Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052100.t1 | XP_003597359.1 | 97.5 | 120 | 3 | 0 | 1 | 120 | 3 | 122 | 1.80E-59 | 238.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052100.t1 | G7IHP7 | 97.5 | 120 | 3 | 0 | 1 | 120 | 3 | 122 | 1.3e-59 | 238.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049757 | MS.gene052100 | 0.807975 | 3.89E-50 | -1.69E-46 |
| MS.gene052100 | MS.gene05589 | 0.824927 | 6.19E-54 | -1.69E-46 |
| MS.gene052100 | MS.gene056387 | 0.842702 | 2.20E-58 | -1.69E-46 |
| MS.gene052100 | MS.gene056508 | 0.829438 | 5.15E-55 | -1.69E-46 |
| MS.gene052100 | MS.gene059667 | 0.801713 | 7.91E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene059717 | 0.809998 | 1.43E-50 | -1.69E-46 |
| MS.gene052100 | MS.gene06000 | 0.803001 | 4.29E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene061044 | -0.800375 | 1.48E-48 | -1.69E-46 |
| MS.gene052100 | MS.gene063561 | 0.816515 | 5.31E-52 | -1.69E-46 |
| MS.gene052100 | MS.gene066359 | -0.800201 | 1.61E-48 | -1.69E-46 |
| MS.gene052100 | MS.gene06750 | 0.835544 | 1.58E-56 | -1.69E-46 |
| MS.gene052100 | MS.gene06752 | 0.832031 | 1.19E-55 | -1.69E-46 |
| MS.gene052100 | MS.gene074380 | 0.831805 | 1.36E-55 | -1.69E-46 |
| MS.gene052100 | MS.gene08483 | -0.817063 | 4.00E-52 | -1.69E-46 |
| MS.gene052100 | MS.gene09137 | 0.808678 | 2.75E-50 | -1.69E-46 |
| MS.gene052100 | MS.gene21274 | -0.813174 | 2.93E-51 | -1.69E-46 |
| MS.gene052100 | MS.gene22750 | 0.817075 | 3.98E-52 | -1.69E-46 |
| MS.gene052100 | MS.gene22880 | 0.801156 | 1.03E-48 | -1.69E-46 |
| MS.gene052100 | MS.gene23185 | -0.8057 | 1.18E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene23404 | -0.800424 | 1.45E-48 | -1.69E-46 |
| MS.gene052100 | MS.gene23588 | -0.816603 | 5.08E-52 | -1.69E-46 |
| MS.gene052100 | MS.gene28137 | 0.824138 | 9.50E-54 | -1.69E-46 |
| MS.gene052100 | MS.gene28288 | 0.807745 | 4.35E-50 | -1.69E-46 |
| MS.gene052100 | MS.gene28334 | 0.850711 | 1.42E-60 | -1.69E-46 |
| MS.gene052100 | MS.gene28494 | 0.841675 | 4.12E-58 | -1.69E-46 |
| MS.gene052100 | MS.gene31137 | 0.924935 | 3.62E-90 | -1.69E-46 |
| MS.gene052100 | MS.gene38615 | -0.807393 | 5.17E-50 | -1.69E-46 |
| MS.gene052100 | MS.gene46880 | 0.854056 | 1.59E-61 | -1.69E-46 |
| MS.gene052100 | MS.gene51887 | 0.804112 | 2.53E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene53084 | 0.801222 | 9.97E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene54290 | -0.818877 | 1.56E-52 | -1.69E-46 |
| MS.gene052100 | MS.gene55291 | 0.804398 | 2.20E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene55731 | 0.80548 | 1.31E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene57998 | 0.807113 | 5.92E-50 | -1.69E-46 |
| MS.gene052100 | MS.gene58165 | 0.812716 | 3.68E-51 | -1.69E-46 |
| MS.gene052100 | MS.gene62884 | -0.812692 | 3.73E-51 | -1.69E-46 |
| MS.gene052100 | MS.gene64829 | 0.804255 | 2.36E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene67597 | 0.811934 | 5.46E-51 | -1.69E-46 |
| MS.gene052100 | MS.gene70306 | 0.820537 | 6.51E-53 | -1.69E-46 |
| MS.gene052100 | MS.gene70327 | 0.85179 | 7.06E-61 | -1.69E-46 |
| MS.gene052100 | MS.gene70461 | 0.834472 | 2.94E-56 | -1.69E-46 |
| MS.gene052100 | MS.gene70601 | 0.80256 | 5.29E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene71300 | 0.836623 | 8.40E-57 | -1.69E-46 |
| MS.gene052100 | MS.gene71587 | 0.826851 | 2.16E-54 | -1.69E-46 |
| MS.gene052100 | MS.gene71621 | 0.812114 | 4.99E-51 | -1.69E-46 |
| MS.gene052100 | MS.gene72923 | -0.806815 | 6.85E-50 | -1.69E-46 |
| MS.gene052100 | MS.gene74628 | 0.815664 | 8.23E-52 | -1.69E-46 |
| MS.gene052100 | MS.gene75179 | -0.806381 | 8.46E-50 | -1.69E-46 |
| MS.gene052100 | MS.gene76802 | 0.806743 | 7.09E-50 | -1.69E-46 |
| MS.gene052100 | MS.gene78467 | 0.894847 | 1.68E-75 | -1.69E-46 |
| MS.gene052100 | MS.gene78560 | -0.802535 | 5.36E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene81988 | 0.830439 | 2.93E-55 | -1.69E-46 |
| MS.gene052100 | MS.gene84140 | 0.813513 | 2.46E-51 | -1.69E-46 |
| MS.gene052100 | MS.gene87400 | 0.846673 | 1.87E-59 | -1.69E-46 |
| MS.gene052100 | MS.gene88640 | 0.804408 | 2.19E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene88781 | 0.812333 | 4.47E-51 | -1.69E-46 |
| MS.gene052100 | MS.gene89722 | 0.810465 | 1.14E-50 | -1.69E-46 |
| MS.gene052100 | MS.gene90842 | -0.829552 | 4.83E-55 | -1.69E-46 |
| MS.gene052100 | MS.gene90966 | -0.813108 | 3.02E-51 | -1.69E-46 |
| MS.gene052100 | MS.gene91347 | 0.825237 | 5.23E-54 | -1.69E-46 |
| MS.gene052100 | MS.gene93738 | 0.814174 | 1.76E-51 | -1.69E-46 |
| MS.gene052100 | MS.gene95699 | 0.828644 | 8.02E-55 | -1.69E-46 |
| MS.gene052100 | MS.gene96193 | -0.800712 | 1.27E-48 | -1.69E-46 |
| MS.gene052100 | MS.gene98762 | 0.815575 | 8.62E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052100.t1 | MTR_2g097230 | 97.500 | 120 | 3 | 0 | 1 | 120 | 3 | 122 | 1.65e-83 | 246 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052100.t1 | AT5G59400 | 59.200 | 125 | 42 | 2 | 5 | 120 | 6 | 130 | 2.58e-43 | 143 |
| MS.gene052100.t1 | AT5G59400 | 59.200 | 125 | 42 | 2 | 5 | 120 | 6 | 130 | 1.33e-42 | 142 |
Find 33 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAACGGTTGTTTCTGATTT+TGG | 0.096153 | 2.3:+5652068 | None:intergenic |
| AAAGAGCTTCAAGGGTTTCT+TGG | 0.284671 | 2.3:+5651950 | None:intergenic |
| TTTCATCTTCCTTCTCTCTT+CGG | 0.366302 | 2.3:-5652044 | MS.gene052100:CDS |
| CCTTGAAGCTCTTTATTCTC+AGG | 0.389886 | 2.3:-5651941 | MS.gene052100:intron |
| AAGAGCTTCAAGGGTTTCTT+GGG | 0.410172 | 2.3:+5651951 | None:intergenic |
| CCTGAGAATAAAGAGCTTCA+AGG | 0.416387 | 2.3:+5651941 | None:intergenic |
| AGCAACAGCAGCACCGCTTT+AGG | 0.444700 | 2.3:-5652114 | MS.gene052100:CDS |
| GGTAACAGTTGAAGCTTAAG+TGG | 0.481788 | 2.3:-5651587 | MS.gene052100:intron |
| CAGTTGAAGCTTAAGTGGTA+TGG | 0.493314 | 2.3:-5651582 | MS.gene052100:CDS |
| CTGAGAATAAAGAGCTTCAA+GGG | 0.499082 | 2.3:+5651942 | None:intergenic |
| GGTCCATCTTGTATATATGT+TGG | 0.512587 | 2.3:-5651991 | MS.gene052100:CDS |
| AGGATACTTCACAACTGACT+TGG | 0.542875 | 2.3:+5651557 | None:intergenic |
| TCTTGGGTGGCAGTTTGAAG+AGG | 0.543205 | 2.3:+5651967 | None:intergenic |
| ACCTGCGACGACCGTCCTCA+TGG | 0.549456 | 2.3:-5652012 | MS.gene052100:CDS |
| AACATGTCATCAACTATCAA+AGG | 0.550328 | 2.3:+5651707 | None:intergenic |
| GGCAGAGATCCGAAGAGAGA+AGG | 0.551019 | 2.3:+5652035 | None:intergenic |
| ACCATGAGGACGGTCGTCGC+AGG | 0.551432 | 2.3:+5652011 | None:intergenic |
| GCAGCACCGCTTTAGGTGGA+TGG | 0.553063 | 2.3:-5652107 | MS.gene052100:CDS |
| GGACCAACATATATACAAGA+TGG | 0.557422 | 2.3:+5651988 | None:intergenic |
| AGCTTCAAGGGTTTCTTGGG+TGG | 0.559365 | 2.3:+5651954 | None:intergenic |
| CACCGTCCATCCACCTAAAG+CGG | 0.577414 | 2.3:+5652101 | None:intergenic |
| CACCGCTTTAGGTGGATGGA+CGG | 0.580229 | 2.3:-5652103 | MS.gene052100:CDS |
| ATGAGGACGGTCGTCGCAGG+TGG | 0.602794 | 2.3:+5652014 | None:intergenic |
| AACAGCAGCACCGCTTTAGG+TGG | 0.608229 | 2.3:-5652111 | MS.gene052100:CDS |
| ATCCTCGTTGCAGTATCAGA+AGG | 0.615303 | 2.3:-5651539 | MS.gene052100:CDS |
| AATCACCTCAGCATCAGCAT+AGG | 0.620614 | 2.3:+5651509 | None:intergenic |
| TGACATGTTTGATAGAGTAG+AGG | 0.624434 | 2.3:-5651693 | MS.gene052100:intron |
| GCAAGGGATGCATATTACAA+AGG | 0.629849 | 2.3:-5651734 | MS.gene052100:CDS |
| TACAAGATGGACCATGAGGA+CGG | 0.641757 | 2.3:+5652001 | None:intergenic |
| ATCAACCTATGCTGATGCTG+AGG | 0.651620 | 2.3:-5651514 | MS.gene052100:CDS |
| AGAAGGAAGATGAAACAGAA+CGG | 0.670572 | 2.3:+5652052 | None:intergenic |
| TATATACAAGATGGACCATG+AGG | 0.716840 | 2.3:+5651997 | None:intergenic |
| TGCCTTCTGATACTGCAACG+AGG | 0.764708 | 2.3:+5651537 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCTTATGTTATTCATATTA+TGG | - | chr2.3:5652012-5652031 | MS.gene052100:CDS | 15.0% |
| !!! | ATTGAGAATTATTTTAAACA+TGG | - | chr2.3:5651842-5651861 | MS.gene052100:intron | 15.0% |
| !! | GAATAACATAAGATTAAGAT+CGG | + | chr2.3:5652006-5652025 | None:intergenic | 20.0% |
| !!! | GAATTATTTTAAACATGGCA+TGG | - | chr2.3:5651847-5651866 | MS.gene052100:intron | 25.0% |
| AACATGTCATCAACTATCAA+AGG | + | chr2.3:5651916-5651935 | None:intergenic | 30.0% | |
| ! | AAGAAGTTAAGAAGCATATG+TGG | + | chr2.3:5651974-5651993 | None:intergenic | 30.0% |
| AGAAGGAAGATGAAACAGAA+CGG | + | chr2.3:5651571-5651590 | None:intergenic | 35.0% | |
| CTGAGAATAAAGAGCTTCAA+GGG | + | chr2.3:5651681-5651700 | None:intergenic | 35.0% | |
| GCGCTTATGCTTAATTAAGT+TGG | - | chr2.3:5651769-5651788 | MS.gene052100:intron | 35.0% | |
| GGACCAACATATATACAAGA+TGG | + | chr2.3:5651635-5651654 | None:intergenic | 35.0% | |
| GGTCCATCTTGTATATATGT+TGG | - | chr2.3:5651629-5651648 | MS.gene052100:intron | 35.0% | |
| TATATACAAGATGGACCATG+AGG | + | chr2.3:5651626-5651645 | None:intergenic | 35.0% | |
| TGACATGTTTGATAGAGTAG+AGG | - | chr2.3:5651927-5651946 | MS.gene052100:intron | 35.0% | |
| TGATCTTTGCTAATAGGCAA+GGG | - | chr2.3:5651870-5651889 | MS.gene052100:intron | 35.0% | |
| TTTCATCTTCCTTCTCTCTT+CGG | - | chr2.3:5651576-5651595 | MS.gene052100:CDS | 35.0% | |
| ! | AAGAAGCATATGTGGTGAAT+TGG | + | chr2.3:5651966-5651985 | None:intergenic | 35.0% |
| ! | AGAAGCATATGTGGTGAATT+GGG | + | chr2.3:5651965-5651984 | None:intergenic | 35.0% |
| !!! | AGAACGGTTGTTTCTGATTT+TGG | + | chr2.3:5651555-5651574 | None:intergenic | 35.0% |
| AAAGAGCTTCAAGGGTTTCT+TGG | + | chr2.3:5651673-5651692 | None:intergenic | 40.0% | |
| AAGAGCTTCAAGGGTTTCTT+GGG | + | chr2.3:5651672-5651691 | None:intergenic | 40.0% | |
| AAGCATATGTGGTGAATTGG+GGG | + | chr2.3:5651963-5651982 | None:intergenic | 40.0% | |
| AGGATACTTCACAACTGACT+TGG | + | chr2.3:5652066-5652085 | None:intergenic | 40.0% | |
| CAGTTGAAGCTTAAGTGGTA+TGG | - | chr2.3:5652038-5652057 | MS.gene052100:CDS | 40.0% | |
| CCTGAGAATAAAGAGCTTCA+AGG | + | chr2.3:5651682-5651701 | None:intergenic | 40.0% | |
| CCTTGAAGCTCTTTATTCTC+AGG | - | chr2.3:5651679-5651698 | MS.gene052100:intron | 40.0% | |
| GAAGCATATGTGGTGAATTG+GGG | + | chr2.3:5651964-5651983 | None:intergenic | 40.0% | |
| GCAAGGGATGCATATTACAA+AGG | - | chr2.3:5651886-5651905 | MS.gene052100:intron | 40.0% | |
| GCATGGTGATCTTTGCTAAT+AGG | - | chr2.3:5651864-5651883 | MS.gene052100:intron | 40.0% | |
| GGTAACAGTTGAAGCTTAAG+TGG | - | chr2.3:5652033-5652052 | MS.gene052100:CDS | 40.0% | |
| GTGATCTTTGCTAATAGGCA+AGG | - | chr2.3:5651869-5651888 | MS.gene052100:intron | 40.0% | |
| AATCACCTCAGCATCAGCAT+AGG | + | chr2.3:5652114-5652133 | None:intergenic | 45.0% | |
| ATCAACCTATGCTGATGCTG+AGG | - | chr2.3:5652106-5652125 | MS.gene052100:CDS | 45.0% | |
| ATCCTCGTTGCAGTATCAGA+AGG | - | chr2.3:5652081-5652100 | MS.gene052100:CDS | 45.0% | |
| TACAAGATGGACCATGAGGA+CGG | + | chr2.3:5651622-5651641 | None:intergenic | 45.0% | |
| AGCTTCAAGGGTTTCTTGGG+TGG | + | chr2.3:5651669-5651688 | None:intergenic | 50.0% | |
| TCTTGGGTGGCAGTTTGAAG+AGG | + | chr2.3:5651656-5651675 | None:intergenic | 50.0% | |
| TGCCTTCTGATACTGCAACG+AGG | + | chr2.3:5652086-5652105 | None:intergenic | 50.0% | |
| AGCAACAGCAGCACCGCTTT+AGG | - | chr2.3:5651506-5651525 | MS.gene052100:CDS | 55.0% | |
| CACCGTCCATCCACCTAAAG+CGG | + | chr2.3:5651522-5651541 | None:intergenic | 55.0% | |
| GGCAGAGATCCGAAGAGAGA+AGG | + | chr2.3:5651588-5651607 | None:intergenic | 55.0% | |
| ! | AACAGCAGCACCGCTTTAGG+TGG | - | chr2.3:5651509-5651528 | MS.gene052100:CDS | 55.0% |
| ! | CACCGCTTTAGGTGGATGGA+CGG | - | chr2.3:5651517-5651536 | MS.gene052100:CDS | 55.0% |
| ! | GCAGCACCGCTTTAGGTGGA+TGG | - | chr2.3:5651513-5651532 | MS.gene052100:CDS | 60.0% |
| ACCATGAGGACGGTCGTCGC+AGG | + | chr2.3:5651612-5651631 | None:intergenic | 65.0% | |
| ACCTGCGACGACCGTCCTCA+TGG | - | chr2.3:5651608-5651627 | MS.gene052100:intron | 65.0% | |
| ATGAGGACGGTCGTCGCAGG+TGG | + | chr2.3:5651609-5651628 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 5651491 | 5652151 | 5651491 | ID=MS.gene052100 |
| chr2.3 | mRNA | 5651491 | 5652151 | 5651491 | ID=MS.gene052100.t1;Parent=MS.gene052100 |
| chr2.3 | exon | 5651942 | 5652151 | 5651942 | ID=MS.gene052100.t1.exon1;Parent=MS.gene052100.t1 |
| chr2.3 | CDS | 5651942 | 5652151 | 5651942 | ID=cds.MS.gene052100.t1;Parent=MS.gene052100.t1 |
| chr2.3 | exon | 5651694 | 5651756 | 5651694 | ID=MS.gene052100.t1.exon2;Parent=MS.gene052100.t1 |
| chr2.3 | CDS | 5651694 | 5651756 | 5651694 | ID=cds.MS.gene052100.t1;Parent=MS.gene052100.t1 |
| chr2.3 | exon | 5651491 | 5651601 | 5651491 | ID=MS.gene052100.t1.exon3;Parent=MS.gene052100.t1 |
| chr2.3 | CDS | 5651491 | 5651601 | 5651491 | ID=cds.MS.gene052100.t1;Parent=MS.gene052100.t1 |
| Gene Sequence |
| Protein sequence |
