Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60230.t1 | XP_024638013.1 | 94.3 | 106 | 6 | 0 | 1 | 106 | 1 | 106 | 7.70E-48 | 199.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60230.t1 | Q9SSC1 | 41.5 | 142 | 43 | 6 | 1 | 106 | 1 | 138 | 1.9e-15 | 83.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60230.t1 | A0A396HYZ6 | 94.3 | 106 | 6 | 0 | 1 | 106 | 1 | 106 | 5.6e-48 | 199.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene36231 | MS.gene60230 | PPI |
| MS.gene89049 | MS.gene60230 | PPI |
| MS.gene36229 | MS.gene60230 | PPI |
| MS.gene60230 | MS.gene86369 | PPI |
| MS.gene060879 | MS.gene60230 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60230.t1 | MTR_4g011060 | 94.286 | 105 | 6 | 0 | 1 | 105 | 1 | 105 | 6.93e-69 | 202 |
| MS.gene60230.t1 | MTR_2g103380 | 57.627 | 118 | 38 | 1 | 1 | 106 | 1 | 118 | 5.43e-39 | 126 |
| MS.gene60230.t1 | MTR_4g114050 | 52.128 | 94 | 33 | 4 | 1 | 93 | 1 | 83 | 4.01e-23 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60230.t1 | AT1G80180 | 41.549 | 142 | 43 | 6 | 1 | 106 | 1 | 138 | 2.33e-23 | 87.8 |
| MS.gene60230.t1 | AT5G20100 | 48.980 | 98 | 46 | 2 | 3 | 96 | 2 | 99 | 3.83e-22 | 84.0 |
| MS.gene60230.t1 | AT1G15400 | 40.152 | 132 | 45 | 6 | 1 | 103 | 1 | 127 | 1.82e-19 | 77.8 |
| MS.gene60230.t1 | AT1G15400 | 40.152 | 132 | 45 | 6 | 1 | 103 | 1 | 127 | 1.88e-19 | 77.8 |
| MS.gene60230.t1 | AT1G15400 | 40.152 | 132 | 45 | 6 | 1 | 103 | 1 | 127 | 2.16e-19 | 77.8 |
Find 29 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGTTCATCTGGTCTTGTT+TGG | 0.224185 | 4.3:+85775994 | MS.gene60230:CDS |
| AGGTTCATCTGGTCTTGTTT+GGG | 0.315565 | 4.3:+85775995 | MS.gene60230:CDS |
| TGTGGATTCTGTAGCCCTTT+TGG | 0.323362 | 4.3:+85776206 | MS.gene60230:CDS |
| CTACCTCGACCGACGTTTAC+CGG | 0.339840 | 4.3:-85776252 | None:intergenic |
| TGGGATGACAGATTCTTATC+AGG | 0.348639 | 4.3:+85776014 | MS.gene60230:CDS |
| CTGAATGATACTTCAGATCT+TGG | 0.351403 | 4.3:-85775966 | None:intergenic |
| GGAGACGGTGGTTCGATCGC+TGG | 0.391781 | 4.3:-85776170 | None:intergenic |
| TTCAGAAGATCAGGTTCATC+TGG | 0.432719 | 4.3:+85775984 | MS.gene60230:CDS |
| GAAGTATCATTCAGAAGATC+AGG | 0.445781 | 4.3:+85775975 | MS.gene60230:CDS |
| AGAAGCCATTCCACCGGTGC+TGG | 0.447002 | 4.3:+85776119 | MS.gene60230:CDS |
| GGTGGAATGGCTTCTTTGTA+CGG | 0.453050 | 4.3:-85776111 | None:intergenic |
| GAGACGGTGGTTCGATCGCT+GGG | 0.462717 | 4.3:-85776169 | None:intergenic |
| CTCGTCCAGCACCGGTGGAA+TGG | 0.498060 | 4.3:-85776124 | None:intergenic |
| CATTCCACCGGTGCTGGACG+AGG | 0.514080 | 4.3:+85776125 | MS.gene60230:CDS |
| AATCCACACGCAGAAATCTT+CGG | 0.515261 | 4.3:-85776191 | None:intergenic |
| TACCTCGACCGACGTTTACC+GGG | 0.526089 | 4.3:-85776251 | None:intergenic |
| ACGGTATCCTCGTCCAGCAC+CGG | 0.568784 | 4.3:-85776132 | None:intergenic |
| AGCCCGGTAAACGTCGGTCG+AGG | 0.570758 | 4.3:+85776249 | MS.gene60230:CDS |
| AAGGGTCAACGATCAAAGCC+CGG | 0.598386 | 4.3:+85776233 | MS.gene60230:CDS |
| TCTCCGAAGATTTCTGCGTG+TGG | 0.607577 | 4.3:+85776188 | MS.gene60230:CDS |
| CGGTAAACGTCGGTCGAGGT+AGG | 0.609038 | 4.3:+85776253 | MS.gene60230:CDS |
| GGAATGGCTTCTTTGTACGG+TGG | 0.613872 | 4.3:-85776108 | None:intergenic |
| GTATCCTCGTCCAGCACCGG+TGG | 0.623334 | 4.3:-85776129 | None:intergenic |
| CACGCAGAAATCTTCGGAGA+CGG | 0.628142 | 4.3:-85776185 | None:intergenic |
| GCAGAAATCTTCGGAGACGG+TGG | 0.631582 | 4.3:-85776182 | None:intergenic |
| GCTGGACGAGGATACCGTAC+CGG | 0.652103 | 4.3:+85776137 | MS.gene60230:CDS |
| AGACGGTGGTTCGATCGCTG+GGG | 0.689604 | 4.3:-85776168 | None:intergenic |
| GTACAAAGAAGCCATTCCAC+CGG | 0.692582 | 4.3:+85776113 | MS.gene60230:CDS |
| GATCAAAGCCCGGTAAACGT+CGG | 0.721418 | 4.3:+85776243 | MS.gene60230:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTGAATGATACTTCAGATCT+TGG | - | chr4.3:85775969-85775988 | None:intergenic | 35.0% | |
| ! | GAAGTATCATTCAGAAGATC+AGG | + | chr4.3:85775975-85775994 | MS.gene60230:CDS | 35.0% |
| !! | TGTAGCCCTTTTGGAAAAAA+GGG | + | chr4.3:85776215-85776234 | MS.gene60230:CDS | 35.0% |
| !!! | GTTGACCCTTTTTTCCAAAA+GGG | - | chr4.3:85776223-85776242 | None:intergenic | 35.0% |
| AATCCACACGCAGAAATCTT+CGG | - | chr4.3:85776194-85776213 | None:intergenic | 40.0% | |
| TGGGATGACAGATTCTTATC+AGG | + | chr4.3:85776014-85776033 | MS.gene60230:CDS | 40.0% | |
| TTCAGAAGATCAGGTTCATC+TGG | + | chr4.3:85775984-85776003 | MS.gene60230:CDS | 40.0% | |
| ! | GGTTTTCACATGAAGCTCTT+TGG | - | chr4.3:85776090-85776109 | None:intergenic | 40.0% |
| !! | AGGTTCATCTGGTCTTGTTT+GGG | + | chr4.3:85775995-85776014 | MS.gene60230:CDS | 40.0% |
| !! | CTGTAGCCCTTTTGGAAAAA+AGG | + | chr4.3:85776214-85776233 | MS.gene60230:CDS | 40.0% |
| !!! | CGTTGACCCTTTTTTCCAAA+AGG | - | chr4.3:85776224-85776243 | None:intergenic | 40.0% |
| GGTGGAATGGCTTCTTTGTA+CGG | - | chr4.3:85776114-85776133 | None:intergenic | 45.0% | |
| GTACAAAGAAGCCATTCCAC+CGG | + | chr4.3:85776113-85776132 | MS.gene60230:CDS | 45.0% | |
| ! | TGTGGATTCTGTAGCCCTTT+TGG | + | chr4.3:85776206-85776225 | MS.gene60230:CDS | 45.0% |
| !! | CAGGTTCATCTGGTCTTGTT+TGG | + | chr4.3:85775994-85776013 | MS.gene60230:CDS | 45.0% |
| AAGGGTCAACGATCAAAGCC+CGG | + | chr4.3:85776233-85776252 | MS.gene60230:CDS | 50.0% | |
| CACGCAGAAATCTTCGGAGA+CGG | - | chr4.3:85776188-85776207 | None:intergenic | 50.0% | |
| GATCAAAGCCCGGTAAACGT+CGG | + | chr4.3:85776243-85776262 | MS.gene60230:CDS | 50.0% | |
| GGAATGGCTTCTTTGTACGG+TGG | - | chr4.3:85776111-85776130 | None:intergenic | 50.0% | |
| TCTCCGAAGATTTCTGCGTG+TGG | + | chr4.3:85776188-85776207 | MS.gene60230:CDS | 50.0% | |
| CTACCTCGACCGACGTTTAC+CGG | - | chr4.3:85776255-85776274 | None:intergenic | 55.0% | |
| GCAGAAATCTTCGGAGACGG+TGG | - | chr4.3:85776185-85776204 | None:intergenic | 55.0% | |
| TACCTCGACCGACGTTTACC+GGG | - | chr4.3:85776254-85776273 | None:intergenic | 55.0% | |
| ! | CTGGGGAGACTTTTCCGGTA+CGG | - | chr4.3:85776154-85776173 | None:intergenic | 55.0% |
| ! | GATCGCTGGGGAGACTTTTC+CGG | - | chr4.3:85776159-85776178 | None:intergenic | 55.0% |
| ACGGTATCCTCGTCCAGCAC+CGG | - | chr4.3:85776135-85776154 | None:intergenic | 60.0% | |
| AGAAGCCATTCCACCGGTGC+TGG | + | chr4.3:85776119-85776138 | MS.gene60230:CDS | 60.0% | |
| AGACGGTGGTTCGATCGCTG+GGG | - | chr4.3:85776171-85776190 | None:intergenic | 60.0% | |
| GAGACGGTGGTTCGATCGCT+GGG | - | chr4.3:85776172-85776191 | None:intergenic | 60.0% | |
| GCTGGACGAGGATACCGTAC+CGG | + | chr4.3:85776137-85776156 | MS.gene60230:CDS | 60.0% | |
| AGCCCGGTAAACGTCGGTCG+AGG | + | chr4.3:85776249-85776268 | MS.gene60230:CDS | 65.0% | |
| GGAGACGGTGGTTCGATCGC+TGG | - | chr4.3:85776173-85776192 | None:intergenic | 65.0% | |
| ! | CATTCCACCGGTGCTGGACG+AGG | + | chr4.3:85776125-85776144 | MS.gene60230:CDS | 65.0% |
| ! | GTATCCTCGTCCAGCACCGG+TGG | - | chr4.3:85776132-85776151 | None:intergenic | 65.0% |
| !! | CTCGTCCAGCACCGGTGGAA+TGG | - | chr4.3:85776127-85776146 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 85775954 | 85776274 | 85775954 | ID=MS.gene60230 |
| chr4.3 | mRNA | 85775954 | 85776274 | 85775954 | ID=MS.gene60230.t1;Parent=MS.gene60230 |
| chr4.3 | exon | 85775954 | 85776274 | 85775954 | ID=MS.gene60230.t1.exon1;Parent=MS.gene60230.t1 |
| chr4.3 | CDS | 85775954 | 85776274 | 85775954 | ID=cds.MS.gene60230.t1;Parent=MS.gene60230.t1 |
| Gene Sequence |
| Protein sequence |
