Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71213.t1 | XP_003628762.1 | 97.8 | 277 | 6 | 0 | 9 | 285 | 3 | 279 | 1.50E-122 | 449.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71213.t1 | Q9LFD5 | 53.4 | 247 | 107 | 3 | 9 | 255 | 5 | 243 | 9.1e-52 | 205.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71213.t1 | G7LGY9 | 97.8 | 277 | 6 | 0 | 9 | 285 | 3 | 279 | 1.1e-122 | 449.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene41753 | MS.gene71213 | PPI |
| MS.gene067833 | MS.gene71213 | PPI |
| MS.gene08184 | MS.gene71213 | PPI |
| MS.gene042447 | MS.gene71213 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71213.t1 | MTR_8g066460 | 97.834 | 277 | 6 | 0 | 9 | 285 | 3 | 279 | 0.0 | 541 |
| MS.gene71213.t1 | MTR_8g066460 | 97.834 | 277 | 6 | 0 | 9 | 285 | 19 | 295 | 0.0 | 540 |
| MS.gene71213.t1 | MTR_5g029640 | 80.952 | 252 | 48 | 0 | 9 | 260 | 3 | 254 | 5.55e-144 | 413 |
| MS.gene71213.t1 | MTR_5g029640 | 78.205 | 156 | 34 | 0 | 113 | 268 | 279 | 434 | 5.90e-74 | 234 |
| MS.gene71213.t1 | MTR_1g032860 | 56.202 | 258 | 101 | 3 | 9 | 259 | 31 | 283 | 4.98e-95 | 283 |
| MS.gene71213.t1 | MTR_8g105910 | 52.963 | 270 | 117 | 2 | 9 | 278 | 31 | 290 | 2.68e-93 | 278 |
| MS.gene71213.t1 | MTR_7g086080 | 50.202 | 247 | 105 | 3 | 10 | 240 | 4 | 248 | 6.81e-74 | 227 |
| MS.gene71213.t1 | MTR_5g039130 | 38.750 | 240 | 128 | 4 | 12 | 236 | 10 | 245 | 1.31e-46 | 157 |
| MS.gene71213.t1 | MTR_7g010100 | 32.157 | 255 | 135 | 5 | 11 | 236 | 7 | 252 | 3.03e-31 | 117 |
| MS.gene71213.t1 | MTR_5g038107 | 68.333 | 60 | 19 | 0 | 9 | 68 | 3 | 62 | 2.03e-22 | 90.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71213.t1 | AT4G17720 | 68.939 | 264 | 79 | 2 | 11 | 271 | 5 | 268 | 1.83e-122 | 352 |
| MS.gene71213.t1 | AT5G46870 | 62.319 | 276 | 95 | 3 | 11 | 277 | 5 | 280 | 3.02e-110 | 321 |
| MS.gene71213.t1 | AT5G46870 | 62.319 | 276 | 95 | 3 | 11 | 277 | 5 | 280 | 3.02e-110 | 321 |
| MS.gene71213.t1 | AT5G16840 | 64.957 | 234 | 75 | 4 | 9 | 242 | 5 | 231 | 1.06e-101 | 298 |
| MS.gene71213.t1 | AT5G16840 | 64.957 | 234 | 74 | 5 | 9 | 242 | 5 | 230 | 4.19e-100 | 294 |
| MS.gene71213.t1 | AT5G16840 | 64.957 | 234 | 74 | 5 | 9 | 242 | 3 | 228 | 4.93e-100 | 293 |
| MS.gene71213.t1 | AT1G67950 | 52.766 | 235 | 101 | 2 | 9 | 243 | 28 | 252 | 5.92e-80 | 243 |
| MS.gene71213.t1 | AT1G67950 | 52.766 | 235 | 100 | 3 | 9 | 243 | 28 | 251 | 2.12e-78 | 239 |
| MS.gene71213.t1 | AT1G67950 | 52.766 | 235 | 100 | 3 | 9 | 243 | 29 | 252 | 3.23e-78 | 239 |
| MS.gene71213.t1 | AT5G32450 | 49.600 | 250 | 111 | 2 | 6 | 240 | 1 | 250 | 5.02e-75 | 230 |
| MS.gene71213.t1 | AT1G67950 | 49.754 | 203 | 91 | 3 | 41 | 243 | 1 | 192 | 8.04e-60 | 190 |
| MS.gene71213.t1 | AT1G14340 | 36.441 | 236 | 135 | 3 | 12 | 236 | 9 | 240 | 1.21e-40 | 141 |
| MS.gene71213.t1 | AT3G01210 | 32.520 | 246 | 132 | 5 | 13 | 234 | 9 | 244 | 9.83e-30 | 113 |
| MS.gene71213.t1 | AT3G01210 | 32.520 | 246 | 132 | 5 | 13 | 234 | 9 | 244 | 9.83e-30 | 113 |
Find 69 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCTAAGGGATTTATCTT+AGG | 0.185356 | 8.3:-36499001 | MS.gene71213:CDS |
| TCACTTGTAGCTGCGGCTTT+TGG | 0.194364 | 8.3:+36498569 | None:intergenic |
| AGAAGTGGATCAAAAGTTTC+AGG | 0.259324 | 8.3:-36498831 | MS.gene71213:CDS |
| GAAGATGAACGGGGCTGTTT+TGG | 0.265277 | 8.3:-36506956 | None:intergenic |
| CAAACTTCCACCTGCTGCTT+TGG | 0.312762 | 8.3:-36499382 | MS.gene71213:CDS |
| AATTGCCTTTGTTACTTTCA+AGG | 0.314569 | 8.3:-36502202 | MS.gene71213:CDS |
| AGCAGCTGCAAATGCTGATT+TGG | 0.315915 | 8.3:+36498796 | None:intergenic |
| ACTGGTACTACCTGGGTAAC+TGG | 0.368921 | 8.3:-36498719 | MS.gene71213:CDS |
| TGCCTAAATAGAAGATGAAC+GGG | 0.400078 | 8.3:-36506966 | None:intergenic |
| TTGCTAAGGGATTTATCTTA+GGG | 0.407617 | 8.3:-36499000 | MS.gene71213:CDS |
| AAAAGTCGGTAATGTTTCCT+TGG | 0.410188 | 8.3:-36502423 | MS.gene71213:CDS |
| AGTAAGGTTGCTAAGGCAGC+TGG | 0.411693 | 8.3:-36498689 | MS.gene71213:CDS |
| AAAATGAAAAGAACTCCTTT+AGG | 0.412585 | 8.3:+36502384 | None:intergenic |
| TTGAATATGTTGAAATGAAA+AGG | 0.433319 | 8.3:-36502352 | MS.gene71213:intron |
| TTCCAAGCCTACACCTGCTC+AGG | 0.433849 | 8.3:-36498537 | MS.gene71213:CDS |
| CCGAGGCTGTTGAACTCAGC+TGG | 0.437770 | 8.3:+36498936 | None:intergenic |
| GCAGGTGGAAGTTTGTAATC+TGG | 0.442443 | 8.3:+36499390 | None:intergenic |
| CCAGTATGCGCAGGTCCTTT+CGG | 0.448214 | 8.3:-36498597 | MS.gene71213:CDS |
| GTGGTGCCGACTCTGCTTTA+CGG | 0.453078 | 8.3:-36499051 | MS.gene71213:CDS |
| AAAGTCGGTAATGTTTCCTT+GGG | 0.468203 | 8.3:-36502422 | MS.gene71213:CDS |
| GCTAAGGCAGCTGGGGATGT+AGG | 0.476446 | 8.3:-36498680 | MS.gene71213:CDS |
| CCGAAAGGACCTGCGCATAC+TGG | 0.482179 | 8.3:+36498597 | None:intergenic |
| TTTGTCAGTTAACATAACTC+TGG | 0.492790 | 8.3:-36499415 | MS.gene71213:CDS |
| CGATAGTAGTACTGCCGTCT+CGG | 0.498280 | 8.3:+36502167 | None:intergenic |
| ACCCTGAGCAGGTGTAGGCT+TGG | 0.499327 | 8.3:+36498535 | None:intergenic |
| ACAGATGATGCCAAAGCAGC+AGG | 0.499671 | 8.3:+36499372 | None:intergenic |
| ACAGAAAACAAAAGAGAAAG+TGG | 0.508009 | 8.3:-36498657 | MS.gene71213:CDS |
| GCCGCTGAGGGTAAAACTCC+TGG | 0.513612 | 8.3:-36499073 | MS.gene71213:CDS |
| CAAAGGATCAAACCCTGAGC+AGG | 0.517360 | 8.3:+36498524 | None:intergenic |
| TGTCACCAGCATGCTTGCTA+AGG | 0.522299 | 8.3:-36499014 | MS.gene71213:CDS |
| GTCACCAGCATGCTTGCTAA+GGG | 0.532621 | 8.3:-36499013 | MS.gene71213:CDS |
| ATATACTCACTGGTACTACC+TGG | 0.533676 | 8.3:-36498727 | MS.gene71213:CDS |
| CCAGCTGAGTTCAACAGCCT+CGG | 0.534083 | 8.3:-36498936 | MS.gene71213:CDS |
| GAACAGAAAGTCAGTACCGC+AGG | 0.537626 | 8.3:-36498773 | MS.gene71213:CDS |
| AGCATCTGAACAAGACCTAA+AGG | 0.544372 | 8.3:-36502399 | MS.gene71213:CDS |
| GAAAGTGGAAAATGCAGAAG+TGG | 0.544685 | 8.3:-36498642 | MS.gene71213:CDS |
| GTAAGGTTGCTAAGGCAGCT+GGG | 0.546936 | 8.3:-36498688 | MS.gene71213:CDS |
| GTCTTGTTCAGATGCTCCCA+AGG | 0.552262 | 8.3:+36502406 | None:intergenic |
| TCCAAGCCTACACCTGCTCA+GGG | 0.564645 | 8.3:-36498536 | MS.gene71213:CDS |
| AAAGCAGAGTCGGCACCACC+AGG | 0.566278 | 8.3:+36499055 | None:intergenic |
| CAGGTCAAAACTATAAAAGT+CGG | 0.570204 | 8.3:-36502437 | MS.gene71213:CDS |
| GATGCTGTGAACAAAGCAAA+GGG | 0.570764 | 8.3:-36498974 | MS.gene71213:CDS |
| GAGTGGTAGAGTTAAAGAAG+TGG | 0.581041 | 8.3:-36498846 | MS.gene71213:CDS |
| AAGAATCGCTATATACTCAC+TGG | 0.582491 | 8.3:-36498737 | MS.gene71213:CDS |
| CTCTGCTTTACGGAAGGCAG+AGG | 0.586688 | 8.3:-36499041 | MS.gene71213:CDS |
| GTGCCTAAATAGAAGATGAA+CGG | 0.587388 | 8.3:-36506967 | None:intergenic |
| GGATCCACAAGGTGCCGAGA+CGG | 0.588318 | 8.3:-36502181 | MS.gene71213:CDS |
| AGATGCTGTGAACAAAGCAA+AGG | 0.591752 | 8.3:-36498975 | MS.gene71213:CDS |
| ACTGCCGTCTCGGCACCTTG+TGG | 0.593659 | 8.3:+36502177 | None:intergenic |
| TGCCGACTCTGCTTTACGGA+AGG | 0.594224 | 8.3:-36499047 | MS.gene71213:CDS |
| AAATCCCTTAGCAAGCATGC+TGG | 0.596828 | 8.3:+36499009 | None:intergenic |
| GTGCAGTTCACTTGTAGCTG+CGG | 0.599368 | 8.3:+36498562 | None:intergenic |
| AGTTGAAGACCAGTATGCGC+AGG | 0.604810 | 8.3:-36498606 | MS.gene71213:CDS |
| TGCCTTCCGTAAAGCAGAGT+CGG | 0.606291 | 8.3:+36499045 | None:intergenic |
| TGGATCCTTGAAAGTAACAA+AGG | 0.609650 | 8.3:+36502197 | None:intergenic |
| CTTAGTGAGAAATTAACTGC+TGG | 0.614592 | 8.3:-36498881 | MS.gene71213:CDS |
| ACTAAAAGCACCAGTTACCC+AGG | 0.621013 | 8.3:+36498709 | None:intergenic |
| TATACTCACTGGTACTACCT+GGG | 0.629105 | 8.3:-36498726 | MS.gene71213:CDS |
| GATGATGCCAAAGCAGCAGG+TGG | 0.631195 | 8.3:+36499375 | None:intergenic |
| TAAGGTTGCTAAGGCAGCTG+GGG | 0.631391 | 8.3:-36498687 | MS.gene71213:CDS |
| CGAGACGGCAGTACTACTAT+CGG | 0.643990 | 8.3:-36502166 | MS.gene71213:intron |
| ATCAAACCCTGAGCAGGTGT+AGG | 0.644936 | 8.3:+36498530 | None:intergenic |
| GTTACTTTCAAGGATCCACA+AGG | 0.648181 | 8.3:-36502192 | MS.gene71213:CDS |
| AACAGAAAGTCAGTACCGCA+GGG | 0.666312 | 8.3:-36498772 | MS.gene71213:CDS |
| GCTGAGGGTAAAACTCCTGG+TGG | 0.693763 | 8.3:-36499070 | MS.gene71213:CDS |
| AAAAGATGTAACTTTCGCCG+AGG | 0.709369 | 8.3:+36498919 | None:intergenic |
| GCTGGTGCATCAGTTGTGAG+TGG | 0.711756 | 8.3:-36498863 | MS.gene71213:CDS |
| CTTCATTATAGCAGACCCTG+CGG | 0.733885 | 8.3:+36498757 | None:intergenic |
| GCCTAAATAGAAGATGAACG+GGG | 0.780758 | 8.3:-36506965 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 36498523 | 36506974 | 36498523 | ID=MS.gene71213 |
| chr8.3 | mRNA | 36498523 | 36506974 | 36498523 | ID=MS.gene71213.t1;Parent=MS.gene71213 |
| chr8.3 | exon | 36506951 | 36506974 | 36506951 | ID=MS.gene71213.t1.exon1;Parent=MS.gene71213.t1 |
| chr8.3 | CDS | 36506951 | 36506974 | 36506951 | ID=cds.MS.gene71213.t1;Parent=MS.gene71213.t1 |
| chr8.3 | exon | 36502353 | 36502456 | 36502353 | ID=MS.gene71213.t1.exon2;Parent=MS.gene71213.t1 |
| chr8.3 | CDS | 36502353 | 36502456 | 36502353 | ID=cds.MS.gene71213.t1;Parent=MS.gene71213.t1 |
| chr8.3 | exon | 36502167 | 36502242 | 36502167 | ID=MS.gene71213.t1.exon3;Parent=MS.gene71213.t1 |
| chr8.3 | CDS | 36502167 | 36502242 | 36502167 | ID=cds.MS.gene71213.t1;Parent=MS.gene71213.t1 |
| chr8.3 | exon | 36499374 | 36499454 | 36499374 | ID=MS.gene71213.t1.exon4;Parent=MS.gene71213.t1 |
| chr8.3 | CDS | 36499374 | 36499454 | 36499374 | ID=cds.MS.gene71213.t1;Parent=MS.gene71213.t1 |
| chr8.3 | exon | 36498523 | 36499095 | 36498523 | ID=MS.gene71213.t1.exon5;Parent=MS.gene71213.t1 |
| chr8.3 | CDS | 36498523 | 36499095 | 36498523 | ID=cds.MS.gene71213.t1;Parent=MS.gene71213.t1 |
| Gene Sequence |
| Protein sequence |
