AlfalfaGEDB | MS.gene61206

M. sativa cultivar XinJiangDaYe / MS.gene61206

KEGG: -
GO: -
PFAM: PF04844.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61206.t1	XP_003597774.1	96.8	311	5	2	1	306	1	311	8.80E-121	443.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61206.t1	Q9S7T5	41.6	303	119	6	1	278	1	270	6.8e-29	129.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61206.t1	G7II51	96.8	311	5	2	1	306	1	311	6.4e-121	443.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene61206.t1	TF	OFP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049378	MS.gene61206	0.817633	2.98E-52	-1.69E-46
MS.gene051210	MS.gene61206	0.804652	1.95E-49	-1.69E-46
MS.gene052389	MS.gene61206	0.80165	8.15E-49	-1.69E-46
MS.gene054468	MS.gene61206	0.806065	9.86E-50	-1.69E-46
MS.gene054826	MS.gene61206	0.806735	7.12E-50	-1.69E-46
MS.gene055701	MS.gene61206	0.819798	9.61E-53	-1.69E-46
MS.gene057194	MS.gene61206	0.804317	2.29E-49	-1.69E-46
MS.gene058081	MS.gene61206	0.803053	4.19E-49	-1.69E-46
MS.gene059952	MS.gene61206	0.807827	4.18E-50	-1.69E-46
MS.gene06052	MS.gene61206	0.806662	7.38E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61206.t1	MTR_2g102140	97.749	311	2	2	1	306	1	311	0.0	617
MS.gene61206.t1	MTR_4g012060	47.634	317	128	9	1	306	1	290	4.28e-76	234
MS.gene61206.t1	MTR_1g072110	38.406	138	61	4	137	268	115	234	2.20e-18	82.8
MS.gene61206.t1	MTR_7g095150	47.887	71	35	1	198	268	132	200	4.63e-15	73.2
MS.gene61206.t1	MTR_1g111640	28.279	244	97	9	29	265	26	198	6.16e-13	67.8
MS.gene61206.t1	MTR_1g103640	42.188	64	34	1	203	266	117	177	4.99e-12	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61206.t1	AT1G79960	37.627	295	126	8	1	270	1	262	1.54e-47	161
MS.gene61206.t1	AT5G04820	35.338	133	62	4	134	266	102	210	3.10e-14	71.6
MS.gene61206.t1	AT4G14860	45.312	64	28	2	204	265	109	167	1.69e-11	62.4

Find 50 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGAGTCCTTACGACGATTTC+AGG	0.179517	2.1:+3257547	MS.gene61206:CDS
AAGCAGGTTACCATAGAATT+TGG	0.233067	2.1:+3257822	MS.gene61206:CDS
GAAAATTGATTGGGACTTCA+TGG	0.309098	2.1:+3257626	MS.gene61206:CDS
TTTGCAGCCAGAGAGCATTT+TGG	0.332403	2.1:-3257016	None:intergenic
CAGGAACCACCAATACGTTT+TGG	0.387081	2.1:-3257258	None:intergenic
ACAATCAAAGGAAAATTGAT+TGG	0.389239	2.1:+3257616	MS.gene61206:CDS
CTAAGAGGAACCAAATTCTA+TGG	0.390296	2.1:-3257832	None:intergenic
GTTAATCGAATTTGAGGCTG+TGG	0.438345	2.1:-3256994	None:intergenic
TATTGGTGGTTCCTGGTTGT+TGG	0.439269	2.1:+3257266	MS.gene61206:CDS
TTAGGATTGGTAGGGAAGTC+AGG	0.440786	2.1:+3257787	MS.gene61206:CDS
CAAAACGTATTGGTGGTTCC+TGG	0.454127	2.1:+3257259	MS.gene61206:CDS
GAGTTGGTGGAATCAAAGTA+TGG	0.465659	2.1:+3257582	MS.gene61206:CDS
GAAGAATTCAGAGGCTGCTC+CGG	0.469764	2.1:+3257740	MS.gene61206:CDS
CGATTAACCAAAATGCTCTC+TGG	0.470779	2.1:+3257009	MS.gene61206:CDS
CGTGTAGCGTGAGAACTGTT+AGG	0.472831	2.1:+3257769	MS.gene61206:CDS
AACAGTGCAACACAATTCTC+AGG	0.479484	2.1:-3257513	None:intergenic
AGGTAACTCAGGATCATTGA+TGG	0.483746	2.1:+3257356	MS.gene61206:CDS
ACACCACCTCAAGATCTTTG+TGG	0.490451	2.1:+3257309	MS.gene61206:CDS
CAATCAAAGGAAAATTGATT+GGG	0.491479	2.1:+3257617	MS.gene61206:CDS
TGAAATTCTCAAAGAGAAAA+CGG	0.493044	2.1:-3257143	None:intergenic
ATCAATGATCCTGAGTTACC+TGG	0.499280	2.1:-3257354	None:intergenic
AACAGCATCACCTTCAAAAG+AGG	0.511692	2.1:+3257449	MS.gene61206:CDS
TGAGAACTGTTAGGATTGGT+AGG	0.512241	2.1:+3257778	MS.gene61206:CDS
GCGATCAATGCAAGAGTTGG+TGG	0.513161	2.1:+3257569	MS.gene61206:CDS
CTGAAATCGTCGTAAGGACT+CGG	0.524949	2.1:-3257546	None:intergenic
TTTGTTGAGGTAATCTTGAA+GGG	0.525082	2.1:-3256956	None:intergenic
CAGGCGATCAATGCAAGAGT+TGG	0.529425	2.1:+3257566	MS.gene61206:CDS
TTGATCTGATCACTGTTATG+AGG	0.534780	2.1:+3257718	MS.gene61206:CDS
AAATTCTATGGTAACCTGCT+TGG	0.538786	2.1:-3257820	None:intergenic
CTCACGCTACACGGCTTCGC+CGG	0.538841	2.1:-3257759	None:intergenic
TTTGTTGTTAACGGCGTTTG+AGG	0.539359	2.1:-3257088	None:intergenic
GAGGAACCACCAAAACGTAT+TGG	0.549662	2.1:+3257249	MS.gene61206:CDS
GCACGATTCTTCGTGAAACC+AGG	0.558059	2.1:+3257336	MS.gene61206:CDS
TATGTTTCTGCCGTTATCCA+AGG	0.558329	2.1:-3257061	None:intergenic
CTAACAGTTCTCACGCTACA+CGG	0.576486	2.1:-3257768	None:intergenic
TTAATCGAATTTGAGGCTGT+GGG	0.576513	2.1:-3256993	None:intergenic
TTCGTGAAACCAGGTAACTC+AGG	0.588008	2.1:+3257345	MS.gene61206:CDS
AGCGTGAGAACTGTTAGGAT+TGG	0.588361	2.1:+3257774	MS.gene61206:CDS
CAGGAAGAAGAAGACCAAGC+AGG	0.606262	2.1:+3257806	MS.gene61206:CDS
TCTCGGTGACTCCAACAACC+AGG	0.608261	2.1:-3257277	None:intergenic
GTAAGATTGAAAACAATCAA+AGG	0.608557	2.1:+3257604	MS.gene61206:CDS
GAACCACCAAAACGTATTGG+TGG	0.614019	2.1:+3257252	MS.gene61206:CDS
GCTGAGCCACAAAGATCTTG+AGG	0.618030	2.1:-3257315	None:intergenic
TGTGATGATTGTGATCATCA+TGG	0.625055	2.1:-3257485	None:intergenic
GATCGCCTGAAATCGTCGTA+AGG	0.629682	2.1:-3257552	None:intergenic
GAGAACTGTTAGGATTGGTA+GGG	0.631326	2.1:+3257779	MS.gene61206:CDS
GGTGGTGTTGTAATGAATCT+CGG	0.636568	2.1:-3257294	None:intergenic
AATTGATTGGGACTTCATGG+AGG	0.677814	2.1:+3257629	MS.gene61206:CDS
TGTTATGAGGAAGAATTCAG+AGG	0.682074	2.1:+3257731	MS.gene61206:CDS
GAGCCACAAAGATCTTGAGG+TGG	0.753269	2.1:-3257312	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATCATTGATTTTGTTGTTAA+CGG	-	chr2.1:3257100-3257119	None:intergenic	20.0%
!!!	TTTTGTTTTTGATTTTGTTG+AGG	-	chr2.1:3256972-3256991	None:intergenic	20.0%
!	ACAATCAAAGGAAAATTGAT+TGG	+	chr2.1:3257616-3257635	MS.gene61206:CDS	25.0%
!	CAATCAAAGGAAAATTGATT+GGG	+	chr2.1:3257617-3257636	MS.gene61206:CDS	25.0%
!	GAACAAAAACAAAAGAATCT+CGG	+	chr2.1:3257212-3257231	MS.gene61206:CDS	25.0%
!	GTAAGATTGAAAACAATCAA+AGG	+	chr2.1:3257604-3257623	MS.gene61206:CDS	25.0%
!	TGAAATTCTCAAAGAGAAAA+CGG	-	chr2.1:3257146-3257165	None:intergenic	25.0%
!!!	ATTTTGGTTAATCGAATTTG+AGG	-	chr2.1:3257003-3257022	None:intergenic	25.0%
	TTTGTTGAGGTAATCTTGAA+GGG	-	chr2.1:3256959-3256978	None:intergenic	30.0%
!	AAGGAAAAAGATGGTGTTTT+GGG	-	chr2.1:3257045-3257064	None:intergenic	30.0%
!	ACTACAATAACCTCTTTTGA+AGG	-	chr2.1:3257462-3257481	None:intergenic	30.0%
!	TTTTGTTGAGGTAATCTTGA+AGG	-	chr2.1:3256960-3256979	None:intergenic	30.0%
	AAATTCTATGGTAACCTGCT+TGG	-	chr2.1:3257823-3257842	None:intergenic	35.0%
	AAGCAGGTTACCATAGAATT+TGG	+	chr2.1:3257822-3257841	MS.gene61206:CDS	35.0%
	CTAAGAGGAACCAAATTCTA+TGG	-	chr2.1:3257835-3257854	None:intergenic	35.0%
	GAAAATTGATTGGGACTTCA+TGG	+	chr2.1:3257626-3257645	MS.gene61206:CDS	35.0%
	TGTGATGATTGTGATCATCA+TGG	-	chr2.1:3257488-3257507	None:intergenic	35.0%
	TGTTATGAGGAAGAATTCAG+AGG	+	chr2.1:3257731-3257750	MS.gene61206:CDS	35.0%
	TTGATCTGATCACTGTTATG+AGG	+	chr2.1:3257718-3257737	MS.gene61206:CDS	35.0%
!	CAAAACACCATCTTTTTCCT+TGG	+	chr2.1:3257044-3257063	MS.gene61206:CDS	35.0%
!	CAAGGAAAAAGATGGTGTTT+TGG	-	chr2.1:3257046-3257065	None:intergenic	35.0%
!	CCATCTTTTTCCTTGGATAA+CGG	+	chr2.1:3257051-3257070	MS.gene61206:CDS	35.0%
!	TTAATCGAATTTGAGGCTGT+GGG	-	chr2.1:3256996-3257015	None:intergenic	35.0%
!!!	AGGGTTTTTTGAAGCTTCTT+AGG	-	chr2.1:3256940-3256959	None:intergenic	35.0%
	AACAGCATCACCTTCAAAAG+AGG	+	chr2.1:3257449-3257468	MS.gene61206:CDS	40.0%
	AACAGTGCAACACAATTCTC+AGG	-	chr2.1:3257516-3257535	None:intergenic	40.0%
	AATTGATTGGGACTTCATGG+AGG	+	chr2.1:3257629-3257648	MS.gene61206:CDS	40.0%
	AGGTAACTCAGGATCATTGA+TGG	+	chr2.1:3257356-3257375	MS.gene61206:CDS	40.0%
	ATCAATGATCCTGAGTTACC+TGG	-	chr2.1:3257357-3257376	None:intergenic	40.0%
	CCGTTATCCAAGGAAAAAGA+TGG	-	chr2.1:3257054-3257073	None:intergenic	40.0%
	CGATTAACCAAAATGCTCTC+TGG	+	chr2.1:3257009-3257028	MS.gene61206:CDS	40.0%
	CTCGGATGAAAAAAACAACG+AGG	+	chr2.1:3257230-3257249	MS.gene61206:CDS	40.0%
!	GAGAACTGTTAGGATTGGTA+GGG	+	chr2.1:3257779-3257798	MS.gene61206:CDS	40.0%
!	GTTAATCGAATTTGAGGCTG+TGG	-	chr2.1:3256997-3257016	None:intergenic	40.0%
!	TATGTTTCTGCCGTTATCCA+AGG	-	chr2.1:3257064-3257083	None:intergenic	40.0%
!	TGAGAACTGTTAGGATTGGT+AGG	+	chr2.1:3257778-3257797	MS.gene61206:CDS	40.0%
!!	GAGTTGGTGGAATCAAAGTA+TGG	+	chr2.1:3257582-3257601	MS.gene61206:CDS	40.0%
!!	GGTGGTGTTGTAATGAATCT+CGG	-	chr2.1:3257297-3257316	None:intergenic	40.0%
!!	TTTGTTGTTAACGGCGTTTG+AGG	-	chr2.1:3257091-3257110	None:intergenic	40.0%
	ACACCACCTCAAGATCTTTG+TGG	+	chr2.1:3257309-3257328	MS.gene61206:CDS	45.0%
	AGCGTGAGAACTGTTAGGAT+TGG	+	chr2.1:3257774-3257793	MS.gene61206:CDS	45.0%
	CTAACAGTTCTCACGCTACA+CGG	-	chr2.1:3257771-3257790	None:intergenic	45.0%
	CTGAAATCGTCGTAAGGACT+CGG	-	chr2.1:3257549-3257568	None:intergenic	45.0%
	GAACCACCAAAACGTATTGG+TGG	+	chr2.1:3257252-3257271	MS.gene61206:CDS	45.0%
	GAGGAACCACCAAAACGTAT+TGG	+	chr2.1:3257249-3257268	MS.gene61206:CDS	45.0%
	TTCGTGAAACCAGGTAACTC+AGG	+	chr2.1:3257345-3257364	MS.gene61206:CDS	45.0%
!	CAAAACGTATTGGTGGTTCC+TGG	+	chr2.1:3257259-3257278	MS.gene61206:CDS	45.0%
!	CAGGAACCACCAATACGTTT+TGG	-	chr2.1:3257261-3257280	None:intergenic	45.0%
!	GAACCACCAATACGTTTTGG+TGG	-	chr2.1:3257258-3257277	None:intergenic	45.0%
!	TATTGGTGGTTCCTGGTTGT+TGG	+	chr2.1:3257266-3257285	MS.gene61206:CDS	45.0%
!	TTAGGATTGGTAGGGAAGTC+AGG	+	chr2.1:3257787-3257806	MS.gene61206:CDS	45.0%
!!!	TTTGCAGCCAGAGAGCATTT+TGG	-	chr2.1:3257019-3257038	None:intergenic	45.0%
	CAGGAAGAAGAAGACCAAGC+AGG	+	chr2.1:3257806-3257825	MS.gene61206:CDS	50.0%
	CAGGCGATCAATGCAAGAGT+TGG	+	chr2.1:3257566-3257585	MS.gene61206:CDS	50.0%
	CGAGTCCTTACGACGATTTC+AGG	+	chr2.1:3257547-3257566	MS.gene61206:CDS	50.0%
	CGTGTAGCGTGAGAACTGTT+AGG	+	chr2.1:3257769-3257788	MS.gene61206:CDS	50.0%
	GAAGAATTCAGAGGCTGCTC+CGG	+	chr2.1:3257740-3257759	MS.gene61206:CDS	50.0%
	GAGCCACAAAGATCTTGAGG+TGG	-	chr2.1:3257315-3257334	None:intergenic	50.0%
	GATCGCCTGAAATCGTCGTA+AGG	-	chr2.1:3257555-3257574	None:intergenic	50.0%
	GCACGATTCTTCGTGAAACC+AGG	+	chr2.1:3257336-3257355	MS.gene61206:CDS	50.0%
	GCGATCAATGCAAGAGTTGG+TGG	+	chr2.1:3257569-3257588	MS.gene61206:CDS	50.0%
	GCTGAGCCACAAAGATCTTG+AGG	-	chr2.1:3257318-3257337	None:intergenic	50.0%
	TCTCGGTGACTCCAACAACC+AGG	-	chr2.1:3257280-3257299	None:intergenic	55.0%
	CTCACGCTACACGGCTTCGC+CGG	-	chr2.1:3257762-3257781	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	3256934	3257854	3256934	ID=MS.gene61206
chr2.1	mRNA	3256934	3257854	3256934	ID=MS.gene61206.t1;Parent=MS.gene61206
chr2.1	exon	3256934	3257854	3256934	ID=MS.gene61206.t1.exon1;Parent=MS.gene61206.t1
chr2.1	CDS	3256934	3257854	3256934	ID=cds.MS.gene61206.t1;Parent=MS.gene61206.t1

Gene Sequence

>MS.gene61206

ATGCCTAAGAAGCTTCAAAAAACCCTTCAAGATTACCTCAACAAAATCAAAAACAAAAACCCACAGCCTCAAATTCGATTAACCAAAATGCTCTCTGGCTGCAAACATCCCAAAACACCATCTTTTTCCTTGGATAACGGCAGAAACATATCATCCTCAAACGCCGTTAACAACAAAATCAATGATGCTGCAACACTAGCTGACGTTGACCGTTTTCTCTTTGAGAATTTCAAGTCTCTATATTTCAAAGATGATGAAGAAACAGAACAAAACAACAAGAACAAAAACAAAAGAATCTCGGATGAAAAAAACAACGAGGAACCACCAAAACGTATTGGTGGTTCCTGGTTGTTGGAGTCACCGAGATTCATTACAACACCACCTCAAGATCTTTGTGGCTCAGCACGATTCTTCGTGAAACCAGGTAACTCAGGATCATTGATGGAAGATGCACTGTCTCTAACAAACAGTGATGAAGCTGATTCGAGTAATTCGAATAATTCAAATTCATCATCAACAGCATCACCTTCAAAAGAGGTTATTGTAGTAAACCATGATGATCACAATCATCACACTCTTCCTGAGAATTGTGTTGCACTGTTATCATATTCTCCGAGTCCTTACGACGATTTCAGGCGATCAATGCAAGAGTTGGTGGAATCAAAGTATGGTAAGATTGAAAACAATCAAAGGAAAATTGATTGGGACTTCATGGAGGAGATTTTGTTTTGTTATCTTAATGTTAATGAGAAAAAATCTCATAAGTTTATATTAAGTGCTTTTGTTGATCTGATCACTGTTATGAGGAAGAATTCAGAGGCTGCTCCGGCGAAGCCGTGTAGCGTGAGAACTGTTAGGATTGGTAGGGAAGTCAGGAAGAAGAAGACCAAGCAGGTTACCATAGAATTTGGTTCCTCTTAG

Protein sequence

>MS.gene61206.t1

MPKKLQKTLQDYLNKIKNKNPQPQIRLTKMLSGCKHPKTPSFSLDNGRNISSSNAVNNKINDAATLADVDRFLFENFKSLYFKDDEETEQNNKNKNKRISDEKNNEEPPKRIGGSWLLESPRFITTPPQDLCGSARFFVKPGNSGSLMEDALSLTNSDEADSSNSNNSNSSSTASPSKEVIVVNHDDHNHHTLPENCVALLSYSPSPYDDFRRSMQELVESKYGKIENNQRKIDWDFMEEILFCYLNVNEKKSHKFILSAFVDLITVMRKNSEAAPAKPCSVRTVRIGREVRKKKTKQVTIEFGSS