Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67504.t1 | XP_003612257.2 | 94.2 | 154 | 8 | 1 | 1 | 154 | 1 | 153 | 3.30E-68 | 267.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67504.t1 | G7JYR8 | 94.2 | 154 | 8 | 1 | 1 | 154 | 1 | 153 | 2.4e-68 | 267.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050496 | MS.gene67504 | -0.805542 | 1.27E-49 | -1.69E-46 |
| MS.gene050521 | MS.gene67504 | 0.801398 | 9.18E-49 | -1.69E-46 |
| MS.gene053957 | MS.gene67504 | 0.806117 | 9.61E-50 | -1.69E-46 |
| MS.gene055942 | MS.gene67504 | 0.811531 | 6.68E-51 | -1.69E-46 |
| MS.gene058015 | MS.gene67504 | 0.822094 | 2.85E-53 | -1.69E-46 |
| MS.gene058431 | MS.gene67504 | 0.808373 | 3.20E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67504.t1 | MTR_5g023030 | 95.455 | 154 | 6 | 1 | 1 | 154 | 1 | 153 | 2.34e-104 | 295 |
| MS.gene67504.t1 | MTR_5g023030 | 95.455 | 154 | 6 | 1 | 1 | 154 | 1 | 153 | 5.19e-104 | 295 |
| MS.gene67504.t1 | MTR_8g074760 | 54.658 | 161 | 60 | 7 | 1 | 154 | 1 | 155 | 5.87e-36 | 122 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTCTGCTTTGGATTTCTA+AGG | 0.172324 | 5.4:+14797729 | MS.gene67504:CDS |
| TTAGGAGAAGTGGGTTCTTT+TGG | 0.237235 | 5.4:-14797610 | None:intergenic |
| TGCTTTCTTCTAGCTTCCTT+AGG | 0.309418 | 5.4:-14797565 | None:intergenic |
| TCACCCATGCACGAGACTTT+AGG | 0.317786 | 5.4:-14797628 | None:intergenic |
| AAATTGTTACATTCACCTTT+CGG | 0.403917 | 5.4:-14797884 | None:intergenic |
| AAGGACAAGAAGTTACATAA+AGG | 0.412042 | 5.4:+14797679 | MS.gene67504:CDS |
| GCTTTCTTCTAGCTTCCTTA+GGG | 0.417399 | 5.4:-14797564 | None:intergenic |
| AATGAAACCACATGTGTTGT+AGG | 0.443488 | 5.4:-14797538 | None:intergenic |
| AGGACAAGAAGTTACATAAA+GGG | 0.488699 | 5.4:+14797680 | MS.gene67504:CDS |
| TCTAAGGGAAGTTATGAAGC+AGG | 0.489058 | 5.4:+14797745 | MS.gene67504:CDS |
| TTCTCCTAAAGTCTCGTGCA+TGG | 0.496688 | 5.4:+14797624 | MS.gene67504:CDS |
| ATCGACAAGGACTAAGAGTT+GGG | 0.514111 | 5.4:-14797513 | None:intergenic |
| AAGAAATTTACCAGTGGGAG+AGG | 0.523630 | 5.4:+14797832 | MS.gene67504:CDS |
| GCACGAGACTTTAGGAGAAG+TGG | 0.524552 | 5.4:-14797620 | None:intergenic |
| TGTGGTTTCATTATTCCCTA+AGG | 0.534785 | 5.4:+14797549 | MS.gene67504:CDS |
| CAACCACCAGTGTCTTTAGC+GGG | 0.552346 | 5.4:+14797481 | MS.gene67504:CDS |
| AAGCTAGAAGAAAGCACAGA+AGG | 0.555212 | 5.4:+14797572 | MS.gene67504:CDS |
| TCATAGAATCCAACGTTGGA+GGG | 0.555841 | 5.4:-14797810 | None:intergenic |
| AGCTAGAAGAAAGCACAGAA+GGG | 0.558361 | 5.4:+14797573 | MS.gene67504:CDS |
| TTCATAGAATCCAACGTTGG+AGG | 0.559594 | 5.4:-14797811 | None:intergenic |
| GATCGACAAGGACTAAGAGT+TGG | 0.560227 | 5.4:-14797514 | None:intergenic |
| AGTGAATGTCCCTCCAACGT+TGG | 0.563858 | 5.4:+14797801 | MS.gene67504:CDS |
| CACGAGACTTTAGGAGAAGT+GGG | 0.567559 | 5.4:-14797619 | None:intergenic |
| TCTCCTAAAGTCTCGTGCAT+GGG | 0.572514 | 5.4:+14797625 | MS.gene67504:CDS |
| GTTCTGCTTTGGATTTCTAA+GGG | 0.577860 | 5.4:+14797730 | MS.gene67504:CDS |
| CTAAGGGAAGTTATGAAGCA+GGG | 0.583061 | 5.4:+14797746 | MS.gene67504:CDS |
| TGATATCCCGCTAAAGACAC+TGG | 0.590824 | 5.4:-14797487 | None:intergenic |
| ACAACCACCAGTGTCTTTAG+CGG | 0.593632 | 5.4:+14797480 | MS.gene67504:CDS |
| GAGACAAGGTGGGAAAGAGA+AGG | 0.596540 | 5.4:+14797768 | MS.gene67504:CDS |
| ACACAATGATCCTCTCCCAC+TGG | 0.608376 | 5.4:-14797842 | None:intergenic |
| AGTTATGAAGCAGGGAGACA+AGG | 0.608744 | 5.4:+14797754 | MS.gene67504:CDS |
| CTATGAAGAAATTTACCAGT+GGG | 0.611053 | 5.4:+14797827 | MS.gene67504:CDS |
| TTCTTCATAGAATCCAACGT+TGG | 0.612106 | 5.4:-14797814 | None:intergenic |
| TATGAAGCAGGGAGACAAGG+TGG | 0.615974 | 5.4:+14797757 | MS.gene67504:CDS |
| TTGATGCAACACTATCCGAA+AGG | 0.634236 | 5.4:+14797869 | MS.gene67504:CDS |
| GCTAGAAGAAAGCACAGAAG+GGG | 0.641612 | 5.4:+14797574 | MS.gene67504:CDS |
| ATGAAGCAGGGAGACAAGGT+GGG | 0.641635 | 5.4:+14797758 | MS.gene67504:CDS |
| TCGATCACCTACAACACATG+TGG | 0.650680 | 5.4:+14797531 | MS.gene67504:CDS |
| TCTATGAAGAAATTTACCAG+TGG | 0.673132 | 5.4:+14797826 | MS.gene67504:CDS |
| TATCCCGCTAAAGACACTGG+TGG | 0.724982 | 5.4:-14797484 | None:intergenic |
| TGTGTTGTAGGTGATCGACA+AGG | 0.755971 | 5.4:-14797526 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGGACAAGAAGTTACATAA+AGG | + | chr5.4:14797679-14797698 | MS.gene67504:CDS | 30.0% | |
| AGGACAAGAAGTTACATAAA+GGG | + | chr5.4:14797680-14797699 | MS.gene67504:CDS | 30.0% | |
| CAAGAAGAAGAAGAAAAAGA+AGG | + | chr5.4:14797660-14797679 | MS.gene67504:CDS | 30.0% | |
| CTATGAAGAAATTTACCAGT+GGG | + | chr5.4:14797827-14797846 | MS.gene67504:CDS | 30.0% | |
| TCTATGAAGAAATTTACCAG+TGG | + | chr5.4:14797826-14797845 | MS.gene67504:CDS | 30.0% | |
| AATGAAACCACATGTGTTGT+AGG | - | chr5.4:14797541-14797560 | None:intergenic | 35.0% | |
| CAAACAAAAACGTTCTGCTT+TGG | + | chr5.4:14797719-14797738 | MS.gene67504:CDS | 35.0% | |
| TGTGGTTTCATTATTCCCTA+AGG | + | chr5.4:14797549-14797568 | MS.gene67504:CDS | 35.0% | |
| TTCTTCATAGAATCCAACGT+TGG | - | chr5.4:14797817-14797836 | None:intergenic | 35.0% | |
| ! | CAAAGCAGAACGTTTTTGTT+TGG | - | chr5.4:14797721-14797740 | None:intergenic | 35.0% |
| ! | GTTCTGCTTTGGATTTCTAA+GGG | + | chr5.4:14797730-14797749 | MS.gene67504:CDS | 35.0% |
| AAGAAATTTACCAGTGGGAG+AGG | + | chr5.4:14797832-14797851 | MS.gene67504:CDS | 40.0% | |
| ATCGACAAGGACTAAGAGTT+GGG | - | chr5.4:14797516-14797535 | None:intergenic | 40.0% | |
| CTAAGGGAAGTTATGAAGCA+GGG | + | chr5.4:14797746-14797765 | MS.gene67504:CDS | 40.0% | |
| TCATAGAATCCAACGTTGGA+GGG | - | chr5.4:14797813-14797832 | None:intergenic | 40.0% | |
| TCTAAGGGAAGTTATGAAGC+AGG | + | chr5.4:14797745-14797764 | MS.gene67504:CDS | 40.0% | |
| TTCATAGAATCCAACGTTGG+AGG | - | chr5.4:14797814-14797833 | None:intergenic | 40.0% | |
| TTGATGCAACACTATCCGAA+AGG | + | chr5.4:14797869-14797888 | MS.gene67504:CDS | 40.0% | |
| ! | AAGCTAGAAGAAAGCACAGA+AGG | + | chr5.4:14797572-14797591 | MS.gene67504:CDS | 40.0% |
| ! | AGCTAGAAGAAAGCACAGAA+GGG | + | chr5.4:14797573-14797592 | MS.gene67504:CDS | 40.0% |
| ! | CGTTCTGCTTTGGATTTCTA+AGG | + | chr5.4:14797729-14797748 | MS.gene67504:CDS | 40.0% |
| !! | GCTTTCTTCTAGCTTCCTTA+GGG | - | chr5.4:14797567-14797586 | None:intergenic | 40.0% |
| !! | TGCTTTCTTCTAGCTTCCTT+AGG | - | chr5.4:14797568-14797587 | None:intergenic | 40.0% |
| !! | TTAGGAGAAGTGGGTTCTTT+TGG | - | chr5.4:14797613-14797632 | None:intergenic | 40.0% |
| ACAACCACCAGTGTCTTTAG+CGG | + | chr5.4:14797480-14797499 | MS.gene67504:CDS | 45.0% | |
| AGTTATGAAGCAGGGAGACA+AGG | + | chr5.4:14797754-14797773 | MS.gene67504:CDS | 45.0% | |
| GATCGACAAGGACTAAGAGT+TGG | - | chr5.4:14797517-14797536 | None:intergenic | 45.0% | |
| TCGATCACCTACAACACATG+TGG | + | chr5.4:14797531-14797550 | MS.gene67504:CDS | 45.0% | |
| TCTCCTAAAGTCTCGTGCAT+GGG | + | chr5.4:14797625-14797644 | MS.gene67504:CDS | 45.0% | |
| TGATATCCCGCTAAAGACAC+TGG | - | chr5.4:14797490-14797509 | None:intergenic | 45.0% | |
| TGTGTTGTAGGTGATCGACA+AGG | - | chr5.4:14797529-14797548 | None:intergenic | 45.0% | |
| TTCTCCTAAAGTCTCGTGCA+TGG | + | chr5.4:14797624-14797643 | MS.gene67504:CDS | 45.0% | |
| ! | CACGAGACTTTAGGAGAAGT+GGG | - | chr5.4:14797622-14797641 | None:intergenic | 45.0% |
| ! | GCTAGAAGAAAGCACAGAAG+GGG | + | chr5.4:14797574-14797593 | MS.gene67504:CDS | 45.0% |
| ACACAATGATCCTCTCCCAC+TGG | - | chr5.4:14797845-14797864 | None:intergenic | 50.0% | |
| AGTGAATGTCCCTCCAACGT+TGG | + | chr5.4:14797801-14797820 | MS.gene67504:CDS | 50.0% | |
| ATGAAGCAGGGAGACAAGGT+GGG | + | chr5.4:14797758-14797777 | MS.gene67504:CDS | 50.0% | |
| CAACCACCAGTGTCTTTAGC+GGG | + | chr5.4:14797481-14797500 | MS.gene67504:CDS | 50.0% | |
| GAGACAAGGTGGGAAAGAGA+AGG | + | chr5.4:14797768-14797787 | MS.gene67504:CDS | 50.0% | |
| TATCCCGCTAAAGACACTGG+TGG | - | chr5.4:14797487-14797506 | None:intergenic | 50.0% | |
| TATGAAGCAGGGAGACAAGG+TGG | + | chr5.4:14797757-14797776 | MS.gene67504:CDS | 50.0% | |
| TCACCCATGCACGAGACTTT+AGG | - | chr5.4:14797631-14797650 | None:intergenic | 50.0% | |
| ! | GCACGAGACTTTAGGAGAAG+TGG | - | chr5.4:14797623-14797642 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 14797430 | 14797894 | 14797430 | ID=MS.gene67504 |
| chr5.4 | mRNA | 14797430 | 14797894 | 14797430 | ID=MS.gene67504.t1;Parent=MS.gene67504 |
| chr5.4 | exon | 14797430 | 14797894 | 14797430 | ID=MS.gene67504.t1.exon1;Parent=MS.gene67504.t1 |
| chr5.4 | CDS | 14797430 | 14797894 | 14797430 | ID=cds.MS.gene67504.t1;Parent=MS.gene67504.t1 |
| Gene Sequence |
| Protein sequence |
