AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62681

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62681.t1 MTR_1g115415 97.964 442 9 0 1 442 60 501 0.0 869
MS.gene62681.t1 MTR_8g105560 82.955 440 22 2 1 440 38 424 0.0 705
MS.gene62681.t1 MTR_5g068860 53.250 400 185 1 12 409 103 502 2.05e-152 443
MS.gene62681.t1 MTR_4g070060 25.000 360 260 4 45 397 235 591 1.22e-34 136
MS.gene62681.t1 MTR_6g003960 27.164 335 238 6 65 397 263 593 4.17e-27 114
MS.gene62681.t1 MTR_2g034600 27.972 286 162 5 149 396 208 487 1.41e-26 112
MS.gene62681.t1 MTR_2g034600 22.348 264 179 5 8 267 189 430 4.76e-12 68.2
MS.gene62681.t1 MTR_2g034600 27.972 286 162 5 149 396 273 552 1.59e-26 112
MS.gene62681.t1 MTR_2g034600 22.348 264 179 5 8 267 254 495 4.04e-12 68.6
MS.gene62681.t1 MTR_2g034600 27.972 286 162 5 149 396 269 548 1.62e-26 112
MS.gene62681.t1 MTR_2g034600 22.348 264 179 5 8 267 250 491 4.63e-12 68.2
MS.gene62681.t1 MTR_4g084630 22.436 312 198 5 86 392 47 319 7.16e-26 107
MS.gene62681.t1 MTR_4g084630 25.108 231 147 3 23 252 45 250 2.83e-17 82.8
MS.gene62681.t1 MTR_5g094610 24.373 279 200 7 102 373 176 450 1.43e-19 91.3
MS.gene62681.t1 MTR_5g094610 24.627 268 186 8 22 277 194 457 5.71e-16 80.5
MS.gene62681.t1 MTR_2g049780 26.923 208 116 3 222 393 99 306 5.14e-16 79.0
MS.gene62681.t1 MTR_5g041630 27.059 255 138 7 151 397 88 302 6.61e-14 72.8
MS.gene62681.t1 MTR_2g067230 23.659 317 151 11 86 397 75 305 1.75e-13 71.6
MS.gene62681.t1 MTR_3g092710 22.819 298 184 7 99 391 235 491 1.71e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62681.t1 AT2G44020 80.361 443 86 1 1 442 65 507 0.0 729
MS.gene62681.t1 AT4G02990 53.181 393 184 0 13 405 112 504 9.60e-153 444
MS.gene62681.t1 AT4G02990 53.181 393 184 0 13 405 112 504 9.60e-153 444
MS.gene62681.t1 AT2G21710 24.716 352 261 3 77 427 278 626 1.07e-38 148
MS.gene62681.t1 AT2G21710 26.070 257 176 5 7 252 272 525 2.29e-17 85.1
MS.gene62681.t1 AT1G78930 26.488 336 240 6 65 397 249 580 1.87e-31 127
MS.gene62681.t1 AT4G38160 25.547 274 195 4 128 395 54 324 8.19e-29 116
MS.gene62681.t1 AT4G38160 24.020 204 153 2 65 266 63 266 1.11e-15 78.2
MS.gene62681.t1 AT4G38160 25.547 274 195 4 128 395 54 324 8.19e-29 116
MS.gene62681.t1 AT4G38160 24.020 204 153 2 65 266 63 266 1.11e-15 78.2
MS.gene62681.t1 AT4G38160 25.547 274 195 4 128 395 54 324 1.43e-28 116
MS.gene62681.t1 AT4G38160 23.902 205 154 2 65 267 63 267 1.31e-15 78.6
MS.gene62681.t1 AT4G38160 25.547 274 195 4 128 395 54 324 1.53e-28 115
MS.gene62681.t1 AT4G38160 23.902 205 154 2 65 267 63 267 1.61e-15 78.2
MS.gene62681.t1 AT2G21710 23.571 280 212 2 77 355 278 556 1.13e-26 113
MS.gene62681.t1 AT2G21710 26.070 257 176 5 7 252 272 525 1.83e-17 85.1
MS.gene62681.t1 AT2G21710 23.381 278 211 2 77 353 278 554 8.79e-26 110
MS.gene62681.t1 AT2G21710 26.070 257 176 5 7 252 272 525 2.18e-17 85.1
MS.gene62681.t1 AT4G14605 25.130 386 268 9 14 392 97 468 7.34e-24 104
MS.gene62681.t1 AT4G14605 25.130 386 268 9 14 392 97 468 7.34e-24 104
MS.gene62681.t1 AT5G55580 26.484 219 154 4 158 371 119 335 2.71e-19 89.7
MS.gene62681.t1 AT5G55580 25.385 260 179 7 20 267 79 335 1.42e-16 81.6
MS.gene62681.t1 AT5G55580 23.853 218 160 3 84 299 117 330 2.11e-14 75.1
MS.gene62681.t1 AT5G55580 26.484 219 154 4 158 371 225 441 1.77e-18 88.2
MS.gene62681.t1 AT5G55580 25.385 260 179 7 20 267 185 441 2.11e-16 81.6
MS.gene62681.t1 AT5G55580 23.767 223 164 3 84 304 223 441 6.56e-14 73.9
MS.gene62681.t1 AT5G54180 23.311 296 184 7 101 393 235 490 3.94e-15 77.8
MS.gene62681.t1 AT1G61980 24.118 340 235 10 77 403 81 410 1.21e-14 75.9
MS.gene62681.t1 AT1G61980 24.118 340 235 10 77 403 81 410 1.21e-14 75.9
MS.gene62681.t1 AT1G61980 24.118 340 235 10 77 403 81 410 1.21e-14 75.9
MS.gene62681.t1 AT2G34620 26.887 212 119 4 222 397 85 296 1.81e-13 71.2
MS.gene62681.t1 AT2G03050 29.317 249 151 9 158 395 39 273 4.73e-13 69.7
MS.gene62681.t1 AT2G03050 29.317 249 151 9 158 395 38 272 4.90e-13 69.7
MS.gene62681.t1 AT5G55580 25.000 172 127 2 84 253 223 394 2.01e-12 68.9
MS.gene62681.t1 AT5G55580 26.943 193 129 5 20 200 185 377 2.82e-12 68.6
MS.gene62681.t1 AT2G36000 23.506 251 169 8 158 399 79 315 5.16e-12 67.0
MS.gene62681.t1 AT2G36000 23.387 248 167 8 158 396 79 312 1.07e-11 66.2

Find 96 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCAGTATCCTTTGATTT+TGG 0.073063 4.2:+1424738 MS.gene62681:CDS
GTTGAGTTTATTCAGAAATT+AGG 0.154219 4.2:+1424697 MS.gene62681:CDS
GTTGATTACTTGGTTAATTT+AGG 0.165369 4.2:+1425117 MS.gene62681:CDS
GCTCAGTATCCGCAGATTAT+TGG 0.227610 4.2:+1425279 MS.gene62681:CDS
TCCTGATGAATTGCAGGATT+TGG 0.241747 4.2:+1424639 MS.gene62681:CDS
TTGATTACTTGGTTAATTTA+GGG 0.248506 4.2:+1425118 MS.gene62681:CDS
GGATAAAATGTTCGTTGAAT+TGG 0.261081 4.2:+1425667 MS.gene62681:CDS
AGAATACTTCACTTATAGTT+TGG 0.295127 4.2:+1425602 MS.gene62681:CDS
CTCAGTATCCGCAGATTATT+GGG 0.297010 4.2:+1425280 MS.gene62681:CDS
CTTCGATTATCTCAAGGGTT+TGG 0.298946 4.2:+1424609 MS.gene62681:CDS
GTTGATTGTTTGATTAGTTT+TGG 0.307251 4.2:+1425225 MS.gene62681:CDS
CTCTTAAGCCTCTGGTATCT+AGG 0.309752 4.2:-1425639 None:intergenic
TTCGATTATCTCAAGGGTTT+GGG 0.314201 4.2:+1424610 MS.gene62681:CDS
TTGAGTTTATTCAGAAATTA+GGG 0.329984 4.2:+1424698 MS.gene62681:CDS
GATTATTGGGTTGCCTCTTA+AGG 0.337713 4.2:+1425293 MS.gene62681:CDS
CTTCAACCGTTGAAGTTATG+AGG 0.356463 4.2:+1424668 MS.gene62681:CDS
AATTATTCCTGATGAATTGC+AGG 0.358521 4.2:+1424633 MS.gene62681:CDS
CCTGTTATTAAGTTTCTTAG+AGG 0.361016 4.2:+1424895 MS.gene62681:CDS
AAACTATAAGTGAAGTATTC+TGG 0.362016 4.2:-1425600 None:intergenic
TCAAAATGTGATTATGAAAC+CGG 0.364444 4.2:+1425419 MS.gene62681:CDS
CTAATCAAACAATCAACATT+AGG 0.365413 4.2:-1425219 None:intergenic
CCTCTAAGAAACTTAATAAC+AGG 0.368936 4.2:-1424895 None:intergenic
TTAGTATTGGTGTTAATCCT+AGG 0.373470 4.2:+1425022 MS.gene62681:CDS
TCCCCTTGCTCTTAAGCCTC+TGG 0.375995 4.2:-1425647 None:intergenic
GAGATTTGAAGAGAGATTGC+AGG 0.401297 4.2:+1425710 MS.gene62681:CDS
GTCGCAGGATGTGGCTAGTA+TGG 0.403205 4.2:+1425470 MS.gene62681:CDS
GTAGCTTATCTTGTTAGTAT+TGG 0.406186 4.2:+1425009 MS.gene62681:CDS
CTCATAACTTCAACGGTTGA+AGG 0.413170 4.2:-1424667 None:intergenic
GAGAAAAGAGCTTATTTACT+TGG 0.423347 4.2:+1425171 MS.gene62681:CDS
ACCACCCTCGCAAACCCTTC+CGG 0.428125 4.2:-1425366 None:intergenic
GAAGACAATTTAGCCTTAAG+AGG 0.431995 4.2:-1425306 None:intergenic
GCAATCTCTCTTCAAATCTC+TGG 0.437449 4.2:-1425707 None:intergenic
GAATGAAGGACCTATACTTT+CGG 0.437668 4.2:-1425748 None:intergenic
TCCGGAAGGGTTTGCGAGGG+TGG 0.445823 4.2:+1425365 MS.gene62681:CDS
GGTGTTAATCCTAGGGATAT+TGG 0.451217 4.2:+1425030 MS.gene62681:CDS
TACTTCTTCAAAAGTGAGAT+GGG 0.459036 4.2:+1425549 MS.gene62681:CDS
GGAAGGGTTTGCGAGGGTGG+TGG 0.459551 4.2:+1425368 MS.gene62681:CDS
GAGGAAGGAATGTTTGCCTT+CGG 0.461475 4.2:+1425251 MS.gene62681:CDS
CGAGATGCCTACTGTTACAT+GGG 0.462699 4.2:+1424540 None:intergenic
TCCAAATCCTGCAATTCATC+AGG 0.465291 4.2:-1424640 None:intergenic
CATAGTTTCAGATGAGGAAG+AGG 0.486125 4.2:+1425806 MS.gene62681:CDS
ATCCTAGCTAGAATCTTCTT+AGG 0.491214 4.2:-1425144 None:intergenic
GGGATTTCTAGGTCTAAACT+TGG 0.492563 4.2:+1424814 MS.gene62681:CDS
ATAGGTCCTTCATTCCGTAT+TGG 0.501976 4.2:+1425756 MS.gene62681:CDS
TACCAGAGGCTTAAGAGCAA+GGG 0.502139 4.2:+1425645 MS.gene62681:CDS
CATCATCTTCGATTATCTCA+AGG 0.503798 4.2:+1424603 MS.gene62681:CDS
GGCAATACCGTCGCAGGATG+TGG 0.504137 4.2:+1425461 MS.gene62681:CDS
TTTGAAGCTTAAGATTGATC+CGG 0.506836 4.2:+1425347 MS.gene62681:CDS
TTACTTCTTCAAAAGTGAGA+TGG 0.508844 4.2:+1425548 MS.gene62681:CDS
TCCTAGGGATATTGGTCCAA+TGG 0.513309 4.2:+1425038 MS.gene62681:CDS
GTTACATGGGGTGTAATTCA+AGG 0.515509 4.2:+1424553 MS.gene62681:CDS
TTAACCAAGTAATCAACAAA+AGG 0.515918 4.2:-1425111 None:intergenic
GATGTGGAGAAAGATGATAT+TGG 0.522013 4.2:+1424922 MS.gene62681:CDS
TAGTATTGGTGTTAATCCTA+GGG 0.522188 4.2:+1425023 MS.gene62681:CDS
ACGAGATGCCTACTGTTACA+TGG 0.523419 4.2:+1424539 None:intergenic
TATATTGAAACCGAAAGTAT+AGG 0.527577 4.2:+1425738 MS.gene62681:CDS
TAAGGATACTGTGTAACCAT+TGG 0.531997 4.2:-1425054 None:intergenic
ATCATCTTCGATTATCTCAA+GGG 0.534022 4.2:+1424604 MS.gene62681:CDS
GCTCTAATTTCCCACCAATA+CGG 0.538425 4.2:-1425770 None:intergenic
GGTCCTTCATTCCGTATTGG+TGG 0.539928 4.2:+1425759 MS.gene62681:CDS
AGTTTAAACCCTAAAAGTTG+AGG 0.546911 4.2:-1424964 None:intergenic
TTCCCACCAATACGGAATGA+AGG 0.553754 4.2:-1425762 None:intergenic
ACTCGCAGCGCAATCAATTG+AGG 0.555993 4.2:-1425504 None:intergenic
AGCTTAAGATTGATCCGGAA+GGG 0.556415 4.2:+1425352 MS.gene62681:CDS
GTCCTTCATTCCGTATTGGT+GGG 0.557863 4.2:+1425760 MS.gene62681:CDS
TTCAACCGTTGAAGTTATGA+GGG 0.560167 4.2:+1424669 MS.gene62681:CDS
AGATTTGAAGAGAGATTGCA+GGG 0.561148 4.2:+1425711 MS.gene62681:CDS
ATACCAGAGGCTTAAGAGCA+AGG 0.565715 4.2:+1425644 MS.gene62681:CDS
CAAAGAGAAACTCGTTTCGC+GGG 0.570276 4.2:+1424579 MS.gene62681:CDS
GAAGATTCTAGCTAGGATGT+TGG 0.572863 4.2:+1425149 MS.gene62681:CDS
GCAAAGAGAAACTCGTTTCG+CGG 0.577091 4.2:+1424578 MS.gene62681:CDS
ACACTAGCATGCAAAACTTG+TGG 0.577739 4.2:-1424856 None:intergenic
TGGTAGGGCAATACCGTCGC+AGG 0.580954 4.2:+1425455 MS.gene62681:CDS
ACGTTCCCTCATAACTTCAA+CGG 0.581773 4.2:-1424674 None:intergenic
GAGGCCTGTAAAAGAGCTTG+TGG 0.585209 4.2:+1425572 MS.gene62681:CDS
ACACTACAGCCCAAAATCAA+AGG 0.590303 4.2:-1424748 None:intergenic
ACCAGAGGCTTAAGAGCAAG+GGG 0.605082 4.2:+1425646 MS.gene62681:CDS
TTGATCCGGAAGGGTTTGCG+AGG 0.614047 4.2:+1425361 MS.gene62681:CDS
TGCCTAAGAAGATTCTAGCT+AGG 0.620780 4.2:+1425142 MS.gene62681:CDS
AAGCTTAAGATTGATCCGGA+AGG 0.621599 4.2:+1425351 MS.gene62681:CDS
TCTTCAAAAGTGAGATGGGG+AGG 0.627180 4.2:+1425553 MS.gene62681:CDS
GTTTCTTAGAGGACTTGATG+TGG 0.627869 4.2:+1424906 MS.gene62681:CDS
GGTGGTGGAGAAGATGCCAC+AGG 0.628330 4.2:+1425383 MS.gene62681:CDS
ACCATTGGACCAATATCCCT+AGG 0.638044 4.2:-1425039 None:intergenic
ATTACACCCCATGTAACAGT+AGG 0.644138 4.2:-1424547 None:intergenic
ACTTCTTCAAAAGTGAGATG+GGG 0.645282 4.2:+1425550 MS.gene62681:CDS
AAACGACATAGTTTCAGATG+AGG 0.649241 4.2:+1425800 MS.gene62681:CDS
TGATGAAGACTAACCACCTG+TGG 0.651476 4.2:-1425399 None:intergenic
TGATCCGGAAGGGTTTGCGA+GGG 0.652425 4.2:+1425362 MS.gene62681:CDS
TGAAATGCTATACAGAAGAA+CGG 0.652624 4.2:+1425839 MS.gene62681:CDS
ATACTAGCCACATCCTGCGA+CGG 0.678965 4.2:-1425468 None:intergenic
GGTGGAGAAGATGCCACAGG+TGG 0.679410 4.2:+1425386 MS.gene62681:CDS
GGATACTGAGCAATAACCGA+AGG 0.689064 4.2:-1425267 None:intergenic
AGAGGCAACCCAATAATCTG+CGG 0.697237 4.2:-1425288 None:intergenic
GAATTAAACCTAGATACCAG+AGG 0.721224 4.2:+1425631 MS.gene62681:CDS
GAGATGCCTACTGTTACATG+GGG 0.725465 4.2:+1424541 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAACTGAAAAAATATTGTT+GGG - chr4.2:1425331-1425350 None:intergenic 20.0%
!! TCAAACTGAAAAAATATTGT+TGG - chr4.2:1425332-1425351 None:intergenic 20.0%
!! TTGAGTTTATTCAGAAATTA+GGG + chr4.2:1424698-1424717 MS.gene62681:CDS 20.0%
!! TTGATTACTTGGTTAATTTA+GGG + chr4.2:1425118-1425137 MS.gene62681:CDS 20.0%
!!! AAAAATATCCTCAACTTTTA+GGG + chr4.2:1424956-1424975 MS.gene62681:CDS 20.0%
!!! TAGGGTATTTAGAAAAAATT+GGG + chr4.2:1424794-1424813 MS.gene62681:CDS 20.0%
!!! TTAGGGTATTTAGAAAAAAT+TGG + chr4.2:1424793-1424812 MS.gene62681:CDS 20.0%
! AGAATACTTCACTTATAGTT+TGG + chr4.2:1425602-1425621 MS.gene62681:CDS 25.0%
! CTAATCAAACAATCAACATT+AGG - chr4.2:1425222-1425241 None:intergenic 25.0%
! GTTGAGTTTATTCAGAAATT+AGG + chr4.2:1424697-1424716 MS.gene62681:CDS 25.0%
! GTTGATTACTTGGTTAATTT+AGG + chr4.2:1425117-1425136 MS.gene62681:CDS 25.0%
! TAGAAAAAATTGGGATTTCT+AGG + chr4.2:1424803-1424822 MS.gene62681:CDS 25.0%
! TCAAAATGTGATTATGAAAC+CGG + chr4.2:1425419-1425438 MS.gene62681:CDS 25.0%
! TTAACCAAGTAATCAACAAA+AGG - chr4.2:1425114-1425133 None:intergenic 25.0%
!! AAAAACATGATTCCTGTTTT+AGG + chr4.2:1424775-1424794 MS.gene62681:CDS 25.0%
!! AAAACATGATTCCTGTTTTA+GGG + chr4.2:1424776-1424795 MS.gene62681:CDS 25.0%
!! AAACTATAAGTGAAGTATTC+TGG - chr4.2:1425603-1425622 None:intergenic 25.0%
!! CAAAAATATCCTCAACTTTT+AGG + chr4.2:1424955-1424974 MS.gene62681:CDS 25.0%
!! TATATTGAAACCGAAAGTAT+AGG + chr4.2:1425738-1425757 MS.gene62681:CDS 25.0%
!! TTTTCTAAATACCCTAAAAC+AGG - chr4.2:1424790-1424809 None:intergenic 25.0%
!!! AAATCAGTATCCTTTGATTT+TGG + chr4.2:1424738-1424757 MS.gene62681:CDS 25.0%
!!! AATCAGTATCCTTTGATTTT+GGG + chr4.2:1424739-1424758 MS.gene62681:CDS 25.0%
!!! ACACAGTATCCTTATTTTTT+AGG + chr4.2:1425063-1425082 MS.gene62681:CDS 25.0%
!!! CACAGTATCCTTATTTTTTA+GGG + chr4.2:1425064-1425083 MS.gene62681:CDS 25.0%
!!! GTTGATTGTTTGATTAGTTT+TGG + chr4.2:1425225-1425244 MS.gene62681:CDS 25.0%
AATTATTCCTGATGAATTGC+AGG + chr4.2:1424633-1424652 MS.gene62681:CDS 30.0%
ACTCTCATCCCTAAAAAATA+AGG - chr4.2:1425075-1425094 None:intergenic 30.0%
AGTTTAAACCCTAAAAGTTG+AGG - chr4.2:1424967-1424986 None:intergenic 30.0%
ATCATCTTCGATTATCTCAA+GGG + chr4.2:1424604-1424623 MS.gene62681:CDS 30.0%
CCTGTTATTAAGTTTCTTAG+AGG + chr4.2:1424895-1424914 MS.gene62681:CDS 30.0%
GAGAAAAGAGCTTATTTACT+TGG + chr4.2:1425171-1425190 MS.gene62681:CDS 30.0%
GGATAAAATGTTCGTTGAAT+TGG + chr4.2:1425667-1425686 MS.gene62681:CDS 30.0%
GTAGCTTATCTTGTTAGTAT+TGG + chr4.2:1425009-1425028 MS.gene62681:CDS 30.0%
TACTTCTTCAAAAGTGAGAT+GGG + chr4.2:1425549-1425568 MS.gene62681:CDS 30.0%
TGAAATGCTATACAGAAGAA+CGG + chr4.2:1425839-1425858 MS.gene62681:CDS 30.0%
TTACTTCTTCAAAAGTGAGA+TGG + chr4.2:1425548-1425567 MS.gene62681:CDS 30.0%
! CCTCTAAGAAACTTAATAAC+AGG - chr4.2:1424898-1424917 None:intergenic 30.0%
! TTTGAAGCTTAAGATTGATC+CGG + chr4.2:1425347-1425366 MS.gene62681:CDS 30.0%
!! AAAGCCTTTTGTTGATTACT+TGG + chr4.2:1425107-1425126 MS.gene62681:CDS 30.0%
!! TAGTATTGGTGTTAATCCTA+GGG + chr4.2:1425023-1425042 MS.gene62681:CDS 30.0%
!! TTAGTATTGGTGTTAATCCT+AGG + chr4.2:1425022-1425041 MS.gene62681:CDS 30.0%
!!! CTTTTAGGGTTTAAACTTGA+AGG + chr4.2:1424970-1424989 MS.gene62681:CDS 30.0%
!!! GTTTGATTAGTTTTGGTTTG+AGG + chr4.2:1425232-1425251 MS.gene62681:CDS 30.0%
!!! TATTTTTTAGGGATGAGAGT+TGG + chr4.2:1425075-1425094 MS.gene62681:CDS 30.0%
AAACGACATAGTTTCAGATG+AGG + chr4.2:1425800-1425819 MS.gene62681:CDS 35.0%
ACTTCTTCAAAAGTGAGATG+GGG + chr4.2:1425550-1425569 MS.gene62681:CDS 35.0%
AGATTTGAAGAGAGATTGCA+GGG + chr4.2:1425711-1425730 MS.gene62681:CDS 35.0%
ATCCTAGCTAGAATCTTCTT+AGG - chr4.2:1425147-1425166 None:intergenic 35.0%
CATCATCTTCGATTATCTCA+AGG + chr4.2:1424603-1424622 MS.gene62681:CDS 35.0%
CTACCAAGTAAAAACTCAAC+CGG - chr4.2:1425441-1425460 None:intergenic 35.0%
GAATGAAGGACCTATACTTT+CGG - chr4.2:1425751-1425770 None:intergenic 35.0%
GAATTAAACCTAGATACCAG+AGG + chr4.2:1425631-1425650 MS.gene62681:CDS 35.0%
GATGTGGAGAAAGATGATAT+TGG + chr4.2:1424922-1424941 MS.gene62681:CDS 35.0%
TAAGGATACTGTGTAACCAT+TGG - chr4.2:1425057-1425076 None:intergenic 35.0%
TTCAACCGTTGAAGTTATGA+GGG + chr4.2:1424669-1424688 MS.gene62681:CDS 35.0%
TTCGATTATCTCAAGGGTTT+GGG + chr4.2:1424610-1424629 MS.gene62681:CDS 35.0%
! AAACCGGTTGAGTTTTTACT+TGG + chr4.2:1425435-1425454 MS.gene62681:CDS 35.0%
! GGTTGAGTTTTTACTTGGTA+GGG + chr4.2:1425440-1425459 MS.gene62681:CDS 35.0%
!! GAAGACAATTTAGCCTTAAG+AGG - chr4.2:1425309-1425328 None:intergenic 35.0%
!!! GATTAGTTTTGGTTTGAGGA+AGG + chr4.2:1425236-1425255 MS.gene62681:CDS 35.0%
!!! TCTGAAACTATGTCGTTTTC+TGG - chr4.2:1425798-1425817 None:intergenic 35.0%
AAGCTTAAGATTGATCCGGA+AGG + chr4.2:1425351-1425370 MS.gene62681:CDS 40.0%
ACACTACAGCCCAAAATCAA+AGG - chr4.2:1424751-1424770 None:intergenic 40.0%
ACACTAGCATGCAAAACTTG+TGG - chr4.2:1424859-1424878 None:intergenic 40.0%
ACGTTCCCTCATAACTTCAA+CGG - chr4.2:1424677-1424696 None:intergenic 40.0%
AGCTTAAGATTGATCCGGAA+GGG + chr4.2:1425352-1425371 MS.gene62681:CDS 40.0%
ATAGGTCCTTCATTCCGTAT+TGG + chr4.2:1425756-1425775 MS.gene62681:CDS 40.0%
ATTACACCCCATGTAACAGT+AGG - chr4.2:1424550-1424569 None:intergenic 40.0%
CATAGTTTCAGATGAGGAAG+AGG + chr4.2:1425806-1425825 MS.gene62681:CDS 40.0%
CTCAGTATCCGCAGATTATT+GGG + chr4.2:1425280-1425299 MS.gene62681:CDS 40.0%
CTCATAACTTCAACGGTTGA+AGG - chr4.2:1424670-1424689 None:intergenic 40.0%
CTTCAACCGTTGAAGTTATG+AGG + chr4.2:1424668-1424687 MS.gene62681:CDS 40.0%
CTTCGATTATCTCAAGGGTT+TGG + chr4.2:1424609-1424628 MS.gene62681:CDS 40.0%
GAGATTTGAAGAGAGATTGC+AGG + chr4.2:1425710-1425729 MS.gene62681:CDS 40.0%
GCAATCTCTCTTCAAATCTC+TGG - chr4.2:1425710-1425729 None:intergenic 40.0%
GCTCTAATTTCCCACCAATA+CGG - chr4.2:1425773-1425792 None:intergenic 40.0%
GGTGTTAATCCTAGGGATAT+TGG + chr4.2:1425030-1425049 MS.gene62681:CDS 40.0%
GTTACATGGGGTGTAATTCA+AGG + chr4.2:1424553-1424572 MS.gene62681:CDS 40.0%
GTTTCTTAGAGGACTTGATG+TGG + chr4.2:1424906-1424925 MS.gene62681:CDS 40.0%
TCCAAATCCTGCAATTCATC+AGG - chr4.2:1424643-1424662 None:intergenic 40.0%
TCCTGATGAATTGCAGGATT+TGG + chr4.2:1424639-1424658 MS.gene62681:CDS 40.0%
! AACTCCACAAGCTCTTTTAC+AGG - chr4.2:1425579-1425598 None:intergenic 40.0%
! CGGTTGAGTTTTTACTTGGT+AGG + chr4.2:1425439-1425458 MS.gene62681:CDS 40.0%
! GAAGATTCTAGCTAGGATGT+TGG + chr4.2:1425149-1425168 MS.gene62681:CDS 40.0%
! GATTATTGGGTTGCCTCTTA+AGG + chr4.2:1425293-1425312 MS.gene62681:CDS 40.0%
! GGGATTTCTAGGTCTAAACT+TGG + chr4.2:1424814-1424833 MS.gene62681:CDS 40.0%
! TGATTTTGGGCTGTAGTGTT+CGG + chr4.2:1424752-1424771 MS.gene62681:CDS 40.0%
! TGCCTAAGAAGATTCTAGCT+AGG + chr4.2:1425142-1425161 MS.gene62681:CDS 40.0%
ACCATTGGACCAATATCCCT+AGG - chr4.2:1425042-1425061 None:intergenic 45.0%
AGAGGCAACCCAATAATCTG+CGG - chr4.2:1425291-1425310 None:intergenic 45.0%
ATACCAGAGGCTTAAGAGCA+AGG + chr4.2:1425644-1425663 MS.gene62681:CDS 45.0%
CAAAGAGAAACTCGTTTCGC+GGG + chr4.2:1424579-1424598 MS.gene62681:CDS 45.0%
CTCTTAAGCCTCTGGTATCT+AGG - chr4.2:1425642-1425661 None:intergenic 45.0%
GAGGAAGGAATGTTTGCCTT+CGG + chr4.2:1425251-1425270 MS.gene62681:CDS 45.0%
GCAAAGAGAAACTCGTTTCG+CGG + chr4.2:1424578-1424597 MS.gene62681:CDS 45.0%
GCTCAGTATCCGCAGATTAT+TGG + chr4.2:1425279-1425298 MS.gene62681:CDS 45.0%
GGATACTGAGCAATAACCGA+AGG - chr4.2:1425270-1425289 None:intergenic 45.0%
TACCAGAGGCTTAAGAGCAA+GGG + chr4.2:1425645-1425664 MS.gene62681:CDS 45.0%
TCCTAGGGATATTGGTCCAA+TGG + chr4.2:1425038-1425057 MS.gene62681:CDS 45.0%
TCTTCAAAAGTGAGATGGGG+AGG + chr4.2:1425553-1425572 MS.gene62681:CDS 45.0%
TGATGAAGACTAACCACCTG+TGG - chr4.2:1425402-1425421 None:intergenic 45.0%
TTCCCACCAATACGGAATGA+AGG - chr4.2:1425765-1425784 None:intergenic 45.0%
! GTCCTTCATTCCGTATTGGT+GGG + chr4.2:1425760-1425779 MS.gene62681:CDS 45.0%
ACCAGAGGCTTAAGAGCAAG+GGG + chr4.2:1425646-1425665 MS.gene62681:CDS 50.0%
ACTCGCAGCGCAATCAATTG+AGG - chr4.2:1425507-1425526 None:intergenic 50.0%
ATACTAGCCACATCCTGCGA+CGG - chr4.2:1425471-1425490 None:intergenic 50.0%
GAGGCCTGTAAAAGAGCTTG+TGG + chr4.2:1425572-1425591 MS.gene62681:CDS 50.0%
GGTCCTTCATTCCGTATTGG+TGG + chr4.2:1425759-1425778 MS.gene62681:CDS 50.0%
GTCGCAGGATGTGGCTAGTA+TGG + chr4.2:1425470-1425489 MS.gene62681:CDS 55.0%
TCCCCTTGCTCTTAAGCCTC+TGG - chr4.2:1425650-1425669 None:intergenic 55.0%
TGATCCGGAAGGGTTTGCGA+GGG + chr4.2:1425362-1425381 MS.gene62681:CDS 55.0%
TTGATCCGGAAGGGTTTGCG+AGG + chr4.2:1425361-1425380 MS.gene62681:CDS 55.0%
ACCACCCTCGCAAACCCTTC+CGG - chr4.2:1425369-1425388 None:intergenic 60.0%
GGCAATACCGTCGCAGGATG+TGG + chr4.2:1425461-1425480 MS.gene62681:CDS 60.0%
GGTGGAGAAGATGCCACAGG+TGG + chr4.2:1425386-1425405 MS.gene62681:CDS 60.0%
TGGTAGGGCAATACCGTCGC+AGG + chr4.2:1425455-1425474 MS.gene62681:CDS 60.0%
!! GGTGGTGGAGAAGATGCCAC+AGG + chr4.2:1425383-1425402 MS.gene62681:CDS 60.0%
GGAAGGGTTTGCGAGGGTGG+TGG + chr4.2:1425368-1425387 MS.gene62681:CDS 65.0%
TCCGGAAGGGTTTGCGAGGG+TGG + chr4.2:1425365-1425384 MS.gene62681:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 1424544 1425872 1424544 ID=MS.gene62681
chr4.2 mRNA 1424544 1425872 1424544 ID=MS.gene62681.t1;Parent=MS.gene62681
chr4.2 exon 1424544 1425872 1424544 ID=MS.gene62681.t1.exon1;Parent=MS.gene62681.t1
chr4.2 CDS 1424544 1425872 1424544 ID=cds.MS.gene62681.t1;Parent=MS.gene62681.t1
Gene Sequence

>MS.gene62681

ATGCCTACTGTTACATGGGGTGTAATTCAAGGTCGCAAAGAGAAACTCGTTTCGCGGGTCATCATCTTCGATTATCTCAAGGGTTTGGGAATTATTCCTGATGAATTGCAGGATTTGGAGCTTCCTTCAACCGTTGAAGTTATGAGGGAACGTGTTGAGTTTATTCAGAAATTAGGGTTAACGATTGATGATATAAATCAGTATCCTTTGATTTTGGGCTGTAGTGTTCGGAAAAACATGATTCCTGTTTTAGGGTATTTAGAAAAAATTGGGATTTCTAGGTCTAAACTTGGTGAATTTATTAAGAATTATCCACAAGTTTTGCATGCTAGTGTTATTGTTGAGCTTGCTCCTGTTATTAAGTTTCTTAGAGGACTTGATGTGGAGAAAGATGATATTGGATTTGTGTTGCAAAAATATCCTCAACTTTTAGGGTTTAAACTTGAAGGTACTATGAGTACTTCAGTAGCTTATCTTGTTAGTATTGGTGTTAATCCTAGGGATATTGGTCCAATGGTTACACAGTATCCTTATTTTTTAGGGATGAGAGTTGGTACTATGATAAAGCCTTTTGTTGATTACTTGGTTAATTTAGGGTTGCCTAAGAAGATTCTAGCTAGGATGTTGGAGAAAAGAGCTTATTTACTTGGCTATGATCTTGAAGAGACTGTGAAACCTAATGTTGATTGTTTGATTAGTTTTGGTTTGAGGAAGGAATGTTTGCCTTCGGTTATTGCTCAGTATCCGCAGATTATTGGGTTGCCTCTTAAGGCTAAATTGTCTTCCCAACAATATTTTTTCAGTTTGAAGCTTAAGATTGATCCGGAAGGGTTTGCGAGGGTGGTGGAGAAGATGCCACAGGTGGTTAGTCTTCATCAAAATGTGATTATGAAACCGGTTGAGTTTTTACTTGGTAGGGCAATACCGTCGCAGGATGTGGCTAGTATGGTGATCAAGTGTCCTCAATTGATTGCGCTGCGAGTTGAGCTCATGAAGAACAGTTATTACTTCTTCAAAAGTGAGATGGGGAGGCCTGTAAAAGAGCTTGTGGAGTTTCCAGAATACTTCACTTATAGTTTGGAATCAAGAATTAAACCTAGATACCAGAGGCTTAAGAGCAAGGGGATAAAATGTTCGTTGAATTGGATGCTGAATTGTAGTGACCAGAGATTTGAAGAGAGATTGCAGGGCAATTATATTGAAACCGAAAGTATAGGTCCTTCATTCCGTATTGGTGGGAAATTAGAGCTACCAGAAAACGACATAGTTTCAGATGAGGAAGAGGAGAGTGATGATGAAATGCTATACAGAAGAACGGTTTCTCTTTAG
Protein sequence

>MS.gene62681.t1

MPTVTWGVIQGRKEKLVSRVIIFDYLKGLGIIPDELQDLELPSTVEVMRERVEFIQKLGLTIDDINQYPLILGCSVRKNMIPVLGYLEKIGISRSKLGEFIKNYPQVLHASVIVELAPVIKFLRGLDVEKDDIGFVLQKYPQLLGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPFVDYLVNLGLPKKILARMLEKRAYLLGYDLEETVKPNVDCLISFGLRKECLPSVIAQYPQIIGLPLKAKLSSQQYFFSLKLKIDPEGFARVVEKMPQVVSLHQNVIMKPVEFLLGRAIPSQDVASMVIKCPQLIALRVELMKNSYYFFKSEMGRPVKELVEFPEYFTYSLESRIKPRYQRLKSKGIKCSLNWMLNCSDQRFEERLQGNYIETESIGPSFRIGGKLELPENDIVSDEEEESDDEMLYRRTVSL