AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60224

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60224.t1 MTR_4g009040 91.459 281 24 0 3 283 45 325 0.0 526
MS.gene60224.t1 MTR_4g009040 90.175 285 24 1 3 283 45 329 0.0 520
MS.gene60224.t1 MTR_4g009690 91.844 282 23 0 3 284 45 326 0.0 506
MS.gene60224.t1 MTR_4g009690 91.489 282 23 1 3 284 45 325 1.81e-180 500
MS.gene60224.t1 MTR_4g006940 42.349 281 153 3 5 281 51 326 2.03e-65 208
MS.gene60224.t1 MTR_2g104960 43.060 281 151 3 5 281 54 329 6.24e-64 204
MS.gene60224.t1 MTR_5g072620 39.427 279 162 2 8 281 43 319 3.63e-62 199
MS.gene60224.t1 MTR_4g006940 44.872 234 124 2 5 238 51 279 3.31e-58 187
MS.gene60224.t1 MTR_4g006940 44.872 234 124 2 5 238 51 279 3.31e-58 187
MS.gene60224.t1 MTR_2g028620 39.502 281 161 4 9 282 49 327 4.53e-57 186
MS.gene60224.t1 MTR_3g005170 37.410 278 165 6 6 277 46 320 2.15e-52 174
MS.gene60224.t1 MTR_3g031650 36.823 277 172 1 8 281 43 319 3.13e-52 173
MS.gene60224.t1 MTR_4g077100 37.943 282 164 7 3 277 44 321 3.14e-52 174
MS.gene60224.t1 MTR_2g101330 35.587 281 178 3 6 283 41 321 5.10e-52 173
MS.gene60224.t1 MTR_2g028620 43.421 228 127 2 9 234 49 276 8.49e-52 172
MS.gene60224.t1 MTR_3g005210 37.770 278 164 6 6 277 42 316 9.16e-51 170
MS.gene60224.t1 MTR_8g062440 39.085 284 151 9 6 277 44 317 1.74e-49 166
MS.gene60224.t1 MTR_8g062440 39.085 284 151 9 6 277 45 318 2.08e-49 166
MS.gene60224.t1 MTR_4g077130 38.380 284 154 8 6 277 45 319 2.84e-49 166
MS.gene60224.t1 MTR_8g062110 37.979 287 156 8 3 277 42 318 4.16e-49 166
MS.gene60224.t1 MTR_8g062110 37.979 287 156 8 3 277 51 327 4.78e-49 166
MS.gene60224.t1 MTR_5g056600 32.862 283 180 4 6 281 40 319 6.43e-47 159
MS.gene60224.t1 MTR_4g077100 38.655 238 140 5 3 237 44 278 3.42e-46 157
MS.gene60224.t1 MTR_3g005170 37.872 235 140 5 6 237 46 277 1.35e-45 155
MS.gene60224.t1 MTR_3g031650 37.004 227 143 0 8 234 43 269 2.42e-45 154
MS.gene60224.t1 MTR_1g022440 33.916 286 175 10 6 281 44 325 3.43e-43 150
MS.gene60224.t1 MTR_8g062110 37.302 252 144 7 3 251 42 282 6.82e-43 148
MS.gene60224.t1 MTR_8g062440 38.889 252 140 8 6 254 44 284 2.21e-42 148
MS.gene60224.t1 MTR_1g022445 33.217 286 177 9 6 281 44 325 3.80e-42 147
MS.gene60224.t1 MTR_8g062110 37.931 232 137 5 3 231 42 269 4.74e-42 146
MS.gene60224.t1 MTR_3g005170 33.094 278 146 6 6 277 46 289 6.37e-40 140
MS.gene60224.t1 MTR_4g092080 33.110 299 175 10 1 280 45 337 4.85e-36 132
MS.gene60224.t1 MTR_4g081440 31.596 307 183 10 1 283 48 351 3.65e-33 124
MS.gene60224.t1 MTR_4g081440 31.148 305 188 9 1 283 52 356 3.98e-32 122
MS.gene60224.t1 MTR_8g062110 35.065 231 129 7 59 277 11 232 5.94e-32 118
MS.gene60224.t1 MTR_8g062710 85.246 61 9 0 3 63 42 102 2.91e-30 110
MS.gene60224.t1 MTR_7g074880 30.249 281 184 6 10 281 49 326 8.32e-30 115
MS.gene60224.t1 MTR_7g074820 31.208 298 184 9 1 281 36 329 1.30e-29 114
MS.gene60224.t1 MTR_7g074730 29.392 296 191 8 1 281 36 328 2.97e-28 110
MS.gene60224.t1 MTR_8g062708 82.540 63 11 0 176 238 5 67 4.81e-28 104
MS.gene60224.t1 MTR_7g074870 30.249 281 184 7 10 281 11 288 1.06e-26 105
MS.gene60224.t1 MTR_5g029990 32.184 261 140 8 18 261 56 296 1.21e-26 105
MS.gene60224.t1 MTR_7g074710 30.820 305 173 13 1 281 36 326 5.79e-26 104
MS.gene60224.t1 MTR_7g074850 29.225 284 186 7 10 281 49 329 2.59e-25 103
MS.gene60224.t1 MTR_8g062110 34.091 176 110 4 59 231 11 183 3.91e-25 99.4
MS.gene60224.t1 MTR_4g023730 32.313 294 165 11 9 281 49 329 1.61e-23 98.2
MS.gene60224.t1 MTR_6g406250 30.180 222 146 4 11 226 95 313 1.86e-20 90.1
MS.gene60224.t1 MTR_0164s0070 48.315 89 45 1 6 93 41 129 3.20e-19 84.0
MS.gene60224.t1 MTR_0685s0030 33.129 163 95 4 123 277 2 158 8.21e-17 76.3
MS.gene60224.t1 MTR_2g013230 26.899 316 182 14 1 281 39 340 6.32e-16 77.0
MS.gene60224.t1 MTR_6g406250 31.788 151 99 2 11 157 95 245 8.78e-16 75.9
MS.gene60224.t1 MTR_2g013250 23.794 311 194 11 1 279 38 337 1.63e-12 67.0
MS.gene60224.t1 MTR_7g117190 25.296 253 149 10 1 223 38 280 1.67e-11 63.9
MS.gene60224.t1 MTR_7g117190 25.296 253 149 10 1 223 38 280 1.76e-11 63.9
MS.gene60224.t1 MTR_3g011130 33.333 117 73 4 62 174 30 145 3.08e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60224.t1 AT2G33590 62.816 277 102 1 6 281 44 320 2.99e-116 337
MS.gene60224.t1 AT2G33600 61.011 277 107 1 6 281 44 320 9.64e-107 313
MS.gene60224.t1 AT1G76470 50.178 281 135 2 6 281 42 322 6.52e-83 252
MS.gene60224.t1 AT1G80820 40.283 283 160 3 3 281 40 317 6.55e-61 196
MS.gene60224.t1 AT1G51410 41.549 284 155 7 6 282 45 324 6.49e-60 193
MS.gene60224.t1 AT5G58490 38.989 277 166 1 8 281 47 323 1.59e-58 190
MS.gene60224.t1 AT1G09510 43.262 282 151 6 6 281 44 322 1.89e-58 189
MS.gene60224.t1 AT2G02400 36.691 278 169 2 9 281 43 318 2.64e-57 186
MS.gene60224.t1 AT1G15950 39.858 281 160 3 5 281 47 322 1.04e-56 186
MS.gene60224.t1 AT1G15950 39.858 281 160 3 5 281 47 322 1.04e-56 186
MS.gene60224.t1 AT5G19440 39.576 283 162 6 6 282 46 325 2.24e-56 184
MS.gene60224.t1 AT1G80820 43.220 236 129 2 3 238 40 270 1.17e-53 176
MS.gene60224.t1 AT1G15950 39.483 271 155 3 5 271 47 312 1.91e-53 177
MS.gene60224.t1 AT1G15950 42.678 239 132 2 5 243 47 280 8.25e-53 174
MS.gene60224.t1 AT1G15950 43.220 236 129 2 5 240 47 277 1.05e-52 174
MS.gene60224.t1 AT1G15950 43.162 234 128 2 5 238 47 275 1.17e-51 171
MS.gene60224.t1 AT1G09490 36.655 281 171 5 6 281 13 291 1.47e-50 168
MS.gene60224.t1 AT1G09490 36.655 281 171 5 6 281 44 322 4.88e-50 168
MS.gene60224.t1 AT1G09480 37.722 281 168 5 6 281 44 322 2.37e-49 166
MS.gene60224.t1 AT1G66800 40.780 282 154 7 6 281 44 318 4.09e-49 165
MS.gene60224.t1 AT1G09480 37.722 281 168 5 6 281 91 369 5.65e-49 166
MS.gene60224.t1 AT1G09500 37.456 283 169 5 6 281 10 291 2.08e-47 160
MS.gene60224.t1 AT1G09500 37.456 283 169 5 6 281 44 325 9.17e-47 159
MS.gene60224.t1 AT4G35420 33.798 287 174 6 6 283 44 323 1.98e-46 159
MS.gene60224.t1 AT1G09500 41.818 220 125 3 6 223 44 262 8.87e-45 153
MS.gene60224.t1 AT1G15950 38.393 224 134 1 62 281 1 224 5.89e-42 145
MS.gene60224.t1 AT1G68540 31.119 286 191 3 2 281 36 321 3.41e-39 139
MS.gene60224.t1 AT5G42800 34.266 286 174 9 6 281 44 325 1.20e-38 139
MS.gene60224.t1 AT1G25460 31.915 282 185 4 6 281 39 319 1.17e-37 135
MS.gene60224.t1 AT2G45400 31.741 293 171 10 9 281 24 307 8.55e-31 117
MS.gene60224.t1 AT2G45400 31.741 293 171 10 9 281 81 364 2.91e-30 117
MS.gene60224.t1 AT2G23910 32.599 227 137 4 1 222 7 222 3.28e-30 115
MS.gene60224.t1 AT2G23910 32.727 220 132 4 8 222 48 256 8.52e-30 114
MS.gene60224.t1 AT2G23910 32.727 220 132 4 8 222 48 256 9.55e-30 114
MS.gene60224.t1 AT2G23910 32.727 220 132 4 8 222 48 256 1.37e-29 114
MS.gene60224.t1 AT1G61720 31.457 302 174 12 6 284 49 340 1.72e-29 114
MS.gene60224.t1 AT4G27250 31.944 288 174 9 1 270 41 324 1.82e-29 114
MS.gene60224.t1 AT4G27250 31.973 294 176 9 1 270 41 334 1.65e-28 112
MS.gene60224.t1 AT4G30470 33.014 209 125 4 18 222 58 255 3.94e-28 110
MS.gene60224.t1 AT1G68540 30.435 207 140 2 79 281 8 214 6.45e-26 102
MS.gene60224.t1 AT2G45400 32.510 243 138 8 9 237 81 311 9.83e-25 101
MS.gene60224.t1 AT5G14700 35.149 202 120 6 32 226 120 317 1.76e-20 90.5
MS.gene60224.t1 AT2G45400 35.227 176 100 5 9 174 81 252 9.80e-19 84.0
MS.gene60224.t1 AT5G14700 34.483 145 91 3 32 172 120 264 7.18e-15 73.6

Find 46 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTGTCTTACTCTTGTTT+TGG 0.178833 4.3:+85830473 MS.gene60224:CDS
TCATCAATCGCTTTATCTTT+TGG 0.244785 4.3:-85830155 None:intergenic
CTCAGAGAAACTGCGAAGTT+TGG 0.267800 4.3:+85831830 MS.gene60224:CDS
TTTAGCAAGTGTTCATATTT+CGG 0.276272 4.3:-85829768 None:intergenic
GAAGTTTGGGTTGGAAATTC+AGG 0.291234 4.3:+85831844 MS.gene60224:CDS
CGTAGTTGATGCAATACTTT+TGG 0.300274 4.3:+85831136 MS.gene60224:CDS
AATCTTTAAAGCAGATATCT+TGG 0.328019 4.3:+85829818 MS.gene60224:CDS
ATATGAACACTTGCTAAAAC+TGG 0.332196 4.3:+85829773 MS.gene60224:CDS
CTGTTCTTCTGCTTCTGTCT+TGG 0.343397 4.3:-85830409 None:intergenic
CACTATCAACACACGAGATT+TGG 0.344680 4.3:+85831205 MS.gene60224:CDS
TATCTGCTTTAAAGATTGTA+AGG 0.370846 4.3:-85829812 None:intergenic
CAAGACAGAAGCAGAAGAAC+AGG 0.371007 4.3:+85830410 MS.gene60224:CDS
AACCAAGCTACTTGCATTTG+TGG 0.373321 4.3:-85830514 None:intergenic
TTTGTGGTTGACTGTAAAAT+TGG 0.374021 4.3:-85830498 None:intergenic
AGAAACATTACCTCAGGATT+AGG 0.383683 4.3:-85829921 None:intergenic
CTGACTTTCGCGAAAAGAAC+TGG 0.387817 4.3:+85830435 MS.gene60224:CDS
TCAGTTTACTCTGCAATTGT+TGG 0.395610 4.3:+85829849 MS.gene60224:CDS
TCAGAGAAACTGCGAAGTTT+GGG 0.401403 4.3:+85831831 MS.gene60224:CDS
GTTAGAAGGCTGCGATTCAT+TGG 0.405647 4.3:+85831079 MS.gene60224:intron
TGACTCTGTTGAGAGCTATA+AGG 0.425631 4.3:+85831887 MS.gene60224:CDS
GAAAATGAAGCTTACTTCGT+AGG 0.425779 4.3:-85831266 None:intergenic
CATTGGAGAATAAGCTTCGT+TGG 0.446781 4.3:+85831096 MS.gene60224:CDS
AAGCGATTGATGAGTCTTGT+TGG 0.452091 4.3:+85830165 MS.gene60224:CDS
GTCTGCCTTGTGATTCTCAT+AGG 0.459100 4.3:-85831159 None:intergenic
TTAGCAGTTCCCTTCACTGC+AGG 0.496393 4.3:-85830047 None:intergenic
GGTACGACTGTTGAAGGTAC+AGG 0.501620 4.3:-85829900 None:intergenic
GGATTAGGTACGACTGTTGA+AGG 0.504659 4.3:-85829906 None:intergenic
AAAATGAAGCTTACTTCGTA+GGG 0.514933 4.3:-85831265 None:intergenic
TATCAACACACGAGATTTGG+TGG 0.518864 4.3:+85831208 MS.gene60224:CDS
GGGTTGGAAATTCAGGCCAT+TGG 0.519755 4.3:+85831851 MS.gene60224:CDS
GTTGAGAGCTATAAGGAAGC+TGG 0.520202 4.3:+85831894 MS.gene60224:CDS
TTGGAAATTCAGGCCATTGG+AGG 0.526899 4.3:+85831854 MS.gene60224:CDS
AGAAACTGCGAAGTTTGGGT+TGG 0.531335 4.3:+85831835 MS.gene60224:CDS
TCAATGAGTGTTTCCTCCAA+TGG 0.543548 4.3:-85831867 None:intergenic
AACCACAAATGCAAGTAGCT+TGG 0.550767 4.3:+85830512 MS.gene60224:CDS
ATGAGAATCACAAGGCAGAC+GGG 0.568344 4.3:+85831162 MS.gene60224:CDS
ATGATTGAGCCTGCAGTGAA+GGG 0.570026 4.3:+85830038 MS.gene60224:CDS
AATGATTGAGCCTGCAGTGA+AGG 0.570953 4.3:+85830037 MS.gene60224:CDS
TATGAGAATCACAAGGCAGA+CGG 0.581333 4.3:+85831161 MS.gene60224:CDS
AAGGTACAGGGCTAGCAACA+TGG 0.584108 4.3:-85829887 None:intergenic
TGCTGGCAGCTATATTGAGA+TGG 0.610431 4.3:+85831788 MS.gene60224:intron
GTACGACTGTTGAAGGTACA+GGG 0.633213 4.3:-85829899 None:intergenic
AGCTTGTCTCAAAGCTAATG+TGG 0.639403 4.3:+85830079 MS.gene60224:CDS
GAAAAGAACTGGACTTAACG+TGG 0.670351 4.3:+85830446 MS.gene60224:CDS
AACAGTCGTACCTAATCCTG+AGG 0.679893 4.3:+85829911 MS.gene60224:CDS
TTTGGCCTATGAGAATCACA+AGG 0.727463 4.3:+85831154 MS.gene60224:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTGATAATATTTAAATTTCA+AGG - chr4.3:85831314-85831333 None:intergenic 15.0%
!! TAATATTTAAATTTCAAGGT+TGG - chr4.3:85831310-85831329 None:intergenic 15.0%
!! TGAAATTAGTTAAATTATAC+TGG - chr4.3:85831000-85831019 None:intergenic 15.0%
!!! ATTTTGAAGAAATTTTTGAT+TGG + chr4.3:85829464-85829483 MS.gene60224:intron 15.0%
!! AAGTCATTAATTAATTAACG+AGG + chr4.3:85831403-85831422 MS.gene60224:intron 20.0%
!! ACTTCTTAGATATATTTACA+GGG - chr4.3:85829542-85829561 None:intergenic 20.0%
!!! AAAGAAAGAATTTTTTGCAA+CGG + chr4.3:85831370-85831389 MS.gene60224:intron 20.0%
!!! ATTTGATAAAACAGAAGTAT+TGG - chr4.3:85831654-85831673 None:intergenic 20.0%
!!! CTGTTTTATCAAATTATCAT+TGG + chr4.3:85831660-85831679 MS.gene60224:intron 20.0%
!!! CTTTATCTTTTGGTAAATTA+GGG - chr4.3:85830148-85830167 None:intergenic 20.0%
!!! TTCAATCATCAAAGAATTTT+GGG + chr4.3:85830334-85830353 MS.gene60224:intron 20.0%
!!! TTTCAATCATCAAAGAATTT+TGG + chr4.3:85830333-85830352 MS.gene60224:intron 20.0%
! CACTTCTTAGATATATTTAC+AGG - chr4.3:85829543-85829562 None:intergenic 25.0%
! CATAACTTCTATAAAATCCT+TGG + chr4.3:85830660-85830679 MS.gene60224:intron 25.0%
! GGTTGAAAACAAAACATAAA+AGG - chr4.3:85829393-85829412 None:intergenic 25.0%
! GTTGTTATAAGAACTTGATA+AGG - chr4.3:85830574-85830593 None:intergenic 25.0%
! TGTAACCATCAAATCTATAT+AGG - chr4.3:85831747-85831766 None:intergenic 25.0%
! TTAACGAGGAATATATGTAA+AGG + chr4.3:85831417-85831436 MS.gene60224:intron 25.0%
! TTATGTGGTTATCATTATCA+TGG - chr4.3:85830645-85830664 None:intergenic 25.0%
! TTGTGATCAACAATTGTATA+AGG + chr4.3:85831462-85831481 MS.gene60224:intron 25.0%
! TTTAGCAAGTGTTCATATTT+CGG - chr4.3:85829771-85829790 None:intergenic 25.0%
! TTTGTATACAATTGTTGAAG+AGG + chr4.3:85829656-85829675 MS.gene60224:intron 25.0%
!! AGGATTTTATAGAAGTTATG+TGG - chr4.3:85830660-85830679 None:intergenic 25.0%
!! GCTTTATCTTTTGGTAAATT+AGG - chr4.3:85830149-85830168 None:intergenic 25.0%
!! TATGTTAAAGTTTTCATTGC+TGG + chr4.3:85831771-85831790 MS.gene60224:intron 25.0%
!! TTTTGGCGTCAATATTTATT+TGG + chr4.3:85830731-85830750 MS.gene60224:intron 25.0%
!!! AATCTTTAAAGCAGATATCT+TGG + chr4.3:85829818-85829837 MS.gene60224:CDS 25.0%
!!! ATTTGTCTTACTCTTGTTTT+GGG + chr4.3:85830474-85830493 MS.gene60224:CDS 25.0%
!!! CGAAGCAAAGAATTTTTTTT+TGG + chr4.3:85829586-85829605 MS.gene60224:intron 25.0%
!!! TATCTGCTTTAAAGATTGTA+AGG - chr4.3:85829815-85829834 None:intergenic 25.0%
!!! TTTTTGAAAAGGTCATATAC+AGG - chr4.3:85829426-85829445 None:intergenic 25.0%
AAAATGAAGCTTACTTCGTA+GGG - chr4.3:85831268-85831287 None:intergenic 30.0%
AACATTAGAAACATTACCTC+AGG - chr4.3:85829930-85829949 None:intergenic 30.0%
AAGTGTCCAATAAGTGTTTA+AGG + chr4.3:85829558-85829577 MS.gene60224:intron 30.0%
AAGTTATGCAGAGAATAATC+CGG - chr4.3:85830969-85830988 None:intergenic 30.0%
ACTTGCCTATATAGATTTGA+TGG + chr4.3:85831739-85831758 MS.gene60224:intron 30.0%
AGACTACTGCAAAAATACTA+AGG + chr4.3:85830196-85830215 MS.gene60224:CDS 30.0%
CATTATCATGGAAAACAACA+AGG - chr4.3:85830633-85830652 None:intergenic 30.0%
GACACTACTATCATATCATA+AGG - chr4.3:85830269-85830288 None:intergenic 30.0%
GACTTACCTTAAACACTTAT+TGG - chr4.3:85829567-85829586 None:intergenic 30.0%
GTATGGACATACACATATAA+AGG - chr4.3:85830843-85830862 None:intergenic 30.0%
TCAAAAGAATGAGAGAATAG+AGG - chr4.3:85829693-85829712 None:intergenic 30.0%
TTTGTGGTTGACTGTAAAAT+TGG - chr4.3:85830501-85830520 None:intergenic 30.0%
! ATATGAACACTTGCTAAAAC+TGG + chr4.3:85829773-85829792 MS.gene60224:CDS 30.0%
! GTTCTCGTCAAACTTTTAAA+AGG + chr4.3:85830534-85830553 MS.gene60224:CDS 30.0%
! TAGCAGATAGTTTAGCTTAA+CGG - chr4.3:85830914-85830933 None:intergenic 30.0%
! TCATCAATCGCTTTATCTTT+TGG - chr4.3:85830158-85830177 None:intergenic 30.0%
! TCTATTCTCTCATTCTTTTG+AGG + chr4.3:85829692-85829711 MS.gene60224:intron 30.0%
!! CATTTGTCTTACTCTTGTTT+TGG + chr4.3:85830473-85830492 MS.gene60224:CDS 30.0%
AAAAGGTCATATACAGGTCA+TGG - chr4.3:85829420-85829439 None:intergenic 35.0%
AACACTTGTCGTATGAATGT+TGG + chr4.3:85829609-85829628 MS.gene60224:intron 35.0%
ACTTGATAAGGCATATCAGT+AGG - chr4.3:85830562-85830581 None:intergenic 35.0%
AGAAACATTACCTCAGGATT+AGG - chr4.3:85829924-85829943 None:intergenic 35.0%
AGTTATGCAGAGAATAATCC+GGG - chr4.3:85830968-85830987 None:intergenic 35.0%
ATCATCCTGTCTTTGTTAGA+AGG + chr4.3:85831065-85831084 MS.gene60224:intron 35.0%
ATTGTCGCATCGAAAAATTC+AGG + chr4.3:85831573-85831592 MS.gene60224:intron 35.0%
CAAATGAATCGCAAAAACAC+TGG - chr4.3:85829720-85829739 None:intergenic 35.0%
GAAAATGAAGCTTACTTCGT+AGG - chr4.3:85831269-85831288 None:intergenic 35.0%
TCAGTTTACTCTGCAATTGT+TGG + chr4.3:85829849-85829868 MS.gene60224:CDS 35.0%
TCATATACAGGTCATGGAAT+TGG - chr4.3:85829414-85829433 None:intergenic 35.0%
TCATGAATTACGAATATGCC+CGG + chr4.3:85830947-85830966 MS.gene60224:intron 35.0%
! GCATAACAGAATCAGAGTTT+TGG + chr4.3:85830714-85830733 MS.gene60224:intron 35.0%
!! CATCGAAAAATTCAGGTTGA+AGG + chr4.3:85831580-85831599 MS.gene60224:intron 35.0%
!! CGTAGTTGATGCAATACTTT+TGG + chr4.3:85831136-85831155 MS.gene60224:CDS 35.0%
!! TGCTGAGCAGATTTTTGAAA+AGG - chr4.3:85829437-85829456 None:intergenic 35.0%
AAAACACACCAGTAATGTGC+AGG + chr4.3:85830010-85830029 MS.gene60224:intron 40.0%
AAATAGTCTCCTACATGCGT+TGG - chr4.3:85829638-85829657 None:intergenic 40.0%
AACCAAGCTACTTGCATTTG+TGG - chr4.3:85830517-85830536 None:intergenic 40.0%
AACCACAAATGCAAGTAGCT+TGG + chr4.3:85830512-85830531 MS.gene60224:CDS 40.0%
AGCTTGTCTCAAAGCTAATG+TGG + chr4.3:85830079-85830098 MS.gene60224:CDS 40.0%
CACTATCAACACACGAGATT+TGG + chr4.3:85831205-85831224 MS.gene60224:CDS 40.0%
CATTGGAGAATAAGCTTCGT+TGG + chr4.3:85831096-85831115 MS.gene60224:CDS 40.0%
GAAAAGAACTGGACTTAACG+TGG + chr4.3:85830446-85830465 MS.gene60224:CDS 40.0%
TAATTTGATATGTCACCGCG+TGG + chr4.3:85829965-85829984 MS.gene60224:intron 40.0%
TATCAACACACGAGATTTGG+TGG + chr4.3:85831208-85831227 MS.gene60224:CDS 40.0%
TATGAGAATCACAAGGCAGA+CGG + chr4.3:85831161-85831180 MS.gene60224:CDS 40.0%
TCAATGAGTGTTTCCTCCAA+TGG - chr4.3:85831870-85831889 None:intergenic 40.0%
TCAGAGAAACTGCGAAGTTT+GGG + chr4.3:85831831-85831850 MS.gene60224:CDS 40.0%
TGACTCTGTTGAGAGCTATA+AGG + chr4.3:85831887-85831906 MS.gene60224:CDS 40.0%
TGTCAGTATCGTCTTAATGC+CGG - chr4.3:85831618-85831637 None:intergenic 40.0%
TTGCAAGTAATGTTGAGCGA+CGG + chr4.3:85831520-85831539 MS.gene60224:intron 40.0%
TTTGGCCTATGAGAATCACA+AGG + chr4.3:85831154-85831173 MS.gene60224:CDS 40.0%
! AAGCGATTGATGAGTCTTGT+TGG + chr4.3:85830165-85830184 MS.gene60224:CDS 40.0%
! AGTTCTTTTCGCGAAAGTCA+GGG - chr4.3:85830436-85830455 None:intergenic 40.0%
! CTTCACCGCTATAGTGTTTT+GGG + chr4.3:85830882-85830901 MS.gene60224:intron 40.0%
! TTTTTCCCTGCTCATTCTCA+TGG - chr4.3:85831500-85831519 None:intergenic 40.0%
!! GAAGTTTGGGTTGGAAATTC+AGG + chr4.3:85831844-85831863 MS.gene60224:CDS 40.0%
!!! TGGTTCTAGTTTTGCCTTCA+TGG + chr4.3:85829985-85830004 MS.gene60224:intron 40.0%
AAACACTATAGCGGTGAAGC+AGG - chr4.3:85830881-85830900 None:intergenic 45.0%
AACAGTCGTACCTAATCCTG+AGG + chr4.3:85829911-85829930 MS.gene60224:CDS 45.0%
AAGGCAAAACTAGAACCACG+CGG - chr4.3:85829983-85830002 None:intergenic 45.0%
AATGATTGAGCCTGCAGTGA+AGG + chr4.3:85830037-85830056 MS.gene60224:CDS 45.0%
AGAAACTGCGAAGTTTGGGT+TGG + chr4.3:85831835-85831854 MS.gene60224:CDS 45.0%
ATGAGAATCACAAGGCAGAC+GGG + chr4.3:85831162-85831181 MS.gene60224:CDS 45.0%
ATGATTGAGCCTGCAGTGAA+GGG + chr4.3:85830038-85830057 MS.gene60224:CDS 45.0%
CAAGACAGAAGCAGAAGAAC+AGG + chr4.3:85830410-85830429 MS.gene60224:CDS 45.0%
CATTTCCACCTGCACATTAC+TGG - chr4.3:85830021-85830040 None:intergenic 45.0%
CGACTCCCAAAACACTATAG+CGG - chr4.3:85830890-85830909 None:intergenic 45.0%
CTCAGAGAAACTGCGAAGTT+TGG + chr4.3:85831830-85831849 MS.gene60224:CDS 45.0%
CTGACTTTCGCGAAAAGAAC+TGG + chr4.3:85830435-85830454 MS.gene60224:CDS 45.0%
CTGTTCTTCTGCTTCTGTCT+TGG - chr4.3:85830412-85830431 None:intergenic 45.0%
GGATTAGGTACGACTGTTGA+AGG - chr4.3:85829909-85829928 None:intergenic 45.0%
GTCTGCCTTGTGATTCTCAT+AGG - chr4.3:85831162-85831181 None:intergenic 45.0%
GTTAGAAGGCTGCGATTCAT+TGG + chr4.3:85831079-85831098 MS.gene60224:intron 45.0%
GTTGAGAGCTATAAGGAAGC+TGG + chr4.3:85831894-85831913 MS.gene60224:CDS 45.0%
TAGAGCCATGAGAATGAGCA+GGG + chr4.3:85831492-85831511 MS.gene60224:intron 45.0%
TGCTGGCAGCTATATTGAGA+TGG + chr4.3:85831788-85831807 MS.gene60224:intron 45.0%
TGGCATAACAACACCAGTTC+TGG - chr4.3:85830392-85830411 None:intergenic 45.0%
TTGGAAATTCAGGCCATTGG+AGG + chr4.3:85831854-85831873 MS.gene60224:CDS 45.0%
! CAGTTCTTTTCGCGAAAGTC+AGG - chr4.3:85830437-85830456 None:intergenic 45.0%
! CTGGTGTGTTTTCACCATGA+AGG - chr4.3:85830002-85830021 None:intergenic 45.0%
! GCTTCACCGCTATAGTGTTT+TGG + chr4.3:85830881-85830900 MS.gene60224:intron 45.0%
! GGTAGTGGTAGTATGGTGTA+TGG - chr4.3:85830860-85830879 None:intergenic 45.0%
! GTACGACTGTTGAAGGTACA+GGG - chr4.3:85829902-85829921 None:intergenic 45.0%
! TGAAGCAGGTAGTGGTAGTA+TGG - chr4.3:85830867-85830886 None:intergenic 45.0%
! TTGAAGGTGTGTCTTGTGTC+CGG + chr4.3:85831596-85831615 MS.gene60224:intron 45.0%
!!! CGCTATAGTGTTTTGGGAGT+CGG + chr4.3:85830888-85830907 MS.gene60224:intron 45.0%
ACACACCAGTAATGTGCAGG+TGG + chr4.3:85830013-85830032 MS.gene60224:intron 50.0%
ACGCTACTGTTACACAGCCA+AGG - chr4.3:85830680-85830699 None:intergenic 50.0%
CGCAGCCTTCTAACAAAGAC+AGG - chr4.3:85831073-85831092 None:intergenic 50.0%
CTTCGCATGTGATCCAGAAC+TGG + chr4.3:85830376-85830395 MS.gene60224:intron 50.0%
GGGTTGGAAATTCAGGCCAT+TGG + chr4.3:85831851-85831870 MS.gene60224:CDS 50.0%
GTAGAGCCATGAGAATGAGC+AGG + chr4.3:85831491-85831510 MS.gene60224:intron 50.0%
GTCAGTGTCAGTGTCGTGTT+CGG + chr4.3:85831692-85831711 MS.gene60224:intron 50.0%
TATAGCGGTGAAGCAGGTAG+TGG - chr4.3:85830875-85830894 None:intergenic 50.0%
TGTTGGACACCAACGCATGT+AGG + chr4.3:85829626-85829645 MS.gene60224:intron 50.0%
TTAGCAGTTCCCTTCACTGC+AGG - chr4.3:85830050-85830069 None:intergenic 50.0%
TTCGGACTACCTGCAGTTGT+CGG - chr4.3:85829753-85829772 None:intergenic 50.0%
! AAGGTACAGGGCTAGCAACA+TGG - chr4.3:85829890-85829909 None:intergenic 50.0%
! GGTACGACTGTTGAAGGTAC+AGG - chr4.3:85829903-85829922 None:intergenic 50.0%
AGTGCATGTCCGACAACTGC+AGG + chr4.3:85829741-85829760 MS.gene60224:intron 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 85829376 85831935 85829376 ID=MS.gene60224
chr4.3 mRNA 85829376 85831935 85829376 ID=MS.gene60224.t1;Parent=MS.gene60224
chr4.3 exon 85829376 85829382 85829376 ID=MS.gene60224.t1.exon1;Parent=MS.gene60224.t1
chr4.3 CDS 85829376 85829382 85829376 ID=cds.MS.gene60224.t1;Parent=MS.gene60224.t1
chr4.3 exon 85829763 85829932 85829763 ID=MS.gene60224.t1.exon2;Parent=MS.gene60224.t1
chr4.3 CDS 85829763 85829932 85829763 ID=cds.MS.gene60224.t1;Parent=MS.gene60224.t1
chr4.3 exon 85830032 85830217 85830032 ID=MS.gene60224.t1.exon3;Parent=MS.gene60224.t1
chr4.3 CDS 85830032 85830217 85830032 ID=cds.MS.gene60224.t1;Parent=MS.gene60224.t1
chr4.3 exon 85830393 85830555 85830393 ID=MS.gene60224.t1.exon4;Parent=MS.gene60224.t1
chr4.3 CDS 85830393 85830555 85830393 ID=cds.MS.gene60224.t1;Parent=MS.gene60224.t1
chr4.3 exon 85831084 85831273 85831084 ID=MS.gene60224.t1.exon5;Parent=MS.gene60224.t1
chr4.3 CDS 85831084 85831273 85831084 ID=cds.MS.gene60224.t1;Parent=MS.gene60224.t1
chr4.3 exon 85831797 85831935 85831797 ID=MS.gene60224.t1.exon6;Parent=MS.gene60224.t1
chr4.3 CDS 85831797 85831935 85831797 ID=cds.MS.gene60224.t1;Parent=MS.gene60224.t1
Gene Sequence

>MS.gene60224

ATGAGAAGTGAGAACCTTTTATGTTTTGTTTTCAACCAATTCCATGACCTGTATATGACCTTTTCAAAAATCTGCTCAGCAGTTGAAAATTTTGAAGAAATTTTTGATTGGTTAATAGCATTTCAAATAGTCATATGATTGTTGAAGCAAATAAATTTTAAATCCCTGTAAATATATCTAAGAAGTGTCCAATAAGTGTTTAAGGTAAGTCGAAGCAAAGAATTTTTTTTTGGAACACTTGTCGTATGAATGTTGGACACCAACGCATGTAGGAGACTATTTTGTATACAATTGTTGAAGAGGATGTTGTTATGCCTCTATTCTCTCATTCTTTTGAGGTACCAGTGTTTTTGCGATTCATTTGAAGTGCATGTCCGACAACTGCAGGTAGTCCGAAATATGAACACTTGCTAAAACTGGAGAAAGCTTTTGAGAACCTTACAATCTTTAAAGCAGATATCTTGGATTACGTATCAGTTTACTCTGCAATTGTTGGATGCAGTGCAGTATTCCATGTTGCTAGCCCTGTACCTTCAACAGTCGTACCTAATCCTGAGGTAATGTTTCTAATGTTCTTTTATATGCAGACTAATTTGATATGTCACCGCGTGGTTCTAGTTTTGCCTTCATGGTGAAAACACACCAGTAATGTGCAGGTGGAAATGATTGAGCCTGCAGTGAAGGGAACTGCTAATGTACTTGAAGCTTGTCTCAAAGCTAATGTGGAACGTGTCGTCTTTGTATCATCTGTAGCTGCTTTTGCTATCAACCCTAATTTACCAAAAGATAAAGCGATTGATGAGTCTTGTTGGTCTGACAAAGACTACTGCAAAAATACTAAGGTCACTAAGATTTTATTATATTTGTGCATGACATAAATGTATCATTAGCCTTATGATATGATAGTAGTGTCTTATAATTTTTTTTCTTCAGATCTTTAAGTTTCAAAATGTTATGTTTCAATCATCAAAGAATTTTGGGTTATGTAAATAAGCTTATACTTCGCATGTGATCCAGAACTGGTGTTGTTATGCCAAGACAGAAGCAGAAGAACAGGCCCTGACTTTCGCGAAAAGAACTGGACTTAACGTGGTAAGCATTTGTCTTACTCTTGTTTTGGGACCAATTTTACAGTCAACCACAAATGCAAGTAGCTTGGTTCTCGTCAAACTTTTAAAAGGTACCTACTGATATGCCTTATCAAGTTCTTATAACAACATGCATATTTGTGAACTGTATAATCGAGTTAAAATTCCTTGTTGTTTTCCATGATAATGATAACCACATAACTTCTATAAAATCCTTGGCTGTGTAACAGTAGCGTTTGATTTTTAGTGTGCATAACAGAATCAGAGTTTTGGCGTCAATATTTATTTGGATATTATGTTTGAATATTAGTAGCAATTTTTTTTGATGATTAGTTATAAATATCTGTTAAGTTTTGTGTGTTCTTCTATTTTTTAGCCTTTATATGTGTATGTCCATACACCATACTACCACTACCTGCTTCACCGCTATAGTGTTTTGGGAGTCGGCCGTTAAGCTAAACTATCTGCTACTAAATAGATAGATCATGAATTACGAATATGCCCGGATTATTCTCTGCATAACTTGTTTTCTGCCAGTATAATTTAACTAATTTCATATACTATAGATTTCATATAAATGCTAAAATCATTTTTCATGTTAATCATCCTGTCTTTGTTAGAAGGCTGCGATTCATTGGAGAATAAGCTTCGTTGGATTGTTGACGTACAAGACGTAGTTGATGCAATACTTTTGGCCTATGAGAATCACAAGGCAGACGGGAGATACATCTGCACTTCACACACTATCAACACACGAGATTTGGTGGAGAAATCGAAGAGTATATATCTGAACTACAAGTACCCTACGAAGTAAGCTTCATTTTCTAAAGTAGTAGTCATAAACCAACCTTGAAATTTAAATATTATCAGTAATATTAATAATATCAAGATATATAAGAAATGTGAAAAGAAAGAATTTTTTGCAACGGAAAACTAAAGAAGTCATTAATTAATTAACGAGGAATATATGTAAAGGAGATTAACTATTACGCATGTTCTTGTGATCAACAATTGTATAAGGAAAATTGTAGAGCCATGAGAATGAGCAGGGAAAAATTGCAAGTAATGTTGAGCGACGGAGTACTTGCAGTGTCAATCTTAATAGTTGCATTGTCGCATCGAAAAATTCAGGTTGAAGGTGTGTCTTGTGTCCGGCATTAAGACGATACTGACACATGTGTTTAAATCCAATACTTCTGTTTTATCAAATTATCATTGGTGTTAGCGTGTCAGTGTCAGTGTCGTGTTCGGTGTATGTGTTAGTGTTTCATAGCTACTTGCCTATATAGATTTGATGGTTACAATCTTATGTTAAAGTTTTCATTGCTGGCAGCTATATTGAGATGGATGATTACAAAATGCTGAGCTCAGAGAAACTGCGAAGTTTGGGTTGGAAATTCAGGCCATTGGAGGAAACACTCATTGACTCTGTTGAGAGCTATAAGGAAGCTGGACTACTTCAATCAGAATAA
Protein sequence

>MS.gene60224.t1

MRSSPKYEHLLKLEKAFENLTIFKADILDYVSVYSAIVGCSAVFHVASPVPSTVVPNPEVEMIEPAVKGTANVLEACLKANVERVVFVSSVAAFAINPNLPKDKAIDESCWSDKDYCKNTKNWCCYAKTEAEEQALTFAKRTGLNVVSICLTLVLGPILQSTTNASSLVLVKLLKEGCDSLENKLRWIVDVQDVVDAILLAYENHKADGRYICTSHTINTRDLVEKSKSIYLNYKYPTNYIEMDDYKMLSSEKLRSLGWKFRPLEETLIDSVESYKEAGLLQSE