Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71430.t1 | XP_028762943.1 | 63.8 | 94 | 28 | 1 | 12 | 99 | 255 | 348 | 2.00E-26 | 128.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71430.t1 | A0A392P9H0 | 64.4 | 90 | 31 | 1 | 2 | 91 | 107 | 195 | 4.7e-25 | 123.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049531 | MS.gene71430 | 0.81247 | 4.17E-51 | -1.69E-46 |
| MS.gene050221 | MS.gene71430 | 0.804862 | 1.76E-49 | -1.69E-46 |
| MS.gene051710 | MS.gene71430 | 0.809147 | 2.19E-50 | -1.69E-46 |
| MS.gene058515 | MS.gene71430 | 0.808278 | 3.35E-50 | -1.69E-46 |
| MS.gene059272 | MS.gene71430 | 0.823446 | 1.38E-53 | -1.69E-46 |
| MS.gene059276 | MS.gene71430 | 0.808716 | 2.70E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71430.t1 | MTR_1g036690 | 58.537 | 82 | 27 | 3 | 19 | 99 | 5 | 80 | 2.63e-25 | 90.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTTCAAAATAAGGATAAA+AGG | 0.234592 | 1.3:-54102147 | MS.gene71430:CDS |
| CCAACAATCACTTCAAAATA+AGG | 0.244294 | 1.3:-54102155 | MS.gene71430:CDS |
| TATAGGTGGGGTTCTATTCC+TGG | 0.331534 | 1.3:+54101993 | None:intergenic |
| TAAAGATCAACAAGATGTTT+TGG | 0.333763 | 1.3:+54102178 | None:intergenic |
| TGAAGATGATTATGGTAATA+TGG | 0.338642 | 1.3:-54102095 | MS.gene71430:CDS |
| GAAAATGATGAAGATGATTA+TGG | 0.391347 | 1.3:-54102103 | MS.gene71430:CDS |
| TGCTATCGTTGTGGAGGAAA+AGG | 0.414829 | 1.3:-54102226 | MS.gene71430:CDS |
| AGAAATCAGCAGCATCTAAG+TGG | 0.464577 | 1.3:+54102065 | None:intergenic |
| AATGTTGTAAAGAATGATAT+TGG | 0.471241 | 1.3:-54102277 | MS.gene71430:CDS |
| ACTTCAAAATAAGGATAAAA+GGG | 0.487291 | 1.3:-54102146 | MS.gene71430:CDS |
| GTTGTGGAGGAAAAGGTCAT+TGG | 0.504176 | 1.3:-54102219 | MS.gene71430:CDS |
| CATTAGAGATGAAAATATCC+AGG | 0.532865 | 1.3:-54102011 | MS.gene71430:CDS |
| GAGAGTATTTGCTATCGTTG+TGG | 0.583351 | 1.3:-54102235 | MS.gene71430:CDS |
| AGTATTTGCTATCGTTGTGG+AGG | 0.599695 | 1.3:-54102232 | MS.gene71430:CDS |
| GAAATCAGCAGCATCTAAGT+GGG | 0.621669 | 1.3:+54102066 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGTAAAGAATGATAT+TGG | - | chr1.3:54102012-54102031 | MS.gene71430:CDS | 20.0% |
| !! | ACTTCAAAATAAGGATAAAA+GGG | - | chr1.3:54102143-54102162 | MS.gene71430:CDS | 20.0% |
| ! | CACTTCAAAATAAGGATAAA+AGG | - | chr1.3:54102142-54102161 | MS.gene71430:CDS | 25.0% |
| ! | GAAAATGATGAAGATGATTA+TGG | - | chr1.3:54102186-54102205 | MS.gene71430:CDS | 25.0% |
| ! | TGAAGATGATTATGGTAATA+TGG | - | chr1.3:54102194-54102213 | MS.gene71430:CDS | 25.0% |
| !! | TAAAGATCAACAAGATGTTT+TGG | + | chr1.3:54102114-54102133 | None:intergenic | 25.0% |
| !! | TATTTTCATCTCTAATGAGA+TGG | + | chr1.3:54102275-54102294 | None:intergenic | 25.0% |
| CATTAGAGATGAAAATATCC+AGG | - | chr1.3:54102278-54102297 | MS.gene71430:CDS | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | - | chr1.3:54102134-54102153 | MS.gene71430:CDS | 30.0% | |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | + | chr1.3:54102137-54102156 | None:intergenic | 30.0% |
| ! | AGATGGTCAATCTTTTCATC+AGG | + | chr1.3:54102258-54102277 | None:intergenic | 35.0% |
| AGAAATCAGCAGCATCTAAG+TGG | + | chr1.3:54102227-54102246 | None:intergenic | 40.0% | |
| GAAATCAGCAGCATCTAAGT+GGG | + | chr1.3:54102226-54102245 | None:intergenic | 40.0% | |
| !! | AGTATTTGCTATCGTTGTGG+AGG | - | chr1.3:54102057-54102076 | MS.gene71430:CDS | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | - | chr1.3:54102054-54102073 | MS.gene71430:CDS | 40.0% |
| GTTGTGGAGGAAAAGGTCAT+TGG | - | chr1.3:54102070-54102089 | MS.gene71430:CDS | 45.0% | |
| TGCTATCGTTGTGGAGGAAA+AGG | - | chr1.3:54102063-54102082 | MS.gene71430:CDS | 45.0% | |
| !!! | AAGATGTTTTGGCGTACGAC+AGG | + | chr1.3:54102103-54102122 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 54102006 | 54102305 | 54102006 | ID=MS.gene71430 |
| chr1.3 | mRNA | 54102006 | 54102305 | 54102006 | ID=MS.gene71430.t1;Parent=MS.gene71430 |
| chr1.3 | exon | 54102006 | 54102305 | 54102006 | ID=MS.gene71430.t1.exon1;Parent=MS.gene71430.t1 |
| chr1.3 | CDS | 54102006 | 54102305 | 54102006 | ID=cds.MS.gene71430.t1;Parent=MS.gene71430.t1 |
| Gene Sequence |
| Protein sequence |
