AlfalfaGEDB | MS.gene61161

M. sativa cultivar XinJiangDaYe / MS.gene61161

KEGG: -
GO: -
PFAM: PF05678.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61161.t1	XP_003604356.1	87.7	195	7	3	1	193	1	180	1.60E-51	212.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61161.t1	Q9M8L3	35.7	185	87	5	12	192	11	167	1.8e-14	80.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61161.t1	G7JGW0	87.7	195	7	3	1	193	1	180	1.2e-51	212.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050165	MS.gene61161	-0.816864	4.44E-52	-1.69E-46
MS.gene050333	MS.gene61161	-0.815714	8.03E-52	-1.69E-46
MS.gene051949	MS.gene61161	-0.803491	3.40E-49	-1.69E-46
MS.gene059079	MS.gene61161	-0.811333	7.38E-51	-1.69E-46
MS.gene060762	MS.gene61161	-0.809582	1.76E-50	-1.69E-46
MS.gene060838	MS.gene61161	0.850086	2.13E-60	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61161.t1	MTR_4g009950	89.231	195	4	3	1	193	1	180	7.55e-118	332
MS.gene61161.t1	MTR_2g079120	36.598	194	95	8	16	193	65	246	1.66e-13	67.0
MS.gene61161.t1	MTR_2g013950	32.653	196	80	7	16	187	83	250	3.16e-13	66.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61161.t1	AT1G80450	40.385	208	58	7	4	192	7	167	2.03e-31	112
MS.gene61161.t1	AT1G28280	34.694	196	88	9	16	187	57	236	1.20e-14	70.5
MS.gene61161.t1	AT1G28280	34.694	196	88	9	16	187	57	236	1.24e-14	70.1
MS.gene61161.t1	AT5G08480	34.043	188	85	6	11	189	12	169	5.95e-13	64.3
MS.gene61161.t1	AT5G08480	34.043	188	85	6	11	189	12	169	5.95e-13	64.3
MS.gene61161.t1	AT3G15300	33.668	199	83	9	16	187	37	213	2.65e-12	63.5
MS.gene61161.t1	AT2G33780	31.551	187	98	8	16	192	36	202	1.88e-11	60.8
MS.gene61161.t1	AT5G53830	34.634	205	95	11	16	191	49	243	4.57e-11	60.5

Find 44 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGTGGAGGACGGTGGGCT+TGG	0.256659	4.2:+90206976	None:intergenic
GAGGACGGTGGGCTTGGGCT+TGG	0.257828	4.2:+90206982	None:intergenic
CAACATGCGAAGTGTTATTA+TGG	0.268568	4.2:-90206781	MS.gene61161:CDS
TCATGTTATTGTTGTTGTTT+TGG	0.291134	4.2:+90206844	None:intergenic
AGGACGGTGGGCTTGGGCTT+GGG	0.306796	4.2:+90206983	None:intergenic
TAATATTCCAATGGTGAAAC+TGG	0.334222	4.2:+90206753	None:intergenic
TGGTGGAGGACGGTGGGCTT+GGG	0.338898	4.2:+90206977	None:intergenic
TTGTGTTTGGTTGTGTTGTT+AGG	0.343814	4.2:+90207120	None:intergenic
GGTTCCATGGTGGTTGTTGT+TGG	0.392587	4.2:+90206957	None:intergenic
TCCATGGTGGTTGTTGTTGG+TGG	0.405900	4.2:+90206960	None:intergenic
GCTTGTACAAAGGTTGTGTT+TGG	0.409951	4.2:+90207107	None:intergenic
ACAACTTGTCGGAAATTTGA+TGG	0.417823	4.2:+90207080	None:intergenic
GCGTTCGTGGAGTTTGAAGT+TGG	0.435488	4.2:+90206914	None:intergenic
TAAGAATGAGATTGAGAGGA+AGG	0.443832	4.2:+90206588	None:intergenic
CGTTCGTGGAGTTTGAAGTT+GGG	0.446220	4.2:+90206915	None:intergenic
TGGTTGTTGTTGGTGGAGGA+CGG	0.455302	4.2:+90206967	None:intergenic
TAAGCCCATTGGTGGTTCCA+TGG	0.464436	4.2:+90206944	None:intergenic
ACCATGCATTCACCAACATA+TGG	0.465787	4.2:-90207149	MS.gene61161:CDS
GGCTTCACCAGTTTCACCAT+TGG	0.477870	4.2:-90206760	MS.gene61161:CDS
TGTTGTTGGTGGAGGACGGT+GGG	0.481918	4.2:+90206971	None:intergenic
AAAACTCCAACTCAACGTTT+CGG	0.499017	4.2:-90206883	MS.gene61161:CDS
AGACGAGAAGGGAGTGTGAG+TGG	0.503781	4.2:+90207011	None:intergenic
TGATGGGTCTTGTGATGCTC+CGG	0.504118	4.2:+90207046	None:intergenic
ATGGTGGTTGTTGTTGGTGG+AGG	0.515633	4.2:+90206963	None:intergenic
AACCACCATGGAACCACCAA+TGG	0.516253	4.2:-90206949	MS.gene61161:CDS
ACCACCATGGAACCACCAAT+GGG	0.535319	4.2:-90206948	MS.gene61161:CDS
GTTTGGTTGTGTTGTTAGGT+CGG	0.535973	4.2:+90207124	None:intergenic
GCTTGGGCTGTTAGACGAGA+AGG	0.539771	4.2:+90206999	None:intergenic
TTGTTGTTGGTGGAGGACGG+TGG	0.540140	4.2:+90206970	None:intergenic
TAATTAAGAATGAGATTGAG+AGG	0.547532	4.2:+90206584	None:intergenic
TGATGGGTCTGCTTGTACAA+AGG	0.550070	4.2:+90207097	None:intergenic
GGACTATAGTAATATTCCAA+TGG	0.550459	4.2:+90206744	None:intergenic
CAACTTGTCGGAAATTTGAT+GGG	0.562610	4.2:+90207081	None:intergenic
TGTTGAGAAGCTCTAGGAGA+GGG	0.563092	4.2:+90206651	None:intergenic
TGAGAGGAAGGAGAATGCAA+AGG	0.574115	4.2:+90206600	None:intergenic
GGTTGTTGTTGAGAAGCTCT+AGG	0.576546	4.2:+90206645	None:intergenic
GTTAGGTCGGATCCATATGT+TGG	0.606638	4.2:+90207137	None:intergenic
AAAAGAGGTAAAAGTTCAGG+TGG	0.608567	4.2:+90206624	None:intergenic
TCCATATGTTGGTGAATGCA+TGG	0.614670	4.2:+90207148	None:intergenic
CTTGGGCTGTTAGACGAGAA+GGG	0.624849	4.2:+90207000	None:intergenic
TTGTTGAGAAGCTCTAGGAG+AGG	0.644100	4.2:+90206650	None:intergenic
TCCACCAACAACAACCACCA+TGG	0.648779	4.2:-90206961	MS.gene61161:CDS
GCCCATTGGTGGTTCCATGG+TGG	0.657294	4.2:+90206947	None:intergenic
GTTGAGAAGCTCTAGGAGAG+GGG	0.657383	4.2:+90206652	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCTTTTTCTTCATTTTCATT+TGG	+	chr4.2:90207022-90207041	None:intergenic	20.0%
!!!	TCATGTTATTGTTGTTGTTT+TGG	+	chr4.2:90206892-90206911	None:intergenic	25.0%
!!!	TCATTTTCATTTGGTGATTT+TGG	+	chr4.2:90207013-90207032	None:intergenic	25.0%
	GAAAAAGTGATAGCTGAAAA+AGG	-	chr4.2:90207046-90207065	MS.gene61161:CDS	30.0%
	GGACTATAGTAATATTCCAA+TGG	+	chr4.2:90206992-90207011	None:intergenic	30.0%
	TAATATTCCAATGGTGAAAC+TGG	+	chr4.2:90206983-90207002	None:intergenic	30.0%
!!	TTAGTTTTTGGACAACTTGT+CGG	+	chr4.2:90206667-90206686	None:intergenic	30.0%
!!!	TTGTTGTTTTGGAACATCAT+GGG	+	chr4.2:90206881-90206900	None:intergenic	30.0%
	AAAACTCCAACTCAACGTTT+CGG	-	chr4.2:90206850-90206869	MS.gene61161:CDS	35.0%
	AAAAGAGGTAAAAGTTCAGG+TGG	+	chr4.2:90207112-90207131	None:intergenic	35.0%
	ACAACTTGTCGGAAATTTGA+TGG	+	chr4.2:90206656-90206675	None:intergenic	35.0%
	CAACTTGTCGGAAATTTGAT+GGG	+	chr4.2:90206655-90206674	None:intergenic	35.0%
	CAAGTTGTCCAAAAACTAAC+CGG	-	chr4.2:90206668-90206687	MS.gene61161:CDS	35.0%
	GGAAAAAGAGGTAAAAGTTC+AGG	+	chr4.2:90207115-90207134	None:intergenic	35.0%
	TAAGAATGAGATTGAGAGGA+AGG	+	chr4.2:90207148-90207167	None:intergenic	35.0%
!	CAACATGCGAAGTGTTATTA+TGG	-	chr4.2:90206952-90206971	MS.gene61161:CDS	35.0%
!	TTGTGTTTGGTTGTGTTGTT+AGG	+	chr4.2:90206616-90206635	None:intergenic	35.0%
!!!	GTTGTTGTTTTGGAACATCA+TGG	+	chr4.2:90206882-90206901	None:intergenic	35.0%
!!!	TGAGTTGGAGTTTTTTAGAG+CGG	+	chr4.2:90206844-90206863	None:intergenic	35.0%
!!!	TTGGGTTTTTTTAAGCCCAT+TGG	+	chr4.2:90206803-90206822	None:intergenic	35.0%
	GCTTGTACAAAGGTTGTGTT+TGG	+	chr4.2:90206629-90206648	None:intergenic	40.0%
	GGAGAATGCAAAGGAAAAAG+AGG	+	chr4.2:90207127-90207146	None:intergenic	40.0%
!	GAGTGTGAGTGGAAGTTTTT+GGG	+	chr4.2:90206714-90206733	None:intergenic	40.0%
!	GATTTTCCGAAACGTTGAGT+TGG	+	chr4.2:90206859-90206878	None:intergenic	40.0%
!	GTTTGGTTGTGTTGTTAGGT+CGG	+	chr4.2:90206612-90206631	None:intergenic	40.0%
!!!	GGTTTTTTTAAGCCCATTGG+TGG	+	chr4.2:90206800-90206819	None:intergenic	40.0%
	GTTAGGTCGGATCCATATGT+TGG	+	chr4.2:90206599-90206618	None:intergenic	45.0%
	TGAGAGGAAGGAGAATGCAA+AGG	+	chr4.2:90207136-90207155	None:intergenic	45.0%
!	CGTTCGTGGAGTTTGAAGTT+GGG	+	chr4.2:90206821-90206840	None:intergenic	45.0%
!	GGAGTGTGAGTGGAAGTTTT+TGG	+	chr4.2:90206715-90206734	None:intergenic	45.0%
!	GGTTGTTGTTGAGAAGCTCT+AGG	+	chr4.2:90207091-90207110	None:intergenic	45.0%
!	TGATGGGTCTGCTTGTACAA+AGG	+	chr4.2:90206639-90206658	None:intergenic	45.0%
!!	AGTGGAAGTTTTTGGGCTGA+TGG	+	chr4.2:90206707-90206726	None:intergenic	45.0%
!!	GTGGAAGTTTTTGGGCTGAT+GGG	+	chr4.2:90206706-90206725	None:intergenic	45.0%
!!	TGTTGAGAAGCTCTAGGAGA+GGG	+	chr4.2:90207085-90207104	None:intergenic	45.0%
!!	TTGTTGAGAAGCTCTAGGAG+AGG	+	chr4.2:90207086-90207105	None:intergenic	45.0%
!!!	GTGATGCTCCGGTTAGTTTT+TGG	+	chr4.2:90206679-90206698	None:intergenic	45.0%
	AACCACCATGGAACCACCAA+TGG	-	chr4.2:90206784-90206803	MS.gene61161:CDS	50.0%
	ACCACCATGGAACCACCAAT+GGG	-	chr4.2:90206785-90206804	MS.gene61161:CDS	50.0%
	CTTGGGCTGTTAGACGAGAA+GGG	+	chr4.2:90206736-90206755	None:intergenic	50.0%
	GGCTTCACCAGTTTCACCAT+TGG	-	chr4.2:90206973-90206992	MS.gene61161:CDS	50.0%
	GGTTCCATGGTGGTTGTTGT+TGG	+	chr4.2:90206779-90206798	None:intergenic	50.0%
	TCCACCAACAACAACCACCA+TGG	-	chr4.2:90206772-90206791	MS.gene61161:CDS	50.0%
!	ATGGTGGTTGTTGTTGGTGG+AGG	+	chr4.2:90206773-90206792	None:intergenic	50.0%
!	GCGTTCGTGGAGTTTGAAGT+TGG	+	chr4.2:90206822-90206841	None:intergenic	50.0%
!	TAAGCCCATTGGTGGTTCCA+TGG	+	chr4.2:90206792-90206811	None:intergenic	50.0%
!	TCCATGGTGGTTGTTGTTGG+TGG	+	chr4.2:90206776-90206795	None:intergenic	50.0%
!	TGATGGGTCTTGTGATGCTC+CGG	+	chr4.2:90206690-90206709	None:intergenic	50.0%
!	TGGTTGTTGTTGGTGGAGGA+CGG	+	chr4.2:90206769-90206788	None:intergenic	50.0%
!!	GTTGAGAAGCTCTAGGAGAG+GGG	+	chr4.2:90207084-90207103	None:intergenic	50.0%
!!!	GTTTTTTAGAGCGGCGTTCG+TGG	+	chr4.2:90206835-90206854	None:intergenic	50.0%
	GCTTGGGCTGTTAGACGAGA+AGG	+	chr4.2:90206737-90206756	None:intergenic	55.0%
!	AGACGAGAAGGGAGTGTGAG+TGG	+	chr4.2:90206725-90206744	None:intergenic	55.0%
!	TGTTGTTGGTGGAGGACGGT+GGG	+	chr4.2:90206765-90206784	None:intergenic	55.0%
!	TTGTTGTTGGTGGAGGACGG+TGG	+	chr4.2:90206766-90206785	None:intergenic	55.0%
!	GCCCATTGGTGGTTCCATGG+TGG	+	chr4.2:90206789-90206808	None:intergenic	60.0%
!	AGGACGGTGGGCTTGGGCTT+GGG	+	chr4.2:90206753-90206772	None:intergenic	65.0%
!	TGGTGGAGGACGGTGGGCTT+GGG	+	chr4.2:90206759-90206778	None:intergenic	65.0%
!	TTGGTGGAGGACGGTGGGCT+TGG	+	chr4.2:90206760-90206779	None:intergenic	65.0%
!	GAGGACGGTGGGCTTGGGCT+TGG	+	chr4.2:90206754-90206773	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	90206587	90207168	90206587	ID=MS.gene61161
chr4.2	mRNA	90206587	90207168	90206587	ID=MS.gene61161.t1;Parent=MS.gene61161
chr4.2	exon	90206587	90207168	90206587	ID=MS.gene61161.t1.exon1;Parent=MS.gene61161.t1
chr4.2	CDS	90206587	90207168	90206587	ID=cds.MS.gene61161.t1;Parent=MS.gene61161.t1

Gene Sequence

>MS.gene61161

ATGCATTCACCAACATATGGATCCGACCTAACAACACAACCAAACACAACCTTTGTACAAGCAGACCCATCAAATTTCCGACAAGTTGTCCAAAAACTAACCGGAGCATCACAAGACCCATCAGCCCAAAAACTTCCACTCACACTCCCTTCTCGTCTAACAGCCCAAGCCCAAGCCCACCGTCCTCCACCAACAACAACCACCATGGAACCACCAATGGGCTTAAAAAAACCCAACTTCAAACTCCACGAACGCCGCTCTAAAAAACTCCAACTCAACGTTTCGGAAAATCCCATGATGTTCCAAAACAACAACAATAACATGATGATGATGTTAATGAATAGTAACAACAACAACAACAACAACAACATGCGAAGTGTTATTATGGCTTCACCAGTTTCACCATTGGAATATTACTATAGTCCAAAATCACCAAATGAAAATGAAGAAAAAGAAGAAGAAAAAGTGATAGCTGAAAAAGGTTTCTATTTGCACCCCTCTCCTAGAGCTTCTCAACAACAACCACCTGAACTTTTACCTCTTTTTCCTTTGCATTCTCCTTCCTCTCAATCTCATTCTTAA

Protein sequence

>MS.gene61161.t1

MHSPTYGSDLTTQPNTTFVQADPSNFRQVVQKLTGASQDPSAQKLPLTLPSRLTAQAQAHRPPPTTTTMEPPMGLKKPNFKLHERRSKKLQLNVSENPMMFQNNNNNMMMMLMNSNNNNNNNNMRSVIMASPVSPLEYYYSPKSPNENEEKEEEKVIAEKGFYLHPSPRASQQQPPELLPLFPLHSPSSQSHS