Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66966.t1 | KEH25608.1 | 96.4 | 393 | 14 | 0 | 1 | 393 | 1 | 393 | 3.70E-220 | 773.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66966.t1 | Q9FG87 | 62.7 | 389 | 139 | 3 | 3 | 385 | 133 | 521 | 5.0e-141 | 502.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66966.t1 | A0A072U924 | 96.4 | 393 | 14 | 0 | 1 | 393 | 1 | 393 | 2.6e-220 | 773.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene66966 | 0.821586 | 3.73E-53 | -1.69E-46 |
| MS.gene050563 | MS.gene66966 | 0.805471 | 1.31E-49 | -1.69E-46 |
| MS.gene050584 | MS.gene66966 | 0.802252 | 6.13E-49 | -1.69E-46 |
| MS.gene05157 | MS.gene66966 | 0.802332 | 5.90E-49 | -1.69E-46 |
| MS.gene052142 | MS.gene66966 | 0.832773 | 7.81E-56 | -1.69E-46 |
| MS.gene053383 | MS.gene66966 | 0.826875 | 2.13E-54 | -1.69E-46 |
| MS.gene05456 | MS.gene66966 | 0.820451 | 6.81E-53 | -1.69E-46 |
| MS.gene055359 | MS.gene66966 | 0.802616 | 5.16E-49 | -1.69E-46 |
| MS.gene059006 | MS.gene66966 | 0.804397 | 2.20E-49 | -1.69E-46 |
| MS.gene059768 | MS.gene66966 | 0.801496 | 8.76E-49 | -1.69E-46 |
| MS.gene060691 | MS.gene66966 | 0.804063 | 2.59E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66966.t1 | MTR_6g032965 | 96.438 | 393 | 14 | 0 | 1 | 393 | 1 | 393 | 0.0 | 794 |
| MS.gene66966.t1 | MTR_1g076430 | 64.416 | 385 | 137 | 0 | 1 | 385 | 123 | 507 | 0.0 | 531 |
| MS.gene66966.t1 | MTR_1g041085 | 65.241 | 374 | 130 | 0 | 12 | 385 | 140 | 513 | 0.0 | 524 |
| MS.gene66966.t1 | MTR_2g054940 | 61.558 | 385 | 148 | 0 | 1 | 385 | 122 | 506 | 0.0 | 515 |
| MS.gene66966.t1 | MTR_4g054415 | 58.656 | 387 | 159 | 1 | 1 | 386 | 41 | 427 | 5.43e-170 | 482 |
| MS.gene66966.t1 | MTR_4g054630 | 58.656 | 387 | 159 | 1 | 1 | 386 | 82 | 468 | 7.75e-170 | 483 |
| MS.gene66966.t1 | MTR_7g081695 | 59.580 | 381 | 142 | 1 | 1 | 381 | 122 | 490 | 1.99e-165 | 482 |
| MS.gene66966.t1 | MTR_6g074880 | 57.441 | 383 | 162 | 1 | 1 | 382 | 84 | 466 | 5.67e-165 | 471 |
| MS.gene66966.t1 | MTR_7g118170 | 57.841 | 389 | 158 | 3 | 2 | 387 | 146 | 531 | 2.72e-164 | 471 |
| MS.gene66966.t1 | MTR_4g113280 | 58.073 | 384 | 150 | 1 | 2 | 385 | 112 | 484 | 4.00e-164 | 469 |
| MS.gene66966.t1 | MTR_4g070270 | 56.658 | 383 | 165 | 1 | 2 | 384 | 121 | 502 | 9.70e-160 | 459 |
| MS.gene66966.t1 | MTR_1g110930 | 53.351 | 373 | 173 | 1 | 13 | 385 | 120 | 491 | 8.64e-150 | 433 |
| MS.gene66966.t1 | MTR_3g105550 | 51.145 | 393 | 182 | 2 | 2 | 384 | 144 | 536 | 7.84e-148 | 429 |
| MS.gene66966.t1 | MTR_5g056510 | 52.547 | 373 | 176 | 1 | 13 | 385 | 120 | 491 | 1.95e-146 | 424 |
| MS.gene66966.t1 | MTR_2g007020 | 52.186 | 366 | 173 | 2 | 22 | 386 | 89 | 453 | 3.16e-136 | 397 |
| MS.gene66966.t1 | MTR_7g118160 | 48.843 | 389 | 155 | 4 | 2 | 388 | 77 | 423 | 3.12e-129 | 378 |
| MS.gene66966.t1 | MTR_2g436480 | 47.532 | 385 | 199 | 2 | 2 | 384 | 68 | 451 | 2.43e-127 | 374 |
| MS.gene66966.t1 | MTR_2g013740 | 47.606 | 376 | 191 | 3 | 2 | 376 | 133 | 503 | 2.74e-121 | 361 |
| MS.gene66966.t1 | MTR_1g102290 | 40.314 | 382 | 227 | 1 | 2 | 382 | 103 | 484 | 1.34e-96 | 297 |
| MS.gene66966.t1 | MTR_4g054260 | 56.299 | 254 | 93 | 2 | 130 | 382 | 87 | 323 | 4.87e-95 | 287 |
| MS.gene66966.t1 | MTR_4g062190 | 39.756 | 410 | 217 | 7 | 2 | 392 | 55 | 453 | 5.40e-95 | 292 |
| MS.gene66966.t1 | MTR_4g062270 | 39.196 | 398 | 227 | 6 | 2 | 392 | 55 | 444 | 1.18e-92 | 286 |
| MS.gene66966.t1 | MTR_2g096210 | 40.665 | 391 | 201 | 7 | 12 | 381 | 64 | 444 | 3.92e-92 | 285 |
| MS.gene66966.t1 | MTR_1g115560 | 37.845 | 399 | 220 | 6 | 5 | 381 | 59 | 451 | 8.18e-92 | 285 |
| MS.gene66966.t1 | MTR_4g062170 | 38.670 | 406 | 226 | 6 | 2 | 392 | 55 | 452 | 2.87e-89 | 278 |
| MS.gene66966.t1 | MTR_6g051440 | 41.979 | 374 | 210 | 3 | 12 | 382 | 113 | 482 | 5.47e-88 | 275 |
| MS.gene66966.t1 | MTR_3g072980 | 40.642 | 374 | 215 | 3 | 12 | 382 | 114 | 483 | 7.68e-88 | 274 |
| MS.gene66966.t1 | MTR_3g072940 | 40.642 | 374 | 215 | 3 | 12 | 382 | 114 | 483 | 8.10e-88 | 274 |
| MS.gene66966.t1 | MTR_4g053905 | 60.870 | 161 | 63 | 0 | 226 | 386 | 1 | 161 | 3.52e-69 | 215 |
| MS.gene66966.t1 | MTR_4g054265 | 46.753 | 77 | 41 | 0 | 1 | 77 | 41 | 117 | 3.99e-16 | 74.3 |
| MS.gene66966.t1 | MTR_4g078730 | 22.692 | 260 | 164 | 7 | 97 | 351 | 152 | 379 | 8.90e-12 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66966.t1 | AT1G04220 | 61.381 | 391 | 144 | 3 | 2 | 385 | 126 | 516 | 0.0 | 515 |
| MS.gene66966.t1 | AT2G26640 | 61.818 | 385 | 147 | 0 | 1 | 385 | 120 | 504 | 3.73e-180 | 511 |
| MS.gene66966.t1 | AT5G43760 | 62.564 | 390 | 140 | 3 | 2 | 385 | 132 | 521 | 5.48e-177 | 503 |
| MS.gene66966.t1 | AT2G16280 | 57.963 | 383 | 160 | 1 | 2 | 384 | 129 | 510 | 1.97e-165 | 473 |
| MS.gene66966.t1 | AT1G01120 | 56.952 | 374 | 160 | 1 | 10 | 382 | 151 | 524 | 4.13e-165 | 473 |
| MS.gene66966.t1 | AT1G19440 | 56.919 | 383 | 164 | 1 | 2 | 384 | 133 | 514 | 9.56e-163 | 467 |
| MS.gene66966.t1 | AT4G34510 | 57.713 | 376 | 157 | 2 | 12 | 386 | 113 | 487 | 3.00e-161 | 462 |
| MS.gene66966.t1 | AT2G46720 | 53.264 | 383 | 177 | 1 | 2 | 382 | 81 | 463 | 1.24e-154 | 444 |
| MS.gene66966.t1 | AT4G34520 | 56.604 | 371 | 159 | 2 | 15 | 384 | 121 | 490 | 7.13e-150 | 434 |
| MS.gene66966.t1 | AT1G25450 | 54.424 | 373 | 169 | 1 | 13 | 385 | 116 | 487 | 2.72e-149 | 432 |
| MS.gene66966.t1 | AT4G34250 | 56.557 | 366 | 158 | 1 | 22 | 387 | 126 | 490 | 2.17e-148 | 429 |
| MS.gene66966.t1 | AT1G68530 | 54.521 | 365 | 163 | 2 | 22 | 385 | 130 | 492 | 9.67e-148 | 428 |
| MS.gene66966.t1 | AT2G15090 | 54.026 | 385 | 171 | 3 | 5 | 384 | 96 | 479 | 2.93e-147 | 426 |
| MS.gene66966.t1 | AT3G10280 | 51.958 | 383 | 175 | 2 | 2 | 382 | 81 | 456 | 6.57e-147 | 424 |
| MS.gene66966.t1 | AT2G26250 | 47.677 | 409 | 188 | 1 | 2 | 384 | 142 | 550 | 1.92e-140 | 411 |
| MS.gene66966.t1 | AT1G71160 | 48.794 | 373 | 189 | 2 | 10 | 382 | 76 | 446 | 2.08e-128 | 377 |
| MS.gene66966.t1 | AT5G49070 | 47.340 | 376 | 195 | 2 | 12 | 385 | 82 | 456 | 5.78e-121 | 358 |
| MS.gene66966.t1 | AT3G52160 | 45.604 | 364 | 167 | 2 | 10 | 373 | 4 | 336 | 3.71e-111 | 329 |
| MS.gene66966.t1 | AT3G52160 | 44.892 | 372 | 174 | 2 | 2 | 373 | 109 | 449 | 2.08e-109 | 328 |
| MS.gene66966.t1 | AT2G28630 | 39.163 | 406 | 229 | 6 | 2 | 392 | 55 | 457 | 7.02e-97 | 297 |
| MS.gene66966.t1 | AT2G28630 | 39.163 | 406 | 229 | 6 | 2 | 392 | 55 | 457 | 7.02e-97 | 297 |
| MS.gene66966.t1 | AT5G04530 | 41.860 | 387 | 212 | 6 | 2 | 381 | 57 | 437 | 2.27e-96 | 296 |
| MS.gene66966.t1 | AT1G07720 | 39.695 | 393 | 218 | 6 | 5 | 381 | 58 | 447 | 1.58e-93 | 289 |
| MS.gene66966.t1 | AT1G07720 | 39.695 | 393 | 218 | 6 | 5 | 381 | 101 | 490 | 3.02e-93 | 289 |
| MS.gene66966.t1 | AT1G68530 | 53.182 | 220 | 103 | 0 | 22 | 241 | 130 | 349 | 2.18e-80 | 251 |
| MS.gene66966.t1 | AT5G49070 | 43.966 | 232 | 130 | 0 | 12 | 243 | 82 | 313 | 3.42e-61 | 200 |
| MS.gene66966.t1 | AT4G34850 | 25.229 | 218 | 131 | 6 | 115 | 329 | 91 | 279 | 4.05e-12 | 67.0 |
| MS.gene66966.t1 | AT4G34850 | 25.229 | 218 | 131 | 6 | 115 | 329 | 164 | 352 | 8.38e-12 | 66.6 |
Find 61 sgRNAs with CRISPR-Local
Find 149 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATGTTCCTGAAGCATTAT+TGG | 0.209167 | 6.4:+4149956 | MS.gene66966:CDS |
| AAAATATGGCAAATGGCTTT+TGG | 0.246325 | 6.4:+4152820 | MS.gene66966:CDS |
| GTTAATCATTATAAACTTAG+AGG | 0.259701 | 6.4:+4150149 | MS.gene66966:CDS |
| TTGGATAGAGCAAAACATTT+TGG | 0.264127 | 6.4:+4149864 | MS.gene66966:CDS |
| AGCACTAAAGCATATGAGTT+TGG | 0.291741 | 6.4:-4152202 | None:intergenic |
| TCCATTAGTCCTCCCAATTT+CGG | 0.294314 | 6.4:+4152522 | MS.gene66966:CDS |
| TCCGAAATTGGGAGGACTAA+TGG | 0.324601 | 6.4:-4152523 | None:intergenic |
| AGCTAAGGGGAGAGTTAAAA+AGG | 0.330815 | 6.4:+4152792 | MS.gene66966:CDS |
| AAAGTTGAAGCTAAAGATAT+TGG | 0.336152 | 6.4:+4150068 | MS.gene66966:CDS |
| TTGTTCATTGGCAATGGAAC+AGG | 0.342492 | 6.4:-4152940 | None:intergenic |
| AACTTAAAATTCGGCATGTA+TGG | 0.357301 | 6.4:-4152604 | None:intergenic |
| TTAATCATATGTTTGTTCAT+TGG | 0.363214 | 6.4:-4152952 | None:intergenic |
| TTGCACAAAAGAAATGTTGT+TGG | 0.390520 | 6.4:+4149845 | None:intergenic |
| GATCTATAATCTTAGTGGTA+TGG | 0.390916 | 6.4:+4150181 | MS.gene66966:CDS |
| GCACTAAAGCATATGAGTTT+GGG | 0.397910 | 6.4:-4152201 | None:intergenic |
| TACTAATTGTCTCTTTCGAA+TGG | 0.399551 | 6.4:+4152282 | MS.gene66966:CDS |
| GGAACTTCCAATAATGCTTC+AGG | 0.401725 | 6.4:-4149963 | None:intergenic |
| AATATCTTTAGCTTCAACTT+TGG | 0.416100 | 6.4:-4150066 | None:intergenic |
| TCGAACGCTAACTTAAAATT+CGG | 0.417831 | 6.4:-4152613 | None:intergenic |
| TTAAGGTTAATACTGCTGTT+TGG | 0.420779 | 6.4:+4152851 | MS.gene66966:CDS |
| CTATATAGTGTCATTCTTGA+AGG | 0.424575 | 6.4:-4152718 | None:intergenic |
| ATCTATAATCTTAGTGGTAT+GGG | 0.428495 | 6.4:+4150182 | MS.gene66966:CDS |
| CGATGGATGACTTGAGAGTA+GGG | 0.457246 | 6.4:-4152317 | None:intergenic |
| GGGACATAAAATATGGCAAA+TGG | 0.457927 | 6.4:+4152813 | MS.gene66966:CDS |
| ACTAATTGTCTCTTTCGAAT+GGG | 0.459375 | 6.4:+4152283 | MS.gene66966:CDS |
| TCATCAAGTGTTAGTCTTGG+TGG | 0.459635 | 6.4:-4149984 | None:intergenic |
| AAATACCTCAAGAAGACGTT+TGG | 0.460466 | 6.4:-4150237 | None:intergenic |
| TGCTTCAGGAACATAAGTGC+TGG | 0.461711 | 6.4:-4149949 | None:intergenic |
| ACACATCATCAAGTTCTGTT+TGG | 0.465892 | 6.4:+4152749 | MS.gene66966:CDS |
| AGAATGACACTATATAGATA+TGG | 0.470646 | 6.4:+4152724 | MS.gene66966:CDS |
| AGTGGTATGGGATGCAGTGC+TGG | 0.470681 | 6.4:+4150194 | MS.gene66966:CDS |
| ATATGTTTGTTCATTGGCAA+TGG | 0.471516 | 6.4:-4152946 | None:intergenic |
| GCTTCATCAAGTGTTAGTCT+TGG | 0.477201 | 6.4:-4149987 | None:intergenic |
| TTCGAAAGAGACAATTAGTA+AGG | 0.478311 | 6.4:-4152279 | None:intergenic |
| ATTGTCTTATTGTGAAGCTA+AGG | 0.483820 | 6.4:+4152777 | MS.gene66966:CDS |
| CCGATGGATGACTTGAGAGT+AGG | 0.518403 | 6.4:-4152318 | None:intergenic |
| TTTGAGCGGCTACGGTCCGA+TGG | 0.522197 | 6.4:-4152334 | None:intergenic |
| TTGTCTTATTGTGAAGCTAA+GGG | 0.526933 | 6.4:+4152778 | MS.gene66966:CDS |
| CAATTAGTAAGGATCATTGA+AGG | 0.529851 | 6.4:-4152268 | None:intergenic |
| TTAATCATTATAAACTTAGA+GGG | 0.529909 | 6.4:+4150150 | MS.gene66966:CDS |
| AGGATCTCATGAATGTTGCT+AGG | 0.531519 | 6.4:+4152467 | MS.gene66966:CDS |
| GCGGTAGAGCGGTTCTTGAT+CGG | 0.540567 | 6.4:+4152656 | MS.gene66966:CDS |
| AATTGTCTCTTTCGAATGGG+TGG | 0.541404 | 6.4:+4152286 | MS.gene66966:CDS |
| TAAAGATATTGGAATTCTTG+TGG | 0.557701 | 6.4:+4150079 | MS.gene66966:CDS |
| GGATCTCATGAATGTTGCTA+GGG | 0.577726 | 6.4:+4152468 | MS.gene66966:CDS |
| TCTCTTTCGAATGGGTGGAT+CGG | 0.578474 | 6.4:+4152291 | MS.gene66966:CDS |
| AATTAGTAAGGATCATTGAA+GGG | 0.585870 | 6.4:-4152267 | None:intergenic |
| CAGTGCTGGTGTTATTGCAG+TGG | 0.587920 | 6.4:+4150208 | MS.gene66966:CDS |
| TGTTCATTGGCAATGGAACA+GGG | 0.590457 | 6.4:-4152939 | None:intergenic |
| CTTTGCCAAACGTCTTCTTG+AGG | 0.595847 | 6.4:+4150232 | MS.gene66966:CDS |
| CATGCATACGGGCGGTAGAG+CGG | 0.599254 | 6.4:+4152645 | MS.gene66966:CDS |
| ACAATAGTATCTGAAAGTGA+TGG | 0.605880 | 6.4:-4150128 | None:intergenic |
| GTATCTGAAAGTGATGGTGT+AGG | 0.606742 | 6.4:-4150122 | None:intergenic |
| GCTTCAGGAACATAAGTGCT+GGG | 0.608992 | 6.4:-4149948 | None:intergenic |
| ACTCGGTGTTAAACTATCGA+AGG | 0.617292 | 6.4:+4152447 | MS.gene66966:CDS |
| TGTCTTATTGTGAAGCTAAG+GGG | 0.629341 | 6.4:+4152779 | MS.gene66966:CDS |
| CGTACACACATAACATCGCT+CGG | 0.629342 | 6.4:+4152499 | MS.gene66966:CDS |
| CCTACTCTCAAGTCATCCAT+CGG | 0.633387 | 6.4:+4152318 | MS.gene66966:CDS |
| GCAATTGTAACTGTTGTCAT+CGG | 0.639808 | 6.4:-4152395 | None:intergenic |
| CAAATCTCAAGCATATACAA+AGG | 0.644919 | 6.4:+4152238 | MS.gene66966:CDS |
| CATTCGTTGCGCACTCACGT+AGG | 0.677333 | 6.4:+4152370 | MS.gene66966:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAAATTATAAAAGAGT+GGG | + | chr6.4:4150854-4150873 | MS.gene66966:intron | 10.0% |
| !! | TTAAAAAAATTATAAAAGAG+TGG | + | chr6.4:4150853-4150872 | MS.gene66966:intron | 10.0% |
| !!! | ATTATTTTTGTTTTTTTTGT+TGG | - | chr6.4:4151814-4151833 | None:intergenic | 10.0% |
| !!! | GTATATGTTATTTTTTTTTT+CGG | - | chr6.4:4151407-4151426 | None:intergenic | 10.0% |
| !!! | TATTTTTCTTTTTCTTTTTT+TGG | - | chr6.4:4151230-4151249 | None:intergenic | 10.0% |
| !! | AAAAAAAAAATGAAAATGTC+TGG | - | chr6.4:4150474-4150493 | None:intergenic | 15.0% |
| !! | TTAATCATTATAAACTTAGA+GGG | + | chr6.4:4150150-4150169 | MS.gene66966:CDS | 15.0% |
| !! | TTATATGAGAAAACAATATA+TGG | - | chr6.4:4151647-4151666 | None:intergenic | 15.0% |
| !!! | AAAGAGTACAAAATTATTTA+AGG | + | chr6.4:4150324-4150343 | MS.gene66966:intron | 15.0% |
| !!! | ATATTGTTTTCTCATATAAT+TGG | + | chr6.4:4151648-4151667 | MS.gene66966:intron | 15.0% |
| !!! | GTTTGTTAAATTTATTTGTA+GGG | - | chr6.4:4152095-4152114 | None:intergenic | 15.0% |
| !!! | TATTTTATCTAAAAGGTTAT+TGG | + | chr6.4:4150705-4150724 | MS.gene66966:intron | 15.0% |
| !!! | TGAAGATATTTTATAATTGA+TGG | + | chr6.4:4151941-4151960 | MS.gene66966:intron | 15.0% |
| !!! | TGTTTGTTAAATTTATTTGT+AGG | - | chr6.4:4152096-4152115 | None:intergenic | 15.0% |
| !! | AAAATAAAATATGCAAGATC+CGG | + | chr6.4:4151361-4151380 | MS.gene66966:intron | 20.0% |
| !! | AACTATATGAAATTGATGAA+TGG | - | chr6.4:4150767-4150786 | None:intergenic | 20.0% |
| !! | ACCTCTAGTATTAAAATATT+TGG | - | chr6.4:4150523-4150542 | None:intergenic | 20.0% |
| !! | ATAATCAAGCTTAATTAGTT+AGG | - | chr6.4:4152157-4152176 | None:intergenic | 20.0% |
| !! | CAGAATCTTTAATTCAATAA+TGG | - | chr6.4:4151756-4151775 | None:intergenic | 20.0% |
| !! | CCTCTAGTATTAAAATATTT+GGG | - | chr6.4:4150522-4150541 | None:intergenic | 20.0% |
| !! | GAGATATCAATATGTATTTA+TGG | + | chr6.4:4150818-4150837 | MS.gene66966:intron | 20.0% |
| !! | GTTAATCATTATAAACTTAG+AGG | + | chr6.4:4150149-4150168 | MS.gene66966:CDS | 20.0% |
| !! | GTTATTTACATATAGCAAAA+GGG | + | chr6.4:4150959-4150978 | MS.gene66966:intron | 20.0% |
| !! | TTAAAAAGGGACATAAAATA+TGG | + | chr6.4:4152806-4152825 | MS.gene66966:CDS | 20.0% |
| !! | TTAAAAATGTTCTCATATAG+TGG | - | chr6.4:4151608-4151627 | None:intergenic | 20.0% |
| !! | TTAATCATATGTTTGTTCAT+TGG | - | chr6.4:4152955-4152974 | None:intergenic | 20.0% |
| !!! | AAGAGTTTTGCTATTTATTA+TGG | + | chr6.4:4151472-4151491 | MS.gene66966:intron | 20.0% |
| !!! | ACAAATAAAAAAAGTGTAGT+TGG | + | chr6.4:4151097-4151116 | MS.gene66966:intron | 20.0% |
| !!! | ACTATTTTATACTTGTTTGT+TGG | + | chr6.4:4150385-4150404 | MS.gene66966:intron | 20.0% |
| !!! | ATTTTGATCTATAATCTTAG+TGG | + | chr6.4:4150176-4150195 | MS.gene66966:CDS | 20.0% |
| !!! | GAATCAAATTTAGTTCTTTT+TGG | + | chr6.4:4150017-4150036 | MS.gene66966:CDS | 20.0% |
| !!! | TGATTCATAAGATTTTAGAA+AGG | + | chr6.4:4149913-4149932 | MS.gene66966:CDS | 20.0% |
| ! | AAAGTTGAAGCTAAAGATAT+TGG | + | chr6.4:4150068-4150087 | MS.gene66966:CDS | 25.0% |
| ! | AAATAAAATATGCAAGATCC+GGG | + | chr6.4:4151362-4151381 | MS.gene66966:intron | 25.0% |
| ! | AATTAGTAAGGATCATTGAA+GGG | - | chr6.4:4152270-4152289 | None:intergenic | 25.0% |
| ! | AGAATGACACTATATAGATA+TGG | + | chr6.4:4152724-4152743 | MS.gene66966:CDS | 25.0% |
| ! | ATCTATAATCTTAGTGGTAT+GGG | + | chr6.4:4150182-4150201 | MS.gene66966:CDS | 25.0% |
| ! | ATGTGAAAAATTTAGTTGAG+AGG | + | chr6.4:4152557-4152576 | MS.gene66966:CDS | 25.0% |
| ! | CTAAATCATTGAAAAATGCT+TGG | + | chr6.4:4152896-4152915 | MS.gene66966:CDS | 25.0% |
| ! | GGTTATTTACATATAGCAAA+AGG | + | chr6.4:4150958-4150977 | MS.gene66966:intron | 25.0% |
| ! | TAAAGATATTGGAATTCTTG+TGG | + | chr6.4:4150079-4150098 | MS.gene66966:CDS | 25.0% |
| ! | TATTTATGACATGCAATTAC+AGG | + | chr6.4:4152174-4152193 | MS.gene66966:intron | 25.0% |
| !! | AATATCTTTAGCTTCAACTT+TGG | - | chr6.4:4150069-4150088 | None:intergenic | 25.0% |
| !! | CATTAGCTGTATTAATTTGA+AGG | + | chr6.4:4151139-4151158 | MS.gene66966:intron | 25.0% |
| !!! | ATACTGGAAGATACTTTTTT+TGG | - | chr6.4:4151675-4151694 | None:intergenic | 25.0% |
| !!! | CAAGCATTTTTCAATGATTT+AGG | - | chr6.4:4152898-4152917 | None:intergenic | 25.0% |
| !!! | CCCAAATATTTTAATACTAG+AGG | + | chr6.4:4150519-4150538 | MS.gene66966:intron | 25.0% |
| !!! | CGGTTTTAGTTGTTTTTTTT+TGG | + | chr6.4:4150937-4150956 | MS.gene66966:intron | 25.0% |
| !!! | GTTATTTTTTTTTTCGGTAG+TGG | - | chr6.4:4151401-4151420 | None:intergenic | 25.0% |
| AAAATATTTGGGTCTCTCTA+TGG | - | chr6.4:4150511-4150530 | None:intergenic | 30.0% | |
| AACTTAAAATTCGGCATGTA+TGG | - | chr6.4:4152607-4152626 | None:intergenic | 30.0% | |
| AATAAAATATGCAAGATCCG+GGG | + | chr6.4:4151363-4151382 | MS.gene66966:intron | 30.0% | |
| ACAATAGTATCTGAAAGTGA+TGG | - | chr6.4:4150131-4150150 | None:intergenic | 30.0% | |
| ACAATGTCGATATGTTCATA+CGG | + | chr6.4:4151707-4151726 | MS.gene66966:intron | 30.0% | |
| ACTAATTGTCTCTTTCGAAT+GGG | + | chr6.4:4152283-4152302 | MS.gene66966:CDS | 30.0% | |
| ATATGTTTGTTCATTGGCAA+TGG | - | chr6.4:4152949-4152968 | None:intergenic | 30.0% | |
| ATCATTGAAAAATGCTTGGA+TGG | + | chr6.4:4152900-4152919 | MS.gene66966:CDS | 30.0% | |
| CAAATCTCAAGCATATACAA+AGG | + | chr6.4:4152238-4152257 | MS.gene66966:CDS | 30.0% | |
| CAATTAGTAAGGATCATTGA+AGG | - | chr6.4:4152271-4152290 | None:intergenic | 30.0% | |
| GAAGACGAAAAAGAAATACT+CGG | + | chr6.4:4152430-4152449 | MS.gene66966:CDS | 30.0% | |
| GATCTATAATCTTAGTGGTA+TGG | + | chr6.4:4150181-4150200 | MS.gene66966:CDS | 30.0% | |
| GTGTGAATTTGTATCATTTG+CGG | - | chr6.4:4151538-4151557 | None:intergenic | 30.0% | |
| TACTAATTGTCTCTTTCGAA+TGG | + | chr6.4:4152282-4152301 | MS.gene66966:CDS | 30.0% | |
| TCGAACGCTAACTTAAAATT+CGG | - | chr6.4:4152616-4152635 | None:intergenic | 30.0% | |
| TTAAGGTTAATACTGCTGTT+TGG | + | chr6.4:4152851-4152870 | MS.gene66966:CDS | 30.0% | |
| TTCGAAAGAGACAATTAGTA+AGG | - | chr6.4:4152282-4152301 | None:intergenic | 30.0% | |
| ! | AAAATATGGCAAATGGCTTT+TGG | + | chr6.4:4152820-4152839 | MS.gene66966:CDS | 30.0% |
| ! | ACTTGAGTTTTTCCGAAATT+GGG | - | chr6.4:4152537-4152556 | None:intergenic | 30.0% |
| ! | ATTGTCTTATTGTGAAGCTA+AGG | + | chr6.4:4152777-4152796 | MS.gene66966:CDS | 30.0% |
| ! | CTATATAGTGTCATTCTTGA+AGG | - | chr6.4:4152721-4152740 | None:intergenic | 30.0% |
| ! | TACTTGAGTTTTTCCGAAAT+TGG | - | chr6.4:4152538-4152557 | None:intergenic | 30.0% |
| ! | TGATATTGCTTCTTGTTTGT+CGG | + | chr6.4:4150361-4150380 | MS.gene66966:intron | 30.0% |
| ! | TTATGTTCCTGAAGCATTAT+TGG | + | chr6.4:4149956-4149975 | MS.gene66966:CDS | 30.0% |
| ! | TTGGATAGAGCAAAACATTT+TGG | + | chr6.4:4149864-4149883 | MS.gene66966:CDS | 30.0% |
| ! | TTGTCTTATTGTGAAGCTAA+GGG | + | chr6.4:4152778-4152797 | MS.gene66966:CDS | 30.0% |
| !! | CATAAGATTTTAGAAAGGTC+AGG | + | chr6.4:4149918-4149937 | MS.gene66966:CDS | 30.0% |
| !! | TGAGCTTGATGATTTTCATA+TGG | + | chr6.4:4152693-4152712 | MS.gene66966:CDS | 30.0% |
| AAATACCTCAAGAAGACGTT+TGG | - | chr6.4:4150240-4150259 | None:intergenic | 35.0% | |
| ACACATCATCAAGTTCTGTT+TGG | + | chr6.4:4152749-4152768 | MS.gene66966:CDS | 35.0% | |
| AGCACTAAAGCATATGAGTT+TGG | - | chr6.4:4152205-4152224 | None:intergenic | 35.0% | |
| CAAATTACATGTAGCTAGCA+AGG | - | chr6.4:4152132-4152151 | None:intergenic | 35.0% | |
| GCAATTGTAACTGTTGTCAT+CGG | - | chr6.4:4152398-4152417 | None:intergenic | 35.0% | |
| GCACTAAAGCATATGAGTTT+GGG | - | chr6.4:4152204-4152223 | None:intergenic | 35.0% | |
| GGGACATAAAATATGGCAAA+TGG | + | chr6.4:4152813-4152832 | MS.gene66966:CDS | 35.0% | |
| GTTTCACTGTCAATCATCAT+AGG | + | chr6.4:4151729-4151748 | MS.gene66966:intron | 35.0% | |
| TAGTGGTGAAATAGATACAG+TGG | - | chr6.4:4151591-4151610 | None:intergenic | 35.0% | |
| TGTGCTAAGCTCACTTATTA+AGG | - | chr6.4:4152069-4152088 | None:intergenic | 35.0% | |
| TTGTGACTGAACACATATAC+TGG | - | chr6.4:4151691-4151710 | None:intergenic | 35.0% | |
| ! | CGATCAATTTTGCATGCATA+CGG | + | chr6.4:4152633-4152652 | MS.gene66966:CDS | 35.0% |
| ! | GATCAATTTTGCATGCATAC+GGG | + | chr6.4:4152634-4152653 | MS.gene66966:CDS | 35.0% |
| ! | TGAGTTTAGCTTAGTTGGTT+GGG | + | chr6.4:4151329-4151348 | MS.gene66966:intron | 35.0% |
| ! | TGTCTTATTGTGAAGCTAAG+GGG | + | chr6.4:4152779-4152798 | MS.gene66966:CDS | 35.0% |
| !! | ATTTTAGAAAGGTCAGGTGT+GGG | + | chr6.4:4149924-4149943 | MS.gene66966:CDS | 35.0% |
| !! | GCATCAACTGATATTTTGAG+CGG | - | chr6.4:4152351-4152370 | None:intergenic | 35.0% |
| !!! | TTTTTTTTTTCGGTAGTGGC+CGG | - | chr6.4:4151397-4151416 | None:intergenic | 35.0% |
| AATTGTCTCTTTCGAATGGG+TGG | + | chr6.4:4152286-4152305 | MS.gene66966:CDS | 40.0% | |
| ACCTTAGTCCTACTCAGATA+CGG | - | chr6.4:4150913-4150932 | None:intergenic | 40.0% | |
| AGCTAAGGGGAGAGTTAAAA+AGG | + | chr6.4:4152792-4152811 | MS.gene66966:CDS | 40.0% | |
| AGGATCTCATGAATGTTGCT+AGG | + | chr6.4:4152467-4152486 | MS.gene66966:CDS | 40.0% | |
| GCTAAGGGGAGAGTTAAAAA+GGG | + | chr6.4:4152793-4152812 | MS.gene66966:CDS | 40.0% | |
| GGAACTTCCAATAATGCTTC+AGG | - | chr6.4:4149966-4149985 | None:intergenic | 40.0% | |
| GGATCTCATGAATGTTGCTA+GGG | + | chr6.4:4152468-4152487 | MS.gene66966:CDS | 40.0% | |
| TCCATTAGTCCTCCCAATTT+CGG | + | chr6.4:4152522-4152541 | MS.gene66966:CDS | 40.0% | |
| TGAGTAGGACTAAGGTAGTT+CGG | + | chr6.4:4150917-4150936 | MS.gene66966:intron | 40.0% | |
| TGTTCATTGGCAATGGAACA+GGG | - | chr6.4:4152942-4152961 | None:intergenic | 40.0% | |
| TTGTTCATTGGCAATGGAAC+AGG | - | chr6.4:4152943-4152962 | None:intergenic | 40.0% | |
| ! | GCTTCATCAAGTGTTAGTCT+TGG | - | chr6.4:4149990-4150009 | None:intergenic | 40.0% |
| ! | GGCTTTTGGATCTGGATTTA+AGG | + | chr6.4:4152834-4152853 | MS.gene66966:CDS | 40.0% |
| ! | GTATCTGAAAGTGATGGTGT+AGG | - | chr6.4:4150125-4150144 | None:intergenic | 40.0% |
| ! | GTGAGTTTAGCTTAGTTGGT+TGG | + | chr6.4:4151328-4151347 | MS.gene66966:intron | 40.0% |
| ! | TGAGTTTTTCCGAAATTGGG+AGG | - | chr6.4:4152534-4152553 | None:intergenic | 40.0% |
| !! | ACTCGGTGTTAAACTATCGA+AGG | + | chr6.4:4152447-4152466 | MS.gene66966:CDS | 40.0% |
| !! | ACTGATATTTTGAGCGGCTA+CGG | - | chr6.4:4152345-4152364 | None:intergenic | 40.0% |
| !! | GATTTTAGAAAGGTCAGGTG+TGG | + | chr6.4:4149923-4149942 | MS.gene66966:CDS | 40.0% |
| !! | TCATCAAGTGTTAGTCTTGG+TGG | - | chr6.4:4149987-4150006 | None:intergenic | 40.0% |
| !!! | TTTTTTTTTCGGTAGTGGCC+GGG | - | chr6.4:4151396-4151415 | None:intergenic | 40.0% |
| CCAACTAAGCTAAACTCACG+AGG | - | chr6.4:4151327-4151346 | None:intergenic | 45.0% | |
| CCTACTCTCAAGTCATCCAT+CGG | + | chr6.4:4152318-4152337 | MS.gene66966:CDS | 45.0% | |
| CCTTAGTCCTACTCAGATAC+GGG | - | chr6.4:4150912-4150931 | None:intergenic | 45.0% | |
| CGATGGATGACTTGAGAGTA+GGG | - | chr6.4:4152320-4152339 | None:intergenic | 45.0% | |
| CGTACACACATAACATCGCT+CGG | + | chr6.4:4152499-4152518 | MS.gene66966:CDS | 45.0% | |
| CTTTGCCAAACGTCTTCTTG+AGG | + | chr6.4:4150232-4150251 | MS.gene66966:CDS | 45.0% | |
| GCTTCAGGAACATAAGTGCT+GGG | - | chr6.4:4149951-4149970 | None:intergenic | 45.0% | |
| TCCGAAATTGGGAGGACTAA+TGG | - | chr6.4:4152526-4152545 | None:intergenic | 45.0% | |
| TCTCTTTCGAATGGGTGGAT+CGG | + | chr6.4:4152291-4152310 | MS.gene66966:CDS | 45.0% | |
| TGCTTCAGGAACATAAGTGC+TGG | - | chr6.4:4149952-4149971 | None:intergenic | 45.0% | |
| ! | CAATTTTGCATGCATACGGG+CGG | + | chr6.4:4152637-4152656 | MS.gene66966:CDS | 45.0% |
| ! | CCTCGTGAGTTTAGCTTAGT+TGG | + | chr6.4:4151324-4151343 | MS.gene66966:intron | 45.0% |
| ! | TGGCAAATGGCTTTTGGATC+TGG | + | chr6.4:4152826-4152845 | MS.gene66966:CDS | 45.0% |
| !!! | TTTTTTTTCGGTAGTGGCCG+GGG | - | chr6.4:4151395-4151414 | None:intergenic | 45.0% |
| !!! | ATATTTTTATTTTATCTAAA+AGG | + | chr6.4:4150698-4150717 | MS.gene66966:intron | 5.0% |
| !!! | TTTTTTATTTGTATAAATTT+GGG | - | chr6.4:4151089-4151108 | None:intergenic | 5.0% |
| !!! | TTTTTTTATTTGTATAAATT+TGG | - | chr6.4:4151090-4151109 | None:intergenic | 5.0% |
| ACCCTCAAGCTCCAACCTAT+AGG | + | chr6.4:4150886-4150905 | MS.gene66966:intron | 50.0% | |
| CCCGTATCTGAGTAGGACTA+AGG | + | chr6.4:4150909-4150928 | MS.gene66966:intron | 50.0% | |
| CCGATGGATGACTTGAGAGT+AGG | - | chr6.4:4152321-4152340 | None:intergenic | 50.0% | |
| CTACTCAGATACGGGCCTAT+AGG | - | chr6.4:4150904-4150923 | None:intergenic | 50.0% | |
| CTATAGGCCCGTATCTGAGT+AGG | + | chr6.4:4150902-4150921 | MS.gene66966:intron | 50.0% | |
| TCAGATACGGGCCTATAGGT+TGG | - | chr6.4:4150900-4150919 | None:intergenic | 50.0% | |
| ! | GCCTATAGGTTGGAGCTTGA+GGG | - | chr6.4:4150890-4150909 | None:intergenic | 50.0% |
| !! | CAGTGCTGGTGTTATTGCAG+TGG | + | chr6.4:4150208-4150227 | MS.gene66966:CDS | 50.0% |
| AAGATCCGGGGTTCAAACCC+CGG | + | chr6.4:4151375-4151394 | MS.gene66966:intron | 55.0% | |
| AGTGGTATGGGATGCAGTGC+TGG | + | chr6.4:4150194-4150213 | MS.gene66966:CDS | 55.0% | |
| CATTCGTTGCGCACTCACGT+AGG | + | chr6.4:4152370-4152389 | MS.gene66966:CDS | 55.0% | |
| GCGGTAGAGCGGTTCTTGAT+CGG | + | chr6.4:4152656-4152675 | MS.gene66966:CDS | 55.0% | |
| ! | GGCCTATAGGTTGGAGCTTG+AGG | - | chr6.4:4150891-4150910 | None:intergenic | 55.0% |
| CATGCATACGGGCGGTAGAG+CGG | + | chr6.4:4152645-4152664 | MS.gene66966:CDS | 60.0% | |
| TTTGAGCGGCTACGGTCCGA+TGG | - | chr6.4:4152337-4152356 | None:intergenic | 60.0% | |
| AGTGGCCGGGGTTTGAACCC+CGG | - | chr6.4:4151383-4151402 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 4149858 | 4152981 | 4149858 | ID=MS.gene66966 |
| chr6.4 | mRNA | 4149858 | 4152981 | 4149858 | ID=MS.gene66966.t1;Parent=MS.gene66966 |
| chr6.4 | exon | 4149858 | 4150253 | 4149858 | ID=MS.gene66966.t1.exon1;Parent=MS.gene66966.t1 |
| chr6.4 | CDS | 4149858 | 4150253 | 4149858 | ID=cds.MS.gene66966.t1;Parent=MS.gene66966.t1 |
| chr6.4 | exon | 4152196 | 4152981 | 4152196 | ID=MS.gene66966.t1.exon2;Parent=MS.gene66966.t1 |
| chr6.4 | CDS | 4152196 | 4152981 | 4152196 | ID=cds.MS.gene66966.t1;Parent=MS.gene66966.t1 |
| Gene Sequence |
| Protein sequence |
