Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67684.t1 | XP_003612257.2 | 94.2 | 154 | 8 | 1 | 1 | 154 | 1 | 153 | 3.30E-68 | 267.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67684.t1 | G7JYR8 | 94.2 | 154 | 8 | 1 | 1 | 154 | 1 | 153 | 2.4e-68 | 267.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050496 | MS.gene67684 | -0.801678 | 8.04E-49 | -1.69E-46 |
| MS.gene050521 | MS.gene67684 | 0.807598 | 4.67E-50 | -1.69E-46 |
| MS.gene053957 | MS.gene67684 | 0.813114 | 3.01E-51 | -1.69E-46 |
| MS.gene055942 | MS.gene67684 | 0.812263 | 4.63E-51 | -1.69E-46 |
| MS.gene058015 | MS.gene67684 | 0.815159 | 1.07E-51 | -1.69E-46 |
| MS.gene058431 | MS.gene67684 | 0.814406 | 1.57E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67684.t1 | MTR_5g023030 | 95.455 | 154 | 6 | 1 | 1 | 154 | 1 | 153 | 2.34e-104 | 295 |
| MS.gene67684.t1 | MTR_5g023030 | 95.455 | 154 | 6 | 1 | 1 | 154 | 1 | 153 | 5.19e-104 | 295 |
| MS.gene67684.t1 | MTR_8g074760 | 54.658 | 161 | 60 | 7 | 1 | 154 | 1 | 155 | 5.87e-36 | 122 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTCTGCTTTGGATTTCTA+AGG | 0.172324 | 5.2:+13989354 | MS.gene67684:CDS |
| TTAGGAGAAGTGGGTTCTTT+TGG | 0.237235 | 5.2:-13989235 | None:intergenic |
| TGCTTTCTTCTAGCTTCCTT+AGG | 0.309418 | 5.2:-13989190 | None:intergenic |
| TCACCCATGCACGAGACTTT+AGG | 0.317786 | 5.2:-13989253 | None:intergenic |
| AAATTGTTACATTCACCTTT+CGG | 0.403917 | 5.2:-13989509 | None:intergenic |
| AAGGACAAGAAGTTACATAA+AGG | 0.412042 | 5.2:+13989304 | MS.gene67684:CDS |
| GCTTTCTTCTAGCTTCCTTA+GGG | 0.417399 | 5.2:-13989189 | None:intergenic |
| AATGAAACCACATGTGTTGT+AGG | 0.443488 | 5.2:-13989163 | None:intergenic |
| AGGACAAGAAGTTACATAAA+GGG | 0.488699 | 5.2:+13989305 | MS.gene67684:CDS |
| TCTAAGGGAAGTTATGAAGC+AGG | 0.489058 | 5.2:+13989370 | MS.gene67684:CDS |
| TTCTCCTAAAGTCTCGTGCA+TGG | 0.496688 | 5.2:+13989249 | MS.gene67684:CDS |
| ATCGACAAGGACTAAGAGTT+GGG | 0.514111 | 5.2:-13989138 | None:intergenic |
| AAGAAATTTACCAGTGGGAG+AGG | 0.523630 | 5.2:+13989457 | MS.gene67684:CDS |
| GCACGAGACTTTAGGAGAAG+TGG | 0.524552 | 5.2:-13989245 | None:intergenic |
| TGTGGTTTCATTATTCCCTA+AGG | 0.534785 | 5.2:+13989174 | MS.gene67684:CDS |
| CAACCACCAGTGTCTTTAGC+GGG | 0.552346 | 5.2:+13989106 | MS.gene67684:CDS |
| AAGCTAGAAGAAAGCACAGA+AGG | 0.555212 | 5.2:+13989197 | MS.gene67684:CDS |
| TCATAGAATCCAACGTTGGA+GGG | 0.555841 | 5.2:-13989435 | None:intergenic |
| AGCTAGAAGAAAGCACAGAA+GGG | 0.558361 | 5.2:+13989198 | MS.gene67684:CDS |
| TTCATAGAATCCAACGTTGG+AGG | 0.559594 | 5.2:-13989436 | None:intergenic |
| GATCGACAAGGACTAAGAGT+TGG | 0.560227 | 5.2:-13989139 | None:intergenic |
| AGTGAATGTCCCTCCAACGT+TGG | 0.563858 | 5.2:+13989426 | MS.gene67684:CDS |
| CACGAGACTTTAGGAGAAGT+GGG | 0.567559 | 5.2:-13989244 | None:intergenic |
| TCTCCTAAAGTCTCGTGCAT+GGG | 0.572514 | 5.2:+13989250 | MS.gene67684:CDS |
| GTTCTGCTTTGGATTTCTAA+GGG | 0.577860 | 5.2:+13989355 | MS.gene67684:CDS |
| CTAAGGGAAGTTATGAAGCA+GGG | 0.583061 | 5.2:+13989371 | MS.gene67684:CDS |
| TGATATCCCGCTAAAGACAC+TGG | 0.590824 | 5.2:-13989112 | None:intergenic |
| ACAACCACCAGTGTCTTTAG+CGG | 0.593632 | 5.2:+13989105 | MS.gene67684:CDS |
| GAGACAAGGTGGGAAAGAGA+AGG | 0.596540 | 5.2:+13989393 | MS.gene67684:CDS |
| ACACAATGATCCTCTCCCAC+TGG | 0.608376 | 5.2:-13989467 | None:intergenic |
| AGTTATGAAGCAGGGAGACA+AGG | 0.608744 | 5.2:+13989379 | MS.gene67684:CDS |
| CTATGAAGAAATTTACCAGT+GGG | 0.611053 | 5.2:+13989452 | MS.gene67684:CDS |
| TTCTTCATAGAATCCAACGT+TGG | 0.612106 | 5.2:-13989439 | None:intergenic |
| TATGAAGCAGGGAGACAAGG+TGG | 0.615974 | 5.2:+13989382 | MS.gene67684:CDS |
| TTGATGCAACACTATCCGAA+AGG | 0.634236 | 5.2:+13989494 | MS.gene67684:CDS |
| GCTAGAAGAAAGCACAGAAG+GGG | 0.641612 | 5.2:+13989199 | MS.gene67684:CDS |
| ATGAAGCAGGGAGACAAGGT+GGG | 0.641635 | 5.2:+13989383 | MS.gene67684:CDS |
| TCGATCACCTACAACACATG+TGG | 0.650680 | 5.2:+13989156 | MS.gene67684:CDS |
| TCTATGAAGAAATTTACCAG+TGG | 0.673132 | 5.2:+13989451 | MS.gene67684:CDS |
| TATCCCGCTAAAGACACTGG+TGG | 0.724982 | 5.2:-13989109 | None:intergenic |
| TGTGTTGTAGGTGATCGACA+AGG | 0.755971 | 5.2:-13989151 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGGACAAGAAGTTACATAA+AGG | + | chr5.2:13989304-13989323 | MS.gene67684:CDS | 30.0% | |
| AGGACAAGAAGTTACATAAA+GGG | + | chr5.2:13989305-13989324 | MS.gene67684:CDS | 30.0% | |
| CAAGAAGAAGAAGAAAAAGA+AGG | + | chr5.2:13989285-13989304 | MS.gene67684:CDS | 30.0% | |
| CTATGAAGAAATTTACCAGT+GGG | + | chr5.2:13989452-13989471 | MS.gene67684:CDS | 30.0% | |
| TCTATGAAGAAATTTACCAG+TGG | + | chr5.2:13989451-13989470 | MS.gene67684:CDS | 30.0% | |
| AATGAAACCACATGTGTTGT+AGG | - | chr5.2:13989166-13989185 | None:intergenic | 35.0% | |
| CAAACAAAAACGTTCTGCTT+TGG | + | chr5.2:13989344-13989363 | MS.gene67684:CDS | 35.0% | |
| TGTGGTTTCATTATTCCCTA+AGG | + | chr5.2:13989174-13989193 | MS.gene67684:CDS | 35.0% | |
| TTCTTCATAGAATCCAACGT+TGG | - | chr5.2:13989442-13989461 | None:intergenic | 35.0% | |
| ! | CAAAGCAGAACGTTTTTGTT+TGG | - | chr5.2:13989346-13989365 | None:intergenic | 35.0% |
| ! | GTTCTGCTTTGGATTTCTAA+GGG | + | chr5.2:13989355-13989374 | MS.gene67684:CDS | 35.0% |
| AAGAAATTTACCAGTGGGAG+AGG | + | chr5.2:13989457-13989476 | MS.gene67684:CDS | 40.0% | |
| ATCGACAAGGACTAAGAGTT+GGG | - | chr5.2:13989141-13989160 | None:intergenic | 40.0% | |
| CTAAGGGAAGTTATGAAGCA+GGG | + | chr5.2:13989371-13989390 | MS.gene67684:CDS | 40.0% | |
| TCATAGAATCCAACGTTGGA+GGG | - | chr5.2:13989438-13989457 | None:intergenic | 40.0% | |
| TCTAAGGGAAGTTATGAAGC+AGG | + | chr5.2:13989370-13989389 | MS.gene67684:CDS | 40.0% | |
| TTCATAGAATCCAACGTTGG+AGG | - | chr5.2:13989439-13989458 | None:intergenic | 40.0% | |
| TTGATGCAACACTATCCGAA+AGG | + | chr5.2:13989494-13989513 | MS.gene67684:CDS | 40.0% | |
| ! | AAGCTAGAAGAAAGCACAGA+AGG | + | chr5.2:13989197-13989216 | MS.gene67684:CDS | 40.0% |
| ! | AGCTAGAAGAAAGCACAGAA+GGG | + | chr5.2:13989198-13989217 | MS.gene67684:CDS | 40.0% |
| ! | CGTTCTGCTTTGGATTTCTA+AGG | + | chr5.2:13989354-13989373 | MS.gene67684:CDS | 40.0% |
| !! | GCTTTCTTCTAGCTTCCTTA+GGG | - | chr5.2:13989192-13989211 | None:intergenic | 40.0% |
| !! | TGCTTTCTTCTAGCTTCCTT+AGG | - | chr5.2:13989193-13989212 | None:intergenic | 40.0% |
| !! | TTAGGAGAAGTGGGTTCTTT+TGG | - | chr5.2:13989238-13989257 | None:intergenic | 40.0% |
| ACAACCACCAGTGTCTTTAG+CGG | + | chr5.2:13989105-13989124 | MS.gene67684:CDS | 45.0% | |
| AGTTATGAAGCAGGGAGACA+AGG | + | chr5.2:13989379-13989398 | MS.gene67684:CDS | 45.0% | |
| GATCGACAAGGACTAAGAGT+TGG | - | chr5.2:13989142-13989161 | None:intergenic | 45.0% | |
| TCGATCACCTACAACACATG+TGG | + | chr5.2:13989156-13989175 | MS.gene67684:CDS | 45.0% | |
| TCTCCTAAAGTCTCGTGCAT+GGG | + | chr5.2:13989250-13989269 | MS.gene67684:CDS | 45.0% | |
| TGATATCCCGCTAAAGACAC+TGG | - | chr5.2:13989115-13989134 | None:intergenic | 45.0% | |
| TGTGTTGTAGGTGATCGACA+AGG | - | chr5.2:13989154-13989173 | None:intergenic | 45.0% | |
| TTCTCCTAAAGTCTCGTGCA+TGG | + | chr5.2:13989249-13989268 | MS.gene67684:CDS | 45.0% | |
| ! | CACGAGACTTTAGGAGAAGT+GGG | - | chr5.2:13989247-13989266 | None:intergenic | 45.0% |
| ! | GCTAGAAGAAAGCACAGAAG+GGG | + | chr5.2:13989199-13989218 | MS.gene67684:CDS | 45.0% |
| ACACAATGATCCTCTCCCAC+TGG | - | chr5.2:13989470-13989489 | None:intergenic | 50.0% | |
| AGTGAATGTCCCTCCAACGT+TGG | + | chr5.2:13989426-13989445 | MS.gene67684:CDS | 50.0% | |
| ATGAAGCAGGGAGACAAGGT+GGG | + | chr5.2:13989383-13989402 | MS.gene67684:CDS | 50.0% | |
| CAACCACCAGTGTCTTTAGC+GGG | + | chr5.2:13989106-13989125 | MS.gene67684:CDS | 50.0% | |
| GAGACAAGGTGGGAAAGAGA+AGG | + | chr5.2:13989393-13989412 | MS.gene67684:CDS | 50.0% | |
| TATCCCGCTAAAGACACTGG+TGG | - | chr5.2:13989112-13989131 | None:intergenic | 50.0% | |
| TATGAAGCAGGGAGACAAGG+TGG | + | chr5.2:13989382-13989401 | MS.gene67684:CDS | 50.0% | |
| TCACCCATGCACGAGACTTT+AGG | - | chr5.2:13989256-13989275 | None:intergenic | 50.0% | |
| ! | GCACGAGACTTTAGGAGAAG+TGG | - | chr5.2:13989248-13989267 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 13989055 | 13989519 | 13989055 | ID=MS.gene67684 |
| chr5.2 | mRNA | 13989055 | 13989519 | 13989055 | ID=MS.gene67684.t1;Parent=MS.gene67684 |
| chr5.2 | exon | 13989055 | 13989519 | 13989055 | ID=MS.gene67684.t1.exon1;Parent=MS.gene67684.t1 |
| chr5.2 | CDS | 13989055 | 13989519 | 13989055 | ID=cds.MS.gene67684.t1;Parent=MS.gene67684.t1 |
| Gene Sequence |
| Protein sequence |
