Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64945.t1 | XP_003617669.1 | 78.7 | 277 | 46 | 3 | 1 | 274 | 903 | 1169 | 1.60E-84 | 322.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64945.t1 | G7K4B2 | 78.7 | 277 | 46 | 3 | 1 | 274 | 903 | 1169 | 1.1e-84 | 322.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050442 | MS.gene64945 | 0.81208 | 5.08E-51 | -1.69E-46 |
| MS.gene050750 | MS.gene64945 | 0.804969 | 1.67E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene64945 | -0.825304 | 5.04E-54 | -1.69E-46 |
| MS.gene055358 | MS.gene64945 | 0.807361 | 5.25E-50 | -1.69E-46 |
| MS.gene057026 | MS.gene64945 | 0.802947 | 4.40E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene64945 | 0.815143 | 1.07E-51 | -1.69E-46 |
| MS.gene060553 | MS.gene64945 | 0.80155 | 8.54E-49 | -1.69E-46 |
| MS.gene061114 | MS.gene64945 | -0.809172 | 2.16E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64945.t1 | MTR_5g094150 | 84.116 | 277 | 31 | 3 | 1 | 274 | 903 | 1169 | 6.54e-155 | 461 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 54 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATTATGGATGGTGATTC+TGG | 0.211883 | 5.4:-73854780 | MS.gene64945:CDS |
| CCTGATTGTCGTGCTTTATT+TGG | 0.234766 | 5.4:+73855362 | None:intergenic |
| GCTACTCGTAAATCAAAATT+TGG | 0.248096 | 5.4:-73854510 | MS.gene64945:CDS |
| TTGTGTGCTTCTATGAATAA+AGG | 0.301317 | 5.4:-73855391 | MS.gene64945:CDS |
| AAATTTGCACCCATCCATTC+AGG | 0.309814 | 5.4:-73854471 | MS.gene64945:CDS |
| GTTGGCTGCTGAAAATATTA+TGG | 0.320999 | 5.4:-73854793 | MS.gene64945:CDS |
| CACCCATCCATTCAGGTGGT+TGG | 0.371904 | 5.4:-73854464 | MS.gene64945:CDS |
| CGTACATGTGACCATGCTCC+TGG | 0.380370 | 5.4:+73854823 | None:intergenic |
| CCTGTTTGTCTTGAGTCTCC+TGG | 0.383586 | 5.4:+73854661 | None:intergenic |
| TTCTAACAGGCATGATCGAC+AGG | 0.411514 | 5.4:+73855317 | None:intergenic |
| TTTACCTCACACTTGGCCAT+TGG | 0.425784 | 5.4:+73855436 | None:intergenic |
| AGCTAGATCCTCAATATTGT+TGG | 0.443141 | 5.4:-73855057 | MS.gene64945:CDS |
| GGAGGCAATACACTTCTAAC+AGG | 0.443800 | 5.4:+73855304 | None:intergenic |
| GTTGGGAGATGTTGAGGATA+AGG | 0.445980 | 5.4:-73855012 | MS.gene64945:CDS |
| TGTACATGTGACCATGCTCC+TGG | 0.455027 | 5.4:+73854928 | None:intergenic |
| CTGGATGCTGATTCAGAGAA+AGG | 0.455585 | 5.4:-73855280 | MS.gene64945:CDS |
| CCAAATAAAGCACGACAATC+AGG | 0.457304 | 5.4:-73855362 | MS.gene64945:CDS |
| TAAACAGAAGGCAGTAGTCC+AGG | 0.479284 | 5.4:-73854946 | MS.gene64945:CDS |
| AAATATTACGGAGAGTAAGC+TGG | 0.480063 | 5.4:-73854886 | MS.gene64945:CDS |
| GACTTTAAGAGATCATGATT+CGG | 0.484219 | 5.4:+73854744 | None:intergenic |
| AGGTCGACGATGCAGATAAT+CGG | 0.485330 | 5.4:-73855342 | MS.gene64945:CDS |
| CCAGGAGACTCAAGACAAAC+AGG | 0.485498 | 5.4:-73854661 | MS.gene64945:CDS |
| ATATTATGGATGGTGATTCT+GGG | 0.490562 | 5.4:-73854779 | MS.gene64945:CDS |
| GGCCATTACAATGAGGAAGC+TGG | 0.493545 | 5.4:-73854991 | MS.gene64945:CDS |
| GAGCCAACCACCTGAATGGA+TGG | 0.496619 | 5.4:+73854461 | None:intergenic |
| CGAGTAGCTGCAAAACGAGC+TGG | 0.499101 | 5.4:+73854525 | None:intergenic |
| CTTCGAGCCAACCACCTGAA+TGG | 0.501610 | 5.4:+73854457 | None:intergenic |
| ATCCAGCTTCCTCATTGTAA+TGG | 0.510430 | 5.4:+73854989 | None:intergenic |
| GCACACAATTTCTGACCAGT+TGG | 0.529045 | 5.4:+73855406 | None:intergenic |
| CACACAATTTCTGACCAGTT+GGG | 0.534596 | 5.4:+73855407 | None:intergenic |
| TAGATCCTCAATATTGTTGG+AGG | 0.536002 | 5.4:-73855054 | MS.gene64945:CDS |
| ACATGATGGTAACGCTGACC+AGG | 0.555035 | 5.4:-73854679 | MS.gene64945:CDS |
| TGGATGCTGATTCAGAGAAA+GGG | 0.555256 | 5.4:-73855279 | MS.gene64945:intron |
| TAGAAGTGTATTGCCTCCAC+TGG | 0.561132 | 5.4:-73855299 | MS.gene64945:CDS |
| CACACCCTCCAACAATATTG+AGG | 0.562258 | 5.4:+73855049 | None:intergenic |
| CAGGAGCATGGTCACATGTA+CGG | 0.568783 | 5.4:-73854822 | MS.gene64945:CDS |
| GCACAAGTTGGGAGATGTTG+AGG | 0.575629 | 5.4:-73855018 | MS.gene64945:CDS |
| TTCTCTGAATCAGCATCCAG+TGG | 0.578278 | 5.4:+73855283 | None:intergenic |
| GTACATGTGACCATGCTCCT+GGG | 0.597289 | 5.4:+73854824 | None:intergenic |
| TATTATGGATGGTGATTCTG+GGG | 0.611629 | 5.4:-73854778 | MS.gene64945:CDS |
| AAGGCAGTAGTCCAGGAGCA+TGG | 0.614051 | 5.4:-73854939 | MS.gene64945:CDS |
| AAAGTCATAGCCCAGGAGCA+TGG | 0.615793 | 5.4:-73854834 | MS.gene64945:CDS |
| AGAAAGGCAATTGAAAAGCA+TGG | 0.615795 | 5.4:-73854576 | MS.gene64945:CDS |
| AGCCAACCACCTGAATGGAT+GGG | 0.618326 | 5.4:+73854462 | None:intergenic |
| TTTGCACCCATCCATTCAGG+TGG | 0.621598 | 5.4:-73854468 | MS.gene64945:CDS |
| TGACTTGACTACTAAACAGA+AGG | 0.629581 | 5.4:-73854958 | MS.gene64945:CDS |
| GCTGCTGAAAATATTATGGA+TGG | 0.638442 | 5.4:-73854789 | MS.gene64945:CDS |
| CAAGCCAATGGCCAAGTGTG+AGG | 0.640616 | 5.4:-73855440 | None:intergenic |
| AGATCCTCAATATTGTTGGA+GGG | 0.643952 | 5.4:-73855053 | MS.gene64945:CDS |
| TCTGAATCAGCATCCAGTGG+AGG | 0.655053 | 5.4:+73855286 | None:intergenic |
| TCACATGTACAGTGACAAGT+TGG | 0.674269 | 5.4:-73854916 | MS.gene64945:CDS |
| AGGATAAGGCCATTACAATG+AGG | 0.684721 | 5.4:-73854998 | MS.gene64945:CDS |
| TCACATGTACGGTGACAAGT+TGG | 0.688440 | 5.4:-73854811 | MS.gene64945:CDS |
| TCTAACAGGCATGATCGACA+GGG | 0.691509 | 5.4:+73855318 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGATAAAAATGATAGAA+AGG | - | chr5.4:73855293-73855312 | MS.gene64945:CDS | 15.0% |
| !!! | TTTTTCTTACTTTCATTTAC+AGG | + | chr5.4:73855180-73855199 | None:intergenic | 20.0% |
| ! | TAATATTTGTCACTACAATG+CGG | - | chr5.4:73854704-73854723 | MS.gene64945:CDS | 25.0% |
| AAGAAAAAAAGCTCACATGA+TGG | - | chr5.4:73855192-73855211 | MS.gene64945:intron | 30.0% | |
| AATATTATGGATGGTGATTC+TGG | - | chr5.4:73855105-73855124 | MS.gene64945:intron | 30.0% | |
| ATATTATGGATGGTGATTCT+GGG | - | chr5.4:73855106-73855125 | MS.gene64945:intron | 30.0% | |
| GCTACTCGTAAATCAAAATT+TGG | - | chr5.4:73855375-73855394 | MS.gene64945:CDS | 30.0% | |
| GTTGGCTGCTAAAAATATTA+CGG | - | chr5.4:73854987-73855006 | MS.gene64945:CDS | 30.0% | |
| TTGTGTGCTTCTATGAATAA+AGG | - | chr5.4:73854494-73854513 | MS.gene64945:CDS | 30.0% | |
| ! | GACTTTAAGAGATCATGATT+CGG | + | chr5.4:73855144-73855163 | None:intergenic | 30.0% |
| ! | GTGATAAAAAAGCACAAGTT+GGG | - | chr5.4:73854856-73854875 | MS.gene64945:CDS | 30.0% |
| ! | TGTGATAAAAAAGCACAAGT+TGG | - | chr5.4:73854855-73854874 | MS.gene64945:CDS | 30.0% |
| AAATATTACGGAGAGTAAGC+TGG | - | chr5.4:73854999-73855018 | MS.gene64945:CDS | 35.0% | |
| AGAAAGGCAATTGAAAAGCA+TGG | - | chr5.4:73855309-73855328 | MS.gene64945:CDS | 35.0% | |
| AGATCCTCAATATTGTTGGA+GGG | - | chr5.4:73854832-73854851 | MS.gene64945:CDS | 35.0% | |
| AGCTAGATCCTCAATATTGT+TGG | - | chr5.4:73854828-73854847 | MS.gene64945:CDS | 35.0% | |
| ATCCCAAGTACATAAGCTAA+CGG | + | chr5.4:73854770-73854789 | None:intergenic | 35.0% | |
| GCTGCTGAAAATATTATGGA+TGG | - | chr5.4:73855096-73855115 | MS.gene64945:intron | 35.0% | |
| GTTGGCTGCTGAAAATATTA+TGG | - | chr5.4:73855092-73855111 | MS.gene64945:intron | 35.0% | |
| TAAACAAAAAGTCATAGCCC+AGG | - | chr5.4:73855044-73855063 | MS.gene64945:CDS | 35.0% | |
| TAGATCCTCAATATTGTTGG+AGG | - | chr5.4:73854831-73854850 | MS.gene64945:CDS | 35.0% | |
| TATTATGGATGGTGATTCTG+GGG | - | chr5.4:73855107-73855126 | MS.gene64945:intron | 35.0% | |
| TGACTTGACTACTAAACAGA+AGG | - | chr5.4:73854927-73854946 | MS.gene64945:CDS | 35.0% | |
| AAATTTGCACCCATCCATTC+AGG | - | chr5.4:73855414-73855433 | MS.gene64945:CDS | 40.0% | |
| AATCCACAAAACTGCACACA+AGG | + | chr5.4:73854798-73854817 | None:intergenic | 40.0% | |
| AGGATAAGGCCATTACAATG+AGG | - | chr5.4:73854887-73854906 | MS.gene64945:CDS | 40.0% | |
| ATCCAGCTTCCTCATTGTAA+TGG | + | chr5.4:73854899-73854918 | None:intergenic | 40.0% | |
| CACACAATTTCTGACCAGTT+GGG | + | chr5.4:73854481-73854500 | None:intergenic | 40.0% | |
| CACTACAATGCGGTTATCTT+TGG | - | chr5.4:73854714-73854733 | MS.gene64945:CDS | 40.0% | |
| CCAAATAAAGCACGACAATC+AGG | - | chr5.4:73854523-73854542 | MS.gene64945:CDS | 40.0% | |
| TCACATGTACAGTGACAAGT+TGG | - | chr5.4:73854969-73854988 | MS.gene64945:CDS | 40.0% | |
| ! | CCTGATTGTCGTGCTTTATT+TGG | + | chr5.4:73854526-73854545 | None:intergenic | 40.0% |
| ! | TGCCGTTAGCTTATGTACTT+GGG | - | chr5.4:73854765-73854784 | MS.gene64945:CDS | 40.0% |
| ! | TTGCCGTTAGCTTATGTACT+TGG | - | chr5.4:73854764-73854783 | MS.gene64945:CDS | 40.0% |
| !! | TGGATGCTGATTCAGAGAAA+GGG | - | chr5.4:73854606-73854625 | MS.gene64945:CDS | 40.0% |
| !!! | GTGCTTTTTTACCTCACACT+TGG | + | chr5.4:73854459-73854478 | None:intergenic | 40.0% |
| AGGTCGACGATGCAGATAAT+CGG | - | chr5.4:73854543-73854562 | MS.gene64945:CDS | 45.0% | |
| CACACCCTCCAACAATATTG+AGG | + | chr5.4:73854839-73854858 | None:intergenic | 45.0% | |
| GCACACAATTTCTGACCAGT+TGG | + | chr5.4:73854482-73854501 | None:intergenic | 45.0% | |
| GGAGGCAATACACTTCTAAC+AGG | + | chr5.4:73854584-73854603 | None:intergenic | 45.0% | |
| GTTGGGAGATGTTGAGGATA+AGG | - | chr5.4:73854873-73854892 | MS.gene64945:CDS | 45.0% | |
| TAAACAGAAGGCAGTAGTCC+AGG | - | chr5.4:73854939-73854958 | MS.gene64945:CDS | 45.0% | |
| TCACATGTACGGTGACAAGT+TGG | - | chr5.4:73855074-73855093 | MS.gene64945:CDS | 45.0% | |
| TCTAACAGGCATGATCGACA+GGG | + | chr5.4:73854570-73854589 | None:intergenic | 45.0% | |
| TTCTAACAGGCATGATCGAC+AGG | + | chr5.4:73854571-73854590 | None:intergenic | 45.0% | |
| TTCTCTGAATCAGCATCCAG+TGG | + | chr5.4:73854605-73854624 | None:intergenic | 45.0% | |
| ! | GTTCCTTGTGTGCAGTTTTG+TGG | - | chr5.4:73854792-73854811 | MS.gene64945:CDS | 45.0% |
| ! | TAGAAGTGTATTGCCTCCAC+TGG | - | chr5.4:73854586-73854605 | MS.gene64945:CDS | 45.0% |
| !! | CTGGATGCTGATTCAGAGAA+AGG | - | chr5.4:73854605-73854624 | MS.gene64945:CDS | 45.0% |
| !! | GAGGTAAAAAAGCACCCAAC+TGG | - | chr5.4:73854464-73854483 | MS.gene64945:CDS | 45.0% |
| AAAGTCATAGCCCAGGAGCA+TGG | - | chr5.4:73855051-73855070 | MS.gene64945:CDS | 50.0% | |
| AGCCAACCACCTGAATGGAT+GGG | + | chr5.4:73855426-73855445 | None:intergenic | 50.0% | |
| CCAGGAGACTCAAGACAAAC+AGG | - | chr5.4:73855224-73855243 | MS.gene64945:intron | 50.0% | |
| CCTGTTTGTCTTGAGTCTCC+TGG | + | chr5.4:73855227-73855246 | None:intergenic | 50.0% | |
| GGCCATTACAATGAGGAAGC+TGG | - | chr5.4:73854894-73854913 | MS.gene64945:CDS | 50.0% | |
| GTACATGTGACCATGCTCCT+GGG | + | chr5.4:73854959-73854978 | None:intergenic | 50.0% | |
| TCTGAATCAGCATCCAGTGG+AGG | + | chr5.4:73854602-73854621 | None:intergenic | 50.0% | |
| TGTACATGTGACCATGCTCC+TGG | + | chr5.4:73854960-73854979 | None:intergenic | 50.0% | |
| TTTGCACCCATCCATTCAGG+TGG | - | chr5.4:73855417-73855436 | MS.gene64945:CDS | 50.0% | |
| ! | ACATGATGGTAACGCTGACC+AGG | - | chr5.4:73855206-73855225 | MS.gene64945:intron | 50.0% |
| ! | CAGGAGCATGGTCACATGTA+CGG | - | chr5.4:73854958-73854977 | MS.gene64945:CDS | 50.0% |
| ! | GCACAAGTTGGGAGATGTTG+AGG | - | chr5.4:73854867-73854886 | MS.gene64945:CDS | 50.0% |
| AAGGCAGTAGTCCAGGAGCA+TGG | - | chr5.4:73854946-73854965 | MS.gene64945:CDS | 55.0% | |
| CACCCATCCATTCAGGTGGT+TGG | - | chr5.4:73855421-73855440 | MS.gene64945:CDS | 55.0% | |
| CGAGTAGCTGCAAAACGAGC+TGG | + | chr5.4:73855363-73855382 | None:intergenic | 55.0% | |
| CGTACATGTGACCATGCTCC+TGG | + | chr5.4:73855065-73855084 | None:intergenic | 55.0% | |
| CTTCGAGCCAACCACCTGAA+TGG | + | chr5.4:73855431-73855450 | None:intergenic | 55.0% | |
| GAGCCAACCACCTGAATGGA+TGG | + | chr5.4:73855427-73855446 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 73854452 | 73855455 | 73854452 | ID=MS.gene64945 |
| chr5.4 | mRNA | 73854452 | 73855455 | 73854452 | ID=MS.gene64945.t1;Parent=MS.gene64945 |
| chr5.4 | exon | 73855280 | 73855455 | 73855280 | ID=MS.gene64945.t1.exon1;Parent=MS.gene64945.t1 |
| chr5.4 | CDS | 73855280 | 73855455 | 73855280 | ID=cds.MS.gene64945.t1;Parent=MS.gene64945.t1 |
| chr5.4 | exon | 73854452 | 73855100 | 73854452 | ID=MS.gene64945.t1.exon2;Parent=MS.gene64945.t1 |
| chr5.4 | CDS | 73854452 | 73855100 | 73854452 | ID=cds.MS.gene64945.t1;Parent=MS.gene64945.t1 |
| Gene Sequence |
| Protein sequence |
