Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69065.t1 | AFK44076.1 | 100 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 2.20E-22 | 114 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69065.t1 | O04173 | 71.4 | 56 | 13 | 1 | 1 | 56 | 1 | 53 | 1.7e-15 | 82.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69065.t1 | G7KSG1 | 100.0 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 1.6e-22 | 114.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052358 | MS.gene69065 | 0.805944 | 1.05E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene69065 | -0.803511 | 3.37E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene69065 | 0.844984 | 5.39E-59 | -1.69E-46 |
| MS.gene054468 | MS.gene69065 | -0.808764 | 2.64E-50 | -1.69E-46 |
| MS.gene056779 | MS.gene69065 | -0.812157 | 4.88E-51 | -1.69E-46 |
| MS.gene057026 | MS.gene69065 | -0.800812 | 1.21E-48 | -1.69E-46 |
| MS.gene060634 | MS.gene69065 | 0.808406 | 3.15E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene69065 | 0.825083 | 5.69E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69065.t1 | MTR_7g080740 | 100.000 | 56 | 0 | 0 | 1 | 56 | 1 | 56 | 2.26e-36 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69065.t1 | AT4G31720 | 71.429 | 56 | 13 | 1 | 1 | 56 | 1 | 53 | 1.80e-23 | 85.5 |
| MS.gene69065.t1 | AT4G31720 | 71.429 | 56 | 13 | 1 | 1 | 56 | 1 | 53 | 1.80e-23 | 85.5 |
| MS.gene69065.t1 | AT4G31720 | 71.429 | 56 | 13 | 1 | 1 | 56 | 1 | 53 | 1.80e-23 | 85.5 |
Find 14 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTTGATTGCGGCTGATTC+TGG | 0.178719 | 7.4:-25762906 | None:intergenic |
| TGAGTTGGTGGAGCATTACT+TGG | 0.429009 | 7.4:+25763099 | MS.gene69065:CDS |
| AACTAAGGAAGCTAGGAAAT+CGG | 0.431723 | 7.4:-25762965 | None:intergenic |
| TAATGCTCCACCAACTCATC+TGG | 0.444698 | 7.4:-25763094 | None:intergenic |
| TATAATCAACTAAGGAAGCT+AGG | 0.499784 | 7.4:-25762972 | None:intergenic |
| GGTACTCACAATCTCACATC+AGG | 0.516265 | 7.4:-25763142 | None:intergenic |
| CCTTCGCTTGAACTTGATTG+CGG | 0.520298 | 7.4:-25762916 | None:intergenic |
| AGGACATTGAAAGCCACTCT+TGG | 0.525406 | 7.4:-25763122 | None:intergenic |
| TTAGATACCAGATGAGTTGG+TGG | 0.530968 | 7.4:+25763087 | MS.gene69065:intron |
| AGTAGGAGTATAATCAACTA+AGG | 0.533067 | 7.4:-25762980 | None:intergenic |
| AATCAAGTTCAAGCGAAGGA+AGG | 0.545925 | 7.4:+25762920 | MS.gene69065:CDS |
| ATCAAGTTCAAGCGAAGGAA+GGG | 0.562141 | 7.4:+25762921 | MS.gene69065:CDS |
| GAGCATTACTTGGCCAAGAG+TGG | 0.569842 | 7.4:+25763109 | MS.gene69065:CDS |
| CCGCAATCAAGTTCAAGCGA+AGG | 0.622408 | 7.4:+25762916 | MS.gene69065:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATACATATCAGCATATAA+TGG | - | chr7.4:25763057-25763076 | None:intergenic | 20.0% |
| TATAATCAACTAAGGAAGCT+AGG | - | chr7.4:25762975-25762994 | None:intergenic | 30.0% | |
| ! | ACATATCAGCATATAATGGA+TGG | - | chr7.4:25763053-25763072 | None:intergenic | 30.0% |
| ! | AGTAGGAGTATAATCAACTA+AGG | - | chr7.4:25762983-25763002 | None:intergenic | 30.0% |
| AACTAAGGAAGCTAGGAAAT+CGG | - | chr7.4:25762968-25762987 | None:intergenic | 35.0% | |
| !! | GTTTTAGATACCAGATGAGT+TGG | + | chr7.4:25763084-25763103 | MS.gene69065:intron | 35.0% |
| AATCAAGTTCAAGCGAAGGA+AGG | + | chr7.4:25762920-25762939 | MS.gene69065:CDS | 40.0% | |
| ATCAAGTTCAAGCGAAGGAA+GGG | + | chr7.4:25762921-25762940 | MS.gene69065:CDS | 40.0% | |
| GTGAAAAGTGAACTGACAGT+AGG | - | chr7.4:25763000-25763019 | None:intergenic | 40.0% | |
| ! | TTAGATACCAGATGAGTTGG+TGG | + | chr7.4:25763087-25763106 | MS.gene69065:intron | 40.0% |
| AGGACATTGAAAGCCACTCT+TGG | - | chr7.4:25763125-25763144 | None:intergenic | 45.0% | |
| CCTTCGCTTGAACTTGATTG+CGG | - | chr7.4:25762919-25762938 | None:intergenic | 45.0% | |
| TAATGCTCCACCAACTCATC+TGG | - | chr7.4:25763097-25763116 | None:intergenic | 45.0% | |
| ! | AACTTGATTGCGGCTGATTC+TGG | - | chr7.4:25762909-25762928 | None:intergenic | 45.0% |
| !! | TGAGTTGGTGGAGCATTACT+TGG | + | chr7.4:25763099-25763118 | MS.gene69065:CDS | 45.0% |
| CCGCAATCAAGTTCAAGCGA+AGG | + | chr7.4:25762916-25762935 | MS.gene69065:CDS | 50.0% | |
| GAGCATTACTTGGCCAAGAG+TGG | + | chr7.4:25763109-25763128 | MS.gene69065:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 25762901 | 25763159 | 25762901 | ID=MS.gene69065 |
| chr7.4 | mRNA | 25762901 | 25763159 | 25762901 | ID=MS.gene69065.t1;Parent=MS.gene69065 |
| chr7.4 | exon | 25762901 | 25763002 | 25762901 | ID=MS.gene69065.t1.exon1;Parent=MS.gene69065.t1 |
| chr7.4 | CDS | 25762901 | 25763002 | 25762901 | ID=cds.MS.gene69065.t1;Parent=MS.gene69065.t1 |
| chr7.4 | exon | 25763091 | 25763159 | 25763091 | ID=MS.gene69065.t1.exon2;Parent=MS.gene69065.t1 |
| chr7.4 | CDS | 25763091 | 25763159 | 25763091 | ID=cds.MS.gene69065.t1;Parent=MS.gene69065.t1 |
| Gene Sequence |
| Protein sequence |
