Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65711.t1 | XP_003614713.1 | 97.1 | 139 | 4 | 0 | 54 | 192 | 27 | 165 | 4.30E-72 | 281.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65711.t1 | Q6IDL4 | 64.1 | 142 | 48 | 2 | 54 | 192 | 29 | 170 | 1.3e-48 | 194.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65711.t1 | G7JXB5 | 97.1 | 139 | 4 | 0 | 54 | 192 | 27 | 165 | 3.1e-72 | 281.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene65711 | 0.800816 | 1.21E-48 | -1.69E-46 |
| MS.gene051832 | MS.gene65711 | 0.812303 | 4.54E-51 | -1.69E-46 |
| MS.gene052016 | MS.gene65711 | 0.801965 | 7.02E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene65711 | 0.815466 | 9.11E-52 | -1.69E-46 |
| MS.gene053786 | MS.gene65711 | 0.849406 | 3.30E-60 | -1.69E-46 |
| MS.gene054576 | MS.gene65711 | 0.83271 | 8.09E-56 | -1.69E-46 |
| MS.gene055513 | MS.gene65711 | 0.822285 | 2.57E-53 | -1.69E-46 |
| MS.gene056403 | MS.gene65711 | 0.800927 | 1.15E-48 | -1.69E-46 |
| MS.gene057890 | MS.gene65711 | -0.800656 | 1.30E-48 | -1.69E-46 |
| MS.gene059170 | MS.gene65711 | 0.845094 | 5.03E-59 | -1.69E-46 |
| MS.gene061100 | MS.gene65711 | 0.818104 | 2.33E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65711.t1 | MTR_5g058140 | 97.122 | 139 | 4 | 0 | 54 | 192 | 27 | 165 | 5.89e-100 | 289 |
| MS.gene65711.t1 | MTR_3g072600 | 70.552 | 163 | 41 | 4 | 36 | 192 | 9 | 170 | 3.29e-75 | 226 |
| MS.gene65711.t1 | MTR_2g007210 | 68.085 | 141 | 43 | 2 | 54 | 192 | 19 | 159 | 3.24e-66 | 203 |
| MS.gene65711.t1 | MTR_8g088940 | 68.085 | 141 | 42 | 2 | 55 | 192 | 24 | 164 | 5.76e-57 | 180 |
| MS.gene65711.t1 | MTR_8g088980 | 71.134 | 97 | 27 | 1 | 97 | 192 | 19 | 115 | 6.83e-46 | 150 |
| MS.gene65711.t1 | MTR_4g082315 | 41.379 | 145 | 79 | 2 | 54 | 192 | 25 | 169 | 2.15e-38 | 132 |
| MS.gene65711.t1 | MTR_6g012770 | 38.926 | 149 | 84 | 3 | 51 | 192 | 17 | 165 | 7.83e-34 | 120 |
| MS.gene65711.t1 | MTR_7g011490 | 35.135 | 148 | 90 | 2 | 51 | 192 | 18 | 165 | 3.01e-30 | 111 |
| MS.gene65711.t1 | MTR_5g042270 | 34.375 | 128 | 83 | 1 | 66 | 192 | 11 | 138 | 2.01e-24 | 95.9 |
| MS.gene65711.t1 | MTR_5g042260 | 34.014 | 147 | 89 | 4 | 54 | 192 | 26 | 172 | 1.19e-23 | 94.7 |
| MS.gene65711.t1 | MTR_5g042320 | 33.784 | 148 | 89 | 4 | 54 | 192 | 29 | 176 | 8.95e-19 | 81.3 |
| MS.gene65711.t1 | MTR_5g042320 | 34.459 | 148 | 88 | 4 | 54 | 192 | 29 | 176 | 4.12e-18 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65711.t1 | AT1G09580 | 64.085 | 142 | 48 | 2 | 54 | 192 | 29 | 170 | 7.88e-64 | 197 |
| MS.gene65711.t1 | AT1G21900 | 61.074 | 149 | 53 | 3 | 49 | 192 | 21 | 169 | 4.24e-62 | 193 |
| MS.gene65711.t1 | AT1G57620 | 55.696 | 158 | 66 | 3 | 38 | 192 | 9 | 165 | 4.10e-59 | 186 |
| MS.gene65711.t1 | AT3G10780 | 52.778 | 144 | 61 | 3 | 54 | 190 | 25 | 168 | 4.32e-43 | 145 |
| MS.gene65711.t1 | AT1G26690 | 40.690 | 145 | 80 | 1 | 54 | 192 | 23 | 167 | 1.37e-36 | 128 |
| MS.gene65711.t1 | AT1G69460 | 40.132 | 152 | 77 | 3 | 55 | 192 | 16 | 167 | 1.09e-32 | 118 |
| MS.gene65711.t1 | AT3G29070 | 37.931 | 145 | 84 | 2 | 54 | 192 | 34 | 178 | 8.13e-32 | 116 |
| MS.gene65711.t1 | AT1G14010 | 35.802 | 162 | 90 | 4 | 60 | 215 | 27 | 180 | 2.09e-31 | 114 |
| MS.gene65711.t1 | AT2G03040 | 36.429 | 140 | 82 | 1 | 60 | 192 | 27 | 166 | 1.09e-29 | 108 |
| MS.gene65711.t1 | AT2G03290 | 35.714 | 140 | 83 | 1 | 60 | 192 | 27 | 166 | 5.36e-28 | 105 |
Find 57 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATTTCAGGTTCTGTTTGC+TGG | 0.215183 | 5.2:+49343783 | None:intergenic |
| CCTCCTCCACGCCGGAAGTT+AGG | 0.260075 | 5.2:+49343673 | None:intergenic |
| CTCCTCCACGCCGGAAGTTA+GGG | 0.298128 | 5.2:+49343674 | None:intergenic |
| GAAATTATGTGGCATGCTTT+TGG | 0.304500 | 5.2:-49337385 | MS.gene65711:CDS |
| CCGCTGCTGCCACTGTTTCC+CGG | 0.321341 | 5.2:+49343716 | None:intergenic |
| TTCTTAAAACAGAAGCTATA+TGG | 0.325494 | 5.2:-49340934 | MS.gene65711:intron |
| GTCAGCCTTGATTGGAAAAC+TGG | 0.328485 | 5.2:-49337329 | MS.gene65711:CDS |
| ACGAGGCACTCCTATTGATT+AGG | 0.336153 | 5.2:-49336172 | None:intergenic |
| TTGCAGGTAACATCTCCTTT+TGG | 0.356646 | 5.2:-49337473 | MS.gene65711:intron |
| CTGACACTTACACTACCTTC+TGG | 0.357413 | 5.2:+49337347 | None:intergenic |
| CGATTATCTAATTTATTTGA+AGG | 0.366207 | 5.2:-49337099 | MS.gene65711:CDS |
| GGTGGTGGTCGGTGGATCTC+TGG | 0.369812 | 5.2:+49343804 | None:intergenic |
| TGGAATTTCAGCAAAGGATT+GGG | 0.395382 | 5.2:-49337309 | MS.gene65711:CDS |
| CTGGAATTTCAGCAAAGGAT+TGG | 0.405549 | 5.2:-49337310 | MS.gene65711:CDS |
| CCAGCAAACAGAACCTGAAA+TGG | 0.408502 | 5.2:-49343783 | MS.gene65711:CDS |
| AGGGTGTTGAACTTGAAATC+AGG | 0.420862 | 5.2:-49337151 | MS.gene65711:CDS |
| CGAGGCACTCCTATTGATTA+GGG | 0.437012 | 5.2:-49336171 | None:intergenic |
| TGGTTGACCATGCCTAGTTC+AGG | 0.442380 | 5.2:-49340914 | MS.gene65711:CDS |
| TGTTAGGCGCCCTAATCAAT+AGG | 0.448051 | 5.2:+49336162 | None:intergenic |
| AATAATAAAACATACCTTTG+CGG | 0.453832 | 5.2:+49340802 | None:intergenic |
| CAGGTTCTGTTTGCTGGTGG+TGG | 0.455282 | 5.2:+49343789 | None:intergenic |
| AACGCAGACAAACAAGCCAC+CGG | 0.455763 | 5.2:-49343735 | MS.gene65711:CDS |
| TGGTGAACGTTGTTTCCAAA+AGG | 0.464734 | 5.2:+49337458 | None:intergenic |
| CAGGAAGCTCGAAGGAATAG+TGG | 0.469265 | 5.2:-49337132 | MS.gene65711:CDS |
| GGTGGCTTGTTTGTCTGCGT+TGG | 0.473386 | 5.2:+49343737 | None:intergenic |
| GTGTAAGTGTCAGCCTTGAT+TGG | 0.480040 | 5.2:-49337337 | MS.gene65711:CDS |
| TTCTGTTTGCTGGTGGTGGT+CGG | 0.487533 | 5.2:+49343793 | None:intergenic |
| GAGAATCTAGTTGAGGCAAA+AGG | 0.493384 | 5.2:-49336231 | MS.gene65711:CDS |
| ACACATTTAGTTCCTGAACT+AGG | 0.497691 | 5.2:+49340902 | None:intergenic |
| TACTATGTTGTAACTGATGA+AGG | 0.503160 | 5.2:-49340839 | MS.gene65711:CDS |
| CCGGGAAACAGTGGCAGCAG+CGG | 0.509887 | 5.2:-49343716 | MS.gene65711:CDS |
| TGACACTTACACTACCTTCT+GGG | 0.510439 | 5.2:+49337348 | None:intergenic |
| TGCCACATAATTTCCACTCT+CGG | 0.522318 | 5.2:+49337394 | None:intergenic |
| CAACGTCAACCCCTAACTTC+CGG | 0.522683 | 5.2:-49343684 | MS.gene65711:CDS |
| GTGGTCGGTGGATCTCTGGA+TGG | 0.528723 | 5.2:+49343808 | None:intergenic |
| AGTTGAGGCAAAAGGAAACT+TGG | 0.530434 | 5.2:-49336223 | MS.gene65711:CDS |
| TCAATAGGAGTGCCTCGTCT+TGG | 0.538524 | 5.2:+49336177 | None:intergenic |
| GAAAACTGGAATTTCAGCAA+AGG | 0.546642 | 5.2:-49337315 | MS.gene65711:CDS |
| GCATTTACAACTACCGAGAG+TGG | 0.562957 | 5.2:-49337407 | MS.gene65711:CDS |
| TCAAATAAATTAGATAATCG+TGG | 0.574741 | 5.2:+49337102 | None:intergenic |
| ACGCAGACAAACAAGCCACC+GGG | 0.581310 | 5.2:-49343734 | MS.gene65711:CDS |
| ATAAATTAGATAATCGTGGA+TGG | 0.583572 | 5.2:+49337106 | None:intergenic |
| TGTTTGCTGGTGGTGGTCGG+TGG | 0.584134 | 5.2:+49343796 | None:intergenic |
| TTTCAGGTTCTGTTTGCTGG+TGG | 0.584975 | 5.2:+49343786 | None:intergenic |
| TACCGAGAGTGGAAATTATG+TGG | 0.589093 | 5.2:-49337396 | MS.gene65711:CDS |
| GTCGGTGGATCTCTGGATGG+TGG | 0.596297 | 5.2:+49343811 | None:intergenic |
| TCTTCACACTATTTCCGCAA+AGG | 0.600461 | 5.2:-49340816 | MS.gene65711:intron |
| TCCTCCACGCCGGAAGTTAG+GGG | 0.601100 | 5.2:+49343675 | None:intergenic |
| AGGAACTAAATGTGTGTCTG+AGG | 0.602454 | 5.2:-49340894 | MS.gene65711:CDS |
| TTTAGTTCCTGAACTAGGCA+TGG | 0.606427 | 5.2:+49340907 | None:intergenic |
| AACAAGCCACCGGGAAACAG+TGG | 0.610862 | 5.2:-49343725 | MS.gene65711:CDS |
| CTGCTGCCACTGTTTCCCGG+TGG | 0.616361 | 5.2:+49343719 | None:intergenic |
| CTTGAAATCAGGAAGCTCGA+AGG | 0.630601 | 5.2:-49337140 | MS.gene65711:CDS |
| CATAATGAAAATGTTACACA+AGG | 0.640271 | 5.2:-49337437 | MS.gene65711:CDS |
| TGCAAAGAAAGAAAAGATTG+AGG | 0.640940 | 5.2:-49337279 | MS.gene65711:intron |
| ATGGAAGGAAAACACCCAGA+AGG | 0.668528 | 5.2:-49337362 | MS.gene65711:CDS |
| ACAATCAAGAAACCAAGACG+AGG | 0.736180 | 5.2:-49336189 | MS.gene65711:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 49336173 | 49343842 | 49336173 | ID=MS.gene65711 |
| chr5.2 | mRNA | 49336173 | 49343842 | 49336173 | ID=MS.gene65711.t1;Parent=MS.gene65711 |
| chr5.2 | exon | 49343683 | 49343842 | 49343683 | ID=MS.gene65711.t1.exon1;Parent=MS.gene65711.t1 |
| chr5.2 | CDS | 49343683 | 49343842 | 49343683 | ID=cds.MS.gene65711.t1;Parent=MS.gene65711.t1 |
| chr5.2 | exon | 49340817 | 49340944 | 49340817 | ID=MS.gene65711.t1.exon2;Parent=MS.gene65711.t1 |
| chr5.2 | CDS | 49340817 | 49340944 | 49340817 | ID=cds.MS.gene65711.t1;Parent=MS.gene65711.t1 |
| chr5.2 | exon | 49337280 | 49337489 | 49337280 | ID=MS.gene65711.t1.exon3;Parent=MS.gene65711.t1 |
| chr5.2 | CDS | 49337280 | 49337489 | 49337280 | ID=cds.MS.gene65711.t1;Parent=MS.gene65711.t1 |
| chr5.2 | exon | 49337095 | 49337171 | 49337095 | ID=MS.gene65711.t1.exon4;Parent=MS.gene65711.t1 |
| chr5.2 | CDS | 49337095 | 49337171 | 49337095 | ID=cds.MS.gene65711.t1;Parent=MS.gene65711.t1 |
| chr5.2 | exon | 49336173 | 49336275 | 49336173 | ID=MS.gene65711.t1.exon5;Parent=MS.gene65711.t1 |
| chr5.2 | CDS | 49336173 | 49336275 | 49336173 | ID=cds.MS.gene65711.t1;Parent=MS.gene65711.t1 |
| Gene Sequence |
| Protein sequence |
