Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70845.t1 | AET00171.1 | 91.9 | 397 | 29 | 2 | 1 | 395 | 1 | 396 | 2.00E-205 | 724.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70845.t1 | G7KCV0 | 91.9 | 397 | 29 | 2 | 1 | 395 | 1 | 396 | 1.4e-205 | 724.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene70845 | 0.801787 | 7.63E-49 | -1.69E-46 |
| MS.gene049647 | MS.gene70845 | 0.818809 | 1.61E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene70845 | 0.807969 | 3.90E-50 | -1.69E-46 |
| MS.gene055608 | MS.gene70845 | 0.86397 | 1.70E-64 | -1.69E-46 |
| MS.gene056908 | MS.gene70845 | 0.807818 | 4.20E-50 | -1.69E-46 |
| MS.gene058146 | MS.gene70845 | 0.80522 | 1.48E-49 | -1.69E-46 |
| MS.gene060998 | MS.gene70845 | 0.831485 | 1.63E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70845.t1 | MTR_5g089100 | 91.940 | 397 | 29 | 2 | 1 | 395 | 1 | 396 | 0.0 | 724 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 68 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAACAAAAGAGGTGGATTT+TGG | 0.178214 | 5.3:+71917883 | MS.gene70845:CDS |
| CTTTCTGATTCTTCTTTCAT+TGG | 0.206679 | 5.3:-71917726 | None:intergenic |
| TGAGGATTCATTGCCTACTA+TGG | 0.314743 | 5.3:+71917287 | MS.gene70845:CDS |
| CCTTCTGGAAAAGACATTAA+AGG | 0.322160 | 5.3:-71917675 | None:intergenic |
| TCGGTTGTTACATGAGCTTC+TGG | 0.322517 | 5.3:+71917698 | MS.gene70845:CDS |
| TCTTGCAAACGCCAACCCTT+TGG | 0.323714 | 5.3:-71917769 | None:intergenic |
| TTTCGGTTTGTCTTAGTTCT+CGG | 0.336891 | 5.3:-71917228 | None:intergenic |
| CTTGCAAACGCCAACCCTTT+GGG | 0.352903 | 5.3:-71917768 | None:intergenic |
| TCATGTAACAACCGACCTTC+TGG | 0.353465 | 5.3:-71917690 | None:intergenic |
| AGAATTAGAGAAGCACTCTT+TGG | 0.357367 | 5.3:+71918004 | MS.gene70845:CDS |
| CCTCCAAGACTAGTTCAAAA+TGG | 0.363341 | 5.3:+71917372 | MS.gene70845:CDS |
| AGTTTAAGAGGCGGCATCAT+TGG | 0.387438 | 5.3:-71917348 | None:intergenic |
| TATGATGATCTTGGTGTTGT+AGG | 0.396948 | 5.3:-71917138 | None:intergenic |
| AAATTCAAACCGATCTAGAT+TGG | 0.397332 | 5.3:-71917175 | None:intergenic |
| GCATCTTCATATGATGATCT+TGG | 0.413582 | 5.3:-71917147 | None:intergenic |
| GAATGCACAGATTTCATGTC+AGG | 0.415124 | 5.3:-71918035 | None:intergenic |
| AGGGTTGGCGTTTGCAAGAC+AGG | 0.418597 | 5.3:+71917773 | MS.gene70845:CDS |
| TTCTCAAAAGCCACCTTGAA+TGG | 0.424398 | 5.3:-71917492 | None:intergenic |
| AGGTAAGAGAAGAGAAGAAA+GGG | 0.440659 | 5.3:+71917514 | MS.gene70845:CDS |
| TGGAGTAAAGATGATATATC+AGG | 0.442029 | 5.3:+71918074 | MS.gene70845:CDS |
| TTCTTCCACAAGCGTGCAAA+AGG | 0.442067 | 5.3:-71917456 | None:intergenic |
| TGAGTTTACTAAAATGAGAT+TGG | 0.445183 | 5.3:+71918097 | MS.gene70845:CDS |
| GAACACAGTGGTTATCAAAC+AGG | 0.451484 | 5.3:+71917114 | MS.gene70845:CDS |
| TTTCTTTGCCTGGGTTATGA+GGG | 0.465352 | 5.3:+71918146 | MS.gene70845:CDS |
| GAGAAGAAAGGGAAATCAAA+GGG | 0.466521 | 5.3:+71917525 | MS.gene70845:CDS |
| TCATCAGCAAATGCCATAGT+AGG | 0.468021 | 5.3:-71917300 | None:intergenic |
| TTGAGTCTGCTAGGACTAGT+TGG | 0.469582 | 5.3:-71917084 | None:intergenic |
| AGTCTTGGAGGAAGTTTAAG+AGG | 0.473364 | 5.3:-71917360 | None:intergenic |
| TTTGGTTTCTAATGTTGCTA+AGG | 0.475219 | 5.3:+71917845 | MS.gene70845:CDS |
| AGAGAAGAAAGGGAAATCAA+AGG | 0.475466 | 5.3:+71917524 | MS.gene70845:CDS |
| GTTTGAGGCTAAGAAGACTT+TGG | 0.477506 | 5.3:+71917827 | MS.gene70845:CDS |
| TAGATCCCTTTCTCCATTGA+GGG | 0.480062 | 5.3:+71917572 | MS.gene70845:CDS |
| TCTATTAATTTGAGTCTGCT+AGG | 0.481735 | 5.3:-71917093 | None:intergenic |
| AAGGTAAGAGAAGAGAAGAA+AGG | 0.483464 | 5.3:+71917513 | MS.gene70845:CDS |
| AGCATTGATCCTGGAGACTT+AGG | 0.489838 | 5.3:-71917426 | None:intergenic |
| TTGTTGTTGAAGTCCCTCAA+TGG | 0.492128 | 5.3:-71917585 | None:intergenic |
| GGCTAATGATACAAAGTTTG+AGG | 0.498660 | 5.3:+71917812 | MS.gene70845:CDS |
| TTTGCCTGGGTTATGAGGGA+TGG | 0.505640 | 5.3:+71918150 | MS.gene70845:CDS |
| AGAATCAGAAAGAGAAAGTA+CGG | 0.508154 | 5.3:+71917737 | MS.gene70845:CDS |
| TTTCTTTGGGAGTTCAAACA+TGG | 0.511688 | 5.3:+71917941 | MS.gene70845:CDS |
| CTAGATCCCTTTCTCCATTG+AGG | 0.518421 | 5.3:+71917571 | MS.gene70845:CDS |
| GGAAGCCTAAGCATTGATCC+TGG | 0.520514 | 5.3:-71917435 | None:intergenic |
| GAAGTCCCTCAATGGAGAAA+GGG | 0.528545 | 5.3:-71917577 | None:intergenic |
| AGTCTCCAGGATCAATGCTT+AGG | 0.529586 | 5.3:+71917430 | MS.gene70845:CDS |
| TGAAGTCCCTCAATGGAGAA+AGG | 0.529754 | 5.3:-71917578 | None:intergenic |
| ACAGGTGAGACAAGTGGAAG+TGG | 0.533037 | 5.3:+71917791 | MS.gene70845:CDS |
| CCCAGGCAAAGAAAAGACTT+TGG | 0.534703 | 5.3:-71918137 | None:intergenic |
| GTAGAGAGCATAGTTTAAGA+AGG | 0.548216 | 5.3:+71917547 | MS.gene70845:CDS |
| ATGCATTTGAGCAGCATCAA+AGG | 0.548678 | 5.3:+71917259 | MS.gene70845:CDS |
| GCGACGTCTCCTAAGTCTCC+AGG | 0.561265 | 5.3:+71917417 | MS.gene70845:CDS |
| AAAGATGAGAACAAAAGAGG+TGG | 0.561338 | 5.3:+71917876 | MS.gene70845:CDS |
| AAGAAAGATGAGAACAAAAG+AGG | 0.562093 | 5.3:+71917873 | MS.gene70845:CDS |
| AAATGAGATTGGTATGTCAA+AGG | 0.580349 | 5.3:+71918108 | MS.gene70845:CDS |
| ATGCAAACACCAATCTAGAT+CGG | 0.598062 | 5.3:+71917166 | MS.gene70845:CDS |
| CAAAGAAAAGACTTTGGCAA+TGG | 0.599126 | 5.3:-71918131 | None:intergenic |
| GTACGGTATCTGAGCCCAAA+GGG | 0.602552 | 5.3:+71917754 | MS.gene70845:CDS |
| CAACAAGTGTGACAAGCATG+AGG | 0.614324 | 5.3:+71917602 | MS.gene70845:CDS |
| GCAGCATCAAAGGCTTTGTG+AGG | 0.620784 | 5.3:+71917269 | MS.gene70845:CDS |
| GGTATCTGAGCCCAAAGGGT+TGG | 0.621729 | 5.3:+71917758 | MS.gene70845:CDS |
| CTTTCCATCCCTCATAACCC+AGG | 0.625041 | 5.3:-71918154 | None:intergenic |
| TTCTTTGGGAGTTCAAACAT+GGG | 0.630100 | 5.3:+71917942 | MS.gene70845:CDS |
| AGTACGGTATCTGAGCCCAA+AGG | 0.630219 | 5.3:+71917753 | MS.gene70845:CDS |
| TGCAAGACAGGTGAGACAAG+TGG | 0.641832 | 5.3:+71917785 | MS.gene70845:CDS |
| CTTGGAGGAAGTTTAAGAGG+CGG | 0.649976 | 5.3:-71917357 | None:intergenic |
| ACTCATGTAACATAACTCGT+TGG | 0.670365 | 5.3:-71917016 | None:intergenic |
| AACAAGTGTGACAAGCATGA+GGG | 0.672587 | 5.3:+71917603 | MS.gene70845:CDS |
| ATTCAACAGAATCAACTACA+TGG | 0.676364 | 5.3:+71918054 | MS.gene70845:CDS |
| CTCAAATTAATAGAACACAG+TGG | 0.795850 | 5.3:+71917102 | MS.gene70845:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTATAAAGAAGTTTTTCTT+TGG | + | chr5.3:71917927-71917946 | MS.gene70845:CDS | 20.0% |
| !!! | GTATAAAGAAGTTTTTCTTT+GGG | + | chr5.3:71917928-71917947 | MS.gene70845:CDS | 20.0% |
| ! | TGAGTTTACTAAAATGAGAT+TGG | + | chr5.3:71918097-71918116 | MS.gene70845:CDS | 25.0% |
| AAATGAGATTGGTATGTCAA+AGG | + | chr5.3:71918108-71918127 | MS.gene70845:CDS | 30.0% | |
| AAATTCAAACCGATCTAGAT+TGG | - | chr5.3:71917178-71917197 | None:intergenic | 30.0% | |
| AAGAAAGATGAGAACAAAAG+AGG | + | chr5.3:71917873-71917892 | MS.gene70845:CDS | 30.0% | |
| AGAATCAGAAAGAGAAAGTA+CGG | + | chr5.3:71917737-71917756 | MS.gene70845:CDS | 30.0% | |
| ATTCAACAGAATCAACTACA+TGG | + | chr5.3:71918054-71918073 | MS.gene70845:CDS | 30.0% | |
| CTCAAATTAATAGAACACAG+TGG | + | chr5.3:71917102-71917121 | MS.gene70845:CDS | 30.0% | |
| CTTTCTGATTCTTCTTTCAT+TGG | - | chr5.3:71917729-71917748 | None:intergenic | 30.0% | |
| TGGAGTAAAGATGATATATC+AGG | + | chr5.3:71918074-71918093 | MS.gene70845:CDS | 30.0% | |
| ! | CAAATGCATTCACATCTTTT+CGG | - | chr5.3:71917248-71917267 | None:intergenic | 30.0% |
| ! | TCTATTAATTTGAGTCTGCT+AGG | - | chr5.3:71917096-71917115 | None:intergenic | 30.0% |
| ! | TTTGGTTTCTAATGTTGCTA+AGG | + | chr5.3:71917845-71917864 | MS.gene70845:CDS | 30.0% |
| !! | TGATGATTTTGATCCATTCA+AGG | + | chr5.3:71917479-71917498 | MS.gene70845:CDS | 30.0% |
| AAAGATGAGAACAAAAGAGG+TGG | + | chr5.3:71917876-71917895 | MS.gene70845:CDS | 35.0% | |
| AAGGTAAGAGAAGAGAAGAA+AGG | + | chr5.3:71917513-71917532 | MS.gene70845:CDS | 35.0% | |
| ACTCATGTAACATAACTCGT+TGG | - | chr5.3:71917019-71917038 | None:intergenic | 35.0% | |
| AGAGAAGAAAGGGAAATCAA+AGG | + | chr5.3:71917524-71917543 | MS.gene70845:CDS | 35.0% | |
| AGGTAAGAGAAGAGAAGAAA+GGG | + | chr5.3:71917514-71917533 | MS.gene70845:CDS | 35.0% | |
| ATGCAAACACCAATCTAGAT+CGG | + | chr5.3:71917166-71917185 | MS.gene70845:CDS | 35.0% | |
| CAAAGAAAAGACTTTGGCAA+TGG | - | chr5.3:71918134-71918153 | None:intergenic | 35.0% | |
| GAGAAGAAAGGGAAATCAAA+GGG | + | chr5.3:71917525-71917544 | MS.gene70845:CDS | 35.0% | |
| GCATCTTCATATGATGATCT+TGG | - | chr5.3:71917150-71917169 | None:intergenic | 35.0% | |
| GGCTAATGATACAAAGTTTG+AGG | + | chr5.3:71917812-71917831 | MS.gene70845:CDS | 35.0% | |
| ! | CCTTCTGGAAAAGACATTAA+AGG | - | chr5.3:71917678-71917697 | None:intergenic | 35.0% |
| ! | CCTTTAATGTCTTTTCCAGA+AGG | + | chr5.3:71917675-71917694 | MS.gene70845:CDS | 35.0% |
| ! | GTAGAGAGCATAGTTTAAGA+AGG | + | chr5.3:71917547-71917566 | MS.gene70845:CDS | 35.0% |
| ! | TAATGTCTTTTCCAGAAGGT+CGG | + | chr5.3:71917679-71917698 | MS.gene70845:CDS | 35.0% |
| ! | TTCTTTGGGAGTTCAAACAT+GGG | + | chr5.3:71917942-71917961 | MS.gene70845:CDS | 35.0% |
| ! | TTTCGGTTTGTCTTAGTTCT+CGG | - | chr5.3:71917231-71917250 | None:intergenic | 35.0% |
| ! | TTTCTTTGGGAGTTCAAACA+TGG | + | chr5.3:71917941-71917960 | MS.gene70845:CDS | 35.0% |
| !! | AAAGAGGTGGATTTTGGAAA+AGG | + | chr5.3:71917889-71917908 | MS.gene70845:CDS | 35.0% |
| !! | AGAACAAAAGAGGTGGATTT+TGG | + | chr5.3:71917883-71917902 | MS.gene70845:CDS | 35.0% |
| !! | AGAATTAGAGAAGCACTCTT+TGG | + | chr5.3:71918004-71918023 | MS.gene70845:CDS | 35.0% |
| !! | TATGATGATCTTGGTGTTGT+AGG | - | chr5.3:71917141-71917160 | None:intergenic | 35.0% |
| !! | TCTCCATTTTGAACTAGTCT+TGG | - | chr5.3:71917378-71917397 | None:intergenic | 35.0% |
| !! | TGATTTTGATCCATTCAAGG+TGG | + | chr5.3:71917482-71917501 | MS.gene70845:CDS | 35.0% |
| AACAAGTGTGACAAGCATGA+GGG | + | chr5.3:71917603-71917622 | MS.gene70845:CDS | 40.0% | |
| AGTCTTGGAGGAAGTTTAAG+AGG | - | chr5.3:71917363-71917382 | None:intergenic | 40.0% | |
| ATGCATTTGAGCAGCATCAA+AGG | + | chr5.3:71917259-71917278 | MS.gene70845:CDS | 40.0% | |
| GAACACAGTGGTTATCAAAC+AGG | + | chr5.3:71917114-71917133 | MS.gene70845:CDS | 40.0% | |
| GAATGCACAGATTTCATGTC+AGG | - | chr5.3:71918038-71918057 | None:intergenic | 40.0% | |
| TAGATCCCTTTCTCCATTGA+GGG | + | chr5.3:71917572-71917591 | MS.gene70845:CDS | 40.0% | |
| TCATCAGCAAATGCCATAGT+AGG | - | chr5.3:71917303-71917322 | None:intergenic | 40.0% | |
| TGAGGATTCATTGCCTACTA+TGG | + | chr5.3:71917287-71917306 | MS.gene70845:CDS | 40.0% | |
| TTCTCAAAAGCCACCTTGAA+TGG | - | chr5.3:71917495-71917514 | None:intergenic | 40.0% | |
| TTTCTTTGCCTGGGTTATGA+GGG | + | chr5.3:71918146-71918165 | MS.gene70845:CDS | 40.0% | |
| ! | CCAAAGTCTTTTCTTTGCCT+GGG | + | chr5.3:71918137-71918156 | MS.gene70845:CDS | 40.0% |
| ! | CCTCCAAGACTAGTTCAAAA+TGG | + | chr5.3:71917372-71917391 | MS.gene70845:CDS | 40.0% |
| ! | GTTTGAGGCTAAGAAGACTT+TGG | + | chr5.3:71917827-71917846 | MS.gene70845:CDS | 40.0% |
| ! | TTCTGACTTTTGTTGAGCAC+TGG | - | chr5.3:71917973-71917992 | None:intergenic | 40.0% |
| ! | TTGTTGTTGAAGTCCCTCAA+TGG | - | chr5.3:71917588-71917607 | None:intergenic | 40.0% |
| ! | TTTTCTTTGCCTGGGTTATG+AGG | + | chr5.3:71918145-71918164 | MS.gene70845:CDS | 40.0% |
| !! | ATTCAAGGTGGCTTTTGAGA+AGG | + | chr5.3:71917494-71917513 | MS.gene70845:CDS | 40.0% |
| !! | GCTGATGAGCTATTTTGTGA+TGG | + | chr5.3:71917315-71917334 | MS.gene70845:CDS | 40.0% |
| !!! | CCATTTTGAACTAGTCTTGG+AGG | - | chr5.3:71917375-71917394 | None:intergenic | 40.0% |
| AGTCTCCAGGATCAATGCTT+AGG | + | chr5.3:71917430-71917449 | MS.gene70845:CDS | 45.0% | |
| AGTTTAAGAGGCGGCATCAT+TGG | - | chr5.3:71917351-71917370 | None:intergenic | 45.0% | |
| CAACAAGTGTGACAAGCATG+AGG | + | chr5.3:71917602-71917621 | MS.gene70845:CDS | 45.0% | |
| CCCAGGCAAAGAAAAGACTT+TGG | - | chr5.3:71918140-71918159 | None:intergenic | 45.0% | |
| CTAGATCCCTTTCTCCATTG+AGG | + | chr5.3:71917571-71917590 | MS.gene70845:CDS | 45.0% | |
| CTTGGAGGAAGTTTAAGAGG+CGG | - | chr5.3:71917360-71917379 | None:intergenic | 45.0% | |
| GAAGTCCCTCAATGGAGAAA+GGG | - | chr5.3:71917580-71917599 | None:intergenic | 45.0% | |
| TCATGTAACAACCGACCTTC+TGG | - | chr5.3:71917693-71917712 | None:intergenic | 45.0% | |
| TCGGTTGTTACATGAGCTTC+TGG | + | chr5.3:71917698-71917717 | MS.gene70845:CDS | 45.0% | |
| TGAAGTCCCTCAATGGAGAA+AGG | - | chr5.3:71917581-71917600 | None:intergenic | 45.0% | |
| TTCTTCCACAAGCGTGCAAA+AGG | - | chr5.3:71917459-71917478 | None:intergenic | 45.0% | |
| ! | GCCAAAGTCTTTTCTTTGCC+TGG | + | chr5.3:71918136-71918155 | MS.gene70845:CDS | 45.0% |
| ! | TTGAGTCTGCTAGGACTAGT+TGG | - | chr5.3:71917087-71917106 | None:intergenic | 45.0% |
| !! | AGCATTGATCCTGGAGACTT+AGG | - | chr5.3:71917429-71917448 | None:intergenic | 45.0% |
| ACAGGTGAGACAAGTGGAAG+TGG | + | chr5.3:71917791-71917810 | MS.gene70845:CDS | 50.0% | |
| AGTACGGTATCTGAGCCCAA+AGG | + | chr5.3:71917753-71917772 | MS.gene70845:CDS | 50.0% | |
| CTTGCAAACGCCAACCCTTT+GGG | - | chr5.3:71917771-71917790 | None:intergenic | 50.0% | |
| CTTTCCATCCCTCATAACCC+AGG | - | chr5.3:71918157-71918176 | None:intergenic | 50.0% | |
| GTACGGTATCTGAGCCCAAA+GGG | + | chr5.3:71917754-71917773 | MS.gene70845:CDS | 50.0% | |
| TCTTGCAAACGCCAACCCTT+TGG | - | chr5.3:71917772-71917791 | None:intergenic | 50.0% | |
| TGCAAGACAGGTGAGACAAG+TGG | + | chr5.3:71917785-71917804 | MS.gene70845:CDS | 50.0% | |
| TTTGCCTGGGTTATGAGGGA+TGG | + | chr5.3:71918150-71918169 | MS.gene70845:CDS | 50.0% | |
| ! | GCAGCATCAAAGGCTTTGTG+AGG | + | chr5.3:71917269-71917288 | MS.gene70845:CDS | 50.0% |
| !! | GGAAGCCTAAGCATTGATCC+TGG | - | chr5.3:71917438-71917457 | None:intergenic | 50.0% |
| GGTATCTGAGCCCAAAGGGT+TGG | + | chr5.3:71917758-71917777 | MS.gene70845:CDS | 55.0% | |
| ! | AGGGTTGGCGTTTGCAAGAC+AGG | + | chr5.3:71917773-71917792 | MS.gene70845:CDS | 55.0% |
| ! | GGCTTCCTTTTGCACGCTTG+TGG | + | chr5.3:71917451-71917470 | MS.gene70845:CDS | 55.0% |
| GCGACGTCTCCTAAGTCTCC+AGG | + | chr5.3:71917417-71917436 | MS.gene70845:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 71916997 | 71918184 | 71916997 | ID=MS.gene70845 |
| chr5.3 | mRNA | 71916997 | 71918184 | 71916997 | ID=MS.gene70845.t1;Parent=MS.gene70845 |
| chr5.3 | exon | 71916997 | 71918184 | 71916997 | ID=MS.gene70845.t1.exon1;Parent=MS.gene70845.t1 |
| chr5.3 | CDS | 71916997 | 71918184 | 71916997 | ID=cds.MS.gene70845.t1;Parent=MS.gene70845.t1 |
| Gene Sequence |
| Protein sequence |
