Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72031.t1 | XP_003603638.1 | 96.5 | 173 | 6 | 0 | 1 | 173 | 1 | 173 | 2.10E-95 | 358.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72031.t1 | P59082 | 35.8 | 162 | 90 | 6 | 8 | 168 | 20 | 168 | 4.7e-19 | 95.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72031.t1 | G7J3I2 | 96.5 | 173 | 6 | 0 | 1 | 173 | 1 | 173 | 1.5e-95 | 358.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene64289 | MS.gene72031 | PPI |
| MS.gene72031 | MS.gene94983 | PPI |
| MS.gene72031 | MS.gene064976 | PPI |
| MS.gene72031 | MS.gene53620 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72031.t1 | MTR_3g109880 | 96.532 | 173 | 6 | 0 | 1 | 173 | 1 | 173 | 5.26e-123 | 343 |
| MS.gene72031.t1 | MTR_6g088445 | 42.945 | 163 | 82 | 3 | 3 | 164 | 5 | 157 | 9.83e-42 | 138 |
| MS.gene72031.t1 | MTR_6g088475 | 39.877 | 163 | 87 | 3 | 3 | 164 | 5 | 157 | 2.86e-38 | 129 |
| MS.gene72031.t1 | MTR_1g046660 | 35.429 | 175 | 104 | 3 | 1 | 173 | 1 | 168 | 5.60e-34 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72031.t1 | AT4G32870 | 49.701 | 167 | 70 | 4 | 1 | 165 | 1 | 155 | 3.55e-56 | 174 |
| MS.gene72031.t1 | AT2G25770 | 39.759 | 166 | 88 | 4 | 1 | 164 | 1 | 156 | 4.71e-38 | 128 |
| MS.gene72031.t1 | AT2G25770 | 39.759 | 166 | 88 | 4 | 1 | 164 | 1 | 156 | 4.71e-38 | 128 |
Find 40 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGACTTTACCTTCCCATTT+TGG | 0.196562 | 3.4:+95507714 | None:intergenic |
| TATTGTGCTTCTAACATAAA+AGG | 0.298692 | 3.4:-95507558 | MS.gene72031:CDS |
| GAGAGGAATCTATACCAAAA+TGG | 0.300079 | 3.4:-95507728 | MS.gene72031:CDS |
| TGTAATGTCCACCCTTCTAT+TGG | 0.303893 | 3.4:+95507318 | None:intergenic |
| TGAGTACTGTCTTCAGTTCA+TGG | 0.311338 | 3.4:-95507277 | MS.gene72031:CDS |
| ACACCGTTGAAGAACAAGTA+TGG | 0.334958 | 3.4:-95507677 | MS.gene72031:CDS |
| TGCCCACTTGATTGTTGTCT+CGG | 0.360004 | 3.4:+95507506 | None:intergenic |
| TAGGACAAACAACGTTTGAT+TGG | 0.364301 | 3.4:+95507459 | None:intergenic |
| ACATGGTAACATGTGTCTAT+TGG | 0.380795 | 3.4:+95507615 | None:intergenic |
| GTTGTTATCAACAATTTCAT+AGG | 0.400837 | 3.4:+95507440 | None:intergenic |
| TTTGTTTGTGATCCAATAGA+AGG | 0.405699 | 3.4:-95507330 | MS.gene72031:CDS |
| ATTTCTGCAACCTACACAAA+TGG | 0.433647 | 3.4:-95507641 | MS.gene72031:CDS |
| AGAGGAATCTATACCAAAAT+GGG | 0.442772 | 3.4:-95507727 | MS.gene72031:CDS |
| TACCATGTTGAAGGTGTTCA+AGG | 0.460746 | 3.4:-95507600 | MS.gene72031:CDS |
| GAAGGTGATGCTAAAAGTGC+AGG | 0.494052 | 3.4:-95507372 | MS.gene72031:CDS |
| GTTCATGGCTAAGAAGATTG+AGG | 0.515780 | 3.4:-95507262 | MS.gene72031:CDS |
| GACACATGTTACCATGTTGA+AGG | 0.516605 | 3.4:-95507609 | MS.gene72031:CDS |
| GTGCAGGGTGTGGGATTGAG+TGG | 0.523829 | 3.4:-95507356 | MS.gene72031:CDS |
| CTATTGGAATCCATTTGTGT+AGG | 0.531264 | 3.4:+95507631 | None:intergenic |
| TGCCCATACTTGTTCTTCAA+CGG | 0.556176 | 3.4:+95507674 | None:intergenic |
| GAAATTGTTGATAACAACAT+GGG | 0.560379 | 3.4:-95507435 | MS.gene72031:CDS |
| GAAGGTGTTCAAGGCCAACC+TGG | 0.564455 | 3.4:-95507591 | MS.gene72031:CDS |
| CACCGTTGAAGAACAAGTAT+GGG | 0.572196 | 3.4:-95507676 | MS.gene72031:CDS |
| GCACAATAGCGTATGAGACC+AGG | 0.576223 | 3.4:+95507573 | None:intergenic |
| ATGCTAAAAGTGCAGGGTGT+GGG | 0.582963 | 3.4:-95507365 | MS.gene72031:CDS |
| TTGTTTGTGATCCAATAGAA+GGG | 0.583616 | 3.4:-95507329 | MS.gene72031:CDS |
| GGCCTTGAACACCTTCAACA+TGG | 0.588245 | 3.4:+95507598 | None:intergenic |
| TGCAGGGTGTGGGATTGAGT+GGG | 0.598045 | 3.4:-95507355 | MS.gene72031:CDS |
| TGAAATTGTTGATAACAACA+TGG | 0.609388 | 3.4:-95507436 | MS.gene72031:CDS |
| TTTGTGATCCAATAGAAGGG+TGG | 0.611273 | 3.4:-95507326 | MS.gene72031:CDS |
| GATGCTAAAAGTGCAGGGTG+TGG | 0.614021 | 3.4:-95507366 | MS.gene72031:CDS |
| AGTCCGAGACAACAATCAAG+TGG | 0.635290 | 3.4:-95507509 | MS.gene72031:CDS |
| ATCGGCGAGAGAGATGGGAG+AGG | 0.639114 | 3.4:-95507745 | None:intergenic |
| AAATTGTTGATAACAACATG+GGG | 0.648061 | 3.4:-95507434 | MS.gene72031:CDS |
| CTGAAAGTGCTTCCAAACGA+AGG | 0.654326 | 3.4:-95507390 | MS.gene72031:CDS |
| GAATCTATACCAAAATGGGA+AGG | 0.656789 | 3.4:-95507723 | MS.gene72031:CDS |
| GTCCGAGACAACAATCAAGT+GGG | 0.661405 | 3.4:-95507508 | MS.gene72031:CDS |
| AAGGTGATGCTAAAAGTGCA+GGG | 0.666240 | 3.4:-95507371 | MS.gene72031:CDS |
| GGAAGGTAAAGTCACTGTTG+AGG | 0.667190 | 3.4:-95507706 | MS.gene72031:CDS |
| AATAGCGTATGAGACCAGGT+TGG | 0.712406 | 3.4:+95507577 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTTGTTATCAACAATTTCAT+AGG | + | chr3.4:95507528-95507547 | None:intergenic | 25.0% |
| ! | TATTGTGCTTCTAACATAAA+AGG | - | chr3.4:95507407-95507426 | MS.gene72031:CDS | 25.0% |
| !!! | AAATTGTTGATAACAACATG+GGG | - | chr3.4:95507531-95507550 | MS.gene72031:CDS | 25.0% |
| !!! | GAAATTGTTGATAACAACAT+GGG | - | chr3.4:95507530-95507549 | MS.gene72031:CDS | 25.0% |
| !!! | TGAAATTGTTGATAACAACA+TGG | - | chr3.4:95507529-95507548 | MS.gene72031:CDS | 25.0% |
| AGAGGAATCTATACCAAAAT+GGG | - | chr3.4:95507238-95507257 | MS.gene72031:CDS | 30.0% | |
| TTGTTTGTGATCCAATAGAA+GGG | - | chr3.4:95507636-95507655 | MS.gene72031:CDS | 30.0% | |
| TTTGTTTGTGATCCAATAGA+AGG | - | chr3.4:95507635-95507654 | MS.gene72031:CDS | 30.0% | |
| ACATGGTAACATGTGTCTAT+TGG | + | chr3.4:95507353-95507372 | None:intergenic | 35.0% | |
| ATTTCTGCAACCTACACAAA+TGG | - | chr3.4:95507324-95507343 | MS.gene72031:CDS | 35.0% | |
| CTATTGGAATCCATTTGTGT+AGG | + | chr3.4:95507337-95507356 | None:intergenic | 35.0% | |
| GAATCTATACCAAAATGGGA+AGG | - | chr3.4:95507242-95507261 | MS.gene72031:CDS | 35.0% | |
| GAGAGGAATCTATACCAAAA+TGG | - | chr3.4:95507237-95507256 | MS.gene72031:CDS | 35.0% | |
| TAGGACAAACAACGTTTGAT+TGG | + | chr3.4:95507509-95507528 | None:intergenic | 35.0% | |
| ACACCGTTGAAGAACAAGTA+TGG | - | chr3.4:95507288-95507307 | MS.gene72031:CDS | 40.0% | |
| CACCGTTGAAGAACAAGTAT+GGG | - | chr3.4:95507289-95507308 | MS.gene72031:CDS | 40.0% | |
| GACACATGTTACCATGTTGA+AGG | - | chr3.4:95507356-95507375 | MS.gene72031:CDS | 40.0% | |
| GTTCATGGCTAAGAAGATTG+AGG | - | chr3.4:95507703-95507722 | MS.gene72031:CDS | 40.0% | |
| TGCCCATACTTGTTCTTCAA+CGG | + | chr3.4:95507294-95507313 | None:intergenic | 40.0% | |
| TGTAATGTCCACCCTTCTAT+TGG | + | chr3.4:95507650-95507669 | None:intergenic | 40.0% | |
| TTTGTGATCCAATAGAAGGG+TGG | - | chr3.4:95507639-95507658 | MS.gene72031:CDS | 40.0% | |
| ! | AAGGTGATGCTAAAAGTGCA+GGG | - | chr3.4:95507594-95507613 | MS.gene72031:CDS | 40.0% |
| ! | CTTTTAGCATCACCTTCGTT+TGG | + | chr3.4:95507590-95507609 | None:intergenic | 40.0% |
| ! | GTGACTTTACCTTCCCATTT+TGG | + | chr3.4:95507254-95507273 | None:intergenic | 40.0% |
| ! | TGAGTACTGTCTTCAGTTCA+TGG | - | chr3.4:95507688-95507707 | MS.gene72031:CDS | 40.0% |
| !! | TACCATGTTGAAGGTGTTCA+AGG | - | chr3.4:95507365-95507384 | MS.gene72031:CDS | 40.0% |
| AATAGCGTATGAGACCAGGT+TGG | + | chr3.4:95507391-95507410 | None:intergenic | 45.0% | |
| AGTCCGAGACAACAATCAAG+TGG | - | chr3.4:95507456-95507475 | MS.gene72031:CDS | 45.0% | |
| GGAAGGTAAAGTCACTGTTG+AGG | - | chr3.4:95507259-95507278 | MS.gene72031:CDS | 45.0% | |
| GTCCGAGACAACAATCAAGT+GGG | - | chr3.4:95507457-95507476 | MS.gene72031:CDS | 45.0% | |
| TGCCCACTTGATTGTTGTCT+CGG | + | chr3.4:95507462-95507481 | None:intergenic | 45.0% | |
| ! | ATGCTAAAAGTGCAGGGTGT+GGG | - | chr3.4:95507600-95507619 | MS.gene72031:CDS | 45.0% |
| ! | GAAGGTGATGCTAAAAGTGC+AGG | - | chr3.4:95507593-95507612 | MS.gene72031:CDS | 45.0% |
| !! | CTGAAAGTGCTTCCAAACGA+AGG | - | chr3.4:95507575-95507594 | MS.gene72031:CDS | 45.0% |
| GCACAATAGCGTATGAGACC+AGG | + | chr3.4:95507395-95507414 | None:intergenic | 50.0% | |
| GGCCTTGAACACCTTCAACA+TGG | + | chr3.4:95507370-95507389 | None:intergenic | 50.0% | |
| ! | GATGCTAAAAGTGCAGGGTG+TGG | - | chr3.4:95507599-95507618 | MS.gene72031:CDS | 50.0% |
| TGCAGGGTGTGGGATTGAGT+GGG | - | chr3.4:95507610-95507629 | MS.gene72031:CDS | 55.0% | |
| !! | GAAGGTGTTCAAGGCCAACC+TGG | - | chr3.4:95507374-95507393 | MS.gene72031:CDS | 55.0% |
| GTGCAGGGTGTGGGATTGAG+TGG | - | chr3.4:95507609-95507628 | MS.gene72031:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 95507233 | 95507754 | 95507233 | ID=MS.gene72031 |
| chr3.4 | mRNA | 95507233 | 95507754 | 95507233 | ID=MS.gene72031.t1;Parent=MS.gene72031 |
| chr3.4 | exon | 95507233 | 95507754 | 95507233 | ID=MS.gene72031.t1.exon1;Parent=MS.gene72031.t1 |
| chr3.4 | CDS | 95507233 | 95507754 | 95507233 | ID=cds.MS.gene72031.t1;Parent=MS.gene72031.t1 |
| Gene Sequence |
| Protein sequence |
