AlfalfaGEDB | MS.gene68413

M. sativa cultivar XinJiangDaYe / MS.gene68413

KEGG: K16281
GO: -
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68413.t1	XP_003609919.1	79.2	168	23	2	1	156	1	168	6.90E-69	270

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68413.t1	Q9LY41	38.9	90	47	2	43	131	83	165	1.9e-11	70.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68413.t1	G7JR49	79.2	168	23	2	1	156	1	168	5.0e-69	270.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene68413	MS.gene035654	PPI
MS.gene68413	MS.gene018385	PPI
MS.gene68413	MS.gene012454	PPI
MS.gene68413	MS.gene57944	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68413.t1	MTR_4g124380	79.167	168	23	2	1	156	1	168	2.64e-90	260
MS.gene68413.t1	MTR_4g124390	74.684	158	25	2	1	156	1	145	3.56e-80	234
MS.gene68413.t1	MTR_7g078150	36.000	75	42	1	55	129	69	137	1.66e-14	67.8
MS.gene68413.t1	MTR_5g010100	38.667	75	40	2	55	129	60	128	3.89e-14	65.5
MS.gene68413.t1	MTR_8g098770	38.667	75	36	3	55	129	69	133	5.53e-14	65.9
MS.gene68413.t1	MTR_3g082510	41.667	72	36	1	58	129	81	146	9.69e-13	62.4
MS.gene68413.t1	MTR_8g009190	38.710	93	49	2	43	134	71	156	1.19e-12	64.3
MS.gene68413.t1	MTR_2g044570	42.105	76	36	2	57	131	63	131	3.71e-12	62.8
MS.gene68413.t1	MTR_8g028815	32.673	101	57	4	55	152	73	165	7.54e-12	60.5
MS.gene68413.t1	MTR_4g129420	45.614	57	24	1	76	132	144	193	2.86e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68413.t1	AT3G43430	41.176	170	83	6	1	156	1	167	1.32e-36	124
MS.gene68413.t1	AT5G20885	40.341	176	81	6	1	154	1	174	7.21e-36	122
MS.gene68413.t1	AT1G63840	32.653	147	71	6	1	129	1	137	5.52e-18	76.6
MS.gene68413.t1	AT5G41400	39.744	78	41	3	54	130	82	154	8.02e-16	71.2
MS.gene68413.t1	AT4G00305	41.772	79	39	2	55	133	51	122	2.17e-15	68.6
MS.gene68413.t1	AT3G61460	27.820	133	67	3	20	129	15	141	8.42e-15	68.2
MS.gene68413.t1	AT3G60220	38.710	93	49	2	43	134	83	168	1.32e-13	67.0
MS.gene68413.t1	AT2G01150	53.704	54	18	2	76	129	74	120	8.05e-13	62.4
MS.gene68413.t1	AT2G01150	53.704	54	18	2	76	129	100	146	1.18e-12	62.8
MS.gene68413.t1	AT2G01150	53.704	54	18	2	76	129	123	169	1.92e-12	62.4
MS.gene68413.t1	AT1G15100	44.000	75	33	3	76	150	86	151	3.09e-11	58.5
MS.gene68413.t1	AT5G43200	43.077	65	31	1	64	128	144	202	9.29e-11	58.2
MS.gene68413.t1	AT2G04240	36.111	72	39	2	58	129	85	149	9.68e-11	57.4
MS.gene68413.t1	AT2G04240	36.111	72	39	2	58	129	85	149	9.68e-11	57.4

Find 25 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCACATGTGTCTTCTGTTTC+TGG	0.130755	8.2:+9144809	None:intergenic
ATGTTACCGTTGACGAGTTT+TGG	0.271368	8.2:-9144851	MS.gene68413:CDS
TTGTTTGAACAAGCTGAAAA+TGG	0.301105	8.2:-9144783	MS.gene68413:CDS
CAAGAGTGTTGTTGTTGGTT+TGG	0.363777	8.2:+9144881	None:intergenic
CTCTACAAAGTGGACAAGTT+TGG	0.375846	8.2:+9144663	None:intergenic
AACAAATTATTTAATTAAGA+AGG	0.386010	8.2:+9144551	None:intergenic
GGTTAGCACAGCAATGTATA+TGG	0.401321	8.2:+9144967	None:intergenic
ATTCACCTAGTCAACCTCAT+TGG	0.420746	8.2:-9144616	MS.gene68413:CDS
TTCACCTAGTCAACCTCATT+GGG	0.441403	8.2:-9144615	MS.gene68413:CDS
TAACCATCGCTGTTCTTAGA+TGG	0.454381	8.2:-9144949	MS.gene68413:CDS
TAAAATAGAGATGGATTGTT+CGG	0.456291	8.2:+9145003	None:intergenic
GCCTATTGTTAAAATAGAGA+TGG	0.495744	8.2:+9144994	None:intergenic
CTGTGATCATGTGTTTCATA+AGG	0.517073	8.2:-9144735	MS.gene68413:CDS
GACCACAAGAGTGTTGTTGT+TGG	0.524729	8.2:+9144876	None:intergenic
GCACAGCAATGTATATGGCT+TGG	0.541400	8.2:+9144972	None:intergenic
TAGTCAACCTCATTGGGCTG+TGG	0.577784	8.2:-9144609	MS.gene68413:CDS
ATAAGGAATGCATAGAGAAG+TGG	0.586287	8.2:-9144718	MS.gene68413:CDS
ATTTCTCCAAAACTCGTCAA+CGG	0.592534	8.2:+9144845	None:intergenic
ATCAAAGGAGCTCTACAAAG+TGG	0.596806	8.2:+9144653	None:intergenic
ACAGCCCAATGAGGTTGACT+AGG	0.604880	8.2:+9144611	None:intergenic
GATCCATCTAAGAACAGCGA+TGG	0.623298	8.2:+9144946	None:intergenic
AACCAACAACAACACTCTTG+TGG	0.623502	8.2:-9144878	MS.gene68413:CDS
ATAGAGAAGTGGTTGGAACA+TGG	0.640834	8.2:-9144707	MS.gene68413:CDS
AGTCTTTCCACAGCCCAATG+AGG	0.646393	8.2:+9144602	None:intergenic
GGAATGCATAGAGAAGTGGT+TGG	0.653546	8.2:-9144714	MS.gene68413:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCCTTTGATTAATAGTTTTT+GGG	-	chr8.2:9144942-9144961	MS.gene68413:CDS	20.0%
!	TCCCAAAAACTATTAATCAA+AGG	+	chr8.2:9144946-9144965	None:intergenic	25.0%
!!	CTCCTTTGATTAATAGTTTT+TGG	-	chr8.2:9144941-9144960	MS.gene68413:CDS	25.0%
!!!	GAAAGACTTTTTTATCTATG+TGG	-	chr8.2:9144994-9145013	MS.gene68413:CDS	25.0%
!!!	TAAAATAGAGATGGATTGTT+CGG	+	chr8.2:9144581-9144600	None:intergenic	25.0%
!!!	TCCATCTCTATTTTAACAAT+AGG	-	chr8.2:9144586-9144605	MS.gene68413:CDS	25.0%
	GCCTATTGTTAAAATAGAGA+TGG	+	chr8.2:9144590-9144609	None:intergenic	30.0%
	TTGTTTGAACAAGCTGAAAA+TGG	-	chr8.2:9144798-9144817	MS.gene68413:CDS	30.0%
	ATAAGGAATGCATAGAGAAG+TGG	-	chr8.2:9144863-9144882	MS.gene68413:CDS	35.0%
	ATTTCTCCAAAACTCGTCAA+CGG	+	chr8.2:9144739-9144758	None:intergenic	35.0%
	CTGTGATCATGTGTTTCATA+AGG	-	chr8.2:9144846-9144865	MS.gene68413:CDS	35.0%
	AACCAACAACAACACTCTTG+TGG	-	chr8.2:9144703-9144722	MS.gene68413:CDS	40.0%
	ATAGAGAAGTGGTTGGAACA+TGG	-	chr8.2:9144874-9144893	MS.gene68413:CDS	40.0%
	ATCAAAGGAGCTCTACAAAG+TGG	+	chr8.2:9144931-9144950	None:intergenic	40.0%
	ATTCACCTAGTCAACCTCAT+TGG	-	chr8.2:9144965-9144984	MS.gene68413:CDS	40.0%
	CTCTACAAAGTGGACAAGTT+TGG	+	chr8.2:9144921-9144940	None:intergenic	40.0%
	GGTTAGCACAGCAATGTATA+TGG	+	chr8.2:9144617-9144636	None:intergenic	40.0%
	TAACCATCGCTGTTCTTAGA+TGG	-	chr8.2:9144632-9144651	MS.gene68413:CDS	40.0%
	TTCACCTAGTCAACCTCATT+GGG	-	chr8.2:9144966-9144985	MS.gene68413:CDS	40.0%
!	ATGTTACCGTTGACGAGTTT+TGG	-	chr8.2:9144730-9144749	MS.gene68413:CDS	40.0%
!!	CAAGAGTGTTGTTGTTGGTT+TGG	+	chr8.2:9144703-9144722	None:intergenic	40.0%
!!!	GACGAGTTTTGGAGAAATCA+AGG	-	chr8.2:9144741-9144760	MS.gene68413:CDS	40.0%
	GATCCATCTAAGAACAGCGA+TGG	+	chr8.2:9144638-9144657	None:intergenic	45.0%
	GCACAGCAATGTATATGGCT+TGG	+	chr8.2:9144612-9144631	None:intergenic	45.0%
	GCACATGTGTCTTCTGTTTC+TGG	+	chr8.2:9144775-9144794	None:intergenic	45.0%
	GGAATGCATAGAGAAGTGGT+TGG	-	chr8.2:9144867-9144886	MS.gene68413:CDS	45.0%
!!	GACCACAAGAGTGTTGTTGT+TGG	+	chr8.2:9144708-9144727	None:intergenic	45.0%
	ACAGCCCAATGAGGTTGACT+AGG	+	chr8.2:9144973-9144992	None:intergenic	50.0%
	AGTCTTTCCACAGCCCAATG+AGG	+	chr8.2:9144982-9145001	None:intergenic	50.0%
	TAGTCAACCTCATTGGGCTG+TGG	-	chr8.2:9144972-9144991	MS.gene68413:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	9144565	9145038	9144565	ID=MS.gene68413
chr8.2	mRNA	9144565	9145038	9144565	ID=MS.gene68413.t1;Parent=MS.gene68413
chr8.2	exon	9144565	9145038	9144565	ID=MS.gene68413.t1.exon1;Parent=MS.gene68413.t1
chr8.2	CDS	9144565	9145038	9144565	ID=cds.MS.gene68413.t1;Parent=MS.gene68413.t1

Gene Sequence

>MS.gene68413

ATGGGTTTCTACACCGAACAATCCATCTCTATTTTAACAATAGGCCAAGCCATATACATTGCTGTGCTAACCATCGCTGTTCTTAGATGGATCATATGCTTAGTTTTCAAAATAATCGAACATACAAGAACAAAACCAAACCAACAACAACACTCTTGTGGTCAAATGTTACCGTTGACGAGTTTTGGAGAAATCAAGGAAAGACATCCAGAAACAGAAGACACATGTGCTATTTGTTTGAACAAGCTGAAAATGGAAGATGAAGTTAGAGAGTTGATGAACTGTGATCATGTGTTTCATAAGGAATGCATAGAGAAGTGGTTGGAACATGGTTATGAAAATGAAAATCATAACCAAACTTGTCCACTTTGTAGAGCTCCTTTGATTAATAGTTTTTGGGATTCACCTAGTCAACCTCATTGGGCTGTGGAAAGACTTTTTTATCTATGTGGTGATGATCTTTTGCCTTCTTAA

Protein sequence

>MS.gene68413.t1

MGFYTEQSISILTIGQAIYIAVLTIAVLRWIICLVFKIIEHTRTKPNQQQHSCGQMLPLTSFGEIKERHPETEDTCAICLNKLKMEDEVRELMNCDHVFHKECIEKWLEHGYENENHNQTCPLCRAPLINSFWDSPSQPHWAVERLFYLCGDDLLPS