AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66586


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66586.t1 MTR_6g464280 96.209 211 8 0 1 211 108 318 3.31e-150 420
MS.gene66586.t1 MTR_6g464320 85.782 211 30 0 1 211 109 319 1.83e-132 375
MS.gene66586.t1 MTR_6g464510 72.512 211 57 1 1 211 111 320 4.78e-110 318
MS.gene66586.t1 MTR_1g102160 54.460 213 92 2 1 209 106 317 7.40e-76 231
MS.gene66586.t1 MTR_0007s0520 54.327 208 91 3 1 205 106 312 6.09e-72 221
MS.gene66586.t1 MTR_8g035520 52.752 218 90 4 1 211 107 318 6.24e-71 219
MS.gene66586.t1 MTR_1g102170 55.051 198 88 1 1 198 123 319 3.78e-70 217
MS.gene66586.t1 MTR_8g035540 51.835 218 92 4 1 211 99 310 2.78e-68 212
MS.gene66586.t1 MTR_6g464120 53.846 208 93 3 1 207 110 315 2.15e-66 213
MS.gene66586.t1 MTR_0007s0480 54.430 158 68 3 51 205 2 158 1.21e-51 164
MS.gene66586.t1 MTR_8g035500 38.889 216 121 3 1 211 102 311 4.80e-48 160
MS.gene66586.t1 MTR_6g065430 41.848 184 98 4 4 186 120 295 5.73e-40 139
MS.gene66586.t1 MTR_7g406880 41.117 197 95 8 6 186 122 313 1.21e-34 126
MS.gene66586.t1 MTR_6g065120 39.106 179 102 3 5 183 129 300 1.90e-33 122
MS.gene66586.t1 MTR_1g111280 38.679 212 113 4 5 205 120 325 4.13e-33 122
MS.gene66586.t1 MTR_6g065270 39.106 179 102 4 5 183 129 300 6.22e-33 121
MS.gene66586.t1 MTR_6g065400 39.106 179 102 3 5 183 127 298 1.65e-32 120
MS.gene66586.t1 MTR_6g065110 37.989 179 104 3 5 183 128 299 2.00e-31 117
MS.gene66586.t1 MTR_7g107040 38.049 205 112 6 1 199 97 292 2.31e-31 117
MS.gene66586.t1 MTR_6g065370 37.430 179 105 3 5 183 127 298 2.70e-30 114
MS.gene66586.t1 MTR_4g050140 35.556 225 127 7 1 211 96 316 3.73e-28 108
MS.gene66586.t1 MTR_6g065340 34.973 183 96 4 1 183 134 293 8.24e-26 102
MS.gene66586.t1 MTR_4g086510 33.333 213 120 7 1 204 103 302 6.24e-25 99.8
MS.gene66586.t1 MTR_7g056207 33.178 214 125 7 1 207 135 337 1.49e-23 96.7
MS.gene66586.t1 MTR_1g089310 36.702 188 105 6 1 186 133 308 1.02e-22 94.4
MS.gene66586.t1 MTR_1g089310 36.702 188 105 6 1 186 149 324 1.29e-22 94.0
MS.gene66586.t1 MTR_4g086480 34.975 203 109 8 11 204 115 303 4.94e-22 92.0
MS.gene66586.t1 MTR_1g012430 32.961 179 113 4 6 183 98 270 8.89e-22 91.3
MS.gene66586.t1 MTR_7g093950 33.854 192 110 5 1 186 134 314 3.48e-21 90.1
MS.gene66586.t1 MTR_3g012440 34.752 141 85 2 1 136 116 254 6.81e-20 86.3
MS.gene66586.t1 MTR_1g082210 36.232 138 80 3 1 137 133 263 2.32e-19 85.1
MS.gene66586.t1 MTR_4g086470 34.270 178 100 6 11 183 114 279 1.44e-18 82.4
MS.gene66586.t1 MTR_6g022710 29.592 196 98 5 1 156 188 383 3.02e-18 82.4
MS.gene66586.t1 MTR_3g101420 32.895 228 105 9 8 211 105 308 2.73e-17 79.0
MS.gene66586.t1 MTR_4g086540 30.244 205 111 6 8 208 117 293 3.21e-17 78.6
MS.gene66586.t1 MTR_1g105020 41.441 111 57 2 1 103 101 211 1.41e-16 77.0
MS.gene66586.t1 MTR_3g450680 31.308 214 115 8 1 202 109 302 4.07e-16 75.9
MS.gene66586.t1 MTR_1g104930 37.500 96 52 2 6 94 106 200 5.81e-15 72.4
MS.gene66586.t1 MTR_5g005850 29.279 222 127 9 8 211 113 322 1.34e-14 71.6
MS.gene66586.t1 MTR_7g051020 38.144 97 48 3 7 94 110 203 1.46e-14 71.2
MS.gene66586.t1 MTR_1g104933 37.755 98 49 3 6 94 104 198 4.53e-14 70.1
MS.gene66586.t1 MTR_8g040860 27.907 215 118 9 1 202 109 299 1.05e-13 68.9
MS.gene66586.t1 MTR_5g005920 28.505 214 120 12 1 202 109 301 1.99e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66586.t1 AT5G62180 54.067 209 95 1 1 209 108 315 3.39e-76 232
MS.gene66586.t1 AT2G45600 46.188 223 101 4 1 211 94 309 1.31e-60 192
MS.gene66586.t1 AT2G45610 43.868 212 107 3 1 206 107 312 2.53e-54 176
MS.gene66586.t1 AT5G16080 37.436 195 104 5 1 186 120 305 3.51e-31 117
MS.gene66586.t1 AT5G23530 35.644 202 122 4 1 201 115 309 4.91e-31 116
MS.gene66586.t1 AT1G68620 38.172 186 102 6 6 184 122 301 2.57e-27 106
MS.gene66586.t1 AT5G06570 35.185 216 121 7 1 205 104 311 2.04e-26 103
MS.gene66586.t1 AT5G06570 35.185 216 121 7 1 205 104 311 2.04e-26 103
MS.gene66586.t1 AT5G06570 35.185 216 121 7 1 205 119 326 2.54e-26 103
MS.gene66586.t1 AT5G06570 35.185 216 121 7 1 205 119 326 2.54e-26 103
MS.gene66586.t1 AT1G19190 33.333 192 99 4 3 183 102 275 5.92e-22 91.7
MS.gene66586.t1 AT3G63010 36.111 180 103 4 8 186 141 309 1.91e-21 90.9
MS.gene66586.t1 AT1G47480 30.918 207 126 5 1 203 100 293 4.26e-21 89.4
MS.gene66586.t1 AT5G27320 42.188 128 66 3 7 133 138 258 1.34e-20 88.6
MS.gene66586.t1 AT1G49640 44.086 93 49 2 4 96 105 194 2.70e-20 87.0
MS.gene66586.t1 AT5G14310 30.675 163 76 1 1 126 179 341 2.10e-18 83.2
MS.gene66586.t1 AT3G05120 38.806 134 74 3 5 137 138 264 1.51e-17 80.1
MS.gene66586.t1 AT3G48700 42.268 97 48 2 6 94 107 203 2.86e-17 79.0
MS.gene66586.t1 AT3G27320 27.228 202 99 6 1 156 162 361 7.51e-17 78.6
MS.gene66586.t1 AT3G27320 27.228 202 99 6 1 156 194 393 7.87e-17 78.6
MS.gene66586.t1 AT3G48690 40.000 95 50 2 7 94 105 199 5.85e-15 72.4
MS.gene66586.t1 AT1G49650 35.714 98 55 3 4 96 159 253 7.09e-13 67.0
MS.gene66586.t1 AT1G49660 37.000 100 56 3 4 98 103 200 1.04e-12 66.2

Find 51 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAATGAAATTCTTTGCTT+TGG 0.269748 6.4:+39771566 None:intergenic
ATCATGAGTATTCCAATCTA+AGG 0.274329 6.4:-39771782 MS.gene66586:CDS
CTTGAGCCGTTGAAAATTCT+AGG 0.274915 6.4:-39771942 MS.gene66586:CDS
TAAGGAGTTGGTTAAATTGT+TGG 0.354633 6.4:-39771661 MS.gene66586:CDS
TCCATTGGTTGATCGTAATA+AGG 0.354913 6.4:-39771679 MS.gene66586:CDS
TGGAAGCATTGAGTTTGATT+AGG 0.366794 6.4:-39772088 MS.gene66586:CDS
ACCATTCCCAACCCTTAGAT+TGG 0.372685 6.4:+39771770 None:intergenic
ATGAAATTCTTTGCTTTGGA+TGG 0.390861 6.4:+39771570 None:intergenic
GGTTAAATTGTTGGAGGAGA+AGG 0.419389 6.4:-39771652 MS.gene66586:CDS
TGATCACGTTTAACACCAAT+TGG 0.432249 6.4:+39771801 None:intergenic
GCCGCCGGTAGCCGGTGCTC+CGG 0.443546 6.4:+39772125 None:intergenic
TAACTCTGACTCAGTCCTAT+TGG 0.451589 6.4:+39771887 None:intergenic
CATCATATGCCGCCGGTAGC+CGG 0.451594 6.4:+39772117 None:intergenic
AGGGTGTTGGTGAGTATGAA+TGG 0.462381 6.4:-39771708 MS.gene66586:CDS
GAAGGGTGTGGAAGTGGTGA+AGG 0.466496 6.4:-39771634 MS.gene66586:CDS
AGTATTCCAATCTAAGGGTT+GGG 0.474229 6.4:-39771776 MS.gene66586:CDS
GTTAAATTGTTGGAGGAGAA+GGG 0.476633 6.4:-39771651 MS.gene66586:CDS
CGCACCGGAGCACCGGCTAC+CGG 0.478223 6.4:-39772129 MS.gene66586:CDS
GGAGGAGAAGGGTGTGGAAG+TGG 0.478996 6.4:-39771640 MS.gene66586:CDS
TTTCTAATGGGAAATAGTGC+TGG 0.493645 6.4:-39772017 MS.gene66586:CDS
ATCGCCTCGCACCGGAGCAC+CGG 0.497130 6.4:-39772136 MS.gene66586:CDS
GTGTGTTAGTGATTTGATGT+GGG 0.504249 6.4:-39771835 MS.gene66586:CDS
GGTTGATCGTAATAAGGAGT+TGG 0.507526 6.4:-39771673 MS.gene66586:CDS
TGGGAGTTAGCGTTACCAAT+TGG 0.507682 6.4:-39771816 MS.gene66586:CDS
CGTCGAAGCAGTTGTCGCCT+CGG 0.508826 6.4:-39772165 MS.gene66586:CDS
GGAGTTGGTTAAATTGTTGG+AGG 0.511533 6.4:-39771658 MS.gene66586:CDS
ATTGTTGGAGGAGAAGGGTG+TGG 0.514825 6.4:-39771646 MS.gene66586:CDS
CGAGGCGACAACTGCTTCGA+CGG 0.523045 6.4:+39772166 None:intergenic
AGGACTACGTGTAGTTCAAA+AGG 0.533022 6.4:-39771973 MS.gene66586:CDS
TGTGTGTTAGTGATTTGATG+TGG 0.546036 6.4:-39771836 MS.gene66586:CDS
TTGAGCCGTTGAAAATTCTA+GGG 0.552613 6.4:-39771941 MS.gene66586:CDS
GAGTATTCCAATCTAAGGGT+TGG 0.553194 6.4:-39771777 MS.gene66586:CDS
CAAAGCAAAGAATTTCATTG+AGG 0.554638 6.4:-39771565 None:intergenic
GGTCGATTATCGCCTCGCAC+CGG 0.575927 6.4:-39772144 MS.gene66586:CDS
GTAGCCGGTGCTCCGGTGCG+AGG 0.576122 6.4:+39772132 None:intergenic
GCTACAATTGCATATCATGC+AGG 0.576937 6.4:-39771993 MS.gene66586:CDS
GGCGGCATATGATGATGCTA+TGG 0.579015 6.4:-39772108 MS.gene66586:CDS
TTAGGTCAAGTGATGATGAA+TGG 0.579905 6.4:-39772070 MS.gene66586:CDS
TCCAATCTAAGGGTTGGGAA+TGG 0.580920 6.4:-39771771 MS.gene66586:CDS
GGATCATGAGTGGAGGGTGT+TGG 0.586722 6.4:-39771721 MS.gene66586:CDS
TATGAATGGTGGAGATCCAT+TGG 0.590004 6.4:-39771694 MS.gene66586:CDS
ACCGGAGCACCGGCTACCGG+CGG 0.597839 6.4:-39772126 MS.gene66586:CDS
AGATTAAGGATCATGAGTGG+AGG 0.598392 6.4:-39771728 MS.gene66586:CDS
GATTAAGGATCATGAGTGGA+GGG 0.601559 6.4:-39771727 MS.gene66586:CDS
TCATGAGTATTCCAATCTAA+GGG 0.620597 6.4:-39771781 MS.gene66586:CDS
ATCAAATCACTAACACACAA+AGG 0.629463 6.4:+39771840 None:intergenic
TCCTTATTACGATCAACCAA+TGG 0.629948 6.4:+39771678 None:intergenic
CTAAGATTAAGGATCATGAG+TGG 0.641493 6.4:-39771731 MS.gene66586:CDS
ATAGCATCATCATATGCCGC+CGG 0.642105 6.4:+39772110 None:intergenic
GTGTTGGTGAGTATGAATGG+TGG 0.677117 6.4:-39771705 MS.gene66586:CDS
TGCGAGGCGATAATCGACCG+AGG 0.723884 6.4:+39772148 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCAAAATGTTTTCTAAT+GGG - chr6.4:39771713-39771732 MS.gene66586:CDS 15.0%
!!! CTTTTCAAAATGTTTTCTAA+TGG - chr6.4:39771712-39771731 MS.gene66586:CDS 20.0%
!!! TGAAAAACTTGCTAAGATTA+AGG - chr6.4:39772000-39772019 MS.gene66586:CDS 25.0%
ATCAAATCACTAACACACAA+AGG + chr6.4:39771905-39771924 None:intergenic 30.0%
ATCATGAGTATTCCAATCTA+AGG - chr6.4:39771960-39771979 MS.gene66586:CDS 30.0%
CTCAATGAAATTCTTTGCTT+TGG + chr6.4:39772179-39772198 None:intergenic 30.0%
TCATGAGTATTCCAATCTAA+GGG - chr6.4:39771961-39771980 MS.gene66586:CDS 30.0%
! ATCATCCCTAGAATTTTCAA+CGG + chr6.4:39771809-39771828 None:intergenic 30.0%
! ATGAAATTCTTTGCTTTGGA+TGG + chr6.4:39772175-39772194 None:intergenic 30.0%
! TAAGGAGTTGGTTAAATTGT+TGG - chr6.4:39772081-39772100 MS.gene66586:CDS 30.0%
! TTCAAGAAGATGGTTTTCAT+GGG - chr6.4:39772134-39772153 MS.gene66586:CDS 30.0%
! TTTCAAGAAGATGGTTTTCA+TGG - chr6.4:39772133-39772152 MS.gene66586:CDS 30.0%
!!! ATATTGCGTCAACCATTTTT+TGG - chr6.4:39771827-39771846 MS.gene66586:CDS 30.0%
AGTATTCCAATCTAAGGGTT+GGG - chr6.4:39771966-39771985 MS.gene66586:CDS 35.0%
CTAACACACAAAGGAAAAAC+AGG + chr6.4:39771896-39771915 None:intergenic 35.0%
CTAAGATTAAGGATCATGAG+TGG - chr6.4:39772011-39772030 MS.gene66586:CDS 35.0%
GTGTGTTAGTGATTTGATGT+GGG - chr6.4:39771907-39771926 MS.gene66586:CDS 35.0%
GTTAAATTGTTGGAGGAGAA+GGG - chr6.4:39772091-39772110 MS.gene66586:CDS 35.0%
TCCTTATTACGATCAACCAA+TGG + chr6.4:39772067-39772086 None:intergenic 35.0%
TGATCACGTTTAACACCAAT+TGG + chr6.4:39771944-39771963 None:intergenic 35.0%
TGTGTGTTAGTGATTTGATG+TGG - chr6.4:39771906-39771925 MS.gene66586:CDS 35.0%
TTGAGCCGTTGAAAATTCTA+GGG - chr6.4:39771801-39771820 MS.gene66586:CDS 35.0%
TTTCTAATGGGAAATAGTGC+TGG - chr6.4:39771725-39771744 MS.gene66586:CDS 35.0%
! TCAAGAAGATGGTTTTCATG+GGG - chr6.4:39772135-39772154 MS.gene66586:CDS 35.0%
! TCCATTGGTTGATCGTAATA+AGG - chr6.4:39772063-39772082 MS.gene66586:CDS 35.0%
! TTAGGTCAAGTGATGATGAA+TGG - chr6.4:39771672-39771691 MS.gene66586:CDS 35.0%
!! GTGAAGGATTTTCAAGAAGA+TGG - chr6.4:39772124-39772143 MS.gene66586:CDS 35.0%
!! TGGAAGCATTGAGTTTGATT+AGG - chr6.4:39771654-39771673 MS.gene66586:CDS 35.0%
AGATTAAGGATCATGAGTGG+AGG - chr6.4:39772014-39772033 MS.gene66586:CDS 40.0%
AGGACTACGTGTAGTTCAAA+AGG - chr6.4:39771769-39771788 MS.gene66586:CDS 40.0%
CTATTGGTCCCACCAAAAAA+TGG + chr6.4:39771842-39771861 None:intergenic 40.0%
CTTGAGCCGTTGAAAATTCT+AGG - chr6.4:39771800-39771819 MS.gene66586:CDS 40.0%
GAGTATTCCAATCTAAGGGT+TGG - chr6.4:39771965-39771984 MS.gene66586:CDS 40.0%
GATTAAGGATCATGAGTGGA+GGG - chr6.4:39772015-39772034 MS.gene66586:CDS 40.0%
GCTACAATTGCATATCATGC+AGG - chr6.4:39771749-39771768 MS.gene66586:CDS 40.0%
GGTTAAATTGTTGGAGGAGA+AGG - chr6.4:39772090-39772109 MS.gene66586:CDS 40.0%
GGTTGATCGTAATAAGGAGT+TGG - chr6.4:39772069-39772088 MS.gene66586:CDS 40.0%
TAACTCTGACTCAGTCCTAT+TGG + chr6.4:39771858-39771877 None:intergenic 40.0%
! GGAGTTGGTTAAATTGTTGG+AGG - chr6.4:39772084-39772103 MS.gene66586:CDS 40.0%
! TATGAATGGTGGAGATCCAT+TGG - chr6.4:39772048-39772067 MS.gene66586:CDS 40.0%
!!! CATTTTTTGGTGGGACCAAT+AGG - chr6.4:39771840-39771859 MS.gene66586:CDS 40.0%
!!! TGCGTCAACCATTTTTTGGT+GGG - chr6.4:39771831-39771850 MS.gene66586:CDS 40.0%
!!! TTGCGTCAACCATTTTTTGG+TGG - chr6.4:39771830-39771849 MS.gene66586:CDS 40.0%
ACCATTCCCAACCCTTAGAT+TGG + chr6.4:39771975-39771994 None:intergenic 45.0%
ATAGCATCATCATATGCCGC+CGG + chr6.4:39771635-39771654 None:intergenic 45.0%
TCCAATCTAAGGGTTGGGAA+TGG - chr6.4:39771971-39771990 MS.gene66586:CDS 45.0%
TGGGAGTTAGCGTTACCAAT+TGG - chr6.4:39771926-39771945 MS.gene66586:CDS 45.0%
! GTGTTGGTGAGTATGAATGG+TGG - chr6.4:39772037-39772056 MS.gene66586:CDS 45.0%
!! AGGGTGTTGGTGAGTATGAA+TGG - chr6.4:39772034-39772053 MS.gene66586:CDS 45.0%
ATTGTTGGAGGAGAAGGGTG+TGG - chr6.4:39772096-39772115 MS.gene66586:CDS 50.0%
! GGCGGCATATGATGATGCTA+TGG - chr6.4:39771634-39771653 MS.gene66586:CDS 50.0%
! GAAGGGTGTGGAAGTGGTGA+AGG - chr6.4:39772108-39772127 MS.gene66586:CDS 55.0%
! GGATCATGAGTGGAGGGTGT+TGG - chr6.4:39772021-39772040 MS.gene66586:CDS 55.0%
CATCATATGCCGCCGGTAGC+CGG + chr6.4:39771628-39771647 None:intergenic 60.0%
CGAGGCGACAACTGCTTCGA+CGG + chr6.4:39771579-39771598 None:intergenic 60.0%
CGTCGAAGCAGTTGTCGCCT+CGG - chr6.4:39771577-39771596 MS.gene66586:CDS 60.0%
GGTCGATTATCGCCTCGCAC+CGG - chr6.4:39771598-39771617 MS.gene66586:CDS 60.0%
TGCGAGGCGATAATCGACCG+AGG + chr6.4:39771597-39771616 None:intergenic 60.0%
! GGAGGAGAAGGGTGTGGAAG+TGG - chr6.4:39772102-39772121 MS.gene66586:CDS 60.0%
ATCGCCTCGCACCGGAGCAC+CGG - chr6.4:39771606-39771625 MS.gene66586:CDS 70.0%
GTAGCCGGTGCTCCGGTGCG+AGG + chr6.4:39771613-39771632 None:intergenic 75.0%
!! ACCGGAGCACCGGCTACCGG+CGG - chr6.4:39771616-39771635 MS.gene66586:CDS 75.0%
!! CGCACCGGAGCACCGGCTAC+CGG - chr6.4:39771613-39771632 MS.gene66586:CDS 75.0%
! GCCGCCGGTAGCCGGTGCTC+CGG + chr6.4:39771620-39771639 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 39771566 39772198 39771566 ID=MS.gene66586
chr6.4 mRNA 39771566 39772198 39771566 ID=MS.gene66586.t1;Parent=MS.gene66586
chr6.4 exon 39771566 39772198 39771566 ID=MS.gene66586.t1.exon1;Parent=MS.gene66586.t1
chr6.4 CDS 39771566 39772198 39771566 ID=cds.MS.gene66586.t1;Parent=MS.gene66586.t1
Gene Sequence

>MS.gene66586

ATGGCGGACACCGTCGAAGCAGTTGTCGCCTCGGTCGATTATCGCCTCGCACCGGAGCACCGGCTACCGGCGGCATATGATGATGCTATGGAAGCATTGAGTTTGATTAGGTCAAGTGATGATGAATGGTTGACTAAATACGTTGACTTTTCAAAATGTTTTCTAATGGGAAATAGTGCTGGAGCTACAATTGCATATCATGCAGGACTACGTGTAGTTCAAAAGGTGAATGATCTTGAGCCGTTGAAAATTCTAGGGATGATATTGCGTCAACCATTTTTTGGTGGGACCAATAGGACTGAGTCAGAGTTAAGACTTGAGAATGATCCTGTTTTTCCTTTGTGTGTTAGTGATTTGATGTGGGAGTTAGCGTTACCAATTGGTGTTAAACGTGATCATGAGTATTCCAATCTAAGGGTTGGGAATGGTGTTGATGAAAAACTTGCTAAGATTAAGGATCATGAGTGGAGGGTGTTGGTGAGTATGAATGGTGGAGATCCATTGGTTGATCGTAATAAGGAGTTGGTTAAATTGTTGGAGGAGAAGGGTGTGGAAGTGGTGAAGGATTTTCAAGAAGATGGTTTTCATGGGGTTGAGTTTTTTGAGCCATCCAAAGCAAAGAATTTCATTGAG

Protein sequence

>MS.gene66586.t1

MADTVEAVVASVDYRLAPEHRLPAAYDDAMEALSLIRSSDDEWLTKYVDFSKCFLMGNSAGATIAYHAGLRVVQKVNDLEPLKILGMILRQPFFGGTNRTESELRLENDPVFPLCVSDLMWELALPIGVKRDHEYSNLRVGNGVDEKLAKIKDHEWRVLVSMNGGDPLVDRNKELVKLLEEKGVEVVKDFQEDGFHGVEFFEPSKAKNFIE