Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59850.t1 | XP_013445244.1 | 92.8 | 152 | 11 | 0 | 1 | 152 | 1 | 152 | 7.10E-79 | 303.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59850.t1 | Q9SSK5 | 35.3 | 150 | 94 | 3 | 3 | 150 | 7 | 155 | 1.1e-22 | 107.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59850.t1 | A0A072U0G0 | 92.8 | 152 | 11 | 0 | 1 | 152 | 1 | 152 | 5.1e-79 | 303.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05240 | MS.gene59850 | 0.802848 | 4.62E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene59850 | 0.802543 | 5.34E-49 | -1.69E-46 |
| MS.gene056697 | MS.gene59850 | 0.810283 | 1.24E-50 | -1.69E-46 |
| MS.gene058381 | MS.gene59850 | 0.918374 | 1.69E-86 | -1.69E-46 |
| MS.gene060087 | MS.gene59850 | 0.806572 | 7.71E-50 | -1.69E-46 |
| MS.gene060358 | MS.gene59850 | 0.827248 | 1.74E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59850.t1 | MTR_8g045555 | 92.763 | 152 | 11 | 0 | 1 | 152 | 1 | 152 | 2.62e-103 | 292 |
| MS.gene59850.t1 | MTR_8g045520 | 76.159 | 151 | 36 | 0 | 1 | 151 | 1 | 151 | 5.37e-87 | 251 |
| MS.gene59850.t1 | MTR_8g045490 | 75.000 | 152 | 38 | 0 | 1 | 152 | 1 | 152 | 5.99e-84 | 243 |
| MS.gene59850.t1 | MTR_8g045570 | 72.368 | 152 | 42 | 0 | 1 | 152 | 1 | 152 | 4.34e-83 | 241 |
| MS.gene59850.t1 | MTR_8g045400 | 73.026 | 152 | 40 | 1 | 1 | 152 | 1 | 151 | 4.55e-81 | 236 |
| MS.gene59850.t1 | MTR_8g045695 | 69.079 | 152 | 46 | 1 | 1 | 152 | 1 | 151 | 3.41e-77 | 226 |
| MS.gene59850.t1 | MTR_8g045300 | 66.887 | 151 | 50 | 0 | 1 | 151 | 1 | 151 | 5.53e-77 | 226 |
| MS.gene59850.t1 | MTR_8g045735 | 67.105 | 152 | 49 | 1 | 1 | 152 | 1 | 151 | 1.60e-74 | 219 |
| MS.gene59850.t1 | MTR_8g045665 | 66.447 | 152 | 50 | 1 | 1 | 152 | 1 | 151 | 2.12e-73 | 217 |
| MS.gene59850.t1 | MTR_8g045640 | 66.447 | 152 | 50 | 1 | 1 | 152 | 1 | 151 | 1.16e-72 | 215 |
| MS.gene59850.t1 | MTR_8g045560 | 65.132 | 152 | 52 | 1 | 1 | 152 | 1 | 151 | 1.50e-70 | 209 |
| MS.gene59850.t1 | MTR_1g030810 | 43.137 | 153 | 84 | 2 | 1 | 151 | 1 | 152 | 2.23e-41 | 135 |
| MS.gene59850.t1 | MTR_1g030820 | 37.255 | 153 | 93 | 2 | 1 | 151 | 1 | 152 | 1.38e-37 | 126 |
| MS.gene59850.t1 | MTR_1g030840 | 39.103 | 156 | 87 | 4 | 1 | 149 | 1 | 155 | 3.62e-32 | 112 |
| MS.gene59850.t1 | MTR_1g031640 | 36.486 | 148 | 85 | 4 | 7 | 152 | 7 | 147 | 5.49e-29 | 103 |
| MS.gene59850.t1 | MTR_8g012550 | 34.483 | 145 | 92 | 3 | 6 | 149 | 8 | 150 | 2.75e-27 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59850.t1 | AT1G70890 | 35.333 | 150 | 94 | 3 | 3 | 150 | 7 | 155 | 6.68e-28 | 101 |
| MS.gene59850.t1 | AT1G70830 | 35.333 | 150 | 94 | 3 | 3 | 150 | 22 | 170 | 5.28e-27 | 99.8 |
| MS.gene59850.t1 | AT1G70840 | 33.333 | 150 | 97 | 3 | 3 | 150 | 20 | 168 | 5.29e-27 | 99.8 |
| MS.gene59850.t1 | AT5G28010 | 34.000 | 150 | 96 | 3 | 3 | 150 | 15 | 163 | 6.03e-27 | 99.4 |
| MS.gene59850.t1 | AT5G28010 | 34.000 | 150 | 96 | 3 | 3 | 150 | 15 | 163 | 6.03e-27 | 99.4 |
| MS.gene59850.t1 | AT1G70830 | 35.333 | 150 | 94 | 3 | 3 | 150 | 22 | 170 | 1.36e-26 | 99.4 |
| MS.gene59850.t1 | AT1G70830 | 35.333 | 150 | 94 | 3 | 3 | 150 | 22 | 170 | 4.53e-26 | 99.4 |
| MS.gene59850.t1 | AT1G70830 | 35.333 | 150 | 94 | 3 | 3 | 150 | 22 | 170 | 1.73e-25 | 99.4 |
| MS.gene59850.t1 | AT1G70830 | 34.000 | 150 | 96 | 3 | 3 | 150 | 184 | 332 | 8.03e-24 | 95.1 |
| MS.gene59850.t1 | AT1G70850 | 33.333 | 150 | 97 | 3 | 3 | 150 | 9 | 157 | 1.63e-24 | 95.1 |
| MS.gene59850.t1 | AT1G70830 | 34.000 | 150 | 96 | 3 | 3 | 150 | 137 | 285 | 2.63e-24 | 95.5 |
| MS.gene59850.t1 | AT1G70830 | 32.258 | 93 | 62 | 1 | 59 | 150 | 31 | 123 | 4.74e-14 | 67.8 |
| MS.gene59850.t1 | AT1G70850 | 33.333 | 150 | 97 | 3 | 3 | 150 | 9 | 157 | 5.28e-24 | 95.1 |
| MS.gene59850.t1 | AT1G70850 | 34.286 | 140 | 89 | 3 | 13 | 150 | 175 | 313 | 1.59e-23 | 94.0 |
| MS.gene59850.t1 | AT1G70850 | 33.333 | 150 | 97 | 3 | 3 | 150 | 9 | 157 | 5.28e-24 | 95.1 |
| MS.gene59850.t1 | AT1G70850 | 34.286 | 140 | 89 | 3 | 13 | 150 | 175 | 313 | 1.59e-23 | 94.0 |
| MS.gene59850.t1 | AT1G23130 | 30.201 | 149 | 102 | 2 | 3 | 150 | 10 | 157 | 7.20e-23 | 89.0 |
| MS.gene59850.t1 | AT1G70870 | 35.762 | 151 | 84 | 4 | 1 | 151 | 1 | 138 | 1.46e-22 | 87.4 |
| MS.gene59850.t1 | AT5G28000 | 29.333 | 150 | 103 | 3 | 3 | 150 | 13 | 161 | 1.18e-21 | 85.9 |
| MS.gene59850.t1 | AT1G70880 | 28.667 | 150 | 104 | 3 | 3 | 150 | 8 | 156 | 9.53e-21 | 83.2 |
| MS.gene59850.t1 | AT1G23120 | 27.778 | 144 | 99 | 3 | 7 | 150 | 7 | 145 | 1.82e-20 | 82.4 |
| MS.gene59850.t1 | AT1G23120 | 27.778 | 144 | 99 | 3 | 7 | 150 | 57 | 195 | 7.33e-20 | 82.0 |
| MS.gene59850.t1 | AT1G14930 | 31.818 | 154 | 100 | 4 | 1 | 152 | 1 | 151 | 1.20e-19 | 80.5 |
| MS.gene59850.t1 | AT4G23670 | 32.026 | 153 | 101 | 3 | 1 | 152 | 1 | 151 | 8.35e-19 | 78.2 |
| MS.gene59850.t1 | AT2G01520 | 30.065 | 153 | 104 | 3 | 1 | 152 | 1 | 151 | 7.57e-18 | 75.5 |
| MS.gene59850.t1 | AT1G24020 | 30.263 | 152 | 104 | 2 | 1 | 151 | 1 | 151 | 3.04e-16 | 71.6 |
| MS.gene59850.t1 | AT1G24020 | 30.263 | 152 | 104 | 2 | 1 | 151 | 1 | 151 | 3.04e-16 | 71.6 |
| MS.gene59850.t1 | AT4G14060 | 30.065 | 153 | 104 | 3 | 1 | 152 | 1 | 151 | 3.84e-16 | 71.2 |
| MS.gene59850.t1 | AT1G14940 | 29.139 | 151 | 104 | 3 | 1 | 150 | 1 | 149 | 9.40e-16 | 70.1 |
| MS.gene59850.t1 | AT4G23680 | 29.412 | 153 | 105 | 3 | 1 | 152 | 1 | 151 | 1.04e-15 | 70.1 |
| MS.gene59850.t1 | AT1G35260 | 23.973 | 146 | 107 | 3 | 7 | 150 | 6 | 149 | 1.70e-15 | 69.3 |
| MS.gene59850.t1 | AT2G01530 | 28.758 | 153 | 106 | 3 | 1 | 152 | 1 | 151 | 1.97e-15 | 69.3 |
| MS.gene59850.t1 | AT1G14950 | 30.065 | 153 | 102 | 4 | 1 | 151 | 1 | 150 | 1.29e-14 | 67.4 |
| MS.gene59850.t1 | AT1G30990 | 27.152 | 151 | 106 | 4 | 1 | 150 | 1 | 148 | 5.63e-13 | 62.8 |
| MS.gene59850.t1 | AT1G14960 | 26.974 | 152 | 108 | 3 | 1 | 151 | 1 | 150 | 8.94e-12 | 59.7 |
| MS.gene59850.t1 | AT3G26450 | 25.974 | 154 | 110 | 4 | 1 | 152 | 1 | 152 | 1.60e-11 | 58.9 |
Find 27 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCAAGGATGGTGCTTAA+TGG | 0.283777 | 8.3:+53821040 | None:intergenic |
| AAGCTCTTTGGTGGAGATAT+TGG | 0.286213 | 8.3:+53831248 | MS.gene59850:CDS |
| AAACTCACTTACAAGCTCTT+TGG | 0.349937 | 8.3:+53831236 | MS.gene59850:CDS |
| AAACATTGGACTTATGTCAT+AGG | 0.403651 | 8.3:+53821214 | MS.gene59850:CDS |
| ATATTCAATAGTCCATTTGA+TGG | 0.406324 | 8.3:-53831333 | None:intergenic |
| TAGAGATATTGATGCTCATC+TGG | 0.421031 | 8.3:+53831418 | MS.gene59850:CDS |
| ATTCAAATAATTGATAATAG+TGG | 0.432059 | 8.3:+53831299 | MS.gene59850:CDS |
| ATCATCACCTTCATGTAGCT+TGG | 0.443216 | 8.3:-53821170 | None:intergenic |
| ACGTAGTCCATCCAGCCATT+AGG | 0.444330 | 8.3:-53831383 | None:intergenic |
| AGATATTGGTGAGCACTATA+AGG | 0.448051 | 8.3:+53831262 | MS.gene59850:CDS |
| GCAGCAGTGCTGCCATCAAA+TGG | 0.452968 | 8.3:+53831321 | MS.gene59850:CDS |
| GAGGATATTGACCCTCCTAA+TGG | 0.460289 | 8.3:+53831368 | MS.gene59850:CDS |
| GGTAAACTTAGTACTGAACT+TGG | 0.476618 | 8.3:+53821061 | MS.gene59850:CDS |
| CTCACTTACAAGCTCTTTGG+TGG | 0.490559 | 8.3:+53831239 | MS.gene59850:CDS |
| AGCTACATGAAGGTGATGAT+TGG | 0.504052 | 8.3:+53821173 | MS.gene59850:CDS |
| ATATTGACCCTCCTAATGGC+TGG | 0.525673 | 8.3:+53831372 | MS.gene59850:CDS |
| TTTCATGAACTCTTTCACAA+TGG | 0.533960 | 8.3:-53821147 | None:intergenic |
| TAGTCCATCCAGCCATTAGG+AGG | 0.553714 | 8.3:-53831380 | None:intergenic |
| TGACCCTCCTAATGGCTGGA+TGG | 0.577019 | 8.3:+53831376 | MS.gene59850:CDS |
| GTAAACTTAGTACTGAACTT+GGG | 0.605653 | 8.3:+53821062 | MS.gene59850:CDS |
| ACACTGATACGGTTAAACAT+TGG | 0.605669 | 8.3:+53821200 | MS.gene59850:CDS |
| CATGAAACCAAGCTACATGA+AGG | 0.607386 | 8.3:+53821163 | MS.gene59850:CDS |
| TGATTGGCATCACACTGATA+CGG | 0.619683 | 8.3:+53821189 | MS.gene59850:CDS |
| TCACATGACAACAGATGGCA+AGG | 0.632897 | 8.3:+53831166 | MS.gene59850:intron |
| GAAATTGTAGAACTTGTCAA+CGG | 0.635731 | 8.3:-53821095 | None:intergenic |
| AGTCCATCCAGCCATTAGGA+GGG | 0.659406 | 8.3:-53831379 | None:intergenic |
| TGAATATGAGAAGATCAATG+AGG | 0.667184 | 8.3:+53831349 | MS.gene59850:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 53821049 | 53831451 | 53821049 | ID=MS.gene59850 |
| chr8.3 | mRNA | 53821049 | 53831451 | 53821049 | ID=MS.gene59850.t1;Parent=MS.gene59850 |
| chr8.3 | exon | 53821049 | 53821235 | 53821049 | ID=MS.gene59850.t1.exon1;Parent=MS.gene59850.t1 |
| chr8.3 | CDS | 53821049 | 53821235 | 53821049 | ID=cds.MS.gene59850.t1;Parent=MS.gene59850.t1 |
| chr8.3 | exon | 53831180 | 53831451 | 53831180 | ID=MS.gene59850.t1.exon2;Parent=MS.gene59850.t1 |
| chr8.3 | CDS | 53831180 | 53831451 | 53831180 | ID=cds.MS.gene59850.t1;Parent=MS.gene59850.t1 |
| Gene Sequence |
| Protein sequence |
