAGGC+TGG | 0.578847 | 6.2:+78883031 | MS.gene58865:CDS |
| AGAAGAAATGGCGGAAGCTG+GGG | 0.585508 | 6.2:+78882967 | None:intergenic |
| AGAAATGGCGGAAGCTGGGG+TGG | 0.586264 | 6.2:+78882970 | None:intergenic |
| TCACAAGAAAATGCATGACC+TGG | 0.631525 | 6.2:+78883197 | MS.gene58865:CDS |
| TTGGAGATAGAACAGCTGAA+AGG | 0.635079 | 6.2:+78883252 | MS.gene58865:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTTATTTATATTTAGTAAT+GGG | + | chr6.2:78883364-78883383 | MS.gene58865:intron | 10.0% |
| !!! | AATTAAAAAATGTTGATAAA+AGG | - | chr6.2:78883107-78883126 | None:intergenic | 10.0% |
| !! | GACTTATTTATATTTAGTAA+TGG | + | chr6.2:78883363-78883382 | MS.gene58865:intron | 15.0% |
| !! | ACTAAATATAAATAAGTCGT+GGG | - | chr6.2:78883361-78883380 | None:intergenic | 20.0% |
| !! | TACTAAATATAAATAAGTCG+TGG | - | chr6.2:78883362-78883381 | None:intergenic | 20.0% |
| ! | AAAATCAAATGTGTACCTTA+TGG | - | chr6.2:78883084-78883103 | None:intergenic | 25.0% |
| !!! | TTATATTTAGTAATGGGTGT+TGG | + | chr6.2:78883370-78883389 | MS.gene58865:intron | 25.0% |
| !!! | TTATGTTTTGATGACTCTTT+TGG | + | chr6.2:78883144-78883163 | MS.gene58865:intron | 25.0% |
| AAGAGTCATCAAAACATAAG+TGG | - | chr6.2:78883143-78883162 | None:intergenic | 30.0% | |
| GAGGAATTAATATAGCAAGT+AGG | + | chr6.2:78883333-78883352 | MS.gene58865:intron | 30.0% | |
| TGCAATATCAGATCAACAAA+AGG | + | chr6.2:78883027-78883046 | MS.gene58865:CDS | 30.0% | |
| TTTCCGTGTAATGAAAAAAC+TGG | + | chr6.2:78883278-78883297 | MS.gene58865:CDS | 30.0% | |
| ATATCAGATCAACAAAAGGC+TGG | + | chr6.2:78883031-78883050 | MS.gene58865:CDS | 35.0% | |
| TGGTGAAAAAATGATGCGTT+TGG | + | chr6.2:78883051-78883070 | MS.gene58865:CDS | 35.0% | |
| TTGGATGTTGCAGAAAGAAA+AGG | + | chr6.2:78883167-78883186 | MS.gene58865:intron | 35.0% | |
| !! | GTTTTGATGACTCTTTTGGT+TGG | + | chr6.2:78883148-78883167 | MS.gene58865:intron | 35.0% |
| !!! | CCTCCAGTTTTTTCATTACA+CGG | - | chr6.2:78883284-78883303 | None:intergenic | 35.0% |
| ACTTGATGCAAGACATGCTT+TGG | + | chr6.2:78883227-78883246 | MS.gene58865:CDS | 40.0% | |
| ATTGCATCACTTATCTCGTC+TGG | - | chr6.2:78883013-78883032 | None:intergenic | 40.0% | |
| CCGTGTAATGAAAAAACTGG+AGG | + | chr6.2:78883281-78883300 | MS.gene58865:CDS | 40.0% | |
| GAAGCTGCATGAAAAGTAAG+AGG | + | chr6.2:78883314-78883333 | MS.gene58865:intron | 40.0% | |
| TCACAAGAAAATGCATGACC+TGG | + | chr6.2:78883197-78883216 | MS.gene58865:CDS | 40.0% | |
| TTGGAGATAGAACAGCTGAA+AGG | + | chr6.2:78883252-78883271 | MS.gene58865:CDS | 40.0% | |
| ! | TTGCATCAAGTCTTTTCACC+AGG | - | chr6.2:78883218-78883237 | None:intergenic | 40.0% |
| !! | TGCAAGACATGCTTTGGAAT+TGG | + | chr6.2:78883233-78883252 | MS.gene58865:CDS | 40.0% |
| ! | GTGTGTTTGTAGTGGAGACT+TGG | + | chr6.2:78883392-78883411 | MS.gene58865:intron | 45.0% |
| GCGTTTGGCTGAAGACCATA+AGG | + | chr6.2:78883066-78883085 | MS.gene58865:CDS | 50.0% | |
| !! | GGGTGTTGGTGTGTTTGTAG+TGG | + | chr6.2:78883384-78883403 | MS.gene58865:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 78882974 | 78883422 | 78882974 | ID=MS.gene58865 |
| chr6.2 | mRNA | 78882974 | 78883422 | 78882974 | ID=MS.gene58865.t1;Parent=MS.gene58865 |
| chr6.2 | exon | 78882974 | 78883087 | 78882974 | ID=MS.gene58865.t1.exon1;Parent=MS.gene58865.t1 |
| chr6.2 | CDS | 78882974 | 78883087 | 78882974 | ID=cds.MS.gene58865.t1;Parent=MS.gene58865.t1 |
| chr6.2 | exon | 78883180 | 78883328 | 78883180 | ID=MS.gene58865.t1.exon2;Parent=MS.gene58865.t1 |
| chr6.2 | CDS | 78883180 | 78883328 | 78883180 | ID=cds.MS.gene58865.t1;Parent=MS.gene58865.t1 |
| chr6.2 | exon | 78883404 | 78883422 | 78883404 | ID=MS.gene58865.t1.exon3;Parent=MS.gene58865.t1 |
| chr6.2 | CDS | 78883404 | 78883422 | 78883404 | ID=cds.MS.gene58865.t1;Parent=MS.gene58865.t1 |
| Gene Sequence |
| Protein sequence |
