AlfalfaGEDB | MS.gene70827

M. sativa cultivar XinJiangDaYe / MS.gene70827

KEGG: -
GO: GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0042742 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542
PFAM: PF00967.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70827.t1	XP_013468974.1	95.8	144	6	0	1	144	1	144	2.30E-75	291.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70827.t1	P29062	71.7	138	37	1	8	145	11	146	2.1e-56	219.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70827.t1	A0A072VN05	95.8	144	6	0	1	144	1	144	1.7e-75	291.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049993	MS.gene70827	0.826061	3.34E-54	-1.69E-46
MS.gene051980	MS.gene70827	0.813322	2.71E-51	-1.69E-46
MS.gene052011	MS.gene70827	-0.801035	1.09E-48	-1.69E-46
MS.gene053873	MS.gene70827	0.821414	4.09E-53	-1.69E-46
MS.gene054102	MS.gene70827	0.801864	7.36E-49	-1.69E-46
MS.gene054108	MS.gene70827	0.800233	1.59E-48	-1.69E-46
MS.gene054183	MS.gene70827	0.809877	1.52E-50	-1.69E-46
MS.gene056514	MS.gene70827	0.825384	4.83E-54	-1.69E-46
MS.gene056981	MS.gene70827	0.83462	2.70E-56	-1.69E-46
MS.gene057476	MS.gene70827	0.831539	1.58E-55	-1.69E-46
MS.gene057841	MS.gene70827	0.8119	5.55E-51	-1.69E-46
MS.gene057888	MS.gene70827	0.822901	1.85E-53	-1.69E-46
MS.gene061214	MS.gene70827	0.801495	8.77E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70827.t1	MTR_1g080800	95.833	144	6	0	1	144	1	144	1.00e-99	283
MS.gene70827.t1	MTR_1g080820	90.278	144	14	0	1	144	1	144	1.55e-93	268
MS.gene70827.t1	MTR_1g080810	84.722	144	22	0	1	144	1	144	1.57e-89	258
MS.gene70827.t1	MTR_7g115220	70.000	120	36	0	29	148	69	188	1.63e-59	184
MS.gene70827.t1	MTR_1g080780	59.124	137	55	1	8	144	4	139	5.38e-57	175
MS.gene70827.t1	MTR_6g083270	52.866	157	53	5	10	149	11	163	1.16e-46	158
MS.gene70827.t1	MTR_6g083200	51.799	139	60	3	9	145	10	143	1.91e-45	148
MS.gene70827.t1	MTR_6g083290	46.479	142	62	3	10	144	11	145	1.89e-38	133
MS.gene70827.t1	MTR_6g083310	49.333	150	56	5	10	144	11	155	2.15e-38	129

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70827.t1	AT3G04720	65.468	139	48	0	24	162	71	209	2.69e-62	191

Find 40 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTCATGGCGTAGCAAATA+TGG	0.308099	1.2:+61359425	MS.gene70827:CDS
TCTCTGCCTGTTGGCCCAGC+TGG	0.347389	1.2:-61359467	None:intergenic
ACAGAGAACTTTGAGCTTGT+TGG	0.365729	1.2:+61359253	MS.gene70827:CDS
ACAACCCTCAGAATATTAAC+TGG	0.383186	1.2:+61359351	MS.gene70827:CDS
GTCAGAGTGCAAACAACGTT+AGG	0.437077	1.2:+61359312	MS.gene70827:CDS
TGTGGACCAGCTGGGCCAAC+AGG	0.437465	1.2:+61359461	MS.gene70827:CDS
CCAGTGTATACTGTGCTACT+TGG	0.451011	1.2:+61359387	MS.gene70827:CDS
TAACTGTAAGGTGACCCTTC+TGG	0.462881	1.2:-61359890	None:intergenic
TCAAATTGATACCAATGGAC+AGG	0.479120	1.2:+61359863	MS.gene70827:CDS
CAAAGCTATAGTTAACTGTA+AGG	0.486461	1.2:-61359902	None:intergenic
GAATACTGCAACTGGTGCTC+AGG	0.493434	1.2:+61359776	MS.gene70827:CDS
TGCTACGCCATGACAAGGGC+TGG	0.498360	1.2:-61359418	None:intergenic
ATAGTGGACCAGTGCTCAAA+TGG	0.501061	1.2:+61359810	MS.gene70827:CDS
CAGGTGACGAATACTGCAAC+TGG	0.524837	1.2:+61359768	MS.gene70827:intron
CCGCAAGAATCTCTGCCTGT+TGG	0.525418	1.2:-61359476	None:intergenic
CAGTGTATACTGTGCTACTT+GGG	0.526139	1.2:+61359388	MS.gene70827:CDS
TATAGCTTTGTTAATTGCAA+CGG	0.526692	1.2:+61359915	MS.gene70827:CDS
GTGATTTATTGACTCATCAC+TGG	0.540907	1.2:+61359937	MS.gene70827:CDS
TTCAATCAAATTGATACCAA+TGG	0.541175	1.2:+61359858	MS.gene70827:CDS
CCAGTGCTCAAATGGTGGAC+TGG	0.542110	1.2:+61359818	MS.gene70827:CDS
CTTCTGGTAACCCTGTCCAT+TGG	0.545362	1.2:-61359874	None:intergenic
CCAGTCCACCATTTGAGCAC+TGG	0.554865	1.2:-61359818	None:intergenic
TAATCCCAGTTAATATTCTG+AGG	0.556889	1.2:-61359356	None:intergenic
AATGGACAGGGTTACCAGAA+GGG	0.563174	1.2:+61359876	MS.gene70827:CDS
TGCAACTGGTGCTCAGGTAA+CGG	0.577038	1.2:+61359782	MS.gene70827:CDS
TCAGAGTGCAAACAACGTTA+GGG	0.580216	1.2:+61359313	MS.gene70827:CDS
CAACCCTCAGAATATTAACT+GGG	0.582070	1.2:+61359352	MS.gene70827:CDS
ATGCAAACCAGCCCTTGTCA+TGG	0.583448	1.2:+61359411	MS.gene70827:CDS
ATATTTGCTACGCCATGACA+AGG	0.584472	1.2:-61359423	None:intergenic
CAAATTGATACCAATGGACA+GGG	0.591064	1.2:+61359864	MS.gene70827:CDS
CATGGCGTAGCAAATATGGT+TGG	0.604474	1.2:+61359429	MS.gene70827:CDS
TCAGGTAACGGTGAGAATAG+TGG	0.610066	1.2:+61359794	MS.gene70827:CDS
CAATGGACAGGGTTACCAGA+AGG	0.617429	1.2:+61359875	MS.gene70827:CDS
GTGGACCAGTGCTCAAATGG+TGG	0.625188	1.2:+61359813	MS.gene70827:CDS
AATCCCAGTTAATATTCTGA+GGG	0.629321	1.2:-61359355	None:intergenic
CCAAGTAGCACAGTATACAC+TGG	0.657659	1.2:-61359387	None:intergenic
TCACTAGCATCGTTCCCACA+AGG	0.659432	1.2:-61359286	None:intergenic
TATTTGCTACGCCATGACAA+GGG	0.664421	1.2:-61359422	None:intergenic
CCAACAGGCAGAGATTCTTG+CGG	0.667208	1.2:+61359476	MS.gene70827:CDS
GTGGGAACGATGCTAGTGAG+TGG	0.745826	1.2:+61359290	MS.gene70827:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTCAATTAATGATAGTATTG+TGG	+	chr1.2:61359730-61359749	MS.gene70827:intron	20.0%
!	TTCAATCAAATTGATACCAA+TGG	+	chr1.2:61359858-61359877	MS.gene70827:CDS	25.0%
!!	ACTCACTAATTTCTTCATTT+TGG	+	chr1.2:61359615-61359634	MS.gene70827:intron	25.0%
!!	CTCACTAATTTCTTCATTTT+GGG	+	chr1.2:61359616-61359635	MS.gene70827:intron	25.0%
!!	TCACTAATTTCTTCATTTTG+GGG	+	chr1.2:61359617-61359636	MS.gene70827:intron	25.0%
!!!	TATAGCTTTGTTAATTGCAA+CGG	+	chr1.2:61359915-61359934	MS.gene70827:CDS	25.0%
!!!	TTTTTCTAAAATGAGAGAAG+TGG	-	chr1.2:61359521-61359540	None:intergenic	25.0%
	AATCCCAGTTAATATTCTGA+GGG	-	chr1.2:61359358-61359377	None:intergenic	30.0%
	AGAAATTAGTGAGTTTGTGT+TGG	-	chr1.2:61359609-61359628	None:intergenic	30.0%
	CAAAGCTATAGTTAACTGTA+AGG	-	chr1.2:61359905-61359924	None:intergenic	30.0%
	TAATCCCAGTTAATATTCTG+AGG	-	chr1.2:61359359-61359378	None:intergenic	30.0%
!	ATTCTCTTTGACTAGCTAAT+TGG	-	chr1.2:61359691-61359710	None:intergenic	30.0%
	ACAACCCTCAGAATATTAAC+TGG	+	chr1.2:61359351-61359370	MS.gene70827:CDS	35.0%
	CAAATTGATACCAATGGACA+GGG	+	chr1.2:61359864-61359883	MS.gene70827:CDS	35.0%
	CAACCCTCAGAATATTAACT+GGG	+	chr1.2:61359352-61359371	MS.gene70827:CDS	35.0%
	TAGCTAGTCAAAGAGAATTG+TGG	+	chr1.2:61359693-61359712	MS.gene70827:intron	35.0%
	TCAAATTGATACCAATGGAC+AGG	+	chr1.2:61359863-61359882	MS.gene70827:CDS	35.0%
!!	GTGATTTATTGACTCATCAC+TGG	+	chr1.2:61359937-61359956	MS.gene70827:CDS	35.0%
!!	TGTGTTGGTCAGATTTTAGA+GGG	-	chr1.2:61359594-61359613	None:intergenic	35.0%
!!	TTGTGTTGGTCAGATTTTAG+AGG	-	chr1.2:61359595-61359614	None:intergenic	35.0%
!!!	TTGGTTTTGTGTTTCCTTGT+GGG	+	chr1.2:61359272-61359291	MS.gene70827:CDS	35.0%
	ACAGAGAACTTTGAGCTTGT+TGG	+	chr1.2:61359253-61359272	MS.gene70827:CDS	40.0%
	TCAGAGTGCAAACAACGTTA+GGG	+	chr1.2:61359313-61359332	MS.gene70827:CDS	40.0%
	TTGTCATGGCGTAGCAAATA+TGG	+	chr1.2:61359425-61359444	MS.gene70827:CDS	40.0%
!	ATATTTGCTACGCCATGACA+AGG	-	chr1.2:61359426-61359445	None:intergenic	40.0%
!	TATTTGCTACGCCATGACAA+GGG	-	chr1.2:61359425-61359444	None:intergenic	40.0%
!!	CAGTGTATACTGTGCTACTT+GGG	+	chr1.2:61359388-61359407	MS.gene70827:CDS	40.0%
!!!	GTTGGTTTTGTGTTTCCTTG+TGG	+	chr1.2:61359271-61359290	MS.gene70827:CDS	40.0%
!!!	TATGGTTGGACTGCTTTTTG+TGG	+	chr1.2:61359443-61359462	MS.gene70827:CDS	40.0%
	AATGGACAGGGTTACCAGAA+GGG	+	chr1.2:61359876-61359895	MS.gene70827:CDS	45.0%
	CATGGCGTAGCAAATATGGT+TGG	+	chr1.2:61359429-61359448	MS.gene70827:CDS	45.0%
	CCAAGTAGCACAGTATACAC+TGG	-	chr1.2:61359390-61359409	None:intergenic	45.0%
	GTATTCGTCACCTGCATCAT+CGG	-	chr1.2:61359762-61359781	None:intergenic	45.0%
	GTCAGAGTGCAAACAACGTT+AGG	+	chr1.2:61359312-61359331	MS.gene70827:CDS	45.0%
	TAACTGTAAGGTGACCCTTC+TGG	-	chr1.2:61359893-61359912	None:intergenic	45.0%
!	CCAGTGTATACTGTGCTACT+TGG	+	chr1.2:61359387-61359406	MS.gene70827:CDS	45.0%
!	TCAGGTAACGGTGAGAATAG+TGG	+	chr1.2:61359794-61359813	MS.gene70827:CDS	45.0%
!!	ATAGTGGACCAGTGCTCAAA+TGG	+	chr1.2:61359810-61359829	MS.gene70827:CDS	45.0%
	ATGCAAACCAGCCCTTGTCA+TGG	+	chr1.2:61359411-61359430	MS.gene70827:CDS	50.0%
	CAATGGACAGGGTTACCAGA+AGG	+	chr1.2:61359875-61359894	MS.gene70827:CDS	50.0%
	CAGGTGACGAATACTGCAAC+TGG	+	chr1.2:61359768-61359787	MS.gene70827:intron	50.0%
	CCAACAGGCAGAGATTCTTG+CGG	+	chr1.2:61359476-61359495	MS.gene70827:CDS	50.0%
	CTTCTGGTAACCCTGTCCAT+TGG	-	chr1.2:61359877-61359896	None:intergenic	50.0%
	GAATACTGCAACTGGTGCTC+AGG	+	chr1.2:61359776-61359795	MS.gene70827:CDS	50.0%
	TCACTAGCATCGTTCCCACA+AGG	-	chr1.2:61359289-61359308	None:intergenic	50.0%
	TGCAACTGGTGCTCAGGTAA+CGG	+	chr1.2:61359782-61359801	MS.gene70827:CDS	50.0%
!!!	ACTGCTTTTTGTGGACCAGC+TGG	+	chr1.2:61359452-61359471	MS.gene70827:CDS	50.0%
!!!	CTGCTTTTTGTGGACCAGCT+GGG	+	chr1.2:61359453-61359472	MS.gene70827:CDS	50.0%
	CCAGTCCACCATTTGAGCAC+TGG	-	chr1.2:61359821-61359840	None:intergenic	55.0%
	CCGCAAGAATCTCTGCCTGT+TGG	-	chr1.2:61359479-61359498	None:intergenic	55.0%
	GTGGACCAGTGCTCAAATGG+TGG	+	chr1.2:61359813-61359832	MS.gene70827:CDS	55.0%
	GTGGGAACGATGCTAGTGAG+TGG	+	chr1.2:61359290-61359309	MS.gene70827:CDS	55.0%
!	GTGGTGATGACCGATGATGC+AGG	+	chr1.2:61359749-61359768	MS.gene70827:intron	55.0%
!!	CCAGTGCTCAAATGGTGGAC+TGG	+	chr1.2:61359818-61359837	MS.gene70827:CDS	55.0%
	TGCTACGCCATGACAAGGGC+TGG	-	chr1.2:61359421-61359440	None:intergenic	60.0%
	TCTCTGCCTGTTGGCCCAGC+TGG	-	chr1.2:61359470-61359489	None:intergenic	65.0%
	TGTGGACCAGCTGGGCCAAC+AGG	+	chr1.2:61359461-61359480	MS.gene70827:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	61359242	61359995	61359242	ID=MS.gene70827
chr1.2	mRNA	61359242	61359995	61359242	ID=MS.gene70827.t1;Parent=MS.gene70827
chr1.2	exon	61359242	61359511	61359242	ID=MS.gene70827.t1.exon1;Parent=MS.gene70827.t1
chr1.2	CDS	61359242	61359511	61359242	ID=cds.MS.gene70827.t1;Parent=MS.gene70827.t1
chr1.2	exon	61359771	61359995	61359771	ID=MS.gene70827.t1.exon2;Parent=MS.gene70827.t1
chr1.2	CDS	61359771	61359995	61359771	ID=cds.MS.gene70827.t1;Parent=MS.gene70827.t1

Gene Sequence

>MS.gene70827

ATGGAGAGCACACAGAGAACTTTGAGCTTGTTGGTTTTGTGTTTCCTTGTGGGAACGATGCTAGTGAGTGGTCAGAGTGCAAACAACGTTAGGGCAACATACAACAATTACAACCCTCAGAATATTAACTGGGATTACATTACTGCCAGTGTATACTGTGCTACTTGGGATGCAAACCAGCCCTTGTCATGGCGTAGCAAATATGGTTGGACTGCTTTTTGTGGACCAGCTGGGCCAACAGGCAGAGATTCTTGCGGCAAATGCTTGAGTGTATGTCCACTTCTCTCATTTTAGAAAAATATTAAATGTACATCATTTTGAACTTAATTCAATTTTTACTGAAATTAATCCCTCTAAAATCTGACCAACACAAACTCACTAATTTCTTCATTTTGGGGCATTTTTTGATTTAGTAGTTGTTGTCATGTGCATGAACTTAATTATTGCCAATTAGCTAGTCAAAGAGAATTGTGGATTACTTGTTAAAGTTCAATTAATGATAGTATTGTGGTGATGACCGATGATGCAGGTGACGAATACTGCAACTGGTGCTCAGGTAACGGTGAGAATAGTGGACCAGTGCTCAAATGGTGGACTGGACTTAGATGTGAATGTCTTCAATCAAATTGATACCAATGGACAGGGTTACCAGAAGGGTCACCTTACAGTTAACTATAGCTTTGTTAATTGCAACGGTGATTTATTGACTCATCACTGGTGTAATAATTCACTCTTTGTACAAATAAGTAGCTAG

Protein sequence

>MS.gene70827.t1

MESTQRTLSLLVLCFLVGTMLVSGQSANNVRATYNNYNPQNINWDYITASVYCATWDANQPLSWRSKYGWTAFCGPAGPTGRDSCGKCLSVTNTATGAQVTVRIVDQCSNGGLDLDVNVFNQIDTNGQGYQKGHLTVNYSFVNCNGDLLTHHWCNNSLFVQISS