AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68875


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68875.t1 MTR_4g132780 98.404 188 2 1 1 188 1 187 1.39e-128 380
MS.gene68875.t1 MTR_1g012840 41.509 159 92 1 1 158 1 159 5.24e-35 131
MS.gene68875.t1 MTR_2g451540 40.854 164 92 2 5 164 2 164 1.94e-34 129
MS.gene68875.t1 MTR_7g059150 38.400 125 72 2 37 158 92 214 1.37e-20 87.4
MS.gene68875.t1 MTR_2g090135 37.097 124 73 2 37 157 95 216 3.33e-19 83.6
MS.gene68875.t1 MTR_2g090135 38.614 101 57 2 37 134 95 193 4.33e-16 73.9
MS.gene68875.t1 MTR_8g107280 32.203 118 74 2 44 161 483 594 9.94e-12 63.9
MS.gene68875.t1 MTR_2g078020 33.981 103 62 2 47 149 469 565 2.17e-11 63.2
MS.gene68875.t1 MTR_2g078020 33.981 103 62 2 47 149 469 565 3.18e-11 62.4
MS.gene68875.t1 MTR_2g067870 30.702 114 76 2 45 158 7 117 7.97e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68875.t1 AT1G62390 65.957 188 49 3 1 177 1 184 6.09e-78 248
MS.gene68875.t1 AT2G25290 42.520 127 73 0 32 158 44 170 2.43e-30 118
MS.gene68875.t1 AT2G25290 42.520 127 73 0 32 158 44 170 2.43e-30 118
MS.gene68875.t1 AT2G25290 42.520 127 73 0 32 158 44 170 2.43e-30 118
MS.gene68875.t1 AT2G25290 42.520 127 73 0 32 158 44 170 2.43e-30 118
MS.gene68875.t1 AT4G32070 40.769 130 77 0 32 161 43 172 5.10e-29 114
MS.gene68875.t1 AT4G32070 40.769 130 77 0 32 161 43 172 5.10e-29 114
MS.gene68875.t1 AT5G20360 38.514 148 85 2 15 158 39 184 3.74e-26 105
MS.gene68875.t1 AT5G20360 38.514 148 85 2 15 158 39 184 3.74e-26 105
MS.gene68875.t1 AT5G20360 41.803 122 71 0 37 158 123 244 4.28e-26 105
MS.gene68875.t1 AT5G20360 41.803 122 71 0 37 158 123 244 4.28e-26 105
MS.gene68875.t1 AT5G20360 41.803 122 71 0 37 158 113 234 4.43e-26 105
MS.gene68875.t1 AT5G20360 41.803 122 71 0 37 158 33 154 5.69e-26 105
MS.gene68875.t1 AT5G20360 41.803 122 71 0 37 158 33 154 5.69e-26 105
MS.gene68875.t1 AT4G30480 31.098 164 96 3 10 170 87 236 3.72e-15 72.8
MS.gene68875.t1 AT3G58620 31.111 135 87 2 37 171 446 574 1.88e-13 68.9
MS.gene68875.t1 AT3G58620 31.111 135 87 2 37 171 446 574 3.83e-13 68.2
MS.gene68875.t1 AT4G30480 33.594 128 68 3 10 134 87 200 1.00e-11 62.4
MS.gene68875.t1 AT3G17970 33.913 115 70 2 44 158 478 586 5.71e-11 61.6

Find 65 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGTGTACTTTGGCTCTTC+AGG 0.248512 8.4:+1537849 MS.gene68875:CDS
CAACCCCACCATTTGGTGTT+TGG 0.286040 8.4:-1537612 None:intergenic
TCAAGATGTGCAGTTGTTAT+TGG 0.289033 8.4:+1537948 MS.gene68875:CDS
TCCGTGGTGGCTCCTATTGC+TGG 0.296717 8.4:+1538100 MS.gene68875:CDS
ACCAAATGGTGGGGTTGAAT+TGG 0.315838 8.4:+1537618 MS.gene68875:CDS
AGCCCTATCAGGATGTGTTT+TGG 0.326734 8.4:-1537758 None:intergenic
TTCTTCGACGCAGGCCGTGC+TGG 0.341743 8.4:-1538139 None:intergenic
CAGAGATTGAGGGCTGCGTT+TGG 0.343757 8.4:+1538009 MS.gene68875:CDS
CGGCCTTCGCCTGCTGCGTT+AGG 0.354738 8.4:+1538066 MS.gene68875:CDS
AAGAAAATTATGGGGAAATC+AGG 0.361902 8.4:+1537538 None:intergenic
GAATAGAAGGTTTCAGAGTA+AGG 0.367198 8.4:+1537687 MS.gene68875:CDS
CGCCCAAAACACATCCTGAT+AGG 0.369541 8.4:+1537755 MS.gene68875:CDS
GGGCTTTGCTTCGTAGGGCT+CGG 0.370895 8.4:+1537890 MS.gene68875:CDS
AGGTTCAGCCGCAATTTGTC+CGG 0.377457 8.4:+1537869 MS.gene68875:CDS
AGAGATTGAGGGCTGCGTTT+GGG 0.381653 8.4:+1538010 MS.gene68875:CDS
TGAAAGAAGAAGGGAATAGA+AGG 0.392126 8.4:+1537674 MS.gene68875:CDS
GTGGCTCCTATTGCTGGATT+AGG 0.394553 8.4:+1538106 MS.gene68875:CDS
ATCCAAAGCATCCTTATGAT+TGG 0.395798 8.4:-1537980 None:intergenic
TTGTCCGGGCTTTGCTTCGT+AGG 0.400759 8.4:+1537884 MS.gene68875:CDS
TCCCAATCATAAGGATGCTT+TGG 0.400869 8.4:+1537978 MS.gene68875:CDS
TCCAATTCAACCCCACCATT+TGG 0.402916 8.4:-1537619 None:intergenic
ATCACAGATTCATAATCAAT+AGG 0.414155 8.4:-1537823 None:intergenic
TCGTAGGGCTCGGGCGTTTG+AGG 0.421207 8.4:+1537900 MS.gene68875:CDS
TGGATATTGCTCAGAGATTG+AGG 0.427013 8.4:+1537998 MS.gene68875:CDS
TTTCAGAGTAAGGATTATAC+TGG 0.429564 8.4:+1537697 MS.gene68875:CDS
GGCTTTGCTTCGTAGGGCTC+GGG 0.434118 8.4:+1537891 MS.gene68875:CDS
GGCTGCGTTTGGGCCTCGTC+AGG 0.435769 8.4:+1538020 MS.gene68875:CDS
AGCACGGCCTGCGTCGAAGA+AGG 0.437490 8.4:+1538141 MS.gene68875:CDS
GGATATTGCTCAGAGATTGA+GGG 0.451571 8.4:+1537999 MS.gene68875:CDS
GCACATGAAATGAAAGAAGA+AGG 0.462119 8.4:+1537664 MS.gene68875:CDS
TCCAAAGCATCCTTATGATT+GGG 0.467802 8.4:-1537979 None:intergenic
GGCTTCTGATCCCAATCATA+AGG 0.472316 8.4:+1537969 MS.gene68875:CDS
CACGGACCTAATCCAGCAAT+AGG 0.475945 8.4:-1538112 None:intergenic
GCAGAAGCACCTAACGCAGC+AGG 0.476486 8.4:-1538075 None:intergenic
ATCAGGTGGTAGAAGAAAGA+AGG 0.476870 8.4:+1537555 MS.gene68875:CDS
GAGCCCTACGAAGCAAAGCC+CGG 0.482060 8.4:-1537888 None:intergenic
TGTGATTTCTGAGTGTACTT+TGG 0.485055 8.4:+1537840 MS.gene68875:CDS
TAGGTCCGTGTTTGCCAGCA+CGG 0.489653 8.4:+1538125 MS.gene68875:CDS
GCCTCGTCAGGAAGCACAGC+AGG 0.492073 8.4:+1538032 MS.gene68875:CDS
GGTTCAGCCGCAATTTGTCC+GGG 0.494328 8.4:+1537870 MS.gene68875:CDS
TGTCCGGGCTTTGCTTCGTA+GGG 0.499344 8.4:+1537885 MS.gene68875:CDS
GCACGGCCTGCGTCGAAGAA+GGG 0.502293 8.4:+1538142 MS.gene68875:CDS
GGCTGTTGGTAAGTATGAAT+TGG 0.502917 8.4:+1537921 MS.gene68875:CDS
GCACCTAACGCAGCAGGCGA+AGG 0.506022 8.4:-1538069 None:intergenic
GCTTCTGTCCAAACACCAAA+TGG 0.508934 8.4:+1537604 MS.gene68875:CDS
GCTCGGGCGTTTGAGGCTGT+TGG 0.518791 8.4:+1537907 MS.gene68875:CDS
CACGGCCTGCGTCGAAGAAG+GGG 0.522245 8.4:+1538143 MS.gene68875:CDS
AGCAAAGCCCGGACAAATTG+CGG 0.535303 8.4:-1537877 None:intergenic
TCCTGCTGTGCTTCCTGACG+AGG 0.536302 8.4:-1538033 None:intergenic
TCAGGTGGTAGAAGAAAGAA+GGG 0.549260 8.4:+1537556 MS.gene68875:CDS
CACATGAAATGAAAGAAGAA+GGG 0.561194 8.4:+1537665 MS.gene68875:CDS
CAGCAGGCGAAGGCCGACTG+TGG 0.567544 8.4:-1538059 None:intergenic
GCAGGCCGTGCTGGCAAACA+CGG 0.570124 8.4:-1538130 None:intergenic
CTGTCCAAACACCAAATGGT+GGG 0.572237 8.4:+1537608 MS.gene68875:CDS
GCCCAAAACACATCCTGATA+GGG 0.587986 8.4:+1537756 MS.gene68875:CDS
CACAGCAGGATCTCCACAGT+CGG 0.611695 8.4:+1538046 MS.gene68875:CDS
TTAGGTGCTTCTGCTGTCCG+TGG 0.626214 8.4:+1538084 MS.gene68875:CDS
AGGAGAAACAACTGACCCAA+CGG 0.628623 8.4:-1538176 None:intergenic
AAAATTATGGGGAAATCAGG+TGG 0.628733 8.4:+1537541 None:intergenic
TCTGTCCAAACACCAAATGG+TGG 0.630928 8.4:+1537607 MS.gene68875:CDS
TGTCCAAACACCAAATGGTG+GGG 0.637307 8.4:+1537609 MS.gene68875:CDS
ACGGCCTGCGTCGAAGAAGG+GGG 0.639868 8.4:+1538144 MS.gene68875:CDS
TCCAGCAATAGGAGCCACCA+CGG 0.645973 8.4:-1538101 None:intergenic
GGTGCTTCTGCTGTCCGTGG+TGG 0.654942 8.4:+1538087 MS.gene68875:CDS
GGTGGTAGAAGAAAGAAGGG+TGG 0.730439 8.4:+1537559 MS.gene68875:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATCACAGATTCATAATCAAT+AGG - chr8.4:1537826-1537845 None:intergenic 25.0%
!! TTCATCAATTTTCTTGAAGA+AGG + chr8.4:1537642-1537661 MS.gene68875:CDS 25.0%
CACATGAAATGAAAGAAGAA+GGG + chr8.4:1537665-1537684 MS.gene68875:CDS 30.0%
TTTCAGAGTAAGGATTATAC+TGG + chr8.4:1537697-1537716 MS.gene68875:CDS 30.0%
GAATAGAAGGTTTCAGAGTA+AGG + chr8.4:1537687-1537706 MS.gene68875:CDS 35.0%
GCACATGAAATGAAAGAAGA+AGG + chr8.4:1537664-1537683 MS.gene68875:CDS 35.0%
TCAAGATGTGCAGTTGTTAT+TGG + chr8.4:1537948-1537967 MS.gene68875:CDS 35.0%
TGAAAGAAGAAGGGAATAGA+AGG + chr8.4:1537674-1537693 MS.gene68875:CDS 35.0%
TGTGATTTCTGAGTGTACTT+TGG + chr8.4:1537840-1537859 MS.gene68875:CDS 35.0%
! ATCCAAAGCATCCTTATGAT+TGG - chr8.4:1537983-1538002 None:intergenic 35.0%
! TCCAAAGCATCCTTATGATT+GGG - chr8.4:1537982-1538001 None:intergenic 35.0%
ATCAGGTGGTAGAAGAAAGA+AGG + chr8.4:1537555-1537574 MS.gene68875:CDS 40.0%
GGATATTGCTCAGAGATTGA+GGG + chr8.4:1537999-1538018 MS.gene68875:CDS 40.0%
TCAGGTGGTAGAAGAAAGAA+GGG + chr8.4:1537556-1537575 MS.gene68875:CDS 40.0%
TCCCAATCATAAGGATGCTT+TGG + chr8.4:1537978-1537997 MS.gene68875:CDS 40.0%
TGGATATTGCTCAGAGATTG+AGG + chr8.4:1537998-1538017 MS.gene68875:CDS 40.0%
! GGCTGTTGGTAAGTATGAAT+TGG + chr8.4:1537921-1537940 MS.gene68875:CDS 40.0%
!!! GGCTGTTTTTCACAGCAATA+GGG + chr8.4:1537777-1537796 MS.gene68875:CDS 40.0%
AGGAGAAACAACTGACCCAA+CGG - chr8.4:1538179-1538198 None:intergenic 45.0%
CTGTCCAAACACCAAATGGT+GGG + chr8.4:1537608-1537627 MS.gene68875:CDS 45.0%
CTGTGAAAAACAGCCCTATC+AGG - chr8.4:1537772-1537791 None:intergenic 45.0%
GCCCAAAACACATCCTGATA+GGG + chr8.4:1537756-1537775 MS.gene68875:CDS 45.0%
GCTTCTGTCCAAACACCAAA+TGG + chr8.4:1537604-1537623 MS.gene68875:CDS 45.0%
GGCTTCTGATCCCAATCATA+AGG + chr8.4:1537969-1537988 MS.gene68875:CDS 45.0%
TCCAATTCAACCCCACCATT+TGG - chr8.4:1537622-1537641 None:intergenic 45.0%
TCTGTCCAAACACCAAATGG+TGG + chr8.4:1537607-1537626 MS.gene68875:CDS 45.0%
TGTCCAAACACCAAATGGTG+GGG + chr8.4:1537609-1537628 MS.gene68875:CDS 45.0%
! ACCAAATGGTGGGGTTGAAT+TGG + chr8.4:1537618-1537637 MS.gene68875:CDS 45.0%
! AGCCCTATCAGGATGTGTTT+TGG - chr8.4:1537761-1537780 None:intergenic 45.0%
! GCCCTATCAGGATGTGTTTT+GGG - chr8.4:1537760-1537779 None:intergenic 45.0%
!! TGAGTGTACTTTGGCTCTTC+AGG + chr8.4:1537849-1537868 MS.gene68875:CDS 45.0%
!!! GGGCTGTTTTTCACAGCAAT+AGG + chr8.4:1537776-1537795 MS.gene68875:CDS 45.0%
AGAGATTGAGGGCTGCGTTT+GGG + chr8.4:1538010-1538029 MS.gene68875:CDS 50.0%
AGCAAAGCCCGGACAAATTG+CGG - chr8.4:1537880-1537899 None:intergenic 50.0%
AGGTTCAGCCGCAATTTGTC+CGG + chr8.4:1537869-1537888 MS.gene68875:CDS 50.0%
CACGGACCTAATCCAGCAAT+AGG - chr8.4:1538115-1538134 None:intergenic 50.0%
CGCCCAAAACACATCCTGAT+AGG + chr8.4:1537755-1537774 MS.gene68875:CDS 50.0%
GGTGGTAGAAGAAAGAAGGG+TGG + chr8.4:1537559-1537578 MS.gene68875:CDS 50.0%
GTGGCTCCTATTGCTGGATT+AGG + chr8.4:1538106-1538125 MS.gene68875:CDS 50.0%
!! CAACCCCACCATTTGGTGTT+TGG - chr8.4:1537615-1537634 None:intergenic 50.0%
AAGGGGGCAAATTCTGCCGT+TGG + chr8.4:1538160-1538179 MS.gene68875:CDS 55.0%
AGGGGGCAAATTCTGCCGTT+GGG + chr8.4:1538161-1538180 MS.gene68875:CDS 55.0%
CACAGCAGGATCTCCACAGT+CGG + chr8.4:1538046-1538065 MS.gene68875:CDS 55.0%
CAGAGATTGAGGGCTGCGTT+TGG + chr8.4:1538009-1538028 MS.gene68875:CDS 55.0%
GGTTCAGCCGCAATTTGTCC+GGG + chr8.4:1537870-1537889 MS.gene68875:CDS 55.0%
TAGGTCCGTGTTTGCCAGCA+CGG + chr8.4:1538125-1538144 MS.gene68875:CDS 55.0%
TCCAGCAATAGGAGCCACCA+CGG - chr8.4:1538104-1538123 None:intergenic 55.0%
TGTCCGGGCTTTGCTTCGTA+GGG + chr8.4:1537885-1537904 MS.gene68875:CDS 55.0%
TTAGGTGCTTCTGCTGTCCG+TGG + chr8.4:1538084-1538103 MS.gene68875:CDS 55.0%
TTGTCCGGGCTTTGCTTCGT+AGG + chr8.4:1537884-1537903 MS.gene68875:CDS 55.0%
GAGCCCTACGAAGCAAAGCC+CGG - chr8.4:1537891-1537910 None:intergenic 60.0%
GGCTTTGCTTCGTAGGGCTC+GGG + chr8.4:1537891-1537910 MS.gene68875:CDS 60.0%
GGGCTTTGCTTCGTAGGGCT+CGG + chr8.4:1537890-1537909 MS.gene68875:CDS 60.0%
TCCGTGGTGGCTCCTATTGC+TGG + chr8.4:1538100-1538119 MS.gene68875:CDS 60.0%
TCCTGCTGTGCTTCCTGACG+AGG - chr8.4:1538036-1538055 None:intergenic 60.0%
TTTGCCCCCTTCTTCGACGC+AGG - chr8.4:1538151-1538170 None:intergenic 60.0%
!! GCAGAAGCACCTAACGCAGC+AGG - chr8.4:1538078-1538097 None:intergenic 60.0%
ACGGCCTGCGTCGAAGAAGG+GGG + chr8.4:1538144-1538163 MS.gene68875:CDS 65.0%
AGCACGGCCTGCGTCGAAGA+AGG + chr8.4:1538141-1538160 MS.gene68875:CDS 65.0%
CACGGCCTGCGTCGAAGAAG+GGG + chr8.4:1538143-1538162 MS.gene68875:CDS 65.0%
GCACCTAACGCAGCAGGCGA+AGG - chr8.4:1538072-1538091 None:intergenic 65.0%
GCACGGCCTGCGTCGAAGAA+GGG + chr8.4:1538142-1538161 MS.gene68875:CDS 65.0%
GGTGCTTCTGCTGTCCGTGG+TGG + chr8.4:1538087-1538106 MS.gene68875:CDS 65.0%
TCGTAGGGCTCGGGCGTTTG+AGG + chr8.4:1537900-1537919 MS.gene68875:CDS 65.0%
TTCTTCGACGCAGGCCGTGC+TGG - chr8.4:1538142-1538161 None:intergenic 65.0%
! GCAGGCCGTGCTGGCAAACA+CGG - chr8.4:1538133-1538152 None:intergenic 65.0%
! GCCTCGTCAGGAAGCACAGC+AGG + chr8.4:1538032-1538051 MS.gene68875:CDS 65.0%
! GCTCGGGCGTTTGAGGCTGT+TGG + chr8.4:1537907-1537926 MS.gene68875:CDS 65.0%
CAGCAGGCGAAGGCCGACTG+TGG - chr8.4:1538062-1538081 None:intergenic 70.0%
CGGCCTTCGCCTGCTGCGTT+AGG + chr8.4:1538066-1538085 MS.gene68875:CDS 70.0%
GGCTGCGTTTGGGCCTCGTC+AGG + chr8.4:1538020-1538039 MS.gene68875:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 1537547 1538200 1537547 ID=MS.gene68875
chr8.4 mRNA 1537547 1538200 1537547 ID=MS.gene68875.t1;Parent=MS.gene68875
chr8.4 exon 1537547 1538200 1537547 ID=MS.gene68875.t1.exon1;Parent=MS.gene68875.t1
chr8.4 CDS 1537547 1538200 1537547 ID=cds.MS.gene68875.t1;Parent=MS.gene68875.t1
Gene Sequence

>MS.gene68875

ATGGGGAAATCAGGTGGTAGAAGAAAGAAGGGTGGTGCTGTTGTTGACAATTCTTCAGCTTCTGTCCAAACACCAAATGGTGGGGTTGAATTGGATTCATCAATTTTCTTGAAGAAGGCACATGAAATGAAAGAAGAAGGGAATAGAAGGTTTCAGAGTAAGGATTATACTGGTGCTCTTGAAAACTATGAGAATGCTCTGAAACTCACGCCCAAAACACATCCTGATAGGGCTGTTTTTCACAGCAATAGGGCTGCTTGTATGATGCAAATGAAACCTATTGATTATGAATCTGTGATTTCTGAGTGTACTTTGGCTCTTCAGGTTCAGCCGCAATTTGTCCGGGCTTTGCTTCGTAGGGCTCGGGCGTTTGAGGCTGTTGGTAAGTATGAATTGGCTGTTCAAGATGTGCAGTTGTTATTGGCTTCTGATCCCAATCATAAGGATGCTTTGGATATTGCTCAGAGATTGAGGGCTGCGTTTGGGCCTCGTCAGGAAGCACAGCAGGATCTCCACAGTCGGCCTTCGCCTGCTGCGTTAGGTGCTTCTGCTGTCCGTGGTGGCTCCTATTGCTGGATTAGGTCCGTGTTTGCCAGCACGGCCTGCGTCGAAGAAGGGGGCAAATTCTGCCGTTGGGTCAGTTGTTTCTCCTAA

Protein sequence

>MS.gene68875.t1

MGKSGGRRKKGGAVVDNSSASVQTPNGGVELDSSIFLKKAHEMKEEGNRRFQSKDYTGALENYENALKLTPKTHPDRAVFHSNRAACMMQMKPIDYESVISECTLALQVQPQFVRALLRRARAFEAVGKYELAVQDVQLLLASDPNHKDALDIAQRLRAAFGPRQEAQQDLHSRPSPAALGASAVRGGSYCWIRSVFASTACVEEGGKFCRWVSCFS