Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67783.t1 | XP_013458798.1 | 91.6 | 178 | 15 | 0 | 1 | 178 | 1 | 178 | 6.60E-84 | 320.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67783.t1 | Q54CI5 | 29.1 | 182 | 89 | 4 | 10 | 176 | 14 | 170 | 4.9e-11 | 69.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67783.t1 | A0A072V3W3 | 91.6 | 178 | 15 | 0 | 1 | 178 | 1 | 178 | 4.7e-84 | 320.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene67783 | MS.gene82121 | PPI |
| MS.gene67783 | MS.gene83474 | PPI |
| MS.gene67783 | MS.gene028111 | PPI |
| MS.gene67783 | MS.gene72809 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67783.t1 | MTR_3g006810 | 91.573 | 178 | 15 | 0 | 1 | 178 | 1 | 178 | 1.37e-119 | 335 |
| MS.gene67783.t1 | MTR_6g003930 | 90.449 | 178 | 17 | 0 | 1 | 178 | 1 | 178 | 5.83e-118 | 331 |
| MS.gene67783.t1 | MTR_6g003930 | 90.449 | 178 | 17 | 0 | 1 | 178 | 73 | 250 | 1.77e-116 | 330 |
| MS.gene67783.t1 | MTR_4g074580 | 79.699 | 133 | 26 | 1 | 1 | 132 | 1 | 133 | 7.08e-72 | 213 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67783.t1 | AT4G10130 | 50.568 | 176 | 80 | 2 | 1 | 176 | 1 | 169 | 3.21e-59 | 182 |
| MS.gene67783.t1 | AT4G10130 | 50.568 | 176 | 80 | 2 | 1 | 176 | 1 | 169 | 3.21e-59 | 182 |
| MS.gene67783.t1 | AT4G10130 | 50.568 | 176 | 80 | 2 | 1 | 176 | 1 | 169 | 3.21e-59 | 182 |
| MS.gene67783.t1 | AT4G10130 | 50.568 | 176 | 80 | 2 | 1 | 176 | 1 | 169 | 3.21e-59 | 182 |
| MS.gene67783.t1 | AT4G10130 | 50.568 | 176 | 80 | 2 | 1 | 176 | 1 | 169 | 3.21e-59 | 182 |
Find 34 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGAAGATTTAAGCTTAC+AGG | 0.214140 | 3.3:+2773949 | MS.gene67783:CDS |
| CTCGTTGGAATTGCTGAAAA+TGG | 0.258071 | 3.3:+2774048 | MS.gene67783:CDS |
| AGATTTAAGCTTACAGGATA+TGG | 0.297921 | 3.3:+2773955 | MS.gene67783:CDS |
| AATGTCTTCAATAGCTTATC+AGG | 0.336890 | 3.3:-2773782 | None:intergenic |
| GGGTATTTCTTATTGAGAAA+TGG | 0.357236 | 3.3:+2774070 | MS.gene67783:CDS |
| GCTCCTTATCATATAATAAA+CGG | 0.369390 | 3.3:-2773889 | None:intergenic |
| AAGAAATTCGTGCTAGTTAC+CGG | 0.375273 | 3.3:+2773738 | MS.gene67783:CDS |
| ATCCCGTTTATTATATGATA+AGG | 0.385875 | 3.3:+2773886 | MS.gene67783:CDS |
| TCGTTGGAATTGCTGAAAAT+GGG | 0.404006 | 3.3:+2774049 | MS.gene67783:CDS |
| TTATGAGGTTCTCAATGTTA+AGG | 0.412571 | 3.3:+2773700 | MS.gene67783:CDS |
| CTCAATGGTGATACTTTACC+AGG | 0.416326 | 3.3:+2774109 | MS.gene67783:CDS |
| AGCTCGTCTGATACTCAGTA+TGG | 0.427425 | 3.3:+2774168 | MS.gene67783:CDS |
| CTCCTTATCATATAATAAAC+GGG | 0.433842 | 3.3:-2773888 | None:intergenic |
| GAGATTTCTCAAAGTTCAAA+AGG | 0.446313 | 3.3:+2773838 | MS.gene67783:CDS |
| TTCTCAAAGTTCAAAAGGCA+TGG | 0.453193 | 3.3:+2773843 | MS.gene67783:CDS |
| CGTACTTGTTTGATCACTGT+TGG | 0.461429 | 3.3:-2773813 | None:intergenic |
| CATTGAGGAAACTCATTATG+AGG | 0.462336 | 3.3:+2773685 | MS.gene67783:CDS |
| AAGATCAAGGCGGGATGCCT+TGG | 0.472867 | 3.3:+2773916 | MS.gene67783:CDS |
| CAAGGAAGAATCACGGATCC+TGG | 0.489467 | 3.3:-2774127 | None:intergenic |
| TTCTGCAATTTCAGCAGCCA+AGG | 0.518010 | 3.3:-2773933 | None:intergenic |
| AATAGTATATCTATACTCAA+TGG | 0.536490 | 3.3:+2774094 | MS.gene67783:CDS |
| TCTTGACAAGCATGACATTG+AGG | 0.539456 | 3.3:+2773670 | MS.gene67783:CDS |
| TCTCAAAGTTCAAAAGGCAT+GGG | 0.541609 | 3.3:+2773844 | MS.gene67783:CDS |
| TTACTTCTCTCTCGACTCGT+TGG | 0.542824 | 3.3:+2774033 | MS.gene67783:CDS |
| ATAAGGAGCTGCAAAGATCA+AGG | 0.544696 | 3.3:+2773903 | MS.gene67783:CDS |
| GGAGCTGCAAAGATCAAGGC+GGG | 0.575943 | 3.3:+2773907 | MS.gene67783:CDS |
| GGATCCGTGATTCTTCCTTG+TGG | 0.587718 | 3.3:+2774130 | MS.gene67783:CDS |
| CAACAGTGATCAAACAAGTA+CGG | 0.595965 | 3.3:+2773814 | MS.gene67783:CDS |
| CGTTGGAATTGCTGAAAATG+GGG | 0.627347 | 3.3:+2774050 | MS.gene67783:CDS |
| AGATCCACAAGGAAGAATCA+CGG | 0.662581 | 3.3:-2774134 | None:intergenic |
| GAGAGAAGTAATCACCACAT+CGG | 0.695257 | 3.3:-2774021 | None:intergenic |
| GTAAACTGAGTACAGCACTC+CGG | 0.699863 | 3.3:-2773757 | None:intergenic |
| TTTAACGAACAAGATCCACA+AGG | 0.727941 | 3.3:-2774145 | None:intergenic |
| AGGAGCTGCAAAGATCAAGG+CGG | 0.734461 | 3.3:+2773906 | MS.gene67783:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAGTATATCTATACTCAA+TGG | + | chr3.3:2774094-2774113 | MS.gene67783:CDS | 20.0% |
| ! | ATCCCGTTTATTATATGATA+AGG | + | chr3.3:2773886-2773905 | MS.gene67783:CDS | 25.0% |
| ! | CTCCTTATCATATAATAAAC+GGG | - | chr3.3:2773891-2773910 | None:intergenic | 25.0% |
| ! | GCTCCTTATCATATAATAAA+CGG | - | chr3.3:2773892-2773911 | None:intergenic | 25.0% |
| AATGTCTTCAATAGCTTATC+AGG | - | chr3.3:2773785-2773804 | None:intergenic | 30.0% | |
| AGATTTAAGCTTACAGGATA+TGG | + | chr3.3:2773955-2773974 | MS.gene67783:CDS | 30.0% | |
| GAGATTTCTCAAAGTTCAAA+AGG | + | chr3.3:2773838-2773857 | MS.gene67783:CDS | 30.0% | |
| GGGTATTTCTTATTGAGAAA+TGG | + | chr3.3:2774070-2774089 | MS.gene67783:CDS | 30.0% | |
| TTATGAGGTTCTCAATGTTA+AGG | + | chr3.3:2773700-2773719 | MS.gene67783:CDS | 30.0% | |
| AAGAAATTCGTGCTAGTTAC+CGG | + | chr3.3:2773738-2773757 | MS.gene67783:CDS | 35.0% | |
| CAACAGTGATCAAACAAGTA+CGG | + | chr3.3:2773814-2773833 | MS.gene67783:CDS | 35.0% | |
| CATTGAGGAAACTCATTATG+AGG | + | chr3.3:2773685-2773704 | MS.gene67783:CDS | 35.0% | |
| TCGTTGGAATTGCTGAAAAT+GGG | + | chr3.3:2774049-2774068 | MS.gene67783:CDS | 35.0% | |
| TGCAGAAGATTTAAGCTTAC+AGG | + | chr3.3:2773949-2773968 | MS.gene67783:CDS | 35.0% | |
| TTCTCAAAGTTCAAAAGGCA+TGG | + | chr3.3:2773843-2773862 | MS.gene67783:CDS | 35.0% | |
| TTTAACGAACAAGATCCACA+AGG | - | chr3.3:2774148-2774167 | None:intergenic | 35.0% | |
| ! | TCTCAAAGTTCAAAAGGCAT+GGG | + | chr3.3:2773844-2773863 | MS.gene67783:CDS | 35.0% |
| AGATCCACAAGGAAGAATCA+CGG | - | chr3.3:2774137-2774156 | None:intergenic | 40.0% | |
| ATAAGGAGCTGCAAAGATCA+AGG | + | chr3.3:2773903-2773922 | MS.gene67783:CDS | 40.0% | |
| CGTACTTGTTTGATCACTGT+TGG | - | chr3.3:2773816-2773835 | None:intergenic | 40.0% | |
| CGTTGGAATTGCTGAAAATG+GGG | + | chr3.3:2774050-2774069 | MS.gene67783:CDS | 40.0% | |
| CTCAATGGTGATACTTTACC+AGG | + | chr3.3:2774109-2774128 | MS.gene67783:CDS | 40.0% | |
| CTCGTTGGAATTGCTGAAAA+TGG | + | chr3.3:2774048-2774067 | MS.gene67783:CDS | 40.0% | |
| GAGAGAAGTAATCACCACAT+CGG | - | chr3.3:2774024-2774043 | None:intergenic | 40.0% | |
| TCTTGACAAGCATGACATTG+AGG | + | chr3.3:2773670-2773689 | MS.gene67783:CDS | 40.0% | |
| ! | CTGTTTTATCAATGCCGATG+TGG | + | chr3.3:2774007-2774026 | MS.gene67783:CDS | 40.0% |
| AGCTCGTCTGATACTCAGTA+TGG | + | chr3.3:2774168-2774187 | MS.gene67783:CDS | 45.0% | |
| GTAAACTGAGTACAGCACTC+CGG | - | chr3.3:2773760-2773779 | None:intergenic | 45.0% | |
| TTCTGCAATTTCAGCAGCCA+AGG | - | chr3.3:2773936-2773955 | None:intergenic | 45.0% | |
| ! | TTACTTCTCTCTCGACTCGT+TGG | + | chr3.3:2774033-2774052 | MS.gene67783:CDS | 45.0% |
| AGGAGCTGCAAAGATCAAGG+CGG | + | chr3.3:2773906-2773925 | MS.gene67783:CDS | 50.0% | |
| CAAGGAAGAATCACGGATCC+TGG | - | chr3.3:2774130-2774149 | None:intergenic | 50.0% | |
| GGATCCGTGATTCTTCCTTG+TGG | + | chr3.3:2774130-2774149 | MS.gene67783:CDS | 50.0% | |
| AAGATCAAGGCGGGATGCCT+TGG | + | chr3.3:2773916-2773935 | MS.gene67783:CDS | 55.0% | |
| GGAGCTGCAAAGATCAAGGC+GGG | + | chr3.3:2773907-2773926 | MS.gene67783:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 2773665 | 2774201 | 2773665 | ID=MS.gene67783 |
| chr3.3 | mRNA | 2773665 | 2774201 | 2773665 | ID=MS.gene67783.t1;Parent=MS.gene67783 |
| chr3.3 | exon | 2773665 | 2774201 | 2773665 | ID=MS.gene67783.t1.exon1;Parent=MS.gene67783.t1 |
| chr3.3 | CDS | 2773665 | 2774201 | 2773665 | ID=cds.MS.gene67783.t1;Parent=MS.gene67783.t1 |
| Gene Sequence |
| Protein sequence |
