AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049197


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049197.t1 MTR_3g098140 82.424 165 15 2 1 160 1 156 9.02e-92 265
MS.gene049197.t1 MTR_3g098160 80.380 158 29 2 1 157 1 157 2.31e-90 262
MS.gene049197.t1 MTR_3g098170 77.019 161 35 2 1 160 1 160 6.05e-87 253
MS.gene049197.t1 MTR_3g098130 73.006 163 41 2 1 160 1 163 1.19e-85 250
MS.gene049197.t1 MTR_3g098130 73.006 163 41 2 1 160 39 201 1.77e-85 251
MS.gene049197.t1 MTR_3g098180 74.194 155 38 2 8 160 4 158 5.50e-81 238
MS.gene049197.t1 MTR_3g098120 64.375 160 51 1 1 160 43 196 9.99e-72 216
MS.gene049197.t1 MTR_3g098100 64.286 154 51 3 9 159 2 154 1.34e-65 199
MS.gene049197.t1 MTR_3g098160 79.825 114 21 2 1 113 1 113 1.02e-60 186
MS.gene049197.t1 MTR_3g098170 75.214 117 27 2 1 116 1 116 6.90e-57 176
MS.gene049197.t1 MTR_5g069640 38.333 120 72 1 34 153 37 154 4.80e-23 90.9
MS.gene049197.t1 MTR_8g032450 31.532 111 76 0 43 153 70 180 2.69e-11 60.5
MS.gene049197.t1 MTR_8g433000 31.532 111 76 0 43 153 70 180 2.69e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049197.t1 AT1G04290 59.868 152 57 2 9 157 2 152 1.54e-58 181
MS.gene049197.t1 AT1G04290 55.556 108 44 2 9 113 2 108 1.60e-33 116
MS.gene049197.t1 AT2G29590 37.903 124 71 3 32 153 32 151 2.49e-20 83.6

Find 31 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTACAAAGGGAATATATT+TGG 0.194020 3.3:+80776888 None:intergenic
GCTGGATTTCCGTGGAATTC+TGG 0.244104 3.3:-80777072 MS.gene049197:CDS
CTAATTAATTCGCAGAATTC+AGG 0.262630 3.3:-80777162 MS.gene049197:intron
AGCAGAAGCTTCAACAGATT+TGG 0.268181 3.3:-80777404 MS.gene049197:CDS
CTGTTGTAAGCGTGGAGTTC+AGG 0.335840 3.3:-80776951 MS.gene049197:CDS
AACCTTGCGCGGCCTACAAT+TGG 0.378951 3.3:-80777299 MS.gene049197:CDS
GAAGAGAGGCTTCTTAGTCT+TGG 0.391983 3.3:+80776030 None:intergenic
AGAAACTGCGAAAATTTAAC+TGG 0.399951 3.3:-80776124 MS.gene049197:CDS
GCAGCCGCAATTCCAGCAGC+TGG 0.448321 3.3:-80777090 MS.gene049197:CDS
GCATGAAATATTGATCTCAA+TGG 0.461406 3.3:+80777044 None:intergenic
GTTCAATGAGATCCAATTGT+AGG 0.486411 3.3:+80777287 None:intergenic
ATGAAGAATGTGAAGATCAA+TGG 0.502380 3.3:-80776097 MS.gene049197:CDS
TTCCACGGAAATCCAGCTGC+TGG 0.509333 3.3:+80777078 None:intergenic
CAATTGGATCTCATTGAACC+TGG 0.559460 3.3:-80777283 MS.gene049197:CDS
CGGTGGTGCTATAACTACTC+TGG 0.559606 3.3:-80777127 MS.gene049197:CDS
ATCCAATTGTAGGCCGCGCA+AGG 0.564575 3.3:+80777297 None:intergenic
GGTAGATGATGAATTGCCTC+AGG 0.574041 3.3:-80777338 MS.gene049197:CDS
GGCTGTAGCTGTTGTAAGCG+TGG 0.577050 3.3:-80776959 MS.gene049197:CDS
TTGGAACATCTAACCTTGCG+CGG 0.581241 3.3:-80777310 MS.gene049197:CDS
ATTGAGAAGACGACACGACC+AGG 0.583796 3.3:+80777265 None:intergenic
TTCCAGCAGCTGGATTTCCG+TGG 0.587898 3.3:-80777080 MS.gene049197:CDS
GAAAAGAGAAGCATCGTCAA+CGG 0.595530 3.3:-80777359 MS.gene049197:CDS
TGGTGCTATAACTACTCTGG+TGG 0.622116 3.3:-80777124 MS.gene049197:CDS
ATGGAAACTCCAGAATTCCA+CGG 0.622816 3.3:+80777063 None:intergenic
AAATCCAGCTGCTGGAATTG+CGG 0.627583 3.3:+80777086 None:intergenic
AATTCAGGTAAATACTTGCA+CGG 0.632650 3.3:-80777147 MS.gene049197:CDS
ACTACTCTGGTGGATATCGT+AGG 0.639860 3.3:-80777114 MS.gene049197:CDS
AAGAATGTGAAGATCAATGG+CGG 0.680804 3.3:-80776094 MS.gene049197:CDS
AGGATACGAACGAGTAAACG+TGG 0.698265 3.3:+80777232 None:intergenic
TCAGGTAAATACTTGCACGG+TGG 0.802740 3.3:-80777144 MS.gene049197:CDS
ATACGAACGAGTAAACGTGG+AGG 0.803457 3.3:+80777235 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAAATAAAAAATAAA+AGG - chr3.3:80776986-80777005 MS.gene049197:CDS 0.0%
!! AATAAAAAATAAAAGGAAAA+AGG - chr3.3:80776993-80777012 MS.gene049197:CDS 10.0%
!!! AAAAAGTGATTTTTTTTAAT+AGG + chr3.3:80776822-80776841 None:intergenic 10.0%
!!! AAAAGTGATTTTTTTTAATA+GGG + chr3.3:80776821-80776840 None:intergenic 10.0%
!! AAACAAACAATAGATTAAAT+GGG - chr3.3:80777129-80777148 MS.gene049197:CDS 15.0%
!!! AAAGTGATTTTTTTTAATAG+GGG + chr3.3:80776820-80776839 None:intergenic 15.0%
!! AAAATAAAAGGAAAAAGGAA+TGG - chr3.3:80776998-80777017 MS.gene049197:CDS 20.0%
!! CAAACAAACAATAGATTAAA+TGG - chr3.3:80777128-80777147 MS.gene049197:CDS 20.0%
!! GAGAAATAATGAATGTTAAA+TGG + chr3.3:80776686-80776705 None:intergenic 20.0%
!! GCGAATTAATTAGTTAATAT+TGG + chr3.3:80776286-80776305 None:intergenic 20.0%
!!! AAGTGATTTTTTTTAATAGG+GGG + chr3.3:80776819-80776838 None:intergenic 20.0%
!!! CAATCTTGTAATTTTCTTAT+TGG - chr3.3:80776756-80776775 MS.gene049197:intron 20.0%
!!! GGAATATTTTATTTGAATCA+TGG + chr3.3:80776665-80776684 None:intergenic 20.0%
!!! TTTAACATTCATTATTTCTC+AGG - chr3.3:80776686-80776705 MS.gene049197:intron 20.0%
!!! TTTCACATTTTACTTACAAA+GGG + chr3.3:80776581-80776600 None:intergenic 20.0%
! AAATTTCAGAAGCTTCTTTA+AGG + chr3.3:80777243-80777262 None:intergenic 25.0%
! AAGAAAATGAAGAATAGTGT+AGG + chr3.3:80776246-80776265 None:intergenic 25.0%
! AATTTCAGAAGCTTCTTTAA+GGG + chr3.3:80777242-80777261 None:intergenic 25.0%
! ACTTACAAAGGGAATATATT+TGG + chr3.3:80776570-80776589 None:intergenic 25.0%
!! CTATTGTTTGTTTGTGTTTT+TGG + chr3.3:80777122-80777141 None:intergenic 25.0%
!! GTTTCACATTTTACTTACAA+AGG + chr3.3:80776582-80776601 None:intergenic 25.0%
!! TAATTCAAAATTTTCCTACG+TGG + chr3.3:80776930-80776949 None:intergenic 25.0%
AATTCAGGTAAATACTTGCA+CGG - chr3.3:80776308-80776327 MS.gene049197:intron 30.0%
ACTCAGATCTAACAATTTAG+TGG - chr3.3:80777176-80777195 MS.gene049197:intron 30.0%
AGAAACTGCGAAAATTTAAC+TGG - chr3.3:80777331-80777350 MS.gene049197:CDS 30.0%
ATGAAGAATGTGAAGATCAA+TGG - chr3.3:80777358-80777377 MS.gene049197:CDS 30.0%
CTAATTAATTCGCAGAATTC+AGG - chr3.3:80776293-80776312 MS.gene049197:intron 30.0%
GCATGAAATATTGATCTCAA+TGG + chr3.3:80776414-80776433 None:intergenic 30.0%
GTTGAAGTGACTTTAATATG+AGG + chr3.3:80777095-80777114 None:intergenic 30.0%
TAATTCATAAAATACACGCG+TGG + chr3.3:80776969-80776988 None:intergenic 30.0%
! TAGAGTTTTACGAATAGGAA+AGG - chr3.3:80776475-80776494 MS.gene049197:intron 30.0%
! TTCATTATTTCTCAGGTGAT+AGG - chr3.3:80776693-80776712 MS.gene049197:intron 30.0%
!! AGGGTTTCTATGTTTGATTT+TGG + chr3.3:80777223-80777242 None:intergenic 30.0%
AAATGTGAAACTGAGACCAA+AGG - chr3.3:80776591-80776610 MS.gene049197:intron 35.0%
AAGAATGTGAAGATCAATGG+CGG - chr3.3:80777361-80777380 MS.gene049197:CDS 35.0%
ATACATCTTCTACTGTCCTT+TGG + chr3.3:80776610-80776629 None:intergenic 35.0%
CAGATCTAACAATTTAGTGG+TGG - chr3.3:80777179-80777198 MS.gene049197:intron 35.0%
GAAGATGTATGAACTTTCTG+AGG - chr3.3:80776620-80776639 MS.gene049197:intron 35.0%
GTGACTTTAATATGAGGAGA+TGG + chr3.3:80777089-80777108 None:intergenic 35.0%
GTTCAATGAGATCCAATTGT+AGG + chr3.3:80776171-80776190 None:intergenic 35.0%
TAATATGAGGAGATGGAAGA+AGG + chr3.3:80777082-80777101 None:intergenic 35.0%
TATTTCTCAGGTGATAGGAT+CGG - chr3.3:80776698-80776717 MS.gene049197:intron 35.0%
TTGACTCAGTCAAAATCAAG+GGG + chr3.3:80776882-80776901 None:intergenic 35.0%
! GATAGGATCGGATTTTTATC+AGG - chr3.3:80776710-80776729 MS.gene049197:intron 35.0%
! GATGCTAGAGTTTTACGAAT+AGG - chr3.3:80776470-80776489 MS.gene049197:intron 35.0%
! GTTGACTCAGTCAAAATCAA+GGG + chr3.3:80776883-80776902 None:intergenic 35.0%
! TTCTGTTTCTTTCTCTGAGT+GGG + chr3.3:80777294-80777313 None:intergenic 35.0%
! TTTCTGTTTCTTTCTCTGAG+TGG + chr3.3:80777295-80777314 None:intergenic 35.0%
!! TGTTAGCTTAAACGCACTTT+TGG - chr3.3:80776793-80776812 MS.gene049197:intron 35.0%
!!! GTTTGTTTGTGTTTTTGGTG+TGG + chr3.3:80777117-80777136 None:intergenic 35.0%
AGATGTTCCAAAAACTCCTG+AGG + chr3.3:80776136-80776155 None:intergenic 40.0%
AGCAGAAGCTTCAACAGATT+TGG - chr3.3:80776051-80776070 MS.gene049197:CDS 40.0%
ATGGAAACTCCAGAATTCCA+CGG + chr3.3:80776395-80776414 None:intergenic 40.0%
ATGGAAGAAGGATAAGGAGA+TGG + chr3.3:80777070-80777089 None:intergenic 40.0%
CAATTGGATCTCATTGAACC+TGG - chr3.3:80776172-80776191 MS.gene049197:intron 40.0%
GAGTTCAGGAAGAAAAAGAC+TGG - chr3.3:80776518-80776537 MS.gene049197:intron 40.0%
TGACTCAGTCAAAATCAAGG+GGG + chr3.3:80776881-80776900 None:intergenic 40.0%
TGCAAAAGGGGGAAACTTAA+GGG + chr3.3:80776848-80776867 None:intergenic 40.0%
TTGCAAAAGGGGGAAACTTA+AGG + chr3.3:80776849-80776868 None:intergenic 40.0%
! ACTGGACAAGTTTTTGCTCA+AGG - chr3.3:80776536-80776555 MS.gene049197:intron 40.0%
! CTGGACAAGTTTTTGCTCAA+GGG - chr3.3:80776537-80776556 MS.gene049197:intron 40.0%
! TGAATTGCCTCAGGAGTTTT+TGG - chr3.3:80776126-80776145 MS.gene049197:CDS 40.0%
!! CGTTGACTCAGTCAAAATCA+AGG + chr3.3:80776884-80776903 None:intergenic 40.0%
!! GAAAAGAGAAGCATCGTCAA+CGG - chr3.3:80776096-80776115 MS.gene049197:CDS 40.0%
AAATCCAGCTGCTGGAATTG+CGG + chr3.3:80776372-80776391 None:intergenic 45.0%
AGGATACGAACGAGTAAACG+TGG + chr3.3:80776226-80776245 None:intergenic 45.0%
ATACGAACGAGTAAACGTGG+AGG + chr3.3:80776223-80776242 None:intergenic 45.0%
GAAGAAGGATAAGGAGATGG+AGG + chr3.3:80777067-80777086 None:intergenic 45.0%
GACTCAGTCAAAATCAAGGG+GGG + chr3.3:80776880-80776899 None:intergenic 45.0%
GAGGAGATGGAAGAAGGATA+AGG + chr3.3:80777076-80777095 None:intergenic 45.0%
GCAAAAGGGGGAAACTTAAG+GGG + chr3.3:80776847-80776866 None:intergenic 45.0%
GGTAGATGATGAATTGCCTC+AGG - chr3.3:80776117-80776136 MS.gene049197:CDS 45.0%
TCAGGTAAATACTTGCACGG+TGG - chr3.3:80776311-80776330 MS.gene049197:intron 45.0%
TTGGAACATCTAACCTTGCG+CGG - chr3.3:80776145-80776164 MS.gene049197:CDS 45.0%
! ACTACTCTGGTGGATATCGT+AGG - chr3.3:80776341-80776360 MS.gene049197:intron 45.0%
!! GATAAGGAGATGGAGGTGTT+GGG + chr3.3:80777060-80777079 None:intergenic 45.0%
!! TGGTGCTATAACTACTCTGG+TGG - chr3.3:80776331-80776350 MS.gene049197:intron 45.0%
ATTGAGAAGACGACACGACC+AGG + chr3.3:80776193-80776212 None:intergenic 50.0%
CAAAATCAAGGGGGGCTTGA+AGG + chr3.3:80776872-80776891 None:intergenic 50.0%
CTGTTGTAAGCGTGGAGTTC+AGG - chr3.3:80776504-80776523 MS.gene049197:intron 50.0%
GCTGGATTTCCGTGGAATTC+TGG - chr3.3:80776383-80776402 MS.gene049197:intron 50.0%
GGCTTGAAGGTTGCAAAAGG+GGG + chr3.3:80776859-80776878 None:intergenic 50.0%
GGGCTTGAAGGTTGCAAAAG+GGG + chr3.3:80776860-80776879 None:intergenic 50.0%
GGGGCTTGAAGGTTGCAAAA+GGG + chr3.3:80776861-80776880 None:intergenic 50.0%
TGACTGAGTCAACGCTTACG+TGG - chr3.3:80776890-80776909 MS.gene049197:intron 50.0%
! GGATAAGGAGATGGAGGTGT+TGG + chr3.3:80777061-80777080 None:intergenic 50.0%
!! CGGTGGTGCTATAACTACTC+TGG - chr3.3:80776328-80776347 MS.gene049197:intron 50.0%
AACCTTGCGCGGCCTACAAT+TGG - chr3.3:80776156-80776175 MS.gene049197:CDS 55.0%
ACGCTTACGTGGCTCCTAGT+TGG - chr3.3:80776901-80776920 MS.gene049197:CDS 55.0%
ATCCAATTGTAGGCCGCGCA+AGG + chr3.3:80776161-80776180 None:intergenic 55.0%
GGCTGTAGCTGTTGTAAGCG+TGG - chr3.3:80776496-80776515 MS.gene049197:intron 55.0%
GGGGGCTTGAAGGTTGCAAA+AGG + chr3.3:80776862-80776881 None:intergenic 55.0%
TTCCACGGAAATCCAGCTGC+TGG + chr3.3:80776380-80776399 None:intergenic 55.0%
TTCCAGCAGCTGGATTTCCG+TGG - chr3.3:80776375-80776394 MS.gene049197:intron 55.0%
TTCCTACGTGGCGTCCAACT+AGG + chr3.3:80776918-80776937 None:intergenic 55.0%
CTCCTAGTTGGACGCCACGT+AGG - chr3.3:80776913-80776932 MS.gene049197:CDS 60.0%
GCAGCCGCAATTCCAGCAGC+TGG - chr3.3:80776365-80776384 MS.gene049197:intron 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 80776043 80777434 80776043 ID=MS.gene049197
chr3.3 mRNA 80776043 80777434 80776043 ID=MS.gene049197.t1;Parent=MS.gene049197
chr3.3 exon 80777243 80777434 80777243 ID=MS.gene049197.t1.exon1;Parent=MS.gene049197.t1
chr3.3 CDS 80777243 80777434 80777243 ID=cds.MS.gene049197.t1;Parent=MS.gene049197.t1
chr3.3 exon 80776894 80777169 80776894 ID=MS.gene049197.t1.exon2;Parent=MS.gene049197.t1
chr3.3 CDS 80776894 80777169 80776894 ID=cds.MS.gene049197.t1;Parent=MS.gene049197.t1
chr3.3 exon 80776043 80776177 80776043 ID=MS.gene049197.t1.exon3;Parent=MS.gene049197.t1
chr3.3 CDS 80776043 80776177 80776043 ID=cds.MS.gene049197.t1;Parent=MS.gene049197.t1
Gene Sequence

>MS.gene049197

ATGGGAAAAGCAGAAGCTTCAACAGATTTGGAATCAGTGAAGAGTTATTTCGAGAAAAGAGAAGCATCGTCAACGGTAGATGATGAATTGCCTCAGGAGTTTTTGGAACATCTAACCTTGCGCGGCCTACAATTGGATCTCATTGAACCTGGTCGTGTCGTCTTCTCAATGAACATACCTCCACGTTTACTCGTTCGTATCCTACACTATTCTTCATTTTCTTCTGCTTTGTTTTTTAAACCAATATTAACTAATTAATTCGCAGAATTCAGGTAAATACTTGCACGGTGGTGCTATAACTACTCTGGTGGATATCGTAGGCGCAGCCGCAATTCCAGCAGCTGGATTTCCGTGGAATTCTGGAGTTTCCATTGAGATCAATATTTCATGCTTTGATGCTGCTTACGTCAACGAAGAGATTGAGATTGATGCTAGAGTTTTACGAATAGGAAAGGCTGTAGCTGTTGTAAGCGTGGAGTTCAGGAAGAAAAAGACTGGACAAGTTTTTGCTCAAGGGCGTCATACCAAATATATTCCCTTTGTAAGTAAAATGTGAAACTGAGACCAAAGGACAGTAGAAGATGTATGAACTTTCTGAGGTAGAAAACTCTCTATTCTACCATGATTCAAATAAAATATTCCATTTAACATTCATTATTTCTCAGGTGATAGGATCGGATTTTTATCAGGTTTTGTTTGTGTTTCATATATATCAATCTTGTAATTTTCTTATTGGAAAGTACTAACACATGTTAGCTTAAACGCACTTTTGGCCCCCTATTAAAAAAAATCACTTTTTAGCCCCTTAAGTTTCCCCCTTTTGCAACCTTCAAGCCCCCCTTGATTTTGACTGAGTCAACGCTTACGTGGCTCCTAGTTGGACGCCACGTAGGAAAATTTTGAATTAAAAACAAATGAAAATGCCACGCGTGTATTTTATGAATTAAAAAAATAAAAAATAAAAGGAAAAAGGAATGGCACGTGAGATCATCATGTTCTTGTTCATCTTCTTCACCCAACACCTCCATCTCCTTATCCTTCTTCCATCTCCTCATATTAAAGTCACTTCAACCACACCAAAAACACAAACAAACAATAGATTAAATGGGAAGAACACTCAAAATCAAACATAAACTCAGATCTAACAATTTAGTGGTGGAGTTTCTACAAAATTTTACCAAAATCAAACATAGAAACCCTTAAAGAAGCTTCTGAAATTTGAGAGTTATAAGAAACTGCGAAAATTTTCCCACTCAGAGAAAGAAACAGAAATCTCGAAGAAGTTATAAGAAACTGCGAAAATTTAACTGGCGAGATGAAGAATGTGAAGATCAATGGCGGCGAGATCGAGAAAGAGAAGAAGTTCTTTCGTATTCAAGTTTCCAAGACTAA

Protein sequence

>MS.gene049197.t1

MGKAEASTDLESVKSYFEKREASSTVDDELPQEFLEHLTLRGLQLDLIEPGRVVFSMNIPPRLLNSGKYLHGGAITTLVDIVGAAAIPAAGFPWNSGVSIEINISCFDAAYVNEEIEIDARVLRIGKAVAVVSVEFRKKKTGQVFAQGRHTKYIPFRKKQKSRRSYKKLRKFNWRDEECEDQWRRDREREEVLSYSSFQD