AlfalfaGEDB | MS.gene69206

M. sativa cultivar XinJiangDaYe / MS.gene69206

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69206.t1	XP_013458006.1	96.8	126	4	0	1	126	1	126	2.40E-64	254.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69206.t1	I3SNP1	96.8	126	4	0	1	126	1	126	1.7e-64	254.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049385	MS.gene69206	0.83787	4.03E-57	-1.69E-46
MS.gene050077	MS.gene69206	0.802138	6.47E-49	-1.69E-46
MS.gene054291	MS.gene69206	0.806836	6.78E-50	-1.69E-46
MS.gene057156	MS.gene69206	0.820284	7.44E-53	-1.69E-46
MS.gene059338	MS.gene69206	0.829388	5.29E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69206.t1	MTR_4g109650	96.825	126	4	0	1	126	1	126	4.52e-88	251

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69206.t1	AT5G08060	53.846	130	55	2	1	125	1	130	1.27e-46	147

Find 47 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGATATCGTAAACGGTTTC+TGG	0.123041	8.3:-15060990	None:intergenic
ATCCTCTTCAACAATAATAT+TGG	0.235925	8.3:-15061138	None:intergenic
CTTGGTAGTGGCGGGGCAAT+AGG	0.256934	8.3:-15060940	None:intergenic
GGTAAGGCGGATTTGAATTC+GGG	0.330486	8.3:-15060897	None:intergenic
GGGTAAGGCGGATTTGAATT+CGG	0.340087	8.3:-15060898	None:intergenic
TTGAATTCGGGGTGGGAAAC+GGG	0.360855	8.3:-15060885	None:intergenic
TCCTCTTCAACAATAATATT+GGG	0.376182	8.3:-15061137	None:intergenic
TTTGAATTCGGGGTGGGAAA+CGG	0.389873	8.3:-15060886	None:intergenic
ACAATAATATTGGGATAAAT+CGG	0.396910	8.3:-15061128	None:intergenic
GATATCAGCTTTCTTGTAGA+TGG	0.398615	8.3:-15061060	None:intergenic
ATAGTATTTGATATCGTAAA+CGG	0.437783	8.3:-15060997	None:intergenic
TTGTAGATGGTGCGAACAAT+GGG	0.438576	8.3:-15061047	None:intergenic
GGCGGTGGTTACCAATCTTA+AGG	0.444855	8.3:+15061173	MS.gene69206:CDS
GACGGTTGCGGCGTTGATCA+CGG	0.445310	8.3:-15061022	None:intergenic
TTGGTAGTGGCGGGGCAATA+GGG	0.455000	8.3:-15060939	None:intergenic
AAAGCGCCATGGGAGATAAC+AGG	0.462207	8.3:+15060861	MS.gene69206:CDS
TCCCAATATTATTGTTGAAG+AGG	0.466589	8.3:+15061136	MS.gene69206:CDS
ACGGTTTCTGGATCGGAAGT+TGG	0.480049	8.3:-15060978	None:intergenic
ATCGTAAACGGTTTCTGGAT+CGG	0.484413	8.3:-15060985	None:intergenic
AGCTTCTGCAATAGGGAGGA+TGG	0.485294	8.3:-15060825	None:intergenic
CTTGTAGATGGTGCGAACAA+TGG	0.486997	8.3:-15061048	None:intergenic
ACGGTTGCGGCGTTGATCAC+GGG	0.489099	8.3:-15061021	None:intergenic
GAACAATGGGAGGACGGTTG+CGG	0.493599	8.3:-15061034	None:intergenic
GATATGAATGCTCGTGGCGG+TGG	0.519485	8.3:+15061158	MS.gene69206:CDS
GTAATCGAGTGCTCCGGGTA+AGG	0.522391	8.3:-15060913	None:intergenic
GGGACGGTAATCGAGTGCTC+CGG	0.539030	8.3:-15060919	None:intergenic
GGACGGTAATCGAGTGCTCC+GGG	0.539896	8.3:-15060918	None:intergenic
GCTTCTGCAATAGGGAGGAT+GGG	0.541038	8.3:-15060824	None:intergenic
GAATTCAAATCCGCCTTACC+CGG	0.542162	8.3:+15060900	MS.gene69206:CDS
ACGGGTCCTGTTATCTCCCA+TGG	0.545601	8.3:-15060867	None:intergenic
GGCGGATTTGAATTCGGGGT+GGG	0.546935	8.3:-15060892	None:intergenic
AAGCTGATATCGAGAAACTC+AGG	0.555463	8.3:+15061072	MS.gene69206:CDS
CTTCTGCAATAGGGAGGATG+GGG	0.560904	8.3:-15060823	None:intergenic
ATCGAGTGCTCCGGGTAAGG+CGG	0.581020	8.3:-15060910	None:intergenic
GGAGGATGGGGATTTCGCCA+TGG	0.597858	8.3:-15060811	None:intergenic
AAGCTTCATCAAAGCGCCAT+GGG	0.605317	8.3:+15060851	MS.gene69206:CDS
AAAGCTTCATCAAAGCGCCA+TGG	0.606824	8.3:+15060850	MS.gene69206:CDS
CTATTGCCCCGCCACTACCA+AGG	0.608087	8.3:+15060941	MS.gene69206:CDS
TGGTGCGAACAATGGGAGGA+CGG	0.610543	8.3:-15061040	None:intergenic
GTAAGGCGGATTTGAATTCG+GGG	0.621827	8.3:-15060896	None:intergenic
TTTCAGCTTCTGCAATAGGG+AGG	0.622425	8.3:-15060829	None:intergenic
TAGTGGCGGGGCAATAGGGA+CGG	0.630605	8.3:-15060935	None:intergenic
ATAATATTGGGATAAATCGG+AGG	0.646994	8.3:-15061125	None:intergenic
TAGATGGTGCGAACAATGGG+AGG	0.648838	8.3:-15061044	None:intergenic
AGGCGGATTTGAATTCGGGG+TGG	0.660754	8.3:-15060893	None:intergenic
GAAGAGGATATGAATGCTCG+TGG	0.716215	8.3:+15061152	MS.gene69206:CDS
GAGGATATGAATGCTCGTGG+CGG	0.770619	8.3:+15061155	MS.gene69206:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAATAATATTGGGATAAAT+CGG	-	chr8.3:15061131-15061150	None:intergenic	20.0%
!!	ATAGTATTTGATATCGTAAA+CGG	-	chr8.3:15061000-15061019	None:intergenic	20.0%
!	ATCCTCTTCAACAATAATAT+TGG	-	chr8.3:15061141-15061160	None:intergenic	25.0%
!	TCCTCTTCAACAATAATATT+GGG	-	chr8.3:15061140-15061159	None:intergenic	25.0%
	ATAATATTGGGATAAATCGG+AGG	-	chr8.3:15061128-15061147	None:intergenic	30.0%
	TCCCAATATTATTGTTGAAG+AGG	+	chr8.3:15061136-15061155	MS.gene69206:CDS	30.0%
	GATATCAGCTTTCTTGTAGA+TGG	-	chr8.3:15061063-15061082	None:intergenic	35.0%
	TTGATATCGTAAACGGTTTC+TGG	-	chr8.3:15060993-15061012	None:intergenic	35.0%
!!!	TGGAATAATCGCTTTTTCCT+TGG	-	chr8.3:15060961-15060980	None:intergenic	35.0%
	AAGCTGATATCGAGAAACTC+AGG	+	chr8.3:15061072-15061091	MS.gene69206:CDS	40.0%
	TTGTAGATGGTGCGAACAAT+GGG	-	chr8.3:15061050-15061069	None:intergenic	40.0%
!	AGCTTTTCAGCTTCTGCAAT+AGG	-	chr8.3:15060836-15060855	None:intergenic	40.0%
!	ATCGTAAACGGTTTCTGGAT+CGG	-	chr8.3:15060988-15061007	None:intergenic	40.0%
!	GCTTTTCAGCTTCTGCAATA+GGG	-	chr8.3:15060835-15060854	None:intergenic	40.0%
!!!	AATCGCTTTTTCCTTGGTAG+TGG	-	chr8.3:15060955-15060974	None:intergenic	40.0%
	CTTGTAGATGGTGCGAACAA+TGG	-	chr8.3:15061051-15061070	None:intergenic	45.0%
	GAAGAGGATATGAATGCTCG+TGG	+	chr8.3:15061152-15061171	MS.gene69206:CDS	45.0%
	GAATTCAAATCCGCCTTACC+CGG	+	chr8.3:15060900-15060919	MS.gene69206:CDS	45.0%
	TTTCAGCTTCTGCAATAGGG+AGG	-	chr8.3:15060832-15060851	None:intergenic	45.0%
	TTTGAATTCGGGGTGGGAAA+CGG	-	chr8.3:15060889-15060908	None:intergenic	45.0%
!	AAAGCTTCATCAAAGCGCCA+TGG	+	chr8.3:15060850-15060869	MS.gene69206:CDS	45.0%
!	AAGCTTCATCAAAGCGCCAT+GGG	+	chr8.3:15060851-15060870	MS.gene69206:CDS	45.0%
!	GGGTAAGGCGGATTTGAATT+CGG	-	chr8.3:15060901-15060920	None:intergenic	45.0%
!	GGTAAGGCGGATTTGAATTC+GGG	-	chr8.3:15060900-15060919	None:intergenic	45.0%
!	GTAAGGCGGATTTGAATTCG+GGG	-	chr8.3:15060899-15060918	None:intergenic	45.0%
	AGCTTCTGCAATAGGGAGGA+TGG	-	chr8.3:15060828-15060847	None:intergenic	50.0%
	CTTCTGCAATAGGGAGGATG+GGG	-	chr8.3:15060826-15060845	None:intergenic	50.0%
	GAGGATATGAATGCTCGTGG+CGG	+	chr8.3:15061155-15061174	MS.gene69206:CDS	50.0%
	GCTTCTGCAATAGGGAGGAT+GGG	-	chr8.3:15060827-15060846	None:intergenic	50.0%
	TAGATGGTGCGAACAATGGG+AGG	-	chr8.3:15061047-15061066	None:intergenic	50.0%
	TTGAATTCGGGGTGGGAAAC+GGG	-	chr8.3:15060888-15060907	None:intergenic	50.0%
!	AAAGCGCCATGGGAGATAAC+AGG	+	chr8.3:15060861-15060880	MS.gene69206:CDS	50.0%
!	ACGGTTTCTGGATCGGAAGT+TGG	-	chr8.3:15060981-15061000	None:intergenic	50.0%
!!!	CGCTTTTTCCTTGGTAGTGG+CGG	-	chr8.3:15060952-15060971	None:intergenic	50.0%
!!!	CTTTTTCCTTGGTAGTGGCG+GGG	-	chr8.3:15060950-15060969	None:intergenic	50.0%
!!!	GCTTTTTCCTTGGTAGTGGC+GGG	-	chr8.3:15060951-15060970	None:intergenic	50.0%
	ACGGGTCCTGTTATCTCCCA+TGG	-	chr8.3:15060870-15060889	None:intergenic	55.0%
	GAACAATGGGAGGACGGTTG+CGG	-	chr8.3:15061037-15061056	None:intergenic	55.0%
	GATATGAATGCTCGTGGCGG+TGG	+	chr8.3:15061158-15061177	MS.gene69206:CDS	55.0%
	GTAATCGAGTGCTCCGGGTA+AGG	-	chr8.3:15060916-15060935	None:intergenic	55.0%
	TGGTGCGAACAATGGGAGGA+CGG	-	chr8.3:15061043-15061062	None:intergenic	55.0%
!	AGGCGGATTTGAATTCGGGG+TGG	-	chr8.3:15060896-15060915	None:intergenic	55.0%
!	GGCGGATTTGAATTCGGGGT+GGG	-	chr8.3:15060895-15060914	None:intergenic	55.0%
!!	TTGGTAGTGGCGGGGCAATA+GGG	-	chr8.3:15060942-15060961	None:intergenic	55.0%
	ATCGAGTGCTCCGGGTAAGG+CGG	-	chr8.3:15060913-15060932	None:intergenic	60.0%
	CTATTGCCCCGCCACTACCA+AGG	+	chr8.3:15060941-15060960	MS.gene69206:CDS	60.0%
	GGACGGTAATCGAGTGCTCC+GGG	-	chr8.3:15060921-15060940	None:intergenic	60.0%
	GGGACGGTAATCGAGTGCTC+CGG	-	chr8.3:15060922-15060941	None:intergenic	60.0%
!	TAGTGGCGGGGCAATAGGGA+CGG	-	chr8.3:15060938-15060957	None:intergenic	60.0%
!!	ACGGTTGCGGCGTTGATCAC+GGG	-	chr8.3:15061024-15061043	None:intergenic	60.0%
!!	CTTGGTAGTGGCGGGGCAAT+AGG	-	chr8.3:15060943-15060962	None:intergenic	60.0%
!!	GACGGTTGCGGCGTTGATCA+CGG	-	chr8.3:15061025-15061044	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	15060813	15061193	15060813	ID=MS.gene69206
chr8.3	mRNA	15060813	15061193	15060813	ID=MS.gene69206.t1;Parent=MS.gene69206
chr8.3	exon	15060813	15061193	15060813	ID=MS.gene69206.t1.exon1;Parent=MS.gene69206.t1
chr8.3	CDS	15060813	15061193	15060813	ID=cds.MS.gene69206.t1;Parent=MS.gene69206.t1

Gene Sequence

>MS.gene69206

ATGGCGAAATCCCCATCCTCCCTATTGCAGAAGCTGAAAAGCTTCATCAAAGCGCCATGGGAGATAACAGGACCCGTTTCCCACCCCGAATTCAAATCCGCCTTACCCGGAGCACTCGATTACCGTCCCTATTGCCCCGCCACTACCAAGGAAAAAGCGATTATTCCAACTTCCGATCCAGAAACCGTTTACGATATCAAATACTATACCCGTGATCAACGCCGCAACCGTCCTCCCATTGTTCGCACCATCTACAAGAAAGCTGATATCGAGAAACTCAGGAAAGAAGCTACTTATGACGTCGAAGATTTTCCTCCGATTTATCCCAATATTATTGTTGAAGAGGATATGAATGCTCGTGGCGGTGGTTACCAATCTTAA

Protein sequence

>MS.gene69206.t1

MAKSPSSLLQKLKSFIKAPWEITGPVSHPEFKSALPGALDYRPYCPATTKEKAIIPTSDPETVYDIKYYTRDQRRNRPPIVRTIYKKADIEKLRKEATYDVEDFPPIYPNIIVEEDMNARGGGYQS