AlfalfaGEDB | MS.gene60695

M. sativa cultivar XinJiangDaYe / MS.gene60695

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60695.t1	XP_003592080.1	95.1	184	9	0	1	184	1	184	2.00E-88	335.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60695.t1	G7IBX7	95.1	184	9	0	1	184	1	184	1.5e-88	335.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052818	MS.gene60695	-0.810525	1.10E-50	-1.69E-46
MS.gene056091	MS.gene60695	0.809529	1.81E-50	-1.69E-46
MS.gene056403	MS.gene60695	0.824953	6.10E-54	-1.69E-46
MS.gene056605	MS.gene60695	0.824799	6.64E-54	-1.69E-46
MS.gene057079	MS.gene60695	0.808756	2.65E-50	-1.69E-46
MS.gene058238	MS.gene60695	0.802097	6.59E-49	-1.69E-46
MS.gene06001	MS.gene60695	0.800056	1.72E-48	-1.69E-46
MS.gene06011	MS.gene60695	0.829908	3.96E-55	-1.69E-46
MS.gene060586	MS.gene60695	0.821646	3.61E-53	-1.69E-46
MS.gene060830	MS.gene60695	0.855463	6.20E-62	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60695.t1	MTR_1g098510	95.109	184	9	0	1	184	1	184	4.89e-129	360

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60695.t1	AT5G09270	56.842	95	36	2	90	184	19	108	1.65e-31	110
MS.gene60695.t1	AT5G09270	56.842	95	36	2	90	184	19	108	1.65e-31	110

Find 52 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCCTCCTGCTGCAGGCTTTA+TGG	0.092653	1.2:-70881059	MS.gene60695:CDS
CTCTTCTGCTACGGGCTTTA+TGG	0.095185	1.2:-70881230	MS.gene60695:CDS
TATATGGCGGATTTGGTCTT+TGG	0.234560	1.2:-70880958	MS.gene60695:CDS
ATTCTTGTATATGGCGGATT+TGG	0.303440	1.2:-70880965	MS.gene60695:CDS
ATAGATATAGAAAGCTTCTT+AGG	0.309898	1.2:-70880928	MS.gene60695:CDS
GGTTCCTATATTCTTGTATA+TGG	0.337081	1.2:-70880974	MS.gene60695:CDS
TTCTGCCAAAGTGGTTGAAA+AGG	0.353945	1.2:-70880819	MS.gene60695:CDS
CTTGGTCCTCACCAGTTTCC+AGG	0.356142	1.2:+70881092	None:intergenic
TGCAGGCTTTATGGATTCAT+TGG	0.369416	1.2:-70881050	MS.gene60695:CDS
CAGAAACTTATCCTTATGTA+TGG	0.372733	1.2:-70880997	MS.gene60695:CDS
TGGATAAGTGCTTCAGAACT+TGG	0.383586	1.2:+70881283	None:intergenic
TTATGGATTCATTGGAAAAT+CGG	0.384322	1.2:-70881042	MS.gene60695:CDS
TGATTTGCCATGTCCTTGGC+TGG	0.408407	1.2:+70881160	None:intergenic
AAGAAGCTTTCTATATCTAT+AGG	0.413891	1.2:+70880931	None:intergenic
GTCGGTGCCCCTCCTGCTGC+AGG	0.419080	1.2:-70881067	MS.gene60695:CDS
CAGGAGGGGCACCGACTACT+TGG	0.421600	1.2:+70881074	None:intergenic
AAGTTCTGCAGCACCTATCC+TGG	0.422828	1.2:-70881110	MS.gene60695:CDS
AGGATAGGTGCTGCAGAACT+TGG	0.446859	1.2:+70881112	None:intergenic
AGTCGGTGGCTCTTCTGCTA+CGG	0.454743	1.2:-70881239	MS.gene60695:CDS
GCAGCACCTATCCTGGAAAC+TGG	0.465240	1.2:-70881103	MS.gene60695:CDS
GAAGCACTTATCCAGAAAAC+TGG	0.468163	1.2:-70881274	MS.gene60695:CDS
GTCGGTGGCTCTTCTGCTAC+GGG	0.474768	1.2:-70881238	MS.gene60695:CDS
TCCGCCATATACAAGAATAT+AGG	0.486205	1.2:+70880970	None:intergenic
TGGTTGATTTGCCATGTCCT+TGG	0.486730	1.2:+70881156	None:intergenic
ACTACGTGAGATGGTTGAAA+AGG	0.493284	1.2:-70880873	MS.gene60695:CDS
AGCTTCTTAGGACAGAGGAC+AGG	0.494425	1.2:-70880916	MS.gene60695:CDS
AGAAACTTATCCTTATGTAT+GGG	0.501079	1.2:-70880996	MS.gene60695:CDS
TCTGCCAAAGTGGTTGAAAA+GGG	0.502981	1.2:-70880818	MS.gene60695:CDS
ATATAGGAACCCCATACATA+AGG	0.508413	1.2:+70880986	None:intergenic
TCCTATATTCTTGTATATGG+CGG	0.519420	1.2:-70880971	MS.gene60695:CDS
TTCTAGTAATTCTGCCAAAG+TGG	0.522258	1.2:-70880828	MS.gene60695:CDS
ATGTTGCAATGTAGGCTACT+TGG	0.523428	1.2:+70880772	None:intergenic
CATAAAGCCTGCAGCAGGAG+GGG	0.525453	1.2:+70881060	None:intergenic
TCCTCACCAGTTTCCAGGAT+AGG	0.526596	1.2:+70881097	None:intergenic
TCCATAAAGCCTGCAGCAGG+AGG	0.530529	1.2:+70881058	None:intergenic
TTAGGACAGAGGACAGGGTA+CGG	0.537011	1.2:-70880910	MS.gene60695:CDS
GAATCCATAAAGCCTGCAGC+AGG	0.543830	1.2:+70881055	None:intergenic
TAGGACAGAGGACAGGGTAC+GGG	0.558112	1.2:-70880909	MS.gene60695:CDS
GGAAGGAATCTCAGAAGATG+TGG	0.565631	1.2:+70880793	None:intergenic
GAAACTTATCCTTATGTATG+GGG	0.570070	1.2:-70880995	MS.gene60695:CDS
TCAAGCAAGACTACGTGAGA+TGG	0.592566	1.2:-70880882	MS.gene60695:CDS
CCATAAAGCCTGCAGCAGGA+GGG	0.618716	1.2:+70881059	None:intergenic
TAGCTGGAACACTCCAGCCA+AGG	0.621030	1.2:-70881173	MS.gene60695:CDS
GAACACTCCAGCCAAGGACA+TGG	0.636957	1.2:-70881167	MS.gene60695:CDS
GCTTCTTAGGACAGAGGACA+GGG	0.639564	1.2:-70880915	MS.gene60695:CDS
ACCTATCCTGGAAACTGGTG+AGG	0.641675	1.2:-70881098	MS.gene60695:CDS
TGCAATGTAGGCTACTTGGA+AGG	0.646398	1.2:+70880776	None:intergenic
ACTGGCGAGGATCAAGTAGT+CGG	0.654403	1.2:-70881256	MS.gene60695:CDS
TAGAAAGCTTCTTAGGACAG+AGG	0.663471	1.2:-70880921	MS.gene60695:CDS
ACTGGTGAGGACCAAGTAGT+CGG	0.664340	1.2:-70881085	MS.gene60695:CDS
GGCGAGGATCAAGTAGTCGG+TGG	0.665719	1.2:-70881253	MS.gene60695:CDS
ACTTATCCAGAAAACTGGCG+AGG	0.696112	1.2:-70881269	MS.gene60695:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTATGGATTCATTGAAAAAT+CGG	-	chr1.2:70880893-70880912	MS.gene60695:CDS	20.0%
!	AAGAAGCTTTCTATATCTAT+AGG	+	chr1.2:70881178-70881197	None:intergenic	25.0%
!	AGAAACTTATCCTTATGTAT+GGG	-	chr1.2:70881110-70881129	MS.gene60695:CDS	25.0%
!	ATAGATATAGAAAGCTTCTT+AGG	-	chr1.2:70881178-70881197	MS.gene60695:CDS	25.0%
!	ATCAAAAAATCATAGATAGC+TGG	-	chr1.2:70880917-70880936	MS.gene60695:CDS	25.0%
!	TTATGGATTCATTGGAAAAT+CGG	-	chr1.2:70881064-70881083	MS.gene60695:CDS	25.0%
	CAGAAACTTATCCTTATGTA+TGG	-	chr1.2:70881109-70881128	MS.gene60695:CDS	30.0%
	GAAACTTATCCTTATGTATG+GGG	-	chr1.2:70881111-70881130	MS.gene60695:CDS	30.0%
	GGTTCCTATATTCTTGTATA+TGG	-	chr1.2:70881132-70881151	MS.gene60695:CDS	30.0%
	TCCTATATTCTTGTATATGG+CGG	-	chr1.2:70881135-70881154	MS.gene60695:CDS	30.0%
!!!	GCTTGTTGCTTTTCATTATT+TGG	+	chr1.2:70880973-70880992	None:intergenic	30.0%
	ATATAGGAACCCCATACATA+AGG	+	chr1.2:70881123-70881142	None:intergenic	35.0%
	TCCGCCATATACAAGAATAT+AGG	+	chr1.2:70881139-70881158	None:intergenic	35.0%
	TTCTAGTAATTCTGCCAAAG+TGG	-	chr1.2:70881278-70881297	MS.gene60695:CDS	35.0%
!!	ATTCTTGTATATGGCGGATT+TGG	-	chr1.2:70881141-70881160	MS.gene60695:CDS	35.0%
!!!	GCTTGCTGCTTTTCATTATT+TGG	+	chr1.2:70880802-70880821	None:intergenic	35.0%
	ACTACGTGAGATGGTTGAAA+AGG	-	chr1.2:70881233-70881252	MS.gene60695:CDS	40.0%
	TAGAAAGCTTCTTAGGACAG+AGG	-	chr1.2:70881185-70881204	MS.gene60695:CDS	40.0%
	TCTGCCAAAGTGGTTGAAAA+GGG	-	chr1.2:70881288-70881307	MS.gene60695:CDS	40.0%
	TGGATAAGTGCTTCAGAACT+TGG	+	chr1.2:70880826-70880845	None:intergenic	40.0%
	TTCTGCCAAAGTGGTTGAAA+AGG	-	chr1.2:70881287-70881306	MS.gene60695:CDS	40.0%
!	GAAGCACTTATCCAGAAAAC+TGG	-	chr1.2:70880832-70880851	MS.gene60695:CDS	40.0%
!	TGCAGGCTTTATGGATTCAT+TGG	-	chr1.2:70881056-70881075	MS.gene60695:CDS	40.0%
!!	TATATGGCGGATTTGGTCTT+TGG	-	chr1.2:70881148-70881167	MS.gene60695:CDS	40.0%
	ACTTATCCAGAAAACTGGCG+AGG	-	chr1.2:70880837-70880856	MS.gene60695:CDS	45.0%
	GGAAGGAATCTCAGAAGATG+TGG	+	chr1.2:70881316-70881335	None:intergenic	45.0%
	TCAAGCAAGACTACGTGAGA+TGG	-	chr1.2:70881224-70881243	MS.gene60695:CDS	45.0%
!	GGAACCCTTTTCAACCACTT+TGG	+	chr1.2:70881295-70881314	None:intergenic	45.0%
!!	TGGTTGATTTGCCATGTCCT+TGG	+	chr1.2:70880953-70880972	None:intergenic	45.0%
	AAGTTCTGCAGCACCTATCC+TGG	-	chr1.2:70880996-70881015	MS.gene60695:CDS	50.0%
	ACCTATCCTGGAAACTGGTG+AGG	-	chr1.2:70881008-70881027	MS.gene60695:CDS	50.0%
	ACTGGCGAGGATCAAGTAGT+CGG	-	chr1.2:70880850-70880869	MS.gene60695:CDS	50.0%
	ACTGGTGAGGACCAAGTAGT+CGG	-	chr1.2:70881021-70881040	MS.gene60695:CDS	50.0%
	AGCTTCTTAGGACAGAGGAC+AGG	-	chr1.2:70881190-70881209	MS.gene60695:CDS	50.0%
	CTCTTCTGCTACGGGCTTTA+TGG	-	chr1.2:70880876-70880895	MS.gene60695:CDS	50.0%
	GAATCCATAAAGCCTGCAGC+AGG	+	chr1.2:70881054-70881073	None:intergenic	50.0%
	GCTTCTTAGGACAGAGGACA+GGG	-	chr1.2:70881191-70881210	MS.gene60695:CDS	50.0%
	TCCTCACCAGTTTCCAGGAT+AGG	+	chr1.2:70881012-70881031	None:intergenic	50.0%
	TTAGGACAGAGGACAGGGTA+CGG	-	chr1.2:70881196-70881215	MS.gene60695:CDS	50.0%
!	AGGATAGGTGCTGCAGAACT+TGG	+	chr1.2:70880997-70881016	None:intergenic	50.0%
!	CTTGATCCTCGCCAGTTTTC+TGG	+	chr1.2:70880846-70880865	None:intergenic	50.0%
!	TGATTTGCCATGTCCTTGGC+TGG	+	chr1.2:70880949-70880968	None:intergenic	50.0%
	AGTCGGTGGCTCTTCTGCTA+CGG	-	chr1.2:70880867-70880886	MS.gene60695:CDS	55.0%
	CATAAAGCCTGCAGCAGGAG+GGG	+	chr1.2:70881049-70881068	None:intergenic	55.0%
	CCATAAAGCCTGCAGCAGGA+GGG	+	chr1.2:70881050-70881069	None:intergenic	55.0%
	CTTGGTCCTCACCAGTTTCC+AGG	+	chr1.2:70881017-70881036	None:intergenic	55.0%
	GAACACTCCAGCCAAGGACA+TGG	-	chr1.2:70880939-70880958	MS.gene60695:CDS	55.0%
	GCAGCACCTATCCTGGAAAC+TGG	-	chr1.2:70881003-70881022	MS.gene60695:CDS	55.0%
	TAGCTGGAACACTCCAGCCA+AGG	-	chr1.2:70880933-70880952	MS.gene60695:CDS	55.0%
	TAGGACAGAGGACAGGGTAC+GGG	-	chr1.2:70881197-70881216	MS.gene60695:CDS	55.0%
	TCCATAAAGCCTGCAGCAGG+AGG	+	chr1.2:70881051-70881070	None:intergenic	55.0%
	CCCTCCTGCTGCAGGCTTTA+TGG	-	chr1.2:70881047-70881066	MS.gene60695:CDS	60.0%
	GGCGAGGATCAAGTAGTCGG+TGG	-	chr1.2:70880853-70880872	MS.gene60695:CDS	60.0%
	GTCGGTGGCTCTTCTGCTAC+GGG	-	chr1.2:70880868-70880887	MS.gene60695:CDS	60.0%
!!	CAGGAGGGGCACCGACTACT+TGG	+	chr1.2:70881035-70881054	None:intergenic	65.0%
!	GTCGGTGCCCCTCCTGCTGC+AGG	-	chr1.2:70881039-70881058	MS.gene60695:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	70880787	70881341	70880787	ID=MS.gene60695
chr1.2	mRNA	70880787	70881341	70880787	ID=MS.gene60695.t1;Parent=MS.gene60695
chr1.2	exon	70880787	70881341	70880787	ID=MS.gene60695.t1.exon1;Parent=MS.gene60695.t1
chr1.2	CDS	70880787	70881341	70880787	ID=cds.MS.gene60695.t1;Parent=MS.gene60695.t1

Gene Sequence

>MS.gene60695

ATGGCAAATCAACCAAATAATGAAAAGCAGCAAGCACCAAGTTCTGAAGCACTTATCCAGAAAACTGGCGAGGATCAAGTAGTCGGTGGCTCTTCTGCTACGGGCTTTATGGATTCATTGAAAAATCGGTATCAAAAAATCATAGATAGCTGGAACACTCCAGCCAAGGACATGGCAAATCAACCAAATAATGAAAAGCAACAAGCACCAAGTTCTGCAGCACCTATCCTGGAAACTGGTGAGGACCAAGTAGTCGGTGCCCCTCCTGCTGCAGGCTTTATGGATTCATTGGAAAATCGGTATAAAAAAATCAAAGAGCATGCAGAAACTTATCCTTATGTATGGGGTTCCTATATTCTTGTATATGGCGGATTTGGTCTTTGGTTTGCCTATAGATATAGAAAGCTTCTTAGGACAGAGGACAGGGTACGGGCACTTCAAGCAAGACTACGTGAGATGGTTGAAAAGGATAAGATTGTTGAAGCTGAAAATTCTAGTAATTCTGCCAAAGTGGTTGAAAAGGGTTCCACATCTTCTGAGATTCCTTCCAAGTAG

Protein sequence

>MS.gene60695.t1

MANQPNNEKQQAPSSEALIQKTGEDQVVGGSSATGFMDSLKNRYQKIIDSWNTPAKDMANQPNNEKQQAPSSAAPILETGEDQVVGAPPAAGFMDSLENRYKKIKEHAETYPYVWGSYILVYGGFGLWFAYRYRKLLRTEDRVRALQARLREMVEKDKIVEAENSSNSAKVVEKGSTSSEIPSK