Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65232.t1 | XP_013451608.1 | 93.4 | 425 | 27 | 1 | 1 | 424 | 77 | 501 | 8.80E-220 | 772.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65232.t1 | A0A072U8C6 | 93.4 | 425 | 27 | 1 | 1 | 424 | 77 | 501 | 6.4e-220 | 772.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052346 | MS.gene65232 | 0.801769 | 7.70E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene65232 | 0.830026 | 3.70E-55 | -1.69E-46 |
| MS.gene054927 | MS.gene65232 | 0.808026 | 3.79E-50 | -1.69E-46 |
| MS.gene055722 | MS.gene65232 | 0.804467 | 2.13E-49 | -1.69E-46 |
| MS.gene05601 | MS.gene65232 | 0.803606 | 3.22E-49 | -1.69E-46 |
| MS.gene056448 | MS.gene65232 | 0.812671 | 3.77E-51 | -1.69E-46 |
| MS.gene057734 | MS.gene65232 | 0.80119 | 1.01E-48 | -1.69E-46 |
| MS.gene060482 | MS.gene65232 | 0.80899 | 2.36E-50 | -1.69E-46 |
| MS.gene060837 | MS.gene65232 | 0.803196 | 3.91E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65232.t1 | MTR_6g033240 | 96.000 | 425 | 16 | 1 | 1 | 424 | 77 | 501 | 0.0 | 842 |
| MS.gene65232.t1 | MTR_7g084200 | 77.123 | 424 | 93 | 2 | 1 | 423 | 70 | 490 | 0.0 | 666 |
| MS.gene65232.t1 | MTR_2g101420 | 25.110 | 227 | 156 | 5 | 186 | 399 | 496 | 721 | 1.58e-19 | 91.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65232.t1 | AT3G19340 | 72.471 | 425 | 114 | 3 | 1 | 424 | 64 | 486 | 0.0 | 640 |
| MS.gene65232.t1 | AT5G13940 | 60.142 | 424 | 159 | 4 | 1 | 420 | 201 | 618 | 0.0 | 529 |
| MS.gene65232.t1 | AT5G13940 | 60.142 | 424 | 159 | 4 | 1 | 420 | 352 | 769 | 0.0 | 528 |
| MS.gene65232.t1 | AT2G46915 | 24.681 | 235 | 157 | 5 | 168 | 388 | 450 | 678 | 1.08e-18 | 89.0 |
| MS.gene65232.t1 | AT2G46915 | 23.952 | 167 | 118 | 3 | 230 | 388 | 469 | 634 | 8.48e-11 | 64.3 |
Find 72 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGATTAATGTTGCACTTTC+TGG | 0.122280 | 6.4:-6531988 | MS.gene65232:CDS |
| ACCATGTCTCTTTGATCTTT+TGG | 0.187810 | 6.4:+6531331 | None:intergenic |
| AAACATCCCAATGGCTGATA+TGG | 0.249732 | 6.4:-6530784 | MS.gene65232:CDS |
| CATCACTGTTAATGAATCTA+AGG | 0.255872 | 6.4:-6531944 | MS.gene65232:intron |
| ATAGGATCATTGTTGTTTAC+AGG | 0.256882 | 6.4:-6531065 | MS.gene65232:intron |
| GATTTCATTCTTTATTCTAA+TGG | 0.264326 | 6.4:-6516781 | MS.gene65232:CDS |
| CCACTGAAGAGCTTGTAATT+AGG | 0.272195 | 6.4:-6516339 | MS.gene65232:CDS |
| TCCTATCAAATGTCGGTTCT+TGG | 0.277732 | 6.4:+6531082 | None:intergenic |
| AAATGTCGGTTCTTGGATTA+AGG | 0.296141 | 6.4:+6531089 | None:intergenic |
| GTAGTTTCATCGTCCTTCTT+TGG | 0.297099 | 6.4:+6531296 | None:intergenic |
| GAATGACATCATCACATAAA+TGG | 0.305681 | 6.4:+6517303 | None:intergenic |
| AAAAGAGCAAATGAGATAAT+TGG | 0.324696 | 6.4:-6516367 | MS.gene65232:CDS |
| ATTCTTTATTCTAATGGAAC+AGG | 0.334205 | 6.4:-6516775 | MS.gene65232:CDS |
| GTTCACAAACTTGAGAAGTT+AGG | 0.346103 | 6.4:-6516565 | MS.gene65232:CDS |
| CACTGAAGAGCTTGTAATTA+GGG | 0.351958 | 6.4:-6516338 | MS.gene65232:CDS |
| AACGACGGATTACTTTATAA+TGG | 0.353329 | 6.4:-6531545 | MS.gene65232:CDS |
| ACAACAAGGCTTGACTTCTA+AGG | 0.356868 | 6.4:-6532386 | MS.gene65232:CDS |
| ATCTCTAAAATACCTCAAAA+AGG | 0.365680 | 6.4:+6532329 | None:intergenic |
| ACACATTGATATCGTCCTAT+TGG | 0.371910 | 6.4:+6516397 | None:intergenic |
| CGTATCCGCTATTGTTGGAT+TGG | 0.382873 | 6.4:-6530396 | MS.gene65232:intron |
| ACACGCTCATTGCACGTCTT+TGG | 0.384735 | 6.4:-6531513 | MS.gene65232:CDS |
| ACAACTGATTTGAGGGTTAT+CGG | 0.386134 | 6.4:-6530260 | MS.gene65232:CDS |
| ATCATATTTCGGCGTGGCAT+TGG | 0.417970 | 6.4:-6531577 | MS.gene65232:CDS |
| TCTTCCAGATTTCTATGATA+AGG | 0.424218 | 6.4:-6531746 | MS.gene65232:intron |
| GTTGGAATTCAGGATCCAAT+AGG | 0.426492 | 6.4:-6516412 | MS.gene65232:intron |
| ATTACCTTATCATAGAAATC+TGG | 0.430638 | 6.4:+6531742 | None:intergenic |
| AGCAGCTACAACTGATTTGA+GGG | 0.433861 | 6.4:-6530267 | MS.gene65232:CDS |
| TCCAAGAACCGACATTTGAT+AGG | 0.435282 | 6.4:-6531083 | MS.gene65232:CDS |
| TTTCTCGTATCCGCTATTGT+TGG | 0.447261 | 6.4:-6530401 | MS.gene65232:CDS |
| AAGCAGCTACAACTGATTTG+AGG | 0.451016 | 6.4:-6530268 | MS.gene65232:CDS |
| AATCAGTTGTAGCTGCTTGA+AGG | 0.465331 | 6.4:+6530273 | None:intergenic |
| TGAATTAAGGAATTGGATCA+AGG | 0.469465 | 6.4:-6516649 | MS.gene65232:intron |
| CTTAGATTCATTAACAGTGA+TGG | 0.474728 | 6.4:+6531945 | None:intergenic |
| CTATTTCCATATCAGCCATT+GGG | 0.484920 | 6.4:+6530778 | None:intergenic |
| ACTATTTCCATATCAGCCAT+TGG | 0.497926 | 6.4:+6530777 | None:intergenic |
| CTGGATTGACTCCAATGGAT+TGG | 0.499178 | 6.4:-6530430 | MS.gene65232:CDS |
| TGGATTGACTCCAATGGATT+GGG | 0.499880 | 6.4:-6530429 | MS.gene65232:CDS |
| AAATCTGGAAGATTATTAAA+CGG | 0.500190 | 6.4:+6531757 | None:intergenic |
| TTAGATTCATTAACAGTGAT+GGG | 0.504126 | 6.4:+6531946 | None:intergenic |
| GCTGTTCTTTCTACAGTAGT+CGG | 0.508253 | 6.4:-6530236 | MS.gene65232:CDS |
| TCCGATTCAAAACCAGAAAG+AGG | 0.513999 | 6.4:-6530828 | MS.gene65232:CDS |
| TTACTTTATAATGGAGAAAG+TGG | 0.514206 | 6.4:-6531536 | MS.gene65232:CDS |
| CAATCCATTGGAGTCAATCC+AGG | 0.515695 | 6.4:+6530431 | None:intergenic |
| CAGCCAGAAAAGAAAAGTCC+TGG | 0.519899 | 6.4:-6530449 | MS.gene65232:CDS |
| TAGAAATAACAAGACCTCAA+TGG | 0.529331 | 6.4:+6531221 | None:intergenic |
| AAACAACTAGACAGTGGAAA+GGG | 0.533025 | 6.4:-6517335 | MS.gene65232:CDS |
| AGAAACTTCACCCAATCCAT+TGG | 0.534790 | 6.4:+6530419 | None:intergenic |
| ATCAGTTGTAGCTGCTTGAA+GGG | 0.536884 | 6.4:+6530274 | None:intergenic |
| GAAGTTAGGAATTGTCACTA+AGG | 0.547265 | 6.4:-6516551 | MS.gene65232:intron |
| ATAGGACGATATCAATGTGT+TGG | 0.547411 | 6.4:-6516394 | MS.gene65232:CDS |
| CCTAATTACAAGCTCTTCAG+TGG | 0.547858 | 6.4:+6516339 | None:intergenic |
| CATATCTATTAAGAACCCTC+AGG | 0.558890 | 6.4:-6531489 | MS.gene65232:intron |
| AAGTCCTGGATTGACTCCAA+TGG | 0.567758 | 6.4:-6530435 | MS.gene65232:CDS |
| TTCTTTATTCTAATGGAACA+GGG | 0.587419 | 6.4:-6516774 | MS.gene65232:CDS |
| TAAGAAATTAGATCCAAAGA+AGG | 0.589628 | 6.4:-6531309 | MS.gene65232:CDS |
| GTCTTGAAAATATGCCATTG+AGG | 0.595428 | 6.4:-6531235 | MS.gene65232:intron |
| CTTGTAATTAGGGCAAAACA+AGG | 0.602992 | 6.4:-6516328 | MS.gene65232:CDS |
| ATCAAGATGATGTATATGAA+CGG | 0.607835 | 6.4:-6531262 | MS.gene65232:CDS |
| GAATTAAGGAATTGGATCAA+GGG | 0.615989 | 6.4:-6516648 | MS.gene65232:intron |
| ACAGGGAAAAGCTACAAGAC+AGG | 0.616213 | 6.4:-6516757 | MS.gene65232:intron |
| ACAATGATCCTATCAAATGT+CGG | 0.619773 | 6.4:+6531075 | None:intergenic |
| AATTACAAGCTCTTCAGTGG+TGG | 0.629317 | 6.4:+6516342 | None:intergenic |
| CAAACAACTAGACAGTGGAA+AGG | 0.633119 | 6.4:-6517336 | MS.gene65232:CDS |
| GGAATTGGATCAAGGGTGTG+AGG | 0.639992 | 6.4:-6516641 | MS.gene65232:CDS |
| GGACATAAGTTGGAACAACA+AGG | 0.645564 | 6.4:-6532400 | MS.gene65232:CDS |
| TCCAAAAGATCAAAGAGACA+TGG | 0.650268 | 6.4:-6531332 | MS.gene65232:CDS |
| ACTAACCAATCCAACAATAG+CGG | 0.662232 | 6.4:+6530391 | None:intergenic |
| CATTGGAATTGATCAAACGA+CGG | 0.663837 | 6.4:-6531560 | MS.gene65232:CDS |
| TATGACAAACAACTAGACAG+TGG | 0.680152 | 6.4:-6517341 | MS.gene65232:CDS |
| TAGTATATCATATTTCGGCG+TGG | 0.680985 | 6.4:-6531583 | MS.gene65232:CDS |
| GCATTTCAGAAACATCCCAA+TGG | 0.683421 | 6.4:-6530793 | MS.gene65232:CDS |
| GAAGGACGATGAAACTACGT+CGG | 0.718944 | 6.4:-6531291 | MS.gene65232:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 6516312 | 6532563 | 6516312 | ID=MS.gene65232 |
| chr6.4 | mRNA | 6516312 | 6532563 | 6516312 | ID=MS.gene65232.t1;Parent=MS.gene65232 |
| chr6.4 | exon | 6532558 | 6532563 | 6532558 | ID=MS.gene65232.t1.exon1;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6532558 | 6532563 | 6532558 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| chr6.4 | exon | 6532342 | 6532455 | 6532342 | ID=MS.gene65232.t1.exon2;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6532342 | 6532455 | 6532342 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| chr6.4 | exon | 6531945 | 6532049 | 6531945 | ID=MS.gene65232.t1.exon3;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6531945 | 6532049 | 6531945 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| chr6.4 | exon | 6531747 | 6531818 | 6531747 | ID=MS.gene65232.t1.exon4;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6531747 | 6531818 | 6531747 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| chr6.4 | exon | 6531490 | 6531602 | 6531490 | ID=MS.gene65232.t1.exon5;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6531490 | 6531602 | 6531490 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| chr6.4 | exon | 6531236 | 6531370 | 6531236 | ID=MS.gene65232.t1.exon6;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6531236 | 6531370 | 6531236 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| chr6.4 | exon | 6531066 | 6531134 | 6531066 | ID=MS.gene65232.t1.exon7;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6531066 | 6531134 | 6531066 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| chr6.4 | exon | 6530773 | 6530860 | 6530773 | ID=MS.gene65232.t1.exon8;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6530773 | 6530860 | 6530773 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| chr6.4 | exon | 6530397 | 6530468 | 6530397 | ID=MS.gene65232.t1.exon9;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6530397 | 6530468 | 6530397 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| chr6.4 | exon | 6530212 | 6530312 | 6530212 | ID=MS.gene65232.t1.exon10;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6530212 | 6530312 | 6530212 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| chr6.4 | exon | 6517295 | 6517412 | 6517295 | ID=MS.gene65232.t1.exon11;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6517295 | 6517412 | 6517295 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| chr6.4 | exon | 6516758 | 6516817 | 6516758 | ID=MS.gene65232.t1.exon12;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6516758 | 6516817 | 6516758 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| chr6.4 | exon | 6516552 | 6516662 | 6516552 | ID=MS.gene65232.t1.exon13;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6516552 | 6516662 | 6516552 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| chr6.4 | exon | 6516312 | 6516422 | 6516312 | ID=MS.gene65232.t1.exon14;Parent=MS.gene65232.t1 |
| chr6.4 | CDS | 6516312 | 6516422 | 6516312 | ID=cds.MS.gene65232.t1;Parent=MS.gene65232.t1 |
| Gene Sequence |
| Protein sequence |
