AlfalfaGEDB | MS.gene64349

M. sativa cultivar XinJiangDaYe / MS.gene64349

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64349.t1	XP_003596225.2	93.5	155	10	0	1	155	1	155	1.10E-79	305.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64349.t1	G7IJC5	93.5	155	10	0	1	155	1	155	8.0e-80	305.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050559	MS.gene64349	0.800293	1.54E-48	-1.69E-46
MS.gene050807	MS.gene64349	0.807982	3.87E-50	-1.69E-46
MS.gene051279	MS.gene64349	0.805781	1.13E-49	-1.69E-46
MS.gene052239	MS.gene64349	-0.83694	6.97E-57	-1.69E-46
MS.gene055464	MS.gene64349	0.801389	9.21E-49	-1.69E-46
MS.gene057287	MS.gene64349	0.805106	1.57E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64349.t1	MTR_2g071790	93.548	155	10	0	1	155	1	155	7.88e-105	296
MS.gene64349.t1	MTR_2g071760	88.387	155	18	0	1	155	1	155	2.05e-96	275
MS.gene64349.t1	MTR_2g071750	86.452	155	21	0	1	155	1	155	3.87e-96	274
MS.gene64349.t1	MTR_2g071740	85.621	153	16	2	1	152	1	148	2.31e-89	257

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 40 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGGGTTGAGTGGGTGGATA+TGG	0.201977	2.4:+24509557	MS.gene64349:CDS
TGTGATGGTTGATCATTTAA+TGG	0.277636	2.4:+24509650	MS.gene64349:CDS
CCCAACTTGCATCATCAAAT+TGG	0.284176	2.4:-24509596	None:intergenic
TTGTGATGATGGGTTTGATA+TGG	0.302331	2.4:+24509674	MS.gene64349:CDS
TGGAGGAGGGACATGACTTT+GGG	0.323166	2.4:+24509538	MS.gene64349:CDS
AACATGCTTACAATTCCTTT+TGG	0.331685	2.4:+24509700	MS.gene64349:CDS
CATTTAATGGTTTGTGATGA+TGG	0.359988	2.4:+24509663	MS.gene64349:CDS
CCAATTTGATGATGCAAGTT+GGG	0.373282	2.4:+24509596	MS.gene64349:CDS
AAGAATATTATGATGAATCA+TGG	0.395210	2.4:+24509499	MS.gene64349:CDS
ATGGAGGAGGGACATGACTT+TGG	0.417476	2.4:+24509537	MS.gene64349:CDS
TGCTTGTGATGTGGAAATCA+AGG	0.457434	2.4:+24509314	MS.gene64349:CDS
GACATGACTTTGGGGTTGAG+TGG	0.467986	2.4:+24509547	MS.gene64349:CDS
TCCAATTTGATGATGCAAGT+TGG	0.473251	2.4:+24509595	MS.gene64349:CDS
CTATGCATGTTGAATTATCA+TGG	0.491083	2.4:-24509452	None:intergenic
ATTTAATGGTTTGTGATGAT+GGG	0.498358	2.4:+24509664	MS.gene64349:CDS
CTCATACCTATCTTGCCAAA+AGG	0.500774	2.4:-24509715	None:intergenic
ACATGACTTTGGGGTTGAGT+GGG	0.501007	2.4:+24509548	MS.gene64349:CDS
TGAAATTAGTGAAAGCAAGA+TGG	0.502049	2.4:+24509425	MS.gene64349:CDS
TGATCTTCTCACGTCACTGA+TGG	0.502867	2.4:+24509338	MS.gene64349:CDS
TGGCAAGATAGGTATGAGAT+GGG	0.517786	2.4:+24509720	MS.gene64349:CDS
TTGGCAAGATAGGTATGAGA+TGG	0.518652	2.4:+24509719	MS.gene64349:CDS
TGACTTTGGGGTTGAGTGGG+TGG	0.527133	2.4:+24509551	MS.gene64349:CDS
TATGATGAATCATGGAACAT+AGG	0.530061	2.4:+24509507	MS.gene64349:CDS
CATGATAATTCAACATGCAT+AGG	0.547359	2.4:+24509453	MS.gene64349:CDS
GAAATTAGTGAAAGCAAGAT+GGG	0.562708	2.4:+24509426	MS.gene64349:CDS
ATGGAACATAGGACAAGTGA+TGG	0.569406	2.4:+24509518	MS.gene64349:CDS
GGAGGAGGGACATGACTTTG+GGG	0.584089	2.4:+24509539	MS.gene64349:CDS
TGGGAGGCAATTGAGTCTTG+TGG	0.585282	2.4:+24509615	MS.gene64349:CDS
CATAGGACAAGTGATGGAGG+AGG	0.595117	2.4:+24509524	MS.gene64349:CDS
ACTTGCATCATCAAATTGGA+AGG	0.605601	2.4:-24509592	None:intergenic
TGGAGATGAGAAAGATGTGA+TGG	0.610494	2.4:+24509635	MS.gene64349:CDS
GGCAAGATAGGTATGAGATG+GGG	0.613154	2.4:+24509721	MS.gene64349:CDS
GCTTGTGATGTGGAAATCAA+GGG	0.616324	2.4:+24509315	MS.gene64349:CDS
AGATAGGTATGAGATGGGGT+TGG	0.636490	2.4:+24509725	MS.gene64349:CDS
ATTTGATGATGCAAGTTGGG+AGG	0.639497	2.4:+24509599	MS.gene64349:CDS
GAACATAGGACAAGTGATGG+AGG	0.641704	2.4:+24509521	MS.gene64349:CDS
TCATCATTACTCTCATCACA+TGG	0.648258	2.4:-24509369	None:intergenic
GTGATGAGAGTAATGATGAA+AGG	0.650915	2.4:+24509373	MS.gene64349:CDS
ATAGGACAAGTGATGGAGGA+GGG	0.671083	2.4:+24509525	MS.gene64349:CDS
ATTAGTGAAAGCAAGATGGG+TGG	0.695184	2.4:+24509429	MS.gene64349:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAATATTATGATGAATCA+TGG	+	chr2.4:24509499-24509518	MS.gene64349:CDS	20.0%
!!!	TAGTTTAATAAGATCTTTTG+AGG	+	chr2.4:24509401-24509420	MS.gene64349:CDS	20.0%
!!	ATTTAATGGTTTGTGATGAT+GGG	+	chr2.4:24509664-24509683	MS.gene64349:CDS	25.0%
	CATGATAATTCAACATGCAT+AGG	+	chr2.4:24509453-24509472	MS.gene64349:CDS	30.0%
	CTATGCATGTTGAATTATCA+TGG	-	chr2.4:24509455-24509474	None:intergenic	30.0%
	GAAATTAGTGAAAGCAAGAT+GGG	+	chr2.4:24509426-24509445	MS.gene64349:CDS	30.0%
	TATGATGAATCATGGAACAT+AGG	+	chr2.4:24509507-24509526	MS.gene64349:CDS	30.0%
	TGAAATTAGTGAAAGCAAGA+TGG	+	chr2.4:24509425-24509444	MS.gene64349:CDS	30.0%
	TGTGATGGTTGATCATTTAA+TGG	+	chr2.4:24509650-24509669	MS.gene64349:CDS	30.0%
!	AACATGCTTACAATTCCTTT+TGG	+	chr2.4:24509700-24509719	MS.gene64349:CDS	30.0%
!	CATTTAATGGTTTGTGATGA+TGG	+	chr2.4:24509663-24509682	MS.gene64349:CDS	30.0%
	ACTTGCATCATCAAATTGGA+AGG	-	chr2.4:24509595-24509614	None:intergenic	35.0%
	GTGATGAGAGTAATGATGAA+AGG	+	chr2.4:24509373-24509392	MS.gene64349:CDS	35.0%
	TCATCATTACTCTCATCACA+TGG	-	chr2.4:24509372-24509391	None:intergenic	35.0%
!	ACAATTCCTTTTGGCAAGAT+AGG	+	chr2.4:24509709-24509728	MS.gene64349:CDS	35.0%
!	TGATACTTTTGCTTGTGATG+TGG	+	chr2.4:24509305-24509324	MS.gene64349:CDS	35.0%
!!	CCAATTTGATGATGCAAGTT+GGG	+	chr2.4:24509596-24509615	MS.gene64349:CDS	35.0%
!!	TCCAATTTGATGATGCAAGT+TGG	+	chr2.4:24509595-24509614	MS.gene64349:CDS	35.0%
!!	TTGTGATGATGGGTTTGATA+TGG	+	chr2.4:24509674-24509693	MS.gene64349:CDS	35.0%
	ATGGAACATAGGACAAGTGA+TGG	+	chr2.4:24509518-24509537	MS.gene64349:CDS	40.0%
	ATTAGTGAAAGCAAGATGGG+TGG	+	chr2.4:24509429-24509448	MS.gene64349:CDS	40.0%
	CCCAACTTGCATCATCAAAT+TGG	-	chr2.4:24509599-24509618	None:intergenic	40.0%
	CTCATACCTATCTTGCCAAA+AGG	-	chr2.4:24509718-24509737	None:intergenic	40.0%
	GCTTGTGATGTGGAAATCAA+GGG	+	chr2.4:24509315-24509334	MS.gene64349:CDS	40.0%
	TGCTTGTGATGTGGAAATCA+AGG	+	chr2.4:24509314-24509333	MS.gene64349:CDS	40.0%
	TGGAGATGAGAAAGATGTGA+TGG	+	chr2.4:24509635-24509654	MS.gene64349:CDS	40.0%
	TGGCAAGATAGGTATGAGAT+GGG	+	chr2.4:24509720-24509739	MS.gene64349:CDS	40.0%
	TTGGCAAGATAGGTATGAGA+TGG	+	chr2.4:24509719-24509738	MS.gene64349:CDS	40.0%
!!	ATTTGATGATGCAAGTTGGG+AGG	+	chr2.4:24509599-24509618	MS.gene64349:CDS	40.0%
	AGATAGGTATGAGATGGGGT+TGG	+	chr2.4:24509725-24509744	MS.gene64349:CDS	45.0%
	GAACATAGGACAAGTGATGG+AGG	+	chr2.4:24509521-24509540	MS.gene64349:CDS	45.0%
	GGCAAGATAGGTATGAGATG+GGG	+	chr2.4:24509721-24509740	MS.gene64349:CDS	45.0%
	TGATCTTCTCACGTCACTGA+TGG	+	chr2.4:24509338-24509357	MS.gene64349:CDS	45.0%
!	ATAGGACAAGTGATGGAGGA+GGG	+	chr2.4:24509525-24509544	MS.gene64349:CDS	45.0%
!!	ACATGACTTTGGGGTTGAGT+GGG	+	chr2.4:24509548-24509567	MS.gene64349:CDS	45.0%
	ATGGAGGAGGGACATGACTT+TGG	+	chr2.4:24509537-24509556	MS.gene64349:CDS	50.0%
	TGGAGGAGGGACATGACTTT+GGG	+	chr2.4:24509538-24509557	MS.gene64349:CDS	50.0%
	TGGGAGGCAATTGAGTCTTG+TGG	+	chr2.4:24509615-24509634	MS.gene64349:CDS	50.0%
!	CATAGGACAAGTGATGGAGG+AGG	+	chr2.4:24509524-24509543	MS.gene64349:CDS	50.0%
!!	GACATGACTTTGGGGTTGAG+TGG	+	chr2.4:24509547-24509566	MS.gene64349:CDS	50.0%
	GGAGGAGGGACATGACTTTG+GGG	+	chr2.4:24509539-24509558	MS.gene64349:CDS	55.0%
	TGGGGTTGAGTGGGTGGATA+TGG	+	chr2.4:24509557-24509576	MS.gene64349:CDS	55.0%
!!	TGACTTTGGGGTTGAGTGGG+TGG	+	chr2.4:24509551-24509570	MS.gene64349:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	24509288	24509755	24509288	ID=MS.gene64349
chr2.4	mRNA	24509288	24509755	24509288	ID=MS.gene64349.t1;Parent=MS.gene64349
chr2.4	exon	24509288	24509755	24509288	ID=MS.gene64349.t1.exon1;Parent=MS.gene64349.t1
chr2.4	CDS	24509288	24509755	24509288	ID=cds.MS.gene64349.t1;Parent=MS.gene64349.t1

Gene Sequence

>MS.gene64349

ATGGCTTCTCAAGTTTGTGATACTTTTGCTTGTGATGTGGAAATCAAGGGTGATCTTCTCACGTCACTGATGGAAGAGTTACCATGTGATGAGAGTAATGATGAAAGGTTAGATAGTTTAATAAGATCTTTTGAGGCTGAAATTAGTGAAAGCAAGATGGGTGGCCATGATAATTCAACATGCATAGGATCACAATTGAAGAGCAATTTTGAAGAATATTATGATGAATCATGGAACATAGGACAAGTGATGGAGGAGGGACATGACTTTGGGGTTGAGTGGGTGGATATGGATTTGATGACATCCTTCCAATTTGATGATGCAAGTTGGGAGGCAATTGAGTCTTGTGGAGATGAGAAAGATGTGATGGTTGATCATTTAATGGTTTGTGATGATGGGTTTGATATGGAAGAACATGCTTACAATTCCTTTTGGCAAGATAGGTATGAGATGGGGTTGGTGCATTAG

Protein sequence

>MS.gene64349.t1

MASQVCDTFACDVEIKGDLLTSLMEELPCDESNDERLDSLIRSFEAEISESKMGGHDNSTCIGSQLKSNFEEYYDESWNIGQVMEEGHDFGVEWVDMDLMTSFQFDDASWEAIESCGDEKDVMVDHLMVCDDGFDMEEHAYNSFWQDRYEMGLVH