AlfalfaGEDB | MS.gene68101

M. sativa cultivar XinJiangDaYe / MS.gene68101

KEGG: K11252
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68101.t1	XP_013469298.1	100	137	0	0	1	137	1	137	8.20E-42	179.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68101.t1	Q1S9I9	100.0	91	0	0	47	137	58	148	3.7e-43	175.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68101.t1	I3S6Z4	100.0	137	0	0	1	137	1	137	5.9e-42	179.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04972	MS.gene68101	0.804327	2.28E-49	-1.69E-46
MS.gene051263	MS.gene68101	0.856752	2.60E-62	-1.69E-46
MS.gene054643	MS.gene68101	0.816697	4.84E-52	-1.69E-46
MS.gene055957	MS.gene68101	0.837577	4.79E-57	-1.69E-46
MS.gene058818	MS.gene68101	0.819485	1.13E-52	-1.69E-46
MS.gene058844	MS.gene68101	0.806148	9.47E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68101.t1	MTR_1g492910	99.270	137	1	0	1	137	1	137	1.15e-94	269
MS.gene68101.t1	MTR_1g090780	98.540	137	2	0	1	137	1	137	6.53e-94	267
MS.gene68101.t1	MTR_4g063240	86.395	147	10	3	1	137	1	147	4.05e-81	235
MS.gene68101.t1	MTR_4g063220	87.162	148	8	3	1	137	1	148	2.42e-77	226
MS.gene68101.t1	MTR_4g063990	87.162	148	8	3	1	137	1	148	2.42e-77	226
MS.gene68101.t1	MTR_4g063200	87.162	148	8	3	1	137	1	148	2.42e-77	226
MS.gene68101.t1	MTR_4g064020	87.162	148	8	3	1	137	1	148	2.42e-77	226
MS.gene68101.t1	MTR_4g071180	87.162	148	8	3	1	137	1	148	2.25e-76	223
MS.gene68101.t1	MTR_4g070240	87.162	148	8	3	1	137	1	148	2.25e-76	223
MS.gene68101.t1	MTR_4g088025	88.811	143	10	3	1	137	1	143	2.86e-75	220
MS.gene68101.t1	MTR_4g070245	85.811	148	10	3	1	137	1	148	1.11e-74	219
MS.gene68101.t1	MTR_4g063275	84.459	148	12	3	1	137	1	148	4.95e-74	217
MS.gene68101.t1	MTR_4g064983	86.111	144	9	3	1	133	1	144	1.26e-72	217
MS.gene68101.t1	MTR_7g099960	95.413	109	4	1	29	137	31	138	2.63e-64	192
MS.gene68101.t1	MTR_1g068600	93.578	109	5	1	29	137	30	136	3.95e-63	189
MS.gene68101.t1	MTR_2g084480	61.798	89	34	0	48	136	136	224	2.02e-37	127
MS.gene68101.t1	MTR_8g069990	68.539	89	25	1	8	96	3	88	2.43e-36	120
MS.gene68101.t1	MTR_4g063270	90.196	51	5	0	75	125	1	51	5.66e-27	95.5
MS.gene68101.t1	MTR_7g114580	90.244	41	4	0	97	137	11	51	6.05e-20	77.4
MS.gene68101.t1	MTR_4g032777	36.364	99	58	1	28	121	12	110	4.23e-13	62.4
MS.gene68101.t1	MTR_2g083800	67.273	55	15	2	8	61	11	63	1.62e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68101.t1	AT2G37470	87.681	138	15	2	1	136	1	138	1.97e-74	218
MS.gene68101.t1	AT3G45980	82.667	150	13	3	1	137	1	150	1.15e-73	216
MS.gene68101.t1	AT2G28720	81.457	151	14	2	1	137	1	151	1.69e-73	216
MS.gene68101.t1	AT1G07790	79.730	148	19	1	1	137	1	148	2.30e-71	211
MS.gene68101.t1	AT3G53650	85.612	139	17	3	1	137	1	138	5.59e-71	209
MS.gene68101.t1	AT5G59910	86.567	134	16	2	6	137	17	150	3.26e-69	205
MS.gene68101.t1	AT3G46030	88.372	129	13	2	11	137	17	145	9.76e-69	204
MS.gene68101.t1	AT5G02570	84.173	139	13	2	1	137	1	132	3.20e-66	197
MS.gene68101.t1	AT5G22880	79.577	142	15	2	10	137	4	145	8.16e-66	196
MS.gene68101.t1	AT3G09480	79.562	137	17	2	1	137	1	126	2.09e-62	187
MS.gene68101.t1	AT1G08170	56.989	93	40	0	42	134	143	235	7.49e-38	129

Find 34 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTCAAGAGCTAGTGAATT+TGG	0.197215	1.1:-66335501	None:intergenic
GTTTCTCTTCTTCTTCTTAT+CGG	0.211855	1.1:-66335213	None:intergenic
GCTGTTCGTCTTGTTCTTCC+TGG	0.277831	1.1:+66335434	MS.gene68101:CDS
CTTGAAGATGTAGATCTTGT+AGG	0.279657	1.1:-66335252	None:intergenic
TCGGTTGAGGATGCTTCCTT+TGG	0.316023	1.1:-66335194	None:intergenic
GCAGGTTTCTTCTCTCCCTT+TGG	0.338176	1.1:-66335113	None:intergenic
TCTGCTGGTGCCTCCTCTGC+GGG	0.374166	1.1:-66335146	None:intergenic
CTTGTTGTATCGAGCAAGTC+TGG	0.381987	1.1:-66335378	None:intergenic
CGGTATCTCAAGCAAGGCTA+TGG	0.396652	1.1:+66335301	MS.gene68101:CDS
CTTCTTCTTCTTATCGGTTG+AGG	0.416596	1.1:-66335207	None:intergenic
AGTGAATTTGGTGACAGCTT+TGG	0.420989	1.1:-66335489	None:intergenic
TCTGCGGGCTTCTTCTCAGC+AGG	0.422937	1.1:-66335131	None:intergenic
CTACAAGATCTACATCTTCA+AGG	0.431521	1.1:+66335253	MS.gene68101:CDS
CGGTTGAGGATGCTTCCTTT+GGG	0.432726	1.1:-66335193	None:intergenic
TCCCTCGGAGACAGCGTGCT+TGG	0.447024	1.1:-66335465	None:intergenic
CATCTTCAAGGTTCTCAAGC+AGG	0.470454	1.1:+66335265	MS.gene68101:CDS
GCGTGCTTGGCGAGTTCACC+AGG	0.485011	1.1:-66335452	None:intergenic
GGTGACAGCTTTGGTTCCCT+CGG	0.503972	1.1:-66335480	None:intergenic
TCAATCAACAATGGCACCAA+AGG	0.515027	1.1:+66335097	None:intergenic
CATCTTCGAGAAGCTCGCTC+AGG	0.521238	1.1:+66335349	MS.gene68101:CDS
GGTATCTCAAGCAAGGCTAT+GGG	0.525343	1.1:+66335302	MS.gene68101:CDS
CTCTGCTGGTGCCTCCTCTG+CGG	0.528843	1.1:-66335147	None:intergenic
TTGCTTGAGATACCGATGTC+AGG	0.547522	1.1:-66335293	None:intergenic
CAATCAACAATGGCACCAAA+GGG	0.556792	1.1:+66335098	None:intergenic
CTGAATCTCACGAGAAGTGA+TGG	0.558881	1.1:-66335408	None:intergenic
TGAGAAGAAGCCCGCAGAGG+AGG	0.559076	1.1:+66335136	MS.gene68101:CDS
GCCAAGCACGCTGTCTCCGA+GGG	0.566568	1.1:+66335464	MS.gene68101:CDS
CGCCAAGCACGCTGTCTCCG+AGG	0.573257	1.1:+66335463	MS.gene68101:CDS
TGACATCGGTATCTCAAGCA+AGG	0.623015	1.1:+66335295	MS.gene68101:CDS
AAGCAGGTTCATCCTGACAT+CGG	0.661631	1.1:+66335281	MS.gene68101:CDS
ATCTCACGAGAAGTGATGGT+AGG	0.665630	1.1:-66335404	None:intergenic
AGCAGAGAAGAAGATCCCAA+AGG	0.665852	1.1:+66335178	MS.gene68101:CDS
GTATCTCAAGCAAGGCTATG+GGG	0.674185	1.1:+66335303	MS.gene68101:CDS
TGCTGAGAAGAAGCCCGCAG+AGG	0.683113	1.1:+66335133	MS.gene68101:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTTTCTCTTCTTCTTCTTAT+CGG	-	chr1.1:66335216-66335235	None:intergenic	30.0%
	CTACAAGATCTACATCTTCA+AGG	+	chr1.1:66335253-66335272	MS.gene68101:CDS	35.0%
	CTTGAAGATGTAGATCTTGT+AGG	-	chr1.1:66335255-66335274	None:intergenic	35.0%
!	AGTGAATTTGGTGACAGCTT+TGG	-	chr1.1:66335492-66335511	None:intergenic	40.0%
!	CTTCTTCTTCTTATCGGTTG+AGG	-	chr1.1:66335210-66335229	None:intergenic	40.0%
	AAGCAGGTTCATCCTGACAT+CGG	+	chr1.1:66335281-66335300	MS.gene68101:CDS	45.0%
	AGCAGAGAAGAAGATCCCAA+AGG	+	chr1.1:66335178-66335197	MS.gene68101:CDS	45.0%
	ATCTCACGAGAAGTGATGGT+AGG	-	chr1.1:66335407-66335426	None:intergenic	45.0%
	CATCTTCAAGGTTCTCAAGC+AGG	+	chr1.1:66335265-66335284	MS.gene68101:CDS	45.0%
	CTGAATCTCACGAGAAGTGA+TGG	-	chr1.1:66335411-66335430	None:intergenic	45.0%
	GGTATCTCAAGCAAGGCTAT+GGG	+	chr1.1:66335302-66335321	MS.gene68101:CDS	45.0%
	GTATCTCAAGCAAGGCTATG+GGG	+	chr1.1:66335303-66335322	MS.gene68101:CDS	45.0%
	TGACATCGGTATCTCAAGCA+AGG	+	chr1.1:66335295-66335314	MS.gene68101:CDS	45.0%
	TTGCTTGAGATACCGATGTC+AGG	-	chr1.1:66335296-66335315	None:intergenic	45.0%
!	CTTGTTGTATCGAGCAAGTC+TGG	-	chr1.1:66335381-66335400	None:intergenic	45.0%
!	TCTGCTTTCGTTTTCTCTGC+TGG	-	chr1.1:66335164-66335183	None:intergenic	45.0%
	CGGTATCTCAAGCAAGGCTA+TGG	+	chr1.1:66335301-66335320	MS.gene68101:CDS	50.0%
	CGGTTGAGGATGCTTCCTTT+GGG	-	chr1.1:66335196-66335215	None:intergenic	50.0%
	GCAGGTTTCTTCTCTCCCTT+TGG	-	chr1.1:66335116-66335135	None:intergenic	50.0%
	TCGGTTGAGGATGCTTCCTT+TGG	-	chr1.1:66335197-66335216	None:intergenic	50.0%
!	GCTGTTCGTCTTGTTCTTCC+TGG	+	chr1.1:66335434-66335453	MS.gene68101:CDS	50.0%
	CATCTTCGAGAAGCTCGCTC+AGG	+	chr1.1:66335349-66335368	MS.gene68101:CDS	55.0%
!	GGTGACAGCTTTGGTTCCCT+CGG	-	chr1.1:66335483-66335502	None:intergenic	55.0%
	TCTGCGGGCTTCTTCTCAGC+AGG	-	chr1.1:66335134-66335153	None:intergenic	60.0%
	TGAGAAGAAGCCCGCAGAGG+AGG	+	chr1.1:66335136-66335155	MS.gene68101:CDS	60.0%
	TGCTGAGAAGAAGCCCGCAG+AGG	+	chr1.1:66335133-66335152	MS.gene68101:CDS	60.0%
	CTCTGCTGGTGCCTCCTCTG+CGG	-	chr1.1:66335150-66335169	None:intergenic	65.0%
	GCCAAGCACGCTGTCTCCGA+GGG	+	chr1.1:66335464-66335483	MS.gene68101:CDS	65.0%
	GCGTGCTTGGCGAGTTCACC+AGG	-	chr1.1:66335455-66335474	None:intergenic	65.0%
	TCCCTCGGAGACAGCGTGCT+TGG	-	chr1.1:66335468-66335487	None:intergenic	65.0%
	TCTGCTGGTGCCTCCTCTGC+GGG	-	chr1.1:66335149-66335168	None:intergenic	65.0%
	CGCCAAGCACGCTGTCTCCG+AGG	+	chr1.1:66335463-66335482	MS.gene68101:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	66335107	66335520	66335107	ID=MS.gene68101
chr1.1	mRNA	66335107	66335520	66335107	ID=MS.gene68101.t1;Parent=MS.gene68101
chr1.1	exon	66335107	66335520	66335107	ID=MS.gene68101.t1.exon1;Parent=MS.gene68101.t1
chr1.1	CDS	66335107	66335520	66335107	ID=cds.MS.gene68101.t1;Parent=MS.gene68101.t1

Gene Sequence

>MS.gene68101

ATGGCACCAAAGGGAGAGAAGAAACCTGCTGAGAAGAAGCCCGCAGAGGAGGCACCAGCAGAGAAAACGAAAGCAGAGAAGAAGATCCCAAAGGAAGCATCCTCAACCGATAAGAAGAAGAAGAGAAACAAGAAGAGCGTTGAAACCTACAAGATCTACATCTTCAAGGTTCTCAAGCAGGTTCATCCTGACATCGGTATCTCAAGCAAGGCTATGGGGATCATGAACAGCTTCATCAACGACATCTTCGAGAAGCTCGCTCAGGAATCTTCCAGACTTGCTCGATACAACAAGAAGCCTACCATCACTTCTCGTGAGATTCAGACTGCTGTTCGTCTTGTTCTTCCTGGTGAACTCGCCAAGCACGCTGTCTCCGAGGGAACCAAAGCTGTCACCAAATTCACTAGCTCTTGA

Protein sequence

>MS.gene68101.t1

MAPKGEKKPAEKKPAEEAPAEKTKAEKKIPKEASSTDKKKKRNKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS