AlfalfaGEDB | MS.gene049757

M. sativa cultivar XinJiangDaYe / MS.gene049757

KEGG: K13071
GO: GO:0003674 GO:0003824 GO:0004497 GO:0005488 GO:0008150 GO:0008152 GO:0010277 GO:0016491 GO:0016701 GO:0016703 GO:0048037 GO:0051536 GO:0051537 GO:0051540 GO:0055114
PFAM: PF00355.29 PF08417.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049757.t1	XP_003606324.1	97.8	545	9	1	1	545	1	542	0.00E+00	1109.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049757.t1	Q9FYC2	69.6	526	146	5	30	545	16	537	1.0e-224	780.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049757.t1	G7JM88	97.8	545	9	1	1	545	1	542	0.0e+00	1109.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049757	MS.gene050133	-0.803848	2.87E-49	-1.69E-46
MS.gene049757	MS.gene050553	0.832637	8.44E-56	-1.69E-46
MS.gene049757	MS.gene051521	0.819946	8.89E-53	-1.69E-46
MS.gene049757	MS.gene052100	0.807975	3.89E-50	-1.69E-46
MS.gene049757	MS.gene052414	0.800474	1.42E-48	-1.69E-46
MS.gene049757	MS.gene052469	0.837677	4.51E-57	-1.69E-46
MS.gene049757	MS.gene053169	0.809351	1.98E-50	-1.69E-46
MS.gene049757	MS.gene053889	0.826216	3.06E-54	-1.69E-46
MS.gene049757	MS.gene055539	0.83456	2.79E-56	-1.69E-46
MS.gene049757	MS.gene055741	0.846656	1.89E-59	-1.69E-46
MS.gene049757	MS.gene055744	0.850492	1.64E-60	-1.69E-46
MS.gene049757	MS.gene056233	0.814373	1.59E-51	-1.69E-46
MS.gene049757	MS.gene056508	0.822791	1.96E-53	-1.69E-46
MS.gene049757	MS.gene057019	0.815025	1.14E-51	-1.69E-46
MS.gene049757	MS.gene057396	0.816047	6.76E-52	-1.69E-46
MS.gene049757	MS.gene058090	0.800782	1.23E-48	-1.69E-46
MS.gene049757	MS.gene058448	0.817361	3.43E-52	-1.69E-46
MS.gene049757	MS.gene059717	0.844109	9.26E-59	-1.69E-46
MS.gene049757	MS.gene059868	0.809075	2.26E-50	-1.69E-46
MS.gene049757	MS.gene059946	0.800031	1.74E-48	-1.69E-46
MS.gene049757	MS.gene060372	0.833758	4.44E-56	-1.69E-46
MS.gene049757	MS.gene06260	0.83781	4.17E-57	-1.69E-46
MS.gene049757	MS.gene065168	0.820617	6.24E-53	-1.69E-46
MS.gene049757	MS.gene066406	0.864149	1.50E-64	-1.69E-46
MS.gene049757	MS.gene06923	0.812524	4.06E-51	-1.69E-46
MS.gene049757	MS.gene069383	0.801063	1.07E-48	-1.69E-46
MS.gene049757	MS.gene069717	0.805391	1.37E-49	-1.69E-46
MS.gene049757	MS.gene071722	0.807116	5.91E-50	-1.69E-46
MS.gene049757	MS.gene072070	0.852135	5.63E-61	-1.69E-46
MS.gene049757	MS.gene072935	0.814949	1.19E-51	-1.69E-46
MS.gene049757	MS.gene073427	0.823468	1.36E-53	-1.69E-46
MS.gene049757	MS.gene073486	0.854933	8.85E-62	-1.69E-46
MS.gene049757	MS.gene08083	0.824252	8.93E-54	-1.69E-46
MS.gene049757	MS.gene08634	-0.822823	1.93E-53	-1.69E-46
MS.gene049757	MS.gene09137	0.946075	9.20E-105	-1.69E-46
MS.gene049757	MS.gene09570	0.815361	9.62E-52	-1.69E-46
MS.gene049757	MS.gene09765	0.856547	2.99E-62	-1.69E-46
MS.gene049757	MS.gene09777	0.875724	2.46E-68	-1.69E-46
MS.gene049757	MS.gene21013	0.804622	1.98E-49	-1.69E-46
MS.gene049757	MS.gene22880	0.834011	3.83E-56	-1.69E-46
MS.gene049757	MS.gene22896	0.803748	3.01E-49	-1.69E-46
MS.gene049757	MS.gene22898	0.836381	9.67E-57	-1.69E-46
MS.gene049757	MS.gene23184	-0.81434	1.62E-51	-1.69E-46
MS.gene049757	MS.gene23185	-0.820866	5.47E-53	-1.69E-46
MS.gene049757	MS.gene23404	-0.824124	9.56E-54	-1.69E-46
MS.gene049757	MS.gene23588	-0.819918	9.02E-53	-1.69E-46
MS.gene049757	MS.gene25670	0.810938	8.99E-51	-1.69E-46
MS.gene049757	MS.gene25929	0.801258	9.80E-49	-1.69E-46
MS.gene049757	MS.gene30953	0.811892	5.58E-51	-1.69E-46
MS.gene049757	MS.gene31137	0.824754	6.80E-54	-1.69E-46
MS.gene049757	MS.gene32713	0.844247	8.51E-59	-1.69E-46
MS.gene049757	MS.gene33076	0.821481	3.95E-53	-1.69E-46
MS.gene049757	MS.gene34188	0.803494	3.39E-49	-1.69E-46
MS.gene049757	MS.gene34394	0.804572	2.03E-49	-1.69E-46
MS.gene049757	MS.gene35332	0.807918	4.00E-50	-1.69E-46
MS.gene049757	MS.gene35984	0.810778	9.73E-51	-1.69E-46
MS.gene049757	MS.gene39277	0.801239	9.89E-49	-1.69E-46
MS.gene049757	MS.gene39366	0.821202	4.58E-53	-1.69E-46
MS.gene049757	MS.gene41649	0.842821	2.05E-58	-1.69E-46
MS.gene049757	MS.gene42746	0.804501	2.10E-49	-1.69E-46
MS.gene049757	MS.gene43797	0.836493	9.06E-57	-1.69E-46
MS.gene049757	MS.gene44893	0.84087	6.70E-58	-1.69E-46
MS.gene049757	MS.gene48392	0.813309	2.73E-51	-1.69E-46
MS.gene049757	MS.gene51034	0.833905	4.08E-56	-1.69E-46
MS.gene049757	MS.gene52883	0.82431	8.65E-54	-1.69E-46
MS.gene049757	MS.gene55820	0.807733	4.38E-50	-1.69E-46
MS.gene049757	MS.gene59687	0.836467	9.20E-57	-1.69E-46
MS.gene049757	MS.gene60748	0.8262	3.09E-54	-1.69E-46
MS.gene049757	MS.gene61068	0.800458	1.43E-48	-1.69E-46
MS.gene049757	MS.gene61373	0.803538	3.32E-49	-1.69E-46
MS.gene049757	MS.gene61544	0.800391	1.47E-48	-1.69E-46
MS.gene049757	MS.gene62091	0.804157	2.47E-49	-1.69E-46
MS.gene049757	MS.gene65435	0.806547	7.80E-50	-1.69E-46
MS.gene049757	MS.gene68943	0.829023	6.49E-55	-1.69E-46
MS.gene049757	MS.gene69343	0.808172	3.53E-50	-1.69E-46
MS.gene049757	MS.gene70306	0.812347	4.44E-51	-1.69E-46
MS.gene049757	MS.gene70461	0.814079	1.85E-51	-1.69E-46
MS.gene049757	MS.gene71300	0.805476	1.31E-49	-1.69E-46
MS.gene049757	MS.gene71587	0.80219	6.31E-49	-1.69E-46
MS.gene049757	MS.gene73446	0.809513	1.82E-50	-1.69E-46
MS.gene049757	MS.gene75001	0.810482	1.13E-50	-1.69E-46
MS.gene049757	MS.gene75660	0.847743	9.53E-60	-1.69E-46
MS.gene049757	MS.gene76141	0.809914	1.50E-50	-1.69E-46
MS.gene049757	MS.gene76802	0.828975	6.67E-55	-1.69E-46
MS.gene049757	MS.gene77541	0.850381	1.76E-60	-1.69E-46
MS.gene049757	MS.gene78431	0.8184	2.00E-52	-1.69E-46
MS.gene049757	MS.gene78467	0.845398	4.16E-59	-1.69E-46
MS.gene049757	MS.gene78643	0.851348	9.41E-61	-1.69E-46
MS.gene049757	MS.gene79020	0.807114	5.92E-50	-1.69E-46
MS.gene049757	MS.gene79903	0.837776	4.26E-57	-1.69E-46
MS.gene049757	MS.gene79962	-0.80255	5.32E-49	-1.69E-46
MS.gene049757	MS.gene80172	0.856671	2.75E-62	-1.69E-46
MS.gene049757	MS.gene84140	0.817309	3.53E-52	-1.69E-46
MS.gene049757	MS.gene84200	0.808517	2.98E-50	-1.69E-46
MS.gene049757	MS.gene84544	0.873701	1.20E-67	-1.69E-46
MS.gene049757	MS.gene85095	0.843552	1.31E-58	-1.69E-46
MS.gene049757	MS.gene86095	0.808123	3.62E-50	-1.69E-46
MS.gene049757	MS.gene88498	0.848176	7.24E-60	-1.69E-46
MS.gene049757	MS.gene88781	0.832941	7.09E-56	-1.69E-46
MS.gene049757	MS.gene88785	0.819538	1.10E-52	-1.69E-46
MS.gene049757	MS.gene91302	0.831273	1.83E-55	-1.69E-46
MS.gene049757	MS.gene91347	0.816229	6.16E-52	-1.69E-46
MS.gene049757	MS.gene94682	0.809579	1.76E-50	-1.69E-46
MS.gene049757	MS.gene95699	0.843669	1.22E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049757.t1	MTR_4g057200	97.798	545	9	1	1	545	1	542	0.0	1107
MS.gene049757.t1	MTR_8g085840	35.874	538	285	22	33	542	22	527	1.60e-91	290
MS.gene049757.t1	MTR_8g085790	36.626	486	262	18	79	542	41	502	2.51e-90	287
MS.gene049757.t1	MTR_8g085820	35.992	489	262	18	79	542	41	503	3.41e-88	281
MS.gene049757.t1	MTR_8g085790	37.565	386	201	14	33	399	22	386	1.44e-69	229
MS.gene049757.t1	MTR_3g105820	28.931	477	276	17	81	523	84	531	2.52e-52	187

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049757.t1	AT3G44880	69.582	526	146	5	30	545	16	537	0.0	775
MS.gene049757.t1	AT4G25650	33.333	486	274	12	87	544	73	536	2.32e-82	267
MS.gene049757.t1	AT4G25650	32.024	509	273	13	87	544	73	559	6.60e-79	258
MS.gene049757.t1	AT2G24820	27.866	567	323	20	15	535	7	533	4.40e-53	189

Find 126 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTTCTCCTGTTTCTGTTTC+TGG	-0.047754	4.2:+46576996	None:intergenic
GCCTACGCCGTTTCAGCTTC+TGG	0.120106	4.2:-46576791	MS.gene049757:CDS
CACTTCTAACTGTTACTAAA+AGG	0.221780	4.2:+46576974	None:intergenic
ATAACATGGTGCAATAAATT+TGG	0.238122	4.2:+46569831	None:intergenic
CTCAGGGATTGCTCTTTGTT+TGG	0.265240	4.2:-46571801	MS.gene049757:CDS
TTTCTCCTGTTTCTGTTTCT+GGG	0.271424	4.2:+46576997	None:intergenic
CTGCTCTTGCCGAACCATTC+AGG	0.272155	4.2:+46568822	None:intergenic
ATATCCATATTACCCATTTC+TGG	0.295421	4.2:+46569431	None:intergenic
GATGAAAAGAGAAACGGTTT+TGG	0.304587	4.2:+46577060	None:intergenic
AGATCTTCAATCAAAGAAAC+TGG	0.307795	4.2:+46576826	None:intergenic
GTTTCCTACCTTGGTGTCTC+AGG	0.314846	4.2:-46571818	MS.gene049757:CDS
ATTTCCACTCCATAGGTTAC+AGG	0.323502	4.2:-46569949	MS.gene049757:intron
TACTCAGCTGCGCATAGTTT+TGG	0.327030	4.2:-46568518	MS.gene049757:CDS
CCTACGCCGTTTCAGCTTCT+GGG	0.330171	4.2:-46576790	MS.gene049757:CDS
TTCTTCCAGCTCACGGTGCC+AGG	0.334083	4.2:-46569346	MS.gene049757:CDS
CCGTGGTTGGTGACCAGAAA+TGG	0.334810	4.2:-46569444	MS.gene049757:CDS
TTTGGCCTGATGAGAATGTT+TGG	0.339720	4.2:-46571783	MS.gene049757:CDS
GATCCAACAGCACGCGCTTC+CGG	0.340404	4.2:+46571862	None:intergenic
AGTTTCTTTGATTGAAGATC+TGG	0.343549	4.2:-46576824	MS.gene049757:CDS
TGCTACTAGGTTTCCTACCT+TGG	0.353927	4.2:-46571827	MS.gene049757:CDS
GTGACCAGAAATGGGTAATA+TGG	0.353988	4.2:-46569435	MS.gene049757:CDS
GAAGGAAGGATAGATGAAAA+TGG	0.368399	4.2:-46571967	MS.gene049757:CDS
GTTAATGTCACCGCCATTCT+CGG	0.368858	4.2:+46568938	None:intergenic
TTGTGTTGGCATTTAGAAAT+TGG	0.373510	4.2:-46568869	MS.gene049757:CDS
GTCACCAACCACGGGGAGTT+TGG	0.380037	4.2:+46569453	None:intergenic
AGATGGATTCACGTGGTTCA+TGG	0.404907	4.2:-46569894	MS.gene049757:CDS
TCACGGTGCCAGGGCCTGCC+TGG	0.405008	4.2:-46569336	MS.gene049757:CDS
GCACGCGCTTCCGGACCTTC+AGG	0.406540	4.2:+46571871	None:intergenic
CAGGCCCTGGCACCGTGAGC+TGG	0.407256	4.2:+46569341	None:intergenic
TTCTCCCAAACATTCTCATC+AGG	0.416066	4.2:+46571778	None:intergenic
GGTCCGGAAGCGCGTGCTGT+TGG	0.422340	4.2:-46571865	MS.gene049757:CDS
CTCCTAGAGCTTGTGCTACT+AGG	0.425826	4.2:-46571840	MS.gene049757:CDS
CATCTTGAATGGAAGAGGTT+TGG	0.427132	4.2:+46569912	None:intergenic
ACTTGCCACCAGGCAGGCCC+TGG	0.430231	4.2:+46569328	None:intergenic
TGGGTCGTGAAATTGTGCTT+TGG	0.438370	4.2:-46576771	MS.gene049757:CDS
AGCTTGTAAAGGAGCTTATG+AGG	0.440308	4.2:-46568614	MS.gene049757:CDS
TTGATAAGGCTAATTCTCAA+TGG	0.441751	4.2:-46576747	MS.gene049757:CDS
TCAGGCTTCACCTGAAGGTC+CGG	0.443398	4.2:-46571881	MS.gene049757:CDS
CAAGACCCAGAAACAGAAAC+AGG	0.444909	4.2:-46577002	MS.gene049757:CDS
GATGGATTCACGTGGTTCAT+GGG	0.447972	4.2:-46569893	MS.gene049757:CDS
ATCCCTCAGGCTTCACCTGA+AGG	0.455756	4.2:-46571886	MS.gene049757:CDS
TTTCCTACCTTGGTGTCTCA+GGG	0.456423	4.2:-46571817	MS.gene049757:CDS
AATTGTGCTTTGGTTTGATA+AGG	0.463189	4.2:-46576761	MS.gene049757:CDS
TTGGCCTGATGAGAATGTTT+GGG	0.464179	4.2:-46571782	MS.gene049757:CDS
TGATAAGGCTAATTCTCAAT+GGG	0.464457	4.2:-46576746	MS.gene049757:CDS
TGAAACGGCGTAGGCAAACT+AGG	0.465653	4.2:+46576799	None:intergenic
AACTTGCAGTGTTCTTATCA+TGG	0.471539	4.2:-46571943	MS.gene049757:CDS
TAAACAAATCATTACATTGG+AGG	0.472678	4.2:+46571739	None:intergenic
CCACGTGAATCCATCTTGAA+TGG	0.476840	4.2:+46569901	None:intergenic
TGTCTCAGGTGGTTCCTAGA+TGG	0.480851	4.2:-46569043	MS.gene049757:intron
ACACAACTGTTGCACCAGTC+AGG	0.489551	4.2:+46568569	None:intergenic
CGTGGTTGGTGACCAGAAAT+GGG	0.494565	4.2:-46569443	MS.gene049757:CDS
GTGAAGGATGAAAAGAGAAA+CGG	0.495635	4.2:+46577054	None:intergenic
ATGTTCACCGTCGCAAACTC+TGG	0.495962	4.2:+46571390	None:intergenic
CGAGTCTTACCAGGTGCCAT+GGG	0.496305	4.2:+46569394	None:intergenic
AACCGTACTATCAAAACGTC+AGG	0.497409	4.2:-46568781	MS.gene049757:intron
AATGGCCAACCTGAATGGTT+CGG	0.505921	4.2:-46568831	MS.gene049757:CDS
GAATCCATCTTGAATGGAAG+AGG	0.506455	4.2:+46569907	None:intergenic
TTCAATGTCCCCATGGCACC+TGG	0.510022	4.2:-46569403	MS.gene049757:CDS
TCGAAATTTCTTCCAGCTCA+CGG	0.516385	4.2:-46569353	MS.gene049757:CDS
GCGAGTCTTACCAGGTGCCA+TGG	0.519997	4.2:+46569393	None:intergenic
ACGGCAATGGCCAACCTGAA+TGG	0.522604	4.2:-46568836	MS.gene049757:CDS
TGCAGTGTTCTTATCATGGG+TGG	0.523912	4.2:-46571939	MS.gene049757:CDS
CTAAAGTGTTCAGCTTGTAA+AGG	0.524879	4.2:-46568625	MS.gene049757:CDS
TGGCTGAGGCGACACGGCAA+TGG	0.525095	4.2:-46568849	MS.gene049757:CDS
AGGTGCCATGGGGACATTGA+AGG	0.525388	4.2:+46569405	None:intergenic
GTACGGTTGAAGGCAATGGC+TGG	0.525732	4.2:+46568796	None:intergenic
TCACGACCCAGAAGCTGAAA+CGG	0.528461	4.2:+46576784	None:intergenic
ACTTGCAGTGTTCTTATCAT+GGG	0.533069	4.2:-46571942	MS.gene049757:CDS
CATTTCTGGTCACCAACCAC+GGG	0.533924	4.2:+46569445	None:intergenic
CTACGGCTATGATACTCTCA+TGG	0.535693	4.2:-46571352	MS.gene049757:CDS
CAAACCTCTTCCATTCAAGA+TGG	0.540474	4.2:-46569911	MS.gene049757:CDS
CGGTGCCAGGGCCTGCCTGG+TGG	0.541920	4.2:-46569333	MS.gene049757:CDS
ACGATGGAGCGAGTCTTACC+AGG	0.542041	4.2:+46569385	None:intergenic
TTTCTCGCAGAAACCGAGAA+TGG	0.544480	4.2:-46568951	MS.gene049757:CDS
GTCCAATGTTCATACCATCT+AGG	0.546736	4.2:+46569029	None:intergenic
GGTGACATGATTGTACTTCA+AGG	0.548561	4.2:-46568987	MS.gene049757:CDS
TCTTCCAGCTCACGGTGCCA+GGG	0.550242	4.2:-46569345	MS.gene049757:CDS
ATGCGCAGCTGAGTATCTGA+TGG	0.558491	4.2:+46568526	None:intergenic
GATAGTACGGTTGAAGGCAA+TGG	0.559294	4.2:+46568792	None:intergenic
CTTATGAGGGATTCCAAACA+TGG	0.560004	4.2:-46568600	MS.gene049757:CDS
AGGTTGCACTGTGATCCCTC+AGG	0.562050	4.2:-46571899	MS.gene049757:CDS
AATCTTACTTGCCACCAGGC+AGG	0.562524	4.2:+46569322	None:intergenic
TAAATTTGGAACTGATCCGC+GGG	0.568789	4.2:+46569845	None:intergenic
ATCGGCCTGTGTTGGTGTGA+AGG	0.569741	4.2:+46568896	None:intergenic
AACCTAGTAGCACAAGCTCT+AGG	0.570794	4.2:+46571838	None:intergenic
CCATTTCTGGTCACCAACCA+CGG	0.571640	4.2:+46569444	None:intergenic
GACCTTCAGGTGAAGCCTGA+GGG	0.571686	4.2:+46571884	None:intergenic
ACAAAACGATCGGCCTGTGT+TGG	0.573172	4.2:+46568888	None:intergenic
TTCCTAGATGGTATGAACAT+TGG	0.578101	4.2:-46569031	MS.gene049757:CDS
GCTTGTAAAGGAGCTTATGA+GGG	0.580240	4.2:-46568613	MS.gene049757:CDS
AAAACTACACTTACGGACAA+AGG	0.584684	4.2:+46576688	None:intergenic
ATGGATTCACGTGGTTCATG+GGG	0.586611	4.2:-46569892	MS.gene049757:CDS
TGGCATTTAGAAATTGGCTG+AGG	0.593473	4.2:-46568863	MS.gene049757:CDS
TCTTGCCGAACCATTCAGGT+TGG	0.593673	4.2:+46568826	None:intergenic
AGAAATTGGCTGAGGCGACA+CGG	0.601844	4.2:-46568855	MS.gene049757:CDS
GATACCAAACTCCCCGTGGT+TGG	0.603630	4.2:-46569457	MS.gene049757:CDS
ACATGGCAGAAAATCCTGAC+TGG	0.607454	4.2:-46568583	MS.gene049757:CDS
CCATTCAAGATGGATTCACG+TGG	0.609365	4.2:-46569901	MS.gene049757:CDS
CCTCACCTTCACACCAACAC+AGG	0.610530	4.2:-46568901	MS.gene049757:CDS
ATGTTCCTTCAATGTCCCCA+TGG	0.611264	4.2:-46569410	MS.gene049757:CDS
GATGATGAAAGTGTTGCTAA+AGG	0.612075	4.2:+46577102	None:intergenic
AAATGCCAACACAAAACGAT+CGG	0.613810	4.2:+46568878	None:intergenic
ATAAATTTGGAACTGATCCG+CGG	0.618874	4.2:+46569844	None:intergenic
TGAGCAAAATCAATGTGAGA+AGG	0.619222	4.2:+46571315	None:intergenic
ATTTCGAGCACTGCAAACGA+TGG	0.623592	4.2:+46569369	None:intergenic
CCTGTGTTGGTGTGAAGGTG+AGG	0.628478	4.2:+46568901	None:intergenic
TAGAAATCTTACTTGCCACC+AGG	0.633598	4.2:+46569318	None:intergenic
GGACCTTCAGGTGAAGCCTG+AGG	0.638965	4.2:+46571883	None:intergenic
TACTTGTTAATATAATAACA+TGG	0.641143	4.2:+46569817	None:intergenic
CCACTCCATAGGTTACAGGA+AGG	0.641894	4.2:-46569945	MS.gene049757:intron
TGTTGCTAAAGGTGAAAGTG+TGG	0.646918	4.2:+46577113	None:intergenic
GAGCAATCCCTGAGACACCA+AGG	0.653614	4.2:+46571810	None:intergenic
AATCCCTGAGACACCAAGGT+AGG	0.654755	4.2:+46571814	None:intergenic
TGAACATTGGACTTCAAACA+AGG	0.656690	4.2:-46569018	MS.gene049757:CDS
CTCGCAGAAACCGAGAATGG+CGG	0.656709	4.2:-46568948	MS.gene049757:CDS
GACAAAGGAGCAAGACGATG+AGG	0.657329	4.2:+46576703	None:intergenic
GATCTTCAATCAAAGAAACT+GGG	0.658234	4.2:+46576827	None:intergenic
ACTTCAAACAAGGTATATGA+CGG	0.664094	4.2:-46569008	MS.gene049757:CDS
CCCAGAAGCTGAAACGGCGT+AGG	0.671253	4.2:+46576790	None:intergenic
GAGCTAATGAAGGGAACCCG+CGG	0.685044	4.2:-46569861	MS.gene049757:CDS
GATCGATACCAAACTCCCCG+TGG	0.697124	4.2:-46569461	MS.gene049757:CDS
TGTAAAACCAGAGTTTGCGA+CGG	0.708528	4.2:-46571397	MS.gene049757:CDS
GTTGCTAAAGGTGAAAGTGT+GGG	0.710774	4.2:+46577114	None:intergenic
GAGTCTTACCAGGTGCCATG+GGG	0.713819	4.2:+46569395	None:intergenic
ATTTCTGGTCACCAACCACG+GGG	0.751627	4.2:+46569446	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	46568420	46577148	46568420	ID=MS.gene049757
chr4.2	mRNA	46568420	46577148	46568420	ID=MS.gene049757.t1;Parent=MS.gene049757
chr4.2	exon	46576702	46577148	46576702	ID=MS.gene049757.t1.exon1;Parent=MS.gene049757.t1
chr4.2	CDS	46576702	46577148	46576702	ID=cds.MS.gene049757.t1;Parent=MS.gene049757.t1
chr4.2	exon	46571754	46571989	46571754	ID=MS.gene049757.t1.exon2;Parent=MS.gene049757.t1
chr4.2	CDS	46571754	46571989	46571754	ID=cds.MS.gene049757.t1;Parent=MS.gene049757.t1
chr4.2	exon	46571308	46571434	46571308	ID=MS.gene049757.t1.exon3;Parent=MS.gene049757.t1
chr4.2	CDS	46571308	46571434	46571308	ID=cds.MS.gene049757.t1;Parent=MS.gene049757.t1
chr4.2	exon	46569820	46569956	46569820	ID=MS.gene049757.t1.exon4;Parent=MS.gene049757.t1
chr4.2	CDS	46569820	46569956	46569820	ID=cds.MS.gene049757.t1;Parent=MS.gene049757.t1
chr4.2	exon	46569330	46569489	46569330	ID=MS.gene049757.t1.exon5;Parent=MS.gene049757.t1
chr4.2	CDS	46569330	46569489	46569330	ID=cds.MS.gene049757.t1;Parent=MS.gene049757.t1
chr4.2	exon	46568782	46569057	46568782	ID=MS.gene049757.t1.exon6;Parent=MS.gene049757.t1
chr4.2	CDS	46568782	46569057	46568782	ID=cds.MS.gene049757.t1;Parent=MS.gene049757.t1
chr4.2	exon	46568420	46568674	46568420	ID=MS.gene049757.t1.exon7;Parent=MS.gene049757.t1
chr4.2	CDS	46568420	46568674	46568420	ID=cds.MS.gene049757.t1;Parent=MS.gene049757.t1

Gene Sequence

>MS.gene049757

ATGGCACTTTCACCCACACTTTCACCTTTAGCAACACTTTCATCATCATCACCAAGAAACAAACCTCCAAAACCGTTTCTCTTTTCATCCTTCACAAAAAAGTCACAATTTTTAACAAATTCAACAAGACCCAGAAACAGAAACAGGAGAAACCTTTTAGTAACAGTTAGAAGTGTTGCAGCACCACCTACAAAAACTGAACAAAATCAATCCTACCCAGAAGCTGAAAAGAACGAAATTGATGAAGAATTTGAAGATGAGAGTTCTTCATCGAAGTTTTCATGGAGAGATCATTGGTACCCAGTTTCTTTGATTGAAGATCTGGATCCTAGTTTGCCTACGCCGTTTCAGCTTCTGGGTCGTGAAATTGTGCTTTGGTTTGATAAGGCTAATTCTCAATGGGTTGCTTTTGATGATAAATGTCCTCATCGTCTTGCTCCTTTGTCCGTAAGTGTAGTTTTTTGTTGTTGAAGATTGAATTGAGGGAAATTTAATTAAGCCATGTTTGGATAAACGGTTTAATTAAGCATTTATAGTGTATAAGTGTTTATGTATAAGTTATTTCTATAACAAAAGATAAAATAAGCTCAATTTGTTTTCATATAAACGATAAGCTATTTTCATAAGCTATCCTGAAGAACAAATGAAAATAAGTTGAAATCAGCTTATAAACATGTCACAAGCTATTTCCAGAAGCTCTTAGCCTATGTTTGGTATCACAGTAGTTATGTCAGAATCACGGTGGCCCACCTTGCTTCTACAAAGTCACGGTAGTGTTGTAAATTTTGATTATATTTCAAAAGTTTCAAATATTTTGTGTCCCATATTTTTAATTGAGAAATATAGAAAAAATATTTTGATAGAAAATATTGCTATCATATTTGACTTGGTTTTGTAAACAAGTTTTAACTCCACAATTTTGTAACGGCCTTCCTTTATAGCTCTTAAATGTGACAGTAACATTGCTTGGTCAAAGAAAATATATCTTAGTACGTAACGCTCAATTCTTTGTAAGCATGTTAATTTGTATTACTTGCTCATTATAAGAAACAAGCCGTTACTCACACCCAGGATGCACACATTCTTTTACTTGGTCTTGCCTTTTTTTTCTTTAAATAAAACGATCACTAGATTGTGCAAATATATATGAAATTAGTTGCCATGCAATAGGTGTATTCACACACCTAAACTTTCAAATCGTGGAATGCCATTTTCTTGGTCACAATTTTTACTTCATATTTTTCCTCTCCTTCCAACAATTGAGTTCTTTGGGTTACATCTTCCTGAAAATAATTTTTCTTGTTAGTTTTGTTTCCTCGATTATACATTGAGAAGGGTCTGTTAAATCCTGGGGGATTAGCGTATAATTACGCGGAAAATCCTTAAGGACAGTGCGATCAGCACGTCTCAGACTATTTCCACTATTTCTTCATCTTGCAATTCTTTTATTGAATTGATGAATAGAGATGGTGATTGATGAGTTATGTCGGTGGTCAATTCAACAACAATCTCAACACAGATAAGTTTCCTTAAAATCTTCCTTGATTATTCCTATTAACATAAATTGATTTTTATATTTATTTGTTGATTATTATTTATAGATATTCTTATCCCTAATTTTACAACAATCTTAAGGATTTTCTTTGGCATAGAATTATTAATTCTGTTTGAATTATTTGTTGATGAAAAGAAAATTACGAGAATTGAAAGTGTACTTGTAATTTAATTATTTCGTTGCTTCTCTTCGTCCAATTGATCGGTACTTGAGTAATTGTATATACATGTTTGTTATTACTGTTGATCTTTTAATATAGAACCCATGATTGATAAATATGGTTTTATCCGTAATGGTTTGTAGAGTACTTCTCCTTTGCCTTGTTAATATATAAGATGAAGTTAAACTTATATAAGTTTGCTTATACGGTTTTATTATTACGACTTTGATAATTGTTAATTGAAATTCGAATTGCTAAATTATGGTATGAAATGATTGGTTCGATTACTCTATTCGGTTGAAGAGAGAATGTATTCTTCCGGAATCAAGCCAATTTAATTTTATCAAAATGGATTCTTTAGAAGACATATAAATCACAGAACATCACAGTATTGATGCATTCATTTAATTTAGCTGCTAAAGGGACCAGGTAAGTCTCCTCGTCTTCCTTGATTTTGAGTTGATGATTATGCGTTCAAGATTGTACTCTTGATATATGAAAATTTGATGCAATAGATAAAATAAATTTTCTAAAATGGAAACTGAGTTTCTTTAAGCATGTTATAACCTCATTTGCTATATCTAATTTAATTTGATCGAGACACACATATATGCATGTTGTGAAAATTAACTGTGTTGCATTTGTTTTAAAGAAGTTGCCACAAAGTTTTACCACCATTTGTCTATGATGTAAAGTTGATCTGTTCTGCTAAAGAAATGGTAAATTATTTTGTGAAGAGATGTATGGGTATGTATGTATTGTAGATTTGTAGTTGTGTTAACAGGAGCATAAATGATTTTTGGAAGGATATCTTGGATAAATATTTAAAAAATGATACTTTCAAATTCCATTATAATTGCATACAATATTTGATGGTGCAAATTAATTCTTCCATTCGATTGTTTATCATTGTGAATGCTTTCTACTTTTTATGTTGAAATATATGGTGTGTTCATGTATGTATTATAGGATAGTAATATGTAAAGTTGTCTCTACAAAATTTACTTTGATCGGGTATATGTCATATATGTTTATATTGAGACCACTCCTTGACATGTGTATGTATGAGTTTAGTGTTTTGAAAATTTTACCTTATACAAGTGAGTTGACATGAGATATTTTATTGAGAAAATTATGAAAAATATCTTATGGGTTATCCATTGTATTGGAATAATACAATGATGTTTAATTAAATTTGTTTAGGCAAAAATATAAATTGATTAGGATTTATTTTCTCCATTGTAAGAATGTTGTCACTTGAAAATTATTCAAGTAGACCTGTATAATAAATCCACTCATAGATTTATATTTTTTCAATGTGGGGGTTTGTCACTTAGAATTTATCTAAGCAGACCTGATGAAGTATATCAAGGATGTACCATTGCATATCAAAATAATGCAATGATGTTGATTGAAATTGAAATTCAATAAGAATAAATCAAAATGTACAATTACTTTACTTATTGTGGGGATATTGTCGCTTGAAGTCATTCAAGCAGACCTGTATTATAAATCTACAAATAGATTTATATTTAGCCACATTGATATTGATGTCGCTTAATAATAATGTAAGCAGACATGTATAAAAATAATTATAGAGATCAAGTTTATTGTTATAAACTTTGATAGATTTATGAGGTCGATTGACTTAGAGACGTCTTAGTCAATCTTATATGATGAAAGAAAATTATCTTCATCATTTGTGTATAAAGTAGCTACTAAACAAAACCCCTTGGGATGAAATAAATTGTGTACATCGAGGGGATTGGGACTAAAGCCATTTGAAATTAATAAGTGATGGGAACCCAACCTTTGTGATTGGAGATCCCATGAATAAGGTTCATATGGGTAAGAACAAGTCACTTATTAGTTCTGCTAGCACCAAAAATTAGTTAATTAATTTTTGTTCTCATCCCTTTCCTATGGTGTGTGTGAGAAGTGCTAGACACTGCATTATTGAGAGGATAAGCATATAAAGCTTTTAATGAATTTCATATCCCTTATGGGTGGTGTATGATTTGCAGCATACACTTGATGAAATCACCTATGTGAGTGTCAAGTGGGGCCGCTTGTATGGGATCTTGGCGAAATCTCTGGAGCACTCATGAAATAATCCAGGCACGCGCACGGCCTATTCGCGCACCACAGCGCCAACGGCAAGAATTGTGGGGGCGTGATGCGAATGGTATACCGCTAACATACATCAAGTGATTTTGGTTCATATAGCTTGCTACACCAACTTCACTGTGTGTTAAGTATTATCATTCTAGGGCTGGTTCATATAGCTTGCTACACCAGTTTGATGCATCACATCTAAGATGTTAACCCAGGAAGTCCTTTTTCTATTTTCTTTTAAACTTTTGAAATATGTGGGGGATTGTTGTAAATTTTGATTATATTTCAAAAGTTTCAAATATTTTGTGTCCCATATTTTTAATTGAGAAATATAGAAAAAATATTTTGATAGAAAATATTGCTATCATATTTGACTTGGTTTTGTAAACAAGTTTTAACTCCACAATTTTGTAACGGCCTTCCTTTATAGCTCTTAAATGTGACAGTAACATTGCTTGGTCAAAGAAAATATATCTTAGTACGTAACGCTCAATTCTTTGTAAGCATGTTAATTTGTATTACTTGCTCATTATAAGAAACAAGCCGTTACTCACACCCAGGATGCACACATTCTTTTACTTGGTCTTGCCTTTTTTTTCTTTAAATAAAACGATCACTAGATTGTGCAAATATATATGAAATTAGTTGCCATGCAATAGGTGTATTCACACACCTAAACTTTCAAATCGTGGAATGCCATTTTCTTGGTCACAATTTTTACTTCATATTTTTCCTCTCCTTCCAACAATTGAGTTCTTTGGGTTACATCTTCCTGAAAATAATTTTTCTTGTTAGTTTTGTTTCCTCGATTATACATTGAGAAGGGTCTGTTAAATCCTGGGGGATTAGCGTATAATTACGCGGAAAATCCTTAAGGACAGTGCGATCAGCACGTCTCAGACTATTTCCACTATTTCTTCATCTTGCAATTCTTTTATTGAATTGATGAATAGAGATGGTGATTGATGAGTTATGTCGGTGGTCAATTCAACAACAATCTCAACACAGATAAGTTTCCTTAAAATCTTCCTTGATTATTCCTATTAACATAAATTGATTTTTATATTTATTTGTTGATTATTATTTATAGATATTCTTATCCCTAATTTTACAACAGGTAGGTCACGTGATTCTGACATACTTATACCAAACATACACTTACTTATAATAGTAGATGAGCTCAAATTGCATAAGCTCTTACAAGTGTTTATGCCAGTAGATGAGCTCAAATAAGTCAATTTTCATAAGCTCTTACAAGTGTTTATGCCAATAGATGTGCTCAATTAAGTCAAAGTAAACTGACCTTTATGATTTGGTTGGTTTTTGCAGGAAGGAAGGATAGATGAAAATGGAAACTTGCAGTGTTCTTATCATGGGTGGTCTTTTGATGGACGTGGAGGTTGCACTGTGATCCCTCAGGCTTCACCTGAAGGTCCGGAAGCGCGTGCTGTTGGATCTCCTAGAGCTTGTGCTACTAGGTTTCCTACCTTGGTGTCTCAGGGATTGCTCTTTGTTTGGCCTGATGAGAATGTTTGGGAGAAAGCAAATGCATCTAAACCTCCAATGTAATGATTTGTTTAATTATATATTTAGTCTAATTAATAATATTTGTTAATTTGTTTATCTTGTTGTAACTGATGCAATGAGTGTGACTCAGCTAGTCAATCAGTGCTTAAGCTACAACTTAAGCACCTGAGTTGATATAAGTCCATAATAGGTTTAATGATCTAAATTTACGAAAATATTTCGAGCTAAATACCCTAATTACGGTGGTTTCTTAGAAAAGTTGTTGTGATTGGGCCACTTAAAAATGTTATCTTATAGTATTAAAGAGTGTTTGTTGTATACTTTTATTGATCTGTTTGGTAAAATTTTGTATGTTAGGTTGCCTGATGACTTTGTAAAACCAGAGTTTGCGACGGTGAACATTCAGCGCGATCTTTTCTACGGCTATGATACTCTCATGGAGAATGTTTCTGATCCTTCTCACATTGATTTTGCTCATCACAAGGTACTAATGACACACTTCAACTTGACTTTATGGTGTGCTGGTTATTTTATATTCAAACAGTGTGCTTGTTGATAATATTTTATTAAGATATTATTGTAAGTGGAATTTGTTGAATTAAATATACTAAGTGATGTTGATGTGGTCCAAAGATTGTGAAGGGAGGGCCTTTTGGTTTGTTCCATTTTTATATATTTTCACTCACTGAAACTGCTGCTTGCTCAGCATATTTTATATATCTTCACTCACTAATATTATATTGCAATCAAGAATTTAGTCAAGGACAGTGCTTTTTTAGGTTTAGAAACTAGTTGCTTGTAATTAATTGTTTTTTATTTCATTTATTCCAAATTATTTAAATTTAATCACTTAAGATTGGACCTTTTTAACAAAACAAAAAGGGAAATTGATAAGTACCCAACCACCCGGGGAACCCTCCCTTAAAAGCTAGACATCAAGAAGGAAGAAACTCCACCCCCAAACATCCCAGGAGAGCCGATGTCCCAGTGACTGCTCTCTATGATACCTTACTTGGTCTTTCCGGTCAGCGCCTCCATAGGGAGCCCTTTCTAAGACACTGAAAACCAAGATCTTATACCAATTTATAAGTACCCAAGCACTTGAGGAACTCTTCCTTAAAAGCTAGCTATCAAGGGGAAAAACCAAGCAACATAAATACACCATCAAGCATTCCATTCAGGGACGGATGCATATTGTTACATGTGCAGGCTTGTGCCTACACAAAATTTAGAAACACTTTTTTTTAGTAGTATAATGTACCAATAGTTGACGTTATGACTACGTAGCTCACACTTATCACAAATTATTTCTCTCTTGGTCCACGCCTATCATCATATATCTCAAATCCTTTCTTAATAATATGAATATTATAAAAAATTGGATGCGAAATCGAGTGAGAGATGACAAGTTAAATGATTGTTTAGTTATTTATATAGAGAAACATATTTTTATTGATATTCAAAATGAGATATCATTCAAAATTTACATAATATAAAAAATTGGTACCCTCACTATTCAAAAATCCTGGATCCGTCGCTGATTCCATACTACTTGATGTTTGAGAGGAAGTGCAAGCAGAAAAACAGTGAGGCGGTATAGGAGATGGGTGGGTGCAATTTCGTTGTACAATCATATTGTGTCTTAATGATTTATGGTGCACAGTCAATTTCCCTTTTGGATTTGCTAAATTTAGATTGATTGTCAATGTTTGTCTATCATTTTGCACATTAGTATGTGTTATTAAGACACTATCAAGATAAGAAGTTGAAATTATTTAGTATTATTTGTTAGGGTTATTTAAATCACTAACATAAATATGATTTCCACTCCATAGGTTACAGGAAGGAGAGACAGAGCCAAACCTCTTCCATTCAAGATGGATTCACGTGGTTCATGGGGCTTTTCAGGAGCTAATGAAGGGAACCCGCGGATCAGTTCCAAATTTATTGCACCATGTTATTATATTAACAAGTAAGCCAGTGGCATTTGCATGAAAGTACTTTTCATACGAATTCTGGTTTCATTTTCCCATTTTACATTATTTGATGCCATCTTAAATGATGAGTAACTTAATATGAATTATGTACTTGTTCATGGCCATAAAAGAATAGTTTTAATTTTTAATTGAAAAAGGACGGTATCTACCTCGATATTTGGATGATTGCGGCATCATGCGGCTAATTGTGTCTTGTTTGTATGCTATATTTCAAGATGCATTTCAAAAGATATTACCTAGCCTTGTCACTGTTTTCTTTCCATTTGTTGGTTCTGGCTCAGTAAATTAAGAACTATTTTTTTCAGGATTGCGATCGATACCAAACTCCCCGTGGTTGGTGACCAGAAATGGGTAATATGGATATGTTCCTTCAATGTCCCCATGGCACCTGGTAAGACTCGCTCCATCGTTTGCAGTGCTCGAAATTTCTTCCAGCTCACGGTGCCAGGGCCTGCCTGGTGGCAAGTAAGATTTCTATATTCAATGCTTTCGTTTCCATTCTCTTCGATAAAACCACGTGAATGTTTCATTGACAGAAATTGAGATACAATTTTAGCAAACCTTGACAGAAATTATTCGCTGTTATCGTTGAAGAATGTTATGTTTCTTTCTATGAAACCATTTTATTCGGAAGATTTTTAAACAAATTGGAATTCTTGAACCTCTAATATCGACTTCTAGGATAAAATATTTCCAGGAACAAGCAAATCCTAACGATTTTCTTGTTCATGTCTCAGGTGGTTCCTAGATGGTATGAACATTGGACTTCAAACAAGGTATATGACGGTGACATGATTGTACTTCAAGGTCAAGAGAAAGTCTTTCTCGCAGAAACCGAGAATGGCGGTGACATTAACAAACAGTACACAAGCCTCACCTTCACACCAACACAGGCCGATCGTTTTGTGTTGGCATTTAGAAATTGGCTGAGGCGACACGGCAATGGCCAACCTGAATGGTTCGGCAAGAGCAGCAGCCAGCCATTGCCTTCAACCGTACTATCAAAACGTCAGGTATAGCAATTTGATTAAGTTATTTCATTATGAACCGTTTTCCATTGTCTACAAAACCAAGAATAAAACACAACTTTGATGATCTTTTGTGTTTGTTTTTCATGTAGATGTTGGATAGATTTGAACAGCACACACTAAAGTGTTCAGCTTGTAAAGGAGCTTATGAGGGATTCCAAACATGGCAGAAAATCCTGACTGGTGCAACAGTTGTGTTTTGCGCGACTTCTGGTATTCCATCAGATACTCAGCTGCGCATAGTTTTGGCTGCACTCGCGCTTGTCAGCGCAGCCTTAGCTTTTTCCATAAACCAACTGAAAAACAATTTTGTTTTCGTCGATTATGTGCACGCTGAAATCGATTGA

Protein sequence

>MS.gene049757.t1

MALSPTLSPLATLSSSSPRNKPPKPFLFSSFTKKSQFLTNSTRPRNRNRRNLLVTVRSVAAPPTKTEQNQSYPEAEKNEIDEEFEDESSSSKFSWRDHWYPVSLIEDLDPSLPTPFQLLGREIVLWFDKANSQWVAFDDKCPHRLAPLSEGRIDENGNLQCSYHGWSFDGRGGCTVIPQASPEGPEARAVGSPRACATRFPTLVSQGLLFVWPDENVWEKANASKPPMLPDDFVKPEFATVNIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKMDSRGSWGFSGANEGNPRISSKFIAPCYYINKIAIDTKLPVVGDQKWVIWICSFNVPMAPGKTRSIVCSARNFFQLTVPGPAWWQVVPRWYEHWTSNKVYDGDMIVLQGQEKVFLAETENGGDINKQYTSLTFTPTQADRFVLAFRNWLRRHGNGQPEWFGKSSSQPLPSTVLSKRQMLDRFEQHTLKCSACKGAYEGFQTWQKILTGATVVFCATSGIPSDTQLRIVLAALALVSAALAFSINQLKNNFVFVDYVHAEID