Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61533.t1 | XP_003597762.1 | 90.1 | 121 | 5 | 3 | 1 | 119 | 88 | 203 | 6.90E-45 | 189.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61533.t1 | G7II32 | 90.1 | 121 | 5 | 3 | 1 | 119 | 88 | 203 | 4.9e-45 | 189.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene61533 | 0.819274 | 1.27E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene61533 | 0.8636 | 2.22E-64 | -1.69E-46 |
| MS.gene050808 | MS.gene61533 | 0.819716 | 1.00E-52 | -1.69E-46 |
| MS.gene050815 | MS.gene61533 | 0.8227 | 2.06E-53 | -1.69E-46 |
| MS.gene051180 | MS.gene61533 | 0.811497 | 6.80E-51 | -1.69E-46 |
| MS.gene051328 | MS.gene61533 | 0.817569 | 3.08E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene61533 | 0.819873 | 9.24E-53 | -1.69E-46 |
| MS.gene052665 | MS.gene61533 | 0.829895 | 3.99E-55 | -1.69E-46 |
| MS.gene053074 | MS.gene61533 | 0.804721 | 1.89E-49 | -1.69E-46 |
| MS.gene055465 | MS.gene61533 | 0.813239 | 2.83E-51 | -1.69E-46 |
| MS.gene055466 | MS.gene61533 | 0.829122 | 6.14E-55 | -1.69E-46 |
| MS.gene055468 | MS.gene61533 | 0.814144 | 1.79E-51 | -1.69E-46 |
| MS.gene055608 | MS.gene61533 | 0.824781 | 6.70E-54 | -1.69E-46 |
| MS.gene058168 | MS.gene61533 | 0.828756 | 7.53E-55 | -1.69E-46 |
| MS.gene058906 | MS.gene61533 | 0.802415 | 5.67E-49 | -1.69E-46 |
| MS.gene059937 | MS.gene61533 | 0.803161 | 3.98E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene61533 | 0.807792 | 4.25E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61533.t1 | MTR_2g101980 | 83.471 | 121 | 13 | 3 | 1 | 119 | 88 | 203 | 2.75e-59 | 181 |
| MS.gene61533.t1 | MTR_0147s0050 | 52.419 | 124 | 51 | 4 | 1 | 119 | 82 | 202 | 7.05e-33 | 114 |
| MS.gene61533.t1 | MTR_0147s0020 | 68.182 | 66 | 21 | 0 | 39 | 104 | 2 | 67 | 1.84e-27 | 99.0 |
| MS.gene61533.t1 | MTR_8g103530 | 50.000 | 72 | 32 | 2 | 48 | 118 | 134 | 202 | 4.12e-16 | 70.9 |
| MS.gene61533.t1 | MTR_6g086915 | 56.250 | 48 | 21 | 0 | 48 | 95 | 121 | 168 | 7.67e-14 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61533.t1 | AT1G15260 | 44.737 | 114 | 48 | 5 | 6 | 119 | 80 | 178 | 1.52e-19 | 79.3 |
| MS.gene61533.t1 | AT3G16070 | 63.830 | 47 | 17 | 0 | 48 | 94 | 104 | 150 | 4.87e-18 | 74.7 |
Find 11 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCACAATATCTAAATAAAT+AGG | 0.347318 | 2.4:-2927544 | None:intergenic |
| GAATTAGAGATGAATGATTC+AGG | 0.387057 | 2.4:+2927460 | MS.gene61533:CDS |
| GAATGAAGGTGTTAATGAAT+TGG | 0.390905 | 2.4:+2927423 | MS.gene61533:CDS |
| TCTAAGTCAAAGGGAAGATT+TGG | 0.402476 | 2.4:+2927631 | MS.gene61533:CDS |
| AGGGAAGATTTGGATCAATC+AGG | 0.432753 | 2.4:+2927641 | MS.gene61533:CDS |
| TCCTATTTATTTAGATATTG+TGG | 0.439985 | 2.4:+2927543 | MS.gene61533:CDS |
| TGAATTGGCATTGAAGATGA+AGG | 0.456133 | 2.4:+2927438 | MS.gene61533:CDS |
| AAAGGGAATAAGAAGAATGA+AGG | 0.496505 | 2.4:+2927409 | MS.gene61533:CDS |
| ATCAAACGCTCTAAGTCAAA+GGG | 0.509484 | 2.4:+2927622 | MS.gene61533:CDS |
| CATCAAACGCTCTAAGTCAA+AGG | 0.517132 | 2.4:+2927621 | MS.gene61533:CDS |
| AGAGCGTTTGATGCTGACAG+AGG | 0.645792 | 2.4:-2927611 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCCACAATATCTAAATAAAT+AGG | - | chr2.4:2927547-2927566 | None:intergenic | 20.0% |
| !! | TCCTATTTATTTAGATATTG+TGG | + | chr2.4:2927543-2927562 | MS.gene61533:CDS | 20.0% |
| ! | CAAAAAAACCTTACTGAAAA+TGG | + | chr2.4:2927367-2927386 | MS.gene61533:CDS | 25.0% |
| !!! | TTTTCAGTAAGGTTTTTTTG+TGG | - | chr2.4:2927367-2927386 | None:intergenic | 25.0% |
| AAAGGGAATAAGAAGAATGA+AGG | + | chr2.4:2927409-2927428 | MS.gene61533:CDS | 30.0% | |
| AGAGAGAGAAAAAAGACAAA+GGG | + | chr2.4:2927392-2927411 | MS.gene61533:CDS | 30.0% | |
| GAATTAGAGATGAATGATTC+AGG | + | chr2.4:2927460-2927479 | MS.gene61533:CDS | 30.0% | |
| !! | GAATGAAGGTGTTAATGAAT+TGG | + | chr2.4:2927423-2927442 | MS.gene61533:CDS | 30.0% |
| GAGAGAGAGAAAAAAGACAA+AGG | + | chr2.4:2927391-2927410 | MS.gene61533:CDS | 35.0% | |
| TCTAAGTCAAAGGGAAGATT+TGG | + | chr2.4:2927631-2927650 | MS.gene61533:CDS | 35.0% | |
| ! | ATCAAACGCTCTAAGTCAAA+GGG | + | chr2.4:2927622-2927641 | MS.gene61533:CDS | 35.0% |
| ! | TGAATTGGCATTGAAGATGA+AGG | + | chr2.4:2927438-2927457 | MS.gene61533:CDS | 35.0% |
| AGGGAAGATTTGGATCAATC+AGG | + | chr2.4:2927641-2927660 | MS.gene61533:CDS | 40.0% | |
| ! | CATCAAACGCTCTAAGTCAA+AGG | + | chr2.4:2927621-2927640 | MS.gene61533:CDS | 40.0% |
| ! | CTCTCTCTCCATTTTCAGTA+AGG | - | chr2.4:2927378-2927397 | None:intergenic | 40.0% |
| ! | AGAGCGTTTGATGCTGACAG+AGG | - | chr2.4:2927614-2927633 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 2927310 | 2927669 | 2927310 | ID=MS.gene61533 |
| chr2.4 | mRNA | 2927310 | 2927669 | 2927310 | ID=MS.gene61533.t1;Parent=MS.gene61533 |
| chr2.4 | exon | 2927310 | 2927669 | 2927310 | ID=MS.gene61533.t1.exon1;Parent=MS.gene61533.t1 |
| chr2.4 | CDS | 2927310 | 2927669 | 2927310 | ID=cds.MS.gene61533.t1;Parent=MS.gene61533.t1 |
| Gene Sequence |
| Protein sequence |
