AlfalfaGEDB | MS.gene060838

M. sativa cultivar XinJiangDaYe / MS.gene060838

KEGG: -
GO: -
PFAM: PF05678.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060838.t1	XP_003604356.1	89.1	192	7	3	1	190	1	180	7.10E-52	213.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060838.t1	Q9M8L3	36.3	182	87	5	12	189	11	167	7.7e-15	82.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060838.t1	G7JGW0	89.1	192	7	3	1	190	1	180	5.1e-52	213.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055751	MS.gene060838	-0.803453	3.46E-49	-1.69E-46
MS.gene059079	MS.gene060838	-0.80387	2.84E-49	-1.69E-46
MS.gene060762	MS.gene060838	-0.81395	1.97E-51	-1.69E-46
MS.gene060838	MS.gene062903	-0.811859	5.67E-51	-1.69E-46
MS.gene060838	MS.gene063080	-0.823707	1.20E-53	-1.69E-46
MS.gene060838	MS.gene063965	-0.803558	3.29E-49	-1.69E-46
MS.gene060838	MS.gene23334	-0.812695	3.72E-51	-1.69E-46
MS.gene060838	MS.gene54782	-0.805245	1.46E-49	-1.69E-46
MS.gene060838	MS.gene58012	-0.801152	1.03E-48	-1.69E-46
MS.gene060838	MS.gene58060	-0.805481	1.31E-49	-1.69E-46
MS.gene060838	MS.gene61161	0.850086	2.13E-60	-1.69E-46
MS.gene060838	MS.gene67656	-0.802371	5.79E-49	-1.69E-46
MS.gene060838	MS.gene90576	-0.800272	1.56E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060838.t1	MTR_4g009950	91.146	192	3	3	1	190	1	180	3.31e-119	335
MS.gene060838.t1	MTR_2g013950	35.450	189	81	10	16	184	83	250	1.12e-13	67.8
MS.gene060838.t1	MTR_2g079120	36.598	194	92	8	16	190	65	246	3.66e-13	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060838.t1	AT1G80450	41.709	199	55	7	11	189	10	167	3.81e-31	112
MS.gene060838.t1	AT1G28280	35.938	192	88	10	16	184	57	236	6.69e-14	68.2
MS.gene060838.t1	AT1G28280	35.938	192	88	10	16	184	57	236	7.58e-14	68.2
MS.gene060838.t1	AT5G08480	34.054	185	86	5	11	186	12	169	2.65e-13	65.1
MS.gene060838.t1	AT5G08480	34.054	185	86	5	11	186	12	169	2.65e-13	65.1
MS.gene060838.t1	AT3G15300	33.161	193	89	8	16	184	37	213	1.01e-11	62.0
MS.gene060838.t1	AT2G33780	32.065	184	98	8	16	189	36	202	1.05e-11	61.6
MS.gene060838.t1	AT5G53830	34.634	205	92	11	16	188	49	243	4.70e-11	60.5

Find 44 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGTGGAGGACGGTGGGCT+TGG	0.256659	4.3:+83514439	None:intergenic
GAGGACGGTGGGCTTGGGCT+TGG	0.257828	4.3:+83514445	None:intergenic
CAACATGCGAAGTGTTATTA+TGG	0.268568	4.3:-83514253	MS.gene060838:CDS
TCATGTTATTGTTGTTGTTT+TGG	0.291134	4.3:+83514307	None:intergenic
AGGACGGTGGGCTTGGGCTT+GGG	0.306796	4.3:+83514446	None:intergenic
TAATATTCCAATGGTGAAAC+TGG	0.334222	4.3:+83514225	None:intergenic
TGGTGGAGGACGGTGGGCTT+GGG	0.338898	4.3:+83514440	None:intergenic
TTGTGTTTGGTTGTGTTGTT+AGG	0.343814	4.3:+83514583	None:intergenic
GGTTCCATGGTGGTTGTTGT+TGG	0.392587	4.3:+83514420	None:intergenic
TCCATGGTGGTTGTTGTTGG+TGG	0.405900	4.3:+83514423	None:intergenic
GCTTGTACAAAGGTTGTGTT+TGG	0.409951	4.3:+83514570	None:intergenic
ACAACTTGTCGGAAATTTGA+TGG	0.417823	4.3:+83514543	None:intergenic
GCGTTCGTGGAGTTTGAAGT+TGG	0.435488	4.3:+83514377	None:intergenic
TAAGAATGAGATTGAGAGGA+AGG	0.443832	4.3:+83514060	None:intergenic
CGTTCGTGGAGTTTGAAGTT+GGG	0.446220	4.3:+83514378	None:intergenic
TGGTTGTTGTTGGTGGAGGA+CGG	0.455302	4.3:+83514430	None:intergenic
TAAGCCCATTGGTGGTTCCA+TGG	0.464436	4.3:+83514407	None:intergenic
ACCATGCATTCACCAACATA+TGG	0.465787	4.3:-83514612	MS.gene060838:CDS
GGCTTCACCAGTTTCACCAT+TGG	0.477870	4.3:-83514232	MS.gene060838:CDS
TGTTGTTGGTGGAGGACGGT+GGG	0.481918	4.3:+83514434	None:intergenic
AAAACTCCAACTCAACGTTT+CGG	0.499017	4.3:-83514346	MS.gene060838:CDS
AGACGAGAAGGGAGTGTGAG+TGG	0.503781	4.3:+83514474	None:intergenic
TGATGGGTCTTGTGATGCTC+CGG	0.504118	4.3:+83514509	None:intergenic
ATGGTGGTTGTTGTTGGTGG+AGG	0.515633	4.3:+83514426	None:intergenic
AACCACCATGGAACCACCAA+TGG	0.516253	4.3:-83514412	MS.gene060838:CDS
ACCACCATGGAACCACCAAT+GGG	0.535319	4.3:-83514411	MS.gene060838:CDS
GTTTGGTTGTGTTGTTAGGT+CGG	0.535973	4.3:+83514587	None:intergenic
GCTTGGGCTGTTAGACGAGA+AGG	0.539771	4.3:+83514462	None:intergenic
TTGTTGTTGGTGGAGGACGG+TGG	0.540140	4.3:+83514433	None:intergenic
TAATTAAGAATGAGATTGAG+AGG	0.547532	4.3:+83514056	None:intergenic
TGATGGGTCTGCTTGTACAA+AGG	0.550070	4.3:+83514560	None:intergenic
GGACTATAGTAATATTCCAA+TGG	0.550459	4.3:+83514216	None:intergenic
CAACTTGTCGGAAATTTGAT+GGG	0.562610	4.3:+83514544	None:intergenic
TGTTGAGAAGCTCTAGGAGA+GGG	0.563092	4.3:+83514123	None:intergenic
TGAGAGGAAGGAGAATGCAA+AGG	0.574115	4.3:+83514072	None:intergenic
GGTTGTTGTTGAGAAGCTCT+AGG	0.576546	4.3:+83514117	None:intergenic
GTTAGGTCGGATCCATATGT+TGG	0.606638	4.3:+83514600	None:intergenic
AAAAGAGGTAAAAGTTCAGG+TGG	0.608567	4.3:+83514096	None:intergenic
TCCATATGTTGGTGAATGCA+TGG	0.614670	4.3:+83514611	None:intergenic
CTTGGGCTGTTAGACGAGAA+GGG	0.624849	4.3:+83514463	None:intergenic
TTGTTGAGAAGCTCTAGGAG+AGG	0.644100	4.3:+83514122	None:intergenic
TCCACCAACAACAACCACCA+TGG	0.648779	4.3:-83514424	MS.gene060838:CDS
GCCCATTGGTGGTTCCATGG+TGG	0.657294	4.3:+83514410	None:intergenic
GTTGAGAAGCTCTAGGAGAG+GGG	0.657383	4.3:+83514124	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCTTTTTCTTCATTTTCATG+TGG	+	chr4.3:83514485-83514504	None:intergenic	25.0%
!!!	TCATGTTATTGTTGTTGTTT+TGG	+	chr4.3:83514364-83514383	None:intergenic	25.0%
	GAAAAAGTGATAGCTGAAAA+AGG	-	chr4.3:83514509-83514528	MS.gene060838:CDS	30.0%
	GGACTATAGTAATATTCCAA+TGG	+	chr4.3:83514455-83514474	None:intergenic	30.0%
	TAATATTCCAATGGTGAAAC+TGG	+	chr4.3:83514446-83514465	None:intergenic	30.0%
!	TCATTTTCATGTGGTGATTT+TGG	+	chr4.3:83514476-83514495	None:intergenic	30.0%
!!	TTAGTTTTTGGACAACTTGT+CGG	+	chr4.3:83514139-83514158	None:intergenic	30.0%
!!!	TTGTTGTTTTGGAACATCAT+GGG	+	chr4.3:83514353-83514372	None:intergenic	30.0%
	AAAACTCCAACTCAACGTTT+CGG	-	chr4.3:83514322-83514341	MS.gene060838:CDS	35.0%
	AAAAGAGGTAAAAGTTCAGG+TGG	+	chr4.3:83514575-83514594	None:intergenic	35.0%
	ACAACTTGTCGGAAATTTGA+TGG	+	chr4.3:83514128-83514147	None:intergenic	35.0%
	CAACTTGTCGGAAATTTGAT+GGG	+	chr4.3:83514127-83514146	None:intergenic	35.0%
	CAAGTTGTCCAAAAACTAAC+CGG	-	chr4.3:83514140-83514159	MS.gene060838:CDS	35.0%
	GGAAAAAGAGGTAAAAGTTC+AGG	+	chr4.3:83514578-83514597	None:intergenic	35.0%
	TAAGAATGAGATTGAGAGGA+AGG	+	chr4.3:83514611-83514630	None:intergenic	35.0%
!	CAACATGCGAAGTGTTATTA+TGG	-	chr4.3:83514415-83514434	MS.gene060838:CDS	35.0%
!	TTGTGTTTGGTTGTGTTGTT+AGG	+	chr4.3:83514088-83514107	None:intergenic	35.0%
!!!	GTTGTTGTTTTGGAACATCA+TGG	+	chr4.3:83514354-83514373	None:intergenic	35.0%
!!!	TGAGTTGGAGTTTTTTAGAG+CGG	+	chr4.3:83514316-83514335	None:intergenic	35.0%
!!!	TTGGGTTTTTTTAAGCCCAT+TGG	+	chr4.3:83514275-83514294	None:intergenic	35.0%
	GCTTGTACAAAGGTTGTGTT+TGG	+	chr4.3:83514101-83514120	None:intergenic	40.0%
	GGAGAATGCAAAGGAAAAAG+AGG	+	chr4.3:83514590-83514609	None:intergenic	40.0%
!	GAGTGTGAGTGGAAGTTTTT+GGG	+	chr4.3:83514186-83514205	None:intergenic	40.0%
!	GATTTTCCGAAACGTTGAGT+TGG	+	chr4.3:83514331-83514350	None:intergenic	40.0%
!	GTTTGGTTGTGTTGTTAGGT+CGG	+	chr4.3:83514084-83514103	None:intergenic	40.0%
!!!	GGTTTTTTTAAGCCCATTGG+TGG	+	chr4.3:83514272-83514291	None:intergenic	40.0%
	GTTAGGTCGGATCCATATGT+TGG	+	chr4.3:83514071-83514090	None:intergenic	45.0%
	TGAGAGGAAGGAGAATGCAA+AGG	+	chr4.3:83514599-83514618	None:intergenic	45.0%
!	CGTTCGTGGAGTTTGAAGTT+GGG	+	chr4.3:83514293-83514312	None:intergenic	45.0%
!	GGAGTGTGAGTGGAAGTTTT+TGG	+	chr4.3:83514187-83514206	None:intergenic	45.0%
!	GGTTGTTGTTGAGAAGCTCT+AGG	+	chr4.3:83514554-83514573	None:intergenic	45.0%
!	TGATGGGTCTGCTTGTACAA+AGG	+	chr4.3:83514111-83514130	None:intergenic	45.0%
!!	AGTGGAAGTTTTTGGGCTGA+TGG	+	chr4.3:83514179-83514198	None:intergenic	45.0%
!!	GTGGAAGTTTTTGGGCTGAT+GGG	+	chr4.3:83514178-83514197	None:intergenic	45.0%
!!	TGTTGAGAAGCTCTAGGAGA+GGG	+	chr4.3:83514548-83514567	None:intergenic	45.0%
!!	TTGTTGAGAAGCTCTAGGAG+AGG	+	chr4.3:83514549-83514568	None:intergenic	45.0%
!!!	GTGATGCTCCGGTTAGTTTT+TGG	+	chr4.3:83514151-83514170	None:intergenic	45.0%
	AACCACCATGGAACCACCAA+TGG	-	chr4.3:83514256-83514275	MS.gene060838:CDS	50.0%
	ACCACCATGGAACCACCAAT+GGG	-	chr4.3:83514257-83514276	MS.gene060838:CDS	50.0%
	CTTGGGCTGTTAGACGAGAA+GGG	+	chr4.3:83514208-83514227	None:intergenic	50.0%
	GGCTTCACCAGTTTCACCAT+TGG	-	chr4.3:83514436-83514455	MS.gene060838:CDS	50.0%
	GGTTCCATGGTGGTTGTTGT+TGG	+	chr4.3:83514251-83514270	None:intergenic	50.0%
	TCCACCAACAACAACCACCA+TGG	-	chr4.3:83514244-83514263	MS.gene060838:CDS	50.0%
!	ATGGTGGTTGTTGTTGGTGG+AGG	+	chr4.3:83514245-83514264	None:intergenic	50.0%
!	GCGTTCGTGGAGTTTGAAGT+TGG	+	chr4.3:83514294-83514313	None:intergenic	50.0%
!	TAAGCCCATTGGTGGTTCCA+TGG	+	chr4.3:83514264-83514283	None:intergenic	50.0%
!	TCCATGGTGGTTGTTGTTGG+TGG	+	chr4.3:83514248-83514267	None:intergenic	50.0%
!	TGATGGGTCTTGTGATGCTC+CGG	+	chr4.3:83514162-83514181	None:intergenic	50.0%
!	TGGTTGTTGTTGGTGGAGGA+CGG	+	chr4.3:83514241-83514260	None:intergenic	50.0%
!!	GTTGAGAAGCTCTAGGAGAG+GGG	+	chr4.3:83514547-83514566	None:intergenic	50.0%
!!!	GTTTTTTAGAGCGGCGTTCG+TGG	+	chr4.3:83514307-83514326	None:intergenic	50.0%
	GCTTGGGCTGTTAGACGAGA+AGG	+	chr4.3:83514209-83514228	None:intergenic	55.0%
!	AGACGAGAAGGGAGTGTGAG+TGG	+	chr4.3:83514197-83514216	None:intergenic	55.0%
!	TGTTGTTGGTGGAGGACGGT+GGG	+	chr4.3:83514237-83514256	None:intergenic	55.0%
!	TTGTTGTTGGTGGAGGACGG+TGG	+	chr4.3:83514238-83514257	None:intergenic	55.0%
!	GCCCATTGGTGGTTCCATGG+TGG	+	chr4.3:83514261-83514280	None:intergenic	60.0%
!	AGGACGGTGGGCTTGGGCTT+GGG	+	chr4.3:83514225-83514244	None:intergenic	65.0%
!	TGGTGGAGGACGGTGGGCTT+GGG	+	chr4.3:83514231-83514250	None:intergenic	65.0%
!	TTGGTGGAGGACGGTGGGCT+TGG	+	chr4.3:83514232-83514251	None:intergenic	65.0%
!	GAGGACGGTGGGCTTGGGCT+TGG	+	chr4.3:83514226-83514245	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	83514059	83514631	83514059	ID=MS.gene060838
chr4.3	mRNA	83514059	83514631	83514059	ID=MS.gene060838.t1;Parent=MS.gene060838
chr4.3	exon	83514059	83514631	83514059	ID=MS.gene060838.t1.exon1;Parent=MS.gene060838.t1
chr4.3	CDS	83514059	83514631	83514059	ID=cds.MS.gene060838.t1;Parent=MS.gene060838.t1

Gene Sequence

>MS.gene060838

ATGCATTCACCAACATATGGATCCGACCTAACAACACAACCAAACACAACCTTTGTACAAGCAGACCCATCAAATTTCCGACAAGTTGTCCAAAAACTAACCGGAGCATCACAAGACCCATCAGCCCAAAAACTTCCACTCACACTCCCTTCTCGTCTAACAGCCCAAGCCCAAGCCCACCGTCCTCCACCAACAACAACCACCATGGAACCACCAATGGGCTTAAAAAAACCCAACTTCAAACTCCACGAACGCCGCTCTAAAAAACTCCAACTCAACGTTTCGGAAAATCCCATGATGTTCCAAAACAACAACAATAACATGATGATGATGTTAATGAATAGTAACAACAACAACAACATGCGAAGTGTTATTATGGCTTCACCAGTTTCACCATTGGAATATTACTATAGTCCAAAATCACCACATGAAAATGAAGAAAAAGAAGAAGAAAAAGTGATAGCTGAAAAAGGTTTCTATTTGCACCCCTCTCCTAGAGCTTCTCAACAACAACCACCTGAACTTTTACCTCTTTTTCCTTTGCATTCTCCTTCCTCTCAATCTCATTCTTAA

Protein sequence

>MS.gene060838.t1

MHSPTYGSDLTTQPNTTFVQADPSNFRQVVQKLTGASQDPSAQKLPLTLPSRLTAQAQAHRPPPTTTTMEPPMGLKKPNFKLHERRSKKLQLNVSENPMMFQNNNNNMMMMLMNSNNNNNMRSVIMASPVSPLEYYYSPKSPHENEEKEEEKVIAEKGFYLHPSPRASQQQPPELLPLFPLHSPSSQSHS