AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64434

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64434.t1 MTR_2g041640 97.819 871 17 2 1 870 1 870 0.0 1763
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64434.t1 AT1G77550 67.517 862 267 5 10 870 3 852 0.0 1201

Find 215 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCCACTTTCCAATATTT+TGG 0.092480 2.4:+48537531 None:intergenic
CATATTATATCTGCATCTTT+TGG 0.139160 2.4:+48534759 None:intergenic
TATTCCTGTCAGATTGTTTC+TGG 0.155637 2.4:-48534043 MS.gene64434:CDS
CAATTTCGGGGAGTGGGCTT+TGG 0.216553 2.4:-48538273 MS.gene64434:CDS
CAACTTTCACAAGCAATTTC+GGG 0.220240 2.4:-48538286 MS.gene64434:CDS
GGAATTCTACTCGCCGCTTC+AGG 0.227443 2.4:-48540245 MS.gene64434:CDS
CACATGATGGGCGTCCTTTA+CGG 0.233516 2.4:-48536334 MS.gene64434:CDS
TCAACTTTCACAAGCAATTT+CGG 0.237496 2.4:-48538287 MS.gene64434:CDS
ACTCATCTGTCTCAACTTAT+TGG 0.250244 2.4:-48534299 MS.gene64434:CDS
CCAAACATTTCTGAACTAAA+TGG 0.271808 2.4:-48537565 MS.gene64434:CDS
AGTTTCCATGAGTCGGATTA+TGG 0.278974 2.4:+48534172 None:intergenic
TGCACTCGTGACTTTCTTCT+TGG 0.281535 2.4:-48536609 MS.gene64434:CDS
ATTCTCTTGCTTACGTTCAT+TGG 0.283449 2.4:-48537768 MS.gene64434:CDS
GGGAAGTGCCATTGACATTC+TGG 0.284815 2.4:-48537596 MS.gene64434:CDS
CTGTATCTCTTGCAGTTAAA+TGG 0.290895 2.4:-48533595 MS.gene64434:intron
TTAGTGGCACTTAGCCTATA+TGG 0.294517 2.4:-48538561 MS.gene64434:CDS
AGGTACGTGATGGATGAATT+TGG 0.296880 2.4:-48537076 MS.gene64434:CDS
GTCGATATTCCTGGTCCTCT+TGG 0.300317 2.4:-48537619 MS.gene64434:CDS
TCAGCTTGATCGGCATTAGA+TGG 0.304912 2.4:+48538218 None:intergenic
CAGGTTGTTCAATATACTTC+TGG 0.310081 2.4:+48534135 None:intergenic
TATTTGTGCATCCAAAATAT+TGG 0.310689 2.4:-48537542 MS.gene64434:CDS
ACTTCTTGAATAATCAATTC+AGG 0.312500 2.4:+48538522 None:intergenic
CAAAAGATGCAGATATAATA+TGG 0.314062 2.4:-48534758 MS.gene64434:CDS
AGCTAACAACCAATATTCTT+TGG 0.314596 2.4:-48533926 MS.gene64434:CDS
CGTGACTTTCTTCTTGGTAT+TGG 0.317812 2.4:-48536603 MS.gene64434:CDS
TGAAACTCATTTCACTGTTA+TGG 0.319368 2.4:-48533881 MS.gene64434:intron
AATAGTGCGGGCCATGTTCC+AGG 0.320213 2.4:+48534223 None:intergenic
GTTGATGATGATGATGTTTC+GGG 0.325483 2.4:-48539384 MS.gene64434:CDS
AATTTCGGGGAGTGGGCTTT+GGG 0.325758 2.4:-48538272 MS.gene64434:CDS
ACTTACCAATTCTGAAGAAC+TGG 0.326545 2.4:+48538456 None:intergenic
TGATGATATGTTTCTCTCAT+TGG 0.333223 2.4:-48539256 MS.gene64434:CDS
ACTTGGATAAAATAGTTCTC+TGG 0.333720 2.4:+48536534 None:intergenic
AATAAATCACAAGAATATTA+AGG 0.336524 2.4:-48533746 MS.gene64434:CDS
TCGATATTCCTGGTCCTCTT+GGG 0.347686 2.4:-48537618 MS.gene64434:CDS
TATGGCCAACACGGAATGTT+TGG 0.355349 2.4:-48536644 MS.gene64434:CDS
TCTTAAGGAGTTACCAGAAC+TGG 0.362100 2.4:-48538321 MS.gene64434:CDS
ACATGATGGGCGTCCTTTAC+GGG 0.362567 2.4:-48536333 MS.gene64434:CDS
ATAACCCAGTTCTTCAGAAT+TGG 0.365410 2.4:-48538461 MS.gene64434:intron
AAGTTATACATCTAATTTCC+AGG 0.366200 2.4:+48533004 None:intergenic
TCTAGTGATGGTGATCTTGC+AGG 0.366268 2.4:-48538350 MS.gene64434:intron
AGACGGCGGTGGAGGGATTC+CGG 0.366662 2.4:+48540218 None:intergenic
TTGTAAGCTTGTTTCCATAT+AGG 0.366689 2.4:+48538547 None:intergenic
GGCTCATCAGGAGTCCATTC+AGG 0.370563 2.4:+48537475 None:intergenic
TCTTGCAGGTATTATTCTTA+AGG 0.373169 2.4:-48538336 MS.gene64434:CDS
GTTGAAGAAATCACAACCTT+TGG 0.377369 2.4:+48533156 None:intergenic
ACTCGCCGCTTCAGGGTTAC+CGG 0.378225 2.4:-48540237 MS.gene64434:CDS
CTCGCCGCCGTCGAAGGTTT+CGG 0.378738 2.4:+48540178 None:intergenic
CTTTCCCTTTCAGAATTTAG+TGG 0.379886 2.4:-48538577 MS.gene64434:intron
AAAGGTGCCACTCTAAAGTT+TGG 0.383885 2.4:+48537028 None:intergenic
TGAACAACCTGCTTTATTCC+AGG 0.384791 2.4:-48534123 MS.gene64434:CDS
GATGTGTGTGGATATTGATT+TGG 0.393550 2.4:-48539430 MS.gene64434:CDS
TTGCAAGCTCGTGTACAATC+AGG 0.394641 2.4:+48533217 None:intergenic
GAAGACTTCGCCAAAGTTCA+CGG 0.397878 2.4:-48540266 MS.gene64434:CDS
GGACTTGCCTGCTAGATTCC+CGG 0.398457 2.4:-48539235 MS.gene64434:intron
TGTTGATGATGATGATGTTT+CGG 0.400062 2.4:-48539385 MS.gene64434:CDS
TTCTTCCTCGCCTTCCTGAA+TGG 0.401426 2.4:-48537489 MS.gene64434:CDS
CATTAATCAATTTCCATTTG+AGG 0.403628 2.4:-48534676 MS.gene64434:CDS
GAATTATAAATCTCCAGTTC+TGG 0.404024 2.4:+48538308 None:intergenic
GAGAAGGACTAGACAACCTT+TGG 0.406467 2.4:-48534256 MS.gene64434:CDS
CTTTCGTACTTGAACATTCG+TGG 0.406787 2.4:-48537842 MS.gene64434:CDS
CGGTCATGAATAGGCTCATC+AGG 0.408012 2.4:+48537463 None:intergenic
CCAACATCAAGGGCAATTTA+CGG 0.409401 2.4:-48533491 MS.gene64434:CDS
CCATTTAGTTCAGAAATGTT+TGG 0.413238 2.4:+48537565 None:intergenic
GGTTCGGAGCATGCACCCTT+TGG 0.416642 2.4:-48534069 MS.gene64434:CDS
GCCACATTGCACTTATAATA+CGG 0.416942 2.4:+48537443 None:intergenic
CTCTCAGAGGACTGCACAGT+GGG 0.420225 2.4:+48536371 None:intergenic
GGTTAGTGTTGTAGAAATGT+TGG 0.423832 2.4:-48539355 MS.gene64434:CDS
ATAATCCGACTCATGGAAAC+TGG 0.425940 2.4:-48534170 MS.gene64434:CDS
CTTATAATACGGTCATGAAT+AGG 0.427782 2.4:+48537454 None:intergenic
GAATTCTACTCGCCGCTTCA+GGG 0.429428 2.4:-48540244 MS.gene64434:CDS
ATGAATTTGGTTCTGCTTTG+CGG 0.431191 2.4:-48537063 MS.gene64434:CDS
CTCTTGCTTACGTTCATTGG+AGG 0.433275 2.4:-48537765 MS.gene64434:intron
TTGAGATGGTTGGAGCTTGA+TGG 0.434714 2.4:-48539291 MS.gene64434:CDS
CGAAAGGGATGGCAAACAAA+TGG 0.435231 2.4:+48537859 None:intergenic
CTTGGATAAAATAGTTCTCT+GGG 0.435795 2.4:+48536535 None:intergenic
TCTTCCTCGTTGATCATGCT+TGG 0.437053 2.4:-48540073 MS.gene64434:CDS
GTAACTGAGCTTTGCTCCAA+TGG 0.447485 2.4:+48537815 None:intergenic
CACAAGCAATTTCGGGGAGT+GGG 0.449915 2.4:-48538279 MS.gene64434:CDS
GCTGCGTGAAGTTCCGGGAT+TGG 0.452297 2.4:-48539472 MS.gene64434:CDS
TATGACATGGATATCGTTGT+TGG 0.452356 2.4:-48533193 MS.gene64434:CDS
TCTCTTCGCAAGGTTCGATC+TGG 0.454357 2.4:+48540150 None:intergenic
TGATCTCCGTTATGTAGTAC+TGG 0.455881 2.4:-48534090 MS.gene64434:CDS
AGGTTTCGGTGGAGAGTTTG+TGG 0.460242 2.4:+48540192 None:intergenic
GATGTTGGACTATGCATCTC+AGG 0.462658 2.4:+48533506 None:intergenic
CAGTGGACTGTCAGCTTGAT+CGG 0.463965 2.4:+48538208 None:intergenic
TTTAGCAGAAACAATTCAAA+AGG 0.464036 2.4:-48534631 MS.gene64434:intron
TTACCAACCTCTAGTAACTT+AGG 0.465893 2.4:+48533437 None:intergenic
ATGACAAGACAAGCCTCAAA+TGG 0.465912 2.4:+48534663 None:intergenic
ACTCTCCGACGCTTACAAAC+AGG 0.466139 2.4:-48540042 MS.gene64434:intron
ATTCCTGTCAGATTGTTTCT+GGG 0.469381 2.4:-48534042 MS.gene64434:intron
CCCTCTGGCATGAAAAGAAA+AGG 0.470377 2.4:+48537010 None:intergenic
TCCGTTATGTAGTACTGGTT+CGG 0.471117 2.4:-48534085 MS.gene64434:CDS
GTTCGAGAGGTGAAAGAGAA+AGG 0.471606 2.4:-48539324 MS.gene64434:CDS
TTTATCTTGCTTCAGGCACA+TGG 0.472517 2.4:-48534359 MS.gene64434:intron
TGTGGAAGAGACGGCGGTGG+AGG 0.472903 2.4:+48540210 None:intergenic
CACAGTGGGTGGTATTAAAC+TGG 0.473738 2.4:+48536385 None:intergenic
TCCGGGATTGGCAGAGAGGA+TGG 0.477944 2.4:-48539460 MS.gene64434:CDS
ATTGAACCAAGCAGTAAGTC+TGG 0.478752 2.4:+48536560 None:intergenic
ATCTCTTGCAGTTAAATGGT+TGG 0.478877 2.4:-48533591 MS.gene64434:intron
TCACAAGCAATTTCGGGGAG+TGG 0.481347 2.4:-48538280 MS.gene64434:CDS
CCCATCCTCTCTGCCAATCC+CGG 0.482424 2.4:+48539459 None:intergenic
TGGATATACATGCAAGAGTA+AGG 0.489710 2.4:-48533571 MS.gene64434:CDS
TATAATATGGACCTGCGTAC+AGG 0.490503 2.4:-48534745 MS.gene64434:CDS
CAACCTCTAGTAACTTAGGT+TGG 0.493695 2.4:+48533441 None:intergenic
CCGTAAATTGCCCTTGATGT+TGG 0.495083 2.4:+48533491 None:intergenic
TATGATTCGATCGGTGTTTG+AGG 0.499225 2.4:-48533546 MS.gene64434:CDS
CGATATTCCTGGTCCTCTTG+GGG 0.503309 2.4:-48537617 MS.gene64434:CDS
GAAATGCTCGCCGCCGTCGA+AGG 0.504521 2.4:+48540172 None:intergenic
GAACATTCGTGGAAATCCAT+TGG 0.506538 2.4:-48537831 MS.gene64434:CDS
GAGTAGAATTCCGTGAACTT+TGG 0.507996 2.4:+48540256 None:intergenic
AGAAGTATTCGCCCACATGA+TGG 0.518087 2.4:-48536347 MS.gene64434:CDS
AGGCACATGGATCTCCTCAA+TGG 0.518425 2.4:-48534346 MS.gene64434:CDS
TTGGACCAGTTTCCATGAGT+CGG 0.519542 2.4:+48534165 None:intergenic
TGATGATGTTTCGGGAGAGA+AGG 0.520808 2.4:-48539376 MS.gene64434:CDS
ATCAAATTTAAGCATCTCAA+AGG 0.523069 2.4:-48538498 MS.gene64434:CDS
CTCATGCGATTCTCTTCGCA+AGG 0.523435 2.4:+48540140 None:intergenic
GAAAGGGATGGCAAACAAAT+GGG 0.527047 2.4:+48537860 None:intergenic
TGAGTTTCAAGATTATAAGT+AGG 0.529055 2.4:+48534317 None:intergenic
CAGCTTGATCGGCATTAGAT+GGG 0.529614 2.4:+48538219 None:intergenic
CCTTTCCAAACATTCCGTGT+TGG 0.533360 2.4:+48536639 None:intergenic
TACAGTAGTATCAATAGTGC+GGG 0.533708 2.4:+48534211 None:intergenic
GCCGATGCTAGATTGCCCTC+TGG 0.535309 2.4:+48536995 None:intergenic
TTAAGCATCTCAAAGGACTC+TGG 0.537705 2.4:-48538491 MS.gene64434:CDS
ACCTTTGGATCTTAAAACCC+TGG 0.538572 2.4:-48534241 MS.gene64434:CDS
GAAGTATTCGCCCACATGAT+GGG 0.539104 2.4:-48536346 MS.gene64434:CDS
TGTAGAAATGTTGGAGAGTG+AGG 0.539327 2.4:-48539346 MS.gene64434:CDS
AGTTAGACGAAACCTCTGTA+TGG 0.539543 2.4:-48537384 MS.gene64434:intron
CACGAATGTTCAAGTACGAA+AGG 0.541790 2.4:+48537843 None:intergenic
GAACAACCTGCTTTATTCCA+GGG 0.542789 2.4:-48534122 MS.gene64434:CDS
TTACTGACCGGGAATCTAGC+AGG 0.542860 2.4:+48539228 None:intergenic
ATGGATATCGTTGTTGGAGA+AGG 0.543040 2.4:-48533187 MS.gene64434:CDS
GGATAATTCTAGGTACGTGA+TGG 0.545152 2.4:-48537086 MS.gene64434:intron
AGAAGAAGAAATGGTAGCAA+TGG 0.546649 2.4:-48540318 None:intergenic
TGCAAGCTCGTGTACAATCA+GGG 0.549178 2.4:+48533218 None:intergenic
GCATCCAAAATATTGGAAAG+TGG 0.551382 2.4:-48537535 MS.gene64434:CDS
GTTTGTGGAAGAGACGGCGG+TGG 0.553090 2.4:+48540207 None:intergenic
CGTAAAGGACGCCCATCATG+TGG 0.553316 2.4:+48536335 None:intergenic
GTTGGAGAGTGAGGTTCGAG+AGG 0.553772 2.4:-48539337 MS.gene64434:CDS
AACAGCCATAATCCGACTCA+TGG 0.553916 2.4:-48534177 MS.gene64434:CDS
ATATCTGTGTATACCCGTAA+AGG 0.555996 2.4:+48536320 None:intergenic
AACTTTGGCGAAGTCTTCGA+AGG 0.557163 2.4:+48540271 None:intergenic
CCGGGATTGGCAGAGAGGAT+GGG 0.560887 2.4:-48539459 MS.gene64434:CDS
GTTCTGCCAGACTTACTGCT+TGG 0.561878 2.4:-48536566 MS.gene64434:CDS
AAGAGATTCCAGAATGTCAA+TGG 0.562794 2.4:+48537588 None:intergenic
GATCTTAAAACCCTGGAACA+TGG 0.564811 2.4:-48534234 MS.gene64434:CDS
CTTACCAATTCTGAAGAACT+GGG 0.565554 2.4:+48538457 None:intergenic
ATCGGTGTTTGAGGCAGCTG+CGG 0.566183 2.4:-48533537 MS.gene64434:CDS
TGACGGTACATTGAGATGGT+TGG 0.567506 2.4:-48539301 MS.gene64434:CDS
TCCACCGAAACCTTCGACGG+CGG 0.568274 2.4:-48540182 MS.gene64434:CDS
TTGGACCTGTTTGTAAGCGT+CGG 0.569827 2.4:+48540037 None:intergenic
TTGCTGATCTGTTATTCCTG+TGG 0.573730 2.4:+48534701 None:intergenic
GAGGAGTTGAAGAAGGCCAC+AGG 0.580194 2.4:-48534717 MS.gene64434:CDS
ATACCCAGAAACAATCTGAC+AGG 0.581860 2.4:+48534039 None:intergenic
AGATGCATAGTCCAACATCA+AGG 0.583143 2.4:-48533502 MS.gene64434:CDS
ACGAATGTTCAAGTACGAAA+GGG 0.584367 2.4:+48537844 None:intergenic
GTGGAAGAGACGGCGGTGGA+GGG 0.585364 2.4:+48540211 None:intergenic
GGTTGATGAGGAGTTGAAGA+AGG 0.587491 2.4:-48534724 MS.gene64434:CDS
AACAACCTGCTTTATTCCAG+GGG 0.587507 2.4:-48534121 MS.gene64434:CDS
TTTCCAACCTAAGTTACTAG+AGG 0.588700 2.4:-48533444 MS.gene64434:intron
TTGCACAGAGATATATGGAA+AGG 0.594711 2.4:-48538246 MS.gene64434:CDS
CCGACTTGTTAGAGCTGCTG+AGG 0.595289 2.4:-48537793 MS.gene64434:CDS
GCCGCCGTCGAAGGTTTCGG+TGG 0.597756 2.4:+48540181 None:intergenic
GCCAGAGGGCAATCTAGCAT+CGG 0.598031 2.4:-48536996 MS.gene64434:CDS
GGAGAAGGAGGTATTGCCAA+AGG 0.601959 2.4:-48533172 MS.gene64434:CDS
CACTTACTGATTGCAAACTC+AGG 0.606354 2.4:+48536269 None:intergenic
AACTTTCACAAGCAATTTCG+GGG 0.606957 2.4:-48538285 MS.gene64434:CDS
TTTCTATTCTATGGCCAACA+CGG 0.607111 2.4:-48536653 MS.gene64434:CDS
ATAGTGCGGGCCATGTTCCA+GGG 0.608612 2.4:+48534224 None:intergenic
CTGACAGGAATATCTCCAAA+GGG 0.611128 2.4:+48534054 None:intergenic
TCTGACAGGAATATCTCCAA+AGG 0.611646 2.4:+48534053 None:intergenic
GGAGTCCATTCAGGAAGGCG+AGG 0.613302 2.4:+48537484 None:intergenic
TGGAGAGTTTGTGGAAGAGA+CGG 0.613450 2.4:+48540201 None:intergenic
GATAGTGAAACTGAGGTGAA+TGG 0.613747 2.4:-48539408 MS.gene64434:CDS
ATGATCAACGAGGAAGACAT+TGG 0.615083 2.4:+48540079 None:intergenic
GATATCGTTGTTGGAGAAGG+AGG 0.622037 2.4:-48533184 MS.gene64434:CDS
AGTAAGGACTATGATTCGAT+CGG 0.623174 2.4:-48533555 MS.gene64434:CDS
GCTCTCAGAGGACTGCACAG+TGG 0.626388 2.4:+48536370 None:intergenic
CATTCACAATCTGATCAACA+AGG 0.628020 2.4:-48538150 MS.gene64434:intron
GATGGGTTCTTTGATGTGTG+TGG 0.629001 2.4:-48539442 MS.gene64434:CDS
GATGCATAGTCCAACATCAA+GGG 0.630418 2.4:-48533501 MS.gene64434:CDS
TATTATGTACGCAAAAGAGA+AGG 0.632002 2.4:-48534272 MS.gene64434:CDS
CTCCTCATCAACCTGTACGC+AGG 0.634177 2.4:+48534734 None:intergenic
TCCGAACCAGTACTACATAA+CGG 0.635574 2.4:+48534084 None:intergenic
CCAACACGGAATGTTTGGAA+AGG 0.635916 2.4:-48536639 MS.gene64434:CDS
ATGTTCAAGTACGAAAGGGA+TGG 0.635997 2.4:+48537848 None:intergenic
GCATGTTCTCAGAATTATCG+TGG 0.636330 2.4:-48533772 MS.gene64434:intron
TCAGAGGACTGCACAGTGGG+TGG 0.639769 2.4:+48536374 None:intergenic
AAGGTGACGGTACATTGAGA+TGG 0.641533 2.4:-48539305 MS.gene64434:CDS
GTCAAGTACTCTTCCACGTG+AGG 0.644264 2.4:+48536296 None:intergenic
AAGTTCCGGGATTGGCAGAG+AGG 0.644963 2.4:-48539464 MS.gene64434:CDS
AGTGGGTGGTATTAAACTGG+TGG 0.645039 2.4:+48536388 None:intergenic
GACCTGCGTACAGGTTGATG+AGG 0.645215 2.4:-48534736 MS.gene64434:CDS
GGATTCCGGTAACCCTGAAG+CGG 0.645492 2.4:+48540232 None:intergenic
AGATTAGATACACTCAGCAG+TGG 0.646587 2.4:+48538191 None:intergenic
TTACAGTAGTATCAATAGTG+CGG 0.648290 2.4:+48534210 None:intergenic
GCAATCTAGCATCGGCTGTG+AGG 0.649411 2.4:-48536988 MS.gene64434:intron
GAATTTGAGGAAGAACATCA+AGG 0.650482 2.4:-48533712 MS.gene64434:intron
ACGAGCTTGCAAATATGACA+TGG 0.651494 2.4:-48533206 MS.gene64434:CDS
CCTCAGCAGCTCTAACAAGT+CGG 0.652420 2.4:+48537793 None:intergenic
CATCAGGAGTCCATTCAGGA+AGG 0.654679 2.4:+48537479 None:intergenic
CTCTCCACCGAAACCTTCGA+CGG 0.658578 2.4:-48540185 MS.gene64434:CDS
GATATTCCTGGTCCTCTTGG+GGG 0.661353 2.4:-48537616 MS.gene64434:CDS
GAGGTGAAAGAGAAAGGTGA+CGG 0.664017 2.4:-48539318 MS.gene64434:CDS
TGATTTGGATAGTGAAACTG+AGG 0.668818 2.4:-48539415 MS.gene64434:CDS
TAAGTAGGCTGCAACCATTG+AGG 0.676253 2.4:+48534332 None:intergenic
TGATGAGCCAAACTTTAGAG+TGG 0.680214 2.4:-48537035 MS.gene64434:CDS
ATACACAGATATACCTCACG+TGG 0.685643 2.4:-48536309 MS.gene64434:CDS
GTAAAGGACGCCCATCATGT+GGG 0.688444 2.4:+48536336 None:intergenic
GCGAATACTTCTGCTCTCAG+AGG 0.689727 2.4:+48536358 None:intergenic
ATGAATGTCATACCATACAG+AGG 0.704504 2.4:+48537372 None:intergenic
TTGGATAAAATAGTTCTCTG+GGG 0.706460 2.4:+48536536 None:intergenic
AGAGTTTGTGGAAGAGACGG+CGG 0.716886 2.4:+48540204 None:intergenic
TAGAATCCGAAGTCAAAACG+AGG 0.732532 2.4:+48540114 None:intergenic
ACCGTATTATAAGTGCAATG+TGG 0.733685 2.4:-48537444 MS.gene64434:CDS
AAGTCCAAGCATGATCAACG+AGG 0.739980 2.4:+48540069 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.4 gene 48533015 48540330 48533015 ID=MS.gene64434
chr2.4 mRNA 48533015 48540330 48533015 ID=MS.gene64434.t1;Parent=MS.gene64434
chr2.4 exon 48540043 48540330 48540043 ID=MS.gene64434.t1.exon1;Parent=MS.gene64434.t1
chr2.4 CDS 48540043 48540330 48540043 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48539236 48539493 48539236 ID=MS.gene64434.t1.exon2;Parent=MS.gene64434.t1
chr2.4 CDS 48539236 48539493 48539236 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48538462 48538586 48538462 ID=MS.gene64434.t1.exon3;Parent=MS.gene64434.t1
chr2.4 CDS 48538462 48538586 48538462 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48538151 48538367 48538151 ID=MS.gene64434.t1.exon4;Parent=MS.gene64434.t1
chr2.4 CDS 48538151 48538367 48538151 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48537766 48537891 48537766 ID=MS.gene64434.t1.exon5;Parent=MS.gene64434.t1
chr2.4 CDS 48537766 48537891 48537766 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48537385 48537641 48537385 ID=MS.gene64434.t1.exon6;Parent=MS.gene64434.t1
chr2.4 CDS 48537385 48537641 48537385 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48536989 48537096 48536989 ID=MS.gene64434.t1.exon7;Parent=MS.gene64434.t1
chr2.4 CDS 48536989 48537096 48536989 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48536536 48536677 48536536 ID=MS.gene64434.t1.exon8;Parent=MS.gene64434.t1
chr2.4 CDS 48536536 48536677 48536536 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48536276 48536441 48536276 ID=MS.gene64434.t1.exon9;Parent=MS.gene64434.t1
chr2.4 CDS 48536276 48536441 48536276 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48534632 48534789 48534632 ID=MS.gene64434.t1.exon10;Parent=MS.gene64434.t1
chr2.4 CDS 48534632 48534789 48534632 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48534043 48534366 48534043 ID=MS.gene64434.t1.exon11;Parent=MS.gene64434.t1
chr2.4 CDS 48534043 48534366 48534043 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48533882 48533956 48533882 ID=MS.gene64434.t1.exon12;Parent=MS.gene64434.t1
chr2.4 CDS 48533882 48533956 48533882 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48533713 48533782 48533713 ID=MS.gene64434.t1.exon13;Parent=MS.gene64434.t1
chr2.4 CDS 48533713 48533782 48533713 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48533445 48533602 48533445 ID=MS.gene64434.t1.exon14;Parent=MS.gene64434.t1
chr2.4 CDS 48533445 48533602 48533445 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48533117 48533251 48533117 ID=MS.gene64434.t1.exon15;Parent=MS.gene64434.t1
chr2.4 CDS 48533117 48533251 48533117 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
chr2.4 exon 48533015 48533041 48533015 ID=MS.gene64434.t1.exon16;Parent=MS.gene64434.t1
chr2.4 CDS 48533015 48533041 48533015 ID=cds.MS.gene64434.t1;Parent=MS.gene64434.t1
Gene Sequence

>MS.gene64434

ATGGTAGCAATGGCAACAACAACAAAGAGAATCGAAACCTTCGAAGACTTCGCCAAAGTTCACGGAATTCTACTCGCCGCTTCAGGGTTACCGGAATCCCTCCACCGCCGTCTCTTCCACAAACTCTCCACCGAAACCTTCGACGGCGGCGAGCATTTCCAGATCGAACCTTGCGAAGAGAATCGCATGAGACGCCTCGTTTTGACTTCGGATTCTATGGCTAAAGACTCCAATGTCTTCCTCGTTGATCATGCTTGGACTTTCAGACTCTCCGACGCTTACAAACAGGTCCAAACCTTCTTCACATTCTTCATGCAACACTAGTTTTACATAACTAATGAAAATAATTTGTAGCTTATACGCAAATAACATTATTTTATCTTTGTACATAAGTGTTTATCATGATAATAATTAATAAGCACTTATGCTATAAGTTGCAAATTAAGTTGTTTATCCAAACATAGCTTAAATTAATTGTAGGTGGTTAAATATGAGGACCAAATTGATTGATAGTTAGTTGTTAAACTATAGGGAGCTAATAGATAGTAAGTGGTTAGTTATATTATAGGACTAACTTGACAGTTTAATAGAGTAAGTGATATTTTTGAGATATTCATAATCTCAAAGTGTCTTAGAATGTTCGGGACTAATTTGGTTATTTACTCACTAATCTTTTATTTCTAGGGTTTAATTTTTACTCACTAACATTATGGCGTAATTTTATCCATGTAGCCGACCCAACTTACTGGGATAAGGCTTAGTTGTAGTAGTAGGTAGGGTTTCATTTTTGCGTTAATTTTGTTGATTTTTTGTTGTTGTTCTTTTTGGCGATTTTAGCTGCGTGAAGTTCCGGGATTGGCAGAGAGGATGGGTTCTTTGATGTGTGTGGATATTGATTTGGATAGTGAAACTGAGGTGAATGGTGTTGATGATGATGATGTTTCGGGAGAGAAGGTTAGTGTTGTAGAAATGTTGGAGAGTGAGGTTCGAGAGGTGAAAGAGAAAGGTGACGGTACATTGAGATGGTTGGAGCTTGATGGTCTTGATATCGATGATGATATGTTTCTCTCATTGGACTTGCCTGCTAGATTCCCGGTCAGTAACTCTTAATCTTGTTTTTTTTATTTTGTTCATATTTTATTTTATAGTTATAATTTCTATAGGTTCTATTGTTTTGAAAGTGTGACTTTGTTCATTCTGCAAATAGAAATCATGCTCATCAAACTTAGGAATGTTATATTATGATATGAATTGAATTTGAGTGGCAAGATGGCAAACATTATTACTTCCTTCATCCGTTATTATAAGACTCACTTTTGACTTTTGTTAAAAAAATTAAGACTTTTGATATTTCCTTGTTCTTTAATATCGGACTCTTTTCAAAATTTTACATTCACTAATAATTACTTTACAAAAATTTCCTTATTTAATTTACAATTGTGAAACGTAAATTTGTTTTTTCACTCTTACAACATCAATAATTGTGAGGATATTTATAGGAAGTAATACAAATTTGACAGGTTTATTGTTATGGCTATTTTTCTTAATACTGAGTAAATTTTGTAAGCGAGTCTCATAATAAGAGACGATGACAGTATTATTTAATTATCGTCGGAGTATTCAATTTTCCTGTCATCTCAGATCCCGTTATTCTAGATGCTGCAAGCCAATTTTAGTTTCACGTTTCTTGTTATAATTATTTTAATTTGAATTCTGATGAACTATCCTCCCCTTTCCCTTTCAGAATTTAGTGGCACTTAGCCTATATGGAAACAAGCTTACAAGACCTGAATTGATTATTCAAGAAGTTATCAAATTTAAGCATCTCAAAGGACTCTGGCTGAACAATAACCCAGTTCTTCAGAATTGGTAAGTTTTCTCATATTTTAAAAATTTCTTAAACCACATATTCCTTTTTGGTAAGTCCTTGTTGTTAAGGAGAGCTAAATTACTTTACTTCTAGTGATGGTGATCTTGCAGGTATTATTCTTAAGGAGTTACCAGAACTGGAGATTTATAATTCAACTTTCACAAGCAATTTCGGGGAGTGGGCTTTGGGCTTTTGCACAGAGATATATGGAAAGGATAACCCATCTAATGCCGATCAAGCTGACAGTCCACTGCTGAGTGTATCTAATCTTGATCTTTCAAATAGAAACATTCACAATCTGATCAACAAGGTTGAACCTTTTGTTATGCTTATTTTCTATCTTATTGGCTTATGTTTCAAAAGTATAGATAGACTTAAGCATATTATTGTACTGAGTTCAAAAATCTGATAATTATGAATCTCTCCTTGAGTCTATGCTCAAGAGATACAAAAAACAAAGCATAATCACTAAAAAGATTTTAAACACATGGCATTGCCAATTCATTTTTTCCAATCCCATATATGTATGTTTTGATCGAATTATTTTGACGATTCCCTCATTTGGTTAGGACTTTTCACCCATTTGTTTGCCATCCCTTTCGTACTTGAACATTCGTGGAAATCCATTGGAGCAAAGCTCAGTTACCGACTTGTTAGAGCTGCTGAGGCGATTCTCTTGCTTACGTTCATTGGAGGTACTTTGATTTATGTTATGACAATTTTTCAAGCCCCAAATTGCCTTTGTTATTTATTTAATTTTGTATGTCCATTCTCTGGTAACCAGTGTTTTGGCCTTTGGGAATTTGAGCAAATTTTTAGGTCGATATTCCTGGTCCTCTTGGGGGAAGTGCCATTGACATTCTGGAATCTCTTCCAAACATTTCTGAACTAAATGGTATTTGTGCATCCAAAATATTGGAAAGTGGAAAGCATGTTATTGATTCAGTGCTTCTTCCTCGCCTTCCTGAATGGACTCCTGATGAGCCTATTCATGACCGTATTATAAGTGCAATGTGGCAATATCTAATGACATATAGACTTGCTGATGAAGAAAAGTTAGACGAAACCTCTGTATGGTATGACATTCATTTTACTTGTTTTACTTTCTTGGTTCTAATGTAGCACTAGGTTTCTATCAATCAGTAAATGCTTTTAAGTTTTGTGTTTTTGCACTTCTTGTTGAAATACACAATTTCATCAAGGTTGTCTGATTTGTAAATTATTGTTGTGACCCTACTCGAATCAATGGGTCAATGTTTAACCATTCAAATTGTTGGGTCAGTACATTATATGAAACATATGATCAATTTGGAATTTCCCAACAATAAAGAAAACTAACATCCCATTGTTTTGGATAATTCTAGGTACGTGATGGATGAATTTGGTTCTGCTTTGCGGCACAGTGATGAGCCAAACTTTAGAGTGGCACCTTTTCTTTTCATGCCAGAGGGCAATCTAGCATCGGCTGTGAGGTTTTCTTCTATATTTTACTTTTATATTTTGCTTTTCTACTATATTTTACTTTCTGCTTTACTATATGATTGAGCTTGCATGATTTGTAAAAAAATATTATATAAAAAAATTCGATTATAGGTTTTGCTCGTTAATTCTTGCTCATTAATATATAATAACTTAATTACCTGTGTAGCTCATATTAATGACTTCTAACTGCGAAACTTCTCCTCCATCCTTTTTATGAATTACATGTATTTTATGGCATGCCCTCTGGATTTGTGATAATTCTAAATTAGTCTCTGTAAGTAATAACTCCCACTTTTCACAGCTTTTCTATTCTATGGCCAACACGGAATGTTTGGAAAGGCGATGAATGCACTCGTGACTTTCTTCTTGGTATTGGAGAAGATAAACAACGTTCTGCCAGACTTACTGCTTGGTTCAATACCCCAGAGAACTATTTTATCCAAGTAATTCTTCTATTACTAAGGCAGATTATGATTCATATCTGTACCACACGAAAGTTTTAGGGAAGTTTGAGGCTAATTATTTTATTATTTTCAGGAGTATGAGAAGTACAATCAGAGATTGCAATCCACCAGTTTAATACCACCCACTGTGCAGTCCTCTGAGAGCAGAAGTATTCGCCCACATGATGGGCGTCCTTTACGGGTATACACAGATATACCTCACGTGGAAGAGTACTTGACACATCCTGAGTTTGCAATCAGTAAGTGGACTCTATGCATCTGATAGACACTGGATTAAATTATAGAGATTAACAGAATGAAAATAATGAAATTTTATCATATATCCTCCTCCAACTTTTGTTCCTTTTTGTTAGGTTGCTTTGAAAAAGGCACAAGCAGGGAATTATTCGTCCCTGAGTTTATTGATAACCGAAAGGTTTATTGATAACAGAAAGAAAAGGGTTACTTTCCTCACCGGAATAAGCCAATTTCTTAATAAAGTTCAGAATGCCTAATCTAGGGGCCGGAGAAGCTATTTATAGATTCCCCGGAATAAGCCAGTCAATTTGTTAATAAAGTTCAGAATGCCTAATCTAGGGTTACAGCCTATGTGACTGGCAGTTGCCAATTAAGACGGTTACAGCCTGTCAAGGCAGTTACAAGGGAAATGCTCTAGTGAGTCACAGCTTATGGTGATCATTCTGAATTCTGGTCACCTACTACCTATATTTGGAGTCAATCCTTTGATATGTCTCACACTTTTCTTGATCTTAAAATTTGTTTTCTTGAACATCACCTCACCTCTCAGAAGCTTTCCCAGGTTTCCTTAGGATTGTTAACCTCCCACTACTTGTACCAATGTTGGAATAGTAGTGCTAGCAACACTCTCTTTTGAGCACTCTCTCTAATACTCACTCTCTTATTGGTTTAAATCATCGTGGGTCTATACTTTGGAAATATAATGGCGAGACATACATTGATTCCATCCAATAAAAGAGTGAGTGCTAGAGAGAGTGTTCAAAAGTGAGTGTTGCTAGCATTCCTCATGTCGGAATGTTCACCCAGTGCTTCCACGCCCATCATTTTCTGTGTCGAAGCGCTCCTCTGCAAATCTTCTTGAATTTACTTCTTGATCAGTCTACACAAAGTTCCCCATTTCTTGGGAGCTTCAGTACTAGTTGATAGGTACTGGTTCAATATATGGAGGCATACAAATGGAAAATGAGGAAGTTTTATAATAATTCCTCCTAACTTAACTCTGGATGAATGGTTATCCAGGTCAAGATGAGCAGGATGACATATAATGATGTCTTCGCCAGGTATTTGAGCTGGTCTCTCCTAAGTCCCACTCAAAACTAATTAACTTCCTCTAGCTTCTTTGCCTCTATTAAACAGCCAAACTAATTAACCACTCTAACAACTTTCCTAACAGAGACTGTGCTTGGCTAGCAGGGTTCCTTGACCTAACTGATGTAAGCTTTCCTAATTGGGTCTGCTCCTAACAACATGCTTGAACTCTTTTATCAACTCATAATATATAGTTAAACAATCATGTAACTTGAAAAGTGAAGAGCCTAAATTGAATTCTCCTTGTTTCATCTGAACCTTTTCTCATTCTGAGAAGCATTGACATCTTATGTTTTTCATAAAATTCCCATGGATATTTGATTCAATGATTACCTCCACGTTTTTAAAGTTTGCTGGATTCTGACCTTCTCCCTCTCTTTTGAATGTGTTTGGCTACAGTAAAGGACCCAAAAGATGCAGATATAATATGGACCTGCGTACAGGTTGATGAGGAGTTGAAGAAGGCCACAGGAATAACAGATCAGCAATACATTAATCAATTTCCATTTGAGGCTTGTCTTGTCATGAAGCATCATTTAGCAGAAACAATTCAAAAGGTGATATTATAGTTAATCAACATTGTATGTTGATGCTGATGAGATTATCCCAAATGTCTGACCTGTTTTCATTATGCATTTCTAGTGAAATTCCATCATACATTCAGAGAAATATGTATTATATTATATATTATATATTCATCAGAGCATGCTACATTTAATTTTATTTTCAAGTCACTTACAAATGAATTGAAGTGTTGGTTTCTCTTTTGAGGCTTTTTAAATAATGAATCATTTTGGGGTTGGGTGTTTTATCTTGCTTCAGGCACATGGATCTCCTCAATGGTTGCAGCCTACTTATAATCTTGAAACTCATCTGTCTCAACTTATTGGTGACTATTATGTACGCAAAAGAGAAGGACTAGACAACCTTTGGATCTTAAAACCCTGGAACATGGCCCGCACTATTGATACTACTGTAACTGATAACTTAACAGCCATAATCCGACTCATGGAAACTGGTCCAAAAATATGCCAGAAGTATATTGAACAACCTGCTTTATTCCAGGGGAAAAAGTTTGATCTCCGTTATGTAGTACTGGTTCGGAGCATGCACCCTTTGGAGATATTCCTGTCAGATTGTTTCTGGGTATACTGATTCTTCTTTCTTTATTTTATTCTTATGCTCTTAAAAGGTTAAACTCTAATGGCAATGAATTTTATGTTTTATTTCAGGTTAGGATAGCTAACAACCAATATTCTTTGGCCAAAAGTAGTCTTTTTGAGTATGAAACTCATTTCACTGTTATGGTATGTCTTGAATTCCATATTGTTTAGCAACAAGATGTTTGGTTGTGCTTCCCTTTTTCTTATCAAGTTTTAACGTATTCAATCAATGCATGTTCTCAGAATTATCGTGGAAGAATAAATCACAAGAATATTAAGGATTTTGTGAGGGAATTTGAGGAAGAACATCAAGGTAATGAGGGCTGCCACATTCTATAGATGAATTTTTAGAGGCTTCCAGAGCATATTATATATTTTGAATGAATTTTTTTATACAAATTCATGTCTCTGTATCTCTTGCAGTTAAATGGTTGGATATACATGCAAGAGTAAGGACTATGATTCGATCGGTGTTTGAGGCAGCTGCGGTTGCACATCCTGAGATGCATAGTCCAACATCAAGGGCAATTTACGGTGTAGATGTCATGTTAGACAGCTCTTTCCAACCTAAGTTACTAGAGGTTGGTAACCGAAACAGTGGTTCTCATTTGATGCTCCTTTCAATACCAAATTTTTTTATATTGACCTGAACATCTTGATTCAAGAATACAAATTTGTAATGTACTATTTGTTTAATGCTGAATTATCTGATTAGGATGCATTTCACCATACGAATTATGGCATTACATGTATGACTTGTATTTTATATTGCAGGTGACTTACTGCCCTGATTGTACACGAGCTTGCAAATATGACATGGATATCGTTGTTGGAGAAGGAGGTATTGCCAAAGGTTGTGATTTCTTCAACAATGTTTTCAAGTGTCTCTTTTTGAATGAAATTTCTCAGGTTAGTCAATTGTAATCTGTTCATCACAGAGATTTGACATCCTTAATTAAAGCCTTCTTCGACAACGGACTACAGGTTCTTTTAGCAAGGCCTGGAAATTAG
Protein sequence

>MS.gene64434.t1

MVAMATTTKRIETFEDFAKVHGILLAASGLPESLHRRLFHKLSTETFDGGEHFQIEPCEENRMRRLVLTSDSMAKDSNVFLVDHAWTFRLSDAYKQLREVPGLAERMGSLMCVDIDLDSETEVNGVDDDDVSGEKVSVVEMLESEVREVKEKGDGTLRWLELDGLDIDDDMFLSLDLPARFPNLVALSLYGNKLTRPELIIQEVIKFKHLKGLWLNNNPVLQNCDGDLAGIILKELPELEIYNSTFTSNFGEWALGFCTEIYGKDNPSNADQADSPLLSVSNLDLSNRNIHNLINKDFSPICLPSLSYLNIRGNPLEQSSVTDLLELLRRFSCLRSLEVDIPGPLGGSAIDILESLPNISELNGICASKILESGKHVIDSVLLPRLPEWTPDEPIHDRIISAMWQYLMTYRLADEEKLDETSVWYVMDEFGSALRHSDEPNFRVAPFLFMPEGNLASAVSFSILWPTRNVWKGDECTRDFLLGIGEDKQRSARLTAWFNTPENYFIQEYEKYNQRLQSTSLIPPTVQSSESRSIRPHDGRPLRVYTDIPHVEEYLTHPEFAIIKDPKDADIIWTCVQVDEELKKATGITDQQYINQFPFEACLVMKHHLAETIQKAHGSPQWLQPTYNLETHLSQLIGDYYVRKREGLDNLWILKPWNMARTIDTTVTDNLTAIIRLMETGPKICQKYIEQPALFQGKKFDLRYVVLVRSMHPLEIFLSDCFWVRIANNQYSLAKSSLFEYETHFTVMNYRGRINHKNIKDFVREFEEEHQVKWLDIHARVRTMIRSVFEAAAVAHPEMHSPTSRAIYGVDVMLDSSFQPKLLEVTYCPDCTRACKYDMDIVVGEGGIAKGCDFFNNVFKCLFLNEISQVLLARPGN