Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59837.t1 | XP_013463738.1 | 70.6 | 119 | 4 | 2 | 1 | 88 | 1 | 119 | 3.80E-32 | 147.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59837.t1 | Q9SPE5 | 54.6 | 119 | 19 | 2 | 3 | 88 | 2 | 118 | 3.3e-24 | 112.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59837.t1 | B7FIS3 | 70.6 | 119 | 4 | 2 | 1 | 88 | 1 | 119 | 2.7e-32 | 147.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene59837 | 0.83814 | 3.43E-57 | -1.69E-46 |
| MS.gene051198 | MS.gene59837 | 0.806392 | 8.41E-50 | -1.69E-46 |
| MS.gene051210 | MS.gene59837 | 0.802102 | 6.58E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene59837 | 0.806963 | 6.37E-50 | -1.69E-46 |
| MS.gene053079 | MS.gene59837 | 0.822813 | 1.94E-53 | -1.69E-46 |
| MS.gene054236 | MS.gene59837 | 0.801713 | 7.91E-49 | -1.69E-46 |
| MS.gene056507 | MS.gene59837 | 0.802495 | 5.46E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene59837 | 0.801905 | 7.22E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene59837 | 0.809702 | 1.66E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene59837 | 0.802115 | 6.54E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59837.t1 | MTR_2g047110 | 70.588 | 119 | 4 | 2 | 1 | 88 | 1 | 119 | 9.00e-48 | 153 |
| MS.gene59837.t1 | MTR_2g047110 | 66.019 | 103 | 4 | 2 | 17 | 88 | 9 | 111 | 3.44e-36 | 123 |
| MS.gene59837.t1 | MTR_8g089250 | 51.240 | 121 | 24 | 2 | 1 | 88 | 1 | 119 | 4.30e-32 | 113 |
| MS.gene59837.t1 | MTR_2g047110 | 100.000 | 49 | 0 | 0 | 40 | 88 | 8 | 56 | 3.41e-29 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59837.t1 | AT4G20410 | 54.622 | 119 | 19 | 2 | 3 | 88 | 2 | 118 | 2.62e-33 | 116 |
| MS.gene59837.t1 | AT4G20410 | 53.774 | 106 | 14 | 2 | 16 | 88 | 21 | 124 | 4.22e-26 | 97.8 |
Find 27 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATACAGTTTATCAGCTT+TGG | 0.316478 | 8.3:-54188190 | None:intergenic |
| TGTTCTTGTTCTTTATGAAC+AGG | 0.358291 | 8.3:+54188523 | MS.gene59837:CDS |
| CATGGAATCTGCCGCTGCTC+TGG | 0.386852 | 8.3:+54189120 | MS.gene59837:CDS |
| GATGCTCTTGCAAAGGGTGC+TGG | 0.393013 | 8.3:+54189235 | MS.gene59837:CDS |
| TGGCTAAGGAACTGAGCAAC+TGG | 0.441808 | 8.3:+54189140 | MS.gene59837:CDS |
| AGCTTTGGCTAACAACTTGT+TGG | 0.442140 | 8.3:-54188175 | None:intergenic |
| TCACTAGGTGGAGTGTTGAT+TGG | 0.452980 | 8.3:+54188489 | MS.gene59837:CDS |
| GGCTAACAACTTGTTGGGAT+CGG | 0.471288 | 8.3:-54188169 | None:intergenic |
| GCTTTGGCTAACAACTTGTT+GGG | 0.472378 | 8.3:-54188174 | None:intergenic |
| ATCTGCCGCTGCTCTGGCTA+AGG | 0.488319 | 8.3:+54189126 | MS.gene59837:CDS |
| CTTGTTCTTTATGAACAGGC+TGG | 0.494733 | 8.3:+54188527 | MS.gene59837:CDS |
| GCATCGGATGCTCTTGCAAA+GGG | 0.506565 | 8.3:+54189229 | MS.gene59837:CDS |
| TTTGCAAGAGCATCCGATGC+AGG | 0.511747 | 8.3:-54189226 | None:intergenic |
| AGAGCATCCGATGCAGGTTG+TGG | 0.518339 | 8.3:-54189220 | None:intergenic |
| ATGCTCTTGCAAAGGGTGCT+GGG | 0.535295 | 8.3:+54189236 | MS.gene59837:CDS |
| CAACTTGTTGGGATCGGAGG+AGG | 0.551426 | 8.3:-54188163 | None:intergenic |
| TGCATCGGATGCTCTTGCAA+AGG | 0.564233 | 8.3:+54189228 | MS.gene59837:CDS |
| GAACAAAACTCAGTCTCACT+AGG | 0.581117 | 8.3:+54188474 | MS.gene59837:intron |
| TGGGAGACCACAACCTGCAT+CGG | 0.582099 | 8.3:+54189213 | MS.gene59837:CDS |
| CAGTTCCTTAGCCAGAGCAG+CGG | 0.585551 | 8.3:-54189131 | None:intergenic |
| GAAGGCGTCTGAGTTGTACA+TGG | 0.599261 | 8.3:+54189186 | MS.gene59837:CDS |
| TCTGAGTTGTACATGGAGTG+TGG | 0.603285 | 8.3:+54189193 | MS.gene59837:CDS |
| CTGAGTTGTACATGGAGTGT+GGG | 0.611671 | 8.3:+54189194 | MS.gene59837:CDS |
| TTGGGATGCAGCTAAGCACA+TGG | 0.614457 | 8.3:+54189102 | MS.gene59837:intron |
| TAACAACTTGTTGGGATCGG+AGG | 0.615411 | 8.3:-54188166 | None:intergenic |
| AGTTGCAGACTTCTATCAGA+AGG | 0.624852 | 8.3:+54189168 | MS.gene59837:CDS |
| CAAAACTCAGTCTCACTAGG+TGG | 0.687722 | 8.3:+54188477 | MS.gene59837:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATATCAATCAAATTCAA+AGG | - | chr8.3:54188422-54188441 | None:intergenic | 15.0% |
| !!! | TGAACAATAACAATTTTATT+CGG | + | chr8.3:54188741-54188760 | MS.gene59837:intron | 15.0% |
| !! | GATCAAAATCAAAGAATATA+AGG | - | chr8.3:54188969-54188988 | None:intergenic | 20.0% |
| !! | TAAATAGGATTATTGAACTA+TGG | + | chr8.3:54188387-54188406 | MS.gene59837:intron | 20.0% |
| !! | TTGTTCTTATCATGTAATTA+AGG | + | chr8.3:54188349-54188368 | MS.gene59837:intron | 20.0% |
| !!! | AGTATGTCTAAATTACATTT+TGG | + | chr8.3:54188807-54188826 | MS.gene59837:intron | 20.0% |
| !! | ATGTCTAAATTACATTTTGG+CGG | + | chr8.3:54188810-54188829 | MS.gene59837:intron | 25.0% |
| !! | CCTGACAAAACAATTTTATT+CGG | + | chr8.3:54188649-54188668 | MS.gene59837:intron | 25.0% |
| AACATACAGTTTATCAGCTT+TGG | - | chr8.3:54188193-54188212 | None:intergenic | 30.0% | |
| GGTTTCTGTTAAAGTAATAG+CGG | + | chr8.3:54188762-54188781 | MS.gene59837:intron | 30.0% | |
| TGTTCTTGTTCTTTATGAAC+AGG | + | chr8.3:54188523-54188542 | MS.gene59837:CDS | 30.0% | |
| TTCCTATGAATATAATTCGC+AGG | - | chr8.3:54188283-54188302 | None:intergenic | 30.0% | |
| ! | ATGGTTTTCTATGTCGTTAA+TGG | + | chr8.3:54189035-54189054 | MS.gene59837:intron | 30.0% |
| !! | AAAATTGTTTTGTCAGGTAG+CGG | - | chr8.3:54188646-54188665 | None:intergenic | 30.0% |
| !! | CCGAATAAAATTGTTTTGTC+AGG | - | chr8.3:54188652-54188671 | None:intergenic | 30.0% |
| AAAACAATTGCAGTCTCACA+AGG | + | chr8.3:54188698-54188717 | MS.gene59837:intron | 35.0% | |
| CACCTGCGAATTATATTCAT+AGG | + | chr8.3:54188278-54188297 | MS.gene59837:intron | 35.0% | |
| CTATGAATATAATTCGCAGG+TGG | - | chr8.3:54188280-54188299 | None:intergenic | 35.0% | |
| CTGATGAACTCTTCTTATTC+TGG | + | chr8.3:54188850-54188869 | MS.gene59837:intron | 35.0% | |
| ! | AGCTTTCTCATAGGCTATTT+TGG | - | chr8.3:54188916-54188935 | None:intergenic | 35.0% |
| ACAACGTGTACCCTTCATTA+CGG | - | chr8.3:54189014-54189033 | None:intergenic | 40.0% | |
| AGTTGCAGACTTCTATCAGA+AGG | + | chr8.3:54189168-54189187 | MS.gene59837:CDS | 40.0% | |
| CTTGTTCTTTATGAACAGGC+TGG | + | chr8.3:54188527-54188546 | MS.gene59837:CDS | 40.0% | |
| GAACAAAACTCAGTCTCACT+AGG | + | chr8.3:54188474-54188493 | MS.gene59837:intron | 40.0% | |
| TCAGTTTCTGCCGTAATGAA+GGG | + | chr8.3:54189001-54189020 | MS.gene59837:intron | 40.0% | |
| TCCTTGTGAAGCTTTCTCAT+AGG | - | chr8.3:54188925-54188944 | None:intergenic | 40.0% | |
| TCTTATTCTGGTGTAGCGAA+TGG | + | chr8.3:54188862-54188881 | MS.gene59837:intron | 40.0% | |
| TGACAAGTTAACCAGACCTT+GGG | + | chr8.3:54189084-54189103 | MS.gene59837:intron | 40.0% | |
| TTCAGTTTCTGCCGTAATGA+AGG | + | chr8.3:54189000-54189019 | MS.gene59837:intron | 40.0% | |
| TTGACAAGTTAACCAGACCT+TGG | + | chr8.3:54189083-54189102 | MS.gene59837:intron | 40.0% | |
| ! | AGCTTTGGCTAACAACTTGT+TGG | - | chr8.3:54188178-54188197 | None:intergenic | 40.0% |
| ! | GCTTTGGCTAACAACTTGTT+GGG | - | chr8.3:54188177-54188196 | None:intergenic | 40.0% |
| !! | AAGGTCTGGTTAACTTGTCA+AGG | - | chr8.3:54189084-54189103 | None:intergenic | 40.0% |
| !! | GAGACTGCAATTGTTTTGTC+AGG | - | chr8.3:54188695-54188714 | None:intergenic | 40.0% |
| !! | TTACATTTTGGCGGCTATCT+TGG | + | chr8.3:54188819-54188838 | MS.gene59837:intron | 40.0% |
| !!! | TAGGCTATTTTGGTTTCTCG+TGG | - | chr8.3:54188906-54188925 | None:intergenic | 40.0% |
| ATAGCGGCTATCTGACAACA+CGG | + | chr8.3:54188778-54188797 | MS.gene59837:intron | 45.0% | |
| CAAAACTCAGTCTCACTAGG+TGG | + | chr8.3:54188477-54188496 | MS.gene59837:CDS | 45.0% | |
| CTGAGTTGTACATGGAGTGT+GGG | + | chr8.3:54189194-54189213 | MS.gene59837:CDS | 45.0% | |
| GCCTATGAGAAAGCTTCACA+AGG | + | chr8.3:54188921-54188940 | MS.gene59837:intron | 45.0% | |
| TAATTCGCAGGTGGAATCTG+AGG | - | chr8.3:54188271-54188290 | None:intergenic | 45.0% | |
| TCTGAGTTGTACATGGAGTG+TGG | + | chr8.3:54189193-54189212 | MS.gene59837:CDS | 45.0% | |
| TTGCAGATTGCGTGCAATAG+TGG | + | chr8.3:54188608-54188627 | MS.gene59837:intron | 45.0% | |
| ! | ATGAAGGGTACACGTTGTCA+TGG | + | chr8.3:54189016-54189035 | MS.gene59837:intron | 45.0% |
| !! | GGCTAACAACTTGTTGGGAT+CGG | - | chr8.3:54188172-54188191 | None:intergenic | 45.0% |
| !! | TAACAACTTGTTGGGATCGG+AGG | - | chr8.3:54188169-54188188 | None:intergenic | 45.0% |
| !! | TCACTAGGTGGAGTGTTGAT+TGG | + | chr8.3:54188489-54188508 | MS.gene59837:CDS | 45.0% |
| !!! | TTTTGGTTTCTCGTGGTTCC+TGG | - | chr8.3:54188899-54188918 | None:intergenic | 45.0% |
| !!! | TTTTATTATTTATTCTAAAT+AGG | + | chr8.3:54188372-54188391 | MS.gene59837:intron | 5.0% |
| AGAGAGAGAGAGAGAGAGAG+AGG | - | chr8.3:54188219-54188238 | None:intergenic | 50.0% | |
| ATGTGCTTAGCTGCATCCCA+AGG | - | chr8.3:54189103-54189122 | None:intergenic | 50.0% | |
| GCATCGGATGCTCTTGCAAA+GGG | + | chr8.3:54189229-54189248 | MS.gene59837:CDS | 50.0% | |
| TAGCGGCTATCTGACAACAC+GGG | + | chr8.3:54188779-54188798 | MS.gene59837:intron | 50.0% | |
| TGCATCGGATGCTCTTGCAA+AGG | + | chr8.3:54189228-54189247 | MS.gene59837:CDS | 50.0% | |
| TGGCTAAGGAACTGAGCAAC+TGG | + | chr8.3:54189140-54189159 | MS.gene59837:CDS | 50.0% | |
| TTGGGATGCAGCTAAGCACA+TGG | + | chr8.3:54189102-54189121 | MS.gene59837:intron | 50.0% | |
| !! | ATGCTCTTGCAAAGGGTGCT+GGG | + | chr8.3:54189236-54189255 | MS.gene59837:CDS | 50.0% |
| !! | GAAGGCGTCTGAGTTGTACA+TGG | + | chr8.3:54189186-54189205 | MS.gene59837:CDS | 50.0% |
| !! | TTTGCAAGAGCATCCGATGC+AGG | - | chr8.3:54189229-54189248 | None:intergenic | 50.0% |
| CAGTTCCTTAGCCAGAGCAG+CGG | - | chr8.3:54189134-54189153 | None:intergenic | 55.0% | |
| CTTAGCTGCATCCCAAGGTC+TGG | - | chr8.3:54189098-54189117 | None:intergenic | 55.0% | |
| TGGGAGACCACAACCTGCAT+CGG | + | chr8.3:54189213-54189232 | MS.gene59837:CDS | 55.0% | |
| ! | AGAGCATCCGATGCAGGTTG+TGG | - | chr8.3:54189223-54189242 | None:intergenic | 55.0% |
| ! | GATGCTCTTGCAAAGGGTGC+TGG | + | chr8.3:54189235-54189254 | MS.gene59837:CDS | 55.0% |
| !! | CAACTTGTTGGGATCGGAGG+AGG | - | chr8.3:54188166-54188185 | None:intergenic | 55.0% |
| !! | CGAATGGCTTTAGAGCTGCC+AGG | + | chr8.3:54188878-54188897 | MS.gene59837:intron | 55.0% |
| ATCTGCCGCTGCTCTGGCTA+AGG | + | chr8.3:54189126-54189145 | MS.gene59837:CDS | 60.0% | |
| CATGGAATCTGCCGCTGCTC+TGG | + | chr8.3:54189120-54189139 | MS.gene59837:CDS | 60.0% | |
| CGGCTATCTGACAACACGGG+TGG | + | chr8.3:54188782-54188801 | MS.gene59837:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 54188159 | 54189261 | 54188159 | ID=MS.gene59837 |
| chr8.3 | mRNA | 54188159 | 54189261 | 54188159 | ID=MS.gene59837.t1;Parent=MS.gene59837 |
| chr8.3 | exon | 54188159 | 54188205 | 54188159 | ID=MS.gene59837.t1.exon1;Parent=MS.gene59837.t1 |
| chr8.3 | CDS | 54188159 | 54188205 | 54188159 | ID=cds.MS.gene59837.t1;Parent=MS.gene59837.t1 |
| chr8.3 | exon | 54188475 | 54188548 | 54188475 | ID=MS.gene59837.t1.exon2;Parent=MS.gene59837.t1 |
| chr8.3 | CDS | 54188475 | 54188548 | 54188475 | ID=cds.MS.gene59837.t1;Parent=MS.gene59837.t1 |
| chr8.3 | exon | 54189113 | 54189261 | 54189113 | ID=MS.gene59837.t1.exon3;Parent=MS.gene59837.t1 |
| chr8.3 | CDS | 54189113 | 54189261 | 54189113 | ID=cds.MS.gene59837.t1;Parent=MS.gene59837.t1 |
| Gene Sequence |
| Protein sequence |
