Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71587.t1 | XP_013469954.1 | 96.3 | 136 | 5 | 0 | 1 | 136 | 79 | 214 | 5.20E-65 | 256.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71587.t1 | A0A072VQC5 | 96.3 | 136 | 5 | 0 | 1 | 136 | 79 | 214 | 3.8e-65 | 256.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049757 | MS.gene71587 | 0.80219 | 6.31E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene71587 | 0.826851 | 2.16E-54 | -1.69E-46 |
| MS.gene053912 | MS.gene71587 | 0.833958 | 3.95E-56 | -1.69E-46 |
| MS.gene056387 | MS.gene71587 | 0.819938 | 8.93E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71587.t1 | MTR_1g105970 | 96.324 | 136 | 5 | 0 | 1 | 136 | 79 | 214 | 4.07e-94 | 270 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71587.t1 | AT1G50020 | 59.124 | 137 | 50 | 2 | 1 | 136 | 78 | 209 | 6.17e-52 | 164 |
Find 38 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCTCCTCGGCTAAATTAA+AGG | 0.278659 | 1.4:+81799264 | MS.gene71587:CDS |
| GGAACATTCTTCTTGGCATT+TGG | 0.330859 | 1.4:+81798360 | MS.gene71587:CDS |
| GAATCAGAGGGAACGGTCTA+TGG | 0.337234 | 1.4:+81797684 | MS.gene71587:CDS |
| ACTTGGAGGAACATTCTTCT+TGG | 0.347709 | 1.4:+81798353 | MS.gene71587:CDS |
| CTTCTGTGAATGAAGAAGAT+AGG | 0.362753 | 1.4:+81797611 | MS.gene71587:CDS |
| GATTACAAGCAAACCAACTA+TGG | 0.380799 | 1.4:+81797574 | MS.gene71587:CDS |
| CTTCCGTCGTGGACAAAACT+TGG | 0.396329 | 1.4:-81799170 | None:intergenic |
| TCATCTTCTAAGAGTGCATA+AGG | 0.401040 | 1.4:-81799227 | None:intergenic |
| CTCTTCATCAACTCCATAGT+TGG | 0.402993 | 1.4:-81797587 | None:intergenic |
| TTCTGTGAATGAAGAAGATA+GGG | 0.460643 | 1.4:+81797612 | MS.gene71587:CDS |
| AAACTTCTATTGACACTTGG+AGG | 0.515017 | 1.4:+81798339 | MS.gene71587:CDS |
| TAATCCTCAAGCAATGATGT+AGG | 0.515998 | 1.4:-81797556 | None:intergenic |
| AATCCTCAAGCAATGATGTA+GGG | 0.519413 | 1.4:-81797555 | None:intergenic |
| ACTATCACCTCCACAATATG+TGG | 0.522313 | 1.4:+81799202 | MS.gene71587:CDS |
| CTCTAGACCCCTCTCAACAT+TGG | 0.523903 | 1.4:-81797662 | None:intergenic |
| CATCTTCTAAGAGTGCATAA+GGG | 0.537509 | 1.4:-81799226 | None:intergenic |
| GCATAAGGGTCCACATATTG+TGG | 0.543635 | 1.4:-81799212 | None:intergenic |
| AGAGGGGTCTAGAGAATCAG+AGG | 0.544137 | 1.4:+81797671 | MS.gene71587:CDS |
| GTCTAGAGAATCAGAGGGAA+CGG | 0.571889 | 1.4:+81797677 | MS.gene71587:CDS |
| GACCCCTACATCATTGCTTG+AGG | 0.575241 | 1.4:+81797552 | MS.gene71587:CDS |
| ATGCACTCTTAGAAGATGAA+AGG | 0.586584 | 1.4:+81799231 | MS.gene71587:CDS |
| GCTAAACTTCTATTGACACT+TGG | 0.589619 | 1.4:+81798336 | MS.gene71587:CDS |
| AGTGTCAACCAATGTTGAGA+GGG | 0.601124 | 1.4:+81797654 | MS.gene71587:CDS |
| GCCACGGTTATGAGGATCAA+CGG | 0.602581 | 1.4:-81798384 | None:intergenic |
| CAACTATGGAGTTGATGAAG+AGG | 0.608576 | 1.4:+81797588 | MS.gene71587:CDS |
| CAGTGTCAACCAATGTTGAG+AGG | 0.610884 | 1.4:+81797653 | MS.gene71587:CDS |
| GGATATCTCAGATAGCTCCT+CGG | 0.629394 | 1.4:+81799252 | MS.gene71587:CDS |
| GTGACATCTTGCTTCCGTCG+TGG | 0.633182 | 1.4:-81799181 | None:intergenic |
| GCTGAACAGTGAAGGTCTTG+AGG | 0.634680 | 1.4:+81797708 | MS.gene71587:CDS |
| ATCCTCAAGCAATGATGTAG+GGG | 0.635911 | 1.4:-81797554 | None:intergenic |
| TCTATGGCGCTGAACAGTGA+AGG | 0.641145 | 1.4:+81797700 | MS.gene71587:CDS |
| GTCAATAGAAGTTTAGCTCG+AGG | 0.648431 | 1.4:-81798330 | None:intergenic |
| GAGGGGTCTAGAGAATCAGA+GGG | 0.670863 | 1.4:+81797672 | MS.gene71587:CDS |
| TAAGGGTCCACATATTGTGG+AGG | 0.678073 | 1.4:-81799209 | None:intergenic |
| AGAGCTTACGAAGTAAAGAG+CGG | 0.697103 | 1.4:-81798415 | None:intergenic |
| GTGTCAACCAATGTTGAGAG+GGG | 0.698061 | 1.4:+81797655 | MS.gene71587:CDS |
| ACCGTTGATCCTCATAACCG+TGG | 0.700978 | 1.4:+81798383 | MS.gene71587:CDS |
| TCTGTGAATGAAGAAGATAG+GGG | 0.715285 | 1.4:+81797613 | MS.gene71587:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTTACTACAATACAAATA+AGG | - | chr1.4:81798193-81798212 | None:intergenic | 15.0% |
| !!! | AGAATATACATTTTTTCTAA+AGG | - | chr1.4:81798664-81798683 | None:intergenic | 15.0% |
| !!! | TTTTACATACTTTCTAAATA+GGG | + | chr1.4:81798163-81798182 | MS.gene71587:intron | 15.0% |
| !! | ATAAATATGTAAGTGATTTC+TGG | - | chr1.4:81798292-81798311 | None:intergenic | 20.0% |
| !! | ATAGTACACATTTAATGATA+TGG | + | chr1.4:81798505-81798524 | MS.gene71587:intron | 20.0% |
| !! | TAATGTTAAACATAAACCAT+AGG | - | chr1.4:81798897-81798916 | None:intergenic | 20.0% |
| !! | TTACATATTTATCCATTTCA+GGG | + | chr1.4:81798300-81798319 | MS.gene71587:intron | 20.0% |
| !!! | GTTTTACATACTTTCTAAAT+AGG | + | chr1.4:81798162-81798181 | MS.gene71587:intron | 20.0% |
| !!! | TATATAAAAAATCGCTGATT+TGG | + | chr1.4:81797762-81797781 | MS.gene71587:intron | 20.0% |
| ! | CTTACATATTTATCCATTTC+AGG | + | chr1.4:81798299-81798318 | MS.gene71587:intron | 25.0% |
| ! | TATTGAAGGAAAGTTAACAA+TGG | - | chr1.4:81797739-81797758 | None:intergenic | 25.0% |
| ! | TGAAGTGAATAACAATTTGA+TGG | + | chr1.4:81798567-81798586 | MS.gene71587:intron | 25.0% |
| !!! | ATATTCTAGCTTATAACAGT+TGG | + | chr1.4:81798677-81798696 | MS.gene71587:intron | 25.0% |
| AACATAAACCATAGGATAGA+TGG | - | chr1.4:81798889-81798908 | None:intergenic | 30.0% | |
| ACATAAACCATAGGATAGAT+GGG | - | chr1.4:81798888-81798907 | None:intergenic | 30.0% | |
| ACTAAGACTTCAGAACATTT+AGG | + | chr1.4:81798621-81798640 | MS.gene71587:intron | 30.0% | |
| ATAAACAGCCAAATCAAGTT+TGG | - | chr1.4:81797902-81797921 | None:intergenic | 30.0% | |
| CAACGTTCTATATTACATGA+AGG | - | chr1.4:81799115-81799134 | None:intergenic | 30.0% | |
| CATGTAATATAGAACGTTGA+TGG | + | chr1.4:81799116-81799135 | MS.gene71587:intron | 30.0% | |
| TAACATCTTGATCTGATGTT+TGG | + | chr1.4:81797938-81797957 | MS.gene71587:intron | 30.0% | |
| TAGATTACTTAACACGCAAA+CGG | - | chr1.4:81798484-81798503 | None:intergenic | 30.0% | |
| TTCTGTGAATGAAGAAGATA+GGG | + | chr1.4:81797612-81797631 | MS.gene71587:CDS | 30.0% | |
| TTTGTGCTTGTATGAAATAG+TGG | + | chr1.4:81799077-81799096 | MS.gene71587:intron | 30.0% | |
| ! | AATAGTCTTCAGTTTTCAAC+AGG | - | chr1.4:81798960-81798979 | None:intergenic | 30.0% |
| ! | ATAGTCTTCAGTTTTCAACA+GGG | - | chr1.4:81798959-81798978 | None:intergenic | 30.0% |
| ! | ATCTGATGTTTGGAACTTAA+AGG | + | chr1.4:81797948-81797967 | MS.gene71587:intron | 30.0% |
| ! | GTTTTTGTGCATTCAATTCT+TGG | + | chr1.4:81798810-81798829 | MS.gene71587:intron | 30.0% |
| ! | TGTAAGCTGTATTTCTATGA+TGG | - | chr1.4:81798251-81798270 | None:intergenic | 30.0% |
| ! | TTGATTTTGTGTCTGTTGTT+TGG | + | chr1.4:81798751-81798770 | MS.gene71587:intron | 30.0% |
| !! | TCTTAGTTTTCCAATTGAGT+TGG | - | chr1.4:81798608-81798627 | None:intergenic | 30.0% |
| !!! | ATTTTTTCTAAAGGAAGCTG+AGG | - | chr1.4:81798655-81798674 | None:intergenic | 30.0% |
| !!! | CTGTTTTTGTTGCAGTATTT+CGG | + | chr1.4:81799146-81799165 | MS.gene71587:intron | 30.0% |
| AAAAAAATGCCACGGTTATG+AGG | - | chr1.4:81798395-81798414 | None:intergenic | 35.0% | |
| AAACTTCTATTGACACTTGG+AGG | + | chr1.4:81798339-81798358 | MS.gene71587:CDS | 35.0% | |
| AATCCTCAAGCAATGATGTA+GGG | - | chr1.4:81797558-81797577 | None:intergenic | 35.0% | |
| ACAGAACACATTACATTAGC+TGG | - | chr1.4:81798449-81798468 | None:intergenic | 35.0% | |
| ATGAGAATAGAGTTCTTCAG+CGG | - | chr1.4:81798054-81798073 | None:intergenic | 35.0% | |
| ATGCACTCTTAGAAGATGAA+AGG | + | chr1.4:81799231-81799250 | MS.gene71587:CDS | 35.0% | |
| CTCAATGTGAAGAATGAATG+AGG | - | chr1.4:81799053-81799072 | None:intergenic | 35.0% | |
| CTTCTGTGAATGAAGAAGAT+AGG | + | chr1.4:81797611-81797630 | MS.gene71587:CDS | 35.0% | |
| GATTACAAGCAAACCAACTA+TGG | + | chr1.4:81797574-81797593 | MS.gene71587:CDS | 35.0% | |
| GCTAAACTTCTATTGACACT+TGG | + | chr1.4:81798336-81798355 | MS.gene71587:CDS | 35.0% | |
| TAATCCTCAAGCAATGATGT+AGG | - | chr1.4:81797559-81797578 | None:intergenic | 35.0% | |
| TATTTATCCATTTCAGGGCT+TGG | + | chr1.4:81798305-81798324 | MS.gene71587:intron | 35.0% | |
| TCAAACCACCAAACTTGATT+TGG | + | chr1.4:81797891-81797910 | MS.gene71587:intron | 35.0% | |
| TCTGTGAATGAAGAAGATAG+GGG | + | chr1.4:81797613-81797632 | MS.gene71587:CDS | 35.0% | |
| TGAAGAATGAATGAGGATTG+AGG | - | chr1.4:81799046-81799065 | None:intergenic | 35.0% | |
| ! | ATTTTCTGTTTGCGTCATGT+TGG | + | chr1.4:81798995-81799014 | MS.gene71587:intron | 35.0% |
| ! | CATCTTCTAAGAGTGCATAA+GGG | - | chr1.4:81799229-81799248 | None:intergenic | 35.0% |
| ! | TCATCTTCTAAGAGTGCATA+AGG | - | chr1.4:81799230-81799249 | None:intergenic | 35.0% |
| ! | TTGGAACTTAAAGGTTCTGA+TGG | + | chr1.4:81797957-81797976 | MS.gene71587:intron | 35.0% |
| !! | CTTGTATGAAATAGTGGACA+TGG | + | chr1.4:81799083-81799102 | MS.gene71587:intron | 35.0% |
| !! | GTTGACACTGTTTTTCTGTT+CGG | - | chr1.4:81797643-81797662 | None:intergenic | 35.0% |
| !! | TTGACACTGTTTTTCTGTTC+GGG | - | chr1.4:81797642-81797661 | None:intergenic | 35.0% |
| !!! | GTTTTCCAATTGAGTTGGTT+AGG | - | chr1.4:81798603-81798622 | None:intergenic | 35.0% |
| AACAGCCAAATCAAGTTTGG+TGG | - | chr1.4:81797899-81797918 | None:intergenic | 40.0% | |
| AAGAGCGGAAAAAAATGCCA+CGG | - | chr1.4:81798403-81798422 | None:intergenic | 40.0% | |
| ACTATCACCTCCACAATATG+TGG | + | chr1.4:81799202-81799221 | MS.gene71587:CDS | 40.0% | |
| ACTTGGAGGAACATTCTTCT+TGG | + | chr1.4:81798353-81798372 | MS.gene71587:CDS | 40.0% | |
| AGAGCTTACGAAGTAAAGAG+CGG | - | chr1.4:81798418-81798437 | None:intergenic | 40.0% | |
| AGTGTCAACCAATGTTGAGA+GGG | + | chr1.4:81797654-81797673 | MS.gene71587:CDS | 40.0% | |
| ATCCTCAAGCAATGATGTAG+GGG | - | chr1.4:81797557-81797576 | None:intergenic | 40.0% | |
| CAGAACCTAACCAACTCAAT+TGG | + | chr1.4:81798595-81798614 | MS.gene71587:intron | 40.0% | |
| CTCTTCATCAACTCCATAGT+TGG | - | chr1.4:81797590-81797609 | None:intergenic | 40.0% | |
| GCATTGTTTGTTCTGCTCTA+AGG | + | chr1.4:81797815-81797834 | MS.gene71587:intron | 40.0% | |
| GGAACATTCTTCTTGGCATT+TGG | + | chr1.4:81798360-81798379 | MS.gene71587:CDS | 40.0% | |
| TACAGCTTACATGCTTCCTA+TGG | + | chr1.4:81798260-81798279 | MS.gene71587:intron | 40.0% | |
| TACTTAACACGCAAACGGTA+CGG | - | chr1.4:81798479-81798498 | None:intergenic | 40.0% | |
| TTGTCTTCCCATCTATCCTA+TGG | + | chr1.4:81798878-81798897 | MS.gene71587:intron | 40.0% | |
| ! | GTCAATAGAAGTTTAGCTCG+AGG | - | chr1.4:81798333-81798352 | None:intergenic | 40.0% |
| !! | CAACTATGGAGTTGATGAAG+AGG | + | chr1.4:81797588-81797607 | MS.gene71587:CDS | 40.0% |
| !! | TGATTTCTGGTGACTACCAT+AGG | - | chr1.4:81798279-81798298 | None:intergenic | 40.0% |
| CAGTGTCAACCAATGTTGAG+AGG | + | chr1.4:81797653-81797672 | MS.gene71587:CDS | 45.0% | |
| GCATAAGGGTCCACATATTG+TGG | - | chr1.4:81799215-81799234 | None:intergenic | 45.0% | |
| GGATATCTCAGATAGCTCCT+CGG | + | chr1.4:81799252-81799271 | MS.gene71587:CDS | 45.0% | |
| GTCTAGAGAATCAGAGGGAA+CGG | + | chr1.4:81797677-81797696 | MS.gene71587:CDS | 45.0% | |
| GTGTCAACCAATGTTGAGAG+GGG | + | chr1.4:81797655-81797674 | MS.gene71587:CDS | 45.0% | |
| TAAGGGTCCACATATTGTGG+AGG | - | chr1.4:81799212-81799231 | None:intergenic | 45.0% | |
| !!! | TTTTTTATATATATTATTGA+AGG | - | chr1.4:81797753-81797772 | None:intergenic | 5.0% |
| ACCGTTGATCCTCATAACCG+TGG | + | chr1.4:81798383-81798402 | MS.gene71587:CDS | 50.0% | |
| AGAGGGGTCTAGAGAATCAG+AGG | + | chr1.4:81797671-81797690 | MS.gene71587:CDS | 50.0% | |
| CTCTAGACCCCTCTCAACAT+TGG | - | chr1.4:81797665-81797684 | None:intergenic | 50.0% | |
| CTTCCGTCGTGGACAAAACT+TGG | - | chr1.4:81799173-81799192 | None:intergenic | 50.0% | |
| GAATCAGAGGGAACGGTCTA+TGG | + | chr1.4:81797684-81797703 | MS.gene71587:CDS | 50.0% | |
| GACCCCTACATCATTGCTTG+AGG | + | chr1.4:81797552-81797571 | MS.gene71587:CDS | 50.0% | |
| GAGGGGTCTAGAGAATCAGA+GGG | + | chr1.4:81797672-81797691 | MS.gene71587:CDS | 50.0% | |
| GCCACGGTTATGAGGATCAA+CGG | - | chr1.4:81798387-81798406 | None:intergenic | 50.0% | |
| GCTGAACAGTGAAGGTCTTG+AGG | + | chr1.4:81797708-81797727 | MS.gene71587:CDS | 50.0% | |
| ! | TCTATGGCGCTGAACAGTGA+AGG | + | chr1.4:81797700-81797719 | MS.gene71587:CDS | 50.0% |
| !! | GGACCAAGTTTTGTCCACGA+CGG | + | chr1.4:81799167-81799186 | MS.gene71587:CDS | 50.0% |
| GTGACATCTTGCTTCCGTCG+TGG | - | chr1.4:81799184-81799203 | None:intergenic | 55.0% | |
| ! | CGAGGTACCAAGCCCTGAAA+TGG | - | chr1.4:81798315-81798334 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 81797550 | 81799283 | 81797550 | ID=MS.gene71587 |
| chr1.4 | mRNA | 81797550 | 81799283 | 81797550 | ID=MS.gene71587.t1;Parent=MS.gene71587 |
| chr1.4 | exon | 81797550 | 81797729 | 81797550 | ID=MS.gene71587.t1.exon1;Parent=MS.gene71587.t1 |
| chr1.4 | CDS | 81797550 | 81797729 | 81797550 | ID=cds.MS.gene71587.t1;Parent=MS.gene71587.t1 |
| chr1.4 | exon | 81798321 | 81798428 | 81798321 | ID=MS.gene71587.t1.exon2;Parent=MS.gene71587.t1 |
| chr1.4 | CDS | 81798321 | 81798428 | 81798321 | ID=cds.MS.gene71587.t1;Parent=MS.gene71587.t1 |
| chr1.4 | exon | 81799161 | 81799283 | 81799161 | ID=MS.gene71587.t1.exon3;Parent=MS.gene71587.t1 |
| chr1.4 | CDS | 81799161 | 81799283 | 81799161 | ID=cds.MS.gene71587.t1;Parent=MS.gene71587.t1 |
| Gene Sequence |
| Protein sequence |
