Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71734.t1 | XP_003592570.1 | 98.8 | 173 | 2 | 0 | 1 | 173 | 1 | 173 | 6.60E-89 | 336.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71734.t1 | G7IAV0 | 98.8 | 173 | 2 | 0 | 1 | 173 | 1 | 173 | 4.7e-89 | 336.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene71734 | 0.866695 | 2.36E-65 | -1.69E-46 |
| MS.gene051226 | MS.gene71734 | 0.868247 | 7.53E-66 | -1.69E-46 |
| MS.gene051837 | MS.gene71734 | 0.898658 | 4.28E-77 | -1.69E-46 |
| MS.gene057093 | MS.gene71734 | 0.868512 | 6.18E-66 | -1.69E-46 |
| MS.gene057432 | MS.gene71734 | 0.836503 | 9.01E-57 | -1.69E-46 |
| MS.gene06061 | MS.gene71734 | 0.877993 | 4.02E-69 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71734.t1 | MTR_1g108640 | 98.844 | 173 | 2 | 0 | 1 | 173 | 1 | 173 | 9.58e-125 | 348 |
| MS.gene71734.t1 | MTR_2g105800 | 46.452 | 155 | 69 | 4 | 18 | 170 | 13 | 155 | 6.14e-37 | 125 |
| MS.gene71734.t1 | MTR_8g107420 | 33.708 | 178 | 98 | 4 | 1 | 171 | 1 | 165 | 5.44e-17 | 74.3 |
| MS.gene71734.t1 | MTR_4g068850 | 26.667 | 105 | 75 | 1 | 20 | 124 | 21 | 123 | 1.60e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71734.t1 | AT3G03280 | 41.111 | 180 | 81 | 6 | 1 | 170 | 1 | 165 | 5.80e-36 | 123 |
| MS.gene71734.t1 | AT5G17350 | 40.957 | 188 | 87 | 6 | 1 | 170 | 1 | 182 | 1.98e-32 | 114 |
Find 37 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGATCAAAGAAACCTATTT+TGG | 0.162510 | 1.1:-77621575 | MS.gene71734:CDS |
| TTCCTTTATCTTGACCTAAT+TGG | 0.198618 | 1.1:+77621535 | None:intergenic |
| GCAGATGAAGAATTGGAATT+TGG | 0.234414 | 1.1:-77621841 | MS.gene71734:CDS |
| CGGCTGAACACGTGTCTTTC+CGG | 0.234431 | 1.1:+77621711 | None:intergenic |
| GAGTTGACTAAGAAATAATT+TGG | 0.256837 | 1.1:+77621904 | None:intergenic |
| TGGAATCAGGGTTTAGTTAT+AGG | 0.257374 | 1.1:-77621609 | MS.gene71734:CDS |
| AGTTTGGAAGGTGTGGAATC+AGG | 0.279397 | 1.1:-77621622 | MS.gene71734:CDS |
| TCATTTATAGTCTCCAAAAT+AGG | 0.290361 | 1.1:+77621562 | None:intergenic |
| TCTTGCTGCAGATGAAGAAT+TGG | 0.387242 | 1.1:-77621848 | MS.gene71734:CDS |
| AACCAATTAGGTCAAGATAA+AGG | 0.404180 | 1.1:-77621537 | None:intergenic |
| GTTTGGAAGGTGTGGAATCA+GGG | 0.418181 | 1.1:-77621621 | MS.gene71734:CDS |
| AATTGTTTCACTTCTCCTGT+TGG | 0.426516 | 1.1:+77621967 | None:intergenic |
| CTATAAATGAAGAACCAATT+AGG | 0.456816 | 1.1:-77621549 | MS.gene71734:CDS |
| ATGTGTTCCGAGGTTGAGTT+TGG | 0.464828 | 1.1:-77621638 | MS.gene71734:CDS |
| TGTTCAGCCGGATGAGAGTT+CGG | 0.467508 | 1.1:-77621698 | MS.gene71734:CDS |
| ACAGAATTGAGTCTTCTCAT+AGG | 0.502486 | 1.1:+77621799 | None:intergenic |
| CCTCATCAACGGTGGTGCTA+AGG | 0.505204 | 1.1:+77622017 | None:intergenic |
| CAGCACCGCCATGTCAGCTC+CGG | 0.507579 | 1.1:+77621771 | None:intergenic |
| GTTTCACTTCTCCTGTTGGA+AGG | 0.509220 | 1.1:+77621971 | None:intergenic |
| ACAAGAGTAATCCTTCCAAC+AGG | 0.510852 | 1.1:-77621982 | MS.gene71734:CDS |
| TGTGGTCACCGGAGCTGACA+TGG | 0.524219 | 1.1:-77621779 | MS.gene71734:CDS |
| CGGAAAGACACGTGTTCAGC+CGG | 0.528707 | 1.1:-77621710 | MS.gene71734:CDS |
| TTCACTTCCGAACTCTCATC+CGG | 0.533475 | 1.1:+77621691 | None:intergenic |
| GGATGAGAGTTCGGAAGTGA+AGG | 0.539299 | 1.1:-77621689 | MS.gene71734:CDS |
| CGGCGAATTCGGCTGCGAAG+CGG | 0.542084 | 1.1:-77621741 | MS.gene71734:CDS |
| GCTGCGAAGCGGTTACGTGC+CGG | 0.545063 | 1.1:-77621730 | MS.gene71734:CDS |
| AATTCTTCATCTGCAGCAAG+AGG | 0.547180 | 1.1:+77621850 | None:intergenic |
| AGACTCAATTCTGTGGTCAC+CGG | 0.558052 | 1.1:-77621790 | MS.gene71734:CDS |
| GAGGTTGAGTTTGGAAGGTG+TGG | 0.563066 | 1.1:-77621629 | MS.gene71734:CDS |
| CCTTAGCACCACCGTTGATG+AGG | 0.584450 | 1.1:-77622017 | MS.gene71734:CDS |
| GTTCCGAGGTTGAGTTTGGA+AGG | 0.607016 | 1.1:-77621634 | MS.gene71734:CDS |
| GATGAGAGTTCGGAAGTGAA+GGG | 0.620669 | 1.1:-77621688 | MS.gene71734:CDS |
| ACACCTTCCAAACTCAACCT+CGG | 0.627609 | 1.1:+77621631 | None:intergenic |
| TATGAGAAGACTCAATTCTG+TGG | 0.636561 | 1.1:-77621797 | MS.gene71734:CDS |
| TTTGCGTTCCTCATCAACGG+TGG | 0.639490 | 1.1:+77622009 | None:intergenic |
| ATCAAAATGAATGTGTTCCG+AGG | 0.670622 | 1.1:-77621648 | MS.gene71734:CDS |
| GGTCACCGGAGCTGACATGG+CGG | 0.787053 | 1.1:-77621776 | MS.gene71734:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTATAAATGAAGAACCAATT+AGG | - | chr1.1:77622030-77622049 | MS.gene71734:CDS | 25.0% |
| ! | GAGTTGACTAAGAAATAATT+TGG | + | chr1.1:77621678-77621697 | None:intergenic | 25.0% |
| ! | TCATTTATAGTCTCCAAAAT+AGG | + | chr1.1:77622020-77622039 | None:intergenic | 25.0% |
| !! | TAGATCAAAGAAACCTATTT+TGG | - | chr1.1:77622004-77622023 | MS.gene71734:CDS | 25.0% |
| AATTGTTTCACTTCTCCTGT+TGG | + | chr1.1:77621615-77621634 | None:intergenic | 35.0% | |
| ACAGAATTGAGTCTTCTCAT+AGG | + | chr1.1:77621783-77621802 | None:intergenic | 35.0% | |
| ATCAAAATGAATGTGTTCCG+AGG | - | chr1.1:77621931-77621950 | MS.gene71734:CDS | 35.0% | |
| GCAGATGAAGAATTGGAATT+TGG | - | chr1.1:77621738-77621757 | MS.gene71734:CDS | 35.0% | |
| TATGAGAAGACTCAATTCTG+TGG | - | chr1.1:77621782-77621801 | MS.gene71734:CDS | 35.0% | |
| TGGAATCAGGGTTTAGTTAT+AGG | - | chr1.1:77621970-77621989 | MS.gene71734:CDS | 35.0% | |
| AATTCTTCATCTGCAGCAAG+AGG | + | chr1.1:77621732-77621751 | None:intergenic | 40.0% | |
| ACAAGAGTAATCCTTCCAAC+AGG | - | chr1.1:77621597-77621616 | MS.gene71734:CDS | 40.0% | |
| TCTTGCTGCAGATGAAGAAT+TGG | - | chr1.1:77621731-77621750 | MS.gene71734:CDS | 40.0% | |
| ACACCTTCCAAACTCAACCT+CGG | + | chr1.1:77621951-77621970 | None:intergenic | 45.0% | |
| AGACTCAATTCTGTGGTCAC+CGG | - | chr1.1:77621789-77621808 | MS.gene71734:CDS | 45.0% | |
| ATGTGTTCCGAGGTTGAGTT+TGG | - | chr1.1:77621941-77621960 | MS.gene71734:CDS | 45.0% | |
| GATGAGAGTTCGGAAGTGAA+GGG | - | chr1.1:77621891-77621910 | MS.gene71734:CDS | 45.0% | |
| GTTTCACTTCTCCTGTTGGA+AGG | + | chr1.1:77621611-77621630 | None:intergenic | 45.0% | |
| TTCACTTCCGAACTCTCATC+CGG | + | chr1.1:77621891-77621910 | None:intergenic | 45.0% | |
| ! | AGTTTGGAAGGTGTGGAATC+AGG | - | chr1.1:77621957-77621976 | MS.gene71734:CDS | 45.0% |
| ! | GCTTTTGCGTTCCTCATCAA+CGG | + | chr1.1:77621576-77621595 | None:intergenic | 45.0% |
| ! | GTTTGGAAGGTGTGGAATCA+GGG | - | chr1.1:77621958-77621977 | MS.gene71734:CDS | 45.0% |
| GAGGTTGAGTTTGGAAGGTG+TGG | - | chr1.1:77621950-77621969 | MS.gene71734:CDS | 50.0% | |
| GGATGAGAGTTCGGAAGTGA+AGG | - | chr1.1:77621890-77621909 | MS.gene71734:CDS | 50.0% | |
| GTTCCGAGGTTGAGTTTGGA+AGG | - | chr1.1:77621945-77621964 | MS.gene71734:CDS | 50.0% | |
| TGTTCAGCCGGATGAGAGTT+CGG | - | chr1.1:77621881-77621900 | MS.gene71734:CDS | 50.0% | |
| TTTGCGTTCCTCATCAACGG+TGG | + | chr1.1:77621573-77621592 | None:intergenic | 50.0% | |
| !!! | GCTGTTTTTGGCGGCGAATT+CGG | - | chr1.1:77621827-77621846 | MS.gene71734:CDS | 50.0% |
| !!! | TGACATGGCGGTGCTGTTTT+TGG | - | chr1.1:77621815-77621834 | MS.gene71734:CDS | 50.0% |
| CCTTAGCACCACCGTTGATG+AGG | - | chr1.1:77621562-77621581 | MS.gene71734:CDS | 55.0% | |
| CGGAAAGACACGTGTTCAGC+CGG | - | chr1.1:77621869-77621888 | MS.gene71734:CDS | 55.0% | |
| CGGCTGAACACGTGTCTTTC+CGG | + | chr1.1:77621871-77621890 | None:intergenic | 55.0% | |
| ! | CCTCATCAACGGTGGTGCTA+AGG | + | chr1.1:77621565-77621584 | None:intergenic | 55.0% |
| !!! | CATGGCGGTGCTGTTTTTGG+CGG | - | chr1.1:77621818-77621837 | MS.gene71734:CDS | 55.0% |
| TGTGGTCACCGGAGCTGACA+TGG | - | chr1.1:77621800-77621819 | MS.gene71734:CDS | 60.0% | |
| CAGCACCGCCATGTCAGCTC+CGG | + | chr1.1:77621811-77621830 | None:intergenic | 65.0% | |
| CGGCGAATTCGGCTGCGAAG+CGG | - | chr1.1:77621838-77621857 | MS.gene71734:CDS | 65.0% | |
| GCTGCGAAGCGGTTACGTGC+CGG | - | chr1.1:77621849-77621868 | MS.gene71734:CDS | 65.0% | |
| GGTCACCGGAGCTGACATGG+CGG | - | chr1.1:77621803-77621822 | MS.gene71734:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 77621540 | 77622061 | 77621540 | ID=MS.gene71734 |
| chr1.1 | mRNA | 77621540 | 77622061 | 77621540 | ID=MS.gene71734.t1;Parent=MS.gene71734 |
| chr1.1 | exon | 77621540 | 77622061 | 77621540 | ID=MS.gene71734.t1.exon1;Parent=MS.gene71734.t1 |
| chr1.1 | CDS | 77621540 | 77622061 | 77621540 | ID=cds.MS.gene71734.t1;Parent=MS.gene71734.t1 |
| Gene Sequence |
| Protein sequence |
