AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63869

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63869.t1 MTR_5g094450 96.183 524 20 0 1 524 9 532 0.0 1055
MS.gene63869.t1 MTR_3g056110 66.174 541 159 8 1 524 9 542 0.0 711
MS.gene63869.t1 MTR_5g097480 58.379 543 195 8 5 524 10 544 0.0 620
MS.gene63869.t1 MTR_4g133660 55.085 472 206 4 53 523 76 542 1.29e-179 516
MS.gene63869.t1 MTR_2g082090 49.505 505 209 6 60 523 79 578 2.08e-167 486
MS.gene63869.t1 MTR_2g026250 55.928 388 166 3 138 524 213 596 1.91e-156 459
MS.gene63869.t1 MTR_3g089055 49.870 385 188 3 140 524 191 570 1.14e-136 407
MS.gene63869.t1 MTR_3g089055 49.870 385 188 3 140 524 186 565 3.03e-136 406
MS.gene63869.t1 MTR_1g029420 44.328 476 229 7 79 524 123 592 4.54e-136 407
MS.gene63869.t1 MTR_7g027190 33.333 459 270 7 84 524 233 673 8.73e-76 252
MS.gene63869.t1 MTR_8g442410 34.545 385 233 6 149 524 151 525 1.37e-68 230
MS.gene63869.t1 MTR_3g065980 35.407 418 226 11 128 524 146 540 3.88e-68 229
MS.gene63869.t1 MTR_4g097080 30.789 380 250 5 151 524 131 503 2.51e-61 209
MS.gene63869.t1 MTR_4g064160 31.579 380 247 6 149 523 312 683 7.34e-61 213
MS.gene63869.t1 MTR_2g097410 33.247 385 236 9 149 523 367 740 2.80e-60 212
MS.gene63869.t1 MTR_4g104020 31.443 388 240 7 150 524 146 520 7.28e-60 206
MS.gene63869.t1 MTR_7g062120 30.457 394 260 7 135 524 357 740 3.54e-59 209
MS.gene63869.t1 MTR_7g074650 35.772 369 218 8 157 524 442 792 6.04e-59 209
MS.gene63869.t1 MTR_3g022830 28.878 419 242 6 154 524 26 436 1.62e-58 201
MS.gene63869.t1 MTR_4g064150 32.637 383 243 8 149 523 357 732 1.98e-58 206
MS.gene63869.t1 MTR_4g064200 31.034 377 251 5 149 523 280 649 5.17e-57 201
MS.gene63869.t1 MTR_7g057230 34.211 380 228 9 151 523 292 656 7.41e-57 201
MS.gene63869.t1 MTR_4g102790 32.658 395 230 10 147 523 285 661 6.97e-56 199
MS.gene63869.t1 MTR_4g064120 31.481 378 250 7 149 521 250 623 1.64e-55 197
MS.gene63869.t1 MTR_2g097390 30.890 382 245 9 149 523 271 640 1.68e-55 197
MS.gene63869.t1 MTR_4g064180 31.662 379 248 7 149 521 250 623 1.99e-55 196
MS.gene63869.t1 MTR_2g097310 30.159 378 253 5 149 523 268 637 5.42e-55 196
MS.gene63869.t1 MTR_2g097350 31.070 383 243 11 149 523 270 639 1.98e-54 194
MS.gene63869.t1 MTR_6g047750 33.760 391 221 11 151 524 293 662 2.58e-53 191
MS.gene63869.t1 MTR_5g015490 30.526 380 253 6 153 524 114 490 1.26e-52 186
MS.gene63869.t1 MTR_2g097467 30.053 376 255 4 149 523 285 653 3.44e-52 188
MS.gene63869.t1 MTR_2g097473 31.034 377 250 6 149 523 285 653 2.47e-51 186
MS.gene63869.t1 MTR_2g097463 30.688 378 250 6 149 523 286 654 7.39e-50 182
MS.gene63869.t1 MTR_4g077760 34.078 358 215 9 173 524 213 555 6.28e-49 177
MS.gene63869.t1 MTR_1g096030 29.902 408 228 16 150 524 77 459 7.18e-47 170
MS.gene63869.t1 MTR_4g076020 32.432 370 220 9 157 524 88 429 3.60e-46 167
MS.gene63869.t1 MTR_2g089100 25.799 438 293 7 112 523 11 442 1.67e-45 166
MS.gene63869.t1 MTR_2g097380 27.248 367 231 6 158 523 229 560 1.07e-42 160
MS.gene63869.t1 MTR_1g069725 29.684 411 261 13 118 523 75 462 2.63e-41 155
MS.gene63869.t1 MTR_2g099110 29.873 395 239 10 152 524 73 451 1.04e-40 152
MS.gene63869.t1 MTR_3g072710 28.718 390 255 9 150 523 117 499 3.81e-39 149
MS.gene63869.t1 MTR_2g034250 27.506 389 258 8 150 524 207 585 3.63e-37 145
MS.gene63869.t1 MTR_5g015950 24.429 438 265 9 139 523 21 445 9.90e-36 139
MS.gene63869.t1 MTR_3g053270 24.429 438 265 9 139 523 21 445 9.90e-36 139
MS.gene63869.t1 MTR_5g058860 29.082 392 249 11 150 523 116 496 3.38e-35 138
MS.gene63869.t1 MTR_4g095500 25.362 414 267 8 150 523 56 467 7.02e-35 137
MS.gene63869.t1 MTR_2g034260 25.635 394 258 10 150 524 207 584 8.33e-32 129
MS.gene63869.t1 MTR_4g026485 26.790 377 248 10 154 524 270 624 1.38e-31 129
MS.gene63869.t1 MTR_2g034280 25.195 385 269 10 150 524 200 575 5.03e-31 127
MS.gene63869.t1 MTR_5g009080 28.972 214 144 3 150 363 53 258 3.69e-29 120
MS.gene63869.t1 MTR_5g009080 36.879 141 88 1 385 524 338 478 2.24e-24 106
MS.gene63869.t1 MTR_4g076140 31.111 225 146 3 138 361 32 248 3.91e-29 120
MS.gene63869.t1 MTR_4g076140 38.298 141 86 1 385 524 329 469 1.62e-26 112
MS.gene63869.t1 MTR_7g109580 25.840 387 259 11 149 524 185 554 2.10e-28 119
MS.gene63869.t1 MTR_0092s0100 24.665 373 262 7 154 524 373 728 7.77e-27 115
MS.gene63869.t1 MTR_0092s0100 24.665 373 262 7 154 524 376 731 8.24e-27 115
MS.gene63869.t1 MTR_8g093070 25.968 439 281 17 102 523 84 495 1.18e-25 110
MS.gene63869.t1 MTR_8g020840 25.436 401 251 15 151 524 169 548 6.58e-23 102
MS.gene63869.t1 MTR_1g086970 24.173 393 265 14 149 524 102 478 4.19e-20 93.6
MS.gene63869.t1 MTR_3g022580 34.545 110 72 0 415 524 74 183 4.30e-17 79.7
MS.gene63869.t1 MTR_3g022005 38.710 93 57 0 432 524 33 125 3.62e-16 75.5
MS.gene63869.t1 MTR_8g077940 23.773 387 261 13 149 524 178 541 3.91e-15 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63869.t1 AT1G50600 56.309 531 204 8 5 524 84 597 0.0 566
MS.gene63869.t1 AT5G48150 62.727 440 154 4 86 524 60 490 0.0 565
MS.gene63869.t1 AT5G48150 62.727 440 154 4 86 524 60 490 0.0 565
MS.gene63869.t1 AT5G48150 62.727 440 154 4 86 524 60 490 0.0 565
MS.gene63869.t1 AT5G48150 62.727 440 154 4 86 524 60 490 0.0 565
MS.gene63869.t1 AT1G50600 56.309 531 204 8 5 524 88 601 0.0 565
MS.gene63869.t1 AT1G50600 56.309 531 204 8 5 524 13 526 0.0 564
MS.gene63869.t1 AT2G04890 61.719 384 136 3 141 524 41 413 1.31e-175 501
MS.gene63869.t1 AT4G17230 48.256 516 232 7 29 524 25 525 2.76e-158 461
MS.gene63869.t1 AT4G17230 48.256 516 232 7 29 524 25 525 2.76e-158 461
MS.gene63869.t1 AT1G21450 46.429 448 222 5 91 524 150 593 2.23e-138 412
MS.gene63869.t1 AT1G66350 35.884 379 215 10 150 524 152 506 5.58e-70 233
MS.gene63869.t1 AT1G14920 35.789 380 220 11 150 524 169 529 3.77e-67 226
MS.gene63869.t1 AT3G03450 35.340 382 224 9 150 524 180 545 4.26e-67 226
MS.gene63869.t1 AT2G01570 36.745 381 215 12 150 524 221 581 2.27e-66 225
MS.gene63869.t1 AT1G07530 33.862 378 244 4 148 523 391 764 5.16e-66 228
MS.gene63869.t1 AT5G17490 34.465 383 220 9 150 524 157 516 2.21e-65 221
MS.gene63869.t1 AT2G29060 34.293 382 237 6 149 523 280 654 5.83e-61 212
MS.gene63869.t1 AT2G29060 34.293 382 237 6 149 523 317 691 1.32e-60 212
MS.gene63869.t1 AT5G52510 33.842 393 223 9 151 524 266 640 2.55e-60 210
MS.gene63869.t1 AT3G54220 36.043 369 218 8 157 523 297 649 1.91e-59 208
MS.gene63869.t1 AT3G46600 30.446 381 249 4 150 524 1 371 2.04e-56 193
MS.gene63869.t1 AT5G41920 35.417 384 216 13 150 524 40 400 2.95e-56 194
MS.gene63869.t1 AT3G46600 30.628 382 249 4 149 524 176 547 7.93e-56 196
MS.gene63869.t1 AT3G46600 30.628 382 249 4 149 524 208 579 9.49e-56 196
MS.gene63869.t1 AT5G59450 29.737 380 259 5 149 524 223 598 8.87e-55 194
MS.gene63869.t1 AT1G07520 28.442 443 292 10 100 523 92 528 2.37e-54 192
MS.gene63869.t1 AT1G07520 28.442 443 292 10 100 523 92 528 2.37e-54 192
MS.gene63869.t1 AT1G07520 28.442 443 292 10 100 523 256 692 1.20e-53 193
MS.gene63869.t1 AT1G07520 28.442 443 292 10 100 523 257 693 1.27e-53 192
MS.gene63869.t1 AT2G37650 29.973 377 257 5 149 524 331 701 1.10e-52 190
MS.gene63869.t1 AT2G37650 29.973 377 257 5 149 524 343 713 1.20e-52 190
MS.gene63869.t1 AT2G29065 30.366 382 257 5 149 523 248 627 1.29e-52 189
MS.gene63869.t1 AT5G66770 33.333 381 235 8 150 524 217 584 3.80e-51 184
MS.gene63869.t1 AT1G55580 30.361 415 239 9 150 524 41 445 4.32e-50 178
MS.gene63869.t1 AT1G63100 30.769 390 246 6 149 523 248 628 3.08e-49 179
MS.gene63869.t1 AT1G63100 30.769 390 246 6 149 523 273 653 4.56e-49 179
MS.gene63869.t1 AT3G50650 31.783 387 237 8 149 524 172 542 1.19e-48 176
MS.gene63869.t1 AT4G37650 28.934 394 248 11 153 524 146 529 1.90e-48 176
MS.gene63869.t1 AT3G49950 23.896 385 278 5 150 523 27 407 3.91e-35 136
MS.gene63869.t1 AT4G08250 28.990 307 201 6 225 523 179 476 2.63e-34 135
MS.gene63869.t1 AT1G50420 31.604 212 139 1 150 361 54 259 1.67e-30 124
MS.gene63869.t1 AT1G50420 38.028 142 85 2 385 524 339 479 2.10e-26 112
MS.gene63869.t1 AT3G60630 27.401 354 220 10 186 524 291 622 2.76e-24 107
MS.gene63869.t1 AT2G45160 26.579 380 222 15 169 524 293 639 1.38e-23 105
MS.gene63869.t1 AT4G00150 24.557 395 233 15 150 524 205 554 2.60e-21 97.8
MS.gene63869.t1 AT4G36710 25.707 389 252 13 149 524 121 485 4.71e-21 96.7
MS.gene63869.t1 AT3G13840 24.552 391 252 12 151 524 146 510 9.83e-21 95.9
MS.gene63869.t1 AT5G67411 31.683 202 122 6 272 461 1 198 2.94e-16 77.8

Find 95 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGAAAATGGTTTCGGTTTC+AGG 0.082618 5.1:-75724206 MS.gene63869:CDS
ATTTGTCCTTACCTCAAATT+TGG 0.139278 5.1:-75724029 MS.gene63869:CDS
TCTTTACTAGTAGCAGTTAT+AGG 0.224168 5.1:+75724083 None:intergenic
GCATTTGAGTTTCATGCTAT+AGG 0.322420 5.1:-75723759 MS.gene63869:CDS
CATTTGAGTTTCATGCTATA+GGG 0.329203 5.1:-75723758 MS.gene63869:CDS
CAATTAATATAAGAGCTTAA+TGG 0.338228 5.1:+75723300 None:intergenic
AGCAATATCAGAAAATGATA+TGG 0.350309 5.1:-75724258 MS.gene63869:CDS
TATAGGCTCTTTGCATTTCA+AGG 0.357284 5.1:+75724100 None:intergenic
ATATAAGAGCTTAATGGATA+TGG 0.358531 5.1:+75723306 None:intergenic
ACAAGAATCTGATCCAGTTT+TGG 0.361902 5.1:-75724360 MS.gene63869:CDS
TCAACCTACCCTTCATTTCA+AGG 0.362889 5.1:-75724620 MS.gene63869:CDS
GTTGCAAGAATATCTTCTTC+AGG 0.365444 5.1:-75724140 MS.gene63869:CDS
AATGTCAGAGTTGTCGAAAA+TGG 0.368522 5.1:-75724219 MS.gene63869:CDS
TTCCTGCAAGTGCTTGGATT+AGG 0.369074 5.1:+75723907 None:intergenic
CTGATCTTCGGCGGTCCTCC+AGG 0.371989 5.1:+75723882 None:intergenic
AAAGATGGTGCTCTTTATCT+TGG 0.376459 5.1:-75723225 MS.gene63869:CDS
ACATGTATGCTCCTAACCTT+TGG 0.380505 5.1:+75724174 None:intergenic
CTGACATGTATCCAAATTTG+AGG 0.406987 5.1:+75724018 None:intergenic
TGTTCTAAACCTTGAAATGA+AGG 0.411155 5.1:+75724611 None:intergenic
AGCCTAATCCAAGCACTTGC+AGG 0.415726 5.1:-75723909 MS.gene63869:CDS
CTTCACCGCGACGAAGTTCA+AGG 0.420273 5.1:+75723706 None:intergenic
GACTTGGTATTGTTGGAGAA+AGG 0.422984 5.1:-75723812 MS.gene63869:CDS
CAAGCACTTGCAGGAAAACC+TGG 0.425854 5.1:-75723900 MS.gene63869:CDS
TATGCTCCTAACCTTTGGAT+TGG 0.428646 5.1:+75724179 None:intergenic
TCGACGAAACGTGCAAAGAA+TGG 0.431715 5.1:+75723528 None:intergenic
GGCAAAATGTTTGTCTCCTA+AGG 0.436222 5.1:-75723592 MS.gene63869:CDS
TCTTGTGCCACAAGTCATCT+TGG 0.436674 5.1:+75724462 None:intergenic
GACTATTGTTAGAAGATTCA+AGG 0.438602 5.1:+75724585 None:intergenic
GGAGGAGGACTTGGTATTGT+TGG 0.447375 5.1:-75723819 MS.gene63869:CDS
ATGGTGCTCTTTATCTTGGT+TGG 0.450648 5.1:-75723221 MS.gene63869:CDS
CTCTTATTACCTCTAGTGCT+TGG 0.454394 5.1:-75723188 MS.gene63869:CDS
CTTGTAGAGTATAATGTCCT+TGG 0.456322 5.1:+75723250 None:intergenic
AAACCGGTTATTCTGATCTT+CGG 0.458599 5.1:+75723870 None:intergenic
TATGCTCGGGGAGGAGGACT+TGG 0.465345 5.1:-75723828 MS.gene63869:CDS
GGAAATCCAATCCAAAGGTT+AGG 0.465445 5.1:-75724185 MS.gene63869:CDS
AGAGTTGTCGAAAATGGTTT+CGG 0.470075 5.1:-75724213 MS.gene63869:CDS
AGATCACGTTTCACAATGGC+TGG 0.474283 5.1:-75723336 MS.gene63869:CDS
TCAGCTTATGCTCGGGGAGG+AGG 0.477295 5.1:-75723834 MS.gene63869:CDS
TCGCGATCGATTAGTGAGAT+TGG 0.480871 5.1:-75723613 MS.gene63869:CDS
ATCCTTTCTCTGCGCTCCCT+TGG 0.480887 5.1:+75723456 None:intergenic
CTCAACTTCAGCTTATGCTC+GGG 0.481355 5.1:-75723841 MS.gene63869:CDS
CCGCCGAAGATCAGAATAAC+CGG 0.481757 5.1:-75723873 MS.gene63869:CDS
TATGAGTCTAGATCTTGTAT+AGG 0.488577 5.1:+75724701 None:intergenic
CAAGCACTAGAGGTAATAAG+AGG 0.490940 5.1:+75723189 None:intergenic
CAATTGATGTTGCTTTGCCA+AGG 0.496515 5.1:-75723473 MS.gene63869:CDS
TCAACAAGAGTCACTACCTT+AGG 0.498490 5.1:+75723576 None:intergenic
GAAATGAAGGGTAGGTTGAA+TGG 0.500307 5.1:+75724624 None:intergenic
TTTGGATACATGTCAGCAAA+TGG 0.500743 5.1:-75724011 MS.gene63869:CDS
TGAAGTTGAGTCATCAAAAC+CGG 0.501919 5.1:+75723854 None:intergenic
TAATGTGGAACAGCATTGCT+TGG 0.522983 5.1:-75723433 MS.gene63869:CDS
TGAATCTTCTAACAATAGTC+CGG 0.523182 5.1:-75724582 MS.gene63869:CDS
AACTACTCATCTTCTGATAA+CGG 0.524904 5.1:-75724650 MS.gene63869:CDS
TGCCAAGGGAGCGCAGAGAA+AGG 0.528262 5.1:-75723458 MS.gene63869:CDS
GAGCTTGAAAATGCTATGCT+AGG 0.532196 5.1:-75724434 MS.gene63869:CDS
GTACATCTTCATCCGGTACA+TGG 0.543566 5.1:+75723652 None:intergenic
ACTCAACTTCAGCTTATGCT+CGG 0.546988 5.1:-75723842 MS.gene63869:CDS
CCACCATGTACATCTTCATC+CGG 0.547887 5.1:+75723645 None:intergenic
ATGTATCCAAATTTGAGGTA+AGG 0.552106 5.1:+75724023 None:intergenic
TTTGACTGTAGCTTCGAGAC+CGG 0.552976 5.1:+75724563 None:intergenic
ATTTGAGTTTCATGCTATAG+GGG 0.555615 5.1:-75723757 MS.gene63869:CDS
ACTATTGTTAGAAGATTCAA+GGG 0.562160 5.1:+75724586 None:intergenic
GTCACGAGGTTCTGAAGAAG+TGG 0.562877 5.1:-75723359 MS.gene63869:CDS
TCTTCAAGTCTCACCTCAGA+AGG 0.563852 5.1:+75723729 None:intergenic
ATGATATGGAAACAGCTGAA+TGG 0.565722 5.1:-75724244 MS.gene63869:CDS
CGGTACATGGTGGAGCATCA+TGG 0.567712 5.1:+75723665 None:intergenic
GTTCTAAACCTTGAAATGAA+GGG 0.567885 5.1:+75724612 None:intergenic
CATGATGCTCCACCATGTAC+CGG 0.570910 5.1:-75723664 MS.gene63869:CDS
AATTGATGTTGCTTTGCCAA+GGG 0.571262 5.1:-75723472 MS.gene63869:CDS
TAATTGAGATGATATCTAGA+GGG 0.584315 5.1:-75724313 MS.gene63869:CDS
TGCAAAGAATGGAAGCTCGT+TGG 0.585649 5.1:+75723539 None:intergenic
GTCAATTTAGTAGCGTGTGA+AGG 0.591391 5.1:-75723399 MS.gene63869:CDS
AGATTAATCAGGGAATTCAG+TGG 0.595212 5.1:-75723935 MS.gene63869:CDS
TAAACCTTGAAATGAAGGGT+AGG 0.600145 5.1:+75724616 None:intergenic
GCATTGCTTGGCTCGCGAAG+TGG 0.604747 5.1:-75723421 MS.gene63869:CDS
TATACTCTACAAGAGAAAGA+TGG 0.606593 5.1:-75723240 MS.gene63869:CDS
ATAATTGAGATGATATCTAG+AGG 0.620492 5.1:-75724314 MS.gene63869:CDS
TTTCAGGAAATCCAATCCAA+AGG 0.621360 5.1:-75724190 MS.gene63869:CDS
CACATAACCAAGATGACTTG+TGG 0.627441 5.1:-75724469 MS.gene63869:CDS
AATTGAGATGATATCTAGAG+GGG 0.628425 5.1:-75724312 MS.gene63869:CDS
TCGTCGCGGTGAAGCTATTG+CGG 0.629040 5.1:-75723697 MS.gene63869:CDS
GCGCAGAGAAAGGATTAATG+TGG 0.629496 5.1:-75723448 MS.gene63869:CDS
AAGGTTAGGAGCATACATGT+TGG 0.640121 5.1:-75724171 MS.gene63869:CDS
GTATCATTTCCAAGCACTAG+AGG 0.640399 5.1:+75723179 None:intergenic
TATAGGGGCTAGTCCTTCTG+AGG 0.643175 5.1:-75723742 MS.gene63869:CDS
CCGGTTATTCTGATCTTCGG+CGG 0.646295 5.1:+75723873 None:intergenic
TCAACTTCAGCTTATGCTCG+GGG 0.671597 5.1:-75723840 MS.gene63869:CDS
CATCTTCATCCGGTACATGG+TGG 0.673917 5.1:+75723655 None:intergenic
GTGGAGATCACGTTTCACAA+TGG 0.676222 5.1:-75723340 MS.gene63869:CDS
AGAGAGAGTTGAACGTCACG+AGG 0.678622 5.1:-75723373 MS.gene63869:CDS
ACTTCAGCTTATGCTCGGGG+AGG 0.692496 5.1:-75723837 MS.gene63869:CDS
AATCTTCTAGAAAATTACCA+AGG 0.696750 5.1:-75723267 MS.gene63869:CDS
GTACCGGATGAAGATGTACA+TGG 0.718087 5.1:-75723648 MS.gene63869:CDS
GAAGACCTTGAACTTCGTCG+CGG 0.723803 5.1:-75723711 MS.gene63869:CDS
GCACTTGCAGGAAAACCTGG+AGG 0.760967 5.1:-75723897 MS.gene63869:CDS
CCGGATGAAGATGTACATGG+TGG 0.827271 5.1:-75723645 MS.gene63869:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTAATATAAGAGCTTAA+TGG + chr5.1:75724619-75724638 None:intergenic 20.0%
!!! AATTGATTTTCAGATTAATC+AGG - chr5.1:75723970-75723989 MS.gene63869:CDS 20.0%
!!! ATTGATTTTCAGATTAATCA+GGG - chr5.1:75723971-75723990 MS.gene63869:CDS 20.0%
! ACTATTGTTAGAAGATTCAA+GGG + chr5.1:75723333-75723352 None:intergenic 25.0%
! AGCAATATCAGAAAATGATA+TGG - chr5.1:75723658-75723677 MS.gene63869:CDS 25.0%
! ATAATTGAGATGATATCTAG+AGG - chr5.1:75723602-75723621 MS.gene63869:CDS 25.0%
! TAATTGAGATGATATCTAGA+GGG - chr5.1:75723603-75723622 MS.gene63869:CDS 25.0%
!! AATCTTCTAGAAAATTACCA+AGG - chr5.1:75724649-75724668 MS.gene63869:CDS 25.0%
!! ATATAAGAGCTTAATGGATA+TGG + chr5.1:75724613-75724632 None:intergenic 25.0%
!!! AACTTCACTTTCATTTTTCA+AGG + chr5.1:75723945-75723964 None:intergenic 25.0%
AACTACTCATCTTCTGATAA+CGG - chr5.1:75723266-75723285 MS.gene63869:CDS 30.0%
AATTGAGATGATATCTAGAG+GGG - chr5.1:75723604-75723623 MS.gene63869:CDS 30.0%
ATTTGTCCTTACCTCAAATT+TGG - chr5.1:75723887-75723906 MS.gene63869:CDS 30.0%
GACTATTGTTAGAAGATTCA+AGG + chr5.1:75723334-75723353 None:intergenic 30.0%
TATACTCTACAAGAGAAAGA+TGG - chr5.1:75724676-75724695 MS.gene63869:CDS 30.0%
TATGAGTCTAGATCTTGTAT+AGG + chr5.1:75723218-75723237 None:intergenic 30.0%
TCTTTACTAGTAGCAGTTAT+AGG + chr5.1:75723836-75723855 None:intergenic 30.0%
TGAATCTTCTAACAATAGTC+CGG - chr5.1:75723334-75723353 MS.gene63869:CDS 30.0%
! ATGTATCCAAATTTGAGGTA+AGG + chr5.1:75723896-75723915 None:intergenic 30.0%
! ATTTGAGTTTCATGCTATAG+GGG - chr5.1:75724159-75724178 MS.gene63869:CDS 30.0%
! CATTTGAGTTTCATGCTATA+GGG - chr5.1:75724158-75724177 MS.gene63869:CDS 30.0%
! GTTCTAAACCTTGAAATGAA+GGG + chr5.1:75723307-75723326 None:intergenic 30.0%
! TGTTCTAAACCTTGAAATGA+AGG + chr5.1:75723308-75723327 None:intergenic 30.0%
!! TTTCGAGATTCTCATTTTTC+AGG + chr5.1:75723247-75723266 None:intergenic 30.0%
AAACCGGTTATTCTGATCTT+CGG + chr5.1:75724049-75724068 None:intergenic 35.0%
AAAGATGGTGCTCTTTATCT+TGG - chr5.1:75724691-75724710 MS.gene63869:CDS 35.0%
AATGTCAGAGTTGTCGAAAA+TGG - chr5.1:75723697-75723716 MS.gene63869:CDS 35.0%
AGAGTTGTCGAAAATGGTTT+CGG - chr5.1:75723703-75723722 MS.gene63869:CDS 35.0%
AGATTAATCAGGGAATTCAG+TGG - chr5.1:75723981-75724000 MS.gene63869:CDS 35.0%
ATGATATGGAAACAGCTGAA+TGG - chr5.1:75723672-75723691 MS.gene63869:CDS 35.0%
CTGACATGTATCCAAATTTG+AGG + chr5.1:75723901-75723920 None:intergenic 35.0%
CTTGTAGAGTATAATGTCCT+TGG + chr5.1:75724669-75724688 None:intergenic 35.0%
GTTGCAAGAATATCTTCTTC+AGG - chr5.1:75723776-75723795 MS.gene63869:CDS 35.0%
TAAACCTTGAAATGAAGGGT+AGG + chr5.1:75723303-75723322 None:intergenic 35.0%
TATAGGCTCTTTGCATTTCA+AGG + chr5.1:75723819-75723838 None:intergenic 35.0%
TGAAGTTGAGTCATCAAAAC+CGG + chr5.1:75724065-75724084 None:intergenic 35.0%
TTTCAGGAAATCCAATCCAA+AGG - chr5.1:75723726-75723745 MS.gene63869:CDS 35.0%
TTTGGATACATGTCAGCAAA+TGG - chr5.1:75723905-75723924 MS.gene63869:CDS 35.0%
! ACAAGAATCTGATCCAGTTT+TGG - chr5.1:75723556-75723575 MS.gene63869:CDS 35.0%
! GCATTTGAGTTTCATGCTAT+AGG - chr5.1:75724157-75724176 MS.gene63869:CDS 35.0%
!! AATTGATGTTGCTTTGCCAA+GGG - chr5.1:75724444-75724463 MS.gene63869:CDS 35.0%
ACATGTATGCTCCTAACCTT+TGG + chr5.1:75723745-75723764 None:intergenic 40.0%
ACTCAACTTCAGCTTATGCT+CGG - chr5.1:75724074-75724093 MS.gene63869:CDS 40.0%
ATGGTGCTCTTTATCTTGGT+TGG - chr5.1:75724695-75724714 MS.gene63869:CDS 40.0%
CAAGCACTAGAGGTAATAAG+AGG + chr5.1:75724730-75724749 None:intergenic 40.0%
CACATAACCAAGATGACTTG+TGG - chr5.1:75723447-75723466 MS.gene63869:CDS 40.0%
CTCTTATTACCTCTAGTGCT+TGG - chr5.1:75724728-75724747 MS.gene63869:CDS 40.0%
GAGCTTGAAAATGCTATGCT+AGG - chr5.1:75723482-75723501 MS.gene63869:CDS 40.0%
GGAAATCCAATCCAAAGGTT+AGG - chr5.1:75723731-75723750 MS.gene63869:CDS 40.0%
GGCAAAATGTTTGTCTCCTA+AGG - chr5.1:75724324-75724343 MS.gene63869:CDS 40.0%
GTCAATTTAGTAGCGTGTGA+AGG - chr5.1:75724517-75724536 MS.gene63869:CDS 40.0%
TAATGTGGAACAGCATTGCT+TGG - chr5.1:75724483-75724502 MS.gene63869:CDS 40.0%
TATGCTCCTAACCTTTGGAT+TGG + chr5.1:75723740-75723759 None:intergenic 40.0%
TCAACAAGAGTCACTACCTT+AGG + chr5.1:75724343-75724362 None:intergenic 40.0%
TCAACCTACCCTTCATTTCA+AGG - chr5.1:75723296-75723315 MS.gene63869:CDS 40.0%
TCGAAAATGGTTTCGGTTTC+AGG - chr5.1:75723710-75723729 MS.gene63869:CDS 40.0%
! AAGGTTAGGAGCATACATGT+TGG - chr5.1:75723745-75723764 MS.gene63869:CDS 40.0%
! GAAATGAAGGGTAGGTTGAA+TGG + chr5.1:75723295-75723314 None:intergenic 40.0%
! GACTTGGTATTGTTGGAGAA+AGG - chr5.1:75724104-75724123 MS.gene63869:CDS 40.0%
!! ATCTGATCCAGTTTTGGTAG+AGG - chr5.1:75723562-75723581 MS.gene63869:CDS 40.0%
!! CAATTGATGTTGCTTTGCCA+AGG - chr5.1:75724443-75724462 MS.gene63869:CDS 40.0%
AGATCACGTTTCACAATGGC+TGG - chr5.1:75724580-75724599 MS.gene63869:CDS 45.0%
CCACCATGTACATCTTCATC+CGG + chr5.1:75724274-75724293 None:intergenic 45.0%
CTCAACTTCAGCTTATGCTC+GGG - chr5.1:75724075-75724094 MS.gene63869:CDS 45.0%
GCGCAGAGAAAGGATTAATG+TGG - chr5.1:75724468-75724487 MS.gene63869:CDS 45.0%
GTACATCTTCATCCGGTACA+TGG + chr5.1:75724267-75724286 None:intergenic 45.0%
GTACCGGATGAAGATGTACA+TGG - chr5.1:75724268-75724287 MS.gene63869:CDS 45.0%
GTGGAGATCACGTTTCACAA+TGG - chr5.1:75724576-75724595 MS.gene63869:CDS 45.0%
TCAACTTCAGCTTATGCTCG+GGG - chr5.1:75724076-75724095 MS.gene63869:CDS 45.0%
TCGACGAAACGTGCAAAGAA+TGG + chr5.1:75724391-75724410 None:intergenic 45.0%
TCGCGATCGATTAGTGAGAT+TGG - chr5.1:75724303-75724322 MS.gene63869:CDS 45.0%
TCTTCAAGTCTCACCTCAGA+AGG + chr5.1:75724190-75724209 None:intergenic 45.0%
TCTTGTGCCACAAGTCATCT+TGG + chr5.1:75723457-75723476 None:intergenic 45.0%
TGCAAAGAATGGAAGCTCGT+TGG + chr5.1:75724380-75724399 None:intergenic 45.0%
TTCCTGCAAGTGCTTGGATT+AGG + chr5.1:75724012-75724031 None:intergenic 45.0%
TTTGACTGTAGCTTCGAGAC+CGG + chr5.1:75723356-75723375 None:intergenic 45.0%
! TTTTCCGCCTCTACCAAAAC+TGG + chr5.1:75723572-75723591 None:intergenic 45.0%
! TTTTGGTAGAGGCGGAAAAG+TGG - chr5.1:75723573-75723592 MS.gene63869:CDS 45.0%
!! TGATCCAGTTTTGGTAGAGG+CGG - chr5.1:75723565-75723584 MS.gene63869:CDS 45.0%
AGAGAGAGTTGAACGTCACG+AGG - chr5.1:75724543-75724562 MS.gene63869:CDS 50.0%
AGCCTAATCCAAGCACTTGC+AGG - chr5.1:75724007-75724026 MS.gene63869:CDS 50.0%
CAAGCACTTGCAGGAAAACC+TGG - chr5.1:75724016-75724035 MS.gene63869:CDS 50.0%
CATCTTCATCCGGTACATGG+TGG + chr5.1:75724264-75724283 None:intergenic 50.0%
CATGATGCTCCACCATGTAC+CGG - chr5.1:75724252-75724271 MS.gene63869:CDS 50.0%
CCGCCGAAGATCAGAATAAC+CGG - chr5.1:75724043-75724062 MS.gene63869:CDS 50.0%
CCGGATGAAGATGTACATGG+TGG - chr5.1:75724271-75724290 MS.gene63869:CDS 50.0%
CCGGTTATTCTGATCTTCGG+CGG + chr5.1:75724046-75724065 None:intergenic 50.0%
GAAGACCTTGAACTTCGTCG+CGG - chr5.1:75724205-75724224 MS.gene63869:CDS 50.0%
! CAGGTTTTCCTGCAAGTGCT+TGG + chr5.1:75724018-75724037 None:intergenic 50.0%
! GGAGGAGGACTTGGTATTGT+TGG - chr5.1:75724097-75724116 MS.gene63869:CDS 50.0%
!! GTCACGAGGTTCTGAAGAAG+TGG - chr5.1:75724557-75724576 MS.gene63869:CDS 50.0%
!! TATAGGGGCTAGTCCTTCTG+AGG - chr5.1:75724174-75724193 MS.gene63869:CDS 50.0%
ACTTCAGCTTATGCTCGGGG+AGG - chr5.1:75724079-75724098 MS.gene63869:CDS 55.0%
ATCCTTTCTCTGCGCTCCCT+TGG + chr5.1:75724463-75724482 None:intergenic 55.0%
CTTCACCGCGACGAAGTTCA+AGG + chr5.1:75724213-75724232 None:intergenic 55.0%
GCACTTGCAGGAAAACCTGG+AGG - chr5.1:75724019-75724038 MS.gene63869:CDS 55.0%
TCGTCGCGGTGAAGCTATTG+CGG - chr5.1:75724219-75724238 MS.gene63869:CDS 55.0%
! CGGTACATGGTGGAGCATCA+TGG + chr5.1:75724254-75724273 None:intergenic 55.0%
TATGCTCGGGGAGGAGGACT+TGG - chr5.1:75724088-75724107 MS.gene63869:CDS 60.0%
TCAGCTTATGCTCGGGGAGG+AGG - chr5.1:75724082-75724101 MS.gene63869:CDS 60.0%
! GCATTGCTTGGCTCGCGAAG+TGG - chr5.1:75724495-75724514 MS.gene63869:CDS 60.0%
! TGCCAAGGGAGCGCAGAGAA+AGG - chr5.1:75724458-75724477 MS.gene63869:CDS 60.0%
CTGATCTTCGGCGGTCCTCC+AGG + chr5.1:75724037-75724056 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 75723182 75724756 75723182 ID=MS.gene63869
chr5.1 mRNA 75723182 75724756 75723182 ID=MS.gene63869.t1;Parent=MS.gene63869
chr5.1 exon 75723182 75724756 75723182 ID=MS.gene63869.t1.exon1;Parent=MS.gene63869.t1
chr5.1 CDS 75723182 75724756 75723182 ID=cds.MS.gene63869.t1;Parent=MS.gene63869.t1
Gene Sequence

>MS.gene63869

ATGTCATATGATTCTAGCATGTTCAGCATTGAGCCTATACAAGATCTAGACTCATATTGCTTCCTGAAAAATGAGAATCTCGAAAACTACTCATCTTCTGATAACGGTAGCCATTCAACCTACCCTTCATTTCAAGGTTTAGAACAAACCCTTGAATCTTCTAACAATAGTCCGGTCTCGAAGCTACAGTCAAAATCTTACACGTTCACGTCACGAAACTCTCTTGAAATCATCAATGATTCTTTAGAGAACGAATCTTGCTTGACACATAACCAAGATGACTTGTGGCACAAGATAAGAGAGCTTGAAAATGCTATGCTAGGACATGATGCTGCAGATATGCTAGACATATATAATGATACTGTAATAATTCAACAAGAATCTGATCCAGTTTTGGTAGAGGCGGAAAAGTGGAACAAAATAATTGAGATGATATCTAGAGGGGATTTGAAAGAAATTCTTTTTACATGTGCAAAAGCAATATCAGAAAATGATATGGAAACAGCTGAATGGTTAATGTCAGAGTTGTCGAAAATGGTTTCGGTTTCAGGAAATCCAATCCAAAGGTTAGGAGCATACATGTTGGAAGCTCTTGTTGCAAGAATATCTTCTTCAGGAAGCATAATATACAAATCCTTGAAATGCAAAGAGCCTATAACTGCTACTAGTAAAGAACTACTCTCACACATGCATGTGCTTTATGAAATTTGTCCTTACCTCAAATTTGGATACATGTCAGCAAATGGTGTAATTGCTGAAGCCTTGAAAAATGAAAGTGAAGTTCATATAATTGATTTTCAGATTAATCAGGGAATTCAGTGGATGAGCCTAATCCAAGCACTTGCAGGAAAACCTGGAGGACCGCCGAAGATCAGAATAACCGGTTTTGATGACTCAACTTCAGCTTATGCTCGGGGAGGAGGACTTGGTATTGTTGGAGAAAGGTTATCAAAACTTGCACAGTCATATAATGTAGCATTTGAGTTTCATGCTATAGGGGCTAGTCCTTCTGAGGTGAGACTTGAAGACCTTGAACTTCGTCGCGGTGAAGCTATTGCGGTGAATTTTGCCATGATGCTCCACCATGTACCGGATGAAGATGTACATGGTGGTAAGAATCATCGCGATCGATTAGTGAGATTGGCAAAATGTTTGTCTCCTAAGGTAGTGACTCTTGTTGAGCAAGAATCAAATACCAACGAGCTTCCATTCTTTGCACGTTTCGTCGAGACAATGAACTACTACTTCGCAGTTTTTGAATCAATTGATGTTGCTTTGCCAAGGGAGCGCAGAGAAAGGATTAATGTGGAACAGCATTGCTTGGCTCGCGAAGTGGTCAATTTAGTAGCGTGTGAAGGCGCAGAGAGAGTTGAACGTCACGAGGTTCTGAAGAAGTGGAGATCACGTTTCACAATGGCTGGATTCACACCATATCCATTAAGCTCTTATATTAATTGTTCAATACAAAATCTTCTAGAAAATTACCAAGGACATTATACTCTACAAGAGAAAGATGGTGCTCTTTATCTTGGTTGGATGAATCAACCTCTTATTACCTCTAGTGCTTGGAAATGA
Protein sequence

>MS.gene63869.t1

MSYDSSMFSIEPIQDLDSYCFLKNENLENYSSSDNGSHSTYPSFQGLEQTLESSNNSPVSKLQSKSYTFTSRNSLEIINDSLENESCLTHNQDDLWHKIRELENAMLGHDAADMLDIYNDTVIIQQESDPVLVEAEKWNKIIEMISRGDLKEILFTCAKAISENDMETAEWLMSELSKMVSVSGNPIQRLGAYMLEALVARISSSGSIIYKSLKCKEPITATSKELLSHMHVLYEICPYLKFGYMSANGVIAEALKNESEVHIIDFQINQGIQWMSLIQALAGKPGGPPKIRITGFDDSTSAYARGGGLGIVGERLSKLAQSYNVAFEFHAIGASPSEVRLEDLELRRGEAIAVNFAMMLHHVPDEDVHGGKNHRDRLVRLAKCLSPKVVTLVEQESNTNELPFFARFVETMNYYFAVFESIDVALPRERRERINVEQHCLAREVVNLVACEGAERVERHEVLKKWRSRFTMAGFTPYPLSSYINCSIQNLLENYQGHYTLQEKDGALYLGWMNQPLITSSAWK