AlfalfaGEDB | MS.gene64234

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64234.t1	XP_024633138.1	97.7	86	2	0	1	86	43	128	2.10E-19	104.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64234.t1	G7IR18	97.7	86	2	0	1	86	43	128	1.5e-19	104.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050363	MS.gene64234	0.815123	1.09E-51	-1.69E-46
MS.gene050883	MS.gene64234	0.806167	9.38E-50	-1.69E-46
MS.gene053549	MS.gene64234	0.82596	3.52E-54	-1.69E-46
MS.gene055551	MS.gene64234	0.80156	8.50E-49	-1.69E-46
MS.gene056408	MS.gene64234	0.812846	3.45E-51	-1.69E-46
MS.gene058628	MS.gene64234	0.810843	9.42E-51	-1.69E-46
MS.gene058629	MS.gene64234	0.815011	1.15E-51	-1.69E-46
MS.gene058630	MS.gene64234	0.808567	2.91E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64234.t1	MTR_2g075550	93.023	86	6	0	1	86	43	128	5.36e-51	156

Find 31 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTCCTCCTGGTCTAACTAT+TGG	0.312494	2.1:-24032814	None:intergenic
CTACCCGTTTGTTCACCTAT+TGG	0.318886	2.1:-24032874	None:intergenic
TAGCACAGGAAGCGAAGGTT+TGG	0.359713	2.1:+24032966	MS.gene64234:CDS
GTTAGACCAGGAGGAGGATT+GGG	0.360690	2.1:+24032820	MS.gene64234:CDS
AGCACAGGAAGCGAAGGTTT+GGG	0.387244	2.1:+24032967	MS.gene64234:CDS
ACGATCAATCGACGACCAAT+AGG	0.406349	2.1:+24032859	MS.gene64234:CDS
AGCTGAGGATTCTCCATTTG+CGG	0.436685	2.1:-24032997	None:intergenic
TAGCACAGGAAGCGAAGGTT+TGG	0.442530	2.1:+24032894	MS.gene64234:CDS
TACCCATTGGTTCACCATCA+TGG	0.447609	2.1:-24032945	None:intergenic
TGGGAATTGATCGTCGTTTG+CGG	0.453040	2.1:-24032925	None:intergenic
TCGCTTCCTGTGCTACCCAT+TGG	0.453513	2.1:-24032958	None:intergenic
AGTTAGACCAGGAGGAGGAT+TGG	0.473714	2.1:+24032819	MS.gene64234:CDS
ACCCATTGGTTCACCATCAT+GGG	0.505198	2.1:-24032944	None:intergenic
GGTGGATTTGTTGGTAGCTG+AGG	0.509124	2.1:-24033012	None:intergenic
TCAGTCCCCAATCCTCCTCC+TGG	0.512490	2.1:-24032826	None:intergenic
ACGACCAATAGGTGAACAAA+CGG	0.523537	2.1:+24032870	MS.gene64234:CDS
TTTGGGGACTGATCCGCAAA+TGG	0.526958	2.1:+24032984	MS.gene64234:CDS
TTCCCATGATGGTGAACCAA+TGG	0.532368	2.1:+24032942	MS.gene64234:CDS
TCCCATGATGGTGAACCAAT+GGG	0.536615	2.1:+24032943	MS.gene64234:CDS
GGTGAACCAATGGGTAGCAC+AGG	0.545743	2.1:+24032952	MS.gene64234:CDS
GGTGAACAAACGGGTAGCAC+AGG	0.559451	2.1:+24032880	MS.gene64234:CDS
ACGACGATCAATTCCCATGA+TGG	0.561161	2.1:+24032931	MS.gene64234:CDS
CGACCAATAGGTGAACAAAC+GGG	0.567195	2.1:+24032871	MS.gene64234:CDS
CGTCGATTGATCGTCGATTG+CGG	0.572151	2.1:-24032850	None:intergenic
GCACAGGAAGCGAAGGTTTG+GGG	0.608095	2.1:+24032968	MS.gene64234:CDS
ACGGGTAGCACAGGAAGCGA+AGG	0.609094	2.1:+24032889	MS.gene64234:CDS
ATGGGTAGCACAGGAAGCGA+AGG	0.613165	2.1:+24032961	MS.gene64234:CDS
TTAGACCAGGAGGAGGATTG+GGG	0.626507	2.1:+24032821	MS.gene64234:CDS
CCAATAGTTAGACCAGGAGG+AGG	0.628698	2.1:+24032814	MS.gene64234:CDS
ATGAAACCAATAGTTAGACC+AGG	0.663167	2.1:+24032808	MS.gene64234:CDS
AAACCAATAGTTAGACCAGG+AGG	0.695638	2.1:+24032811	MS.gene64234:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCATCATCATCAGAATTTTT+TGG	-	chr2.1:24033036-24033055	None:intergenic	25.0%
!!!	GAATTTTTTGGTGGATTTGT+TGG	-	chr2.1:24033024-24033043	None:intergenic	30.0%
!!!	TCATCATCAGAATTTTTTGG+TGG	-	chr2.1:24033033-24033052	None:intergenic	30.0%
	ATGAAACCAATAGTTAGACC+AGG	+	chr2.1:24032808-24032827	MS.gene64234:CDS	35.0%
	AAACCAATAGTTAGACCAGG+AGG	+	chr2.1:24032811-24032830	MS.gene64234:CDS	40.0%
	ACGACCAATAGGTGAACAAA+CGG	+	chr2.1:24032870-24032889	MS.gene64234:CDS	40.0%
	ACGACGATCAATTCCCATGA+TGG	+	chr2.1:24032931-24032950	MS.gene64234:CDS	45.0%
	ACGATCAATCGACGACCAAT+AGG	+	chr2.1:24032859-24032878	MS.gene64234:CDS	45.0%
	AGCTGAGGATTCTCCATTTG+CGG	-	chr2.1:24033000-24033019	None:intergenic	45.0%
	CGACCAATAGGTGAACAAAC+GGG	+	chr2.1:24032871-24032890	MS.gene64234:CDS	45.0%
	CTACCCGTTTGTTCACCTAT+TGG	-	chr2.1:24032877-24032896	None:intergenic	45.0%
	TGGGAATTGATCGTCGTTTG+CGG	-	chr2.1:24032928-24032947	None:intergenic	45.0%
!	TCCCATGATGGTGAACCAAT+GGG	+	chr2.1:24032943-24032962	MS.gene64234:CDS	45.0%
!	TTCCCATGATGGTGAACCAA+TGG	+	chr2.1:24032942-24032961	MS.gene64234:CDS	45.0%
!!	ACCCATTGGTTCACCATCAT+GGG	-	chr2.1:24032947-24032966	None:intergenic	45.0%
!!	TACCCATTGGTTCACCATCA+TGG	-	chr2.1:24032948-24032967	None:intergenic	45.0%
	AGCACAGGAAGCGAAGGTTT+GGG	+	chr2.1:24032895-24032914	MS.gene64234:CDS	50.0%
	AGTTAGACCAGGAGGAGGAT+TGG	+	chr2.1:24032819-24032838	MS.gene64234:CDS	50.0%
	CCAATAGTTAGACCAGGAGG+AGG	+	chr2.1:24032814-24032833	MS.gene64234:CDS	50.0%
	CCTCCTCCTGGTCTAACTAT+TGG	-	chr2.1:24032817-24032836	None:intergenic	50.0%
	GTTAGACCAGGAGGAGGATT+GGG	+	chr2.1:24032820-24032839	MS.gene64234:CDS	50.0%
	TAGCACAGGAAGCGAAGGTT+TGG	+	chr2.1:24032894-24032913	MS.gene64234:CDS	50.0%
	TTAGACCAGGAGGAGGATTG+GGG	+	chr2.1:24032821-24032840	MS.gene64234:CDS	50.0%
	TTTGGGGACTGATCCGCAAA+TGG	+	chr2.1:24032984-24033003	MS.gene64234:CDS	50.0%
!	CGTCGATTGATCGTCGATTG+CGG	-	chr2.1:24032853-24032872	None:intergenic	50.0%
!!	GGTGGATTTGTTGGTAGCTG+AGG	-	chr2.1:24033015-24033034	None:intergenic	50.0%
	ATGGGTAGCACAGGAAGCGA+AGG	+	chr2.1:24032961-24032980	MS.gene64234:CDS	55.0%
	GCACAGGAAGCGAAGGTTTG+GGG	+	chr2.1:24032896-24032915	MS.gene64234:CDS	55.0%
	GGTGAACAAACGGGTAGCAC+AGG	+	chr2.1:24032880-24032899	MS.gene64234:CDS	55.0%
	GGTGAACCAATGGGTAGCAC+AGG	+	chr2.1:24032952-24032971	MS.gene64234:CDS	55.0%
	TCGCTTCCTGTGCTACCCAT+TGG	-	chr2.1:24032961-24032980	None:intergenic	55.0%
	ACGGGTAGCACAGGAAGCGA+AGG	+	chr2.1:24032889-24032908	MS.gene64234:CDS	60.0%
	TCAGTCCCCAATCCTCCTCC+TGG	-	chr2.1:24032829-24032848	None:intergenic	60.0%

Gene Sequence	>MS.gene64234 ATGAAACCAATAGTTAGACCAGGAGGAGGATTGGGGACTGATCCGCAATCGACGATCAATCGACGACCAATAGGTGAACAAACGGGTAGCACAGGAAGCGAAGGTTTGGAGACTGATCCGCAAACGACGATCAATTCCCATGATGGTGAACCAATGGGTAGCACAGGAAGCGAAGGTTTGGGGACTGATCCGCAAATGGAGAATCCTCAGCTACCAACAAATCCACCAAAAAATTCTGATGATGATGAAGATCAACTTTGA
Protein sequence	>MS.gene64234.t1 MKPIVRPGGGLGTDPQSTINRRPIGEQTGSTGSEGLETDPQTTINSHDGEPMGSTGSEGLGTDPQMENPQLPTNPPKNSDDDEDQL

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	24032808	24033068	24032808	ID=MS.gene64234
chr2.1	mRNA	24032808	24033068	24032808	ID=MS.gene64234.t1;Parent=MS.gene64234
chr2.1	exon	24032808	24033068	24032808	ID=MS.gene64234.t1.exon1;Parent=MS.gene64234.t1
chr2.1	CDS	24032808	24033068	24032808	ID=cds.MS.gene64234.t1;Parent=MS.gene64234.t1

M. sativa cultivar XinJiangDaYe / MS.gene64234